MC04g_new0575 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g_new0575
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	WAT1-related protein
Location	MC04: 23226066 .. 23229998 (-)
RNA-Seq Expression	MC04g_new0575
Synteny	MC04g_new0575

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAACAATTTGAGTAACGTTTTGAAGAAGGCGAAGCCATATTTAGCTGCCATCCTTCTAAGATTTCTGTCTGCAGCTTTGGTTGTTACAGCAAAGATTGCATTGAACCATGGCATGAGCCCCTTTGTGTATTCCCTCTATCGTTTTTTTGTTGCCGCCATTGTTGTAGCTCCATTTGCCTTACACTTTGACAGGTTTCTCTCTCTCTCTATACATCTCTTTCTCTCTCTTTGGACTTGGAAAATGGCTGAAACTGATGACACAAATAATATATATGGCAGAAAAAGAAGACCACAGATGACTTGGCCCATCTTTGCCAAGATTCTATTGCTCGGCTCTATGGAGTGAGAACTAATTTTTTTCTTAAATTATTTATTTTCATTTTCTCATACAAAATTTTAGTTTTGCGACGATATAATTTTTTAAACATTTTGATATTTACAATTCGGGTATGGTTTTAATTTCATTTCTATAAACTCGGGTTTAGCTTAATTTCAGTTTGATTTTGATAGTTTTGTACTTAATTTTAATATTTAAAACAATGTGACCTTAAAAGTTCCAATTTAATTCATATGATATGGACTTAATTTTTAATTTTTATTGTCATATAAAATACACGACACTTTAAAAAAAAATTGTGACAATGAGGAAATGCTTTCTATATCGTTAACTTTATTAAGTAAATATTTAAAAAAATGAAAAGAAGGAAAAACTTTATTAATTATCGAGTGGGTAAAAGACAAATTATATGCTATACATGGGTGAGATTTAACAAAATTACGTGGGAACTAAAAAATTATTAAGATTAAATAGAAACTTTTCAAATCATAGGACTAATAATGGAAATTAGGACCAAATTTGTATATGATTGGTTTAATTTTTGTGGTAAATGATTGATTGGGAATATTGCAGCTCTTTGGTTATCCCCAACATGTATTTCAGTGCTCTGAAATATGTAACTCCAACTTTCTCCATTGCTATGTCCAACGTTGTTCCTGCCTTTTCCTTCTTCTTCGCTGTCATTTTCAGGTACTCCTTTTTGGTTTCTGTTTAATAAATTTTTATTTTTTGTTCTTTTGTTGTTACGTTCCATGTATTTTTTTTTTACTATAAAATCTAAGCTAAATTTAAAAAATCACTCGATTTTGCTTTTGAAATATGCATAAAAGTCATACTTTTTTTCAAGAAATGTGAATAAACATACTAAAAAATTTGTGAGCTAAAGCATAATTTTCAAAAACATAGAACAAAAAAGGAAAACTCATCCCACATACCATTAACTACTGATTTACTATTTATCTATTTTTCTTACTTTTAGGTTTCTCGAGGTAAAAACTGACTTTTTTTTTTGGATAGGATGGAGAAGGTTGATATTAGGAGACTTTCATCCCAGGCAAAGATATTGGGAACTATAGTGGCAGTGGGAGGGGCAATGATAATGACCTTTTTTGGAGGCCCAAACTTGAGATTTCCTTGGACAAAATTAGAACATTTACACAATCACTCTAATTCAACTCCTCCTTCAAACACCCATCAAGATTCTTTGAAGGGTGTCATTTTGACCACAATTGCTTGTATCTGCTCTTCTGTTTCATGCATTCTCCAGGTACAAACAATACAAAAGATCATACTTCGATTCAAATATATCTTTACTCGTGTACTGTGAATCGATCTCTCGACGCTGACGTAGAACATTGTTCGCATCCCGGTATCAGATAGTTAACTTTTGTTGTTATTTTGGTTTCAGGCAGTTGTATTAAAATCATATCCAGTGGGGCTGAGTTTGACAGTTTTGGTGAGTTTGGTGGGAGTTGTGGAAGGAGCTATGGTAGCTTTAGCAATGGAATGGAGAAACTCTTGTGCCTGGTCACTTCATTTTGATTTTCAACTCTTGGTCATCCTTTATGCTGTAACTATAAAATCCCACTCCAAACTTCTCTTTTCTTCTTCATTTCTTTTATCCTAGTTTTGATTTTAGTTTTTCAAAATGAAGTTTGTTTACTAAAACACAAGTTAAATAAAAATGAAAAAAAAAAAATTTCCTTCGTTTTTATTTGATTAAATTACTAAAACAAAATGAAGTTAATTTTTTTATTTTTTTTATAATTTGGCAGGGAATAATAATGTCGGGGCTTTCGTACTACGTTCGAGGAGGTGTAATTAAGGAAAAAGGGCCTGTGTTCTTTACAGCATTTTCCCCTCTAAGCACTGTCATTGTTGCAATCATAAGCTCATTCGCACTCTCTGAGGTTTTTTCTTTGGGAAAGTAAGCTTCTCCTCTGCCCTTCTTAAGCACTTCCAAATTTTACTTAAAAAAAGAAATTAGAAAATTCTCTGTGAGAGGCAAAACTTTACTTGCTTTCTGAGGTGAGAGACGTTAAGAATGACTAAATTGTCTTTAAAAGTTTTAGTATAAATTTAGAGTTTGCTATTTTAATTAACTTTACTATTTTTTTTTTTCATTTAATCTTCCTCGTAATTTCCACACGTCTTTGATACTACATTCCTATGCTTGCTGATTGTTCTCAAAGTTTTGGACATAAGATTAATCCATATGTCAACATAGTATCTATTTAACTATGTTTATTTTGAAAGAATACAAGGATGACTTTTGCATGGTCTTATGATGATATGCCTGGACCTAATAAAAGAGCGAACTAATTACTAAGCTAGTGTTGATGCCACCCGGAGGGAAAATATGTGTTCAGGAAGGGATCTTTTGGGAACCACACAAGTATGACTGACTCTATTTGTTTTTGTTATTAATTAAAATGAGTTGAATCGTATACATTGTCGCATTCCATGCCTTCCAAAAGGTGCCTAGAGGACGAGCCACGTTGGAAGGACATCATATCAAAGTTTACAATTAGGACCTCGATAATTGGGGAGCAAAGCCTTTTGACCAAAAATCAATTTCGAATCAAATCTCTTTAGAAAGAAGATCCTTCATTTGTCTTTTTGGTAGTATGCTCTAGTTTACAGTCATTCATGAAATTGTTATCGGTTCTCCTCCCCAAAATCTTATACAAATGGGGTCAGAGAAAGGAAGCGTAGGCTGTTTGAACACCCGAATCAGAAGTTTGATTGAATAGGTCGCTCCAATTGTCACTAGGCTCGAACGTGTAAAGTTACTTCCAAATTCAATTATGAATTGGTGGATGTTGTCAAAAGTGGCCGTGCATGTCATTAGAACATATCACAATATAGAAATTATAGTTCAAGTGGTTAAAACATCTGGAGGATTTGCGAATCCCTCCTGCTTATATTAAAACTTTCCAAGGACCGCCTCATGGTATCCAAGTTGAAAGAGATAAATTGAACAAGTATGGCCGCCCTCTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCTAAGAATTATGGTAGAGCAGTTTATGAATGTCTACGCGGTGGACTTGATTTTACCAAAGATGATGAAAACGTGAATTCCCAACCATTTATGTGTTGGAGAGACCGTTTCCAAGAGGTCATGAGTTCGAATCCCACCCCGTTGTATTAAAAAAAAAAAACTACGAAAAATATTAAGTATTTTATGTATCCCTTTTGAATTACTTGCCTTCTACTTTCTTCACTTTGTCCCACAAAGACTGACCATATTGAGTTTTTGCAGGATTTTGGGAGGAGTGGTCATAATAATAGGACTTTATTTGGTTATGTGGGGCAAAACCAAAGATCCAGCCATTGATGAGCCATCCAATAATTCTTCGCAGAGCCTCCATGTTTCAAACCATCAAGATCATCAACAACTTAAACCAACTAATGAGGTTTCTAAGGCAACAAGGGATGGTTTAGTTTGAATAACGATCATGCGCTGTCCGATAAGATTGTGTTCTTACAGATTTTTACATTTAGAAAAAATTACGCTTGTTTATTAACGATATCTTAGTTAAAATAGGTAAGTATCGCTGT

mRNA sequence

ATGAGCCCCTTTGTGTATTCCCTCTATCGTTTTTTTGTTGCCGCCATTGTTGTAGCTCCATTTGCCTTACACTTTGACAGAAAAAGAAGACCACAGATGACTTGGCCCATCTTTGCCAAGATTCTATTGCTCGGCTCTATGGACTCTTTGGTTATCCCCAACATGTATTTCAGTGCTCTGAAATATGTAACTCCAACTTTCTCCATTGCTATGTCCAACGTTGTTCCTGCCTTTTCCTTCTTCTTCGCTGTCATTTTCAGGATGGAGAAGGTTGATATTAGGAGACTTTCATCCCAGGCAAAGATATTGGGAACTATAGTGGCAGTGGGAGGGGCAATGATAATGACCTTTTTTGGAGGCCCAAACTTGAGATTTCCTTGGACAAAATTAGAACATTTACACAATCACTCTAATTCAACTCCTCCTTCAAACACCCATCAAGATTCTTTGAAGGGTGTCATTTTGACCACAATTGCTTGTATCTGCTCTTCTGTTTCATGCATTCTCCAGGCAGTTGTATTAAAATCATATCCAGTGGGGCTGAGTTTGACAGTTTTGGTGAGTTTGGTGGGAGTTGTGGAAGGAGCTATGGTAGCTTTAGCAATGGAATGGAGAAACTCTTGTGCCTGGTCACTTCATTTTGATTTTCAACTCTTGGTCATCCTTTATGCTGGAATAATAATGTCGGGGCTTTCGTACTACGTTCGAGGAGGTGTAATTAAGGAAAAAGGGCCTGTGTTCTTTACAGCATTTTCCCCTCTAAGCACTGTCATTGTTGCAATCATAAGCTCATTCGCACTCTCTGAGGTTTTTTCTTTGGGAAAGATTTTGGGAGGAGTGGTCATAATAATAGGACTTTATTTGGTTATGTGGGGCAAAACCAAAGATCCAGCCATTGATGAGCCATCCAATAATTCTTCGCAGAGCCTCCATGTTTCAAACCATCAAGATCATCAACAACTTAAACCAACTAATGAGGTTTCTAAGGCAACAAGGGATGGTTTAGTTTGA

Coding sequence (CDS)

ATGAGCCCCTTTGTGTATTCCCTCTATCGTTTTTTTGTTGCCGCCATTGTTGTAGCTCCATTTGCCTTACACTTTGACAGAAAAAGAAGACCACAGATGACTTGGCCCATCTTTGCCAAGATTCTATTGCTCGGCTCTATGGACTCTTTGGTTATCCCCAACATGTATTTCAGTGCTCTGAAATATGTAACTCCAACTTTCTCCATTGCTATGTCCAACGTTGTTCCTGCCTTTTCCTTCTTCTTCGCTGTCATTTTCAGGATGGAGAAGGTTGATATTAGGAGACTTTCATCCCAGGCAAAGATATTGGGAACTATAGTGGCAGTGGGAGGGGCAATGATAATGACCTTTTTTGGAGGCCCAAACTTGAGATTTCCTTGGACAAAATTAGAACATTTACACAATCACTCTAATTCAACTCCTCCTTCAAACACCCATCAAGATTCTTTGAAGGGTGTCATTTTGACCACAATTGCTTGTATCTGCTCTTCTGTTTCATGCATTCTCCAGGCAGTTGTATTAAAATCATATCCAGTGGGGCTGAGTTTGACAGTTTTGGTGAGTTTGGTGGGAGTTGTGGAAGGAGCTATGGTAGCTTTAGCAATGGAATGGAGAAACTCTTGTGCCTGGTCACTTCATTTTGATTTTCAACTCTTGGTCATCCTTTATGCTGGAATAATAATGTCGGGGCTTTCGTACTACGTTCGAGGAGGTGTAATTAAGGAAAAAGGGCCTGTGTTCTTTACAGCATTTTCCCCTCTAAGCACTGTCATTGTTGCAATCATAAGCTCATTCGCACTCTCTGAGGTTTTTTCTTTGGGAAAGATTTTGGGAGGAGTGGTCATAATAATAGGACTTTATTTGGTTATGTGGGGCAAAACCAAAGATCCAGCCATTGATGAGCCATCCAATAATTCTTCGCAGAGCCTCCATGTTTCAAACCATCAAGATCATCAACAACTTAAACCAACTAATGAGGTTTCTAAGGCAACAAGGGATGGTTTAGTTTGA

Protein sequence

MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSALKYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGGPNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVGLSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVIKEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAIDEPSNNSSQSLHVSNHQDHQQLKPTNEVSKATRDGLV

Homology

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BLAST of MC04g_new0575 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 3.3e-77
Identity = 157/307 (51.14%), Postives = 211/307 (68.73%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSP V + YR  VA I +APFA   DRK RP+MT  IF KILLLG ++  +  N+Y++ +
Sbjct: 34  MSPHVLASYRHIVATIFIAPFAYFLDRKIRPKMTLSIFFKILLLGLLEPTIDQNLYYTGM 93

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           KY + TF+ AM+NV+PAF+F  A IFR+EKV+++++ SQAKILGTIV VGGAM+MT   G
Sbjct: 94  KYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIHSQAKILGTIVTVGGAMLMTVVKG 153

Query: 121 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 180
           P +  PW     +H  S++T      QD  KG  L  I CIC +    LQA+ LKSYPV 
Sbjct: 154 PLIPLPWANPHDIHQDSSNT---GVKQDLTKGASLIAIGCICWAGFINLQAITLKSYPVE 213

Query: 181 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 240
           LSLT  +  +G +E  +VAL +E  N  AW++H D +LL  +Y G+I SG+ YYV+G ++
Sbjct: 214 LSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDSKLLAAVYGGVICSGIGYYVQGVIM 273

Query: 241 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID 300
           K +GPVF TAF+PLS VIVAI+ S  L+EV  LG+ILG +VI++GLY V+WGK+K    D
Sbjct: 274 KTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRILGAIVIVLGLYSVLWGKSK----D 333

Query: 301 EPSNNSS 308
           EPS++ S
Sbjct: 334 EPSSSFS 333

[Close]

BLAST of MC04g_new0575 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.3e-68
Identity = 145/296 (48.99%), Postives = 196/296 (66.22%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MS +V  +YR  VA IV+APFA +FD+K RP+MT  IF KI LLG ++ ++  N+Y+  +
Sbjct: 41  MSNYVLVVYRHAVATIVMAPFAFYFDKKVRPKMTLMIFFKISLLGLLEPVIDQNLYYLGM 100

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           KY T TF+ AM NV+PA +F  A IF +E+V +R + S  K++GT+  VGGAMIMT   G
Sbjct: 101 KYTTATFATAMYNVLPAITFVLAYIFGLERVKLRCIRSTGKVVGTLATVGGAMIMTLVKG 160

Query: 121 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 180
           P L   WTK    HN    T  ++ H  ++KG +L TI C   +   ILQA+ L++YP  
Sbjct: 161 PVLDLFWTKGVSAHN----TAGTDIH-SAIKGAVLVTIGCFSYACFMILQAITLRTYPAE 220

Query: 181 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 240
           LSLT  + L+G +EG  VAL ME  N  AW++ +D +LL   Y+GI+ S L+YYV G V+
Sbjct: 221 LSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGWDTKLLTATYSGIVCSALAYYVGGVVM 280

Query: 241 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKD 297
           K +GPVF TAFSPL  +IVAI+S+   +E   LG++LG VVI  GLYLV+WGK KD
Sbjct: 281 KTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLGRVLGAVVICAGLYLVIWGKGKD 331

[Close]

BLAST of MC04g_new0575 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-65
Identity = 138/301 (45.85%), Postives = 199/301 (66.11%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MS +V  +YR  +A  V+APFA  F+RK +P++T+ IF ++ +LG +  ++  N Y+  L
Sbjct: 44  MSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGL 103

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           KY +PTFS AMSN++PA +F  AV+FRME +D+++L  QAKI GT+V V GAM+MT + G
Sbjct: 104 KYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKG 163

Query: 121 PNLRFPWTKLEHLH--NHSNSTPPSNTHQDS--LKGVILTTIACICSSVSCILQAVVLKS 180
           P +   WTK  H+   +H+N+T   N+  D   LKG IL   A +  +   +LQA +LK+
Sbjct: 164 PIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKT 223

Query: 181 YPV-GLSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYV 240
           Y    LSLT L+  +G ++   V   ME  N  AW + +D  LL   Y+GI+ S +SYYV
Sbjct: 224 YAKHQLSLTTLICFIGTLQAVAVTFVME-HNPSAWRIGWDMNLLAAAYSGIVASSISYYV 283

Query: 241 RGGVIKEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTK 297
           +G V+K++GPVF TAFSPL  VIVA++ SF L+E   LG ++G V+I+IGLY V+WGK K
Sbjct: 284 QGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQK 343

[Close]

BLAST of MC04g_new0575 vs. ExPASy Swiss-Prot
Match: Q9FNA5 (WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.4e-59
Identity = 128/299 (42.81%), Postives = 189/299 (63.21%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSP V   YR  VA+ ++ PFAL  +R  RP++T+ I  +I +L   + +V  N+Y+S +
Sbjct: 34  MSPHVLVAYRMAVASALITPFALILERNTRPKLTFKILLQIAILSLFEPVVEQNLYYSGM 93

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           K  T TF+ A+ N +PA +F  A +F++EKV I R  SQAK++GT+VA+GGAM+MTF  G
Sbjct: 94  KLTTATFTSALCNALPAMTFIMACVFKLEKVTIERRHSQAKLVGTMVAIGGAMLMTFVKG 153

Query: 121 PNLRFPWTKLE---HLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSY 180
             +  PWT      + H H+   P      D  +G I+   +C   S   ILQA +L  Y
Sbjct: 154 NVIELPWTSNSRGLNGHTHAMRIP---KQADIARGSIMLVASCFSWSCYIILQAKILAQY 213

Query: 181 PVGLSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRG 240
              LSLT L+ ++G++E  ++ L  E +N   W ++ D  LL  +Y G++ SGL+YYV G
Sbjct: 214 KAELSLTALMCIMGMLEATVMGLIWERKNMSVWKINPDVTLLASIYGGLV-SGLAYYVIG 273

Query: 241 GVIKEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKD 297
              KE+GPVF +AF+PLS V+VAI+S+F   E   +G+++G VVI+IG+YLV+WGK+KD
Sbjct: 274 WASKERGPVFVSAFNPLSMVLVAILSTFVFLEKVYVGRVIGSVVIVIGIYLVLWGKSKD 328

[Close]

BLAST of MC04g_new0575 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 9.2e-59
Identity = 131/338 (38.76%), Postives = 212/338 (62.72%), Query Frame = 0


Query: 4   FVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSALKYV 63
           +V  +YR  VAA+V+APFAL F+RK RP+MT  +  KI+ LG ++ ++     +  +   
Sbjct: 42  YVVIVYRNLVAALVLAPFALIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMT 101

Query: 64  TPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGGPNL 123
           + T++ A+ N++P+ +F  A I RMEKV+I  + S+AKI+GT+V +GGA++MT + GP +
Sbjct: 102 SATYTSAIMNILPSVTFIIAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLI 161

Query: 124 RFPWT--KLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVGL 183
             PW+   ++  + H+N+   S  H + + G +L  + C+  S   +LQ++ +K+YP  L
Sbjct: 162 PLPWSNPNMDQQNGHTNN---SQDHNNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADL 221

Query: 184 SLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVIK 243
           SL+ L+ L G V+   VAL +E R+   W++ +D +L   LY GI+ SG++YYV+G V+K
Sbjct: 222 SLSALICLAGAVQSFAVALVVE-RHPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMK 281

Query: 244 EKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKD---PA 303
            +GPVF TAF+PL  ++VA+I+SF L E    G ++GG VI  GLY+V+WGK KD     
Sbjct: 282 TRGPVFVTAFNPLCMILVALIASFILHEQIHFGCVIGGAVIAAGLYMVVWGKGKDYEVSG 341

Query: 304 IDEPSNNSSQSLHVS--NHQDHQQLKPTNEVSKATRDG 335
           +D    NS Q L ++  +  D++ +   ++ S  T  G
Sbjct: 342 LDILEKNSLQELPITTKSEDDNKLVSSISDNSNVTIPG 375

[Close]

BLAST of MC04g_new0575 vs. NCBI nr
Match: XP_022133584.1 (WAT1-related protein At2g39510-like [Momordica charantia])

HSP 1 Score: 639 bits (1647), Expect = 8.65e-230
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL
Sbjct: 40  MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 99

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG
Sbjct: 100 KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 159

Query: 121 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 180
           PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG
Sbjct: 160 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 219

Query: 181 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 240
           LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI
Sbjct: 220 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 279

Query: 241 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID 300
           KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID
Sbjct: 280 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID 339

Query: 301 EPSNNSSQSLHVSNHQDHQQLKPTNEVSKATRDGLV 336
           EPSNNSSQSLHVSNHQDHQQLKPTNEVSKATRDGLV
Sbjct: 340 EPSNNSSQSLHVSNHQDHQQLKPTNEVSKATRDGLV 375

[Close]

BLAST of MC04g_new0575 vs. NCBI nr
Match: XP_022133583.1 (WAT1-related protein At2g39510-like [Momordica charantia])

HSP 1 Score: 488 bits (1256), Expect = 3.25e-170
Identity = 261/335 (77.91%), Postives = 293/335 (87.46%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSPFVYSLYR+FV+AIVVAPFAL  DRKRRPQMTWPIFAKILLLGSMD +V+PNMYFS L
Sbjct: 41  MSPFVYSLYRYFVSAIVVAPFALLLDRKRRPQMTWPIFAKILLLGSMDCVVLPNMYFSGL 100

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           KYVTPTFSIAMSN  PA SFFFAV  RMEKVDIRR SSQ KILGT+VAVGGAMIMTFF G
Sbjct: 101 KYVTPTFSIAMSNAAPALSFFFAVTLRMEKVDIRRFSSQVKILGTVVAVGGAMIMTFFRG 160

Query: 121 PNLRFPWTKLEHLHNH---SNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSY 180
           PNLRFPWTKL+  HN+   +NST PSNTHQDS KGVIL TI+C+CSS+SCILQAVVLKSY
Sbjct: 161 PNLRFPWTKLQDSHNNHHSNNSTSPSNTHQDSFKGVILVTISCLCSSLSCILQAVVLKSY 220

Query: 181 PVGLSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRG 240
           PVGLS+TVLVSLVGVV GA+ ALAMEW NSCAW++HFDFQLL ILYAG I+SGLSYYV+G
Sbjct: 221 PVGLSVTVLVSLVGVVGGAVAALAMEWNNSCAWAIHFDFQLLAILYAGTIISGLSYYVQG 280

Query: 241 GVIKEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDP 300
            V++EKGPVF TAF PL TV+VAIISSFA+SE+ SL K+LGGVVI++GLYLVMWGKTKD 
Sbjct: 281 VVMEEKGPVFLTAFVPLGTVMVAIISSFAVSEILSLEKVLGGVVIVVGLYLVMWGKTKDS 340

Query: 301 AI-DEPSNNSSQSLHVSNH-QDHQQLKPTNEVSKA 330
           AI D+ +NNSSQS   S+H QD Q+LK  + V++A
Sbjct: 341 AIVDKAANNSSQSFEASDHHQDDQRLKAADGVARA 375

[Close]

BLAST of MC04g_new0575 vs. NCBI nr
Match: XP_022961609.1 (WAT1-related protein At2g39510-like [Cucurbita moschata])

HSP 1 Score: 403 bits (1036), Expect = 3.80e-137
Identity = 216/304 (71.05%), Postives = 255/304 (83.88%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSP VYSLYR+ VAAIVVAPFAL FDR+RRP+M+W +FAKILLLGSMDS+VIPN YF+ L
Sbjct: 39  MSPQVYSLYRYCVAAIVVAPFALLFDRRRRPEMSWCMFAKILLLGSMDSVVIPNTYFTGL 98

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           K+V+PTFSIAMSN VPA SFFFA IFRMEKVDIRR+SS AKI+GT V VGGAM M F   
Sbjct: 99  KFVSPTFSIAMSNAVPALSFFFAWIFRMEKVDIRRVSSWAKIIGTAVTVGGAMTMIFVRH 158

Query: 121 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 180
           P LRF WTK     +HS++   +N      KGVIL TI+C+ SS+SC+LQA+VLKSYPVG
Sbjct: 159 PTLRFGWTKGADSQHHSSTA--ANNQDYRFKGVILVTISCVSSSLSCVLQAIVLKSYPVG 218

Query: 181 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 240
           LSLTVLVSLVGVVEG+++ALAMEW N  AWS+HFDFQLL ILYAGI++SGLSYYV+G V+
Sbjct: 219 LSLTVLVSLVGVVEGSVIALAMEWNNPAAWSIHFDFQLLAILYAGILISGLSYYVQGVVM 278

Query: 241 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID 300
           + KG VF TAF PLSTVIVAIISSFA+SE+ SLGKI+G V+II+GLYL++W KTKD A+D
Sbjct: 279 EAKGAVFLTAFFPLSTVIVAIISSFAVSEILSLGKIVGAVIIILGLYLILWAKTKDQALD 338

Query: 301 EPSN 304
           + +N
Sbjct: 339 KAAN 340

[Close]

BLAST of MC04g_new0575 vs. NCBI nr
Match: KAG6602357.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 400 bits (1027), Expect = 8.84e-136
Identity = 220/336 (65.48%), Postives = 263/336 (78.27%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSP VYSLYR  VAAIVVAPFAL FDR+RRP+M+W +F KILLLGSMDS+VIPN YF+ L
Sbjct: 39  MSPQVYSLYRCCVAAIVVAPFALLFDRRRRPEMSWCMFGKILLLGSMDSVVIPNTYFTGL 98

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           K+V+PTFSIAMSN VPA SFFFA IFRMEKVDIRR+SS AKILGT V VGGAM M F   
Sbjct: 99  KFVSPTFSIAMSNAVPALSFFFAWIFRMEKVDIRRVSSWAKILGTAVTVGGAMTMIFVRH 158

Query: 121 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 180
           P LRF WTK     +HS++   +N      KGVIL TI+C+ SS+SC+LQA+VLKSYPVG
Sbjct: 159 PTLRFGWTKGADSQHHSSTA--ANNQDYRFKGVILVTISCVSSSLSCVLQAIVLKSYPVG 218

Query: 181 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 240
           LSLTVLVSLVGVVEG+++ALAMEW N  AWS+HFDFQLL ILYAGI++SGLSYYV+G V+
Sbjct: 219 LSLTVLVSLVGVVEGSVIALAMEWNNPAAWSIHFDFQLLAILYAGILISGLSYYVQGVVM 278

Query: 241 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID 300
           + KG VF TAF PLSTVIVAIISSFA+SE+ SLGKI+G ++II+GLYL++W KTKD A+D
Sbjct: 279 EAKGAVFLTAFFPLSTVIVAIISSFAVSEILSLGKIVGALIIILGLYLILWAKTKDQALD 338

Query: 301 EPSNNSSQSLHVSNHQDHQQLKPTNEVSKATRDGLV 336
           + +N                    N+V+++T +G V
Sbjct: 339 KAAN------------------AVNDVARSTTNGSV 354

[Close]

BLAST of MC04g_new0575 vs. NCBI nr
Match: XP_023534785.1 (WAT1-related protein At2g39510-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399 bits (1024), Expect = 2.52e-135
Identity = 219/336 (65.18%), Postives = 263/336 (78.27%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSP VYSLYR+ VAAIVVAPFAL FDR+RRP+M+W +F KILLLGSMDS+VIPN YF+ L
Sbjct: 39  MSPQVYSLYRYCVAAIVVAPFALLFDRRRRPEMSWCMFGKILLLGSMDSVVIPNTYFTGL 98

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           K+V+PTFSIAMSN VPA SFFFA IFRMEKVDIRR+SS AKILGT V VGGAM M F   
Sbjct: 99  KFVSPTFSIAMSNAVPALSFFFAWIFRMEKVDIRRVSSWAKILGTAVTVGGAMTMIFVRH 158

Query: 121 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 180
           P L F WTK     +HS++   +N      KGVIL TI+C+ SS+SC+LQA+VLKSYPVG
Sbjct: 159 PTLSFGWTKGADSQHHSSTD--ANNQDYRFKGVILVTISCVSSSLSCVLQAIVLKSYPVG 218

Query: 181 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 240
           LSLTVLVSLVGVVEG+++ALAMEW N  AWS+HFDFQLL ILYAGI++SGLSYYV+G V+
Sbjct: 219 LSLTVLVSLVGVVEGSVIALAMEWNNPAAWSIHFDFQLLAILYAGILISGLSYYVQGVVM 278

Query: 241 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID 300
           + KG VF TAF PLSTVIVAIISSFA+SE+ SLGKI+G ++II+GLYL++W KTKD A+D
Sbjct: 279 EAKGAVFLTAFFPLSTVIVAIISSFAVSEILSLGKIVGALIIILGLYLILWAKTKDRALD 338

Query: 301 EPSNNSSQSLHVSNHQDHQQLKPTNEVSKATRDGLV 336
           + +N                    N+V+++T +G V
Sbjct: 339 KAAN------------------AVNDVARSTTNGSV 354

[Close]

BLAST of MC04g_new0575 vs. ExPASy TrEMBL
Match: A0A6J1BWE1 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111006125 PE=3 SV=1)

HSP 1 Score: 639 bits (1647), Expect = 4.19e-230
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL
Sbjct: 40  MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 99

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG
Sbjct: 100 KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 159

Query: 121 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 180
           PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG
Sbjct: 160 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 219

Query: 181 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 240
           LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI
Sbjct: 220 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 279

Query: 241 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID 300
           KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID
Sbjct: 280 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID 339

Query: 301 EPSNNSSQSLHVSNHQDHQQLKPTNEVSKATRDGLV 336
           EPSNNSSQSLHVSNHQDHQQLKPTNEVSKATRDGLV
Sbjct: 340 EPSNNSSQSLHVSNHQDHQQLKPTNEVSKATRDGLV 375

[Close]

BLAST of MC04g_new0575 vs. ExPASy TrEMBL
Match: A0A6J1BZJ0 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111006124 PE=3 SV=1)

HSP 1 Score: 488 bits (1256), Expect = 1.57e-170
Identity = 261/335 (77.91%), Postives = 293/335 (87.46%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSPFVYSLYR+FV+AIVVAPFAL  DRKRRPQMTWPIFAKILLLGSMD +V+PNMYFS L
Sbjct: 41  MSPFVYSLYRYFVSAIVVAPFALLLDRKRRPQMTWPIFAKILLLGSMDCVVLPNMYFSGL 100

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           KYVTPTFSIAMSN  PA SFFFAV  RMEKVDIRR SSQ KILGT+VAVGGAMIMTFF G
Sbjct: 101 KYVTPTFSIAMSNAAPALSFFFAVTLRMEKVDIRRFSSQVKILGTVVAVGGAMIMTFFRG 160

Query: 121 PNLRFPWTKLEHLHNH---SNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSY 180
           PNLRFPWTKL+  HN+   +NST PSNTHQDS KGVIL TI+C+CSS+SCILQAVVLKSY
Sbjct: 161 PNLRFPWTKLQDSHNNHHSNNSTSPSNTHQDSFKGVILVTISCLCSSLSCILQAVVLKSY 220

Query: 181 PVGLSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRG 240
           PVGLS+TVLVSLVGVV GA+ ALAMEW NSCAW++HFDFQLL ILYAG I+SGLSYYV+G
Sbjct: 221 PVGLSVTVLVSLVGVVGGAVAALAMEWNNSCAWAIHFDFQLLAILYAGTIISGLSYYVQG 280

Query: 241 GVIKEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDP 300
            V++EKGPVF TAF PL TV+VAIISSFA+SE+ SL K+LGGVVI++GLYLVMWGKTKD 
Sbjct: 281 VVMEEKGPVFLTAFVPLGTVMVAIISSFAVSEILSLEKVLGGVVIVVGLYLVMWGKTKDS 340

Query: 301 AI-DEPSNNSSQSLHVSNH-QDHQQLKPTNEVSKA 330
           AI D+ +NNSSQS   S+H QD Q+LK  + V++A
Sbjct: 341 AIVDKAANNSSQSFEASDHHQDDQRLKAADGVARA 375

[Close]

BLAST of MC04g_new0575 vs. ExPASy TrEMBL
Match: A0A6J1HEK0 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111462246 PE=3 SV=1)

HSP 1 Score: 403 bits (1036), Expect = 1.84e-137
Identity = 216/304 (71.05%), Postives = 255/304 (83.88%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSP VYSLYR+ VAAIVVAPFAL FDR+RRP+M+W +FAKILLLGSMDS+VIPN YF+ L
Sbjct: 39  MSPQVYSLYRYCVAAIVVAPFALLFDRRRRPEMSWCMFAKILLLGSMDSVVIPNTYFTGL 98

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           K+V+PTFSIAMSN VPA SFFFA IFRMEKVDIRR+SS AKI+GT V VGGAM M F   
Sbjct: 99  KFVSPTFSIAMSNAVPALSFFFAWIFRMEKVDIRRVSSWAKIIGTAVTVGGAMTMIFVRH 158

Query: 121 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 180
           P LRF WTK     +HS++   +N      KGVIL TI+C+ SS+SC+LQA+VLKSYPVG
Sbjct: 159 PTLRFGWTKGADSQHHSSTA--ANNQDYRFKGVILVTISCVSSSLSCVLQAIVLKSYPVG 218

Query: 181 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 240
           LSLTVLVSLVGVVEG+++ALAMEW N  AWS+HFDFQLL ILYAGI++SGLSYYV+G V+
Sbjct: 219 LSLTVLVSLVGVVEGSVIALAMEWNNPAAWSIHFDFQLLAILYAGILISGLSYYVQGVVM 278

Query: 241 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID 300
           + KG VF TAF PLSTVIVAIISSFA+SE+ SLGKI+G V+II+GLYL++W KTKD A+D
Sbjct: 279 EAKGAVFLTAFFPLSTVIVAIISSFAVSEILSLGKIVGAVIIILGLYLILWAKTKDQALD 338

Query: 301 EPSN 304
           + +N
Sbjct: 339 KAAN 340

[Close]

BLAST of MC04g_new0575 vs. ExPASy TrEMBL
Match: A0A1S3CJT7 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103501643 PE=3 SV=1)

HSP 1 Score: 390 bits (1003), Expect = 2.09e-132
Identity = 205/302 (67.88%), Postives = 253/302 (83.77%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFA-LHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSA 60
           MSP VYSLYR+FVA+IVVAPF+ L + +++RP M+W  FAKILLLGS++S+VI N YF+ 
Sbjct: 40  MSPQVYSLYRYFVASIVVAPFSFLSYRKRQRPHMSWCTFAKILLLGSVESVVITNTYFTG 99

Query: 61  LKYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFG 120
           LKYVTPTFSIAMSN VPA SFFFA +FRMEK+D+ R SSQAKILGT V+VGGAMIMTF  
Sbjct: 100 LKYVTPTFSIAMSNAVPALSFFFASLFRMEKIDVIRFSSQAKILGTAVSVGGAMIMTFVE 159

Query: 121 GPNLRFPWTK-LEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYP 180
           GP LRFPWT    +LH + +++  +  ++DS KG+IL TI+C+C+SVSCILQA+VLKSYP
Sbjct: 160 GPKLRFPWTNGHNNLHCYPSTSSINVNNEDSFKGIILVTISCLCASVSCILQAIVLKSYP 219

Query: 181 VGLSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGG 240
           +GL +T +V +VGVVEGA+VALAMEW N   WS+HFDFQLL ILYAGII+SG SYY++G 
Sbjct: 220 MGLIVTFMVCIVGVVEGAVVALAMEWNNPSVWSIHFDFQLLAILYAGIIISGFSYYIQGV 279

Query: 241 VIKEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPA 300
           V++EKGPVF TAF PL+TV+VAIISSFA+SE+ SLGK++G +VII GLYL +WGKTKD  
Sbjct: 280 VMEEKGPVFLTAFFPLTTVMVAIISSFAISEILSLGKVVGAMVIIAGLYLFLWGKTKDQV 339

[Close]

BLAST of MC04g_new0575 vs. ExPASy TrEMBL
Match: A0A0A0KNS0 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_5G506320 PE=3 SV=1)

HSP 1 Score: 385 bits (990), Expect = 1.83e-130
Identity = 213/304 (70.07%), Postives = 248/304 (81.58%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFA-LHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSA 60
           MSP VYSLYR+FVA+IVVAPF  L + +  RP+MTW I AKILLLG+M+S+VI N YF+ 
Sbjct: 40  MSPQVYSLYRYFVASIVVAPFCFLSYRKGPRPRMTWCILAKILLLGTMESVVITNTYFTG 99

Query: 61  LKYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFG 120
           LKYVTPTFS AMSN +PA SFFFA IF MEKVDIRR SSQ KI+GT V VGGAMIMTF  
Sbjct: 100 LKYVTPTFSTAMSNCIPALSFFFAWIFGMEKVDIRRFSSQVKIIGTAVTVGGAMIMTFVE 159

Query: 121 GPNLRFPWTKLEH--LHNHSNSTPPSNTH-QDSLKGVILTTIACICSSVSCILQAVVLKS 180
           GP  RFPWT  EH  LHNHS STPPSN + QDS KGVIL TIA + +SVSCI+QA+VLKS
Sbjct: 160 GPKFRFPWTN-EHNSLHNHS-STPPSNVNNQDSFKGVILVTIAILGASVSCIIQAIVLKS 219

Query: 181 YPVGLSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVR 240
           YP+GL +T +V +VGVVEG +VALA EW N   WS+HFDFQLL  LYAGI+MSG SY+++
Sbjct: 220 YPLGLVVTFMVCIVGVVEGTVVALAKEWNNPPVWSIHFDFQLLAFLYAGIMMSGFSYFIQ 279

Query: 241 GGVIKEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKD 300
           G V++ KGPVF T F PLST+IVAIISSFA+SEV SLGK++G +VIIIGLYLV+WGKTKD
Sbjct: 280 GVVLEAKGPVFLTIFFPLSTIIVAIISSFAISEVLSLGKVMGALVIIIGLYLVLWGKTKD 339

[Close]

BLAST of MC04g_new0575 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 290.0 bits (741), Expect = 2.4e-78
Identity = 157/307 (51.14%), Postives = 211/307 (68.73%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSP V + YR  VA I +APFA   DRK RP+MT  IF KILLLG ++  +  N+Y++ +
Sbjct: 34  MSPHVLASYRHIVATIFIAPFAYFLDRKIRPKMTLSIFFKILLLGLLEPTIDQNLYYTGM 93

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           KY + TF+ AM+NV+PAF+F  A IFR+EKV+++++ SQAKILGTIV VGGAM+MT   G
Sbjct: 94  KYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIHSQAKILGTIVTVGGAMLMTVVKG 153

Query: 121 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 180
           P +  PW     +H  S++T      QD  KG  L  I CIC +    LQA+ LKSYPV 
Sbjct: 154 PLIPLPWANPHDIHQDSSNT---GVKQDLTKGASLIAIGCICWAGFINLQAITLKSYPVE 213

Query: 181 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 240
           LSLT  +  +G +E  +VAL +E  N  AW++H D +LL  +Y G+I SG+ YYV+G ++
Sbjct: 214 LSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDSKLLAAVYGGVICSGIGYYVQGVIM 273

Query: 241 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKDPAID 300
           K +GPVF TAF+PLS VIVAI+ S  L+EV  LG+ILG +VI++GLY V+WGK+K    D
Sbjct: 274 KTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRILGAIVIVLGLYSVLWGKSK----D 333

Query: 301 EPSNNSS 308
           EPS++ S
Sbjct: 334 EPSSSFS 333

[Close]

BLAST of MC04g_new0575 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 259.2 bits (661), Expect = 4.5e-69
Identity = 145/296 (48.99%), Postives = 196/296 (66.22%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MS +V  +YR  VA IV+APFA +FD+K RP+MT  IF KI LLG ++ ++  N+Y+  +
Sbjct: 41  MSNYVLVVYRHAVATIVMAPFAFYFDKKVRPKMTLMIFFKISLLGLLEPVIDQNLYYLGM 100

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           KY T TF+ AM NV+PA +F  A IF +E+V +R + S  K++GT+  VGGAMIMT   G
Sbjct: 101 KYTTATFATAMYNVLPAITFVLAYIFGLERVKLRCIRSTGKVVGTLATVGGAMIMTLVKG 160

Query: 121 PNLRFPWTKLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVG 180
           P L   WTK    HN    T  ++ H  ++KG +L TI C   +   ILQA+ L++YP  
Sbjct: 161 PVLDLFWTKGVSAHN----TAGTDIH-SAIKGAVLVTIGCFSYACFMILQAITLRTYPAE 220

Query: 181 LSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVI 240
           LSLT  + L+G +EG  VAL ME  N  AW++ +D +LL   Y+GI+ S L+YYV G V+
Sbjct: 221 LSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGWDTKLLTATYSGIVCSALAYYVGGVVM 280

Query: 241 KEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKD 297
           K +GPVF TAFSPL  +IVAI+S+   +E   LG++LG VVI  GLYLV+WGK KD
Sbjct: 281 KTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLGRVLGAVVICAGLYLVIWGKGKD 331

[Close]

BLAST of MC04g_new0575 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 251.9 bits (642), Expect = 7.2e-67
Identity = 138/301 (45.85%), Postives = 199/301 (66.11%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MS +V  +YR  +A  V+APFA  F+RK +P++T+ IF ++ +LG +  ++  N Y+  L
Sbjct: 44  MSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGL 103

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           KY +PTFS AMSN++PA +F  AV+FRME +D+++L  QAKI GT+V V GAM+MT + G
Sbjct: 104 KYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKG 163

Query: 121 PNLRFPWTKLEHLH--NHSNSTPPSNTHQDS--LKGVILTTIACICSSVSCILQAVVLKS 180
           P +   WTK  H+   +H+N+T   N+  D   LKG IL   A +  +   +LQA +LK+
Sbjct: 164 PIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKT 223

Query: 181 YPV-GLSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYV 240
           Y    LSLT L+  +G ++   V   ME  N  AW + +D  LL   Y+GI+ S +SYYV
Sbjct: 224 YAKHQLSLTTLICFIGTLQAVAVTFVME-HNPSAWRIGWDMNLLAAAYSGIVASSISYYV 283

Query: 241 RGGVIKEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTK 297
           +G V+K++GPVF TAFSPL  VIVA++ SF L+E   LG ++G V+I+IGLY V+WGK K
Sbjct: 284 QGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQK 343

[Close]

BLAST of MC04g_new0575 vs. TAIR 10
Match: AT5G13670.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 231.5 bits (589), Expect = 1.0e-60
Identity = 128/299 (42.81%), Postives = 189/299 (63.21%), Query Frame = 0


Query: 1   MSPFVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSAL 60
           MSP V   YR  VA+ ++ PFAL  +R  RP++T+ I  +I +L   + +V  N+Y+S +
Sbjct: 34  MSPHVLVAYRMAVASALITPFALILERNTRPKLTFKILLQIAILSLFEPVVEQNLYYSGM 93

Query: 61  KYVTPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGG 120
           K  T TF+ A+ N +PA +F  A +F++EKV I R  SQAK++GT+VA+GGAM+MTF  G
Sbjct: 94  KLTTATFTSALCNALPAMTFIMACVFKLEKVTIERRHSQAKLVGTMVAIGGAMLMTFVKG 153

Query: 121 PNLRFPWTKLE---HLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSY 180
             +  PWT      + H H+   P      D  +G I+   +C   S   ILQA +L  Y
Sbjct: 154 NVIELPWTSNSRGLNGHTHAMRIP---KQADIARGSIMLVASCFSWSCYIILQAKILAQY 213

Query: 181 PVGLSLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRG 240
              LSLT L+ ++G++E  ++ L  E +N   W ++ D  LL  +Y G++ SGL+YYV G
Sbjct: 214 KAELSLTALMCIMGMLEATVMGLIWERKNMSVWKINPDVTLLASIYGGLV-SGLAYYVIG 273

Query: 241 GVIKEKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKD 297
              KE+GPVF +AF+PLS V+VAI+S+F   E   +G+++G VVI+IG+YLV+WGK+KD
Sbjct: 274 WASKERGPVFVSAFNPLSMVLVAILSTFVFLEKVYVGRVIGSVVIVIGIYLVLWGKSKD 328

[Close]

BLAST of MC04g_new0575 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 228.8 bits (582), Expect = 6.5e-60
Identity = 131/338 (38.76%), Postives = 212/338 (62.72%), Query Frame = 0


Query: 4   FVYSLYRFFVAAIVVAPFALHFDRKRRPQMTWPIFAKILLLGSMDSLVIPNMYFSALKYV 63
           +V  +YR  VAA+V+APFAL F+RK RP+MT  +  KI+ LG ++ ++     +  +   
Sbjct: 42  YVVIVYRNLVAALVLAPFALIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMT 101

Query: 64  TPTFSIAMSNVVPAFSFFFAVIFRMEKVDIRRLSSQAKILGTIVAVGGAMIMTFFGGPNL 123
           + T++ A+ N++P+ +F  A I RMEKV+I  + S+AKI+GT+V +GGA++MT + GP +
Sbjct: 102 SATYTSAIMNILPSVTFIIAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLI 161

Query: 124 RFPWT--KLEHLHNHSNSTPPSNTHQDSLKGVILTTIACICSSVSCILQAVVLKSYPVGL 183
             PW+   ++  + H+N+   S  H + + G +L  + C+  S   +LQ++ +K+YP  L
Sbjct: 162 PLPWSNPNMDQQNGHTNN---SQDHNNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADL 221

Query: 184 SLTVLVSLVGVVEGAMVALAMEWRNSCAWSLHFDFQLLVILYAGIIMSGLSYYVRGGVIK 243
           SL+ L+ L G V+   VAL +E R+   W++ +D +L   LY GI+ SG++YYV+G V+K
Sbjct: 222 SLSALICLAGAVQSFAVALVVE-RHPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMK 281

Query: 244 EKGPVFFTAFSPLSTVIVAIISSFALSEVFSLGKILGGVVIIIGLYLVMWGKTKD---PA 303
            +GPVF TAF+PL  ++VA+I+SF L E    G ++GG VI  GLY+V+WGK KD     
Sbjct: 282 TRGPVFVTAFNPLCMILVALIASFILHEQIHFGCVIGGAVIAAGLYMVVWGKGKDYEVSG 341

Query: 304 IDEPSNNSSQSLHVS--NHQDHQQLKPTNEVSKATRDG 335
           +D    NS Q L ++  +  D++ +   ++ S  T  G
Sbjct: 342 LDILEKNSLQELPITTKSEDDNKLVSSISDNSNVTIPG 375

The following BLAST results are available for this feature:

BLAST of MC04g_new0575 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80638	3.3e-77	51.14	WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2...	[more]
Q9ZUS1	6.3e-68	48.99	WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2...	[more]
Q9FL41	1.0e-65	45.85	WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2...	[more]
Q9FNA5	1.4e-59	42.81	WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2...	[more]
Q9SUF1	9.2e-59	38.76	WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2...	[more]

BLAST of MC04g_new0575 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133584.1	8.65e-230	100.00	WAT1-related protein At2g39510-like [Momordica charantia]	[more]
XP_022133583.1	3.25e-170	77.91	WAT1-related protein At2g39510-like [Momordica charantia]	[more]
XP_022961609.1	3.80e-137	71.05	WAT1-related protein At2g39510-like [Cucurbita moschata]	[more]
KAG6602357.1	8.84e-136	65.48	WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023534785.1	2.52e-135	65.18	WAT1-related protein At2g39510-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC04g_new0575 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BWE1	4.19e-230	100.00	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111006125 PE=3 SV=1	[more]
A0A6J1BZJ0	1.57e-170	77.91	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111006124 PE=3 SV=1	[more]
A0A6J1HEK0	1.84e-137	71.05	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111462246 PE=3 SV=1	[more]
A0A1S3CJT7	2.09e-132	67.88	WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103501643 PE=3 SV=1	[more]
A0A0A0KNS0	1.83e-130	70.07	WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_5G506320 PE=3 SV=1	[more]

BLAST of MC04g_new0575 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39510.1	2.4e-78	51.14	nodulin MtN21 /EamA-like transporter family protein	[more]
AT2G37460.1	4.5e-69	48.99	nodulin MtN21 /EamA-like transporter family protein	[more]
AT5G07050.1	7.2e-67	45.85	nodulin MtN21 /EamA-like transporter family protein	[more]
AT5G13670.1	1.0e-60	42.81	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G08290.1	6.5e-60	38.76	nodulin MtN21 /EamA-like transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 1..115 e-value: 1.9E-12 score: 47.5 coord: 151..290 e-value: 3.6E-7 score: 30.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 301..336
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 301..327
None	No IPR available	PANTHER	PTHR31218:SF215	WAT1-RELATED PROTEIN	coord: 1..305
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 210..294
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 9..119
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 1..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g_new0575.1	MC04g_new0575.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity