MC04g_new0530 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g_new0530
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein RALF-like 10
LocationMC04: 21357722 .. 21358243 (+)
RNA-Seq ExpressionMC04g_new0530
SyntenyMC04g_new0530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCAAGCCGCGACGACTATAGTCGCGTCCGAGAGCTTTAGTGGAGATAAATACAGAGAAAAGGAAGAAGATGAAGTCATGGATGATATGTCTGCTCTTAATTAGCATGTTGGCGATGAGCCAAGAAGTTGCTGCGGAGACAAAATATCTTAAAATTTTTGAGTGCCATCGGCCTAATCCACCTCCAAGTTGTCCTCCATCTGATCATCATAAGCATCATCGAAGAGTTCCTGCGAATCCTTATAATCGTGGCTGCTCTGCTATCCACCGATGTCGTGGAGGATGATGCAAATCAACATTTCCATTTCTATTTATCTACCACATATGAAGTGAAAAAAAGGACCAACTTCATATCTTATGCTGGACTGTTGAATAAATTGATATATGTATTGACTAATTCAACAGTTGAATGGATTTTTATTTAAATAAGTTACCAATCTTTTAGTTAGAGAAAATATAAAAACTTATATCCTCCTAATCAGATAAATCTAAGAGGTACATAAATGTGCATTTTGAAATAAAG

mRNA sequence

ATGAAGTCATGGATGATATGTCTGCTCTTAATTAGCATGTTGGCGATGAGCCAAGAAGTTGCTGCGGAGACAAAATATCTTAAAATTTTTGAGTGCCATCGGCCTAATCCACCTCCAAGTTGTCCTCCATCTGATCATCATAAGCATCATCGAAGAGTTCCTGCGAATCCTTATAATCGTGGCTGCTCTGCTATCCACCGATGTCGTGGAGGATGA

Coding sequence (CDS)

ATGAAGTCATGGATGATATGTCTGCTCTTAATTAGCATGTTGGCGATGAGCCAAGAAGTTGCTGCGGAGACAAAATATCTTAAAATTTTTGAGTGCCATCGGCCTAATCCACCTCCAAGTTGTCCTCCATCTGATCATCATAAGCATCATCGAAGAGTTCCTGCGAATCCTTATAATCGTGGCTGCTCTGCTATCCACCGATGTCGTGGAGGATGA

Protein sequence

MKSWMICLLLISMLAMSQEVAAETKYLKIFECHRPNPPPSCPPSDHHKHHRRVPANPYNRGCSAIHRCRGG
Homology
BLAST of MC04g_new0530 vs. ExPASy Swiss-Prot
Match: O64466 (Protein RALF-like 11 OS=Arabidopsis thaliana OX=3702 GN=RALFL11 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.7e-08
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAET--KYLKIFECHRPNPPPSC-PPSDHHKHHRRVPANP 60
          MK+W+ICLL+I    +++ V +    +Y  I +C  PNPPP C PP    K+    P N 
Sbjct: 1  MKAWLICLLVICAAVIAEPVESRNYIEYGAINKCAGPNPPPGCNPPGAEQKN--PTPVNE 60

Query: 61 YNRGCSAIHRCR 70
          Y+RGCS IHRCR
Sbjct: 61 YSRGCSKIHRCR 70

BLAST of MC04g_new0530 vs. ExPASy Swiss-Prot
Match: F4ISE2 (Protein RALF-like 13 OS=Arabidopsis thaliana OX=3702 GN=RALFL13 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 7.7e-08
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAET--KYLKIFECHRPNPPPSC-PPSDHHKHHRRVPANP 60
          MK+W+ICLL+I    +++ V +    +Y  I +C  PNPPP C PP    K+    P N 
Sbjct: 1  MKAWVICLLVICAAVIAEPVESRNYIEYGAINKCAGPNPPPGCNPPGAEQKNPN--PVNE 60

Query: 61 YNRGCSAIHRCR 70
          Y+RGCS IHRCR
Sbjct: 61 YSRGCSKIHRCR 70

BLAST of MC04g_new0530 vs. ExPASy Swiss-Prot
Match: F4ISE1 (Protein RALF-like 12 OS=Arabidopsis thaliana OX=3702 GN=RALFL12 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.8e-07
Identity = 32/72 (44.44%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAET--KYLKIFECHRPNPPPSC-PPSDHHKHHRRVPANP 60
          MK+W+I LL+I  + +++ V +    +Y  I +C  PNPPP C PP    K+    P N 
Sbjct: 1  MKAWVIGLLVICAVVIAEPVESRNYIEYGAINKCAGPNPPPGCNPPGTEQKN--PTPVNE 60

Query: 61 YNRGCSAIHRCR 70
          Y+RGCS IHRCR
Sbjct: 61 YSRGCSKIHRCR 70

BLAST of MC04g_new0530 vs. ExPASy Swiss-Prot
Match: O65919 (Protein RALF-like 10 OS=Arabidopsis thaliana OX=3702 GN=RALFL10 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.2e-05
Identity = 32/73 (43.84%), Postives = 42/73 (57.53%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAETKYLK---IFECHRPNPPPSC-PPSDHHKHHRRVPAN 60
          MK+ +ICLL+I    ++  V +  K+L    I +C  PNPPP C PP    K+    PAN
Sbjct: 1  MKALVICLLVIFAAVIAVPVESRRKHLDYGVITKCAGPNPPPGCYPPGAQQKN--PTPAN 60

Query: 61 PYNRGCSAIHRCR 70
           Y RGCS I RC+
Sbjct: 61 EYRRGCSKITRCK 71

BLAST of MC04g_new0530 vs. ExPASy Swiss-Prot
Match: A7REH2 (Protein RALF-like 30 OS=Arabidopsis thaliana OX=3702 GN=RALFL30 PE=3 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 5.2e-04
Identity = 31/74 (41.89%), Postives = 40/74 (54.05%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQE--VAAETKYLK---IFECHRPNPPPSC-PPSDHHKHHRRVP 60
          MK+W+ICL++IS+  M +    A   K+L    +  C RPNPPP C  P    K   RV 
Sbjct: 1  MKAWVICLMVISIFMMIEPTLAAGGGKFLNPGVLDPCLRPNPPPECQAPGSAGKPRERV- 60

Query: 61 ANPYNRGCSAIHRC 69
           N Y  GCS + RC
Sbjct: 61 -NEYKVGCSKLTRC 72

BLAST of MC04g_new0530 vs. NCBI nr
Match: XP_038877580.1 (protein RALF-like 11 [Benincasa hispida])

HSP 1 Score: 81.6 bits (200), Expect = 1.60e-18
Identity = 42/72 (58.33%), Postives = 52/72 (72.22%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAETKYLKIFE-CHRPNPPPSCPPSDHHKHHRRVPANPYN 60
          MKSW+IC++ ISML +   VAAE KYL+  + C++PNPPP+C          +V ANPYN
Sbjct: 1  MKSWLICVVFISMLVVGPGVAAEKKYLQFLDPCNQPNPPPTCSEVK-----EQVEANPYN 60

Query: 61 RGCSAIHRCRGG 71
          RGCSAIHRCRGG
Sbjct: 61 RGCSAIHRCRGG 67

BLAST of MC04g_new0530 vs. NCBI nr
Match: XP_022947821.1 (protein RALF-like 10 [Cucurbita moschata])

HSP 1 Score: 80.5 bits (197), Expect = 4.47e-18
Identity = 42/72 (58.33%), Postives = 52/72 (72.22%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAETKYLKIFE-CHRPNPPPSCPPSDHHKHHRRVPANPYN 60
          M+SW+ICL+LI ML +SQ+VA+ETK+LK  + C +PNPPP C   +       VPANPY 
Sbjct: 1  MRSWLICLVLIGMLVVSQDVASETKFLKFPDPCQQPNPPPGCSIGN------AVPANPYR 60

Query: 61 RGCSAIHRCRGG 71
          RGCS I RCRGG
Sbjct: 61 RGCSKITRCRGG 66

BLAST of MC04g_new0530 vs. NCBI nr
Match: KAE8637268.1 (hypothetical protein CSA_017684 [Cucumis sativus])

HSP 1 Score: 79.3 bits (194), Expect = 1.35e-17
Identity = 44/71 (61.97%), Postives = 51/71 (71.83%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAETKYLKIFE-CHRPNPPPSCPPSDHHKHHRRVPANPYN 60
          MKSW+IC++ ISML MSQEV A+T +LK    C  P+PPPSC  S   K H   P NPY+
Sbjct: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSC--SSKVKDH--APTNPYD 60

Query: 61 RGCSAIHRCRG 70
          RGCSAIHRCRG
Sbjct: 61 RGCSAIHRCRG 67

BLAST of MC04g_new0530 vs. NCBI nr
Match: XP_023533089.1 (protein RALF-like 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 78.6 bits (192), Expect = 2.58e-17
Identity = 42/72 (58.33%), Postives = 52/72 (72.22%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAETKYLKIFE-CHRPNPPPSCPPSDHHKHHRRVPANPYN 60
          M+SW+IC++LISML +SQEVA+ETK+LK  + C +PNPP  C   +       VPANPY 
Sbjct: 1  MRSWLICIVLISMLVVSQEVASETKFLKFPDPCQQPNPPLGCSIGN------AVPANPYR 60

Query: 61 RGCSAIHRCRGG 71
          RGCS I RCRGG
Sbjct: 61 RGCSKITRCRGG 66

BLAST of MC04g_new0530 vs. NCBI nr
Match: KAG7020863.1 (hypothetical protein SDJN02_17551, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 78.2 bits (191), Expect = 5.43e-17
Identity = 45/77 (58.44%), Postives = 52/77 (67.53%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEV------AAETKYLKIFECHRPNPPPSCPPSDHHKHHRRVP 60
          MKS  ICL LI+ML  SQ+V       ++ KYLK  ECH PNPP  C  S   K ++ VP
Sbjct: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGV-KGYQHVP 60

Query: 61 ANPYNRGCSAIHRCRGG 71
          ANPY+RGCSAIHRCRGG
Sbjct: 61 ANPYDRGCSAIHRCRGG 76

BLAST of MC04g_new0530 vs. ExPASy TrEMBL
Match: A0A0A0LI94 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G191310 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 6.81e-20
Identity = 44/72 (61.11%), Postives = 54/72 (75.00%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAETKYLKIFE-CHRPNPPPSCPPSDHHKHHRRVPANPYN 60
          MKSW+ICL LI ML + QEVAAETK+LK  + C++P+PPP+CP  D     +   ANPYN
Sbjct: 1  MKSWLICLALIGMLVVGQEVAAETKHLKFIDPCNQPSPPPTCPNRD-----KVEEANPYN 60

Query: 61 RGCSAIHRCRGG 71
          R CSAI+RCRGG
Sbjct: 61 RDCSAINRCRGG 67

BLAST of MC04g_new0530 vs. ExPASy TrEMBL
Match: A0A6J1G7J2 (protein RALF-like 10 OS=Cucurbita moschata OX=3662 GN=LOC111451581 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.16e-18
Identity = 42/72 (58.33%), Postives = 52/72 (72.22%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAETKYLKIFE-CHRPNPPPSCPPSDHHKHHRRVPANPYN 60
          M+SW+ICL+LI ML +SQ+VA+ETK+LK  + C +PNPPP C   +       VPANPY 
Sbjct: 1  MRSWLICLVLIGMLVVSQDVASETKFLKFPDPCQQPNPPPGCSIGN------AVPANPYR 60

Query: 61 RGCSAIHRCRGG 71
          RGCS I RCRGG
Sbjct: 61 RGCSKITRCRGG 66

BLAST of MC04g_new0530 vs. ExPASy TrEMBL
Match: A0A0A0M0X3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690160 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 6.53e-18
Identity = 44/71 (61.97%), Postives = 51/71 (71.83%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAETKYLKIFE-CHRPNPPPSCPPSDHHKHHRRVPANPYN 60
          MKSW+IC++ ISML MSQEV A+T +LK    C  P+PPPSC  S   K H   P NPY+
Sbjct: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSC--SSKVKDH--APTNPYD 60

Query: 61 RGCSAIHRCRG 70
          RGCSAIHRCRG
Sbjct: 61 RGCSAIHRCRG 67

BLAST of MC04g_new0530 vs. ExPASy TrEMBL
Match: A0A1S4DY33 (protein RALF-like 30 OS=Cucumis melo OX=3656 GN=LOC107991083 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.82e-14
Identity = 39/72 (54.17%), Postives = 48/72 (66.67%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAETKYLKIFE-CHRPNPPPSCPPSDHHKHHRRVPANPYN 60
          MKSW+IC  L+SML M  EVAA+TK+LK  + C +P+PP SC     +KH R      Y+
Sbjct: 1  MKSWLICFALLSMLVMGHEVAAKTKFLKFLDPCLQPSPPRSC-----YKHERVDSPIAYD 60

Query: 61 RGCSAIHRCRGG 71
          RGCS I RCRGG
Sbjct: 61 RGCSPILRCRGG 67

BLAST of MC04g_new0530 vs. ExPASy TrEMBL
Match: A0A0A0LZK3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G610770 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 5.46e-13
Identity = 39/72 (54.17%), Postives = 47/72 (65.28%), Query Frame = 0

Query: 1   MKSWMICLLLISMLAMSQEVAAETKYLKIFE-CHRPNPPPSCPPSDHHKHHRRVPANPYN 60
           MKSW+IC  L+SML MS EVAAE K+L   + C +P+PP SC     +K  R     PY+
Sbjct: 34  MKSWLICFALLSMLVMSHEVAAEGKFLNFSDLCLQPSPPKSC-----YKDERADSPIPYD 93

Query: 61  RGCSAIHRCRGG 71
           RGCS I RCRGG
Sbjct: 94  RGCSPILRCRGG 100

BLAST of MC04g_new0530 vs. TAIR 10
Match: AT2G19030.1 (ralf-like 11 )

HSP 1 Score: 58.9 bits (141), Expect = 1.9e-09
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAET--KYLKIFECHRPNPPPSC-PPSDHHKHHRRVPANP 60
          MK+W+ICLL+I    +++ V +    +Y  I +C  PNPPP C PP    K+    P N 
Sbjct: 1  MKAWLICLLVICAAVIAEPVESRNYIEYGAINKCAGPNPPPGCNPPGAEQKN--PTPVNE 60

Query: 61 YNRGCSAIHRCR 70
          Y+RGCS IHRCR
Sbjct: 61 YSRGCSKIHRCR 70

BLAST of MC04g_new0530 vs. TAIR 10
Match: AT2G19045.1 (RALF-like 13 )

HSP 1 Score: 57.4 bits (137), Expect = 5.5e-09
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAET--KYLKIFECHRPNPPPSC-PPSDHHKHHRRVPANP 60
          MK+W+ICLL+I    +++ V +    +Y  I +C  PNPPP C PP    K+    P N 
Sbjct: 1  MKAWVICLLVICAAVIAEPVESRNYIEYGAINKCAGPNPPPGCNPPGAEQKNPN--PVNE 60

Query: 61 YNRGCSAIHRCR 70
          Y+RGCS IHRCR
Sbjct: 61 YSRGCSKIHRCR 70

BLAST of MC04g_new0530 vs. TAIR 10
Match: AT2G19040.1 (RALF-like 12 )

HSP 1 Score: 55.1 bits (131), Expect = 2.7e-08
Identity = 32/72 (44.44%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAET--KYLKIFECHRPNPPPSC-PPSDHHKHHRRVPANP 60
          MK+W+I LL+I  + +++ V +    +Y  I +C  PNPPP C PP    K+    P N 
Sbjct: 1  MKAWVIGLLVICAVVIAEPVESRNYIEYGAINKCAGPNPPPGCNPPGTEQKN--PTPVNE 60

Query: 61 YNRGCSAIHRCR 70
          Y+RGCS IHRCR
Sbjct: 61 YSRGCSKIHRCR 70

BLAST of MC04g_new0530 vs. TAIR 10
Match: AT2G19020.1 (ralf-like 10 )

HSP 1 Score: 50.1 bits (118), Expect = 8.8e-07
Identity = 32/73 (43.84%), Postives = 42/73 (57.53%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQEVAAETKYLK---IFECHRPNPPPSC-PPSDHHKHHRRVPAN 60
          MK+ +ICLL+I    ++  V +  K+L    I +C  PNPPP C PP    K+    PAN
Sbjct: 1  MKALVICLLVIFAAVIAVPVESRRKHLDYGVITKCAGPNPPPGCYPPGAQQKN--PTPAN 60

Query: 61 PYNRGCSAIHRCR 70
           Y RGCS I RC+
Sbjct: 61 EYRRGCSKITRCK 71

BLAST of MC04g_new0530 vs. TAIR 10
Match: AT4G13075.1 (RALF-like 30 )

HSP 1 Score: 44.7 bits (104), Expect = 3.7e-05
Identity = 31/74 (41.89%), Postives = 40/74 (54.05%), Query Frame = 0

Query: 1  MKSWMICLLLISMLAMSQE--VAAETKYLK---IFECHRPNPPPSC-PPSDHHKHHRRVP 60
          MK+W+ICL++IS+  M +    A   K+L    +  C RPNPPP C  P    K   RV 
Sbjct: 1  MKAWVICLMVISIFMMIEPTLAAGGGKFLNPGVLDPCLRPNPPPECQAPGSAGKPRERV- 60

Query: 61 ANPYNRGCSAIHRC 69
           N Y  GCS + RC
Sbjct: 61 -NEYKVGCSKLTRC 72

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O644662.7e-0845.83Protein RALF-like 11 OS=Arabidopsis thaliana OX=3702 GN=RALFL11 PE=3 SV=1[more]
F4ISE27.7e-0845.83Protein RALF-like 13 OS=Arabidopsis thaliana OX=3702 GN=RALFL13 PE=3 SV=1[more]
F4ISE13.8e-0744.44Protein RALF-like 12 OS=Arabidopsis thaliana OX=3702 GN=RALFL12 PE=2 SV=1[more]
O659191.2e-0543.84Protein RALF-like 10 OS=Arabidopsis thaliana OX=3702 GN=RALFL10 PE=2 SV=1[more]
A7REH25.2e-0441.89Protein RALF-like 30 OS=Arabidopsis thaliana OX=3702 GN=RALFL30 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038877580.11.60e-1858.33protein RALF-like 11 [Benincasa hispida][more]
XP_022947821.14.47e-1858.33protein RALF-like 10 [Cucurbita moschata][more]
KAE8637268.11.35e-1761.97hypothetical protein CSA_017684 [Cucumis sativus][more]
XP_023533089.12.58e-1758.33protein RALF-like 10 [Cucurbita pepo subsp. pepo][more]
KAG7020863.15.43e-1758.44hypothetical protein SDJN02_17551, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
A0A0A0LI946.81e-2061.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G191310 PE=3 SV=1[more]
A0A6J1G7J22.16e-1858.33protein RALF-like 10 OS=Cucurbita moschata OX=3662 GN=LOC111451581 PE=3 SV=1[more]
A0A0A0M0X36.53e-1861.97Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690160 PE=3 SV=1[more]
A0A1S4DY335.82e-1454.17protein RALF-like 30 OS=Cucumis melo OX=3656 GN=LOC107991083 PE=3 SV=1[more]
A0A0A0LZK35.46e-1354.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G610770 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G19030.11.9e-0945.83ralf-like 11 [more]
AT2G19045.15.5e-0945.83RALF-like 13 [more]
AT2G19040.12.7e-0844.44RALF-like 12 [more]
AT2G19020.18.8e-0743.84ralf-like 10 [more]
AT4G13075.13.7e-0541.89RALF-like 30 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008801Rapid ALkalinization FactorPFAMPF05498RALFcoord: 23..70
e-value: 1.2E-8
score: 35.8
NoneNo IPR availablePANTHERPTHR34270:SF5PROTEIN RALF-LIKE 10-RELATEDcoord: 1..71
NoneNo IPR availablePANTHERPTHR34270PROTEIN RALF-LIKE 15-RELATEDcoord: 1..71

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g_new0530.1MC04g_new0530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0110165 cellular anatomical entity