MC04g_new0475 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g_new0475 vs. ExPASy Swiss-Prot
Match: Q9M2H9 (MATH domain and coiled-coil domain-containing protein At3g58370 OS=Arabidopsis thaliana OX=3702 GN=At3g58370 PE=4 SV=3)

HSP 1 Score: 59.7 bits (143), Expect = 6.4e-08
Identity = 39/133 (29.32%), Postives = 60/133 (45.11%), Query Frame = 0

Query: 178 EPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSF 237
           E  +N++TW   NFS L  +   SDPF + G +W +  +PKG     S  LSL L+ D  
Sbjct: 4   EEVDNKFTWVIKNFSTLQYDKIYSDPFVIGGCKWHLLAYPKGNKFNNS--LSLYLVVDDA 63

Query: 238 KELPQG-------------------------KYWFEFGDLGLGFRNFMSLRDLNEVTKGY 286
           + LP G                         + WF   +LG GF + + L +L+    GY
Sbjct: 64  RALPCGWRRYAQFSLTIINQLTDKLSQRGEKQNWFNQRNLGSGFTSMIPLPNLHAKNAGY 123

BLAST of MC04g_new0475 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 6.4e-08
Identity = 39/141 (27.66%), Postives = 62/141 (43.97%), Query Frame = 0

Query: 177 KEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDS 236
           ++P   ++TW   NFS+ +   + SD F V GY+W+I + PKG        LS+ L    
Sbjct: 50  EDPPTLKFTWTIPNFSRQNTRKHYSDVFVVGGYKWRILIFPKG---NNVDHLSMYLDVSD 109

Query: 237 FKELPQGKYWFEFGDLGL---------------------------GFRNFMSLRDLNEVT 291
              LP G  W  +    L                           GF +FM L +L + +
Sbjct: 110 AASLPYG--WSRYAQFSLAVVNQIHTRYTVRKETQHQFNARESDWGFTSFMPLSELYDPS 169

BLAST of MC04g_new0475 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 6.4e-08
Identity = 39/141 (27.66%), Postives = 63/141 (44.68%), Query Frame = 0

Query: 177 KEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDS 236
           ++P + ++TW    F++L+   + SD F V GY+W+I + PKG        LS+ L    
Sbjct: 49  EDPPSLKFTWTIPMFTRLNTRKHYSDVFVVGGYKWRILIFPKG---NNVDHLSMYLDVAD 108

Query: 237 FKELPQGKYWFEFGDLGL---------------------------GFRNFMSLRDLNEVT 291
              LP G  W  +    L                           GF +FM L +L E T
Sbjct: 109 AANLPYG--WSRYSQFSLAVVNQVNNRYSIRKETQHQFNARESDWGFTSFMPLSELYEPT 168

BLAST of MC04g_new0475 vs. ExPASy Swiss-Prot
Match: Q9M2I9 (MATH domain and coiled-coil domain-containing protein At3g58270 OS=Arabidopsis thaliana OX=3702 GN=At3g58270 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 7.1e-07
Identity = 45/147 (30.61%), Postives = 71/147 (48.30%), Query Frame = 0

Query: 53  SQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYL-VKAEDGIPTS-EVNVVFTFLVYDNLN 112
           S  F   G KW+L  +P G    NG + +SLYL V   + +P     +  F F V + L+
Sbjct: 26  SDEFFVDGCKWRLLAFPKG----NGVEKLSLYLAVAGSEFLPDGWRRHAYFHFSVVNQLS 85

Query: 113 DKYLAVQDEKVRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKG 172
           D+    ++ K   F +  ++W F  ++SL+  +D   GFLV       VD+ V +  GK 
Sbjct: 86  DELSQARETK-NWFDASTSDWGFTSMLSLKKLHDKDGGFLVNGELKIVVDVSVLEVIGKL 145

Query: 173 EVVSFIKEPKNNEYTWKTNNFSQLDKN 198
           +V      P  +E T  T   S+L++N
Sbjct: 146 DV------PVESEET-TTKALSELEEN 160

BLAST of MC04g_new0475 vs. ExPASy Swiss-Prot
Match: A8MQL1 (TNF receptor-associated factor homolog 1b OS=Arabidopsis thaliana OX=3702 GN=TRAF1B PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.9e-04
Identity = 22/63 (34.92%), Postives = 35/63 (55.56%), Query Frame = 0

Query: 183 EYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFKELPQ 242
           +YTWK   FS++ K  + S+ F   GY+W I ++P+GC       LSL L   ++ +L  
Sbjct: 69  QYTWKIPKFSEITKREHRSNVFEAGGYKWYILIYPQGCDVC--NHLSLFLCVANYDKLLP 128

Query: 243 GKY 246
           G +
Sbjct: 129 GSF 129

BLAST of MC04g_new0475 vs. NCBI nr
Match: XP_022146381.1 (ubiquitin carboxyl-terminal hydrolase 13-like [Momordica charantia])

HSP 1 Score: 521 bits (1341), Expect = 2.03e-185
Identity = 267/306 (87.25%), Postives = 267/306 (87.25%), Query Frame = 0

Query: 1   MQRNNTISPSSEAADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGG 60
           MQRNNTISPSSEAADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGG
Sbjct: 1   MQRNNTISPSSEAADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGG 60

Query: 61  YKWKLAVYPNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKYLAVQDEK 120
           YKWKLAVYPNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKYLAVQDEK
Sbjct: 61  YKWKLAVYPNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKYLAVQDEK 120

Query: 121 VRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPK 180
           VRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPK
Sbjct: 121 VRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPK 180

Query: 181 NNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFKEL 240
           NNEYTWKTNNFSQLDKNMYESDPFTVEGYR                          FKEL
Sbjct: 181 NNEYTWKTNNFSQLDKNMYESDPFTVEGYR-------------------------CFKEL 240

Query: 241 PQG-------------KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVV 293
           PQG             KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVV
Sbjct: 241 PQGSQVYTEFEIAVLSKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVV 281

BLAST of MC04g_new0475 vs. NCBI nr
Match: XP_022146382.1 (uncharacterized protein LOC111015610 [Momordica charantia])

HSP 1 Score: 414 bits (1063), Expect = 2.99e-142
Identity = 216/305 (70.82%), Postives = 240/305 (78.69%), Query Frame = 0

Query: 1   MQRNNTISPSSEA-ADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAG 60
           MQRN+ ISPSS+A + SVL   RDEKPLHYTLQIQ FSLLKTALA S+ DRYES IF+  
Sbjct: 36  MQRNHIISPSSQADSGSVLRSIRDEKPLHYTLQIQPFSLLKTALATSSRDRYESPIFSVA 95

Query: 61  GYKWKLAVYPNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKYLAVQDE 120
           G+KWKLAVYP+GDVKRNGS+HISLYLV AED IPT ++ VVFT LVYDNL D YLAV D 
Sbjct: 96  GFKWKLAVYPSGDVKRNGSNHISLYLVTAEDDIPTLDLTVVFTILVYDNLRDNYLAVLDG 155

Query: 121 KVRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEP 180
           KVRRFH+++ EW FEKL+SLETFNDSSNGFLV DCCAFGVDI V KCDGKGE +S IK+P
Sbjct: 156 KVRRFHAMKKEWGFEKLVSLETFNDSSNGFLVDDCCAFGVDIFVMKCDGKGEALSLIKQP 215

Query: 181 KNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFKE 240
           KNN+YTWKTNNFSQLD+NM             K+ L+PKG  +A   +LSL L  +S KE
Sbjct: 216 KNNKYTWKTNNFSQLDENM-------------KMSLYPKGSPKASPDYLSLFLRCESLKE 275

Query: 241 LPQG-------------KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINV 291
           LPQG             KYWFEFGDLGLGF +FMSLRDLNEVTKGYICDDTLIVEVKINV
Sbjct: 276 LPQGCRVYAEFEIAVLSKYWFEFGDLGLGFPDFMSLRDLNEVTKGYICDDTLIVEVKINV 327

BLAST of MC04g_new0475 vs. NCBI nr
Match: XP_022146380.1 (probable inactive serine/threonine-protein kinase fnkC [Momordica charantia])

HSP 1 Score: 382 bits (981), Expect = 1.69e-130
Identity = 197/291 (67.70%), Postives = 225/291 (77.32%), Query Frame = 0

Query: 17  VLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRN 76
           V+  TRDEKPLHYTLQIQSFSLLK ALA+SN DRYESQIFNAGGYKWKLAVYPNGDVKRN
Sbjct: 8   VIRSTRDEKPLHYTLQIQSFSLLKAALASSNRDRYESQIFNAGGYKWKLAVYPNGDVKRN 67

Query: 77  GSDHISLYLVKAEDGI-PTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIQTEWEFEK 136
           G+DHISLYLVKA+D I P S                        KVRRFH+++ EW FEK
Sbjct: 68  GADHISLYLVKADDDINPCSH----------------------GKVRRFHAMKAEWGFEK 127

Query: 137 LISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLD 196
           LISL+TFNDSSNGF+VGDCC FGVD+VV KCDGKGEVVSFIKEPK+++YTWKT NFSQLD
Sbjct: 128 LISLDTFNDSSNGFVVGDCCGFGVDVVVMKCDGKGEVVSFIKEPKSHKYTWKTKNFSQLD 187

Query: 197 KNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFKELPQGKY---------- 256
           K  YESDPFTVEGYRWK+ L+PKG S+A++GFLSL L ++S++E+PQG            
Sbjct: 188 KKFYESDPFTVEGYRWKLWLYPKGSSKAKTGFLSLYLSYNSYEEIPQGSQVYVEYEDSHN 247

Query: 257 -WFEFGDL----GLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLK 291
            WF FG +    G G  NF+SLRDLNE T+G+I DDT+IVEVKINVVSTLK
Sbjct: 248 CWFGFGGVESGRGWGHPNFLSLRDLNEPTEGFIVDDTIIVEVKINVVSTLK 276

BLAST of MC04g_new0475 vs. NCBI nr
Match: XP_038874970.1 (uncharacterized protein LOC120067490 [Benincasa hispida])

HSP 1 Score: 358 bits (918), Expect = 3.79e-120
Identity = 183/312 (58.65%), Postives = 229/312 (73.40%), Query Frame = 0

Query: 10  SSEAADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNC-DRYESQIFNAGGYKWKLAVY 69
           ++E +  V+  +RDEKPLHYTL+IQSFSLLK A+AAS+  +RYESQIF AGGYKWKLA+Y
Sbjct: 19  NNENSSLVIRTSRDEKPLHYTLKIQSFSLLKAAVAASSIRNRYESQIFKAGGYKWKLALY 78

Query: 70  PNGDVKRNGSDHISLYLVKAEDGIPTS--EVNVVFTFLVYDNLNDKYLAVQDEKVRRFHS 129
           PNGD KRN ++HISLYLV AED I +S  E+ V+  FLVYDNL D+Y  +QD KVR FH+
Sbjct: 79  PNGDFKRNVANHISLYLVSAEDEISSSGLEIKVMIKFLVYDNLKDRYWTIQDGKVRCFHA 138

Query: 130 IQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKC-DGKGEVVSFIKEPKNNEYT 189
           I+ EW FEKL+SL+TFND+SNG LV DCCAFGVD++V K  DG  E++S IK+ KN +YT
Sbjct: 139 IKKEWGFEKLVSLDTFNDTSNGLLVDDCCAFGVDVIVMKSYDGNNEILSLIKQLKNYKYT 198

Query: 190 WKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFKELPQG-- 249
           WK NNFSQL KN+YESDPFTV+ Y WKI L+P+G S A +G++S+ L FDSF++LPQG  
Sbjct: 199 WKINNFSQLSKNVYESDPFTVDNYTWKIELYPRGDSLAMTGYMSMFLRFDSFEKLPQGSR 258

Query: 250 ----------------------KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVE 293
                                 K+WF+      GF NFMSL ++ E  KG++CDDTL+VE
Sbjct: 259 VYVEYDIAVLSQLGGAYTNYPVKHWFDGETKSWGFGNFMSLSNVKEPMKGFLCDDTLMVE 318

BLAST of MC04g_new0475 vs. NCBI nr
Match: XP_038875772.1 (uncharacterized protein LOC120068142 [Benincasa hispida])

HSP 1 Score: 355 bits (912), Expect = 2.44e-119
Identity = 190/321 (59.19%), Postives = 225/321 (70.09%), Query Frame = 0

Query: 4   NNTISPS--SEAADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGY 63
           NNT+SP+   + + SV+  TR EKP+HYTL+IQSFSLLK  LA+S   R+ESQ FNAG Y
Sbjct: 10  NNTVSPNPHGDNSSSVVMSTRHEKPVHYTLKIQSFSLLKNGLASSQRQRFESQKFNAGCY 69

Query: 64  KWKLAVYPNGDVKRNGSDHISLYLVKAEDGI--PTSEVNVVFTFLVYDNLNDKYLAVQDE 123
           +WKLA+YPNGD +RN S+HISLYLV   + I   TSEVNVVFTFLVYD L  KYL VQD 
Sbjct: 70  EWKLALYPNGDERRNVSNHISLYLVIVGNNILSTTSEVNVVFTFLVYDTLRGKYLTVQDG 129

Query: 124 KVRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDG-KGEVVSFIKE 183
           K+RRF +++TEW  EKL+ L+TFND+SNGFLV DCC FGVDI V  CDG  GEV S IK+
Sbjct: 130 KMRRFSAMKTEWGIEKLLPLDTFNDASNGFLVDDCCVFGVDIFVMSCDGGMGEVFSLIKQ 189

Query: 184 PKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFK 243
           PKN EYTWK NNFSQL   + ES+PFTVE YRWKI L+P GCS+A+SGFLS+ L+FDS  
Sbjct: 190 PKNYEYTWKLNNFSQLGSTLKESNPFTVESYRWKILLYPSGCSKAKSGFLSMYLLFDSSN 249

Query: 244 ELPQGKY------------------------WFEFGDL----GLGFRNFMSLRDLNEVTK 291
           +LPQG                          WF F  +    G G  NF SL DL E TK
Sbjct: 250 QLPQGSQVYVEYEMAILSQLEAVPVKETYNCWFGFDGVEAGRGWGAPNFSSLSDLEEPTK 309

BLAST of MC04g_new0475 vs. ExPASy TrEMBL
Match: A0A6J1CZ69 (ubiquitin carboxyl-terminal hydrolase 13-like OS=Momordica charantia OX=3673 GN=LOC111015609 PE=4 SV=1)

HSP 1 Score: 521 bits (1341), Expect = 9.84e-186
Identity = 267/306 (87.25%), Postives = 267/306 (87.25%), Query Frame = 0

Query: 1   MQRNNTISPSSEAADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGG 60
           MQRNNTISPSSEAADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGG
Sbjct: 1   MQRNNTISPSSEAADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGG 60

Query: 61  YKWKLAVYPNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKYLAVQDEK 120
           YKWKLAVYPNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKYLAVQDEK
Sbjct: 61  YKWKLAVYPNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKYLAVQDEK 120

Query: 121 VRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPK 180
           VRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPK
Sbjct: 121 VRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPK 180

Query: 181 NNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFKEL 240
           NNEYTWKTNNFSQLDKNMYESDPFTVEGYR                          FKEL
Sbjct: 181 NNEYTWKTNNFSQLDKNMYESDPFTVEGYR-------------------------CFKEL 240

Query: 241 PQG-------------KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVV 293
           PQG             KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVV
Sbjct: 241 PQGSQVYTEFEIAVLSKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVV 281

BLAST of MC04g_new0475 vs. ExPASy TrEMBL
Match: A0A6J1CZF7 (uncharacterized protein LOC111015610 OS=Momordica charantia OX=3673 GN=LOC111015610 PE=4 SV=1)

HSP 1 Score: 414 bits (1063), Expect = 1.45e-142
Identity = 216/305 (70.82%), Postives = 240/305 (78.69%), Query Frame = 0

Query: 1   MQRNNTISPSSEA-ADSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAG 60
           MQRN+ ISPSS+A + SVL   RDEKPLHYTLQIQ FSLLKTALA S+ DRYES IF+  
Sbjct: 36  MQRNHIISPSSQADSGSVLRSIRDEKPLHYTLQIQPFSLLKTALATSSRDRYESPIFSVA 95

Query: 61  GYKWKLAVYPNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKYLAVQDE 120
           G+KWKLAVYP+GDVKRNGS+HISLYLV AED IPT ++ VVFT LVYDNL D YLAV D 
Sbjct: 96  GFKWKLAVYPSGDVKRNGSNHISLYLVTAEDDIPTLDLTVVFTILVYDNLRDNYLAVLDG 155

Query: 121 KVRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEP 180
           KVRRFH+++ EW FEKL+SLETFNDSSNGFLV DCCAFGVDI V KCDGKGE +S IK+P
Sbjct: 156 KVRRFHAMKKEWGFEKLVSLETFNDSSNGFLVDDCCAFGVDIFVMKCDGKGEALSLIKQP 215

Query: 181 KNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFKE 240
           KNN+YTWKTNNFSQLD+NM             K+ L+PKG  +A   +LSL L  +S KE
Sbjct: 216 KNNKYTWKTNNFSQLDENM-------------KMSLYPKGSPKASPDYLSLFLRCESLKE 275

Query: 241 LPQG-------------KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINV 291
           LPQG             KYWFEFGDLGLGF +FMSLRDLNEVTKGYICDDTLIVEVKINV
Sbjct: 276 LPQGCRVYAEFEIAVLSKYWFEFGDLGLGFPDFMSLRDLNEVTKGYICDDTLIVEVKINV 327

BLAST of MC04g_new0475 vs. ExPASy TrEMBL
Match: A0A6J1CX47 (probable inactive serine/threonine-protein kinase fnkC OS=Momordica charantia OX=3673 GN=LOC111015608 PE=4 SV=1)

HSP 1 Score: 382 bits (981), Expect = 8.20e-131
Identity = 197/291 (67.70%), Postives = 225/291 (77.32%), Query Frame = 0

Query: 17  VLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRN 76
           V+  TRDEKPLHYTLQIQSFSLLK ALA+SN DRYESQIFNAGGYKWKLAVYPNGDVKRN
Sbjct: 8   VIRSTRDEKPLHYTLQIQSFSLLKAALASSNRDRYESQIFNAGGYKWKLAVYPNGDVKRN 67

Query: 77  GSDHISLYLVKAEDGI-PTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIQTEWEFEK 136
           G+DHISLYLVKA+D I P S                        KVRRFH+++ EW FEK
Sbjct: 68  GADHISLYLVKADDDINPCSH----------------------GKVRRFHAMKAEWGFEK 127

Query: 137 LISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLD 196
           LISL+TFNDSSNGF+VGDCC FGVD+VV KCDGKGEVVSFIKEPK+++YTWKT NFSQLD
Sbjct: 128 LISLDTFNDSSNGFVVGDCCGFGVDVVVMKCDGKGEVVSFIKEPKSHKYTWKTKNFSQLD 187

Query: 197 KNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFKELPQGKY---------- 256
           K  YESDPFTVEGYRWK+ L+PKG S+A++GFLSL L ++S++E+PQG            
Sbjct: 188 KKFYESDPFTVEGYRWKLWLYPKGSSKAKTGFLSLYLSYNSYEEIPQGSQVYVEYEDSHN 247

Query: 257 -WFEFGDL----GLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLK 291
            WF FG +    G G  NF+SLRDLNE T+G+I DDT+IVEVKINVVSTLK
Sbjct: 248 CWFGFGGVESGRGWGHPNFLSLRDLNEPTEGFIVDDTIIVEVKINVVSTLK 276

BLAST of MC04g_new0475 vs. ExPASy TrEMBL
Match: A0A6J1CXZ0 (ubiquitin carboxyl-terminal hydrolase 12-like OS=Momordica charantia OX=3673 GN=LOC111015607 PE=4 SV=1)

HSP 1 Score: 323 bits (827), Expect = 2.05e-107
Identity = 190/324 (58.64%), Postives = 212/324 (65.43%), Query Frame = 0

Query: 1   MQRNNTI--SPSSEA-ADS---VLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQ 60
           M+R N I  S SSEA  DS   V+  TRDEKPLHYTLQIQSFSLLKTAL  S+CDRYES+
Sbjct: 1   MERMNIIFHSLSSEADTDSGCVVIRSTRDEKPLHYTLQIQSFSLLKTALVRSDCDRYESR 60

Query: 61  IFNAGGYKWKLAVYPNGDVKRNGS-DHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDKY 120
           IFNAGGYKWKLA+YP GDVKRNGS DHISLYLVKAED IPTSEVNVVFTFLVYD+L DKY
Sbjct: 61  IFNAGGYKWKLAMYPTGDVKRNGSHDHISLYLVKAEDDIPTSEVNVVFTFLVYDSLKDKY 120

Query: 121 LAVQDEKVRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVV 180
           LAVQD  VRRFH+I+TEW FEKLISL+TFNDSSNGFLV DCCAFGVD+VV K        
Sbjct: 121 LAVQDGIVRRFHAIKTEWGFEKLISLDTFNDSSNGFLVDDCCAFGVDVVVMK-------- 180

Query: 181 SFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLM 240
                                           +EGYRWK+ L+PKGCS A +GFLSL L+
Sbjct: 181 --------------------------------LEGYRWKLWLYPKGCSNAETGFLSLYLI 240

Query: 241 FDSFKELPQGK------------------------YWFEFGDLGLGFRNFMSLRDLNEVT 293
            DS KE+ QG                         YWF  G       NF+SL +L +  
Sbjct: 241 LDSSKEILQGSKVYVEYELALLSQLGAEPEKETYNYWFAGGG-----PNFISLSELKKPL 279

BLAST of MC04g_new0475 vs. ExPASy TrEMBL
Match: A0A6J1CYF6 (uncharacterized protein LOC111015611 OS=Momordica charantia OX=3673 GN=LOC111015611 PE=4 SV=1)

HSP 1 Score: 324 bits (830), Expect = 5.90e-104
Identity = 183/306 (59.80%), Postives = 199/306 (65.03%), Query Frame = 0

Query: 15  DSVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVK 74
           + +L   RDEKPLHYTLQIQ FSLLKTALA  + +RYES IFN GG+KWKLA+YPNGD  
Sbjct: 312 NDILRSIRDEKPLHYTLQIQPFSLLKTALATCSRERYESPIFNVGGFKWKLALYPNGDKN 371

Query: 75  RNGSDHISLYLVKAEDGI--PTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIQTEWE 134
           RNGS +ISLYLV AED I   TSEVNVV TFLVYDNL D YLAV D KVRRFH+++TEW 
Sbjct: 372 RNGSGYISLYLVTAEDEIVSATSEVNVVLTFLVYDNLRDNYLAVLDGKVRRFHAMKTEWG 431

Query: 135 FEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFS 194
           F KL+SLETFNDSSNGFLV DCCAFGVDI V K                           
Sbjct: 432 FGKLVSLETFNDSSNGFLVDDCCAFGVDIFVMK--------------------------- 491

Query: 195 QLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFKEL-PQG-------- 254
                        +E YRWKIRLHPKGCSQ  +GFLSL L+FDSFKEL PQG        
Sbjct: 492 -------------LESYRWKIRLHPKGCSQESAGFLSLYLIFDSFKELLPQGCRVYAEFE 551

Query: 255 ----------------KYWFEFGDL--GLGFRNFMSLRDLNEVTKGYICDDTLIVEVKIN 291
                           K WFEFG +  GLGF  FM LRDL EV KGYICDDTLIV+VKIN
Sbjct: 552 IAVLSKYGVGHKKLESKCWFEFGGIETGLGFPEFMPLRDLKEVKKGYICDDTLIVKVKIN 577

BLAST of MC04g_new0475 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 187.2 bits (474), Expect = 1.9e-47
Identity = 102/246 (41.46%), Postives = 144/246 (58.54%), Query Frame = 0

Query: 10  SSEAADSVLSFTRDEKPLHYTLQIQSFSLL-KTALAASNCDRYESQIFNAGGYKWKLAVY 69
           SS   D ++    D  P HY ++I+SFSLL K A+     +RYE++ F AGGYKWKL +Y
Sbjct: 3   SSGDYDEIIKSISDAPPTHYMVKIESFSLLTKHAI-----ERYETESFEAGGYKWKLVLY 62

Query: 70  PNGDVKRNGSDHISLYLVKAEDG--IPTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHS 129
           PNG+  +N  DH+S+YL  A+     P  EV  VF   + D   D YL +Q  + RRFHS
Sbjct: 63  PNGNKSKNTKDHVSVYLSLADSSSLSPGWEVYAVFRLYLLDQNKDNYLILQGNE-RRFHS 122

Query: 130 IQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKK--CDGKGEVVSFIKEPKNNEY 189
           ++ EW F+K I   TF+D+SNG+L+ D C FG D+ V K    G+GE +S IK+  ++++
Sbjct: 123 VKREWGFDKFIPTGTFSDASNGYLMEDTCMFGADVFVSKERRSGRGECLSMIKDATSSKH 182

Query: 190 TWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARSGFLSLKLMFDSFKELPQG- 249
            WK  NFS+LDK  Y+S+ F     +WKI  +P G  Q     LS+ L     + +  G 
Sbjct: 183 VWKIENFSKLDKESYDSNAFFAGDRKWKIEFYPTGTKQGTGTHLSIYLTLVDPETISDGT 242

BLAST of MC04g_new0475 vs. TAIR 10
Match: AT2G04170.1 (TRAF-like family protein )

HSP 1 Score: 172.6 bits (436), Expect = 4.8e-43
Identity = 101/298 (33.89%), Postives = 155/298 (52.01%), Query Frame = 0

Query: 22  RDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDHI 81
           RDE+P +  L I SFS++K        + YES +F AGGYKW+L +Y NG+    G++HI
Sbjct: 125 RDERPSNKILTITSFSVIK-----GRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHI 184

Query: 82  SLYL-VKAEDGIPTS-EVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIQTEWEFEKLISL 141
           SLY+ ++  + +P   EVNV     VY+    KYL V+D  V+R++  + EW + KLI L
Sbjct: 185 SLYVRIEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVKRYNDAKKEWGYGKLIPL 244

Query: 142 ETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKNMY 201
            TF D++ G+L  D  +FG +I         E V+FI  P NN +TWK  +FS L+   Y
Sbjct: 245 TTFLDTNEGYLEQDIASFGAEIFSGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFY 304

Query: 202 ESDPFTVEGYRWKIRLHPKGCSQARS----------------------GFLSLKL---MF 261
            SD F VE   W++  +PKG    RS                      G ++L+L     
Sbjct: 305 YSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRS 364

Query: 262 DSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 293
            +  ++    ++    D G+G    +SL + N+ +KGY  +D++I E ++  VS   +
Sbjct: 365 SNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVTNI 417

BLAST of MC04g_new0475 vs. TAIR 10
Match: AT2G04170.2 (TRAF-like family protein )

HSP 1 Score: 172.6 bits (436), Expect = 4.8e-43
Identity = 101/298 (33.89%), Postives = 155/298 (52.01%), Query Frame = 0

Query: 22  RDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDHI 81
           RDE+P +  L I SFS++K        + YES +F AGGYKW+L +Y NG+    G++HI
Sbjct: 125 RDERPSNKILTITSFSVIK-----GRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHI 184

Query: 82  SLYL-VKAEDGIPTS-EVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIQTEWEFEKLISL 141
           SLY+ ++  + +P   EVNV     VY+    KYL V+D  V+R++  + EW + KLI L
Sbjct: 185 SLYVRIEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVKRYNDAKKEWGYGKLIPL 244

Query: 142 ETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKNMY 201
            TF D++ G+L  D  +FG +I         E V+FI  P NN +TWK  +FS L+   Y
Sbjct: 245 TTFLDTNEGYLEQDIASFGAEIFSGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFY 304

Query: 202 ESDPFTVEGYRWKIRLHPKGCSQARS----------------------GFLSLKL---MF 261
            SD F VE   W++  +PKG    RS                      G ++L+L     
Sbjct: 305 YSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRS 364

Query: 262 DSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 293
            +  ++    ++    D G+G    +SL + N+ +KGY  +D++I E ++  VS   +
Sbjct: 365 SNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVTNI 417

BLAST of MC04g_new0475 vs. TAIR 10
Match: AT2G04170.3 (TRAF-like family protein )

HSP 1 Score: 172.6 bits (436), Expect = 4.8e-43
Identity = 101/298 (33.89%), Postives = 155/298 (52.01%), Query Frame = 0

Query: 22  RDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDHI 81
           RDE+P +  L I SFS++K        + YES +F AGGYKW+L +Y NG+    G++HI
Sbjct: 3   RDERPSNKILTITSFSVIK-----GRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHI 62

Query: 82  SLYL-VKAEDGIPTS-EVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIQTEWEFEKLISL 141
           SLY+ ++  + +P   EVNV     VY+    KYL V+D  V+R++  + EW + KLI L
Sbjct: 63  SLYVRIEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVKRYNDAKKEWGYGKLIPL 122

Query: 142 ETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKNMY 201
            TF D++ G+L  D  +FG +I         E V+FI  P NN +TWK  +FS L+   Y
Sbjct: 123 TTFLDTNEGYLEQDIASFGAEIFSGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFY 182

Query: 202 ESDPFTVEGYRWKIRLHPKGCSQARS----------------------GFLSLKL---MF 261
            SD F VE   W++  +PKG    RS                      G ++L+L     
Sbjct: 183 YSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRS 242

Query: 262 DSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 293
            +  ++    ++    D G+G    +SL + N+ +KGY  +D++I E ++  VS   +
Sbjct: 243 SNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVTNI 295

BLAST of MC04g_new0475 vs. TAIR 10
Match: AT2G04170.4 (TRAF-like family protein )

HSP 1 Score: 172.6 bits (436), Expect = 4.8e-43
Identity = 101/298 (33.89%), Postives = 155/298 (52.01%), Query Frame = 0

Query: 22  RDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDHI 81
           RDE+P +  L I SFS++K        + YES +F AGGYKW+L +Y NG+    G++HI
Sbjct: 3   RDERPSNKILTITSFSVIK-----GRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHI 62

Query: 82  SLYL-VKAEDGIPTS-EVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIQTEWEFEKLISL 141
           SLY+ ++  + +P   EVNV     VY+    KYL V+D  V+R++  + EW + KLI L
Sbjct: 63  SLYVRIEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVKRYNDAKKEWGYGKLIPL 122

Query: 142 ETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKNMY 201
            TF D++ G+L  D  +FG +I         E V+FI  P NN +TWK  +FS L+   Y
Sbjct: 123 TTFLDTNEGYLEQDIASFGAEIFSGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFY 182

Query: 202 ESDPFTVEGYRWKIRLHPKGCSQARS----------------------GFLSLKL---MF 261
            SD F VE   W++  +PKG    RS                      G ++L+L     
Sbjct: 183 YSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRS 242

Query: 262 DSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 293
            +  ++    ++    D G+G    +SL + N+ +KGY  +D++I E ++  VS   +
Sbjct: 243 SNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVTNI 295