MC04g_new0453 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g_new0453
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionReceptor-like serine/threonine-protein kinase
LocationMC04: 18099345 .. 18101732 (-)
RNA-Seq ExpressionMC04g_new0453
SyntenyMC04g_new0453
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCTTCCCCTCCTCTGCCTTCTCTTCTTCTTCACATCTCCGGCGGCCGCCCAACCGCCGCCGGCACCCAAGCCCCGCAACTTCTCCGCCTTCTCCATCTCCCAATCCCCATGGCGGCCTGAACAAAACCTCATTCTCCTCTCGCCCAACTCCCTTTTCGCCGCCGGCTTCCGCCCATCAACCAACAACTCCAATCTCTACGTCTTCTCCGTTTGGTTCCTCAACGTCTCCACCGACAACGTCGTTTGGTCCGCCAATCGTCTTTCTCCGGTAAACCGCTCGGCGGCTCTGGCCATCACGTCCTCCGGCCAGCTCCGTCTCAACGACGGCTCCGGCCGAAACCTCTGGCCTTCTAACAATGTTTTTGGGAATCCAAATTCGACGAAACTGATGCTTCGCGACGATGGGGATCTTATCTACGCAACGTGGGAGAGCTTCCAATTCCCCACCAACACCATTTTGCCAAATCAGACGTTTAACGAAACCACCATCGTCTCCAACAACGGCAAATATGAGTTCAAGAACTCTGTTAATTTGACGTTTGGTACAGCTACGTATTGGAACTCCGGAAATCCCTTCAATATTTTTCAAACTGATGGTCGAATCATCAGAAACAACCAAGGCCCAATTTTCCCCTCTGATTTTAACTCGACCCGACTGCGGAAATTGGTTGTTGATGATGATGGGAACCTCAAGATTTTCAGCTTCAACTCCAATCTCCAGCGGTGGGGTGTGGTTTGGCAAGCGCACGTTGAGTTGTGTCAGATTTATGCAACTTGTGGCCCGAACTCCATTTGTATGAGTGGTGGCAGCTACAATTCCACCTACTGCGTCTGCCCTCCGGGATTCATCCCGGATTCTCGCGGCGGAGCACGGCAAGGATGCCACCGGAAACTCAAGGTATCAAAGAAAGTCAAGTTTCTTCCGCTCGATTTTGTGACTTTCAGAGGTGGGGTTAAAGAAATTTCCTTGCAACTCCCAAATATTTCGACGTGTGAAGCGAATTGCGTGAAGAATGCGAGCTGCGTTGGGTATACGTTCAGTTTCGATGGAAGCGCTGTGTGTGCGCTTCAGTTAGACATTTTGTCGAACGGGTTGTGGTCGCCCGGGATGAAGAAAGCTGCCTTTGTGAAGGTCGAAAATTCGGAAACTGATCGCTCAAACTTCACCGGAATGATGTACAGACTCGAATCGACGTGTCCGGTTCACATCACCCTCCGCCCGCCGCCGGAAGATAAAGACAACACCACCAGAAACGTACTGATAATCATCTCCATATTCGTGGCGGAACTAATTTCGGGGGCGGTTTTCTTCTGTGCATTCTTGAAGAGATTCGTAAAATACAGAGACATGGCTCGCACGCTCGGTCTGGAGTCGCTCCCCGCCGGCGGGCCGAAGCGGTTCACCTACGCCGAGCTGAAAACTGCCACCAACGACTTCTCGAACTCCATCGGAAGAGGCGGATTCGGCGAGGTCTTCAAAGGGGAGTTGCCGGATAAACGCGTGGTCGCCGTCAAGTGTTTGAAAAACGTTGCCGGCGGCGACGGCGACTTCTGGTCAGAGGTCACCATCATCGCCCGAATGCACCACCTCAACTTGCTCCGATTGTGGGGATTCTGCGCCGAAAAGGGTCAAAGAATGCTGGTCTACGAGTACATCCCCAATGGATCTCTCGACAAATTCCTGTTCATCAAACCGCAGCCCTCTGATTCGGAGACGGACGCAACAGAGACGGCCGGAGAAAACCCCTCACTCGATTGGAGCGTCCGGTACCGAATCGCCATAGGAGTGGCCAGAGCAATCGCGTATCTGCACGAGGAATGTCTGGAGTGGGTGTTACATCGAGACATAAAACCCGAAAACATCCTTCTGGACAACGATTTCTGCCCGAAACTATCGGATTTCGGGCTGTCGAAACTGAGGAAAAACGACGAGACGGCGGTGAGCATGTCTCGGATCAGAGGGACGCCGGGTTACGTGGCGCCGGAGCTGGTGAAATTGGGTTCGAATTCAATAACGACGAAGGCCGACGTGTACAGCTTCGGAATGGTGCTGCTGGAGATCATCAGCGGGACGAGGAACTTCGAGACGAAAAGATCGACGGTGGAGAGCGCGGATTGGTACTTTCCGGGATGGGCGTTCGAGAAAGCGTTCGTGGAGGAGAAGATCGAAGAGATTCTAGACCGTCGGATTAGGAATGAGTACGACAGCGGATCCCATTTTGCTATGGTAAATCGTATGGTTTCAACGGCGATGTGGTGCCTTCAGAACCAGCCGGAGATGCGGCCGCCGATGGGGAAAGTCGTGAAGATGTTGGAAGGAAAGCTCGAAATTCCTCCCCCGGATAAGCCCTCCATTTACTTCTTGTCAGAACCCCAAAATTAA

mRNA sequence

ATGTCTCTTCCCCTCCTCTGCCTTCTCTTCTTCTTCACATCTCCGGCGGCCGCCCAACCGCCGCCGGCACCCAAGCCCCGCAACTTCTCCGCCTTCTCCATCTCCCAATCCCCATGGCGGCCTGAACAAAACCTCATTCTCCTCTCGCCCAACTCCCTTTTCGCCGCCGGCTTCCGCCCATCAACCAACAACTCCAATCTCTACGTCTTCTCCGTTTGGTTCCTCAACGTCTCCACCGACAACGTCGTTTGGTCCGCCAATCGTCTTTCTCCGGTAAACCGCTCGGCGGCTCTGGCCATCACGTCCTCCGGCCAGCTCCGTCTCAACGACGGCTCCGGCCGAAACCTCTGGCCTTCTAACAATGTTTTTGGGAATCCAAATTCGACGAAACTGATGCTTCGCGACGATGGGGATCTTATCTACGCAACGTGGGAGAGCTTCCAATTCCCCACCAACACCATTTTGCCAAATCAGACGTTTAACGAAACCACCATCGTCTCCAACAACGGCAAATATGAGTTCAAGAACTCTGTTAATTTGACGTTTGGTACAGCTACGTATTGGAACTCCGGAAATCCCTTCAATATTTTTCAAACTGATGGTCGAATCATCAGAAACAACCAAGGCCCAATTTTCCCCTCTGATTTTAACTCGACCCGACTGCGGAAATTGGTTGTTGATGATGATGGGAACCTCAAGATTTTCAGCTTCAACTCCAATCTCCAGCGGTGGGGTGTGGTTTGGCAAGCGCACGTTGAGTTGTGTCAGATTTATGCAACTTGTGGCCCGAACTCCATTTGTATGAGTGGTGGCAGCTACAATTCCACCTACTGCGTCTGCCCTCCGGGATTCATCCCGGATTCTCGCGGCGGAGCACGGCAAGGATGCCACCGGAAACTCAAGGTATCAAAGAAAGTCAAGTTTCTTCCGCTCGATTTTGTGACTTTCAGAGGTGGGGTTAAAGAAATTTCCTTGCAACTCCCAAATATTTCGACGTGTGAAGCGAATTGCGTGAAGAATGCGAGCTGCGTTGGGTATACGTTCAGTTTCGATGGAAGCGCTGTGTGTGCGCTTCAGTTAGACATTTTGTCGAACGGGTTGTGGTCGCCCGGGATGAAGAAAGCTGCCTTTGTGAAGGTCGAAAATTCGGAAACTGATCGCTCAAACTTCACCGGAATGATGTACAGACTCGAATCGACGTGTCCGGTTCACATCACCCTCCGCCCGCCGCCGGAAGATAAAGACAACACCACCAGAAACGTACTGATAATCATCTCCATATTCGTGGCGGAACTAATTTCGGGGGCGGTTTTCTTCTGTGCATTCTTGAAGAGATTCGTAAAATACAGAGACATGGCTCGCACGCTCGGTCTGGAGTCGCTCCCCGCCGGCGGGCCGAAGCGGTTCACCTACGCCGAGCTGAAAACTGCCACCAACGACTTCTCGAACTCCATCGGAAGAGGCGGATTCGGCGAGGTCTTCAAAGGGGAGTTGCCGGATAAACGCGTGGTCGCCGTCAAGTGTTTGAAAAACGTTGCCGGCGGCGACGGCGACTTCTGGTCAGAGGTCACCATCATCGCCCGAATGCACCACCTCAACTTGCTCCGATTGTGGGGATTCTGCGCCGAAAAGGGTCAAAGAATGCTGGTCTACGAGTACATCCCCAATGGATCTCTCGACAAATTCCTGTTCATCAAACCGCAGCCCTCTGATTCGGAGACGGACGCAACAGAGACGGCCGGAGAAAACCCCTCACTCGATTGGAGCGTCCGGTACCGAATCGCCATAGGAGTGGCCAGAGCAATCGCGTATCTGCACGAGGAATGTCTGGAGTGGGTGTTACATCGAGACATAAAACCCGAAAACATCCTTCTGGACAACGATTTCTGCCCGAAACTATCGGATTTCGGGCTGTCGAAACTGAGGAAAAACGACGAGACGGCGGTGAGCATGTCTCGGATCAGAGGGACGCCGGGTTACGTGGCGCCGGAGCTGGTGAAATTGGGTTCGAATTCAATAACGACGAAGGCCGACGTGTACAGCTTCGGAATGGTGCTGCTGGAGATCATCAGCGGGACGAGGAACTTCGAGACGAAAAGATCGACGGTGGAGAGCGCGGATTGGTACTTTCCGGGATGGGCGTTCGAGAAAGCGTTCGTGGAGGAGAAGATCGAAGAGATTCTAGACCGTCGGATTAGGAATGAGTACGACAGCGGATCCCATTTTGCTATGGTAAATCGTATGGTTTCAACGGCGATGTGGTGCCTTCAGAACCAGCCGGAGATGCGGCCGCCGATGGGGAAAGTCGTGAAGATGTTGGAAGGAAAGCTCGAAATTCCTCCCCCGGATAAGCCCTCCATTTACTTCTTGTCAGAACCCCAAAATTAA

Coding sequence (CDS)

ATGTCTCTTCCCCTCCTCTGCCTTCTCTTCTTCTTCACATCTCCGGCGGCCGCCCAACCGCCGCCGGCACCCAAGCCCCGCAACTTCTCCGCCTTCTCCATCTCCCAATCCCCATGGCGGCCTGAACAAAACCTCATTCTCCTCTCGCCCAACTCCCTTTTCGCCGCCGGCTTCCGCCCATCAACCAACAACTCCAATCTCTACGTCTTCTCCGTTTGGTTCCTCAACGTCTCCACCGACAACGTCGTTTGGTCCGCCAATCGTCTTTCTCCGGTAAACCGCTCGGCGGCTCTGGCCATCACGTCCTCCGGCCAGCTCCGTCTCAACGACGGCTCCGGCCGAAACCTCTGGCCTTCTAACAATGTTTTTGGGAATCCAAATTCGACGAAACTGATGCTTCGCGACGATGGGGATCTTATCTACGCAACGTGGGAGAGCTTCCAATTCCCCACCAACACCATTTTGCCAAATCAGACGTTTAACGAAACCACCATCGTCTCCAACAACGGCAAATATGAGTTCAAGAACTCTGTTAATTTGACGTTTGGTACAGCTACGTATTGGAACTCCGGAAATCCCTTCAATATTTTTCAAACTGATGGTCGAATCATCAGAAACAACCAAGGCCCAATTTTCCCCTCTGATTTTAACTCGACCCGACTGCGGAAATTGGTTGTTGATGATGATGGGAACCTCAAGATTTTCAGCTTCAACTCCAATCTCCAGCGGTGGGGTGTGGTTTGGCAAGCGCACGTTGAGTTGTGTCAGATTTATGCAACTTGTGGCCCGAACTCCATTTGTATGAGTGGTGGCAGCTACAATTCCACCTACTGCGTCTGCCCTCCGGGATTCATCCCGGATTCTCGCGGCGGAGCACGGCAAGGATGCCACCGGAAACTCAAGGTATCAAAGAAAGTCAAGTTTCTTCCGCTCGATTTTGTGACTTTCAGAGGTGGGGTTAAAGAAATTTCCTTGCAACTCCCAAATATTTCGACGTGTGAAGCGAATTGCGTGAAGAATGCGAGCTGCGTTGGGTATACGTTCAGTTTCGATGGAAGCGCTGTGTGTGCGCTTCAGTTAGACATTTTGTCGAACGGGTTGTGGTCGCCCGGGATGAAGAAAGCTGCCTTTGTGAAGGTCGAAAATTCGGAAACTGATCGCTCAAACTTCACCGGAATGATGTACAGACTCGAATCGACGTGTCCGGTTCACATCACCCTCCGCCCGCCGCCGGAAGATAAAGACAACACCACCAGAAACGTACTGATAATCATCTCCATATTCGTGGCGGAACTAATTTCGGGGGCGGTTTTCTTCTGTGCATTCTTGAAGAGATTCGTAAAATACAGAGACATGGCTCGCACGCTCGGTCTGGAGTCGCTCCCCGCCGGCGGGCCGAAGCGGTTCACCTACGCCGAGCTGAAAACTGCCACCAACGACTTCTCGAACTCCATCGGAAGAGGCGGATTCGGCGAGGTCTTCAAAGGGGAGTTGCCGGATAAACGCGTGGTCGCCGTCAAGTGTTTGAAAAACGTTGCCGGCGGCGACGGCGACTTCTGGTCAGAGGTCACCATCATCGCCCGAATGCACCACCTCAACTTGCTCCGATTGTGGGGATTCTGCGCCGAAAAGGGTCAAAGAATGCTGGTCTACGAGTACATCCCCAATGGATCTCTCGACAAATTCCTGTTCATCAAACCGCAGCCCTCTGATTCGGAGACGGACGCAACAGAGACGGCCGGAGAAAACCCCTCACTCGATTGGAGCGTCCGGTACCGAATCGCCATAGGAGTGGCCAGAGCAATCGCGTATCTGCACGAGGAATGTCTGGAGTGGGTGTTACATCGAGACATAAAACCCGAAAACATCCTTCTGGACAACGATTTCTGCCCGAAACTATCGGATTTCGGGCTGTCGAAACTGAGGAAAAACGACGAGACGGCGGTGAGCATGTCTCGGATCAGAGGGACGCCGGGTTACGTGGCGCCGGAGCTGGTGAAATTGGGTTCGAATTCAATAACGACGAAGGCCGACGTGTACAGCTTCGGAATGGTGCTGCTGGAGATCATCAGCGGGACGAGGAACTTCGAGACGAAAAGATCGACGGTGGAGAGCGCGGATTGGTACTTTCCGGGATGGGCGTTCGAGAAAGCGTTCGTGGAGGAGAAGATCGAAGAGATTCTAGACCGTCGGATTAGGAATGAGTACGACAGCGGATCCCATTTTGCTATGGTAAATCGTATGGTTTCAACGGCGATGTGGTGCCTTCAGAACCAGCCGGAGATGCGGCCGCCGATGGGGAAAGTCGTGAAGATGTTGGAAGGAAAGCTCGAAATTCCTCCCCCGGATAAGCCCTCCATTTACTTCTTGTCAGAACCCCAAAATTAA

Protein sequence

MSLPLLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPSTNNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNNVFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNLTFGTATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSNLQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKLKVSKKVKFLPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQLDILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVEEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIPPPDKPSIYFLSEPQN
Homology
BLAST of MC04g_new0453 vs. ExPASy Swiss-Prot
Match: Q9XID3 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 OS=Arabidopsis thaliana OX=3702 GN=At1g34300 PE=1 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 3.8e-103
Identity = 286/825 (34.67%), Postives = 396/825 (48.00%), Query Frame = 0

Query: 3   LPLLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPST 62
           LPLL LL  F             P +FS   +    +    N    SPNS F+  F PS 
Sbjct: 11  LPLLLLLLHF-------------PFSFSTIPLGSVIYASGSNQNWPSPNSTFSVSFVPSP 70

Query: 63  NNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNNV 122
            + N ++ +V F   +    +WSA     V+   +L + +SG LRL +GSG  +W S   
Sbjct: 71  -SPNSFLAAVSF---AGSVPIWSA---GTVDSRGSLRLHTSGSLRLTNGSGTTVWDSKT- 130

Query: 123 FGNPNSTKLMLRDDGDLI------YATWESFQFPTNTILPNQTFNETTIVSNNGKYEF-- 182
                 T   + D G+ I         W SF  PT+TI+ +Q F    I+  +G Y F  
Sbjct: 131 -DRLGVTSGSIEDTGEFILLNNRSVPVWSSFDNPTDTIVQSQNFTAGKIL-RSGLYSFQL 190

Query: 183 KNSVNLTF---GTATYWNSG----------NPFNIFQTDGRI------IRNNQGPIFPSD 242
           + S NLT     +A YWN G          +P    QT+G +      +      ++  D
Sbjct: 191 ERSGNLTLRWNTSAIYWNHGLNSSFSSNLSSPRLSLQTNGVVSIFESNLLGGAEIVYSGD 250

Query: 243 F-NSTRLRKLVVDDDGNLKIFSFNS-NLQRWGVVWQAHVELCQIYATCGPNSICMSGGSY 302
           + +S   R L +DDDGNL+I+S  S N       W A V+ C +Y  CG   IC    SY
Sbjct: 251 YGDSNTFRFLKLDDDGNLRIYSSASRNSGPVNAHWSA-VDQCLVYGYCGNFGIC----SY 310

Query: 303 NST--YCVCPP-GFIPDSRGGARQGCHRKLKVSK-KVKFLPLDFV-----TFRGGVKEIS 362
           N T   C CP   F        R+GC RK+++S        LD V     T+       S
Sbjct: 311 NDTNPICSCPSRNFDFVDVNDRRKGCKRKVELSDCSGNTTMLDLVHTRLFTYEDDPNSES 370

Query: 363 LQLPNISTCEANCVKNASCVGYTFSFDGSAVC-ALQLDILSNGLWSPGMKKAAFVKVENS 422
                 S C ANC+ +  C+      DGS  C          G   P +   ++VKV   
Sbjct: 371 F-FAGSSPCRANCLSSVLCLASVSMSDGSGNCWQKHPGSFFTGYQWPSVPSTSYVKVCGP 430

Query: 423 ETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLIIISIFVAELISGAV------ 482
                              V  TL    +  DN ++  L I+++ V   + G V      
Sbjct: 431 ------------------VVANTLERATKGDDNNSKVHLWIVAVAVIAGLLGLVAVEIGL 490

Query: 483 FFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNSIGRGGFGEVFKGE 542
           ++C   K   ++  ++    L    +G P +FTY EL+  T  F   +G GGFG V++G 
Sbjct: 491 WWCCCRKN-PRFGTLSSHYTLLEYASGAPVQFTYKELQRCTKSFKEKLGAGGFGTVYRGV 550

Query: 543 LPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRMLVYEYIPNG 602
           L ++ VVAVK L+ +  G+  F  EV  I+  HHLNL+RL GFC++   R+LVYE++ NG
Sbjct: 551 LTNRTVVAVKQLEGIEQGEKQFRMEVATISSTHHLNLVRLIGFCSQGRHRLLVYEFMRNG 610

Query: 603 SLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAYLHEECLEWVLHRD 662
           SLD FLF              T      L W  R+ IA+G A+ I YLHEEC + ++H D
Sbjct: 611 SLDNFLF--------------TTDSAKFLTWEYRFNIALGTAKGITYLHEECRDCIVHCD 670

Query: 663 IKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKLGSNSITTKA 722
           IKPENIL+D++F  K+SDFGL+KL    +   +MS +RGT GY+APE   L +  IT+K+
Sbjct: 671 IKPENILVDDNFAAKVSDFGLAKLLNPKDNRYNMSSVRGTRGYLAPEW--LANLPITSKS 730

Query: 723 DVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVEEKIEEILDRRIRNEYD 782
           DVYS+GMVLLE++SG RNF+    T       F  WA+E+ F +   + ILD R+    D
Sbjct: 731 DVYSYGMVLLELVSGKRNFDVSEKTNHKK---FSIWAYEE-FEKGNTKAILDTRLSE--D 765

BLAST of MC04g_new0453 vs. ExPASy Swiss-Prot
Match: Q39203 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-2 OS=Arabidopsis thaliana OX=3702 GN=SD22 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.8e-98
Identity = 276/839 (32.90%), Postives = 403/839 (48.03%), Query Frame = 0

Query: 3   LPLLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPST 62
           LPLL LLF         PPP+            QS    + N  +LS  ++F  GF  +T
Sbjct: 7   LPLLLLLFLL-------PPPS-----------VQSKVIIKGNQTILSFKAIFRLGFFSTT 66

Query: 63  NNSNLYVFSVWFLNVSTDNVVWSANRLSPVN--RSAALAITSSGQL---RLNDG---SGR 122
           N S+ +   + + ++ T   VW ANR+ PV+   S+ L +TS+G L    L DG      
Sbjct: 67  NGSSNWYLGISYASMPTPTHVWVANRIRPVSDPDSSTLELTSTGYLIVSNLRDGVVWQTD 126

Query: 123 NLWPSNNVFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVS------- 182
           N  P  +   +     +++ DDG  +   W+SF  PT+T LP       T ++       
Sbjct: 127 NKQPGTDFRFSETGNLILINDDGSPV---WQSFDNPTDTWLPGMNVTGLTAMTSWRSLFD 186

Query: 183 -NNGKYEFK-----NSVNLTF-GTATYWNSGN-PFNIF-----QTDGRIIR-NNQGPIFP 242
            + G Y  +     N   L + GT  YW++GN     F      T   I R +   P  P
Sbjct: 187 PSPGFYSLRLSPSFNEFQLVYKGTTPYWSTGNWTGEAFVGVPEMTIPYIYRFHFVNPYTP 246

Query: 243 S-----------DFNSTRLRKLVVDDDGNLKIFSFNSNLQRWGVVWQAHVELCQIYATCG 302
           +             +  RL + +V  +G LK ++++   Q W + W    + C++Y  CG
Sbjct: 247 TASFWYIVPPLDSVSEPRLTRFMVGANGQLKQYTWDPQTQSWNMFWLQPEDPCRVYNLCG 306

Query: 303 PNSICMSGGSYNSTYCVCPPGFIPDSRGGAR-----QGCHRKLKVS--KKVKFLPLDFVT 362
               C    S     C C  GF P +    R      GC R+   S  K   F  +  + 
Sbjct: 307 QLGFC---SSELLKPCACIRGFRPRNDAAWRSDDYSDGCRRENGDSGEKSDTFEAVGDLR 366

Query: 363 FRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQLDILSNGLWSPGMKKA 422
           + G VK   LQ+   S+C   C+ N+SCVG+ +  + S +C + L+  +N          
Sbjct: 367 YDGDVKMSRLQVSK-SSCAKTCLGNSSCVGF-YHKEKSNLCKILLESPNN---------- 426

Query: 423 AFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLIIISIFVAELISG 482
               ++NS                              K N +++++I+ S+  +  + G
Sbjct: 427 ----LKNS------------------------------KGNISKSIIILCSVVGSISVLG 486

Query: 483 AVFF--CAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNSIGRGGFGEV 542
                    LKR  K R   R    +       K F++ EL++ATN FS+ +G GGFG V
Sbjct: 487 FTLLVPLILLKRSRK-RKKTRKQDEDGFAVLNLKVFSFKELQSATNGFSDKVGHGGFGAV 546

Query: 543 FKGELP-DKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRMLVYE 602
           FKG LP     VAVK L+    G+ +F +EV  I  + H+NL+RL GFC+E   R+LVY+
Sbjct: 547 FKGTLPGSSTFVAVKRLERPGSGESEFRAEVCTIGNIQHVNLVRLRGFCSENLHRLLVYD 606

Query: 603 YIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAYLHEECLEW 662
           Y+P GSL  +L        S T           L W  R+RIA+G A+ IAYLHE C + 
Sbjct: 607 YMPQGSLSSYL--------SRTSP-------KLLSWETRFRIALGTAKGIAYLHEGCRDC 666

Query: 663 VLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKLGSNS 722
           ++H DIKPENILLD+D+  K+SDFGL+KL   D + V ++ +RGT GYVAPE +      
Sbjct: 667 IIHCDIKPENILLDSDYNAKVSDFGLAKLLGRDFSRV-LATMRGTWGYVAPEWI--SGLP 726

Query: 723 ITTKADVYSFGMVLLEIISGTRNFETKRSTV-----ESADWYFPGWAFEKAFVEEKIEEI 782
           ITTKADVYSFGM LLE+I G RN      T+     E   W+FP WA  +  ++  ++ +
Sbjct: 727 ITTKADVYSFGMTLLELIGGRRNVIVNSDTLGEKETEPEKWFFPPWA-AREIIQGNVDSV 751

Query: 783 LDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLE--IPPPDK 785
           +D R+  EY++      V RM + A+WC+Q+  E+RP MG VVKMLEG +E  +PPP K
Sbjct: 787 VDSRLNGEYNTEE----VTRMATVAIWCIQDNEEIRPAMGTVVKMLEGVVEVTVPPPPK 751

BLAST of MC04g_new0453 vs. ExPASy Swiss-Prot
Match: Q9FLV4 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabidopsis thaliana OX=3702 GN=At5g24080 PE=2 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.4e-97
Identity = 246/831 (29.60%), Postives = 404/831 (48.62%), Query Frame = 0

Query: 42  EQNLILLSPNSLFAAGFRPSTNNSNLYVFSVWFLNVSTD-NVVWSANRLSPVNRSAALAI 101
           E N   +S N  FA GF      ++ ++ S+WF  +  D  +VWS NR SPV + A L +
Sbjct: 40  EPNRAWVSANGTFAIGF-TRFKPTDRFLLSIWFAQLPGDPTIVWSPNRNSPVTKEAVLEL 99

Query: 102 TSSGQLRLNDGSGRNLWPSNNVFGNPNSTKLMLRDDGDLIY---------ATWESFQFPT 161
            ++G L L+D     +W SN    N      ++ + G+ +            W+SF  P+
Sbjct: 100 EATGNLVLSD-QNTVVWTSNT--SNHGVESAVMSESGNFLLLGTEVTAGPTIWQSFSQPS 159

Query: 162 NTILPNQTFNETTIVSNN------GKYEFKN-------SVNLTF-------GTATYWNSG 221
           +T+LPNQ    +  +++N      G Y  K        S+ LT+          +YW+  
Sbjct: 160 DTLLPNQPLTVSLELTSNPSPSRHGHYSLKMLQQHTSLSLGLTYNINLDPHANYSYWSGP 219

Query: 222 NPFNIFQTDGRII-----------RNNQGPIF----PSDFN-------------STRLRK 281
           +  N+      ++            ++ G ++    P D N             +  LR+
Sbjct: 220 DISNVTGDVTAVLDDTGSFKIVYGESSIGAVYVYKNPVDDNRNYNNSSNLGLTKNPVLRR 279

Query: 282 LVVDDDGNLKIFSFNSNL---QRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVC 341
           LV++++GNL+++ +++++    +W   W A    C I   CG N +C    +  +  C+C
Sbjct: 280 LVLENNGNLRLYRWDNDMNGSSQWVPEWAAVSNPCDIAGICG-NGVCNLDRTKKNADCLC 339

Query: 342 PPGFI--PDSRGGAR--------QGCHRKLKVSKKVKFLPLD----FVTFRGGVKEISLQ 401
            PG +  PD              Q C   +  +   K   +     + + R  ++ IS  
Sbjct: 340 LPGSVKLPDQENAKLCSDNSSLVQECESNINRNGSFKISTVQETNYYFSERSVIENIS-D 399

Query: 402 LPNISTCEANCVKNASCVGYTFSFDG-SAVCALQLDILSNGLWSPGMKKAAFVKVENSET 461
           + N+  C   C+ +  CV   +  D     C +   +   G   PG     FVK   +E+
Sbjct: 400 ISNVRKCGEMCLSDCKCVASVYGLDDEKPYCWILKSLNFGGFRDPG--STLFVKTRANES 459

Query: 462 DRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLIIISIFVAELISGAVFFCAFLKR 521
             SN                        K +  R  +++I I V  L+  A+        
Sbjct: 460 YPSNSNN---------------NDSKSRKSHGLRQKVLVIPIVVGMLVLVALLGMLLYYN 519

Query: 522 FVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNSIGRGGFGEVFKGELPDKRVVA 581
             + R + R      +    P  FTY +L+  TN+FS  +G GGFG V+KG +  + +VA
Sbjct: 520 LDRKRTLKRAAKNSLILCDSPVSFTYRDLQNCTNNFSQLLGSGGFGTVYKGTVAGETLVA 579

Query: 582 VKCL-KNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRMLVYEYIPNGSLDKFLF 641
           VK L + ++ G+ +F +EV  I  MHH+NL+RL G+C+E   R+LVYEY+ NGSLDK++F
Sbjct: 580 VKRLDRALSHGEREFITEVNTIGSMHHMNLVRLCGYCSEDSHRLLVYEYMINGSLDKWIF 639

Query: 642 IKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAYLHEECLEWVLHRDIKPENIL 701
              Q ++              LDW  R+ IA+  A+ IAY HE+C   ++H DIKPENIL
Sbjct: 640 SSEQTAN-------------LLDWRTRFEIAVATAQGIAYFHEQCRNRIIHCDIKPENIL 699

Query: 702 LDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKLGSNSITTKADVYSFGM 761
           LD++FCPK+SDFGL+K+   + + V ++ IRGT GY+APE V   +  IT KADVYS+GM
Sbjct: 700 LDDNFCPKVSDFGLAKMMGREHSHV-VTMIRGTRGYLAPEWV--SNRPITVKADVYSYGM 759

Query: 762 VLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVEEKIEEILDRRIRNEYDSGSHFAM 793
           +LLEI+ G RN +    + ++ D+++PGWA+++      ++ + D+R++   +       
Sbjct: 760 LLLEIVGGRRNLD---MSYDAEDFFYPGWAYKELTNGTSLKAV-DKRLQGVAEEEE---- 819

BLAST of MC04g_new0453 vs. ExPASy Swiss-Prot
Match: P17801 (Putative receptor protein kinase ZmPK1 OS=Zea mays OX=4577 GN=PK1 PE=2 SV=2)

HSP 1 Score: 347.1 bits (889), Expect = 5.5e-94
Identity = 255/796 (32.04%), Postives = 392/796 (49.25%), Query Frame = 0

Query: 47  LLSPNSLFAAGFRPSTNNSNLYVFSVWFLN-----VSTDNVVWSANRLSPVN-RSAALAI 106
           L S +  F++GF     ++  + FSVW+        +   +VWSAN   PV+ R +AL +
Sbjct: 48  LQSSDGTFSSGFYEVYTHA--FTFSVWYSKTEAAAANNKTIVWSANPDRPVHARRSALTL 107

Query: 107 TSSGQLRLNDGSGRNLWPSN-NVFGNPNSTKLMLRDDGDLIY------ATWESFQFPTNT 166
              G + L D  G  +W ++ N F      +L+  D G+L+         W+SF  PT+T
Sbjct: 108 QKDGNMVLTDYDGAAVWRADGNNFTGVQRARLL--DTGNLVIEDSGGNTVWQSFDSPTDT 167

Query: 167 ILPNQTFN------ETTIVSNNGKYEFKNS----VNLTFG----TATYWNSGNPFNIFQT 226
            LP Q          TT   + G Y F+ S    ++L +     +  YW   +  N++Q 
Sbjct: 168 FLPTQLITAATRLVPTTQSRSPGNYIFRFSDLSVLSLIYHVPQVSDIYWPDPDQ-NLYQ- 227

Query: 227 DGRIIRN--------NQGPIFPSDFNSTRL-----------RKLVVDDDGNLKIFSFNSN 286
           DGR   N        + G +  SDF   +            R+L +D DGNL+++S N +
Sbjct: 228 DGRNQYNSTRLGMLTDSGVLASSDFADGQALVASDVGPGVKRRLTLDPDGNLRLYSMNDS 287

Query: 287 LQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNST-YCVCPPGFIPDSRGGARQGCHRK 346
              W V   A  + C I+  CGPN IC     Y+ T  C CPPG+   + G   +GC   
Sbjct: 288 DGSWSVSMVAMTQPCNIHGLCGPNGIC----HYSPTPTCSCPPGYATRNPGNWTEGCMAI 347

Query: 347 LKVS------KKVKFLPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGS 406
           +  +      + ++F+ L    F G  ++  L + ++ TC   C+ + +C G+ +  +G+
Sbjct: 348 VNTTCDRYDKRSMRFVRLPNTDFWGSDQQHLLSV-SLRTCRDICISDCTCKGFQYQ-EGT 407

Query: 407 AVCALQLDILSNGLWSPG------MKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITL 466
             C  +  + S   +         +K    V V N+   RS+    + R      ++ ++
Sbjct: 408 GSCYPKAYLFSGRTYPTSDVRTIYLKLPTGVSVSNALIPRSDVFDSVPRRLDCDRMNKSI 467

Query: 467 RPPPEDKDNTTRNVL-------IIISIFVAEL--ISGAVFFCAFLKRFVKYRDM-ARTLG 526
           R P  D   T             I + FV E+  IS A FF   LKR ++  ++ A   G
Sbjct: 468 REPFPDVHKTGGGESKWFYFYGFIAAFFVVEVSFISFAWFF--VLKRELRPSELWASEKG 527

Query: 527 LESLPAGGPKRFTYAELKTATNDFSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDG 586
            +++ +   +R++Y EL  AT  F   +GRG  G V+KG L D R VAVK L+NV  G  
Sbjct: 528 YKAMTSNF-RRYSYRELVKATRKFKVELGRGESGTVYKGVLEDDRHVAVKKLENVRQGKE 587

Query: 587 DFWSEVTIIARMHHLNLLRLWGFCAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDAT 646
            F +E+++I R++H+NL+R+WGFC+E   R+LV EY+ NGSL   LF             
Sbjct: 588 VFQAELSVIGRINHMNLVRIWGFCSEGSHRLLVSEYVENGSLANILF------------- 647

Query: 647 ETAGENPSLDWSVRYRIAIGVARAIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFG 706
            + G N  LDW  R+ IA+GVA+ +AYLH ECLEWV+H D+KPENILLD  F PK++DFG
Sbjct: 648 -SEGGNILLDWEGRFNIALGVAKGLAYLHHECLEWVIHCDVKPENILLDQAFEPKITDFG 707

Query: 707 LSKLRKNDETAVSMSRIRGTPGYVAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFE 766
           L KL     +  ++S +RGT GY+APE V   S  IT K DVYS+G+VLLE+++GTR  E
Sbjct: 708 LVKLLNRGGSTQNVSHVRGTLGYIAPEWV--SSLPITAKVDVYSYGVVLLELLTGTRVSE 767

Query: 767 TKRSTVESADWYFPGWAFEKAFVEEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQ 774
               T E             A +E + +  +D  + ++ +   ++     ++  A+ CL+
Sbjct: 768 LVGGTDEVHSMLRKLVRMLSAKLEGEEQSWIDGYLDSKLNRPVNYVQARTLIKLAVSCLE 812

BLAST of MC04g_new0453 vs. ExPASy Swiss-Prot
Match: O64477 (G-type lectin S-receptor-like serine/threonine-protein kinase At2g19130 OS=Arabidopsis thaliana OX=3702 GN=At2g19130 PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.7e-93
Identity = 248/805 (30.81%), Postives = 395/805 (49.07%), Query Frame = 0

Query: 47  LLSPNSLFAAGFRPSTNNSNLYVFSVWFLNVSTDNVVWSANRLSPV-NRSAALAITSSGQ 106
           ++S +  +  GF    ++SN Y+  +W+  +S   ++W ANR   V ++++++   S+G 
Sbjct: 38  IVSSDGTYEMGFFKPGSSSNFYI-GMWYKQLS-QTILWVANRDKAVSDKNSSVFKISNGN 97

Query: 107 LRLNDGSGRN-LWPSN-NVFGNPNSTKLMLRDDGDLIYAT----------WESFQFPTNT 166
           L L DG+ +  +W +  N   + ++ + +L+DDG+L+  T          W+SF  P +T
Sbjct: 98  LILLDGNYQTPVWSTGLNSTSSVSALEAVLQDDGNLVLRTGGSSLSANVLWQSFDHPGDT 157

Query: 167 ILP-------NQTFNETTIVS-------NNGKYEFKNSVNLTF-----GTATYWNSGNPF 226
            LP        +T     + S       + G +  +   +  +     G+  YW+SG P+
Sbjct: 158 WLPGVKIRLDKRTGKSQRLTSWKSLEDPSPGLFSLELDESTAYKILWNGSNEYWSSG-PW 217

Query: 227 N----IFQT----------DGRIIRNNQGPIFP-SDFNSTRLRKLVVDDDGNLKIFSFNS 286
           N    IF +          +     N     F  S +N   + + V+D  G +K F++  
Sbjct: 218 NPQSRIFDSVPEMRLNYIYNFSFFSNTTDSYFTYSIYNQLNVSRFVMDVSGQIKQFTWLE 277

Query: 287 NLQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSR-----GGARQ 346
             + W + W    + CQ+Y  CG   IC      +  +C CP GF P S+          
Sbjct: 278 GNKAWNLFWSQPRQQCQVYRYCGSFGIC---SDKSEPFCRCPQGFRPMSQKDWDLKDYSA 337

Query: 347 GCHRKLKVSKKVKFLPLDFVTFRGGVKEISLQLPN--------------ISTCEANCVKN 406
           GC RK ++              RG + +   +LPN              +S C + C  +
Sbjct: 338 GCVRKTELQ-----------CSRGDINQF-FRLPNMKLADNSEVLTRTSLSICASACQGD 397

Query: 407 ASCVGYTFSFDGSAVCALQLDILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLEST 466
            SC  Y +  +GS+ C          +WS  +     ++ ENSE +         RL ++
Sbjct: 398 CSCKAYAYD-EGSSKCL---------VWSKDVLNLQQLEDENSEGNI-----FYLRLAAS 457

Query: 467 CPVHITLRPPPEDKDNTTRNVLIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLES 526
              ++       +K      VL  + + V  L+         +   ++YR   R  G + 
Sbjct: 458 DVPNVGASGKSNNKGLIFGAVLGSLGVIVLVLL--------VVILILRYRRRKRMRGEKG 517

Query: 527 LPAGGPKRFTYAELKTATNDFSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFW 586
              G    F+Y EL+ AT +FS+ +G GGFG VFKG LPD   +AVK L+ ++ G+  F 
Sbjct: 518 --DGTLSAFSYRELQNATKNFSDKLGGGGFGSVFKGALPDSSDIAVKRLEGISQGEKQFR 577

Query: 587 SEVTIIARMHHLNLLRLWGFCAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETA 646
           +EV  I  + H+NL+RL GFC+E  +++LVY+Y+PNGSLD  LF+               
Sbjct: 578 TEVVTIGTIQHVNLVRLRGFCSEGSKKLLVYDYMPNGSLDSHLFL------------NQV 637

Query: 647 GENPSLDWSVRYRIAIGVARAIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSK 706
            E   L W +R++IA+G AR +AYLH+EC + ++H DIKPENILLD+ FCPK++DFGL+K
Sbjct: 638 EEKIVLGWKLRFQIALGTARGLAYLHDECRDCIIHCDIKPENILLDSQFCPKVADFGLAK 697

Query: 707 LRKNDETAVSMSRIRGTPGYVAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKR 766
           L   D + V ++ +RGT GY+APE +     +IT KADVYS+GM+L E++SG RN  T++
Sbjct: 698 LVGRDFSRV-LTTMRGTRGYLAPEWI--SGVAITAKADVYSYGMMLFELVSGRRN--TEQ 757

Query: 767 STVESADWYFPGWAFEKAFVEEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQP 786
           S  E    +FP WA      +  I  ++D R+  +         V R    A WC+Q++ 
Sbjct: 758 SENEKVR-FFPSWAATILTKDGDIRSLVDPRLEGD---AVDIEEVTRACKVACWCIQDEE 778

BLAST of MC04g_new0453 vs. NCBI nr
Match: XP_022146471.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 [Momordica charantia])

HSP 1 Score: 1609 bits (4166), Expect = 0.0
Identity = 788/795 (99.12%), Postives = 790/795 (99.37%), Query Frame = 0

Query: 1   MSLPLLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRP 60
           MSLPLLCLLFFFTSPAAAQPPPA KPRNFSAFSISQSPWRPEQNLILLSPNSLFA GFRP
Sbjct: 28  MSLPLLCLLFFFTSPAAAQPPPAXKPRNFSAFSISQSPWRPEQNLILLSPNSLFAXGFRP 87

Query: 61  STNNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSN 120
           STNNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSG NLWPSN
Sbjct: 88  STNNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGXNLWPSN 147

Query: 121 NVFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNL 180
           NVFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNL
Sbjct: 148 NVFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNL 207

Query: 181 TFGTATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSN 240
           TFGTATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSN
Sbjct: 208 TFGTATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSN 267

Query: 241 LQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKL 300
           LQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKL
Sbjct: 268 LQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKL 327

Query: 301 KVSKKVKFLPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQL 360
           KVSKKVKFLPLDFVTFRGGV+EISLQ+ NIS CEANCVKNASCVGYTFSFDGSAVCALQL
Sbjct: 328 KVSKKVKFLPLDFVTFRGGVREISLQVMNISMCEANCVKNASCVGYTFSFDGSAVCALQL 387

Query: 361 DILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRN 420
           DILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRN
Sbjct: 388 DILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRN 447

Query: 421 VLIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATND 480
           VLIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATND
Sbjct: 448 VLIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATND 507

Query: 481 FSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGF 540
           FSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGF
Sbjct: 508 FSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGF 567

Query: 541 CAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVAR 600
           CAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVAR
Sbjct: 568 CAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVAR 627

Query: 601 AIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGY 660
           AIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGY
Sbjct: 628 AIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGY 687

Query: 661 VAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFV 720
           VAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFV
Sbjct: 688 VAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFV 747

Query: 721 EEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIP 780
           EEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIP
Sbjct: 748 EEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIP 807

Query: 781 PPDKPSIYFLSEPQN 795
           PPDKPSIYFLSEPQN
Sbjct: 808 PPDKPSIYFLSEPQN 822

BLAST of MC04g_new0453 vs. NCBI nr
Match: XP_038875239.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 [Benincasa hispida])

HSP 1 Score: 1377 bits (3564), Expect = 0.0
Identity = 670/793 (84.49%), Postives = 720/793 (90.79%), Query Frame = 0

Query: 4   PLLCLLFFFTSP---AAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRP 63
           PL  LLFFFTSP   AAAQP     PRNFSAFSISQSPWRP QNLILLSPNS FAAGFRP
Sbjct: 7   PLSILLFFFTSPPSLAAAQP--TANPRNFSAFSISQSPWRPTQNLILLSPNSHFAAGFRP 66

Query: 64  STNNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSN 123
             NNSNL++FSVW+ N+S DNVVWSANRLSPVNRSAAL IT++GQLRLNDGSGRNLWPSN
Sbjct: 67  LPNNSNLFIFSVWYFNISIDNVVWSANRLSPVNRSAALVITATGQLRLNDGSGRNLWPSN 126

Query: 124 NVFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNL 183
           +V  NPN TKL+LR+DGDLIY TWESF+FPTNTILPNQT N TTIVSNNGKY F NSVNL
Sbjct: 127 HVSANPNLTKLILRNDGDLIYGTWESFRFPTNTILPNQTLNGTTIVSNNGKYSFVNSVNL 186

Query: 184 TFGTATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSN 243
           TFGT  YW SGNPF  F   G+II NNQ P+ PSDFNSTRLRKL+VDDDGNLKIFSFN N
Sbjct: 187 TFGTERYWTSGNPFQTFLIGGQIIINNQIPVTPSDFNSTRLRKLIVDDDGNLKIFSFNPN 246

Query: 244 LQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKL 303
             RW VVWQAHVELCQI+ TCGPNS+CMS GSYNSTYCVC PGF PDSRGGAR+GCHRKL
Sbjct: 247 SSRWDVVWQAHVELCQIFDTCGPNSVCMSSGSYNSTYCVCAPGFSPDSRGGARRGCHRKL 306

Query: 304 KVSKKVKFLPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQL 363
            VS K KFL LDFV+FRGG K+ISLQ PNIS C+A+C+KN+SCVGYTFSFDGSA C LQL
Sbjct: 307 NVSNKPKFLQLDFVSFRGGAKQISLQTPNISVCQADCLKNSSCVGYTFSFDGSAQCVLQL 366

Query: 364 DILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRN 423
           DILSNGLWSPGMK AAFVKV+NSETD+SNFTGMMY+L++TCPV ITLRPPP +KDNTTRN
Sbjct: 367 DILSNGLWSPGMKTAAFVKVDNSETDQSNFTGMMYKLQTTCPVRITLRPPPVNKDNTTRN 426

Query: 424 VLIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATND 483
           +LII +IFVAELI+GAVFF AFLKRF+KYRDMARTLGLESLPAGGPKRF YAELKTATND
Sbjct: 427 ILIISTIFVAELITGAVFFYAFLKRFIKYRDMARTLGLESLPAGGPKRFNYAELKTATND 486

Query: 484 FSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGF 543
           FSN IG+GGFG+VFKGELPDKRVVAVKCLKNVAGGD DFW+EVTIIARMHHLNLLRLWGF
Sbjct: 487 FSNCIGKGGFGKVFKGELPDKRVVAVKCLKNVAGGDRDFWAEVTIIARMHHLNLLRLWGF 546

Query: 544 CAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVAR 603
           CAEKGQRMLVYE+IPNGSLDKFLF+K  PSDSE D TET  ++PSLDWS+RYRIAIGVAR
Sbjct: 547 CAEKGQRMLVYEHIPNGSLDKFLFVKASPSDSEKDGTETEEQSPSLDWSIRYRIAIGVAR 606

Query: 604 AIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGY 663
           AIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKN+ETAVSMSRIRGTPGY
Sbjct: 607 AIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNEETAVSMSRIRGTPGY 666

Query: 664 VAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFV 723
           VAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFE KRSTVESADWYFPGWAFEKAFV
Sbjct: 667 VAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFEIKRSTVESADWYFPGWAFEKAFV 726

Query: 724 EEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIP 783
           EEK++EILD R+R+EY+SG+HFA++NRMV TAMWCLQ+QPE RP MGKVVKMLEGKLEIP
Sbjct: 727 EEKMKEILDVRLRDEYESGAHFAIINRMVQTAMWCLQSQPEKRPSMGKVVKMLEGKLEIP 786

Query: 784 PPDKPSIYFLSEP 793
           PP KPSIYFLSEP
Sbjct: 787 PPQKPSIYFLSEP 797

BLAST of MC04g_new0453 vs. NCBI nr
Match: XP_038906503.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 [Benincasa hispida])

HSP 1 Score: 1358 bits (3515), Expect = 0.0
Identity = 657/786 (83.59%), Postives = 711/786 (90.46%), Query Frame = 0

Query: 9   LFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPSTNNSNLY 68
           L FF SP  AQPP   KPRNFSAFSISQSPWRP QNLILLSPNSLFAAGFRP  NNSNL+
Sbjct: 12  LLFFPSPVTAQPP---KPRNFSAFSISQSPWRPTQNLILLSPNSLFAAGFRPLPNNSNLF 71

Query: 69  VFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNNVFGNPNS 128
           +FSVW+ N+STDNVVWSANRLSPVNRSAALAIT++GQLRLNDGSGRNLWPSN+V  NPNS
Sbjct: 72  IFSVWYFNISTDNVVWSANRLSPVNRSAALAITATGQLRLNDGSGRNLWPSNSVSANPNS 131

Query: 129 TKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNLTFGTATYW 188
           T+L+LR+DGDLIY TWESFQFPTNTILPNQT N TTIVSNNGKY F NSVNLTFGT  YW
Sbjct: 132 TQLILRNDGDLIYGTWESFQFPTNTILPNQTLNGTTIVSNNGKYSFVNSVNLTFGTERYW 191

Query: 189 NSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSNLQRWGVVW 248
            + NPF  F+  G I R+NQ PI+PSDFNSTR+RKLVVDDDGNLKIFS N N  RW VVW
Sbjct: 192 WTDNPFKNFENTGIINRDNQNPIYPSDFNSTRIRKLVVDDDGNLKIFSLNPNSPRWDVVW 251

Query: 249 QAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKLKVSKKVKF 308
           QAHVELC I+ TCGPNS+CMS GSYNSTYCVC PGF PD RGGAR+GCHRKL VS K KF
Sbjct: 252 QAHVELCHIFDTCGPNSVCMSTGSYNSTYCVCAPGFSPDPRGGARRGCHRKLNVSSKPKF 311

Query: 309 LPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQLDILSNGLW 368
           L LDFV+FRGGVK+ISLQ PNIS C+A+C+KN+SCVGYTFSFDGSA C LQLDILSNGLW
Sbjct: 312 LQLDFVSFRGGVKQISLQTPNISVCQADCLKNSSCVGYTFSFDGSAQCVLQLDILSNGLW 371

Query: 369 SPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLIIISIF 428
           SPGMK AAFVKV+NSETDRSNFTGMMY+L++TCPVHI+LRPPPE+KDNTTRN+ II++IF
Sbjct: 372 SPGMKTAAFVKVDNSETDRSNFTGMMYKLQTTCPVHISLRPPPENKDNTTRNIWIIVTIF 431

Query: 429 VAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNSIGRG 488
           VAELISGAVFFCAFLKRF+KYRDMARTLGLESLPAGGPKRF+Y ELK ATN+FSN +G+G
Sbjct: 432 VAELISGAVFFCAFLKRFIKYRDMARTLGLESLPAGGPKRFSYDELKIATNNFSNCVGKG 491

Query: 489 GFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRM 548
           GFGEVFKGELPDKR++AVKCLKNV+GGDGDFW+EVTIIARMHHLNLLRLWGFCAEKGQRM
Sbjct: 492 GFGEVFKGELPDKRIIAVKCLKNVSGGDGDFWAEVTIIARMHHLNLLRLWGFCAEKGQRM 551

Query: 549 LVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAYLHEE 608
           LVYEYI NGSLDKFLF+KP PSDS      T GE PSLDW +RYRIAIGVARAIAYLHEE
Sbjct: 552 LVYEYIANGSLDKFLFVKPPPSDS------TDGEIPSLDWGIRYRIAIGVARAIAYLHEE 611

Query: 609 CLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKL 668
           CLEWVLHRDIKPENILLDNDFCPKLSDFGLSKL+KNDETAVSMSRIRGTPGYVAPELVKL
Sbjct: 612 CLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLKKNDETAVSMSRIRGTPGYVAPELVKL 671

Query: 669 GSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVEEKIEEIL 728
           GS+SITTKADVYSFGMVLLEI+SGTRNF+TK STVESA WYFP WAFEKAFVE KIEE+L
Sbjct: 672 GSHSITTKADVYSFGMVLLEIVSGTRNFDTKGSTVESAFWYFPSWAFEKAFVEAKIEEVL 731

Query: 729 DRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIPPPDKPSIY 788
           D RIRN+YDSG+HFA+VNRMV TAMWC+QNQPE RP MGKVVKMLEGKLEIP P+KP IY
Sbjct: 732 DSRIRNQYDSGAHFAIVNRMVQTAMWCVQNQPEKRPAMGKVVKMLEGKLEIPLPEKPFIY 788

Query: 789 FLSEPQ 794
           FLSE Q
Sbjct: 792 FLSEGQ 788

BLAST of MC04g_new0453 vs. NCBI nr
Match: XP_008467083.1 (PREDICTED: G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 [Cucumis melo])

HSP 1 Score: 1351 bits (3496), Expect = 0.0
Identity = 658/788 (83.50%), Postives = 702/788 (89.09%), Query Frame = 0

Query: 5   LLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPSTNN 64
           L+ +L    S AAAQP     P NFS+FSISQSPWRP QNLILLSPNSLFAAGF P  NN
Sbjct: 11  LILILLLLLSLAAAQP--TTNPHNFSSFSISQSPWRPTQNLILLSPNSLFAAGFHPLPNN 70

Query: 65  SNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNNVFG 124
           SNL++FSVW+ N+STDNVVWSANRL PVNRSAAL IT++GQLRLND SGRNLWPSNNV  
Sbjct: 71  SNLFIFSVWYFNISTDNVVWSANRLHPVNRSAALVITATGQLRLNDPSGRNLWPSNNVSA 130

Query: 125 NPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNLTFGT 184
           N NST+L+LRDDGDLIY TWESFQFPTNT LPN TFN TTIVSNN KY F NS NLTFGT
Sbjct: 131 NSNSTQLILRDDGDLIYGTWESFQFPTNTFLPNHTFNGTTIVSNNRKYSFVNSANLTFGT 190

Query: 185 ATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSNLQRW 244
            TYW SGNPF  FQ DG+II NNQ P+ PSDFNSTRLRKLVVDDDGNLKIFSFN N  RW
Sbjct: 191 ETYWTSGNPFQNFQIDGQIIINNQIPVIPSDFNSTRLRKLVVDDDGNLKIFSFNPNWLRW 250

Query: 245 GVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKLKVSK 304
            VVWQAHVELC I  TCGPNS+CMS GSYNSTYCVC PGF PD RGGARQGCHRKL VS 
Sbjct: 251 DVVWQAHVELCHILDTCGPNSVCMSSGSYNSTYCVCAPGFSPDPRGGARQGCHRKLNVSS 310

Query: 305 KVKFLPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQLDILS 364
           K KFL LDFV FRGGVK+ISLQ PNIS C+A+C+KN+SCVGYTFSFDGSA C LQLDILS
Sbjct: 311 KAKFLQLDFVNFRGGVKQISLQTPNISVCQADCLKNSSCVGYTFSFDGSAQCVLQLDILS 370

Query: 365 NGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLII 424
           NGLWSPGMK AAFVKV+NSETDRSNFTGMMY+L++TCPV IT+RPPP +KDNTTRN+LII
Sbjct: 371 NGLWSPGMKAAAFVKVDNSETDRSNFTGMMYKLQTTCPVRITIRPPPVNKDNTTRNILII 430

Query: 425 ISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNS 484
            +IFVAELI+GAVFF AFLKRFVKYRDMARTLGLESLPAGGPKRF YAELKTATNDFS  
Sbjct: 431 STIFVAELITGAVFFWAFLKRFVKYRDMARTLGLESLPAGGPKRFNYAELKTATNDFSTC 490

Query: 485 IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEK 544
           IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGD DFW+EVTIIARMHHLNLLRLWGFCAEK
Sbjct: 491 IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDRDFWAEVTIIARMHHLNLLRLWGFCAEK 550

Query: 545 GQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAY 604
           GQR+LVYE+IPNGSLDKFLF+K  PSDSE D  ET  ++  LDWS+RYRIAIGVARAIAY
Sbjct: 551 GQRILVYEHIPNGSLDKFLFVKHSPSDSEKDERETEEQSSPLDWSIRYRIAIGVARAIAY 610

Query: 605 LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPE 664
           LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKN++T VSMSRIRGTPGYVAPE
Sbjct: 611 LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNEDTTVSMSRIRGTPGYVAPE 670

Query: 665 LVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVEEKI 724
           LVKLGSNSITTKADVYSFGMVLLEIISGTRNFE KRSTVESADWYFPGWAFEKAFVEEK+
Sbjct: 671 LVKLGSNSITTKADVYSFGMVLLEIISGTRNFEIKRSTVESADWYFPGWAFEKAFVEEKM 730

Query: 725 EEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIPPPDK 784
           +EILD RIR EY+SG H ++VNRMV TAMWC+QNQPE RP MGKVVKMLEGKLEIPPP+K
Sbjct: 731 KEILDGRIREEYESGGHVSIVNRMVQTAMWCVQNQPEKRPSMGKVVKMLEGKLEIPPPEK 790

Query: 785 PSIYFLSE 792
           PSIYFLSE
Sbjct: 791 PSIYFLSE 796

BLAST of MC04g_new0453 vs. NCBI nr
Match: KAA0046397.1 (G-type lectin S-receptor-like serine/threonine-protein kinase [Cucumis melo var. makuwa] >TYK14664.1 G-type lectin S-receptor-like serine/threonine-protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 657/788 (83.38%), Postives = 701/788 (88.96%), Query Frame = 0

Query: 5   LLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPSTNN 64
           L+ +L    S AAAQP     P NFS+FSISQSPWRP QNLILLSPNSLFAAGF P  NN
Sbjct: 73  LILILLLLLSLAAAQP--TTNPHNFSSFSISQSPWRPTQNLILLSPNSLFAAGFHPLPNN 132

Query: 65  SNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNNVFG 124
           SNL++FSVW+ N+STDNVVWSANRL PVNRSAAL IT++GQLRLND SGRNLWPSNNV  
Sbjct: 133 SNLFIFSVWYFNISTDNVVWSANRLHPVNRSAALVITATGQLRLNDPSGRNLWPSNNVSA 192

Query: 125 NPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNLTFGT 184
           N NST+L+LRDDGDLIY TWESFQFPTNT LPN TFN TTIVSNN KY F NS NLTFGT
Sbjct: 193 NSNSTQLILRDDGDLIYGTWESFQFPTNTFLPNHTFNGTTIVSNNRKYSFVNSANLTFGT 252

Query: 185 ATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSNLQRW 244
            TYW SGNPF  FQ DG+II NNQ P+ PSDFNSTRLRKLVVDDDGNLKIFSFN N  RW
Sbjct: 253 ETYWTSGNPFQNFQIDGQIIINNQIPVIPSDFNSTRLRKLVVDDDGNLKIFSFNPNWLRW 312

Query: 245 GVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKLKVSK 304
            VVWQAHVELC I  TCGPNS+CMS GSYNSTYCVC PGF PD RGGARQGCHRKL VS 
Sbjct: 313 DVVWQAHVELCHILDTCGPNSVCMSSGSYNSTYCVCAPGFSPDPRGGARQGCHRKLNVSS 372

Query: 305 KVKFLPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQLDILS 364
           K KFL LDFV FRGGVK+ISLQ PNIS C+A+C+KN+SCVGYTFSFDGSA C LQLDILS
Sbjct: 373 KAKFLQLDFVNFRGGVKQISLQTPNISVCQADCLKNSSCVGYTFSFDGSAQCVLQLDILS 432

Query: 365 NGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLII 424
           NGLWSPGMK AAFVKV+NSETDRSNFTGMMY+L++TCPV IT+RPPP +KDNTTRN+LII
Sbjct: 433 NGLWSPGMKAAAFVKVDNSETDRSNFTGMMYKLQTTCPVRITIRPPPVNKDNTTRNILII 492

Query: 425 ISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNS 484
            +IFVAELI+GAVFF AFLKRFVKYRDMARTLGLESLPAGGPKRF YAELKTATNDFS  
Sbjct: 493 STIFVAELITGAVFFWAFLKRFVKYRDMARTLGLESLPAGGPKRFNYAELKTATNDFSTC 552

Query: 485 IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEK 544
           IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGD DFW+EVTIIARMHHLNLLRLWGFCAEK
Sbjct: 553 IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDRDFWAEVTIIARMHHLNLLRLWGFCAEK 612

Query: 545 GQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAY 604
           GQR+LVYE+IPNGSLDKFLF+K  PSDS  D  ET  ++  LDWS+RYRIAIGVARAIAY
Sbjct: 613 GQRILVYEHIPNGSLDKFLFVKHSPSDSAKDERETEEQSSPLDWSIRYRIAIGVARAIAY 672

Query: 605 LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPE 664
           LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKN++T VSMSRIRGTPGYVAPE
Sbjct: 673 LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNEDTTVSMSRIRGTPGYVAPE 732

Query: 665 LVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVEEKI 724
           LVKLGSNSITTKADVYSFGMVLLEIISGTRNFE KRSTVESADWYFPGWAFEKAFVEEK+
Sbjct: 733 LVKLGSNSITTKADVYSFGMVLLEIISGTRNFEIKRSTVESADWYFPGWAFEKAFVEEKM 792

Query: 725 EEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIPPPDK 784
           +EILD RIR EY+SG H ++VNRMV TAMWC+QNQPE RP MGKVVKMLEGKLEIPPP+K
Sbjct: 793 KEILDGRIREEYESGGHVSIVNRMVQTAMWCVQNQPEKRPSMGKVVKMLEGKLEIPPPEK 852

Query: 785 PSIYFLSE 792
           PSIYFLSE
Sbjct: 853 PSIYFLSE 858

BLAST of MC04g_new0453 vs. ExPASy TrEMBL
Match: A0A6J1CZG1 (Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=LOC111015682 PE=3 SV=1)

HSP 1 Score: 1609 bits (4166), Expect = 0.0
Identity = 788/795 (99.12%), Postives = 790/795 (99.37%), Query Frame = 0

Query: 1   MSLPLLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRP 60
           MSLPLLCLLFFFTSPAAAQPPPA KPRNFSAFSISQSPWRPEQNLILLSPNSLFA GFRP
Sbjct: 28  MSLPLLCLLFFFTSPAAAQPPPAXKPRNFSAFSISQSPWRPEQNLILLSPNSLFAXGFRP 87

Query: 61  STNNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSN 120
           STNNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSG NLWPSN
Sbjct: 88  STNNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGXNLWPSN 147

Query: 121 NVFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNL 180
           NVFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNL
Sbjct: 148 NVFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNL 207

Query: 181 TFGTATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSN 240
           TFGTATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSN
Sbjct: 208 TFGTATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSN 267

Query: 241 LQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKL 300
           LQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKL
Sbjct: 268 LQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKL 327

Query: 301 KVSKKVKFLPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQL 360
           KVSKKVKFLPLDFVTFRGGV+EISLQ+ NIS CEANCVKNASCVGYTFSFDGSAVCALQL
Sbjct: 328 KVSKKVKFLPLDFVTFRGGVREISLQVMNISMCEANCVKNASCVGYTFSFDGSAVCALQL 387

Query: 361 DILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRN 420
           DILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRN
Sbjct: 388 DILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRN 447

Query: 421 VLIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATND 480
           VLIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATND
Sbjct: 448 VLIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATND 507

Query: 481 FSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGF 540
           FSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGF
Sbjct: 508 FSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGF 567

Query: 541 CAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVAR 600
           CAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVAR
Sbjct: 568 CAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVAR 627

Query: 601 AIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGY 660
           AIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGY
Sbjct: 628 AIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGY 687

Query: 661 VAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFV 720
           VAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFV
Sbjct: 688 VAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFV 747

Query: 721 EEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIP 780
           EEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIP
Sbjct: 748 EEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIP 807

Query: 781 PPDKPSIYFLSEPQN 795
           PPDKPSIYFLSEPQN
Sbjct: 808 PPDKPSIYFLSEPQN 822

BLAST of MC04g_new0453 vs. ExPASy TrEMBL
Match: A0A1S3CSP7 (Receptor-like serine/threonine-protein kinase OS=Cucumis melo OX=3656 GN=LOC103504518 PE=3 SV=1)

HSP 1 Score: 1351 bits (3496), Expect = 0.0
Identity = 658/788 (83.50%), Postives = 702/788 (89.09%), Query Frame = 0

Query: 5   LLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPSTNN 64
           L+ +L    S AAAQP     P NFS+FSISQSPWRP QNLILLSPNSLFAAGF P  NN
Sbjct: 11  LILILLLLLSLAAAQP--TTNPHNFSSFSISQSPWRPTQNLILLSPNSLFAAGFHPLPNN 70

Query: 65  SNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNNVFG 124
           SNL++FSVW+ N+STDNVVWSANRL PVNRSAAL IT++GQLRLND SGRNLWPSNNV  
Sbjct: 71  SNLFIFSVWYFNISTDNVVWSANRLHPVNRSAALVITATGQLRLNDPSGRNLWPSNNVSA 130

Query: 125 NPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNLTFGT 184
           N NST+L+LRDDGDLIY TWESFQFPTNT LPN TFN TTIVSNN KY F NS NLTFGT
Sbjct: 131 NSNSTQLILRDDGDLIYGTWESFQFPTNTFLPNHTFNGTTIVSNNRKYSFVNSANLTFGT 190

Query: 185 ATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSNLQRW 244
            TYW SGNPF  FQ DG+II NNQ P+ PSDFNSTRLRKLVVDDDGNLKIFSFN N  RW
Sbjct: 191 ETYWTSGNPFQNFQIDGQIIINNQIPVIPSDFNSTRLRKLVVDDDGNLKIFSFNPNWLRW 250

Query: 245 GVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKLKVSK 304
            VVWQAHVELC I  TCGPNS+CMS GSYNSTYCVC PGF PD RGGARQGCHRKL VS 
Sbjct: 251 DVVWQAHVELCHILDTCGPNSVCMSSGSYNSTYCVCAPGFSPDPRGGARQGCHRKLNVSS 310

Query: 305 KVKFLPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQLDILS 364
           K KFL LDFV FRGGVK+ISLQ PNIS C+A+C+KN+SCVGYTFSFDGSA C LQLDILS
Sbjct: 311 KAKFLQLDFVNFRGGVKQISLQTPNISVCQADCLKNSSCVGYTFSFDGSAQCVLQLDILS 370

Query: 365 NGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLII 424
           NGLWSPGMK AAFVKV+NSETDRSNFTGMMY+L++TCPV IT+RPPP +KDNTTRN+LII
Sbjct: 371 NGLWSPGMKAAAFVKVDNSETDRSNFTGMMYKLQTTCPVRITIRPPPVNKDNTTRNILII 430

Query: 425 ISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNS 484
            +IFVAELI+GAVFF AFLKRFVKYRDMARTLGLESLPAGGPKRF YAELKTATNDFS  
Sbjct: 431 STIFVAELITGAVFFWAFLKRFVKYRDMARTLGLESLPAGGPKRFNYAELKTATNDFSTC 490

Query: 485 IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEK 544
           IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGD DFW+EVTIIARMHHLNLLRLWGFCAEK
Sbjct: 491 IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDRDFWAEVTIIARMHHLNLLRLWGFCAEK 550

Query: 545 GQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAY 604
           GQR+LVYE+IPNGSLDKFLF+K  PSDSE D  ET  ++  LDWS+RYRIAIGVARAIAY
Sbjct: 551 GQRILVYEHIPNGSLDKFLFVKHSPSDSEKDERETEEQSSPLDWSIRYRIAIGVARAIAY 610

Query: 605 LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPE 664
           LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKN++T VSMSRIRGTPGYVAPE
Sbjct: 611 LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNEDTTVSMSRIRGTPGYVAPE 670

Query: 665 LVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVEEKI 724
           LVKLGSNSITTKADVYSFGMVLLEIISGTRNFE KRSTVESADWYFPGWAFEKAFVEEK+
Sbjct: 671 LVKLGSNSITTKADVYSFGMVLLEIISGTRNFEIKRSTVESADWYFPGWAFEKAFVEEKM 730

Query: 725 EEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIPPPDK 784
           +EILD RIR EY+SG H ++VNRMV TAMWC+QNQPE RP MGKVVKMLEGKLEIPPP+K
Sbjct: 731 KEILDGRIREEYESGGHVSIVNRMVQTAMWCVQNQPEKRPSMGKVVKMLEGKLEIPPPEK 790

Query: 785 PSIYFLSE 792
           PSIYFLSE
Sbjct: 791 PSIYFLSE 796

BLAST of MC04g_new0453 vs. ExPASy TrEMBL
Match: A0A5A7TWW0 (Receptor-like serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold663G00400 PE=3 SV=1)

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 657/788 (83.38%), Postives = 701/788 (88.96%), Query Frame = 0

Query: 5   LLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPSTNN 64
           L+ +L    S AAAQP     P NFS+FSISQSPWRP QNLILLSPNSLFAAGF P  NN
Sbjct: 73  LILILLLLLSLAAAQP--TTNPHNFSSFSISQSPWRPTQNLILLSPNSLFAAGFHPLPNN 132

Query: 65  SNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNNVFG 124
           SNL++FSVW+ N+STDNVVWSANRL PVNRSAAL IT++GQLRLND SGRNLWPSNNV  
Sbjct: 133 SNLFIFSVWYFNISTDNVVWSANRLHPVNRSAALVITATGQLRLNDPSGRNLWPSNNVSA 192

Query: 125 NPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNLTFGT 184
           N NST+L+LRDDGDLIY TWESFQFPTNT LPN TFN TTIVSNN KY F NS NLTFGT
Sbjct: 193 NSNSTQLILRDDGDLIYGTWESFQFPTNTFLPNHTFNGTTIVSNNRKYSFVNSANLTFGT 252

Query: 185 ATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSNLQRW 244
            TYW SGNPF  FQ DG+II NNQ P+ PSDFNSTRLRKLVVDDDGNLKIFSFN N  RW
Sbjct: 253 ETYWTSGNPFQNFQIDGQIIINNQIPVIPSDFNSTRLRKLVVDDDGNLKIFSFNPNWLRW 312

Query: 245 GVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKLKVSK 304
            VVWQAHVELC I  TCGPNS+CMS GSYNSTYCVC PGF PD RGGARQGCHRKL VS 
Sbjct: 313 DVVWQAHVELCHILDTCGPNSVCMSSGSYNSTYCVCAPGFSPDPRGGARQGCHRKLNVSS 372

Query: 305 KVKFLPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQLDILS 364
           K KFL LDFV FRGGVK+ISLQ PNIS C+A+C+KN+SCVGYTFSFDGSA C LQLDILS
Sbjct: 373 KAKFLQLDFVNFRGGVKQISLQTPNISVCQADCLKNSSCVGYTFSFDGSAQCVLQLDILS 432

Query: 365 NGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLII 424
           NGLWSPGMK AAFVKV+NSETDRSNFTGMMY+L++TCPV IT+RPPP +KDNTTRN+LII
Sbjct: 433 NGLWSPGMKAAAFVKVDNSETDRSNFTGMMYKLQTTCPVRITIRPPPVNKDNTTRNILII 492

Query: 425 ISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNS 484
            +IFVAELI+GAVFF AFLKRFVKYRDMARTLGLESLPAGGPKRF YAELKTATNDFS  
Sbjct: 493 STIFVAELITGAVFFWAFLKRFVKYRDMARTLGLESLPAGGPKRFNYAELKTATNDFSTC 552

Query: 485 IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEK 544
           IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGD DFW+EVTIIARMHHLNLLRLWGFCAEK
Sbjct: 553 IGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDRDFWAEVTIIARMHHLNLLRLWGFCAEK 612

Query: 545 GQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAY 604
           GQR+LVYE+IPNGSLDKFLF+K  PSDS  D  ET  ++  LDWS+RYRIAIGVARAIAY
Sbjct: 613 GQRILVYEHIPNGSLDKFLFVKHSPSDSAKDERETEEQSSPLDWSIRYRIAIGVARAIAY 672

Query: 605 LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPE 664
           LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKN++T VSMSRIRGTPGYVAPE
Sbjct: 673 LHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNEDTTVSMSRIRGTPGYVAPE 732

Query: 665 LVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVEEKI 724
           LVKLGSNSITTKADVYSFGMVLLEIISGTRNFE KRSTVESADWYFPGWAFEKAFVEEK+
Sbjct: 733 LVKLGSNSITTKADVYSFGMVLLEIISGTRNFEIKRSTVESADWYFPGWAFEKAFVEEKM 792

Query: 725 EEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIPPPDK 784
           +EILD RIR EY+SG H ++VNRMV TAMWC+QNQPE RP MGKVVKMLEGKLEIPPP+K
Sbjct: 793 KEILDGRIREEYESGGHVSIVNRMVQTAMWCVQNQPEKRPSMGKVVKMLEGKLEIPPPEK 852

Query: 785 PSIYFLSE 792
           PSIYFLSE
Sbjct: 853 PSIYFLSE 858

BLAST of MC04g_new0453 vs. ExPASy TrEMBL
Match: A0A6J1HG24 (Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111463621 PE=3 SV=1)

HSP 1 Score: 1348 bits (3488), Expect = 0.0
Identity = 654/786 (83.21%), Postives = 707/786 (89.95%), Query Frame = 0

Query: 9   LFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPSTNNSNLY 68
           L FFTSPAA+Q PP   PRNFSAFSISQSPWRP QNL+LLSPNSLFAAGFR    NSNL+
Sbjct: 12  LLFFTSPAASQSPP--NPRNFSAFSISQSPWRPTQNLLLLSPNSLFAAGFRQLPGNSNLF 71

Query: 69  VFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNNVFGNPNS 128
           +FSVW+ N+STD VVWSANRLSPVNRSAAL IT+SGQL L+DGSGRNLWPSN V  N NS
Sbjct: 72  LFSVWYFNISTDAVVWSANRLSPVNRSAALTITASGQLHLDDGSGRNLWPSNAVSVNSNS 131

Query: 129 TKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNLTFGTATYW 188
           TKL+LR+DGDLIYATWESFQFPTNTILPNQT N TTIVSNNGKY F+ SVNLTF    YW
Sbjct: 132 TKLILRNDGDLIYATWESFQFPTNTILPNQTLNGTTIVSNNGKYAFEKSVNLTFDKLMYW 191

Query: 189 NSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSNLQRWGVVW 248
           NSGNPF  F+ +G+I R+NQ  I+P+D+N TRLRKLVVDDDGNLKIFSFN   +RW VVW
Sbjct: 192 NSGNPFKDFENNGKINRDNQNAIYPNDYNMTRLRKLVVDDDGNLKIFSFNPIPRRWDVVW 251

Query: 249 QAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKLKVSKKVKF 308
           QAHVELCQIY TCG NSICMS GSYNSTYCVC PGF PD RGGAR+GC RKL +SKKVKF
Sbjct: 252 QAHVELCQIYGTCGSNSICMSSGSYNSTYCVCAPGFSPDPRGGARRGCRRKLNISKKVKF 311

Query: 309 LPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQLDILSNGLW 368
           LPLDFV FRGGV++ISLQ PNIS CEANC+KN+SCVGYTF++DGS+ C LQLD LSNGLW
Sbjct: 312 LPLDFVNFRGGVQQISLQTPNISVCEANCLKNSSCVGYTFTYDGSSQCGLQLDNLSNGLW 371

Query: 369 SPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLIIISIF 428
           SPGMK AAFVKV+NSETDRSNFTGMMY+L+STCP+ I+LRPPP++ DNTTRN+ II++IF
Sbjct: 372 SPGMKIAAFVKVDNSETDRSNFTGMMYKLQSTCPIRISLRPPPDNTDNTTRNIWIIVTIF 431

Query: 429 VAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNSIGRG 488
           +AELISGAVFFCAFLKRF+KYRDMARTLGLESLPAGGPKRFTY ELKTATNDFSNS+G+G
Sbjct: 432 IAELISGAVFFCAFLKRFIKYRDMARTLGLESLPAGGPKRFTYDELKTATNDFSNSVGKG 491

Query: 489 GFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRM 548
           GFGEVFKGELPDKRV+AVKCLKN+ GGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRM
Sbjct: 492 GFGEVFKGELPDKRVIAVKCLKNITGGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRM 551

Query: 549 LVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAYLHEE 608
           LVYEYIPNGSLDKFLF KP PSDS    T+T  E  SLDW +RYRIAIGVARAIAYLHEE
Sbjct: 552 LVYEYIPNGSLDKFLFAKPPPSDS----TDTDQETASLDWGIRYRIAIGVARAIAYLHEE 611

Query: 609 CLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKL 668
           CLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKL
Sbjct: 612 CLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKL 671

Query: 669 GSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVEEKIEEIL 728
           GS+SITTKADVYSFGMVLLEIISGTRNF+TK   VESA WYFP WAFEKAFVEEKIEE+L
Sbjct: 672 GSHSITTKADVYSFGMVLLEIISGTRNFDTKGLAVESAFWYFPSWAFEKAFVEEKIEEVL 731

Query: 729 DRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIPPPDKPSIY 788
           D RIRN+YDSG+HF +VNRMV TAMWCL NQPEMRPPMGKVVKMLEGKLEIP P+KPSIY
Sbjct: 732 DSRIRNQYDSGAHFGIVNRMVQTAMWCLHNQPEMRPPMGKVVKMLEGKLEIPLPEKPSIY 791

Query: 789 FLSEPQ 794
           FLSE Q
Sbjct: 792 FLSEGQ 791

BLAST of MC04g_new0453 vs. ExPASy TrEMBL
Match: A0A6J1JUG9 (Receptor-like serine/threonine-protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111487613 PE=3 SV=1)

HSP 1 Score: 1345 bits (3481), Expect = 0.0
Identity = 653/793 (82.35%), Postives = 710/793 (89.53%), Query Frame = 0

Query: 3   LPLLCLLF-FFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPS 62
           L L  L F FFTSP+ A     P PRNFSAFSISQSPWRP QNL+LLSPNSLFAAGFR  
Sbjct: 5   LALSALFFLFFTSPSPAASQSPPNPRNFSAFSISQSPWRPTQNLLLLSPNSLFAAGFRQL 64

Query: 63  TNNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNN 122
             NSNL++FSVW+ N+STD VVWSANRLSPVNRSA+L IT+SGQLRL++GSGRNLWPSN 
Sbjct: 65  PENSNLFLFSVWYFNISTDAVVWSANRLSPVNRSASLTITASGQLRLDNGSGRNLWPSNA 124

Query: 123 VFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVSNNGKYEFKNSVNLT 182
           V  N NST+L+LR+DGDLIYATWESFQFPTNTILPNQT NETTIVSNNGKY F+ SVNLT
Sbjct: 125 VSANSNSTQLILRNDGDLIYATWESFQFPTNTILPNQTLNETTIVSNNGKYAFEKSVNLT 184

Query: 183 FGTATYWNSGNPFNIFQTDGRIIRNNQGPIFPSDFNSTRLRKLVVDDDGNLKIFSFNSNL 242
           F    YWNSGNPF  F+ +G+I R+NQ PI+P+D+N+TRLRKLVVDDDGNLKIFSFN   
Sbjct: 185 FDKLMYWNSGNPFKDFENNGKINRDNQNPIYPNDYNTTRLRKLVVDDDGNLKIFSFNPIP 244

Query: 243 QRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKLK 302
           +RW VVWQAHVELCQIY TCG NSICMS GSYNSTYCVC PGF PD RGGAR+GC RKL 
Sbjct: 245 RRWDVVWQAHVELCQIYGTCGSNSICMSSGSYNSTYCVCAPGFSPDPRGGARRGCRRKLN 304

Query: 303 VSKKVKFLPLDFVTFRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQLD 362
           +SKKVKFL LDFV FRGGV++ISLQ PNIS CEANC+KN+SCVGYTF++DGS+ C LQLD
Sbjct: 305 ISKKVKFLQLDFVNFRGGVQQISLQTPNISVCEANCLKNSSCVGYTFTYDGSSQCGLQLD 364

Query: 363 ILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNV 422
            LSNGLWSPGMK AAFVKV+NSETDRSNFTGMMY+L+STCP+ I+LRPPP++ DNTTRN+
Sbjct: 365 NLSNGLWSPGMKIAAFVKVDNSETDRSNFTGMMYKLQSTCPIRISLRPPPDNTDNTTRNI 424

Query: 423 LIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDF 482
            II++IF+AELISGAVFFCAFLKRF+KYRDMARTLGLESLPAGGPKRFTY ELKTATNDF
Sbjct: 425 WIIVTIFIAELISGAVFFCAFLKRFIKYRDMARTLGLESLPAGGPKRFTYDELKTATNDF 484

Query: 483 SNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFC 542
           SNS+G+GGFGEVFKGELPDKRV+AVKCLKN+ GGDGDFWSEVTIIARMHHLNLLRLWGFC
Sbjct: 485 SNSVGKGGFGEVFKGELPDKRVIAVKCLKNITGGDGDFWSEVTIIARMHHLNLLRLWGFC 544

Query: 543 AEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARA 602
           AEKGQRMLVYEYIPNGSLDKFLF KP PSDS    T+T  E  SLDW +RYRIAIGVARA
Sbjct: 545 AEKGQRMLVYEYIPNGSLDKFLFTKPPPSDS----TDTDQETASLDWGIRYRIAIGVARA 604

Query: 603 IAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYV 662
           IAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYV
Sbjct: 605 IAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYV 664

Query: 663 APELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVE 722
           APELVKLGS+SITTKADVYSFGMVLLEIISGTRNF+TK   VESA WYFP WAFEKAFVE
Sbjct: 665 APELVKLGSHSITTKADVYSFGMVLLEIISGTRNFDTKGLAVESAFWYFPSWAFEKAFVE 724

Query: 723 EKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLEIPP 782
           EKIEE+LD RIRN+YDSG+HF +VNRMV TAMWCL NQPEMRPPMGKVVKMLEGKLEIP 
Sbjct: 725 EKIEEVLDSRIRNQYDSGAHFGIVNRMVQTAMWCLHNQPEMRPPMGKVVKMLEGKLEIPL 784

Query: 783 PDKPSIYFLSEPQ 794
           P+KPSIYFLSE Q
Sbjct: 785 PEKPSIYFLSEGQ 793

BLAST of MC04g_new0453 vs. TAIR 10
Match: AT1G34300.1 (lectin protein kinase family protein )

HSP 1 Score: 377.5 bits (968), Expect = 2.7e-104
Identity = 286/825 (34.67%), Postives = 396/825 (48.00%), Query Frame = 0

Query: 3   LPLLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPST 62
           LPLL LL  F             P +FS   +    +    N    SPNS F+  F PS 
Sbjct: 11  LPLLLLLLHF-------------PFSFSTIPLGSVIYASGSNQNWPSPNSTFSVSFVPSP 70

Query: 63  NNSNLYVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNNV 122
            + N ++ +V F   +    +WSA     V+   +L + +SG LRL +GSG  +W S   
Sbjct: 71  -SPNSFLAAVSF---AGSVPIWSA---GTVDSRGSLRLHTSGSLRLTNGSGTTVWDSKT- 130

Query: 123 FGNPNSTKLMLRDDGDLI------YATWESFQFPTNTILPNQTFNETTIVSNNGKYEF-- 182
                 T   + D G+ I         W SF  PT+TI+ +Q F    I+  +G Y F  
Sbjct: 131 -DRLGVTSGSIEDTGEFILLNNRSVPVWSSFDNPTDTIVQSQNFTAGKIL-RSGLYSFQL 190

Query: 183 KNSVNLTF---GTATYWNSG----------NPFNIFQTDGRI------IRNNQGPIFPSD 242
           + S NLT     +A YWN G          +P    QT+G +      +      ++  D
Sbjct: 191 ERSGNLTLRWNTSAIYWNHGLNSSFSSNLSSPRLSLQTNGVVSIFESNLLGGAEIVYSGD 250

Query: 243 F-NSTRLRKLVVDDDGNLKIFSFNS-NLQRWGVVWQAHVELCQIYATCGPNSICMSGGSY 302
           + +S   R L +DDDGNL+I+S  S N       W A V+ C +Y  CG   IC    SY
Sbjct: 251 YGDSNTFRFLKLDDDGNLRIYSSASRNSGPVNAHWSA-VDQCLVYGYCGNFGIC----SY 310

Query: 303 NST--YCVCPP-GFIPDSRGGARQGCHRKLKVSK-KVKFLPLDFV-----TFRGGVKEIS 362
           N T   C CP   F        R+GC RK+++S        LD V     T+       S
Sbjct: 311 NDTNPICSCPSRNFDFVDVNDRRKGCKRKVELSDCSGNTTMLDLVHTRLFTYEDDPNSES 370

Query: 363 LQLPNISTCEANCVKNASCVGYTFSFDGSAVC-ALQLDILSNGLWSPGMKKAAFVKVENS 422
                 S C ANC+ +  C+      DGS  C          G   P +   ++VKV   
Sbjct: 371 F-FAGSSPCRANCLSSVLCLASVSMSDGSGNCWQKHPGSFFTGYQWPSVPSTSYVKVCGP 430

Query: 423 ETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLIIISIFVAELISGAV------ 482
                              V  TL    +  DN ++  L I+++ V   + G V      
Sbjct: 431 ------------------VVANTLERATKGDDNNSKVHLWIVAVAVIAGLLGLVAVEIGL 490

Query: 483 FFCAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNSIGRGGFGEVFKGE 542
           ++C   K   ++  ++    L    +G P +FTY EL+  T  F   +G GGFG V++G 
Sbjct: 491 WWCCCRKN-PRFGTLSSHYTLLEYASGAPVQFTYKELQRCTKSFKEKLGAGGFGTVYRGV 550

Query: 543 LPDKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRMLVYEYIPNG 602
           L ++ VVAVK L+ +  G+  F  EV  I+  HHLNL+RL GFC++   R+LVYE++ NG
Sbjct: 551 LTNRTVVAVKQLEGIEQGEKQFRMEVATISSTHHLNLVRLIGFCSQGRHRLLVYEFMRNG 610

Query: 603 SLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAYLHEECLEWVLHRD 662
           SLD FLF              T      L W  R+ IA+G A+ I YLHEEC + ++H D
Sbjct: 611 SLDNFLF--------------TTDSAKFLTWEYRFNIALGTAKGITYLHEECRDCIVHCD 670

Query: 663 IKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKLGSNSITTKA 722
           IKPENIL+D++F  K+SDFGL+KL    +   +MS +RGT GY+APE   L +  IT+K+
Sbjct: 671 IKPENILVDDNFAAKVSDFGLAKLLNPKDNRYNMSSVRGTRGYLAPEW--LANLPITSKS 730

Query: 723 DVYSFGMVLLEIISGTRNFETKRSTVESADWYFPGWAFEKAFVEEKIEEILDRRIRNEYD 782
           DVYS+GMVLLE++SG RNF+    T       F  WA+E+ F +   + ILD R+    D
Sbjct: 731 DVYSYGMVLLELVSGKRNFDVSEKTNHKK---FSIWAYEE-FEKGNTKAILDTRLSE--D 765

BLAST of MC04g_new0453 vs. TAIR 10
Match: AT4G00340.1 (receptor-like protein kinase 4 )

HSP 1 Score: 370.5 bits (950), Expect = 3.3e-102
Identity = 281/839 (33.49%), Postives = 412/839 (49.11%), Query Frame = 0

Query: 3   LPLLCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAGFRPST 62
           LPLL LLF         PPP+            QS    + N  +LS  ++F  GF  +T
Sbjct: 7   LPLLLLLFLL-------PPPS-----------VQSKVIIKGNQTILSFKAIFRLGFFSTT 66

Query: 63  NNSNLYVFSVWFLNVSTDNVVWSANRLSPVN--RSAALAITSSGQL---RLNDG---SGR 122
           N S+ +   + + ++ T   VW ANR+ PV+   S+ L +TS+G L    L DG      
Sbjct: 67  NGSSNWYLGISYASMPTPTHVWVANRIRPVSDPDSSTLELTSTGYLIVSNLRDGVVWQTD 126

Query: 123 NLWPSNNVFGNPNSTKLMLRDDGDLIYATWESFQFPTNTILPNQTFNETTIVS------- 182
           N  P  +   +     +++ DDG  +   W+SF  PT+T LP       T ++       
Sbjct: 127 NKQPGTDFRFSETGNLILINDDGSPV---WQSFDNPTDTWLPGMNVTGLTAMTSWRSLFD 186

Query: 183 -NNGKYEFK-----NSVNLTF-GTATYWNSGN-PFNIF-----QTDGRIIR-NNQGPIFP 242
            + G Y  +     N   L + GT  YW++GN     F      T   I R +   P  P
Sbjct: 187 PSPGFYSLRLSPSFNEFQLVYKGTTPYWSTGNWTGEAFVGVPEMTIPYIYRFHFVNPYTP 246

Query: 243 S-----------DFNSTRLRKLVVDDDGNLKIFSFNSNLQRWGVVWQAHVELCQIYATCG 302
           +             +  RL + +V  +G LK ++++   Q W + W    + C++Y  CG
Sbjct: 247 TASFWYIVPPLDSVSEPRLTRFMVGANGQLKQYTWDPQTQSWNMFWLQPEDPCRVYNLCG 306

Query: 303 PNSICMSGGSYNSTYCVCPPGFIPDSRGGAR-----QGCHRKLKVS--KKVKFLPLDFVT 362
               C    S     C C  GF P +    R      GC R+   S  K   F  +  + 
Sbjct: 307 QLGFC---SSELLKPCACIRGFRPRNDAAWRSDDYSDGCRRENGDSGEKSDTFEAVGDLR 366

Query: 363 FRGGVKEISLQLPNISTCEANCVKNASCVGYTFSFDGSAVCALQLDILSNGLWSPGMKKA 422
           + G VK   LQ+   S+C   C+ N+SCVG+ +  + S +C + L+       SP     
Sbjct: 367 YDGDVKMSRLQVSK-SSCAKTCLGNSSCVGF-YHKEKSNLCKILLE-------SP----- 426

Query: 423 AFVKVENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLIIISIFVAELISG 482
                 N+  + S++TG+     S   ++I        K N +++++I+ S+  +  + G
Sbjct: 427 ------NNLKNSSSWTGV-----SEDVLYIREPKKGNSKGNISKSIIILCSVVGSISVLG 486

Query: 483 AVFF--CAFLKRFVKYRDMARTLGLESLPAGGPKRFTYAELKTATNDFSNSIGRGGFGEV 542
                    LKR  K R   R    +       K F++ EL++ATN FS+ +G GGFG V
Sbjct: 487 FTLLVPLILLKRSRK-RKKTRKQDEDGFAVLNLKVFSFKELQSATNGFSDKVGHGGFGAV 546

Query: 543 FKGELP-DKRVVAVKCLKNVAGGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRMLVYE 602
           FKG LP     VAVK L+    G+ +F +EV  I  + H+NL+RL GFC+E   R+LVY+
Sbjct: 547 FKGTLPGSSTFVAVKRLERPGSGESEFRAEVCTIGNIQHVNLVRLRGFCSENLHRLLVYD 606

Query: 603 YIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAYLHEECLEW 662
           Y+P GSL  +L        S T           L W  R+RIA+G A+ IAYLHE C + 
Sbjct: 607 YMPQGSLSSYL--------SRTSP-------KLLSWETRFRIALGTAKGIAYLHEGCRDC 666

Query: 663 VLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKLGSNS 722
           ++H DIKPENILLD+D+  K+SDFGL+KL   D + V ++ +RGT GYVAPE +      
Sbjct: 667 IIHCDIKPENILLDSDYNAKVSDFGLAKLLGRDFSRV-LATMRGTWGYVAPEWI--SGLP 726

Query: 723 ITTKADVYSFGMVLLEIISGTRNFETKRSTV-----ESADWYFPGWAFEKAFVEEKIEEI 782
           ITTKADVYSFGM LLE+I G RN      T+     E   W+FP WA  +  ++  ++ +
Sbjct: 727 ITTKADVYSFGMTLLELIGGRRNVIVNSDTLGEKETEPEKWFFPPWA-AREIIQGNVDSV 772

Query: 783 LDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKVVKMLEGKLE--IPPPDK 785
           +D R+  EY++      V RM + A+WC+Q+  E+RP MG VVKMLEG +E  +PPP K
Sbjct: 787 VDSRLNGEYNTEE----VTRMATVAIWCIQDNEEIRPAMGTVVKMLEGVVEVTVPPPPK 772

BLAST of MC04g_new0453 vs. TAIR 10
Match: AT2G19130.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 344.7 bits (883), Expect = 1.9e-94
Identity = 248/805 (30.81%), Postives = 395/805 (49.07%), Query Frame = 0

Query: 47  LLSPNSLFAAGFRPSTNNSNLYVFSVWFLNVSTDNVVWSANRLSPV-NRSAALAITSSGQ 106
           ++S +  +  GF    ++SN Y+  +W+  +S   ++W ANR   V ++++++   S+G 
Sbjct: 38  IVSSDGTYEMGFFKPGSSSNFYI-GMWYKQLS-QTILWVANRDKAVSDKNSSVFKISNGN 97

Query: 107 LRLNDGSGRN-LWPSN-NVFGNPNSTKLMLRDDGDLIYAT----------WESFQFPTNT 166
           L L DG+ +  +W +  N   + ++ + +L+DDG+L+  T          W+SF  P +T
Sbjct: 98  LILLDGNYQTPVWSTGLNSTSSVSALEAVLQDDGNLVLRTGGSSLSANVLWQSFDHPGDT 157

Query: 167 ILP-------NQTFNETTIVS-------NNGKYEFKNSVNLTF-----GTATYWNSGNPF 226
            LP        +T     + S       + G +  +   +  +     G+  YW+SG P+
Sbjct: 158 WLPGVKIRLDKRTGKSQRLTSWKSLEDPSPGLFSLELDESTAYKILWNGSNEYWSSG-PW 217

Query: 227 N----IFQT----------DGRIIRNNQGPIFP-SDFNSTRLRKLVVDDDGNLKIFSFNS 286
           N    IF +          +     N     F  S +N   + + V+D  G +K F++  
Sbjct: 218 NPQSRIFDSVPEMRLNYIYNFSFFSNTTDSYFTYSIYNQLNVSRFVMDVSGQIKQFTWLE 277

Query: 287 NLQRWGVVWQAHVELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSR-----GGARQ 346
             + W + W    + CQ+Y  CG   IC      +  +C CP GF P S+          
Sbjct: 278 GNKAWNLFWSQPRQQCQVYRYCGSFGIC---SDKSEPFCRCPQGFRPMSQKDWDLKDYSA 337

Query: 347 GCHRKLKVSKKVKFLPLDFVTFRGGVKEISLQLPN--------------ISTCEANCVKN 406
           GC RK ++              RG + +   +LPN              +S C + C  +
Sbjct: 338 GCVRKTELQ-----------CSRGDINQF-FRLPNMKLADNSEVLTRTSLSICASACQGD 397

Query: 407 ASCVGYTFSFDGSAVCALQLDILSNGLWSPGMKKAAFVKVENSETDRSNFTGMMYRLEST 466
            SC  Y +  +GS+ C          +WS  +     ++ ENSE +         RL ++
Sbjct: 398 CSCKAYAYD-EGSSKCL---------VWSKDVLNLQQLEDENSEGNI-----FYLRLAAS 457

Query: 467 CPVHITLRPPPEDKDNTTRNVLIIISIFVAELISGAVFFCAFLKRFVKYRDMARTLGLES 526
              ++       +K      VL  + + V  L+         +   ++YR   R  G + 
Sbjct: 458 DVPNVGASGKSNNKGLIFGAVLGSLGVIVLVLL--------VVILILRYRRRKRMRGEKG 517

Query: 527 LPAGGPKRFTYAELKTATNDFSNSIGRGGFGEVFKGELPDKRVVAVKCLKNVAGGDGDFW 586
              G    F+Y EL+ AT +FS+ +G GGFG VFKG LPD   +AVK L+ ++ G+  F 
Sbjct: 518 --DGTLSAFSYRELQNATKNFSDKLGGGGFGSVFKGALPDSSDIAVKRLEGISQGEKQFR 577

Query: 587 SEVTIIARMHHLNLLRLWGFCAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETA 646
           +EV  I  + H+NL+RL GFC+E  +++LVY+Y+PNGSLD  LF+               
Sbjct: 578 TEVVTIGTIQHVNLVRLRGFCSEGSKKLLVYDYMPNGSLDSHLFL------------NQV 637

Query: 647 GENPSLDWSVRYRIAIGVARAIAYLHEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSK 706
            E   L W +R++IA+G AR +AYLH+EC + ++H DIKPENILLD+ FCPK++DFGL+K
Sbjct: 638 EEKIVLGWKLRFQIALGTARGLAYLHDECRDCIIHCDIKPENILLDSQFCPKVADFGLAK 697

Query: 707 LRKNDETAVSMSRIRGTPGYVAPELVKLGSNSITTKADVYSFGMVLLEIISGTRNFETKR 766
           L   D + V ++ +RGT GY+APE +     +IT KADVYS+GM+L E++SG RN  T++
Sbjct: 698 LVGRDFSRV-LTTMRGTRGYLAPEWI--SGVAITAKADVYSYGMMLFELVSGRRN--TEQ 757

Query: 767 STVESADWYFPGWAFEKAFVEEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQP 786
           S  E    +FP WA      +  I  ++D R+  +         V R    A WC+Q++ 
Sbjct: 758 SENEKVR-FFPSWAATILTKDGDIRSLVDPRLEGD---AVDIEEVTRACKVACWCIQDEE 778

BLAST of MC04g_new0453 vs. TAIR 10
Match: AT4G32300.1 (S-domain-2 5 )

HSP 1 Score: 341.3 bits (874), Expect = 2.1e-93
Identity = 255/814 (31.33%), Postives = 404/814 (49.63%), Query Frame = 0

Query: 32  FSISQSPWRPEQNLILLSPNSLFAAGFRPSTNNSNLYVFSVWFLNVSTDNVVWSANRLSP 91
           F  SQ  +     + L S NS F  GF  + ++  L+  S+  ++ S+  ++WSANR SP
Sbjct: 33  FGGSQMNYINNDGIFLESNNSAFGFGFVTTQDSVTLFTLSI--IHKSSTKLIWSANRASP 92

Query: 92  VNRSAALAITSSGQLRLNDGSGRNLWPSNNVFGNPNSTKLMLRDDGDLIYAT------WE 151
           V+ S       +G + +    G  +W  +N     N++++ LRD G+L+  +      WE
Sbjct: 93  VSNSDKFVFDDNGNVVM---EGTEVWRLDN--SGKNASRIELRDSGNLVVVSVDGTSIWE 152

Query: 152 SFQFPTNTILPNQTFNE-----TTIVSNNGKY--EFKN-----SVNLTFGTATYWNSGNP 211
           SF  PT+T++ NQ F E     ++  S+N  Y  E K+     SVN +     YW+  N 
Sbjct: 153 SFDHPTDTLITNQAFKEGMKLTSSPSSSNMTYALEIKSGDMVLSVN-SLTPQVYWSMANA 212

Query: 212 -FNIFQTDGRIIRN-----NQGPIFPSDFNSTRLRKLVVDDD-----------GNLKIFS 271
              I   DG ++ +     N    F  D     L + V  D+           GN  + S
Sbjct: 213 RERIINKDGGVVTSSSLLGNSWRFF--DQKQVLLWQFVFSDNKDDNTTWIAVLGNNGVIS 272

Query: 272 FNSNLQRWGVVWQAHV----ELCQIYATCGPNSICMSGGSYNSTYCVCPPGFIPDSRGGA 331
           F SNL        +      +LC     CGP  +C       S  C C  G +  +R   
Sbjct: 273 F-SNLGSGASAADSSTKIPSDLCGTPEPCGPYYVCS-----GSKVCGCVSG-LSRARSDC 332

Query: 332 RQGCHRKLKVSKKVKFLPLDFVTFRGGVKEISL-------QLPNISTCEANCVKNASCVG 391
           + G     K +K    LPL  V+   GV   +L       +  ++ +C+  C  N SC+G
Sbjct: 333 KTGITSPCKKTKDNATLPLQLVSAGDGVDYFALGYAPPFSKKTDLDSCKEFCHNNCSCLG 392

Query: 392 YTFSFDGSAVCALQLDILSNGLWS--PGMKKAAFVKV--------ENSETDRSNFTGMMY 451
             F  + S  C L  D + +   S   G    +++K+        +N E D  +F  ++ 
Sbjct: 393 LFFQ-NSSGNCFL-FDYIGSFKTSGNGGSGFVSYIKIASTGSGGGDNGEDDGKHFPYVV- 452

Query: 452 RLESTCPVHITLRPPPEDKDNTTRNVLIIISIFVAELISGAVFFCAFLKRFV---KYRDM 511
                                    +++++++F+  ++    F     K+ +        
Sbjct: 453 -------------------------IIVVVTVFIIAVLIFVAFRIHKRKKMILEAPQESS 512

Query: 512 ARTLGLESLPAGGPKRFTYAELKTATNDFSNSIGRGGFGEVFKGELPDKRVVAVKCLKNV 571
                LE+L +G P RF Y +L++ATN+FS  +G+GGFG V++G LPD   +AVK L+ +
Sbjct: 513 EEDNFLENL-SGMPIRFAYKDLQSATNNFSVKLGQGGFGSVYEGTLPDGSRLAVKKLEGI 572

Query: 572 AGGDGDFWSEVTIIARMHHLNLLRLWGFCAEKGQRMLVYEYIPNGSLDKFLFIKPQPSDS 631
             G  +F +EV+II  +HHL+L+RL GFCAE   R+L YE++  GSL++++F K    D 
Sbjct: 573 GQGKKEFRAEVSIIGSIHHLHLVRLRGFCAEGAHRLLAYEFLSKGSLERWIFRK---KDG 632

Query: 632 ETDATETAGENPSLDWSVRYRIAIGVARAIAYLHEECLEWVLHRDIKPENILLDNDFCPK 691
           +            LDW  R+ IA+G A+ +AYLHE+C   ++H DIKPENILLD++F  K
Sbjct: 633 DV----------LLDWDTRFNIALGTAKGLAYLHEDCDARIVHCDIKPENILLDDNFNAK 692

Query: 692 LSDFGLSKLRKNDETAVSMSRIRGTPGYVAPELVKLGSNSITTKADVYSFGMVLLEIISG 751
           +SDFGL+KL   +++ V  + +RGT GY+APE +   + +I+ K+DVYS+GMVLLE+I G
Sbjct: 693 VSDFGLAKLMTREQSHV-FTTMRGTRGYLAPEWIT--NYAISEKSDVYSYGMVLLELIGG 752

Query: 752 TRNFETKRSTVESADWYFPGWAFEKAFVEEKIEEILDRRIRNEYDSGSHFAMVNRMVSTA 787
            +N++   +   S   +FP +AF+K   E K+ +I+D +++N   +      V R + TA
Sbjct: 753 RKNYDPSET---SEKCHFPSFAFKK-MEEGKLMDIVDGKMKNVDVTDER---VQRAMKTA 777

BLAST of MC04g_new0453 vs. TAIR 10
Match: AT5G35370.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 296.6 bits (758), Expect = 6.0e-80
Identity = 248/861 (28.80%), Postives = 397/861 (46.11%), Query Frame = 0

Query: 6   LCLLFFFTSPAAAQPPPAPKPRNFSAFSISQSPWRPEQNLILLSPNSLFAAG-FRPSTNN 65
           L LLF F S A++     P   NF+A ++        +   LLS NS+F AG F P  ++
Sbjct: 14  LNLLFVFVSCASSIEFVYP---NFTASNLRFVD--SSKGAFLLSRNSIFKAGLFSPGGDD 73

Query: 66  SNL-YVFSVWFLNVSTDNVVWSANRLSPVNRSAALAITSSGQLRLNDGSGRNLWPSNNVF 125
           S+  + FSV  ++V + + +WS+NR SPV+ S  + +T  G   + DG  +    S  V 
Sbjct: 74  SSTGFYFSV--VHVDSGSTIWSSNRDSPVSSSGTMNLTPQGISVIEDGKSQIPVWSTPVL 133

Query: 126 GNPNSTKLMLRDDGDLI------YATWESFQFPTNTILPNQTFNETTIVS--------NN 185
            +P  + L L D G+L+       + WESF FPT++I+  Q       +S        + 
Sbjct: 134 ASPVKS-LRLTDAGNLLLLDHLNVSLWESFDFPTDSIVLGQRLKLGMFLSGSVSRSDFST 193

Query: 186 GKYEF---KNSVNLTFGTATYW--------NSGNPFNI----FQTDGRIIRNNQGPIF-- 245
           G Y+F   ++   + +    YW        N  + F +      T G  +    G +   
Sbjct: 194 GDYKFLVGESDGLMQWRGQNYWKLRMHIRANVDSNFPVEYLTVTTSGLALMARNGTVVVV 253

Query: 246 ------PSDFNSTRLRKLVVDDDGNLKIFSFNSNLQRWGVVWQAHVELCQIYATCGPNSI 305
                  SDF   ++     D  G   +  F+   +     +   ++ CQI   CG   +
Sbjct: 254 RVALPPSSDFRVAKM-----DSSGKFIVSRFSG--KNLVTEFSGPMDSCQIPFVCGKLGL 313

Query: 306 CMSGGSYNSTYCVCPPGFIPDSRGGARQGCHRKLKVSKKVKFLPLDFVTFRGGVKEISLQ 365
           C    +  +  C CP     D+  G      + L +    +   + ++    GV   S  
Sbjct: 314 CNLDNASENQSCSCPDEMRMDAGKGVCVPVSQSLSLPVSCEARNISYLELGLGVSYFSTH 373

Query: 366 LPN-------ISTCEANCVKNASCVGYTFSFDGSAVCALQLDILSNGLWSPGMKKAAFVK 425
             +       +  C   C KN SC+G  F  + S  C L  D   +          + VK
Sbjct: 374 FTDPVEHGLPLLACHDICSKNCSCLG-VFYENTSRSCYLVKDSFGS---------LSLVK 433

Query: 426 VENSETDRSNFTGMMYRLESTCPVHITLRPPPEDKDNTTRNVLIII----SIFVAELISG 485
                 D   +  +  R  +  P      P   ++  ++  V+ ++    S F   +  G
Sbjct: 434 NSPENHDLIGYVKLSIRKTNAQP------PGNNNRGGSSFPVIALVLLPCSGFFLLIALG 493

Query: 486 AVFF--CAFLK-RFVKYRDMARTLGLESLPAGG------PKRFTYAELKTATNDFSNSIG 545
            +++  CA ++   ++ + + R    ES   G       P++F + EL+ AT +F   IG
Sbjct: 494 LLWWRRCAVMRYSSIREKQVTRPGSFESGDLGSFHIPGLPQKFEFEELEQATENFKMQIG 553

Query: 546 RGGFGEVFKGELPDKRVVAVKCLKNVA-GGDGDFWSEVTIIARMHHLNLLRLWGFCAEKG 605
            GGFG V+KG LPD+ ++AVK + N    G  +F +E+ II  + H NL++L GFCA   
Sbjct: 554 SGGFGSVYKGTLPDETLIAVKKITNHGLHGRQEFCTEIAIIGNIRHTNLVKLRGFCARGR 613

Query: 606 QRMLVYEYIPNGSLDKFLFIKPQPSDSETDATETAGENPSLDWSVRYRIAIGVARAIAYL 665
           Q +LVYEY+ +GSL+K LF               +G  P L+W  R+ IA+G AR +AYL
Sbjct: 614 QLLLVYEYMNHGSLEKTLF---------------SGNGPVLEWQERFDIALGTARGLAYL 673

Query: 666 HEECLEWVLHRDIKPENILLDNDFCPKLSDFGLSKLRKNDETAVSMSRIRGTPGYVAPEL 725
           H  C + ++H D+KPENILL + F PK+SDFGLSKL   +E+++  + +RGT GY+APE 
Sbjct: 674 HSGCDQKIIHCDVKPENILLHDHFQPKISDFGLSKLLNQEESSL-FTTMRGTRGYLAPEW 733

Query: 726 VKLGSNSITTKADVYSFGMVLLEIISGTRNF----------------ETKRSTVESADWY 785
           +   + +I+ KADVYS+GMVLLE++SG +N                  +  +T  +   Y
Sbjct: 734 IT--NAAISEKADVYSYGMVLLELVSGRKNCSFRSRSNSVTEDNNQNHSSTTTTSTGLVY 793

Query: 786 FPGWAFEKAFVEEKIEEILDRRIRNEYDSGSHFAMVNRMVSTAMWCLQNQPEMRPPMGKV 791
           FP +A +    + +  E+ D R+     S        ++V  A+ C+  +P +RP M  V
Sbjct: 794 FPLYALD-MHEQGRYMELADPRLEGRVTSQE----AEKLVRIALCCVHEEPALRPTMAAV 820

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XID33.8e-10334.67G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 OS=Arabi... [more]
Q392032.8e-9832.90G-type lectin S-receptor-like serine/threonine-protein kinase SD2-2 OS=Arabidops... [more]
Q9FLV41.4e-9729.60G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabi... [more]
P178015.5e-9432.04Putative receptor protein kinase ZmPK1 OS=Zea mays OX=4577 GN=PK1 PE=2 SV=2[more]
O644772.7e-9330.81G-type lectin S-receptor-like serine/threonine-protein kinase At2g19130 OS=Arabi... [more]
Match NameE-valueIdentityDescription
XP_022146471.10.099.12G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 [Momordi... [more]
XP_038875239.10.084.49G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 [Beninca... [more]
XP_038906503.10.083.59G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 [Beninca... [more]
XP_008467083.10.083.50PREDICTED: G-type lectin S-receptor-like serine/threonine-protein kinase At1g343... [more]
KAA0046397.10.083.38G-type lectin S-receptor-like serine/threonine-protein kinase [Cucumis melo var.... [more]
Match NameE-valueIdentityDescription
A0A6J1CZG10.099.12Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=... [more]
A0A1S3CSP70.083.50Receptor-like serine/threonine-protein kinase OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A5A7TWW00.083.38Receptor-like serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1HG240.083.21Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JUG90.082.35Receptor-like serine/threonine-protein kinase OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT1G34300.12.7e-10434.67lectin protein kinase family protein [more]
AT4G00340.13.3e-10233.49receptor-like protein kinase 4 [more]
AT2G19130.11.9e-9430.81S-locus lectin protein kinase family protein [more]
AT4G32300.12.1e-9331.33S-domain-2 5 [more]
AT5G35370.16.0e-8028.80S-locus lectin protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 479..773
e-value: 1.9E-28
score: 110.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 483..766
e-value: 4.6E-45
score: 154.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 479..777
score: 38.802788
IPR001480Bulb-type lectin domainSMARTSM00108blect_4coord: 43..150
e-value: 1.8E-4
score: 30.3
IPR001480Bulb-type lectin domainPFAMPF01453B_lectincoord: 82..162
e-value: 2.7E-12
score: 47.1
IPR001480Bulb-type lectin domainPROSITEPS50927BULB_LECTINcoord: 32..154
score: 12.888235
IPR001480Bulb-type lectin domainCDDcd00028B_lectincoord: 47..150
e-value: 2.24863E-19
score: 82.3599
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 449..553
e-value: 2.6E-30
score: 106.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 554..779
e-value: 7.9E-56
score: 190.6
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 480..691
e-value: 1.4E-13
score: 47.6
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 483..688
e-value: 2.6E-9
score: 33.0
NoneNo IPR availablePANTHERPTHR47974:SF6OS10G0101000 PROTEINcoord: 9..792
NoneNo IPR availablePANTHERPTHR47974OS07G0415500 PROTEINcoord: 9..792
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 485..775
e-value: 8.05322E-91
score: 284.55
IPR024171S-receptor-like serine/threonine-protein kinasePIRSFPIRSF000641SRKcoord: 1..795
e-value: 6.3E-189
score: 627.1
IPR036426Bulb-type lectin domain superfamilyGENE3D2.90.10.10coord: 36..154
e-value: 9.9E-11
score: 43.7
IPR036426Bulb-type lectin domain superfamilySUPERFAMILY51110alpha-D-mannose-specific plant lectinscoord: 68..192
IPR000858S-locus glycoprotein domainPFAMPF00954S_locus_glycopcoord: 217..287
e-value: 4.7E-12
score: 46.4
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 613..625
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 485..507
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 251..290
score: 8.877701
IPR003609PAN/Apple domainPROSITEPS50948PANcoord: 296..384
score: 7.317037
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 461..774

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g_new0453.1MC04g_new0453.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0048544 recognition of pollen
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity