MC04g2021 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g2021 vs. ExPASy Swiss-Prot
Match: I3XHK1 (DEAD-box ATP-dependent RNA helicase FANCM OS=Arabidopsis thaliana OX=3702 GN=FANCM PE=2 SV=1)

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 689/1380 (49.93%), Postives = 868/1380 (62.90%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            +FDWEAAVKEID+ACL    ++SS S H + +A       NP   P      K  A RQ 
Sbjct: 15   EFDWEAAVKEIDLACLKTTNASSSSSSHFTPLA-------NP---PITANLTKPPAKRQS 74

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVN--VPLRDY 120
            TL +F+     K  N  +  E  V D     +   L+ ID EAAKTWIYPVN  VPLRDY
Sbjct: 75   TLDKFIGRTEHKPENHQVVSECGVND----NDNSPLVGIDPEAAKTWIYPVNGSVPLRDY 134

Query: 121  QLAITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEAC 180
            Q AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFP+GKIVF APSRPLV+QQIEAC
Sbjct: 135  QFAITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPQGKIVFAAPSRPLVMQQIEAC 194

Query: 181  HNIVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVID 240
            HNIVGIPQEWTID+TG   P+KRA  WKSKRVFFVTPQVLEKDIQSGTCL  YLVCLVID
Sbjct: 195  HNIVGIPQEWTIDLTGQTCPSKRAFLWKSKRVFFVTPQVLEKDIQSGTCLTNYLVCLVID 254

Query: 241  EAHRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESD 300
            EAHRALGNYSYCV VRELM VP+ LRILALTATPGSK QAIQ IIDNL ISTLEYR+ESD
Sbjct: 255  EAHRALGNYSYCVVVRELMAVPIQLRILALTATPGSKTQAIQGIIDNLQISTLEYRNESD 314

Query: 301  HDVSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDL 360
            HDV PYVHDRK+E+++V +G+DA +++ +L  VIRP+  +L + G+  NRD +TLSP ++
Sbjct: 315  HDVCPYVHDRKLEVIEVPLGQDADDVSKRLFHVIRPYAVRLKNFGVNLNRDIQTLSPHEV 374

Query: 361  LNSRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGS 420
            L +RDKFRQAP P  PH+   DVE  F  LITLYHIRKLLSSHGIRPA EMLEEKL +G 
Sbjct: 375  LMARDKFRQAPLPGLPHVNHGDVESCFAALITLYHIRKLLSSHGIRPAYEMLEEKLKEGP 434

Query: 421  FARFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRG 480
            FAR MSKN+ I   +L+M++ LSHGAPSPKLSKM+E+LVDHFK KDP+ SRVIIFSNFRG
Sbjct: 435  FARLMSKNEDIRMTKLLMQQRLSHGAPSPKLSKMLEILVDHFKVKDPKTSRVIIFSNFRG 494

Query: 481  SVRDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGE 540
            SVRDIM+AL+ IGD+V+ATEFIGQSSGKTLKGQSQK+QQAVLEKFRAGG+NVIVATSIGE
Sbjct: 495  SVRDIMNALSNIGDMVKATEFIGQSSGKTLKGQSQKIQQAVLEKFRAGGFNVIVATSIGE 554

Query: 541  EGLDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKS 600
            EGLDIMEVDLVICFDAN+SP+RMIQRMGRTGRK++GRV +L+CEGSE   YMRKQ + ++
Sbjct: 555  EGLDIMEVDLVICFDANVSPLRMIQRMGRTGRKNNGRVVVLACEGSEKNSYMRKQASGRA 614

Query: 601  IKRHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKK 660
            IK+HM+NGG NSFNFH  PRMI H  +PEVQ V+ SIKQFV  GKK+++++  +T  F+K
Sbjct: 615  IKKHMRNGGTNSFNFHPSPRMIPHVYKPEVQHVEFSIKQFVPRGKKLQEEYATETPAFQK 674

Query: 661  KLTDVETKLLLKYF-HPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEG 720
            KLT  ET +L KY+ +P E+  R SLIAFPHFQT PS+ H VMHS +T +LID MQHL+ 
Sbjct: 675  KLTPAETHMLAKYYNNPDEEKLRVSLIAFPHFQTLPSKVHKVMHSRQTGMLIDAMQHLQE 734

Query: 721  LHFSRDSEAISVSLNEVNASKAELDGPL--------VH--------------PEVLPPPN 780
              FS  S++           + ELD  L        +H               +V  P +
Sbjct: 735  PTFSEQSKSFFTEFRAPLGEREELDTGLRVTNDPKDLHSVRDLEVNTSQRKAKQVESPTS 794

Query: 781  P--------AENNCISDYCFSKHSINIDHFGSKI-----------LLSSELGSCSVRTAQ 840
                      E++    Y FS    ++D  G+             +L S+         Q
Sbjct: 795  TLETTEKDYEESSPTHRYLFSSECASVDTLGNVFVMPVPLLFFPNVLESDNTPLPKTEKQ 854

Query: 841  DLTVQTEAVN-KPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIHQTAISNRTLFN 900
                 T  ++  P  +  K  QD I    +        D A   +ET H     N T   
Sbjct: 855  HSCRNTSHIDLVPVDTSEKHRQDNISCKLKE---RFSPDGASETLET-HSLVKRNSTRVG 914

Query: 901  KGDSASEM-PVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPIDDCGNSSRQR 960
            + D A+ +  +VL  +      D+C  +E  LSPRLTN +KSG VP+SP+ D G ++R+ 
Sbjct: 915  EDDVANSVGEIVLSSD-----EDDCEGLE--LSPRLTNFIKSGIVPESPVYDQGEANREE 974

Query: 961  IYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGEVQPSLLKEND 1020
              EF  P+   P++   E   +S       +  CN  +       L T + +  L    +
Sbjct: 975  DLEF--PQLSSPMRFSNELAGESSFPERKVQHKCNDYNIVSTTTELRTPQKEVGLANGTE 1034

Query: 1021 SVIIKSRASISPVADETQTPLAII--ASSCGSEDWHLVPGEKSSSIQKPRKFKRLRKIGD 1080
             +      ++SP+ ++ +TPLA +   +S   +DW +  GEK  ++++PRK KRLR++GD
Sbjct: 1035 CL------AVSPIPEDWRTPLANLTNTNSSARKDWRVSSGEKLETLRQPRKLKRLRRLGD 1094

Query: 1081 VEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAFIEEEAEVTSDA 1140
                    S V    P +       S +R  K  +   +  +DD+V+ FI+EEAEV+S A
Sbjct: 1095 ------CSSAVKENYPGITEADHIRSRSRGKKHIRGKKKMIMDDDVQVFIDEEAEVSSGA 1154

Query: 1141 TISEDEEDDKIKSSF-DSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLLSQSPF----- 1200
             +S DE +D    SF DSFIDD    +    TQ E+G+ DMMAVYRRSLLSQSP      
Sbjct: 1155 EMSADENEDVTGDSFEDSFIDDGTMPTAN--TQAESGKVDMMAVYRRSLLSQSPLPARFR 1214

Query: 1201 ---GRLTSPLSA--RVAETESENSSGKTLNIFHSTLKEHVNQSSEYVKMNRSPEVVTSAT 1260
                   SP SA       ES + S K+L+    T K   ++S++   M  +  VV  ++
Sbjct: 1215 DLAASSLSPYSAGPLTRINESRSDSDKSLSSLR-TPKTTNSESNQDAMMIGNLSVVQISS 1274

Query: 1261 GCCSRAAEVESRKKILTFYASESVPKLSLEKLF--EFEVTGRESMNDVDLNGNVL----N 1308
                     +SRK+  +   S + P ++LE  F    + T +ES   V  N   L    +
Sbjct: 1275 ---------DSRKRKFSLCNSANAPVINLESKFAAHAQATEKESHEGVRSNAGALEYNDD 1334

BLAST of MC04g2021 vs. ExPASy Swiss-Prot
Match: Q8BGE5 (Fanconi anemia group M protein homolog OS=Mus musculus OX=10090 GN=Fancm PE=1 SV=3)

HSP 1 Score: 456.1 bits (1172), Expect = 1.4e-126
Identity = 244/574 (42.51%), Postives = 346/574 (60.28%), Query Frame = 0

Query: 103 AAKTWIYPVNVPLRDYQLAITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIV 162
           A   WIYP N P+RDYQL I+++ALF NTLV LPTGLGKT IAAVVMYN++RWFP GK+V
Sbjct: 67  AGALWIYPTNCPVRDYQLDISRSALFCNTLVCLPTGLGKTFIAAVVMYNFYRWFPSGKVV 126

Query: 163 FTAPSRPLVLQQIEACHNIVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDI 222
           F AP++PLV QQ+EAC +++GIPQ    +MTG      R   W S+RV F+TPQV+  D+
Sbjct: 127 FMAPTKPLVTQQMEACFHVMGIPQSHMAEMTGSTQAVNRKEIWSSRRVLFLTPQVMVNDL 186

Query: 223 QSGTCLVKYLVCLVIDEAHRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQI 282
             G     ++ CLV+DEAH+ALGNY+YC  VREL+      RILAL+ATPGS  +A+QQ+
Sbjct: 187 TRGAVPATHVKCLVVDEAHKALGNYAYCQVVRELVKYTTHFRILALSATPGSDIKAVQQV 246

Query: 283 IDNLHISTLEYRDESDHDVSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSM 342
           I NL I  +E R E   D+ PY H+R++E + V +GE+   I    ++++  F + L   
Sbjct: 247 ITNLLIGKIELRSEESPDILPYSHERRVEKLVVPLGEELGAIQKTYIQILETFASSLIHR 306

Query: 343 GMLQNRDYRTLSPCDLLNSRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHG 402
            +L  RD   L+   ++ +RD+FR+ P P    I+   +EG F + I+LYH  +LL   G
Sbjct: 307 NVLMKRDIPNLTKYQIILARDQFRKNPSPNIVGIQQGIIEGEFALCISLYHGYELLQQMG 366

Query: 403 IRP----ASEMLEEKLHQGSFARFMSKNDHICKVRLIMEKSLSHGAPS------------ 462
           +R      S +++           +S+N+   K+   ++ + +  + S            
Sbjct: 367 MRSLYFFLSGIMDGTKGMTRARNELSRNEDFMKLYTHLQSAFAPASTSDASAFQRGNKEK 426

Query: 463 ------PKLSKMMEVLVDHFKT--------KDPQYSRVIIFSNFRGSVRDIMDALAKIGD 522
                 PKL K+ EV+++HFK+        K    SRV+IFS+FR SV +I + L +   
Sbjct: 427 KFVYSHPKLKKLEEVILEHFKSWNAKATTEKKCHESRVMIFSSFRDSVEEIAEMLLQHRP 486

Query: 523 IVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEGLDIMEVDLVICF 582
           ++R   F+G +SGK  KG +QK Q  V+ +FR GGYN +V+T +GEEGLDI EVDL+ICF
Sbjct: 487 VIRVMTFVGHASGKNTKGFTQKEQLQVVRQFRDGGYNTLVSTCVGEEGLDIGEVDLIICF 546

Query: 583 DANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIKRHMQNGGMNSFN 642
           DA  SPIR+IQRMGRTGRK  GR+ ++  EG E + Y + Q+N K+I + +         
Sbjct: 547 DAQKSPIRLIQRMGRTGRKRQGRIVVILAEGREERTYNQSQSNKKNIYKAISGNRQVLRL 606

Query: 643 FHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVK 647
           + G PRM+   I PE+   KM I   V   +K +
Sbjct: 607 YQGSPRMVPDKINPELH--KMYITHGVYEPEKAR 638

BLAST of MC04g2021 vs. ExPASy Swiss-Prot
Match: Q8IYD8 (Fanconi anemia group M protein OS=Homo sapiens OX=9606 GN=FANCM PE=1 SV=2)

HSP 1 Score: 454.9 bits (1169), Expect = 3.1e-126
Identity = 244/579 (42.14%), Postives = 347/579 (59.93%), Query Frame = 0

Query: 103 AAKTWIYPVNVPLRDYQLAITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIV 162
           A   WIYP N P+RDYQL I++ ALF NTLV LPTGLGKT IAAVVMYN++RWFP GK+V
Sbjct: 79  AGALWIYPTNCPVRDYQLHISRAALFCNTLVCLPTGLGKTFIAAVVMYNFYRWFPSGKVV 138

Query: 163 FTAPSRPLVLQQIEACHNIVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDI 222
           F AP++PLV QQIEAC+ ++GIPQ    +MTG    + R   W SKRV F+TPQV+  D+
Sbjct: 139 FMAPTKPLVTQQIEACYQVMGIPQSHMAEMTGSTQASTRKEIWCSKRVLFLTPQVMVNDL 198

Query: 223 QSGTCLVKYLVCLVIDEAHRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQI 282
             G C    + CLVIDEAH+ALGNY+YC  VREL+      RILAL+ATPGS  +A+QQ+
Sbjct: 199 SRGACPAAEIKCLVIDEAHKALGNYAYCQVVRELVKYTNHFRILALSATPGSDIKAVQQV 258

Query: 283 IDNLHISTLEYRDESDHDVSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSM 342
           I NL I  +E R E   D+  Y H+RK+E + V +GE+   I    ++++  F   L   
Sbjct: 259 ITNLLIGQIELRSEDSPDILTYSHERKVEKLIVPLGEELAAIQKTYIQILESFARSLIQR 318

Query: 343 GMLQNRDYRTLSPCDLLNSRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHG 402
            +L  RD   L+   ++ +RD+FR+ P P    I+   +EG F + I+LYH  +LL   G
Sbjct: 319 NVLMRRDIPNLTKYQIILARDQFRKNPSPNIVGIQQGIIEGEFAICISLYHGYELLQQMG 378

Query: 403 IRP-----------ASEMLEEKLHQGSFARFMSKNDHICKVRLIMEKSLSHGAPS----- 462
           +R               M   K   G    FM   +H+  +      + ++G  +     
Sbjct: 379 MRSLYFFLCGIMDGTKGMTRSKNELGRNEDFMKLYNHLECMFARTRSTSANGISAIQQGD 438

Query: 463 ---------PKLSKMMEVLVDHFKT--------KDPQYSRVIIFSNFRGSVRDIMDALAK 522
                    PKL K+ EV+++HFK+        K    +RV+IFS+FR SV++I + L++
Sbjct: 439 KNKKFVYSHPKLKKLEEVVIEHFKSWNAENTTEKKRDETRVMIFSSFRDSVQEIAEMLSQ 498

Query: 523 IGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEGLDIMEVDLV 582
              I+R   F+G +SGK+ KG +QK Q  V+++FR GGYN +V+T +GEEGLDI EVDL+
Sbjct: 499 HQPIIRVMTFVGHASGKSTKGFTQKEQLEVVKQFRDGGYNTLVSTCVGEEGLDIGEVDLI 558

Query: 583 ICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIKRHMQNGGMN 642
           ICFD+  SPIR++QRMGRTGRK  GR+ I+  EG E + Y + Q+N +SI + + +    
Sbjct: 559 ICFDSQKSPIRLVQRMGRTGRKRQGRIVIILSEGREERIYNQSQSNKRSIYKAISSNRQV 618

Query: 643 SFNFHGCPRMISHAIRPEV--QFVKMSIKQFVRPGKKVK 647
              +   PRM+   I P++   F+   + +  +P + ++
Sbjct: 619 LHFYQRSPRMVPDGINPKLHKMFITHGVYEPEKPSRNLQ 657

BLAST of MC04g2021 vs. ExPASy Swiss-Prot
Match: A0A1D5PRR9 (Fanconi anemia group M protein OS=Gallus gallus OX=9031 GN=FANCM PE=1 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 6.4e-124
Identity = 234/575 (40.70%), Postives = 346/575 (60.17%), Query Frame = 0

Query: 103 AAKTWIYPVNVPLRDYQLAITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIV 162
           A   WIYP N P R YQL + + ALF+NTL+ LPTGLGKT +AAVVMYN++RWFP GK++
Sbjct: 72  AGALWIYPTNRPERPYQLRMARAALFANTLLCLPTGLGKTFVAAVVMYNFYRWFPSGKVL 131

Query: 163 FTAPSRPLVLQQIEACHNIVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDI 222
           F AP++ LV QQ+EAC  ++GIP     +MTG      R   W S+RVFF+TPQ++  D+
Sbjct: 132 FLAPTKALVAQQMEACAQLMGIPGRDMAEMTGGTQALSRRELWASRRVFFLTPQIMVNDL 191

Query: 223 QSGTCLVKYLVCLVIDEAHRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQI 282
             GTC    + CLV+DEAH+ALGN++YC  V+EL       R+LALTATPGS  +A+QQ+
Sbjct: 192 SRGTCPAVEVKCLVVDEAHKALGNHAYCQVVKELSRYTTQFRVLALTATPGSDTKAVQQV 251

Query: 283 IDNLHISTLEYRDESDHDVSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSM 342
           + NL I+ +E   E   ++ PY H+R++E + V +GE+   I    + V+  F  +L  +
Sbjct: 252 VSNLLIAQIELCSEDSPEIQPYSHERQVEKIVVPLGEELGGIQRAYIHVLETFAGRLIKL 311

Query: 343 GMLQNRDYRTLSPCDLLNSRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHG 402
           G+L  RD  +L+   ++ +RD++R+ P P +  ++   +EG F + I+LYH  +LL   G
Sbjct: 312 GVLARRDVPSLTKYQIILARDQYRKNPSPQNVGMQPGIIEGDFALCISLYHGYELLQQMG 371

Query: 403 IRP-----------ASEMLEEKLHQGSFARFM----------------SKNDHICKVRLI 462
           +R            +  +   K   G    FM                S N ++ K R +
Sbjct: 372 VRSLFIYLCGIMDGSKGLTRTKNELGRNEDFMRLYQQLTDMFSDTCQTSANGNLHKSRTV 431

Query: 463 MEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYS------------RVIIFSNFRGSVRDI 522
            E         PKL K+ E++++HFK++    S            RV+IFS+FR SV++I
Sbjct: 432 SENKKEFIYSHPKLKKLEEIVIEHFKSRKMGCSDQTTSGGTCVDTRVMIFSSFRDSVQEI 491

Query: 523 MDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEGLDI 582
            + L++   +VR   F+G S+GK+ KG +QK Q  V+++FR GGYN +V+T +GEEGLDI
Sbjct: 492 AEMLSRFSPVVRVMTFVGHSTGKSTKGFTQKEQLEVVKRFREGGYNTLVSTCVGEEGLDI 551

Query: 583 MEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIKRHM 639
            EVDL+ICFDA  SPIR++QRMGRTGR+  GRV ++  EG E + Y + Q+N +SI++ +
Sbjct: 552 GEVDLIICFDAQKSPIRLVQRMGRTGRQRQGRVVVILAEGREERTYNQSQSNRRSIQKAI 611

BLAST of MC04g2021 vs. ExPASy Swiss-Prot
Match: Q2HG76 (ATP-dependent DNA helicase MPH1 OS=Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) OX=306901 GN=MPH1 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 4.1e-107
Identity = 223/544 (40.99%), Postives = 321/544 (59.01%), Query Frame = 0

Query: 98  DIDEEAAKTWIYPVNV-PLRDYQLAITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWF 157
           ++  +   TW+YP+N+  +RDYQ +I K  LF NTLVALPTGLGKT IAA +M NYFRW 
Sbjct: 169 ELQHDELSTWVYPLNLGAIRDYQFSIVKNGLFHNTLVALPTGLGKTFIAATIMLNYFRWT 228

Query: 158 PEGKIVFTAPSRPLVLQQIEACHNIVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQ 217
              KIVF AP++PL  QQ++AC  I GIP+     +TG   P  R   W+SKR+FF+TPQ
Sbjct: 229 KRSKIVFVAPTKPLASQQVQACLGIAGIPRSQATLLTGETPPVLRQGEWESKRLFFMTPQ 288

Query: 218 VLEKDIQSGTCLVKYLVCLVIDEAHRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQ 277
            L  D+  G    K +V LV+DEAHRA G+Y+Y   V  +      +RILALTATPGS  
Sbjct: 289 TLMNDLSKGYADPKSIVLLVVDEAHRATGDYAYVKVVEFIRRFSKSIRILALTATPGSTV 348

Query: 278 QAIQQIIDNLHISTLEYRDESDHDVSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFV 337
           + +Q IIDNL IS +E R E   D+  YVH R I+ +     ++  E+     + ++P V
Sbjct: 349 EGVQDIIDNLGISHVEIRTEESIDIRQYVHSRNIDTVTFDPSDEMHEVRGLFSKALKPLV 408

Query: 338 AKLCSMGMLQNRDYRTLSPCDLLNSRDKFRQAP-PPAHPHIKCQDVEGFFGVLITLYHIR 397
            KL +  +   RD   L+   LL SR ++   P   A+  +K   +   F +L +L H+ 
Sbjct: 409 DKLSAQNIYYGRDPMNLTTFGLLKSRQEWLAGPGGHANQGVKFM-MMAVFSILQSLAHLI 468

Query: 398 KLLSSHGIRPASEMLEE-------KLHQGS-FARFMSKNDHICKVRLIMEK--SLSHGAP 457
           KLL+ HGI+P    L E       K  QGS   R +  ++   ++  ++E+   +     
Sbjct: 469 KLLNFHGIKPFYNGLAEFRSSEEGKPGQGSKLKRQLLADESFQRMMALIERWMRMEEFNG 528

Query: 458 SPKLSKMMEVLVDHF-KTKDPQYSRVIIFSNFRGSVRDIMDALAKIGDIVRATEFIGQSS 517
            PKL+ + E LV+HF    +   +R I+FS +R S  +I+  L     ++RAT F+GQ+ 
Sbjct: 529 HPKLTYLCETLVNHFIDAGENSNTRAIVFSEYRDSAEEIV-RLLNNQPLIRATVFVGQAD 588

Query: 518 GKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEGLDIMEVDLVICFDANISPIRMIQR 577
            K  +G  QK Q   +EKF+ GG+NV+VATSIGEEGLDI +VDL++C+DA+ SPIRM+QR
Sbjct: 589 SKRSEGMKQKQQIETIEKFKNGGFNVLVATSIGEEGLDIGQVDLIVCYDASASPIRMLQR 648

Query: 578 MGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIKRHMQNGGMNSFNFHGCPRMISHAI 629
           MGRTGRK  G + +L  +G E + ++  + N + +++ + NG   +F      R++   I
Sbjct: 649 MGRTGRKRAGNIVLLLMKGKEEEKFLEAKDNYQKMQQLICNGDGFTFRHDLSTRIVPRDI 708

BLAST of MC04g2021 vs. NCBI nr
Match: XP_022152476.1 (DEAD-box ATP-dependent RNA helicase FANCM [Momordica charantia])

HSP 1 Score: 2495 bits (6466), Expect = 0.0
Identity = 1290/1366 (94.44%), Postives = 1294/1366 (94.73%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP
Sbjct: 27   DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 86

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL
Sbjct: 87   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 146

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN
Sbjct: 147  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 206

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA
Sbjct: 207  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 266

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD
Sbjct: 267  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 326

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRT+    L+ 
Sbjct: 327  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTVWYKTLVA 386

Query: 361  SRDKFRQA-PPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSF 420
                   A PPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSF
Sbjct: 387  LFFLLAPASPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSF 446

Query: 421  ARFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGS 480
            ARFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGS
Sbjct: 447  ARFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGS 506

Query: 481  VRDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEE 540
            VRDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEE
Sbjct: 507  VRDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEE 566

Query: 541  GLDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSI 600
            GLDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRV +L+CEGSELKGYMRKQTNSKSI
Sbjct: 567  GLDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVVVLACEGSELKGYMRKQTNSKSI 626

Query: 601  KRHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKK 660
            KRHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKK
Sbjct: 627  KRHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKK 686

Query: 661  LTDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLH 720
            LTDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLH
Sbjct: 687  LTDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLH 746

Query: 721  FSRDSEAISV------------------------SLNEVNASKAELDGPLVHPEVLPPPN 780
            FSRDSEAISV                        SLNEVNASKAELDGPLVHPEVLPPPN
Sbjct: 747  FSRDSEAISVEEKSCIRELFGAGHIEKCYGGKRGSLNEVNASKAELDGPLVHPEVLPPPN 806

Query: 781  PAENNCISDYCFSKHSINIDHFGS----------------------------------KI 840
            PAENNCISDYCFSKHSINIDHFGS                                  KI
Sbjct: 807  PAENNCISDYCFSKHSINIDHFGSDFVSVDAMGKDQMSHSPVFSTKNFLHPNSVAAGSKI 866

Query: 841  LLSSELGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIE 900
            LLSSELGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIE
Sbjct: 867  LLSSELGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIE 926

Query: 901  TIHQTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPD 960
            TIHQTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPD
Sbjct: 927  TIHQTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPD 986

Query: 961  SPIDDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLS 1020
            SPIDDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLS
Sbjct: 987  SPIDDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLS 1046

Query: 1021 TGEVQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKP 1080
            TGEVQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKP
Sbjct: 1047 TGEVQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKP 1106

Query: 1081 RKFKRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAF 1140
            RKFKRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAF
Sbjct: 1107 RKFKRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAF 1166

Query: 1141 IEEEAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLL 1200
            IEEEAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLL
Sbjct: 1167 IEEEAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLL 1226

Query: 1201 SQSPFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQSSEYVKMNRSPEVVTSAT 1260
            SQSPFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQSSEYVKMNRSPEVVTSAT
Sbjct: 1227 SQSPFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQSSEYVKMNRSPEVVTSAT 1286

Query: 1261 GCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEFY 1307
            GCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEFY
Sbjct: 1287 GCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEFY 1346

BLAST of MC04g2021 vs. NCBI nr
Match: XP_022993248.1 (DEAD-box ATP-dependent RNA helicase FANCM isoform X2 [Cucurbita maxima])

HSP 1 Score: 2046 bits (5302), Expect = 0.0
Identity = 1075/1367 (78.64%), Postives = 1162/1367 (85.00%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            DFDWEAAVKEIDVACLSGI S SSRSLHPSL+AS DASA+NPASFP  EEN+KRG SRQ 
Sbjct: 16   DFDWEAAVKEIDVACLSGIHSASSRSLHPSLLASPDASAENPASFPSPEENEKRGTSRQS 75

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TLHRF+ NPN K + KT +VEEPV+DR +VE+   L+DID+EAAKTWIYPVNVPLRDYQL
Sbjct: 76   TLHRFITNPNAKSQKKTPEVEEPVKDRALVEDSVCLVDIDQEAAKTWIYPVNVPLRDYQL 135

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN
Sbjct: 136  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 195

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA
Sbjct: 196  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 255

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCVA+RELMMV VPLRILALTATPGSKQQ IQQIIDNLHIS L+YRDESDHD
Sbjct: 256  HRALGNYSYCVAIRELMMVRVPLRILALTATPGSKQQGIQQIIDNLHISNLQYRDESDHD 315

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            VSPYVHDRKIEL+QVAMGEDAVEINNKLLE IRPFVAKLCSMG+LQNRDYRTLSPCDLLN
Sbjct: 316  VSPYVHDRKIELIQVAMGEDAVEINNKLLEAIRPFVAKLCSMGILQNRDYRTLSPCDLLN 375

Query: 361  SRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSFA 420
            SRDKFRQAPPP HPHIK QDVEG+FGVLITLYHIRKLLSSHGIRPASEMLEEKL +GSFA
Sbjct: 376  SRDKFRQAPPPVHPHIKYQDVEGYFGVLITLYHIRKLLSSHGIRPASEMLEEKLQRGSFA 435

Query: 421  RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSV 480
            RFMSKNDHICKVRLIMEKSL HGAPSPKLSKMMEVLVDHFKTKDP+ SRVIIFSNFRGSV
Sbjct: 436  RFMSKNDHICKVRLIMEKSLIHGAPSPKLSKMMEVLVDHFKTKDPKDSRVIIFSNFRGSV 495

Query: 481  RDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEG 540
            RDIM ALAKI DIVRATEFIGQSSGK+LKGQSQKVQQAVLEKFRAGGYNVIVAT IGEEG
Sbjct: 496  RDIMGALAKIEDIVRATEFIGQSSGKSLKGQSQKVQQAVLEKFRAGGYNVIVATCIGEEG 555

Query: 541  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIK 600
            LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRV +L+CEGSELKGYMRKQT SKSIK
Sbjct: 556  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVVVLACEGSELKGYMRKQTTSKSIK 615

Query: 601  RHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKKL 660
            +HMQNGG+NSFNFH  PRMI H I+PEVQFVK+SIKQFVRPGKKVKDDH  Q T FK +L
Sbjct: 616  KHMQNGGINSFNFHASPRMIPHTIKPEVQFVKLSIKQFVRPGKKVKDDHAVQITSFKNQL 675

Query: 661  TDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLHF 720
            T+ ET+LLLKYFHPCED W+PSLIAFPHFQTFPSRTH V HSSRTM+LIDTMQHL+GL+F
Sbjct: 676  TNTETELLLKYFHPCEDAWKPSLIAFPHFQTFPSRTHGVRHSSRTMILIDTMQHLQGLYF 735

Query: 721  SRDSEAISV------------------------SLNEVNASKAELDGPLVHPEVLPPPNP 780
            SRDSEA SV                        S+NEVNA KAEL+GP+V+PEV  PPNP
Sbjct: 736  SRDSEAFSVQEKPCIRELFEASQIGKCYGCKRGSINEVNAPKAELEGPIVYPEVSAPPNP 795

Query: 781  AENNCISDYCFSKHSINI-------DHFG------------------------SKILLSS 840
             ENNCIS YC  KH  NI       D  G                        SKILLSS
Sbjct: 796  KENNCISVYCSLKHPSNIGSGFVSVDAMGEDQISRFPVFSSKEILHSNSVDACSKILLSS 855

Query: 841  E-LGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIH 900
              LGS  VRTAQDL V+TEAV++PTTSQTKFLQ+E+L SPETD   VLEDKA+NQIE IH
Sbjct: 856  PGLGSWRVRTAQDLIVRTEAVDEPTTSQTKFLQNEVLPSPETDDATVLEDKAVNQIEKIH 915

Query: 901  QTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPI 960
            Q+ +  RT FN+GD+  E PVV EIEPQFP ADEC+IIETQLSPRLTNL++SGFVPDSPI
Sbjct: 916  QSTVLKRTFFNEGDNTDEKPVVSEIEPQFPPADECNIIETQLSPRLTNLIESGFVPDSPI 975

Query: 961  DDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGE 1020
            D+CG S RQRIYE  I +FIL VQVDGEQLLKS S G ++RINCN GS AG+D+FL++GE
Sbjct: 976  DECGYS-RQRIYESAISQFILHVQVDGEQLLKSSSPGINKRINCNEGSHAGNDIFLASGE 1035

Query: 1021 VQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKPRKF 1080
             +PS+L++NDSV +KS A  SPVA+ETQTP AIIASSC +EDW+LV GEKSSS+QKPRKF
Sbjct: 1036 DRPSVLEDNDSVGVKSHAFTSPVAEETQTPFAIIASSCDNEDWNLVSGEKSSSVQKPRKF 1095

Query: 1081 KRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAFIEE 1140
            KRLRK+GDVEKNEN ES   T V P+AN+VGTFSS+R  KKKKRDGERR +DNVR +IEE
Sbjct: 1096 KRLRKVGDVEKNENTESAEKTSVSPLANMVGTFSSSRHIKKKKRDGERRFEDNVRTYIEE 1155

Query: 1141 EAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLLSQS 1200
            EAEV+SDATIS DEEDDKIKSSFDSFIDDRVSAS  T TQDETG  DMMA+YRRSLLSQS
Sbjct: 1156 EAEVSSDATISGDEEDDKIKSSFDSFIDDRVSASA-TSTQDETGGHDMMAIYRRSLLSQS 1215

Query: 1201 PFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQS----SEYVKMNRSPEVVTSA 1260
            PFGRLTSPL+ RV  TES+ S  KTLN+F +TL  +VNQS    SE+VKM RSPEVV S 
Sbjct: 1216 PFGRLTSPLATRV--TESDTSPDKTLNVFQTTLTGNVNQSHTMHSEHVKMKRSPEVVIST 1275

Query: 1261 TGCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEF 1307
            TG C  A EVESRK+ LTF ASESVPKL+L++ FE  V GRES++DVD         DEF
Sbjct: 1276 TGGCPSATEVESRKRNLTFCASESVPKLNLDREFELVVAGRESISDVD---------DEF 1335

BLAST of MC04g2021 vs. NCBI nr
Match: XP_023551393.1 (DEAD-box ATP-dependent RNA helicase FANCM isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2040 bits (5284), Expect = 0.0
Identity = 1070/1367 (78.27%), Postives = 1160/1367 (84.86%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            DFDWEAAVKEIDVACLSGI S SSRSLH SL+AS DASA+NPASFP  EEN+KRG SRQ 
Sbjct: 16   DFDWEAAVKEIDVACLSGIHSASSRSLHSSLLASPDASAENPASFPSPEENEKRGTSRQS 75

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TLHRF++NPN K + KT +VEEPVQDR +VE+   L+DID+EAAKTWIYPVNVPLRDYQL
Sbjct: 76   TLHRFIINPNAKSQKKTPEVEEPVQDRALVEDSVCLVDIDQEAAKTWIYPVNVPLRDYQL 135

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN
Sbjct: 136  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 195

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA
Sbjct: 196  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 255

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCVA+RELMMVPVPLRILALTATPGSK+Q IQQIIDNLHIS L+YRDESDHD
Sbjct: 256  HRALGNYSYCVAIRELMMVPVPLRILALTATPGSKKQGIQQIIDNLHISNLQYRDESDHD 315

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            VSPYVHDRKIEL+QVAMGEDAVEINNKLLE IRPFVAKLCSMG+LQ+RDYRTLSPCDLLN
Sbjct: 316  VSPYVHDRKIELIQVAMGEDAVEINNKLLEAIRPFVAKLCSMGVLQDRDYRTLSPCDLLN 375

Query: 361  SRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSFA 420
            SRDKFRQAPPP HPHIK QDVEG+FGVLITLYHIRKLLSSHGIRPASEMLEEKL +GSFA
Sbjct: 376  SRDKFRQAPPPVHPHIKYQDVEGYFGVLITLYHIRKLLSSHGIRPASEMLEEKLQRGSFA 435

Query: 421  RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSV 480
            RFMSKNDHICKVRLIMEKSL HGAPSPKLSKMMEVLVDHFKTKDP+ SRVIIFSNFRGSV
Sbjct: 436  RFMSKNDHICKVRLIMEKSLIHGAPSPKLSKMMEVLVDHFKTKDPKDSRVIIFSNFRGSV 495

Query: 481  RDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEG 540
            RDIM ALAKI DIVRATEFIGQSSGK+LKGQSQKVQQAVLEKFRAGGYNVIVAT IGEEG
Sbjct: 496  RDIMGALAKIEDIVRATEFIGQSSGKSLKGQSQKVQQAVLEKFRAGGYNVIVATCIGEEG 555

Query: 541  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIK 600
            LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRV +L+CEGSELKGYMRKQT SKSIK
Sbjct: 556  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVVVLACEGSELKGYMRKQTTSKSIK 615

Query: 601  RHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKKL 660
            +HMQNGG+NSFNFH  PRMI H I+PEVQFVK+SIKQFVRPGKKVKD+H  Q T FK KL
Sbjct: 616  KHMQNGGINSFNFHASPRMIPHTIKPEVQFVKLSIKQFVRPGKKVKDEHAVQITSFKNKL 675

Query: 661  TDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLHF 720
            T+ ET+LLLKYFHPCED W+PSLIAFPHFQTFPSRTH V HSSRTM+LIDTMQHL+GL+F
Sbjct: 676  TNTETELLLKYFHPCEDAWKPSLIAFPHFQTFPSRTHGVRHSSRTMILIDTMQHLQGLYF 735

Query: 721  SRDSEAISV------------------------SLNEVNASKAELDGPLVHPEVLPPPNP 780
            SRDSEA SV                        S+NEV+A K EL+GP+V+PEV  PPNP
Sbjct: 736  SRDSEAFSVQEKPCIRELFEASQIGKCYGGKRGSINEVSAPKTELEGPIVYPEVSAPPNP 795

Query: 781  AENNCISDYCFSKHSINI-------DHFG------------------------SKILLSS 840
             ENNC S YC  KH  NI       D  G                        SKILLSS
Sbjct: 796  TENNCSSVYCSLKHPSNIGSGFVSVDAMGEDQISRFPVFSSKEILHSNSVDACSKILLSS 855

Query: 841  -ELGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIH 900
             ELGS  VRTAQDL V++EAV++PTTSQTKFLQ+E+L SPETD   VLEDKA+NQIE IH
Sbjct: 856  PELGSWRVRTAQDLIVRSEAVDEPTTSQTKFLQNEVLPSPETDDATVLEDKAVNQIEKIH 915

Query: 901  QTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPI 960
            Q+ +  RT FN+GD+  E PVVLEIEPQFP ADECSI+ETQLSPRLTNL++SGFVPDSPI
Sbjct: 916  QSTVLKRTFFNEGDNTDEKPVVLEIEPQFPPADECSIVETQLSPRLTNLIESGFVPDSPI 975

Query: 961  DDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGE 1020
            D+CG S RQRI E  I +FILPVQVDGEQLLKS S G ++RINCN GS AG+DVFL++GE
Sbjct: 976  DECGYS-RQRICESAISQFILPVQVDGEQLLKSSSPGINKRINCNEGSHAGNDVFLASGE 1035

Query: 1021 VQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKPRKF 1080
             +PS+L++NDSV +KS    SPVA+ETQTP AIIASSC +EDW+LV GEKSSS+QKPRKF
Sbjct: 1036 DRPSVLEDNDSVGVKSHGFTSPVAEETQTPFAIIASSCDNEDWNLVSGEKSSSVQKPRKF 1095

Query: 1081 KRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAFIEE 1140
            KRLRK+GDVEKNEN ES   T V P+AN+VGTFSS+R  KKKKRDGERR +DNVRA+IEE
Sbjct: 1096 KRLRKVGDVEKNENTESAEKTSVSPLANMVGTFSSSRHIKKKKRDGERRFEDNVRAYIEE 1155

Query: 1141 EAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLLSQS 1200
            EAEV+SDATIS DEEDDKIKSSFDSFIDDRVSAS  T TQDETG  DMMA+YRRSLLSQS
Sbjct: 1156 EAEVSSDATISGDEEDDKIKSSFDSFIDDRVSASA-TSTQDETGGHDMMAIYRRSLLSQS 1215

Query: 1201 PFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQS----SEYVKMNRSPEVVTSA 1260
            PFGRLTSPL+ RV E  SE S  KTLN+F +TL  ++NQS    SE+VKM RSPEVV S 
Sbjct: 1216 PFGRLTSPLATRVIE--SETSPDKTLNVFQTTLTGNINQSHTMHSEHVKMKRSPEVVIST 1275

Query: 1261 TGCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEF 1307
            TG C  A EVESR + LTF ASESVPKL+L++ FE  V GRES++DVD         DEF
Sbjct: 1276 TGGCPSATEVESRNRNLTFCASESVPKLNLDRQFELVVAGRESISDVD---------DEF 1335

BLAST of MC04g2021 vs. NCBI nr
Match: XP_022939320.1 (DEAD-box ATP-dependent RNA helicase FANCM isoform X2 [Cucurbita moschata])

HSP 1 Score: 2029 bits (5256), Expect = 0.0
Identity = 1067/1367 (78.05%), Postives = 1156/1367 (84.56%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            DFDWEAAVKEIDVACLSGI S SSRSL+ SL+AS  ASA+N ASFP  EEN+KRG SRQ 
Sbjct: 16   DFDWEAAVKEIDVACLSGIHSASSRSLYSSLLASPHASAENSASFPSPEENEKRGTSRQS 75

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TLHRF++NPN K + KT +VEEPVQDR +VE+   L+DID+EAAKTWIYPVNVPLRDYQL
Sbjct: 76   TLHRFIINPNAKSQKKTPEVEEPVQDRALVEDSVCLVDIDQEAAKTWIYPVNVPLRDYQL 135

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN
Sbjct: 136  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 195

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA
Sbjct: 196  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 255

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCVA+RELMMVPVPLRILALTATPGSK+Q IQQIIDNLHIS L+YRDESDHD
Sbjct: 256  HRALGNYSYCVAIRELMMVPVPLRILALTATPGSKKQGIQQIIDNLHISNLQYRDESDHD 315

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            VSPYVHDRKIEL+QVAMGEDA EINNKLLE IRPFVAKLCSMG+LQ+RDYRTLSPCDLLN
Sbjct: 316  VSPYVHDRKIELIQVAMGEDAFEINNKLLEAIRPFVAKLCSMGVLQDRDYRTLSPCDLLN 375

Query: 361  SRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSFA 420
            SRDKFRQAPP  HPHIK QDVEG+FGVLITLYHIRKLLSSHGIRPASEMLEEKL +GSFA
Sbjct: 376  SRDKFRQAPPSVHPHIKYQDVEGYFGVLITLYHIRKLLSSHGIRPASEMLEEKLQRGSFA 435

Query: 421  RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSV 480
            RFMSKNDHICKVRLIMEKSL HGAPSPKLSKMMEVLVDHFKTKDP+ SRVIIFSNFRGSV
Sbjct: 436  RFMSKNDHICKVRLIMEKSLIHGAPSPKLSKMMEVLVDHFKTKDPKDSRVIIFSNFRGSV 495

Query: 481  RDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEG 540
            RDIM ALAKI DIVRATEFIGQSSGK+LKGQSQKVQQAVLEKFRAGGYNVIVAT IGEEG
Sbjct: 496  RDIMGALAKIEDIVRATEFIGQSSGKSLKGQSQKVQQAVLEKFRAGGYNVIVATCIGEEG 555

Query: 541  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIK 600
            LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRV +L+CEGSELKGYMRKQT SKSIK
Sbjct: 556  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVVVLACEGSELKGYMRKQTTSKSIK 615

Query: 601  RHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKKL 660
            +HMQNGG+NSFNFH  PRMI H I+PEVQFVK+SIKQFVRPGKKVKD+H  Q T FK KL
Sbjct: 616  KHMQNGGINSFNFHASPRMIPHTIKPEVQFVKLSIKQFVRPGKKVKDEHAVQITSFKNKL 675

Query: 661  TDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLHF 720
            T+ ET+LLLKYFHPCED W+PS+IAFPHFQTFPSRTH V HSSRTM+LIDTMQHL+GL+F
Sbjct: 676  TNTETELLLKYFHPCEDAWKPSIIAFPHFQTFPSRTHGVRHSSRTMILIDTMQHLQGLYF 735

Query: 721  SRDSEAISV------------------------SLNEVNASKAELDGPLVHPEVLPPPNP 780
            SRDSEA SV                        S+NEV+A K EL+GP+V+PEV  PPNP
Sbjct: 736  SRDSEAFSVQEKPCIRELFEASQIGKCYGGKRGSINEVSAPKTELEGPIVYPEVSAPPNP 795

Query: 781  AENNCISDYCFSKHSINI-------DHFG------------------------SKILLSS 840
             ENNC S YC  KH  NI       D  G                        SKILLSS
Sbjct: 796  TENNCSSVYCSLKHPSNIGSGFVSVDAMGEDQISRFPVFSSKEILHSNSVDACSKILLSS 855

Query: 841  -ELGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIH 900
             ELGS  VRTAQDL V+TEAV++PTTSQTKFLQ+E+L SPETD   VLEDKA+NQIE IH
Sbjct: 856  PELGSWRVRTAQDLIVRTEAVDEPTTSQTKFLQNEVLPSPETDDATVLEDKAVNQIEKIH 915

Query: 901  QTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPI 960
            Q+ +  RT FN+GD+  E PVVLEIEPQFP ADECSIIETQLSPRLTNL++SGFVPDSPI
Sbjct: 916  QSTVLKRTFFNEGDNTDEKPVVLEIEPQFPPADECSIIETQLSPRLTNLIESGFVPDSPI 975

Query: 961  DDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGE 1020
            D+CG S RQRIYE  I +FILPVQVDGEQLLKS S G ++RINCN GS AG+DVFL++GE
Sbjct: 976  DECGYS-RQRIYESAISQFILPVQVDGEQLLKSSSPGINKRINCNEGSHAGNDVFLASGE 1035

Query: 1021 VQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKPRKF 1080
             +PS+L++NDSV +KS    SPVA+ETQTP AIIASSC +EDW+LV GEKSSS+QKPRKF
Sbjct: 1036 DRPSVLEDNDSVGVKSHGFTSPVAEETQTPFAIIASSCDNEDWNLVSGEKSSSVQKPRKF 1095

Query: 1081 KRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAFIEE 1140
            KRLRK+GDVEKNEN ES   T V P+AN+VGTFSS+R  KKKKRDGERR +DNVRA+IEE
Sbjct: 1096 KRLRKVGDVEKNENTESAEKTSVSPLANMVGTFSSSRHIKKKKRDGERRFEDNVRAYIEE 1155

Query: 1141 EAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLLSQS 1200
            EAEV+SDATIS DEEDD IKSSFDSFIDDRVSAS  T TQDETG  DMMA+YRRSLLSQS
Sbjct: 1156 EAEVSSDATISGDEEDDMIKSSFDSFIDDRVSASA-TSTQDETGGHDMMAIYRRSLLSQS 1215

Query: 1201 PFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQS----SEYVKMNRSPEVVTSA 1260
            PFGRLTSP + RV  TESE S  KTLN+F +TL  +VNQS    SE+VKM RSPEVV S 
Sbjct: 1216 PFGRLTSPHATRV--TESETSPDKTLNVFQTTLTGNVNQSHTMHSEHVKMKRSPEVVIST 1275

Query: 1261 TGCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEF 1307
            TG C  A EVESR + LTF ASESVPKL+L++ FE  V GRES++DVD         DEF
Sbjct: 1276 TGGCPSATEVESRNRNLTFCASESVPKLNLDRQFELVVAGRESISDVD---------DEF 1335

BLAST of MC04g2021 vs. NCBI nr
Match: XP_022993247.1 (DEAD-box ATP-dependent RNA helicase FANCM isoform X1 [Cucurbita maxima])

HSP 1 Score: 2024 bits (5245), Expect = 0.0
Identity = 1075/1413 (76.08%), Postives = 1162/1413 (82.24%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            DFDWEAAVKEIDVACLSGI S SSRSLHPSL+AS DASA+NPASFP  EEN+KRG SRQ 
Sbjct: 16   DFDWEAAVKEIDVACLSGIHSASSRSLHPSLLASPDASAENPASFPSPEENEKRGTSRQS 75

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TLHRF+ NPN K + KT +VEEPV+DR +VE+   L+DID+EAAKTWIYPVNVPLRDYQL
Sbjct: 76   TLHRFITNPNAKSQKKTPEVEEPVKDRALVEDSVCLVDIDQEAAKTWIYPVNVPLRDYQL 135

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN
Sbjct: 136  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 195

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA
Sbjct: 196  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 255

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCVA+RELMMV VPLRILALTATPGSKQQ IQQIIDNLHIS L+YRDESDHD
Sbjct: 256  HRALGNYSYCVAIRELMMVRVPLRILALTATPGSKQQGIQQIIDNLHISNLQYRDESDHD 315

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            VSPYVHDRKIEL+QVAMGEDAVEINNKLLE IRPFVAKLCSMG+LQNRDYRTLSPCDLLN
Sbjct: 316  VSPYVHDRKIELIQVAMGEDAVEINNKLLEAIRPFVAKLCSMGILQNRDYRTLSPCDLLN 375

Query: 361  SRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSFA 420
            SRDKFRQAPPP HPHIK QDVEG+FGVLITLYHIRKLLSSHGIRPASEMLEEKL +GSFA
Sbjct: 376  SRDKFRQAPPPVHPHIKYQDVEGYFGVLITLYHIRKLLSSHGIRPASEMLEEKLQRGSFA 435

Query: 421  RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSV 480
            RFMSKNDHICKVRLIMEKSL HGAPSPKLSKMMEVLVDHFKTKDP+ SRVIIFSNFRGSV
Sbjct: 436  RFMSKNDHICKVRLIMEKSLIHGAPSPKLSKMMEVLVDHFKTKDPKDSRVIIFSNFRGSV 495

Query: 481  RDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEG 540
            RDIM ALAKI DIVRATEFIGQSSGK+LKGQSQKVQQAVLEKFRAGGYNVIVAT IGEEG
Sbjct: 496  RDIMGALAKIEDIVRATEFIGQSSGKSLKGQSQKVQQAVLEKFRAGGYNVIVATCIGEEG 555

Query: 541  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIK 600
            LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRV +L+CEGSELKGYMRKQT SKSIK
Sbjct: 556  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVVVLACEGSELKGYMRKQTTSKSIK 615

Query: 601  RHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKKL 660
            +HMQNGG+NSFNFH  PRMI H I+PEVQFVK+SIKQFVRPGKKVKDDH  Q T FK +L
Sbjct: 616  KHMQNGGINSFNFHASPRMIPHTIKPEVQFVKLSIKQFVRPGKKVKDDHAVQITSFKNQL 675

Query: 661  TDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLHF 720
            T+ ET+LLLKYFHPCED W+PSLIAFPHFQTFPSRTH V HSSRTM+LIDTMQHL+GL+F
Sbjct: 676  TNTETELLLKYFHPCEDAWKPSLIAFPHFQTFPSRTHGVRHSSRTMILIDTMQHLQGLYF 735

Query: 721  SRDSEAISV------------------------SLNEVNASKAELDGPLVHPEVLPPPNP 780
            SRDSEA SV                        S+NEVNA KAEL+GP+V+PEV  PPNP
Sbjct: 736  SRDSEAFSVQEKPCIRELFEASQIGKCYGCKRGSINEVNAPKAELEGPIVYPEVSAPPNP 795

Query: 781  AENNCISDYCFSKHSINI-------DHFG------------------------SKILLSS 840
             ENNCIS YC  KH  NI       D  G                        SKILLSS
Sbjct: 796  KENNCISVYCSLKHPSNIGSGFVSVDAMGEDQISRFPVFSSKEILHSNSVDACSKILLSS 855

Query: 841  E-LGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIH 900
              LGS  VRTAQDL V+TEAV++PTTSQTKFLQ+E+L SPETD   VLEDKA+NQIE IH
Sbjct: 856  PGLGSWRVRTAQDLIVRTEAVDEPTTSQTKFLQNEVLPSPETDDATVLEDKAVNQIEKIH 915

Query: 901  QTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPI 960
            Q+ +  RT FN+GD+  E PVV EIEPQFP ADEC+IIETQLSPRLTNL++SGFVPDSPI
Sbjct: 916  QSTVLKRTFFNEGDNTDEKPVVSEIEPQFPPADECNIIETQLSPRLTNLIESGFVPDSPI 975

Query: 961  DDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGE 1020
            D+CG S RQRIYE  I +FIL VQVDGEQLLKS S G ++RINCN GS AG+D+FL++GE
Sbjct: 976  DECGYS-RQRIYESAISQFILHVQVDGEQLLKSSSPGINKRINCNEGSHAGNDIFLASGE 1035

Query: 1021 VQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKPRKF 1080
             +PS+L++NDSV +KS A  SPVA+ETQTP AIIASSC +EDW+LV GEKSSS+QKPRKF
Sbjct: 1036 DRPSVLEDNDSVGVKSHAFTSPVAEETQTPFAIIASSCDNEDWNLVSGEKSSSVQKPRKF 1095

Query: 1081 KRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDG-------------- 1140
            KRLRK+GDVEKNEN ES   T V P+AN+VGTFSS+R  KKKKRDG              
Sbjct: 1096 KRLRKVGDVEKNENTESAEKTSVSPLANMVGTFSSSRHIKKKKRDGIFFSHSSIFGISLS 1155

Query: 1141 --------------------------------ERRLDDNVRAFIEEEAEVTSDATISEDE 1200
                                            ERR +DNVR +IEEEAEV+SDATIS DE
Sbjct: 1156 VLNISEDLIEHTFKQSKSDQVATCQYQLLHLGERRFEDNVRTYIEEEAEVSSDATISGDE 1215

Query: 1201 EDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLLSQSPFGRLTSPLSARVA 1260
            EDDKIKSSFDSFIDDRVSAS  T TQDETG  DMMA+YRRSLLSQSPFGRLTSPL+ RV 
Sbjct: 1216 EDDKIKSSFDSFIDDRVSASA-TSTQDETGGHDMMAIYRRSLLSQSPFGRLTSPLATRV- 1275

Query: 1261 ETESENSSGKTLNIFHSTLKEHVNQS----SEYVKMNRSPEVVTSATGCCSRAAEVESRK 1307
             TES+ S  KTLN+F +TL  +VNQS    SE+VKM RSPEVV S TG C  A EVESRK
Sbjct: 1276 -TESDTSPDKTLNVFQTTLTGNVNQSHTMHSEHVKMKRSPEVVISTTGGCPSATEVESRK 1335

BLAST of MC04g2021 vs. ExPASy TrEMBL
Match: A0A6J1DHU7 (DEAD-box ATP-dependent RNA helicase FANCM OS=Momordica charantia OX=3673 GN=LOC111020197 PE=3 SV=1)

HSP 1 Score: 2495 bits (6466), Expect = 0.0
Identity = 1290/1366 (94.44%), Postives = 1294/1366 (94.73%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP
Sbjct: 27   DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 86

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL
Sbjct: 87   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 146

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN
Sbjct: 147  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 206

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA
Sbjct: 207  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 266

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD
Sbjct: 267  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 326

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRT+    L+ 
Sbjct: 327  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTVWYKTLVA 386

Query: 361  SRDKFRQA-PPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSF 420
                   A PPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSF
Sbjct: 387  LFFLLAPASPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSF 446

Query: 421  ARFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGS 480
            ARFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGS
Sbjct: 447  ARFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGS 506

Query: 481  VRDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEE 540
            VRDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEE
Sbjct: 507  VRDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEE 566

Query: 541  GLDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSI 600
            GLDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRV +L+CEGSELKGYMRKQTNSKSI
Sbjct: 567  GLDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVVVLACEGSELKGYMRKQTNSKSI 626

Query: 601  KRHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKK 660
            KRHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKK
Sbjct: 627  KRHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKK 686

Query: 661  LTDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLH 720
            LTDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLH
Sbjct: 687  LTDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLH 746

Query: 721  FSRDSEAISV------------------------SLNEVNASKAELDGPLVHPEVLPPPN 780
            FSRDSEAISV                        SLNEVNASKAELDGPLVHPEVLPPPN
Sbjct: 747  FSRDSEAISVEEKSCIRELFGAGHIEKCYGGKRGSLNEVNASKAELDGPLVHPEVLPPPN 806

Query: 781  PAENNCISDYCFSKHSINIDHFGS----------------------------------KI 840
            PAENNCISDYCFSKHSINIDHFGS                                  KI
Sbjct: 807  PAENNCISDYCFSKHSINIDHFGSDFVSVDAMGKDQMSHSPVFSTKNFLHPNSVAAGSKI 866

Query: 841  LLSSELGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIE 900
            LLSSELGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIE
Sbjct: 867  LLSSELGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIE 926

Query: 901  TIHQTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPD 960
            TIHQTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPD
Sbjct: 927  TIHQTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPD 986

Query: 961  SPIDDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLS 1020
            SPIDDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLS
Sbjct: 987  SPIDDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLS 1046

Query: 1021 TGEVQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKP 1080
            TGEVQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKP
Sbjct: 1047 TGEVQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKP 1106

Query: 1081 RKFKRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAF 1140
            RKFKRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAF
Sbjct: 1107 RKFKRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAF 1166

Query: 1141 IEEEAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLL 1200
            IEEEAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLL
Sbjct: 1167 IEEEAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLL 1226

Query: 1201 SQSPFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQSSEYVKMNRSPEVVTSAT 1260
            SQSPFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQSSEYVKMNRSPEVVTSAT
Sbjct: 1227 SQSPFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQSSEYVKMNRSPEVVTSAT 1286

Query: 1261 GCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEFY 1307
            GCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEFY
Sbjct: 1287 GCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEFY 1346

BLAST of MC04g2021 vs. ExPASy TrEMBL
Match: A0A6J1K1N4 (DEAD-box ATP-dependent RNA helicase FANCM isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489325 PE=3 SV=1)

HSP 1 Score: 2046 bits (5302), Expect = 0.0
Identity = 1075/1367 (78.64%), Postives = 1162/1367 (85.00%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            DFDWEAAVKEIDVACLSGI S SSRSLHPSL+AS DASA+NPASFP  EEN+KRG SRQ 
Sbjct: 16   DFDWEAAVKEIDVACLSGIHSASSRSLHPSLLASPDASAENPASFPSPEENEKRGTSRQS 75

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TLHRF+ NPN K + KT +VEEPV+DR +VE+   L+DID+EAAKTWIYPVNVPLRDYQL
Sbjct: 76   TLHRFITNPNAKSQKKTPEVEEPVKDRALVEDSVCLVDIDQEAAKTWIYPVNVPLRDYQL 135

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN
Sbjct: 136  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 195

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA
Sbjct: 196  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 255

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCVA+RELMMV VPLRILALTATPGSKQQ IQQIIDNLHIS L+YRDESDHD
Sbjct: 256  HRALGNYSYCVAIRELMMVRVPLRILALTATPGSKQQGIQQIIDNLHISNLQYRDESDHD 315

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            VSPYVHDRKIEL+QVAMGEDAVEINNKLLE IRPFVAKLCSMG+LQNRDYRTLSPCDLLN
Sbjct: 316  VSPYVHDRKIELIQVAMGEDAVEINNKLLEAIRPFVAKLCSMGILQNRDYRTLSPCDLLN 375

Query: 361  SRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSFA 420
            SRDKFRQAPPP HPHIK QDVEG+FGVLITLYHIRKLLSSHGIRPASEMLEEKL +GSFA
Sbjct: 376  SRDKFRQAPPPVHPHIKYQDVEGYFGVLITLYHIRKLLSSHGIRPASEMLEEKLQRGSFA 435

Query: 421  RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSV 480
            RFMSKNDHICKVRLIMEKSL HGAPSPKLSKMMEVLVDHFKTKDP+ SRVIIFSNFRGSV
Sbjct: 436  RFMSKNDHICKVRLIMEKSLIHGAPSPKLSKMMEVLVDHFKTKDPKDSRVIIFSNFRGSV 495

Query: 481  RDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEG 540
            RDIM ALAKI DIVRATEFIGQSSGK+LKGQSQKVQQAVLEKFRAGGYNVIVAT IGEEG
Sbjct: 496  RDIMGALAKIEDIVRATEFIGQSSGKSLKGQSQKVQQAVLEKFRAGGYNVIVATCIGEEG 555

Query: 541  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIK 600
            LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRV +L+CEGSELKGYMRKQT SKSIK
Sbjct: 556  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVVVLACEGSELKGYMRKQTTSKSIK 615

Query: 601  RHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKKL 660
            +HMQNGG+NSFNFH  PRMI H I+PEVQFVK+SIKQFVRPGKKVKDDH  Q T FK +L
Sbjct: 616  KHMQNGGINSFNFHASPRMIPHTIKPEVQFVKLSIKQFVRPGKKVKDDHAVQITSFKNQL 675

Query: 661  TDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLHF 720
            T+ ET+LLLKYFHPCED W+PSLIAFPHFQTFPSRTH V HSSRTM+LIDTMQHL+GL+F
Sbjct: 676  TNTETELLLKYFHPCEDAWKPSLIAFPHFQTFPSRTHGVRHSSRTMILIDTMQHLQGLYF 735

Query: 721  SRDSEAISV------------------------SLNEVNASKAELDGPLVHPEVLPPPNP 780
            SRDSEA SV                        S+NEVNA KAEL+GP+V+PEV  PPNP
Sbjct: 736  SRDSEAFSVQEKPCIRELFEASQIGKCYGCKRGSINEVNAPKAELEGPIVYPEVSAPPNP 795

Query: 781  AENNCISDYCFSKHSINI-------DHFG------------------------SKILLSS 840
             ENNCIS YC  KH  NI       D  G                        SKILLSS
Sbjct: 796  KENNCISVYCSLKHPSNIGSGFVSVDAMGEDQISRFPVFSSKEILHSNSVDACSKILLSS 855

Query: 841  E-LGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIH 900
              LGS  VRTAQDL V+TEAV++PTTSQTKFLQ+E+L SPETD   VLEDKA+NQIE IH
Sbjct: 856  PGLGSWRVRTAQDLIVRTEAVDEPTTSQTKFLQNEVLPSPETDDATVLEDKAVNQIEKIH 915

Query: 901  QTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPI 960
            Q+ +  RT FN+GD+  E PVV EIEPQFP ADEC+IIETQLSPRLTNL++SGFVPDSPI
Sbjct: 916  QSTVLKRTFFNEGDNTDEKPVVSEIEPQFPPADECNIIETQLSPRLTNLIESGFVPDSPI 975

Query: 961  DDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGE 1020
            D+CG S RQRIYE  I +FIL VQVDGEQLLKS S G ++RINCN GS AG+D+FL++GE
Sbjct: 976  DECGYS-RQRIYESAISQFILHVQVDGEQLLKSSSPGINKRINCNEGSHAGNDIFLASGE 1035

Query: 1021 VQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKPRKF 1080
             +PS+L++NDSV +KS A  SPVA+ETQTP AIIASSC +EDW+LV GEKSSS+QKPRKF
Sbjct: 1036 DRPSVLEDNDSVGVKSHAFTSPVAEETQTPFAIIASSCDNEDWNLVSGEKSSSVQKPRKF 1095

Query: 1081 KRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAFIEE 1140
            KRLRK+GDVEKNEN ES   T V P+AN+VGTFSS+R  KKKKRDGERR +DNVR +IEE
Sbjct: 1096 KRLRKVGDVEKNENTESAEKTSVSPLANMVGTFSSSRHIKKKKRDGERRFEDNVRTYIEE 1155

Query: 1141 EAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLLSQS 1200
            EAEV+SDATIS DEEDDKIKSSFDSFIDDRVSAS  T TQDETG  DMMA+YRRSLLSQS
Sbjct: 1156 EAEVSSDATISGDEEDDKIKSSFDSFIDDRVSASA-TSTQDETGGHDMMAIYRRSLLSQS 1215

Query: 1201 PFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQS----SEYVKMNRSPEVVTSA 1260
            PFGRLTSPL+ RV  TES+ S  KTLN+F +TL  +VNQS    SE+VKM RSPEVV S 
Sbjct: 1216 PFGRLTSPLATRV--TESDTSPDKTLNVFQTTLTGNVNQSHTMHSEHVKMKRSPEVVIST 1275

Query: 1261 TGCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEF 1307
            TG C  A EVESRK+ LTF ASESVPKL+L++ FE  V GRES++DVD         DEF
Sbjct: 1276 TGGCPSATEVESRKRNLTFCASESVPKLNLDREFELVVAGRESISDVD---------DEF 1335

BLAST of MC04g2021 vs. ExPASy TrEMBL
Match: A0A6J1FMD3 (DEAD-box ATP-dependent RNA helicase FANCM isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445272 PE=3 SV=1)

HSP 1 Score: 2029 bits (5256), Expect = 0.0
Identity = 1067/1367 (78.05%), Postives = 1156/1367 (84.56%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            DFDWEAAVKEIDVACLSGI S SSRSL+ SL+AS  ASA+N ASFP  EEN+KRG SRQ 
Sbjct: 16   DFDWEAAVKEIDVACLSGIHSASSRSLYSSLLASPHASAENSASFPSPEENEKRGTSRQS 75

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TLHRF++NPN K + KT +VEEPVQDR +VE+   L+DID+EAAKTWIYPVNVPLRDYQL
Sbjct: 76   TLHRFIINPNAKSQKKTPEVEEPVQDRALVEDSVCLVDIDQEAAKTWIYPVNVPLRDYQL 135

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN
Sbjct: 136  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 195

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA
Sbjct: 196  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 255

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCVA+RELMMVPVPLRILALTATPGSK+Q IQQIIDNLHIS L+YRDESDHD
Sbjct: 256  HRALGNYSYCVAIRELMMVPVPLRILALTATPGSKKQGIQQIIDNLHISNLQYRDESDHD 315

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            VSPYVHDRKIEL+QVAMGEDA EINNKLLE IRPFVAKLCSMG+LQ+RDYRTLSPCDLLN
Sbjct: 316  VSPYVHDRKIELIQVAMGEDAFEINNKLLEAIRPFVAKLCSMGVLQDRDYRTLSPCDLLN 375

Query: 361  SRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSFA 420
            SRDKFRQAPP  HPHIK QDVEG+FGVLITLYHIRKLLSSHGIRPASEMLEEKL +GSFA
Sbjct: 376  SRDKFRQAPPSVHPHIKYQDVEGYFGVLITLYHIRKLLSSHGIRPASEMLEEKLQRGSFA 435

Query: 421  RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSV 480
            RFMSKNDHICKVRLIMEKSL HGAPSPKLSKMMEVLVDHFKTKDP+ SRVIIFSNFRGSV
Sbjct: 436  RFMSKNDHICKVRLIMEKSLIHGAPSPKLSKMMEVLVDHFKTKDPKDSRVIIFSNFRGSV 495

Query: 481  RDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEG 540
            RDIM ALAKI DIVRATEFIGQSSGK+LKGQSQKVQQAVLEKFRAGGYNVIVAT IGEEG
Sbjct: 496  RDIMGALAKIEDIVRATEFIGQSSGKSLKGQSQKVQQAVLEKFRAGGYNVIVATCIGEEG 555

Query: 541  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIK 600
            LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRV +L+CEGSELKGYMRKQT SKSIK
Sbjct: 556  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVVVLACEGSELKGYMRKQTTSKSIK 615

Query: 601  RHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKKL 660
            +HMQNGG+NSFNFH  PRMI H I+PEVQFVK+SIKQFVRPGKKVKD+H  Q T FK KL
Sbjct: 616  KHMQNGGINSFNFHASPRMIPHTIKPEVQFVKLSIKQFVRPGKKVKDEHAVQITSFKNKL 675

Query: 661  TDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLHF 720
            T+ ET+LLLKYFHPCED W+PS+IAFPHFQTFPSRTH V HSSRTM+LIDTMQHL+GL+F
Sbjct: 676  TNTETELLLKYFHPCEDAWKPSIIAFPHFQTFPSRTHGVRHSSRTMILIDTMQHLQGLYF 735

Query: 721  SRDSEAISV------------------------SLNEVNASKAELDGPLVHPEVLPPPNP 780
            SRDSEA SV                        S+NEV+A K EL+GP+V+PEV  PPNP
Sbjct: 736  SRDSEAFSVQEKPCIRELFEASQIGKCYGGKRGSINEVSAPKTELEGPIVYPEVSAPPNP 795

Query: 781  AENNCISDYCFSKHSINI-------DHFG------------------------SKILLSS 840
             ENNC S YC  KH  NI       D  G                        SKILLSS
Sbjct: 796  TENNCSSVYCSLKHPSNIGSGFVSVDAMGEDQISRFPVFSSKEILHSNSVDACSKILLSS 855

Query: 841  -ELGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIH 900
             ELGS  VRTAQDL V+TEAV++PTTSQTKFLQ+E+L SPETD   VLEDKA+NQIE IH
Sbjct: 856  PELGSWRVRTAQDLIVRTEAVDEPTTSQTKFLQNEVLPSPETDDATVLEDKAVNQIEKIH 915

Query: 901  QTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPI 960
            Q+ +  RT FN+GD+  E PVVLEIEPQFP ADECSIIETQLSPRLTNL++SGFVPDSPI
Sbjct: 916  QSTVLKRTFFNEGDNTDEKPVVLEIEPQFPPADECSIIETQLSPRLTNLIESGFVPDSPI 975

Query: 961  DDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGE 1020
            D+CG S RQRIYE  I +FILPVQVDGEQLLKS S G ++RINCN GS AG+DVFL++GE
Sbjct: 976  DECGYS-RQRIYESAISQFILPVQVDGEQLLKSSSPGINKRINCNEGSHAGNDVFLASGE 1035

Query: 1021 VQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKPRKF 1080
             +PS+L++NDSV +KS    SPVA+ETQTP AIIASSC +EDW+LV GEKSSS+QKPRKF
Sbjct: 1036 DRPSVLEDNDSVGVKSHGFTSPVAEETQTPFAIIASSCDNEDWNLVSGEKSSSVQKPRKF 1095

Query: 1081 KRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAFIEE 1140
            KRLRK+GDVEKNEN ES   T V P+AN+VGTFSS+R  KKKKRDGERR +DNVRA+IEE
Sbjct: 1096 KRLRKVGDVEKNENTESAEKTSVSPLANMVGTFSSSRHIKKKKRDGERRFEDNVRAYIEE 1155

Query: 1141 EAEVTSDATISEDEEDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLLSQS 1200
            EAEV+SDATIS DEEDD IKSSFDSFIDDRVSAS  T TQDETG  DMMA+YRRSLLSQS
Sbjct: 1156 EAEVSSDATISGDEEDDMIKSSFDSFIDDRVSASA-TSTQDETGGHDMMAIYRRSLLSQS 1215

Query: 1201 PFGRLTSPLSARVAETESENSSGKTLNIFHSTLKEHVNQS----SEYVKMNRSPEVVTSA 1260
            PFGRLTSP + RV  TESE S  KTLN+F +TL  +VNQS    SE+VKM RSPEVV S 
Sbjct: 1216 PFGRLTSPHATRV--TESETSPDKTLNVFQTTLTGNVNQSHTMHSEHVKMKRSPEVVIST 1275

Query: 1261 TGCCSRAAEVESRKKILTFYASESVPKLSLEKLFEFEVTGRESMNDVDLNGNVLNDDDEF 1307
            TG C  A EVESR + LTF ASESVPKL+L++ FE  V GRES++DVD         DEF
Sbjct: 1276 TGGCPSATEVESRNRNLTFCASESVPKLNLDRQFELVVAGRESISDVD---------DEF 1335

BLAST of MC04g2021 vs. ExPASy TrEMBL
Match: A0A6J1JS92 (DEAD-box ATP-dependent RNA helicase FANCM isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489325 PE=3 SV=1)

HSP 1 Score: 2024 bits (5245), Expect = 0.0
Identity = 1075/1413 (76.08%), Postives = 1162/1413 (82.24%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            DFDWEAAVKEIDVACLSGI S SSRSLHPSL+AS DASA+NPASFP  EEN+KRG SRQ 
Sbjct: 16   DFDWEAAVKEIDVACLSGIHSASSRSLHPSLLASPDASAENPASFPSPEENEKRGTSRQS 75

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TLHRF+ NPN K + KT +VEEPV+DR +VE+   L+DID+EAAKTWIYPVNVPLRDYQL
Sbjct: 76   TLHRFITNPNAKSQKKTPEVEEPVKDRALVEDSVCLVDIDQEAAKTWIYPVNVPLRDYQL 135

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN
Sbjct: 136  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 195

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA
Sbjct: 196  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 255

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCVA+RELMMV VPLRILALTATPGSKQQ IQQIIDNLHIS L+YRDESDHD
Sbjct: 256  HRALGNYSYCVAIRELMMVRVPLRILALTATPGSKQQGIQQIIDNLHISNLQYRDESDHD 315

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            VSPYVHDRKIEL+QVAMGEDAVEINNKLLE IRPFVAKLCSMG+LQNRDYRTLSPCDLLN
Sbjct: 316  VSPYVHDRKIELIQVAMGEDAVEINNKLLEAIRPFVAKLCSMGILQNRDYRTLSPCDLLN 375

Query: 361  SRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSFA 420
            SRDKFRQAPPP HPHIK QDVEG+FGVLITLYHIRKLLSSHGIRPASEMLEEKL +GSFA
Sbjct: 376  SRDKFRQAPPPVHPHIKYQDVEGYFGVLITLYHIRKLLSSHGIRPASEMLEEKLQRGSFA 435

Query: 421  RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSV 480
            RFMSKNDHICKVRLIMEKSL HGAPSPKLSKMMEVLVDHFKTKDP+ SRVIIFSNFRGSV
Sbjct: 436  RFMSKNDHICKVRLIMEKSLIHGAPSPKLSKMMEVLVDHFKTKDPKDSRVIIFSNFRGSV 495

Query: 481  RDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEG 540
            RDIM ALAKI DIVRATEFIGQSSGK+LKGQSQKVQQAVLEKFRAGGYNVIVAT IGEEG
Sbjct: 496  RDIMGALAKIEDIVRATEFIGQSSGKSLKGQSQKVQQAVLEKFRAGGYNVIVATCIGEEG 555

Query: 541  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIK 600
            LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRV +L+CEGSELKGYMRKQT SKSIK
Sbjct: 556  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVVVLACEGSELKGYMRKQTTSKSIK 615

Query: 601  RHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKKL 660
            +HMQNGG+NSFNFH  PRMI H I+PEVQFVK+SIKQFVRPGKKVKDDH  Q T FK +L
Sbjct: 616  KHMQNGGINSFNFHASPRMIPHTIKPEVQFVKLSIKQFVRPGKKVKDDHAVQITSFKNQL 675

Query: 661  TDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLHF 720
            T+ ET+LLLKYFHPCED W+PSLIAFPHFQTFPSRTH V HSSRTM+LIDTMQHL+GL+F
Sbjct: 676  TNTETELLLKYFHPCEDAWKPSLIAFPHFQTFPSRTHGVRHSSRTMILIDTMQHLQGLYF 735

Query: 721  SRDSEAISV------------------------SLNEVNASKAELDGPLVHPEVLPPPNP 780
            SRDSEA SV                        S+NEVNA KAEL+GP+V+PEV  PPNP
Sbjct: 736  SRDSEAFSVQEKPCIRELFEASQIGKCYGCKRGSINEVNAPKAELEGPIVYPEVSAPPNP 795

Query: 781  AENNCISDYCFSKHSINI-------DHFG------------------------SKILLSS 840
             ENNCIS YC  KH  NI       D  G                        SKILLSS
Sbjct: 796  KENNCISVYCSLKHPSNIGSGFVSVDAMGEDQISRFPVFSSKEILHSNSVDACSKILLSS 855

Query: 841  E-LGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIH 900
              LGS  VRTAQDL V+TEAV++PTTSQTKFLQ+E+L SPETD   VLEDKA+NQIE IH
Sbjct: 856  PGLGSWRVRTAQDLIVRTEAVDEPTTSQTKFLQNEVLPSPETDDATVLEDKAVNQIEKIH 915

Query: 901  QTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPI 960
            Q+ +  RT FN+GD+  E PVV EIEPQFP ADEC+IIETQLSPRLTNL++SGFVPDSPI
Sbjct: 916  QSTVLKRTFFNEGDNTDEKPVVSEIEPQFPPADECNIIETQLSPRLTNLIESGFVPDSPI 975

Query: 961  DDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGE 1020
            D+CG S RQRIYE  I +FIL VQVDGEQLLKS S G ++RINCN GS AG+D+FL++GE
Sbjct: 976  DECGYS-RQRIYESAISQFILHVQVDGEQLLKSSSPGINKRINCNEGSHAGNDIFLASGE 1035

Query: 1021 VQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKPRKF 1080
             +PS+L++NDSV +KS A  SPVA+ETQTP AIIASSC +EDW+LV GEKSSS+QKPRKF
Sbjct: 1036 DRPSVLEDNDSVGVKSHAFTSPVAEETQTPFAIIASSCDNEDWNLVSGEKSSSVQKPRKF 1095

Query: 1081 KRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDG-------------- 1140
            KRLRK+GDVEKNEN ES   T V P+AN+VGTFSS+R  KKKKRDG              
Sbjct: 1096 KRLRKVGDVEKNENTESAEKTSVSPLANMVGTFSSSRHIKKKKRDGIFFSHSSIFGISLS 1155

Query: 1141 --------------------------------ERRLDDNVRAFIEEEAEVTSDATISEDE 1200
                                            ERR +DNVR +IEEEAEV+SDATIS DE
Sbjct: 1156 VLNISEDLIEHTFKQSKSDQVATCQYQLLHLGERRFEDNVRTYIEEEAEVSSDATISGDE 1215

Query: 1201 EDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLLSQSPFGRLTSPLSARVA 1260
            EDDKIKSSFDSFIDDRVSAS  T TQDETG  DMMA+YRRSLLSQSPFGRLTSPL+ RV 
Sbjct: 1216 EDDKIKSSFDSFIDDRVSASA-TSTQDETGGHDMMAIYRRSLLSQSPFGRLTSPLATRV- 1275

Query: 1261 ETESENSSGKTLNIFHSTLKEHVNQS----SEYVKMNRSPEVVTSATGCCSRAAEVESRK 1307
             TES+ S  KTLN+F +TL  +VNQS    SE+VKM RSPEVV S TG C  A EVESRK
Sbjct: 1276 -TESDTSPDKTLNVFQTTLTGNVNQSHTMHSEHVKMKRSPEVVISTTGGCPSATEVESRK 1335

BLAST of MC04g2021 vs. ExPASy TrEMBL
Match: A0A6J1FGT9 (DEAD-box ATP-dependent RNA helicase FANCM isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445272 PE=3 SV=1)

HSP 1 Score: 2007 bits (5199), Expect = 0.0
Identity = 1067/1413 (75.51%), Postives = 1156/1413 (81.81%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            DFDWEAAVKEIDVACLSGI S SSRSL+ SL+AS  ASA+N ASFP  EEN+KRG SRQ 
Sbjct: 16   DFDWEAAVKEIDVACLSGIHSASSRSLYSSLLASPHASAENSASFPSPEENEKRGTSRQS 75

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TLHRF++NPN K + KT +VEEPVQDR +VE+   L+DID+EAAKTWIYPVNVPLRDYQL
Sbjct: 76   TLHRFIINPNAKSQKKTPEVEEPVQDRALVEDSVCLVDIDQEAAKTWIYPVNVPLRDYQL 135

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN
Sbjct: 136  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 195

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA
Sbjct: 196  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 255

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCVA+RELMMVPVPLRILALTATPGSK+Q IQQIIDNLHIS L+YRDESDHD
Sbjct: 256  HRALGNYSYCVAIRELMMVPVPLRILALTATPGSKKQGIQQIIDNLHISNLQYRDESDHD 315

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            VSPYVHDRKIEL+QVAMGEDA EINNKLLE IRPFVAKLCSMG+LQ+RDYRTLSPCDLLN
Sbjct: 316  VSPYVHDRKIELIQVAMGEDAFEINNKLLEAIRPFVAKLCSMGVLQDRDYRTLSPCDLLN 375

Query: 361  SRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSFA 420
            SRDKFRQAPP  HPHIK QDVEG+FGVLITLYHIRKLLSSHGIRPASEMLEEKL +GSFA
Sbjct: 376  SRDKFRQAPPSVHPHIKYQDVEGYFGVLITLYHIRKLLSSHGIRPASEMLEEKLQRGSFA 435

Query: 421  RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSV 480
            RFMSKNDHICKVRLIMEKSL HGAPSPKLSKMMEVLVDHFKTKDP+ SRVIIFSNFRGSV
Sbjct: 436  RFMSKNDHICKVRLIMEKSLIHGAPSPKLSKMMEVLVDHFKTKDPKDSRVIIFSNFRGSV 495

Query: 481  RDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEG 540
            RDIM ALAKI DIVRATEFIGQSSGK+LKGQSQKVQQAVLEKFRAGGYNVIVAT IGEEG
Sbjct: 496  RDIMGALAKIEDIVRATEFIGQSSGKSLKGQSQKVQQAVLEKFRAGGYNVIVATCIGEEG 555

Query: 541  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIK 600
            LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRV +L+CEGSELKGYMRKQT SKSIK
Sbjct: 556  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVVVLACEGSELKGYMRKQTTSKSIK 615

Query: 601  RHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKKL 660
            +HMQNGG+NSFNFH  PRMI H I+PEVQFVK+SIKQFVRPGKKVKD+H  Q T FK KL
Sbjct: 616  KHMQNGGINSFNFHASPRMIPHTIKPEVQFVKLSIKQFVRPGKKVKDEHAVQITSFKNKL 675

Query: 661  TDVETKLLLKYFHPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLHF 720
            T+ ET+LLLKYFHPCED W+PS+IAFPHFQTFPSRTH V HSSRTM+LIDTMQHL+GL+F
Sbjct: 676  TNTETELLLKYFHPCEDAWKPSIIAFPHFQTFPSRTHGVRHSSRTMILIDTMQHLQGLYF 735

Query: 721  SRDSEAISV------------------------SLNEVNASKAELDGPLVHPEVLPPPNP 780
            SRDSEA SV                        S+NEV+A K EL+GP+V+PEV  PPNP
Sbjct: 736  SRDSEAFSVQEKPCIRELFEASQIGKCYGGKRGSINEVSAPKTELEGPIVYPEVSAPPNP 795

Query: 781  AENNCISDYCFSKHSINI-------DHFG------------------------SKILLSS 840
             ENNC S YC  KH  NI       D  G                        SKILLSS
Sbjct: 796  TENNCSSVYCSLKHPSNIGSGFVSVDAMGEDQISRFPVFSSKEILHSNSVDACSKILLSS 855

Query: 841  -ELGSCSVRTAQDLTVQTEAVNKPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIH 900
             ELGS  VRTAQDL V+TEAV++PTTSQTKFLQ+E+L SPETD   VLEDKA+NQIE IH
Sbjct: 856  PELGSWRVRTAQDLIVRTEAVDEPTTSQTKFLQNEVLPSPETDDATVLEDKAVNQIEKIH 915

Query: 901  QTAISNRTLFNKGDSASEMPVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPI 960
            Q+ +  RT FN+GD+  E PVVLEIEPQFP ADECSIIETQLSPRLTNL++SGFVPDSPI
Sbjct: 916  QSTVLKRTFFNEGDNTDEKPVVLEIEPQFPPADECSIIETQLSPRLTNLIESGFVPDSPI 975

Query: 961  DDCGNSSRQRIYEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGE 1020
            D+CG S RQRIYE  I +FILPVQVDGEQLLKS S G ++RINCN GS AG+DVFL++GE
Sbjct: 976  DECGYS-RQRIYESAISQFILPVQVDGEQLLKSSSPGINKRINCNEGSHAGNDVFLASGE 1035

Query: 1021 VQPSLLKENDSVIIKSRASISPVADETQTPLAIIASSCGSEDWHLVPGEKSSSIQKPRKF 1080
             +PS+L++NDSV +KS    SPVA+ETQTP AIIASSC +EDW+LV GEKSSS+QKPRKF
Sbjct: 1036 DRPSVLEDNDSVGVKSHGFTSPVAEETQTPFAIIASSCDNEDWNLVSGEKSSSVQKPRKF 1095

Query: 1081 KRLRKIGDVEKNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDG-------------- 1140
            KRLRK+GDVEKNEN ES   T V P+AN+VGTFSS+R  KKKKRDG              
Sbjct: 1096 KRLRKVGDVEKNENTESAEKTSVSPLANMVGTFSSSRHIKKKKRDGIFFSHSSIFGISLS 1155

Query: 1141 --------------------------------ERRLDDNVRAFIEEEAEVTSDATISEDE 1200
                                            ERR +DNVRA+IEEEAEV+SDATIS DE
Sbjct: 1156 VLNISEDLIEHTFKQSKSDQVATCQYQLLHLGERRFEDNVRAYIEEEAEVSSDATISGDE 1215

Query: 1201 EDDKIKSSFDSFIDDRVSASGGTCTQDETGRPDMMAVYRRSLLSQSPFGRLTSPLSARVA 1260
            EDD IKSSFDSFIDDRVSAS  T TQDETG  DMMA+YRRSLLSQSPFGRLTSP + RV 
Sbjct: 1216 EDDMIKSSFDSFIDDRVSASA-TSTQDETGGHDMMAIYRRSLLSQSPFGRLTSPHATRV- 1275

Query: 1261 ETESENSSGKTLNIFHSTLKEHVNQS----SEYVKMNRSPEVVTSATGCCSRAAEVESRK 1307
             TESE S  KTLN+F +TL  +VNQS    SE+VKM RSPEVV S TG C  A EVESR 
Sbjct: 1276 -TESETSPDKTLNVFQTTLTGNVNQSHTMHSEHVKMKRSPEVVISTTGGCPSATEVESRN 1335

BLAST of MC04g2021 vs. TAIR 10
Match: AT1G35530.2 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 691/1424 (48.53%), Postives = 869/1424 (61.03%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            +FDWEAAVKEID+ACL    ++SS S H + +A       NP   P      K  A RQ 
Sbjct: 15   EFDWEAAVKEIDLACLKTTNASSSSSSHFTPLA-------NP---PITANLTKPPAKRQS 74

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TL +F+     K  N  +  E  V D     +   L+ ID EAAKTWIYP  VPLRDYQ 
Sbjct: 75   TLDKFIGRTEHKPENHQVVSECGVND----NDNSPLVGIDPEAAKTWIYPGFVPLRDYQF 134

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFP+GKIVF APSRPLV+QQIEACHN
Sbjct: 135  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPQGKIVFAAPSRPLVMQQIEACHN 194

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTID+TG   P+KRA  WKSKRVFFVTPQVLEKDIQSGTCL  YLVCLVIDEA
Sbjct: 195  IVGIPQEWTIDLTGQTCPSKRAFLWKSKRVFFVTPQVLEKDIQSGTCLTNYLVCLVIDEA 254

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCV VRELM VP+ LRILALTATPGSK QAIQ IIDNL ISTLEYR+ESDHD
Sbjct: 255  HRALGNYSYCVVVRELMAVPIQLRILALTATPGSKTQAIQGIIDNLQISTLEYRNESDHD 314

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            V PYVHDRK+E+++V +G+DA +++ +L  VIRP+  +L + G+  NRD +TLSP ++L 
Sbjct: 315  VCPYVHDRKLEVIEVPLGQDADDVSKRLFHVIRPYAVRLKNFGVNLNRDIQTLSPHEVLM 374

Query: 361  SRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSFA 420
            +RDKFRQAP P  PH+   DVE  F  LITLYHIRKLLSSHGIRPA EMLEEKL +G FA
Sbjct: 375  ARDKFRQAPLPGLPHVNHGDVESCFAALITLYHIRKLLSSHGIRPAYEMLEEKLKEGPFA 434

Query: 421  RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSV 480
            R MSKN+ I   +L+M++ LSHGAPSPKLSKM+E+LVDHFK KDP+ SRVIIFSNFRGSV
Sbjct: 435  RLMSKNEDIRMTKLLMQQRLSHGAPSPKLSKMLEILVDHFKVKDPKTSRVIIFSNFRGSV 494

Query: 481  RDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEG 540
            RDIM+AL+ IGD+V+ATEFIGQSSGKTLKGQSQK+QQAVLEKFRAGG+NVIVATSIGEEG
Sbjct: 495  RDIMNALSNIGDMVKATEFIGQSSGKTLKGQSQKIQQAVLEKFRAGGFNVIVATSIGEEG 554

Query: 541  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGR-VDILSCEGSELKGYMRKQTNSKSI 600
            LDIMEVDLVICFDAN+SP+RMIQRMGRTGRK++GR + +L+CEGSE   YMRKQ + ++I
Sbjct: 555  LDIMEVDLVICFDANVSPLRMIQRMGRTGRKNNGRPLLVLACEGSEKNSYMRKQASGRAI 614

Query: 601  KRHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKK 660
            K+HM+NGG NSFNFH  PRMI H  +PEVQ V+ SIKQFV  GKK+++++  +T  F+KK
Sbjct: 615  KKHMRNGGTNSFNFHPSPRMIPHVYKPEVQHVEFSIKQFVPRGKKLQEEYATETPAFQKK 674

Query: 661  LTDVETKLLLKYF-HPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGL 720
            LT  ET +L KY+ +P E+  R SLIAFPHFQT PS+ H VMHS +T +LID MQHL+  
Sbjct: 675  LTPAETHMLAKYYNNPDEEKLRVSLIAFPHFQTLPSKVHKVMHSRQTGMLIDAMQHLQEP 734

Query: 721  HFSRDSEAISVSLNEVNASKAELDGPL--------VH--------------PEVLPPPNP 780
             FS  S++           + ELD  L        +H               +V  P + 
Sbjct: 735  TFSEQSKSFFTEFRAPLGEREELDTGLRVTNDPKDLHSVRDLEVNTSQRKAKQVESPTST 794

Query: 781  --------AENNCISDYCFSKHSINIDHFGSKI-----------LLSSELGSCSVRTAQD 840
                     E++    Y FS    ++D  G+             +L S+         Q 
Sbjct: 795  LETTEKDYEESSPTHRYLFSSECASVDTLGNVFVMPVPLLFFPNVLESDNTPLPKTEKQH 854

Query: 841  LTVQTEAVN-KPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIHQTAISNRTLFNK 900
                T  ++  P  +  K  QD I    +        D A   +ET H     N T   +
Sbjct: 855  SCRNTSHIDLVPVDTSEKHRQDNISCKLKE---RFSPDGASETLET-HSLVKRNSTRVGE 914

Query: 901  GDSASEM-PVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPIDDCGNSSRQRI 960
             D A+ +  +VL  +      D+C  +E  LSPRLTN +KSG VP+SP+ D G ++R+  
Sbjct: 915  DDVANSVGEIVLSSD-----EDDCEGLE--LSPRLTNFIKSGIVPESPVYDQGEANREED 974

Query: 961  YEFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGEVQPSLLKENDS 1020
             EF  P+   P++   E   +S       +  CN  +       L T + +  L    + 
Sbjct: 975  LEF--PQLSSPMRFSNELAGESSFPERKVQHKCNDYNIVSTTTELRTPQKEVGLANGTEC 1034

Query: 1021 VIIKSRASISPVADETQTPLAII--ASSCGSEDWHLVPGEKSSSIQKPRKFKRLRKIGDV 1080
            +      ++SP+ ++ +TPLA +   +S   +DW +  GEK  ++++PRK KRLR++GD 
Sbjct: 1035 L------AVSPIPEDWRTPLANLTNTNSSARKDWRVSSGEKLETLRQPRKLKRLRRLGDC 1094

Query: 1081 EK--NENMESMVGT--------------LVPPMANIVGTFSSTR---PTKKKKRDGERRL 1140
                 EN   +                 +    A    T SS      TKKKK      +
Sbjct: 1095 SSAVKENYPGITEADHIRSRSRGKKHIRVKWKSAESWFTLSSVAFALVTKKKK----MIM 1154

Query: 1141 DDNVRAFIEEEAEVTSDATISEDEEDDKIKSSF-DSFIDDRVSASGGTCTQDETGRPDMM 1200
            DD+V+ FI+EEAEV+S A +S DE +D    SF DSFIDD    +    TQ E+G+ DMM
Sbjct: 1155 DDDVQVFIDEEAEVSSGAEMSADENEDVTGDSFEDSFIDDGTMPTAN--TQAESGKVDMM 1214

Query: 1201 AVY--------------------------RRSLLSQSPF--------GRLTSPLSA--RV 1260
            AVY                          RRSLLSQSP             SP SA    
Sbjct: 1215 AVYRYIQPKISFFYCEVNELIKNHKVSFHRRSLLSQSPLPARFRDLAASSLSPYSAGPLT 1274

Query: 1261 AETESENSSGKTLNIFHSTLKEHVNQSSEYVKMNRSPEVVTSATGCCSRAAEVESRKKIL 1308
               ES + S K+L+    T K   ++S++   M  +  VV  ++         +SRK+  
Sbjct: 1275 RINESRSDSDKSLSSLR-TPKTTNSESNQDAMMIGNLSVVQISS---------DSRKRKF 1334

BLAST of MC04g2021 vs. TAIR 10
Match: AT1G35530.1 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 1026.5 bits (2653), Expect = 1.8e-299
Identity = 659/1404 (46.94%), Postives = 831/1404 (59.19%), Query Frame = 0

Query: 1    DFDWEAAVKEIDVACLSGIPSTSSRSLHPSLVASSDASADNPASFPFLEENKKRGASRQP 60
            +FDWEAAVKEID+ACL    ++SS S H + +A       NP   P      K  A RQ 
Sbjct: 15   EFDWEAAVKEIDLACLKTTNASSSSSSHFTPLA-------NP---PITANLTKPPAKRQS 74

Query: 61   TLHRFMVNPNTKLRNKTLDVEEPVQDRGIVEELDSLIDIDEEAAKTWIYPVNVPLRDYQL 120
            TL +F+     K  N  +  E  V D     +   L+ ID EAAKTWIYP  VPLRDYQ 
Sbjct: 75   TLDKFIGRTEHKPENHQVVSECGVND----NDNSPLVGIDPEAAKTWIYPGFVPLRDYQF 134

Query: 121  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGKIVFTAPSRPLVLQQIEACHN 180
            AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFP+GKIVF APSRPLV+QQIEACHN
Sbjct: 135  AITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPQGKIVFAAPSRPLVMQQIEACHN 194

Query: 181  IVGIPQEWTIDMTGLINPTKRACFWKSKRVFFVTPQVLEKDIQSGTCLVKYLVCLVIDEA 240
            IVGIPQEWTID+TG   P+KRA  WKSKRVFFVTPQVLEKDIQSGTCL  YLVCLVIDEA
Sbjct: 195  IVGIPQEWTIDLTGQTCPSKRAFLWKSKRVFFVTPQVLEKDIQSGTCLTNYLVCLVIDEA 254

Query: 241  HRALGNYSYCVAVRELMMVPVPLRILALTATPGSKQQAIQQIIDNLHISTLEYRDESDHD 300
            HRALGNYSYCV VRELM VP+ LRILALTATPGSK QAIQ IIDNL ISTLEYR+ESDHD
Sbjct: 255  HRALGNYSYCVVVRELMAVPIQLRILALTATPGSKTQAIQGIIDNLQISTLEYRNESDHD 314

Query: 301  VSPYVHDRKIELMQVAMGEDAVEINNKLLEVIRPFVAKLCSMGMLQNRDYRTLSPCDLLN 360
            V PYVHDRK+E+++V +G+DA +++ +L  VIRP+  +L + G+  NRD +TLSP ++L 
Sbjct: 315  VCPYVHDRKLEVIEVPLGQDADDVSKRLFHVIRPYAVRLKNFGVNLNRDIQTLSPHEVLM 374

Query: 361  SRDKFRQAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSHGIRPASEMLEEKLHQGSFA 420
            +RDKFRQAP P  PH+   DVE  F  LITLYHIRKLLSSHGIRPA EMLEEKL +G FA
Sbjct: 375  ARDKFRQAPLPGLPHVNHGDVESCFAALITLYHIRKLLSSHGIRPAYEMLEEKLKEGPFA 434

Query: 421  RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSV 480
            R MSKN+ I   +L+M++ LSHGAPSPKLSKM+E+LVDHFK KDP+ SRVIIFSNFRGSV
Sbjct: 435  RLMSKNEDIRMTKLLMQQRLSHGAPSPKLSKMLEILVDHFKVKDPKTSRVIIFSNFRGSV 494

Query: 481  RDIMDALAKIGDIVRATEFIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEG 540
            RDIM+AL+ IGD+V+ATEFIGQSSGKTLKGQSQK+QQAVLEKFRAGG+NVIVATSIGEEG
Sbjct: 495  RDIMNALSNIGDMVKATEFIGQSSGKTLKGQSQKIQQAVLEKFRAGGFNVIVATSIGEEG 554

Query: 541  LDIMEVDLVICFDANISPIRMIQRMGRTGRKHDGRVDILSCEGSELKGYMRKQTNSKSIK 600
            LDIMEVDLVICFDAN+SP+RMIQRMGRTGRK++GR                         
Sbjct: 555  LDIMEVDLVICFDANVSPLRMIQRMGRTGRKNNGR------------------------- 614

Query: 601  RHMQNGGMNSFNFHGCPRMISHAIRPEVQFVKMSIKQFVRPGKKVKDDHTAQTTLFKKKL 660
                               I H  +PEVQ V+ SIKQFV  GKK+++++  +T  F+KKL
Sbjct: 615  -------------------IPHVYKPEVQHVEFSIKQFVPRGKKLQEEYATETPAFQKKL 674

Query: 661  TDVETKLLLKYF-HPCEDTWRPSLIAFPHFQTFPSRTHAVMHSSRTMVLIDTMQHLEGLH 720
            T  ET +L KY+ +P E+  R SLIAFPHFQT PS+ H VMHS +T +LID MQHL+   
Sbjct: 675  TPAETHMLAKYYNNPDEEKLRVSLIAFPHFQTLPSKVHKVMHSRQTGMLIDAMQHLQEPT 734

Query: 721  FSRDSEAISVSLNEVNASKAELDGPL--------VH--------------PEVLPPPNP- 780
            FS  S++           + ELD  L        +H               +V  P +  
Sbjct: 735  FSEQSKSFFTEFRAPLGEREELDTGLRVTNDPKDLHSVRDLEVNTSQRKAKQVESPTSTL 794

Query: 781  -------AENNCISDYCFSKHSINIDHFGSKI-----------LLSSELGSCSVRTAQDL 840
                    E++    Y FS    ++D  G+             +L S+         Q  
Sbjct: 795  ETTEKDYEESSPTHRYLFSSECASVDTLGNVFVMPVPLLFFPNVLESDNTPLPKTEKQHS 854

Query: 841  TVQTEAVN-KPTTSQTKFLQDEILSSPETDGVNVLEDKAMNQIETIHQTAISNRTLFNKG 900
               T  ++  P  +  K  QD I    +        D A   +ET H     N T   + 
Sbjct: 855  CRNTSHIDLVPVDTSEKHRQDNISCKLKE---RFSPDGASETLET-HSLVKRNSTRVGED 914

Query: 901  DSASEM-PVVLEIEPQFPLADECSIIETQLSPRLTNLVKSGFVPDSPIDDCGNSSRQRIY 960
            D A+ +  +VL  +      D+C  +E  LSPRLTN +KSG VP+SP+ D G ++R+   
Sbjct: 915  DVANSVGEIVLSSD-----EDDCEGLE--LSPRLTNFIKSGIVPESPVYDQGEANREEDL 974

Query: 961  EFTIPEFILPVQVDGEQLLKSPSLGTSERINCNGGSCAGDDVFLSTGEVQPSLLKENDSV 1020
            EF  P+   P++   E   +S       +  CN  +       L T + +  L    + +
Sbjct: 975  EF--PQLSSPMRFSNELAGESSFPERKVQHKCNDYNIVSTTTELRTPQKEVGLANGTECL 1034

Query: 1021 IIKSRASISPVADETQTPLAII--ASSCGSEDWHLVPGEKSSSIQKPRKFKRLRKIGDVE 1080
                  ++SP+ ++ +TPLA +   +S   +DW +  GEK  ++++PRK KRLR++GD  
Sbjct: 1035 ------AVSPIPEDWRTPLANLTNTNSSARKDWRVSSGEKLETLRQPRKLKRLRRLGD-- 1094

Query: 1081 KNENMESMVGTLVPPMANIVGTFSSTRPTKKKKRDGERRLDDNVRAFIEEEAEVTSDATI 1140
                  S V    P +       S +R  K  +   +  +DD+V+ FI+EEAEV+S A +
Sbjct: 1095 ----CSSAVKENYPGITEADHIRSRSRGKKHIRGKKKMIMDDDVQVFIDEEAEVSSGAEM 1154

Query: 1141 SEDEEDDKIKSSF-DSFIDDRVSASGGTCTQDETGRPDMMAVY----------------- 1200
            S DE +D    SF DSFIDD    +    TQ E+G+ DMMAVY                 
Sbjct: 1155 SADENEDVTGDSFEDSFIDDGTMPTAN--TQAESGKVDMMAVYRYIQPKISFFYCEVNEL 1214

Query: 1201 ---------RRSLLSQSPF--------GRLTSPLSA--RVAETESENSSGKTLNIFHSTL 1260
                     RRSLLSQSP             SP SA       ES + S K+L+    T 
Sbjct: 1215 IKNHKVSFHRRSLLSQSPLPARFRDLAASSLSPYSAGPLTRINESRSDSDKSLSSLR-TP 1274

Query: 1261 KEHVNQSSEYVKMNRSPEVVTSATGCCSRAAEVESRKKILTFYASESVPKLSLEKLF--E 1308
            K   ++S++   M  +  VV  ++         +SRK+  +   S + P ++LE  F   
Sbjct: 1275 KTTNSESNQDAMMIGNLSVVQISS---------DSRKRKFSLCNSANAPVINLESKFAAH 1323

BLAST of MC04g2021 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 85.1 bits (209), Expect = 4.5e-16
Identity = 121/571 (21.19%), Postives = 221/571 (38.70%), Query Frame = 0

Query: 116 RDYQLAITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGK----IVFTAPSRPLV 175
           R YQL + + A   NT+  L TG GKTLIA +++ +  +           VF  P  PLV
Sbjct: 250 RRYQLDVLEQAKAKNTIAFLETGAGKTLIAILLIKSVHKDLMSQNRKMLSVFLVPKVPLV 309

Query: 176 LQQIEACHNIVGIPQEWTIDMTGLINPTKRACFW---------KSKRVFFVTPQVLEKDI 235
            QQ E   N        T    G         FW         +SK+V  +T Q+L   +
Sbjct: 310 YQQAEVIRN-------QTCFQVGHYCGEMGQDFWDSRRWQREFESKQVLVMTAQILLNIL 369

Query: 236 QSGTCLVKYLVCLVIDEAHRALGNYSYCVAVRELM-MVPVPLR--ILALTATPGSKQQAI 295
           +     ++ +  L++DE H A+  + Y + + E     P   R  I  +TA+P + +   
Sbjct: 370 RHSIIRMETIDLLILDECHHAVKKHPYSLVMSEFYHTTPKDKRPAIFGMTASPVNLKGVS 429

Query: 296 QQIIDNLHISTLEYRDESDHDVSPYVHDRKIELMQVAMGEDAVEINNK------LLEVIR 355
            Q+   + I  LE + +S       + DRK     V M  + V   +K      L E I+
Sbjct: 430 SQVDCAIKIRNLETKLDS---TVCTIKDRKELEKHVPMPSEIVVEYDKAATMWSLHETIK 489

Query: 356 PFVAKLCSMGMLQNRDYRTLSPCDLLNSRD---------------------------KFR 415
             +A +       +R     S    + +RD                           K R
Sbjct: 490 QMIAAVEEAAQASSRK----SKWQFMGARDAGAKDELRQVYGVSERTESDGAANLIHKLR 549

Query: 416 -------------------------QAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSH 475
                                    Q+    +  +  +  E +   +++L     L  + 
Sbjct: 550 AINYTLAELGQWCAYKVGQSFLSALQSDERVNFQVDVKFQESYLSEVVSLLQCELLEGAA 609

Query: 476 GIRPASEM-------LEEKLHQGSFA--RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKM 535
             + A+E+         +++ +G       +S  +H+ +V   +  +++ G  +PK+  +
Sbjct: 610 AEKVAAEVGKPENGNAHDEMEEGELPDDPVVSGGEHVDEV---IGAAVADGKVTPKVQSL 669

Query: 536 MEVLVDHFKTKDPQYSRVIIFSNFRGSVRDIMDALAKIGDI--VRATEFIGQSSGKTLKG 595
           +++L+ +  T D    R I+F     +   +    A++  +  +R    IG ++ + +K 
Sbjct: 670 IKLLLKYQHTAD---FRAIVFVERVVAALVLPKVFAELPSLSFIRCASMIGHNNSQEMKS 729

Query: 596 QSQKVQQAVLEKFRAGGYNVIVATSIGEEGLDIMEVDLVICFDANISPIRMIQRMGRTGR 602
                 Q  + KFR G   ++VATS+ EEGLDI + ++V+ FD   + +  IQ  GR  +
Sbjct: 730 SQ---MQDTISKFRDGHVTLLVATSVAEEGLDIRQCNVVMRFDLAKTVLAYIQSRGRARK 789

BLAST of MC04g2021 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 85.1 bits (209), Expect = 4.5e-16
Identity = 121/571 (21.19%), Postives = 221/571 (38.70%), Query Frame = 0

Query: 116 RDYQLAITKTALFSNTLVALPTGLGKTLIAAVVMYNYFRWFPEGK----IVFTAPSRPLV 175
           R YQL + + A   NT+  L TG GKTLIA +++ +  +           VF  P  PLV
Sbjct: 250 RRYQLDVLEQAKAKNTIAFLETGAGKTLIAILLIKSVHKDLMSQNRKMLSVFLVPKVPLV 309

Query: 176 LQQIEACHNIVGIPQEWTIDMTGLINPTKRACFW---------KSKRVFFVTPQVLEKDI 235
            QQ E   N        T    G         FW         +SK+V  +T Q+L   +
Sbjct: 310 YQQAEVIRN-------QTCFQVGHYCGEMGQDFWDSRRWQREFESKQVLVMTAQILLNIL 369

Query: 236 QSGTCLVKYLVCLVIDEAHRALGNYSYCVAVRELM-MVPVPLR--ILALTATPGSKQQAI 295
           +     ++ +  L++DE H A+  + Y + + E     P   R  I  +TA+P + +   
Sbjct: 370 RHSIIRMETIDLLILDECHHAVKKHPYSLVMSEFYHTTPKDKRPAIFGMTASPVNLKGVS 429

Query: 296 QQIIDNLHISTLEYRDESDHDVSPYVHDRKIELMQVAMGEDAVEINNK------LLEVIR 355
            Q+   + I  LE + +S       + DRK     V M  + V   +K      L E I+
Sbjct: 430 SQVDCAIKIRNLETKLDS---TVCTIKDRKELEKHVPMPSEIVVEYDKAATMWSLHETIK 489

Query: 356 PFVAKLCSMGMLQNRDYRTLSPCDLLNSRD---------------------------KFR 415
             +A +       +R     S    + +RD                           K R
Sbjct: 490 QMIAAVEEAAQASSRK----SKWQFMGARDAGAKDELRQVYGVSERTESDGAANLIHKLR 549

Query: 416 -------------------------QAPPPAHPHIKCQDVEGFFGVLITLYHIRKLLSSH 475
                                    Q+    +  +  +  E +   +++L     L  + 
Sbjct: 550 AINYTLAELGQWCAYKVGQSFLSALQSDERVNFQVDVKFQESYLSEVVSLLQCELLEGAA 609

Query: 476 GIRPASEM-------LEEKLHQGSFA--RFMSKNDHICKVRLIMEKSLSHGAPSPKLSKM 535
             + A+E+         +++ +G       +S  +H+ +V   +  +++ G  +PK+  +
Sbjct: 610 AEKVAAEVGKPENGNAHDEMEEGELPDDPVVSGGEHVDEV---IGAAVADGKVTPKVQSL 669

Query: 536 MEVLVDHFKTKDPQYSRVIIFSNFRGSVRDIMDALAKIGDI--VRATEFIGQSSGKTLKG 595
           +++L+ +  T D    R I+F     +   +    A++  +  +R    IG ++ + +K 
Sbjct: 670 IKLLLKYQHTAD---FRAIVFVERVVAALVLPKVFAELPSLSFIRCASMIGHNNSQEMKS 729

Query: 596 QSQKVQQAVLEKFRAGGYNVIVATSIGEEGLDIMEVDLVICFDANISPIRMIQRMGRTGR 602
                 Q  + KFR G   ++VATS+ EEGLDI + ++V+ FD   + +  IQ  GR  +
Sbjct: 730 SQ---MQDTISKFRDGHVTLLVATSVAEEGLDIRQCNVVMRFDLAKTVLAYIQSRGRARK 789

BLAST of MC04g2021 vs. TAIR 10
Match: AT5G20320.1 (dicer-like 4 )

HSP 1 Score: 76.6 bits (187), Expect = 1.6e-13
Identity = 116/489 (23.72%), Postives = 211/489 (43.15%), Query Frame = 0

Query: 116 RDYQLAITKTALFSNTLVALPTGLGKTLIAAVVMY--NYFRWFPEGKI-VFTAPSRPLVL 175
           R YQ+ + K A   N +V L TG GKT IA +++Y   +    P+  + +F AP+  LV 
Sbjct: 125 RRYQVELCKKATEENVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVE 184

Query: 176 QQIEACHNIVGIPQEWTIDMTGLINPTKRACFWK----SKRVFFVTPQVLEKDIQSGTCL 235
           QQ +   + V           G     K    W+    +  V  +TPQ+L  ++Q     
Sbjct: 185 QQAKVIADSVNFK---VAIHCGGKRIVKSHSEWEREIAANEVLVMTPQILLHNLQHCFIK 244

Query: 236 VKYLVCLVIDEAHRA--LGNYSYCVAVR-----ELMMVPVPLRILALTATPGSKQQAIQQ 295
           ++ +  L+ DE H A    N+ Y   ++     E +  P   RI  +TA+P   + + Q 
Sbjct: 245 MECISLLIFDECHHAQQQSNHPYAEIMKVFYKSESLQRP---RIFGMTASPVVGKGSFQS 304

Query: 296 IIDNLHISTLE-------YRDESDHDVSPYVHDRKIELMQVA-----MGEDAVEINNKLL 355
              +  I++LE       Y  ES+  +  +V    +++           +  +   N L 
Sbjct: 305 ENLSKSINSLENLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENMLE 364

Query: 356 EVIRPFVAKLCSMGMLQNRDYRTLSPCDLLNSRDKFRQAPPPAHPHIKCQDVE-GFFGVL 415
           ++ +  +A L    ++     +TL     L  R         +H ++    +  G +G +
Sbjct: 365 DIKQRCLASL--KLLIDTHQTQTLLSMKRLLKR---------SHDNLIYTLLNLGLWGAI 424

Query: 416 ITLYHIRKLLSSHGIR-------PASEMLEEKLHQGSFARFMSKNDHICKVRLIMEKSLS 475
                  +L S H ++       P S++ +  L   + A             L+   +L 
Sbjct: 425 QAAK--IQLNSDHNVQDEPVGKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALK 484

Query: 476 HGAPSPKLSKMMEVLVDHFKTKDPQYSRVIIFSNFRGSVRDIMDALAKIGDIVRATE--- 535
               S KL +++++L   F+ +   + + IIF N   + R +   L  + +++R+ +   
Sbjct: 485 EPLFSRKLVQLIKIL-SVFRLE--PHMKCIIFVNRIVTARTLSCILNNL-ELLRSWKSDF 544

Query: 536 FIGQSSGKTLKGQSQKVQQAVLEKFRAGGYNVIVATSIGEEGLDIMEVDLVICFDANISP 568
            +G SSG  LK  S++  + +L++F++   N++VAT +GEEGLDI    LVI +D   + 
Sbjct: 545 LVGLSSG--LKSMSRRSMETILKRFQSKELNLLVATKVGEEGLDIQTCCLVIRYDLPETV 588