Homology
BLAST of MC04g2002 vs. ExPASy Swiss-Prot
Match:
F4IYF8 (Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760 PE=2 SV=1)
HSP 1 Score: 1001.1 bits (2587), Expect = 7.7e-291
Identity = 549/890 (61.69%), Postives = 670/890 (75.28%), Query Frame = 0
Query: 5 SEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQVF-IP 64
+E S ITLGGKGSSLSSSSVY +A G A VRIDSSA+ER S +RN S K F IP
Sbjct: 3 AERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFS----TRNVPSIKRSSFGIP 62
Query: 65 NFLTREEARASLAVLLNKLIIS-----SSSGIRSVIPVLIAETLNSKPETLQFESLDVTD 124
LT EE RASLAVLLNKLI+S SSS RSV+P+ I E LN K E+L+ +DVT+
Sbjct: 63 QGLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVTE 122
Query: 125 EELSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFAS 184
E V ++SC L GIC+++DH+S LS I D+VAA+SCE ++AD+++F L+DSGDG
Sbjct: 123 GENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNGD 182
Query: 185 KEEVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFG 244
K+ +GVA D+KVLLNG K GK+EI SKIP HG R K VH+ R ELNS +K G
Sbjct: 183 KDVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKGG 242
Query: 245 KGGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 304
K GS ++G +++ T LL + +LG CS R KL +EN+K L ++ C
Sbjct: 243 KTGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKC---LSEKICV 302
Query: 305 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGEL-LGKG 364
NE+LK YKL L++ Y F H +N L VW+IV EA+AAF + GGEL + K
Sbjct: 303 ENENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQKS 362
Query: 365 QDVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKD 424
D D E ++ KK + +KKAV+GKGTS+V+Q IKDRL + + SL +
Sbjct: 363 GDAD-KEESKTDKKKKKNEKKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQCGEQ 422
Query: 425 LLSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVF 484
+L+ +P+ FD+LL K+KEIVESNE+RRLPKLPKGTRDFAKEQMA+R+KAFSII+ VF
Sbjct: 423 ILNLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQNVF 482
Query: 485 ERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGI 544
+RHGATALDTP FELRETLMGKYGEDSKL+YD+ADQGGELCSLRYDLTVPFARYVAMNGI
Sbjct: 483 KRHGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNGI 542
Query: 545 TSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQ 604
TSFKRYQIAKVYRRDNPSKGR+REFYQCDFDIAG +E MGPDFE++KILTELLDEL IG
Sbjct: 543 TSFKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGD 602
Query: 605 YEIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEK 664
YE+KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSF+Q+K+EMVEEKGL+ EIA++
Sbjct: 603 YEVKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADR 662
Query: 665 IGDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSL 724
IG+FVKE+G PLELLSKL+QE L N+ S +AL EL I+F AL++SKC +++VFDLSL
Sbjct: 663 IGNFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSL 722
Query: 725 ARGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIME 784
ARGLDYYTGVI+EAV G +VGSI AGGRYDNLIGMFG+KQVPAVG+SLGIERVF IME
Sbjct: 723 ARGLDYYTGVIFEAVCI-GAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVFNIME 782
Query: 785 QLLKDQNQTTRATKTEVLVSILGDD-LTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAK 844
+L + Q Q R T+T+VLVSI+ D+ L +AAEL S++W AK+NAE+LV+KR KH +RAK
Sbjct: 783 ELNEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAK 842
Query: 845 ESRIPWIVFLGEREVSEGIVKLKNV----ETFEEVTISRSNIIDELKKRL 883
ES IPW+V +GE+E+S V LK + E E+ T +R ++ELKK L
Sbjct: 843 ESGIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883
BLAST of MC04g2002 vs. ExPASy Swiss-Prot
Match:
P93422 (Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0150900 PE=2 SV=2)
HSP 1 Score: 763.8 bits (1971), Expect = 2.1e-219
Identity = 422/760 (55.53%), Postives = 534/760 (70.26%), Query Frame = 0
Query: 138 LDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKEEVGVANDMKVLLNGSKL 197
+D ++ L +ADAVAA+SCEA+R DV+AF + SGDG ++K+E VA D+K+LL GSKL
Sbjct: 36 IDCCASPLVRVADAVAALSCEAARGDVAAFDVPTSGDGLSAKDEADVAADVKMLLFGSKL 95
Query: 198 VGK---IEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKGGSLSS--GTEDSVR 257
VG + +F+K+P +G RE + +H+++R+ELN+ +K GK ++ + G E+++
Sbjct: 96 VGAAGGADAASFTKVPTVNGIFREAVRALHARVRIELNAPVKLGKRDAVQTGEGKEEALV 155
Query: 258 TALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSNESLKKEYKLVSE 317
A + + K S+ R +L ++ + ++ L ++ + LK ++ +
Sbjct: 156 ALATQLARPVQAMLKLSVARARLCVARIDDAELRKKLTDGVE-----IDDLK---GMLGK 215
Query: 318 LALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDVDINEV------- 377
+ +D + V L+ I+AWEA A IE + K Q + NE
Sbjct: 216 VTIDSDAVSVLRGVYNSLLKFRDILAWEAAVAMAVIEMDSSIEKPQACEENEAGSSTENP 275
Query: 378 ---NEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLSFL 437
EK + KKK LGKGTS V+ L++D L G + L +L
Sbjct: 276 HASGEKPKGDKKSKKKKTLGKGTSAVLMLLRD-LVTNGKEVLSVNSALLAEWGTELSLLF 335
Query: 438 DPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGA 497
DPK +L+ K+KEIVE+NE RRLPK+PKGTRDF KEQMAIR+ AFSII GVF+ HGA
Sbjct: 336 DPKCPRLVSLVDKVKEIVETNEVRRLPKIPKGTRDFGKEQMAIREHAFSIITGVFKMHGA 395
Query: 498 TALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKR 557
+LDTP FELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMN I+S KR
Sbjct: 396 VSLDTPVFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNNISSLKR 455
Query: 558 YQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEIKL 617
YQIAKVYRRDNPSKGR+REFYQCDFDIAG YE M PDFEVIK+LTELLD+L IG YEIKL
Sbjct: 456 YQIAKVYRRDNPSKGRYREFYQCDFDIAGVYETMEPDFEVIKVLTELLDQLDIGTYEIKL 515
Query: 618 NHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFV 677
NHRKLLDGMLEICGVPPEKFRT+CSSIDKLDKQ+F+Q+K+E+V+EKG++ E A+KIGD V
Sbjct: 516 NHRKLLDGMLEICGVPPEKFRTVCSSIDKLDKQTFEQVKKELVDEKGISNETADKIGDLV 575
Query: 678 KERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARGLD 737
K RG PLE+L +L++E + N GS AL ELEILF AL+K+ + K+VFDLSLARGLD
Sbjct: 576 KTRGPPLEVLLELRKEGSKFMGNAGSVTALNELEILFKALDKANAIGKIVFDLSLARGLD 635
Query: 738 YYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKD 797
YYTGVIYEAVFKG TQVGSIAAGGRYDNL+GMF KQVPAVGVSLGIERVF IMEQ +
Sbjct: 636 YYTGVIYEAVFKGTTQVGSIAAGGRYDNLVGMFSGKQVPAVGVSLGIERVFAIMEQQEME 695
Query: 798 QNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRIPW 857
+NQ RAT+TEVLVSI+G DL AAEL SE+W+A + AEF + R+ H+ A +S IPW
Sbjct: 696 KNQ-IRATETEVLVSIIGKDLILAAELVSELWNAGIKAEFKLTTRIQNHLKYATQSGIPW 755
Query: 858 IVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRL 883
+V +GE E+S G VKLKN+ +E + R+ LK++L
Sbjct: 756 MVLVGESEISSGKVKLKNLAASQEEEVDRTEFAQVLKQKL 785
BLAST of MC04g2002 vs. ExPASy Swiss-Prot
Match:
Q2KI84 (Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1)
HSP 1 Score: 473.4 bits (1217), Expect = 5.6e-132
Identity = 243/449 (54.12%), Postives = 329/449 (73.27%), Query Frame = 0
Query: 450 KLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYD 509
K PKGTRD++ QMA+R+K F +I F+RHGA +DTP FEL+ETL GKYGEDSKLIYD
Sbjct: 57 KTPKGTRDYSPRQMAVREKVFDVIISCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYD 116
Query: 510 LADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYRRDNP--SKGRFREFYQCDF 569
L DQGGEL SLRYDLTVPFARY+AMN +T+ KRY IAKVYRRDNP ++GR+REFYQCDF
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176
Query: 570 DIAGQYEKMGPDFEVIKILTELLDELSIGQYEIKLNHRKLLDGMLEICGVPPEKFRTICS 629
DIAGQ++ M PD E +KI+ E+L L IG + +K+N R++LDGM ICGVP KFRTICS
Sbjct: 177 DIAGQFDPMLPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVPDSKFRTICS 236
Query: 630 SIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHPLELLSKLKQEQCPLLQNKG 689
S+DKLDK S++++K EMV EKGL E+A++IGD+V++ G + L+ +L Q+ L QNK
Sbjct: 237 SVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-GVSLVEQLLQDP-KLSQNKQ 296
Query: 690 SFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVF-----KGGTQ---V 749
+ + LG+L++LF L DK+ FDLSLARGLDYYTGVIYEAV + G + V
Sbjct: 297 ALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQPPARAGEEPLGV 356
Query: 750 GSIAAGGRYDNLIGMFGSK--QVPAVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLV- 809
GS+AAGGRYD L+GMF K +VP VG+S+G+ER+F I+EQ L+ + R T+T+VLV
Sbjct: 357 GSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKVRTTETQVLVA 416
Query: 810 SILGDDLTQAAELASEIWSAKLNAEFLV--NKRVMKHIDRAKESRIPWIVFLGEREVSEG 869
S L + +L SE+W A + AE L N +++ + +E+ IP + +GE+E+ +G
Sbjct: 417 SAQKKLLEERLKLISELWDAGIKAELLYKKNPKLLNQLQYCEETGIPLVAIIGEQELKDG 476
Query: 870 IVKLKNVETFEEVTISRSNIIDELKKRLS 884
++KL++V + EEV + R ++++E+K+R S
Sbjct: 477 VIKLRSVASREEVDVRREDLVEEIKRRTS 503
BLAST of MC04g2002 vs. ExPASy Swiss-Prot
Match:
E9QI36 (Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2)
HSP 1 Score: 470.7 bits (1210), Expect = 3.6e-131
Identity = 246/459 (53.59%), Postives = 331/459 (72.11%), Query Frame = 0
Query: 450 KLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYD 509
K KGTRD+ +QMAIR+K F+II F+RHGA +D+P FEL+ETL GKYGEDSKLIYD
Sbjct: 57 KTAKGTRDYNPKQMAIREKVFNIIINCFKRHGAETIDSPVFELKETLTGKYGEDSKLIYD 116
Query: 510 LADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYRRDNP--SKGRFREFYQCDF 569
L DQGGEL SLRYDLTVPFARY+AMN IT+ KRY IAKVYRRDNP ++GR+REFYQCDF
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKITNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176
Query: 570 DIAGQYEKMGPDFEVIKILTELLDELSIGQYEIKLNHRKLLDGMLEICGVPPEKFRTICS 629
DIAGQY+ M PD E +K++ E+L EL +G + IK+N R++LDGM ICGVP EKFRTICS
Sbjct: 177 DIAGQYDAMIPDAECLKLVYEILSELDLGDFRIKVNDRRILDGMFAICGVPDEKFRTICS 236
Query: 630 SIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHPLELLSKLKQEQCPLLQNKG 689
++DKLDK +++++K+EMV EKGL+ E+A++I D+V +G +L +L Q+ L Q+K
Sbjct: 237 TVDKLDKLAWEEVKKEMVNEKGLSEEVADRIRDYVSMQGGK-DLAERLLQDP-KLSQSKQ 296
Query: 690 SFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVF-------------- 749
+ + ++++LF+ LE + DKVVFDLSLARGLDYYTGVIYEA+
Sbjct: 297 ACAGITDMKLLFSYLELFQITDKVVFDLSLARGLDYYTGVIYEAILTQANPAPASTPAEQ 356
Query: 750 ----KGGTQVGSIAAGGRYDNLIGMFGSK--QVPAVGVSLGIERVFVIMEQLLKDQNQTT 809
G VGS+A GGRYD L+GMF K +VP VGVS+GIERVF IMEQ + ++
Sbjct: 357 NGAEDAGVSVGSVAGGGRYDGLVGMFDPKGRKVPCVGVSIGIERVFSIMEQKAELSSEKV 416
Query: 810 RATKTEVLV-SILGDDLTQAAELASEIWSAKLNAEFLV--NKRVMKHIDRAKESRIPWIV 869
R T+T+VLV S + L + +L +E+W+A + AE L N +++ + +++ IP +
Sbjct: 417 RTTETQVLVASAQKNLLEERLKLTAELWNAGIKAEVLYKKNPKLLSQLQHCEDTGIPLVA 476
Query: 870 FLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLS 884
LGE+E+ +G+VKL+NV + EEV + R+ ++DE+KKR S
Sbjct: 477 ILGEQELKDGVVKLRNVASREEVDVPRAELVDEVKKRTS 513
BLAST of MC04g2002 vs. ExPASy Swiss-Prot
Match:
Q61035 (Histidine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Hars1 PE=1 SV=2)
HSP 1 Score: 468.8 bits (1205), Expect = 1.4e-130
Identity = 245/466 (52.58%), Postives = 338/466 (72.53%), Query Frame = 0
Query: 433 LKKIKEIVESNESRR--LPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAF 492
L K+K + +E ++ + K PKGTRD++ QMA+R+K F +I F+RHGA +DTP F
Sbjct: 38 LLKLKAQLGQDEGKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVF 97
Query: 493 ELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYR 552
EL+ETL GKYGEDSKLIYDL DQGGEL SLRYDLTVPFARY+AMN +T+ KRY IAKVYR
Sbjct: 98 ELKETLTGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYR 157
Query: 553 RDNP--SKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEIKLNHRKLL 612
RDNP ++GR+REFYQCDFDIAGQ++ M PD E +KI+ E+L L IG + +K+N R++L
Sbjct: 158 RDNPAMTRGRYREFYQCDFDIAGQFDPMIPDAECLKIMCEILSSLQIGNFLVKVNDRRIL 217
Query: 613 DGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHP 672
DGM +CGVP KFRTICSS+DKLDK S++++K EMV EKGL E+A++IGD+V++ G
Sbjct: 218 DGMFAVCGVPDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-G 277
Query: 673 LELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVI 732
+ L+ +L Q+ L QNK + + LG+L++LF L DK+ FDLSLARGLDYYTGVI
Sbjct: 278 VSLVEQLLQDP-KLSQNKQAVEGLGDLKLLFEYLILFGIDDKISFDLSLARGLDYYTGVI 337
Query: 733 YEAVF-----KGGTQ---VGSIAAGGRYDNLIGMFGSK--QVPAVGVSLGIERVFVIMEQ 792
YEAV + G + VGSIAAGGRYD L+GMF K +VP VG+S+G+ER+F I+EQ
Sbjct: 338 YEAVLLQMPTQAGEEPLGVGSIAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQ 397
Query: 793 LLKDQNQTTRATKTEVLV-SILGDDLTQAAELASEIWSAKLNAEFLV--NKRVMKHIDRA 852
L+ + R T+T+VLV S L + +L SE+W A + AE L N +++ +
Sbjct: 398 RLEASEEKVRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYC 457
Query: 853 KESRIPWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKR 882
+E+ IP + +GE+E+ +G++KL++V + EEV + R ++++E+++R
Sbjct: 458 EEAGIPLVAIIGEQELKDGVIKLRSVASREEVDVRREDLVEEIRRR 501
BLAST of MC04g2002 vs. NCBI nr
Match:
XP_022152462.1 (histidine--tRNA ligase, cytoplasmic [Momordica charantia])
HSP 1 Score: 1684 bits (4360), Expect = 0.0
Identity = 884/884 (100.00%), Postives = 884/884 (100.00%), Query Frame = 0
Query: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV
Sbjct: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
Query: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
Query: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN
Sbjct: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV
Sbjct: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS
Sbjct: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
BLAST of MC04g2002 vs. NCBI nr
Match:
XP_022954606.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >XP_022954615.1 histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata])
HSP 1 Score: 1561 bits (4041), Expect = 0.0
Identity = 814/884 (92.08%), Postives = 847/884 (95.81%), Query Frame = 0
Query: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1 MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
Query: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
IPN+LTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61 VIPNYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
Query: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSAALSSIADAVA ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
EVGVANDMKVLLNGSKLVGKIE GA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
GSLSSGTED+VRTALLSFAA+LWDLGKCSL RGKLI SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAILWDLGKCSLDRGKLIFDSNSDDNMKASLAGLLDRECPSN 300
Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
D+N NEKVV K+EKKKKAVLGKGTSVVVQLIKDRLQGKGG LENSVKD+LS
Sbjct: 361 DMNRANEKVVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLG------LENSVKDILS 420
Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSF 540
Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IGQYEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600
Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660
Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
FVKERGHPLELL+KLKQEQ PLLQNKGS AL ELEILF+ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARG 720
Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840
Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of MC04g2002 vs. NCBI nr
Match:
KAG6602728.1 (Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1560 bits (4040), Expect = 0.0
Identity = 815/884 (92.19%), Postives = 848/884 (95.93%), Query Frame = 0
Query: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1 MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
Query: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
IP++LTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61 VIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
Query: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSAALSSIADAVA ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
EVGVANDMKVLLNGSKLVGKIE GA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
GSLSSGTED+VRTALLSFAA+LWDLGKCSL RGKLIL SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
D+N NEKVV K+EKKKKAVLGKGTSVVVQLIKDRLQGKGG LENSVKDLLS
Sbjct: 361 DMNRANEKVVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLG------LENSVKDLLS 420
Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSF 540
Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IGQYEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600
Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660
Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
FVKERGHPLELL+KLKQEQ PLLQNKGS AL ELEILF+ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARG 720
Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840
Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of MC04g2002 vs. NCBI nr
Match:
XP_023536635.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1553 bits (4022), Expect = 0.0
Identity = 812/884 (91.86%), Postives = 845/884 (95.59%), Query Frame = 0
Query: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1 MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
Query: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
IP++LT EEARASL VLLNKLIISSSSGIRSVIPVLIAETLNS PETLQFESLDVTDEE
Sbjct: 61 VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120
Query: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSAALSSIADAVA+ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
EVGVANDMKVLLNGSKLVGKIE GA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
GSLSSGTED+VRTALLSFAA+LWDLGKCSL RGKLIL SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
D+N NEK V K+EKKKKAVLGKGTSVVVQLIKDRLQGKGG LENSVKDLLS
Sbjct: 361 DMNRANEKGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLG------LENSVKDLLS 420
Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSF 540
Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IGQYEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600
Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660
Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
FVKERGHPLELL+KLKQEQ PLLQNKGS AL ELEILF ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARG 720
Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840
Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of MC04g2002 vs. NCBI nr
Match:
XP_022990844.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita maxima])
HSP 1 Score: 1552 bits (4018), Expect = 0.0
Identity = 811/884 (91.74%), Postives = 849/884 (96.04%), Query Frame = 0
Query: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1 MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
Query: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
IP++LTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61 VIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
Query: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSAALSSIADAVA ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
EVGVANDMKVLLNGSKLVGKIE GA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
GS+SSGTED+VRTALLSFAA+LWDLGKCSL RGKLIL SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSMSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
D+N NEKV+ K+EKKKKAVLGKGTSVVVQLIKDRLQG+ +G LG LENSVKDLLS
Sbjct: 361 DMNRANEKVLNKSEKKKKAVLGKGTSVVVQLIKDRLQGE-----SGDLG-LENSVKDLLS 420
Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARY+AMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYIAMNGITSF 540
Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IGQYEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600
Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660
Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
FVKERGHPLELL+KLKQEQ PLLQNKGS AL ELEILF+ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARG 720
Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ L
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQHL 780
Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840
Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of MC04g2002 vs. ExPASy TrEMBL
Match:
A0A6J1DGB6 (Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1)
HSP 1 Score: 1684 bits (4360), Expect = 0.0
Identity = 884/884 (100.00%), Postives = 884/884 (100.00%), Query Frame = 0
Query: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV
Sbjct: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
Query: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
Query: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN
Sbjct: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV
Sbjct: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS
Sbjct: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
BLAST of MC04g2002 vs. ExPASy TrEMBL
Match:
A0A6J1GRC1 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456820 PE=3 SV=1)
HSP 1 Score: 1561 bits (4041), Expect = 0.0
Identity = 814/884 (92.08%), Postives = 847/884 (95.81%), Query Frame = 0
Query: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1 MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
Query: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
IPN+LTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61 VIPNYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
Query: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSAALSSIADAVA ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
EVGVANDMKVLLNGSKLVGKIE GA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
GSLSSGTED+VRTALLSFAA+LWDLGKCSL RGKLI SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAILWDLGKCSLDRGKLIFDSNSDDNMKASLAGLLDRECPSN 300
Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
D+N NEKVV K+EKKKKAVLGKGTSVVVQLIKDRLQGKGG LENSVKD+LS
Sbjct: 361 DMNRANEKVVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLG------LENSVKDILS 420
Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSF 540
Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IGQYEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600
Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660
Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
FVKERGHPLELL+KLKQEQ PLLQNKGS AL ELEILF+ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARG 720
Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840
Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of MC04g2002 vs. ExPASy TrEMBL
Match:
A0A6J1JT52 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487614 PE=3 SV=1)
HSP 1 Score: 1552 bits (4018), Expect = 0.0
Identity = 811/884 (91.74%), Postives = 849/884 (96.04%), Query Frame = 0
Query: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1 MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60
Query: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
IP++LTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61 VIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
Query: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSAALSSIADAVA ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
EVGVANDMKVLLNGSKLVGKIE GA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240
Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
GS+SSGTED+VRTALLSFAA+LWDLGKCSL RGKLIL SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSMSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300
Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360
Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
D+N NEKV+ K+EKKKKAVLGKGTSVVVQLIKDRLQG+ +G LG LENSVKDLLS
Sbjct: 361 DMNRANEKVLNKSEKKKKAVLGKGTSVVVQLIKDRLQGE-----SGDLG-LENSVKDLLS 420
Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARY+AMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYIAMNGITSF 540
Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IGQYEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600
Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660
Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
FVKERGHPLELL+KLKQEQ PLLQNKGS AL ELEILF+ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARG 720
Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ L
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQHL 780
Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840
Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of MC04g2002 vs. ExPASy TrEMBL
Match:
A0A6J1FE95 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111444724 PE=3 SV=1)
HSP 1 Score: 1526 bits (3952), Expect = 0.0
Identity = 798/886 (90.07%), Postives = 844/886 (95.26%), Query Frame = 0
Query: 1 MADPS-EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQ 60
MADP+ E+SVITLGGKGSSL+S+SVYAIAHGF+LVRIDSS+LERL+SSSN+ NAAS KH
Sbjct: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
Query: 61 VFIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
+F+P+FLTR+EARASL VLLNKLIIS+SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASK 180
ELSVF+QSCY+LNGICALLDHQSAALSSIADAVA+ISCEASRADVSAF L+DSGDGFASK
Sbjct: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
Query: 181 EEVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGK 240
EEVG+ANDMKV LNGSKLVGKIE GA S IPK HGCLREQAKLVHSKMRVELNS +K GK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GG-SLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 300
GG SLSSGTED+VRT LLSFAA+LWDLGKCSL R KLIL SN +EN+KASL GLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
Query: 301 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQ 360
S+ESLK+EY+LVSEL LD++Y EFVHTVNVL TVWKIVAWEAIAAF+TIEGGEL+GKGQ
Sbjct: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDL 420
DVDIN NEKVVKKNEKKKKAVLGKG+SVVVQLIKD+LQGKGGG LGSLEN VKDL
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGG-----LGSLENLVKDL 420
Query: 421 LSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480
LSFLDPKAS FDNLL+KIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE
Sbjct: 421 LSFLDPKASGFDNLLRKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480
Query: 481 RHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540
RHGATALDTPAFELRETLMGKYGEDSKL+YDLADQGGELCSLRYDLTVPFARYVAMNGIT
Sbjct: 481 RHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540
Query: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQY 600
SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IGQY
Sbjct: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQY 600
Query: 601 EIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKI 660
EIKLNHR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAE+I
Sbjct: 601 EIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERI 660
Query: 661 GDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLA 720
GDFVKERGHPLELLSKLKQEQ PLLQNKGS DAL ELEILF+ALEKS CVDKVVFDLSLA
Sbjct: 661 GDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLA 720
Query: 721 RGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780
RGLDYYTGVIYEAVFKGGTQ+GSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ
Sbjct: 721 RGLDYYTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780
Query: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKES 840
LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAK++AEFLVNKRVMKHIDRAKE+
Sbjct: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEA 840
Query: 841 RIPWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
RIPWIVFLGERE SEGIVKLKNVETFEEVT+SRSNIIDELKKRL+P
Sbjct: 841 RIPWIVFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLTP 881
BLAST of MC04g2002 vs. ExPASy TrEMBL
Match:
A0A6J1JV84 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111489180 PE=3 SV=1)
HSP 1 Score: 1523 bits (3942), Expect = 0.0
Identity = 799/886 (90.18%), Postives = 841/886 (94.92%), Query Frame = 0
Query: 1 MADPS-EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQ 60
MA P+ E+SVITLGGKGSSL+S+SVYAIAHGF+LVRIDSS+LERL+SSSN+ NAAS KH
Sbjct: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
Query: 61 VFIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
+F+P++LTREEARASL VLLNKLIIS SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASK 180
ELSVF+QSCYVL+GICALLDHQSAALSSIADAVA+ISCEASRADVSAF LMDSGDGFASK
Sbjct: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
Query: 181 EEVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGK 240
EEVG+ANDMKV LNGSKLVGKIE GA SKIPK HGCLREQAKLVHSKMRVELNS +K GK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GG-SLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 300
GG SLSSGTED+VRT LLSFAA+LWDLGKCSL R KLIL SN +EN+KASL GLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
Query: 301 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQ 360
SNESLK+EYKLVSEL LD++Y EFVHTVNVL TVWKIVAWEAIAAF+TIEGGEL+GKGQ
Sbjct: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDL 420
DVDIN NEKVVKKNEKKKKAVLGKG+SVVVQLIKD+LQGKGGG LGSLEN VK +
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGG-----LGSLENLVKAI 420
Query: 421 LSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480
LSFLDPKAS FDNLLKKIKEIVESN SRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE
Sbjct: 421 LSFLDPKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480
Query: 481 RHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540
RHGATALDTPAFELRETLMGKYGEDSKL+YDLADQGGELCSLRYDLTVPFARYVAMNGIT
Sbjct: 481 RHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540
Query: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQY 600
SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IGQY
Sbjct: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQY 600
Query: 601 EIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKI 660
EIKLNHR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAE+I
Sbjct: 601 EIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERI 660
Query: 661 GDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLA 720
GDFVKERGHPLELLSKLKQEQ PLLQNKGS DAL ELEILF+ALEKS CVDKVVFDLSLA
Sbjct: 661 GDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLA 720
Query: 721 RGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780
RGLDYYTGVIYEAVFKGGTQ+GSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ
Sbjct: 721 RGLDYYTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780
Query: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKES 840
LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAK++AEFLVNKRVMKHIDRAKE+
Sbjct: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEA 840
Query: 841 RIPWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
RIPWIVFLGERE SEGIVKLKNVETFEEVT+SRSNIIDELKKRL+P
Sbjct: 841 RIPWIVFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 881
BLAST of MC04g2002 vs. TAIR 10
Match:
AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 1001.1 bits (2587), Expect = 5.5e-292
Identity = 549/890 (61.69%), Postives = 670/890 (75.28%), Query Frame = 0
Query: 5 SEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQVF-IP 64
+E S ITLGGKGSSLSSSSVY +A G A VRIDSSA+ER S +RN S K F IP
Sbjct: 3 AERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFS----TRNVPSIKRSSFGIP 62
Query: 65 NFLTREEARASLAVLLNKLIIS-----SSSGIRSVIPVLIAETLNSKPETLQFESLDVTD 124
LT EE RASLAVLLNKLI+S SSS RSV+P+ I E LN K E+L+ +DVT+
Sbjct: 63 QGLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVTE 122
Query: 125 EELSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFAS 184
E V ++SC L GIC+++DH+S LS I D+VAA+SCE ++AD+++F L+DSGDG
Sbjct: 123 GENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNGD 182
Query: 185 KEEVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFG 244
K+ +GVA D+KVLLNG K GK+EI SKIP HG R K VH+ R ELNS +K G
Sbjct: 183 KDVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKGG 242
Query: 245 KGGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 304
K GS ++G +++ T LL + +LG CS R KL +EN+K L ++ C
Sbjct: 243 KTGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKC---LSEKICV 302
Query: 305 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGEL-LGKG 364
NE+LK YKL L++ Y F H +N L VW+IV EA+AAF + GGEL + K
Sbjct: 303 ENENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQKS 362
Query: 365 QDVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKD 424
D D E ++ KK + +KKAV+GKGTS+V+Q IKDRL + + SL +
Sbjct: 363 GDAD-KEESKTDKKKKKNEKKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQCGEQ 422
Query: 425 LLSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVF 484
+L+ +P+ FD+LL K+KEIVESNE+RRLPKLPKGTRDFAKEQMA+R+KAFSII+ VF
Sbjct: 423 ILNLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQNVF 482
Query: 485 ERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGI 544
+RHGATALDTP FELRETLMGKYGEDSKL+YD+ADQGGELCSLRYDLTVPFARYVAMNGI
Sbjct: 483 KRHGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNGI 542
Query: 545 TSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQ 604
TSFKRYQIAKVYRRDNPSKGR+REFYQCDFDIAG +E MGPDFE++KILTELLDEL IG
Sbjct: 543 TSFKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGD 602
Query: 605 YEIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEK 664
YE+KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSF+Q+K+EMVEEKGL+ EIA++
Sbjct: 603 YEVKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADR 662
Query: 665 IGDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSL 724
IG+FVKE+G PLELLSKL+QE L N+ S +AL EL I+F AL++SKC +++VFDLSL
Sbjct: 663 IGNFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSL 722
Query: 725 ARGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIME 784
ARGLDYYTGVI+EAV G +VGSI AGGRYDNLIGMFG+KQVPAVG+SLGIERVF IME
Sbjct: 723 ARGLDYYTGVIFEAVCI-GAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVFNIME 782
Query: 785 QLLKDQNQTTRATKTEVLVSILGDD-LTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAK 844
+L + Q Q R T+T+VLVSI+ D+ L +AAEL S++W AK+NAE+LV+KR KH +RAK
Sbjct: 783 ELNEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAK 842
Query: 845 ESRIPWIVFLGEREVSEGIVKLKNV----ETFEEVTISRSNIIDELKKRL 883
ES IPW+V +GE+E+S V LK + E E+ T +R ++ELKK L
Sbjct: 843 ESGIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883
BLAST of MC04g2002 vs. TAIR 10
Match:
AT3G46100.1 (Histidyl-tRNA synthetase 1 )
HSP 1 Score: 157.9 bits (398), Expect = 3.7e-38
Identity = 122/431 (28.31%), Postives = 213/431 (49.42%), Query Frame = 0
Query: 452 PKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSK-LIYDL 511
PKGTRDF E M +R F+ + V +G +D P E + K GE+ + +Y
Sbjct: 72 PKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCF 131
Query: 512 ADQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRFREFYQCDF 571
D+G +LR +LT AR V G + K + I + +R + ++GR RE YQ +
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAIGQCWRYERMTRGRRREHYQWNM 191
Query: 572 DIAGQYEKMGPDFEVIKILTELLDELSIGQYEI--KLNHRKLLDGMLEICGVPPEKFRTI 631
DI G ++ + E+I + + I ++ K++ RK+L +L+ GVP + F +
Sbjct: 192 DIIG-VPQVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRV 251
Query: 632 CSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHPLELLSKLKQEQCPLLQN 691
C IDK++K D+IK+E+ G++ + E++ L++LS + +
Sbjct: 252 CIIIDKIEKIPIDEIKKEL-GFTGISEDAIEQL----------LQVLSVKSLDDLEDIIG 311
Query: 692 KGSFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQVGSIAAG 751
G+ +A+ +L+ LF+ EK + + FD S+ RGL YYTG+++E + G ++ +I G
Sbjct: 312 -GAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEGFDRKG-KLRAICGG 371
Query: 752 GRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLVSILGDDLT- 811
GRYD L+ +G PA G G VI+E L + + E +V L DL
Sbjct: 372 GRYDRLLSTYGGDDFPACGFGFGDA---VIVELLKEKDLLPELGQEVENIVCALDKDLQG 431
Query: 812 QAAELASEIWSAKLNAEFLVNKRVMKHI-DRAKESRIPWIVFLGEREVSEGIVKLKNVET 871
AA +A+ + + ++ + +K + RA +V +G+ E +G V +K + +
Sbjct: 432 AAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEWEDGSVSVKVLSS 485
Query: 872 FEEVTISRSNI 875
E+ + S++
Sbjct: 492 GEQFQVKLSDL 485
BLAST of MC04g2002 vs. TAIR 10
Match:
AT3G59410.1 (protein kinase family protein )
HSP 1 Score: 116.3 bits (290), Expect = 1.2e-25
Identity = 115/446 (25.78%), Postives = 200/446 (44.84%), Query Frame = 0
Query: 465 IRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDL 524
+R I + VF +H A L+ + L+ + S+ L GG++ L Y+L
Sbjct: 796 LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 855
Query: 525 TVPFARYVAMNGITSFKRYQIAKVYRR------DNPSKGRFREFYQCDFDIAGQYEKMGP 584
+PF ++++N +SFKRY+I+ VYRR NP Q DFDI G +
Sbjct: 856 RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 915
Query: 585 DFEVIKILTELLDEL-SIGQYEIKLNHRKLLDGMLEICGVPPE---KFRTICSSIDKLDK 644
+ EV+K++ ++ + G +I LNH LLD + G+ E K + S + L
Sbjct: 916 EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRP 975
Query: 645 QSFDQ------IKREMVEEKGLTVEIAEKIGDFVKE-RGHPLELLSKLKQEQCPLLQNKG 704
QS ++ I+R++++E L + ++ G + L +L+ L ++
Sbjct: 976 QSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRG---ALRADRP 1035
Query: 705 SFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQVGS------ 764
+ AL EL L L + + V D+ + Y+ + ++ G+
Sbjct: 1036 TRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVL 1095
Query: 765 IAAGGRYDNLIGMFGSKQ----VP-AVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLV 824
+A GGRYD L+ ++ +P AVGVSL +E +F + L D T VLV
Sbjct: 1096 LAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIF---QHLPMDLRPIRNEVSTSVLV 1155
Query: 825 SILGDD--LTQAAELASEIWSAKLNAEFL--VNKRVMKHIDRAKESRIPWIVFLGEREVS 876
G L Q EL +E+W + AEF+ + + + + A E I +V + E V+
Sbjct: 1156 CSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVA 1215
BLAST of MC04g2002 vs. TAIR 10
Match:
AT3G59410.2 (protein kinase family protein )
HSP 1 Score: 116.3 bits (290), Expect = 1.2e-25
Identity = 115/446 (25.78%), Postives = 200/446 (44.84%), Query Frame = 0
Query: 465 IRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDL 524
+R I + VF +H A L+ + L+ + S+ L GG++ L Y+L
Sbjct: 820 LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 879
Query: 525 TVPFARYVAMNGITSFKRYQIAKVYRR------DNPSKGRFREFYQCDFDIAGQYEKMGP 584
+PF ++++N +SFKRY+I+ VYRR NP Q DFDI G +
Sbjct: 880 RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 939
Query: 585 DFEVIKILTELLDEL-SIGQYEIKLNHRKLLDGMLEICGVPPE---KFRTICSSIDKLDK 644
+ EV+K++ ++ + G +I LNH LLD + G+ E K + S + L
Sbjct: 940 EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRP 999
Query: 645 QSFDQ------IKREMVEEKGLTVEIAEKIGDFVKE-RGHPLELLSKLKQEQCPLLQNKG 704
QS ++ I+R++++E L + ++ G + L +L+ L ++
Sbjct: 1000 QSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRG---ALRADRP 1059
Query: 705 SFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQVGS------ 764
+ AL EL L L + + V D+ + Y+ + ++ G+
Sbjct: 1060 TRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVL 1119
Query: 765 IAAGGRYDNLIGMFGSKQ----VP-AVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLV 824
+A GGRYD L+ ++ +P AVGVSL +E +F + L D T VLV
Sbjct: 1120 LAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIF---QHLPMDLRPIRNEVSTSVLV 1179
Query: 825 SILGDD--LTQAAELASEIWSAKLNAEFL--VNKRVMKHIDRAKESRIPWIVFLGEREVS 876
G L Q EL +E+W + AEF+ + + + + A E I +V + E V+
Sbjct: 1180 CSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVA 1239
BLAST of MC04g2002 vs. TAIR 10
Match:
AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 87.4 bits (215), Expect = 6.2e-17
Identity = 58/187 (31.02%), Postives = 92/187 (49.20%), Query Frame = 0
Query: 452 PKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSK-LIYDL 511
PKGTRDF E M +R F+ + V G +D P E + K GE+ + +Y
Sbjct: 72 PKGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEVDFPVLETEALFIRKAGEEIRDQLYCF 131
Query: 512 ADQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRFREFYQCDF 571
D+G +LR +LT AR V G + K + + + +R + ++GR RE YQ +
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAVGQCWRYERMTRGRRREHYQWNM 191
Query: 572 DIAGQYEKMGPDFEVIKILTELLDELSIGQYEI--KLNHRKLLDGMLEICGVPPEKFRTI 631
DI G E + + E+I + + I ++ K++ RK+L L+ GVP + F +
Sbjct: 192 DIIGVPE-VTAEAELISSIMTFFKRIGITASDVGFKVSSRKVLQEFLKKYGVPEDLFGRV 251
Query: 632 CSSIDKL 633
C IDK+
Sbjct: 252 CFIIDKV 257
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4IYF8 | 7.7e-291 | 61.69 | Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760... | [more] |
| P93422 | 2.1e-219 | 55.53 | Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=... | [more] |
| Q2KI84 | 5.6e-132 | 54.12 | Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1 | [more] |
| E9QI36 | 3.6e-131 | 53.59 | Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2 | [more] |
| Q61035 | 1.4e-130 | 52.58 | Histidine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Hars1 PE=1 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022152462.1 | 0.0 | 100.00 | histidine--tRNA ligase, cytoplasmic [Momordica charantia] | [more] |
| XP_022954606.1 | 0.0 | 92.08 | histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >XP_022954615.1 hi... | [more] |
| KAG6602728.1 | 0.0 | 92.19 | Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. soro... | [more] |
| XP_023536635.1 | 0.0 | 91.86 | histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] | [more] |
| XP_022990844.1 | 0.0 | 91.74 | histidine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DGB6 | 0.0 | 100.00 | Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1 | [more] |
| A0A6J1GRC1 | 0.0 | 92.08 | Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456820 PE=3 SV=1 | [more] |
| A0A6J1JT52 | 0.0 | 91.74 | Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487614 PE=3 SV=1 | [more] |
| A0A6J1FE95 | 0.0 | 90.07 | Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111444724 PE=3 SV=1 | [more] |
| A0A6J1JV84 | 0.0 | 90.18 | Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111489180 PE=3 SV=1 | [more] |
