MC04g2000 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g2000 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 6.4e-129
Identity = 307/810 (37.90%), Postives = 433/810 (53.46%), Query Frame = 0

Query: 1    SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCE 60
            S DV ++GI+G+GGIGKTT+A+ +++ +  +F+   FL+N+RE      L HLQ +LL +
Sbjct: 214  SSDVRMVGIYGVGGIGKTTIAKVIYNELSCEFEYMSFLENIREGSNPQVLFHLQNQLLGD 273

Query: 61   VLEERRDQLIGNISR--GFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIV 120
            +LE    Q I +++     +K  +  R+V IVLDDV+D+ QLE L G R+W G GSR+I+
Sbjct: 274  ILEGEGSQNISSVAHRASMIKDILLSRRVFIVLDDVDDLSQLEYLLGHREWLGEGSRVII 333

Query: 121  TTRHRQV--VNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAG 180
            TTR++ V  V   D++YE + L+ EEA  LFS +AF  + +  S   +  L+ + V Y  
Sbjct: 334  TTRNKHVLAVQEVDDLYEVEGLNFEEACELFSLYAFK-QNLPKS--DYRNLTCRVVGYCQ 393

Query: 181  GIPLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVAC 240
            G+PLALKVLGS LC K  P WE +L KL+  P   +  VLK S++GLDR +K IFLD+AC
Sbjct: 394  GLPLALKVLGSLLCKKTIPQWEGELKKLDSEPKADIHKVLKRSYDGLDRIDKNIFLDLAC 453

Query: 241  FFKGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQED 300
            FFKG+       + D C F  E G+S L D CL+++  N +  MHDLI++MG EIVR+  
Sbjct: 454  FFKGEGRDFVLRILDGCDFPAETGISNLNDLCLITLPYNQIC-MHDLIQQMGWEIVRENF 513

Query: 301  MEEPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNF 360
              EP K SR               G  +++ + LDLSK+K V    + FA+MTKLR L  
Sbjct: 514  PVEPNKWSRLWDPCDFERALTADEGIKSVETMSLDLSKLKRVCSNSNVFAKMTKLRLLKV 573

Query: 361  FSKN-----LGESTK-------------TRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSN 420
            +S +      G+S +             ++++L          L YL WDGYP    P N
Sbjct: 574  YSSSDIDSAHGDSDEDIEEVYDVVMKDASKMQLGQSFKFPSYELRYLRWDGYPLDSLPLN 633

Query: 421  FDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGC 480
            FD  KL  L L+ S IKQL +G K    LK I+LS S  LS++ + S  P LE +  +GC
Sbjct: 634  FDGGKLVELHLKCSNIKQLWQGHKDLERLKVIDLSYSRKLSQMSEFSSMPNLERLCLSGC 693

Query: 481  IQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSI-CLKSLQELDLSGCSSLQNFPTISDY 540
            + L  IHPS+  + KL TL+LR C KL+NLP SI  L+SL+ L LS CS  + FP     
Sbjct: 694  VSLIDIHPSVGNMKKLTTLSLRSCNKLKNLPDSIGDLESLESLYLSNCSKFEKFPEKGGN 753

Query: 541  MEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNC---------------------KN-- 600
            M+ L  L L  TAI +LP SI +   L  L L+NC                     KN  
Sbjct: 754  MKSLTELDLKNTAIKDLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTA 813

Query: 601  LVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKN 660
            +  LPDSI +L+ L+ L LS C   +  PE  GN++SL  + +  T+IK +P SI  LK+
Sbjct: 814  IKDLPDSIGDLESLEILNLSDCAKFEKFPEKGGNMKSLKELDLQNTAIKDLPDSIGDLKS 873

Query: 661  IFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLV 720
            +  LS + C   E     G     L +   S++    L    +G  + L  L LS+C+  
Sbjct: 874  LKYLSLSDCSKFEKFPEKGGNMKRLLQLILSNTAIKDLP-DSIGDLESLKYLYLSDCSKF 933

Query: 721  EA-PSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCYCKKLVSLPKLPSSLSVL 740
            E  P    N+  L+ +DL     + LP SI DL  L  L L  C K    P+   ++  L
Sbjct: 934  EKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLEILNLSDCAKFEKFPEKGGNMKSL 993

BLAST of MC04g2000 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.9e-128
Identity = 299/781 (38.28%), Postives = 425/781 (54.42%), Query Frame = 0

Query: 1   SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCE 60
           S DV ++G++G+GGIGKTT+A+ +++ +  +F+   FL+N+RE     G+S LQ +LL +
Sbjct: 219 SSDVRMVGMYGVGGIGKTTIAKVIYNELSREFEYMSFLENIREKFNTQGVSPLQNQLLDD 278

Query: 61  VLEERRDQLIGNISRG--FLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIV 120
           +L+    Q I +++ G   +K  +  + V IVLDDV+D  QLE L   R+W G GSR+I+
Sbjct: 279 ILKGEGSQNINSVAHGASMIKDILSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVII 338

Query: 121 TTRHRQV--VNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAG 180
           TTR++ V  V   D++YE + L+ EEA  LFS +AF  + +  S   +  LS + V Y  
Sbjct: 339 TTRNKHVLDVQKVDDLYEVKGLNFEEACELFSLYAFE-QNLPKS--DYRNLSHRVVGYCQ 398

Query: 181 GIPLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVAC 240
           G+PLALKVLG  L  K  P WES+L KL+R P  ++ +VLK S++GL RTEK IFLDVAC
Sbjct: 399 GLPLALKVLGCLLLKKTIPEWESELRKLDREPEAEILSVLKRSYDGLGRTEKSIFLDVAC 458

Query: 241 FFKGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQED 300
           FFKG+       + DAC F  E+G+  L DKCL+++  N + +MHDLI++MG EIVR++ 
Sbjct: 459 FFKGEDRDFVSKILDACDFHAEIGIKNLNDKCLITLQYNRI-RMHDLIQQMGWEIVREKF 518

Query: 301 MEEPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNF 360
            +EP K SR               G   ++ I LDLSK+K V    +AFA+MT+LR L  
Sbjct: 519 PDEPNKWSRLWDTCDFERALTAYKGIKRVETISLDLSKLKRVCSNSNAFAKMTRLRLLKV 578

Query: 361 FSK-------------------NLGESTKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPS 420
            S                    ++     +++RL  G       L YL WDGYP  F PS
Sbjct: 579 QSSLDIDFEPEYIDADDKVELYDVVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPS 638

Query: 421 NFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAG 480
           NFD  KL  L L+ S IKQL  G K    LK I+LS S  LS++ + S  P LE +   G
Sbjct: 639 NFDGGKLVELHLKCSNIKQLRLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRG 698

Query: 481 CIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSI-CLKSLQELDLSGCSSLQNFPTISD 540
           C+ L  IHPS+  + KL TL+L+ C+KL+NLP SI  L+SL+ LDL+ CS  + FP    
Sbjct: 699 CVSLIDIHPSVGNMKKLTTLSLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGG 758

Query: 541 YMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKN---------------------- 600
            M+ L  L L  TAI +LP SI +   L  LDL++C                        
Sbjct: 759 NMKSLTELDLQNTAIKDLPDSIGDLESLKYLDLSDCSKFEKFPEKGGNMKSLRELDLRNT 818

Query: 601 -LVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLK 660
            +  LPDSI +L+ L+ L LS C   +  PE  GN++SL  + +  T+IK +P SI  L+
Sbjct: 819 AIKDLPDSIRDLESLERLYLSYCSKFEKFPEKGGNMKSLMELDLQNTAIKDLPDSIGDLE 878

Query: 661 NIFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNL 719
           ++  L  + C   E     G     L      ++    L    +G  + L SL+LS+C+ 
Sbjct: 879 SLKYLDLSNCSKFEKFPEKGGNMKSLTELFLENTAIKDLP-DSIGDLESLVSLNLSDCSK 938

BLAST of MC04g2000 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 3.6e-124
Identity = 290/759 (38.21%), Postives = 419/759 (55.20%), Query Frame = 0

Query: 4   VCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCEVLE 63
           V I+GI GM GIGKTTLA  L+ R++ QFDG CFL N+REN  + GL  L +KL   VL 
Sbjct: 209 VHIIGIVGMVGIGKTTLADCLYGRMRGQFDGSCFLTNIRENSGRSGLESLLQKLFSTVLN 268

Query: 64  ERRDQL--IGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTTR 123
           +R  ++   GN    F ++R++ +++LIVLDDVND +Q+  L G   W+  GSRII+TTR
Sbjct: 269 DRDLEIGAPGNAHERF-ERRLKSKRLLIVLDDVNDEKQIRYLMGHCKWYQGGSRIIITTR 328

Query: 124 HRQVVNG-ADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPL 183
             +++       Y    L+  EA++LFS +AF+        EG   L+   ++YA G PL
Sbjct: 329 DSKLIETIKGRKYVLPKLNDREALKLFSLNAFSNSFPLKEFEG---LTNMVLDYAKGHPL 388

Query: 184 ALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKG 243
           ALKVLGS LC +D   WE+KL +L+   +  +  VL+ S+E L   +K +FLD+ACFF+ 
Sbjct: 389 ALKVLGSDLCERDDLYWEAKLDRLKSRSHGDIYEVLETSYEELTTEQKNVFLDIACFFRS 448

Query: 244 DHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEI--------- 303
           ++V    ++ ++ G      +  L+DKCL++++ N + +MHD+++ M KEI         
Sbjct: 449 ENVDYVTSLLNSHGVDVSGVVKDLVDKCLITLSDNRI-EMHDMLQTMAKEISLKVETIGI 508

Query: 304 ----------------VR---QEDMEEPGKRSRGTNAIKGILLDLSKIKEVELGEDAFAE 363
                           +R    ED+ +      GT+ I+GI LD SK++ + L   AF  
Sbjct: 509 RDCRWLSRHGNQCQWHIRLWDSEDICDLLTEGLGTDKIRGIFLDTSKLRAMRLSAKAFQG 568

Query: 364 MTKLRFLNFFSKNL--GESTKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLF 423
           M  L++L  +  +   G   + ++ L  GL+ LP+ L YL W GYP +  P +FDP  L 
Sbjct: 569 MYNLKYLKIYDSHCSRGCEAEFKLHLRRGLSFLPNELTYLHWHGYPLQSIPLDFDPKNLV 628

Query: 424 WLRLRHSCIKQLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIH 483
            L+L HS ++++   +K    LK ++LS S  L +   ++ A  LE +N  GC  L  + 
Sbjct: 629 DLKLPHSQLEEIWDDEKDVGMLKWVDLSHSINLRQCLGLANAHNLERLNLEGCTSLKKLP 688

Query: 484 PSMATLSKLKTLNLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLS 543
            ++  L KL  LNLRDC  LR+LP  I  +SLQ L LSGCSSL+ FP IS+ +E    L 
Sbjct: 689 STINCLEKLIYLNLRDCTSLRSLPKGIKTQSLQTLILSGCSSLKKFPLISENVE---VLL 748

Query: 544 LSGTAISNLPSSIENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPEN 603
           L GT I +LP SI+ F  L  L+L NCK L  L   +  LKCL  LILS C  L+  PE 
Sbjct: 749 LDGTVIKSLPESIQTFRRLALLNLKNCKKLKHLSSDLYKLKCLQELILSGCSQLEVFPEI 808

Query: 604 IGNLESLSTVYVDETSIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSS 663
             ++ESL  + +D+TSI ++P  ++ L NI   S  G     T S +    +F       
Sbjct: 809 KEDMESLEILLMDDTSITEMP-KMMHLSNIKTFSLCG-----TSSHVSVSMFF------- 868

Query: 664 SSCNMGLQLPPLGGFKQLSSLSLSNCNLVEAPSGLHNLPCLSSIDLSENNFESLPQSIYD 723
                   +PP  G  +L+ L LS C+L + P  +  L  L S+ LS NN E+LP+S   
Sbjct: 869 --------MPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQSLCLSGNNIENLPESFNQ 928

Query: 724 LPELGTLMLCYCKKLVSLPKLPSSLSVLLADECTSLESI 730
           L  L    L +CK L SLP LP +L  L A EC SLE++
Sbjct: 929 LNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETL 938

BLAST of MC04g2000 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 1.9e-117
Identity = 276/744 (37.10%), Postives = 403/744 (54.17%), Query Frame = 0

Query: 4   VCILGIWGMGGIGKTTLARALFDRI------KSQFDGCCFLKNVRENQIKHGLSHLQEKL 63
           V I+GIWGMGG+GKTT+ARA+FD +        QFDG CFLK+++EN  K G+  LQ  L
Sbjct: 209 VRIMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGACFLKDIKEN--KRGMHSLQNAL 268

Query: 64  LCEVLEERRDQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQ-LEDLAGGRDWFGPGSRI 123
           L E+L E+ +       +  +  R+R +KVLIVLDD+++ +  LE LAG  DWFG GSRI
Sbjct: 269 LSELLREKANYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRI 328

Query: 124 IVTTRHRQVVNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAG 183
           I+TTR + ++   D +YE   L   E+++LF  HAF   G +   E F +LS + VNYA 
Sbjct: 329 IITTRDKHLIEKNDIIYEVTALPDHESIQLFKQHAF---GKEVPNENFEKLSLEVVNYAK 388

Query: 184 GIPLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVAC 243
           G+PLALKV GS L       W+S +  ++      + + LK+S++GL+  ++ +FLD+AC
Sbjct: 389 GLPLALKVWGSLLHNLRLTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPKQQEMFLDIAC 448

Query: 244 FFKGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQED 303
           F +G+       + ++C    E GL +LIDK L+ I+  +  QMHDLI++MGK IV  + 
Sbjct: 449 FLRGEEKDYILQILESCHIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMGKYIVNFQ- 508

Query: 304 MEEPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNF 363
            ++PG+RSR               GT A++ I +  S    +     A   M +LR    
Sbjct: 509 -KDPGERSRLWLAKEVEEVMSNNTGTMAMEAIWVS-SYSSTLRFSNQAVKNMKRLRVF-- 568

Query: 364 FSKNLGESTKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQ 423
              N+G S+       + +  LP++L       YP++ FPS F+   L  L+LRH+ ++ 
Sbjct: 569 ---NMGRSS-----THYAIDYLPNNLRCFVCTNYPWESFPSTFELKMLVHLQLRHNSLRH 628

Query: 424 LSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKT 483
           L    K   +L+ I+LS S+ L+  PD +  P LE +N   C  L  +H S+   SK+  
Sbjct: 629 LWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEVHHSLGCCSKVIG 688

Query: 484 LNLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPS 543
           L L DC+ L+  P  + ++SL+ L L  C SL+  P I   M+    + + G+ I  LPS
Sbjct: 689 LYLNDCKSLKRFP-CVNVESLEYLGLRSCDSLEKLPEIYGRMKPEIQIHMQGSGIRELPS 748

Query: 544 SIENF-YGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTV 603
           SI  +   +  L L N KNLV+LP SIC LK L SL +S C  L+ LPE IG+L++L   
Sbjct: 749 SIFQYKTHVTKLLLWNMKNLVALPSSICRLKSLVSLSVSGCSKLESLPEEIGDLDNLRVF 808

Query: 604 YVDETSIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLP 663
              +T I + PSSI+ L  +  L F G +                         +  + P
Sbjct: 809 DASDTLILRPPSSIIRLNKLIILMFRGFKD-----------------------GVHFEFP 868

Query: 664 PLG-GFKQLSSLSLSNCNLVEA--PSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTL 722
           P+  G   L  L+LS CNL++   P  + +L  L  +DLS NNFE LP SI  L  L +L
Sbjct: 869 PVAEGLHSLEYLNLSYCNLIDGGLPEEIGSLSSLKKLDLSRNNFEHLPSSIAQLGALQSL 910

BLAST of MC04g2000 vs. ExPASy Swiss-Prot
Match: F4JT80 (Disease resistance protein RPP2B OS=Arabidopsis thaliana OX=3702 GN=RPP2B PE=1 SV=2)

HSP 1 Score: 414.1 bits (1063), Expect = 3.4e-114
Identity = 267/744 (35.89%), Postives = 416/744 (55.91%), Query Frame = 0

Query: 4   VCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCEVLE 63
           V ++G+ GM GIGKTT+A  ++ +   +FDG  FL+++ +N  ++GL +L +KLL ++L+
Sbjct: 214 VRVVGVLGMTGIGKTTVADIVYKQNFQRFDGYEFLEDIEDNSKRYGLPYLYQKLLHKLLD 273

Query: 64  ERRDQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTTRHR 123
               + +   ++G  +  +R +K+ IVLD+V + +Q+E L G ++ +  GSRI++ TR +
Sbjct: 274 ---GENVDVRAQGRPENFLRNKKLFIVLDNVTEEKQIEYLIGKKNVYRQGSRIVIITRDK 333

Query: 124 QVV-NGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPLAL 183
           +++   AD  Y    L+  EAM LF    F   G  +  E F++LS   V YA G+PLAL
Sbjct: 334 KLLQKNADATYVVPRLNDREAMELFCLQVF---GNHYPTEEFVDLSNDFVCYAKGLPLAL 393

Query: 184 KVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKGDH 243
           K+LG  L   D   W+ KL  L+  P++++Q  LK S++ LD  +K +FLD+ACFF+ + 
Sbjct: 394 KLLGKGLLTHDINYWKKKLEFLQVNPDKELQKELKSSYKALDDDQKSVFLDIACFFRSEK 453

Query: 244 VGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEPGK 303
                ++  +     +  +  L +KCL++I+ + + +MHDL+  MGKEI +++ + + G+
Sbjct: 454 ADFVSSILKSDDIDAKDVMRELEEKCLVTISYDRI-EMHDLLHAMGKEIGKEKSIRKAGE 513

Query: 304 RSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLG 363
           R R               GT  ++GI L++S+++ ++L   AF  ++KL+FL F S +  
Sbjct: 514 RRRLWNHKDIRDILEHNTGTECVRGIFLNMSEVRRIKLFPAAFTMLSKLKFLKFHSSHCS 573

Query: 364 E--STKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKG 423
           +        +        PD L+YL W GYPY   PS+FDP +L  L LR+S IKQL + 
Sbjct: 574 QWCDNDHIFQCSKVPDHFPDELVYLHWQGYPYDCLPSDFDPKELVDLSLRYSHIKQLWED 633

Query: 424 QKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLR 483
           +K   +L+ ++L  S+ L  +  +S A  LE ++  GC  L  +  S+  +++L  LNLR
Sbjct: 634 EKNTESLRWVDLGQSKDLLNLSGLSRAKNLERLDLEGCTSL-DLLGSVKQMNELIYLNLR 693

Query: 484 DCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIEN 543
           DC  L +LP    +KSL+ L LSGC  L++F  IS+ +E   SL L GTAI  +   IE+
Sbjct: 694 DCTSLESLPKGFKIKSLKTLILSGCLKLKDFHIISESIE---SLHLEGTAIERVVEHIES 753

Query: 544 FYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDET 603
            + L+ L+L NC+ L  LP+ +  LK L  L+LS C  L+ LP     +E L  + +D T
Sbjct: 754 LHSLILLNLKNCEKLKYLPNDLYKLKSLQELVLSGCSALESLPPIKEKMECLEILLMDGT 813

Query: 604 SIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGF 663
           SIKQ P  + CL N+   SF  CR     S+                   GL + P  G 
Sbjct: 814 SIKQTP-EMSCLSNLKICSF--CRPVIDDST-------------------GLVVLPFSGN 873

Query: 664 KQLSSLSLSNCNLVEAPSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCYCKKL 723
             LS L L+NCN+ + P    +L  L  + LS NN E+LP+SI  L  L  L L +C +L
Sbjct: 874 SFLSDLYLTNCNIDKLPDKFSSLRSLRCLCLSRNNIETLPESIEKLYSLLLLDLKHCCRL 924

Query: 724 VSLPKLPSSLSVLLADECTSLESI 730
            SLP LPS+L  L A  C SLE++
Sbjct: 934 KSLPLLPSNLQYLDAHGCGSLENV 924

BLAST of MC04g2000 vs. NCBI nr
Match: XP_022152475.1 (TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 1496 bits (3874), Expect = 0.0
Identity = 743/743 (100.00%), Postives = 743/743 (100.00%), Query Frame = 0

Query: 1   SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCE 60
           SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCE
Sbjct: 74  SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCE 133

Query: 61  VLEERRDQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTT 120
           VLEERRDQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTT
Sbjct: 134 VLEERRDQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTT 193

Query: 121 RHRQVVNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPL 180
           RHRQVVNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPL
Sbjct: 194 RHRQVVNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPL 253

Query: 181 ALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKG 240
           ALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKG
Sbjct: 254 ALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKG 313

Query: 241 DHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEP 300
           DHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEP
Sbjct: 314 DHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEP 373

Query: 301 GKRSRGTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLGESTKTRIRLPHGL 360
           GKRSRGTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLGESTKTRIRLPHGL
Sbjct: 374 GKRSRGTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLGESTKTRIRLPHGL 433

Query: 361 TMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDS 420
           TMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDS
Sbjct: 434 TMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDS 493

Query: 421 EYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSICLK 480
           EYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSICLK
Sbjct: 494 EYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSICLK 553

Query: 481 SLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKNL 540
           SLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKNL
Sbjct: 554 SLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKNL 613

Query: 541 VSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKNI 600
           VSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKNI
Sbjct: 614 VSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKNI 673

Query: 601 FELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLVE 660
           FELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLVE
Sbjct: 674 FELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLVE 733

Query: 661 APSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCYCKKLVSLPKLPSSLSVLLA 720
           APSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCYCKKLVSLPKLPSSLSVLLA
Sbjct: 734 APSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCYCKKLVSLPKLPSSLSVLLA 793

Query: 721 DECTSLESIMSFEFFYKSDNRTY 743
           DECTSLESIMSFEFFYKSDNRTY
Sbjct: 794 DECTSLESIMSFEFFYKSDNRTY 816

BLAST of MC04g2000 vs. NCBI nr
Match: XP_024047583.1 (disease resistance-like protein DSC1 [Citrus clementina])

HSP 1 Score: 598 bits (1543), Expect = 4.81e-196
Identity = 335/749 (44.73%), Postives = 462/749 (61.68%), Query Frame = 0

Query: 1   SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHG-LSHLQEKLLC 60
           S++V  LGIWG+GGIGKTT+A A+F +I + FDG  F++NVRE   K G L+HL++ LL 
Sbjct: 227 SKEVYSLGIWGIGGIGKTTIASAIFSKISTHFDGSYFIQNVREESEKSGGLAHLRQTLLS 286

Query: 61  EVLEERRDQLI-GNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIV 120
            +LE+    ++  NI   F  KR+  +KVLIV DDV+  EQ+E L G + W  PGSR+++
Sbjct: 287 AILEDGNVSIVCRNIGLNFRSKRLSRKKVLIVFDDVSTSEQMEFLIGDKGWLMPGSRLVI 346

Query: 121 TTRHRQVVN--GADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAG 180
           T R +QV+   G D +YE + L  ++A+ LFS +AF   G +H    ++ELS K + YA 
Sbjct: 347 TARDKQVLRNCGVDTIYEVKELFDDDALMLFSRYAF---GKNHPNADYMELSNKIIKYAK 406

Query: 181 GIPLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVAC 240
           G+PLA+KV G  LCG+    WES + K++R+P+  +Q VLKVS++GLD  E+ IFLD+AC
Sbjct: 407 GVPLAIKVFGRFLCGRRIQDWESAMNKMKRIPHVDIQRVLKVSYDGLDDEEQNIFLDIAC 466

Query: 241 FFKGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQED 300
           FFKG+         D CGF  E+G+SVL+DKCL+ I+ N++  MHDL++EMG+EIVRQE 
Sbjct: 467 FFKGEDKDCVIRFLDGCGFSSEIGISVLVDKCLMIISNNTIM-MHDLLQEMGREIVRQES 526

Query: 301 MEEPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNF 360
           +++PGKRSR               GT +I+GI LD+S+++++ L   +F  M KLRF   
Sbjct: 527 IKDPGKRSRLWHHEDIYNVLTKNMGTESIEGISLDMSEVEDIHLNARSFTNMHKLRFFKL 586

Query: 361 FSKNLGESTKTRIRLPHGLTMLPDS-LMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIK 420
           +S + GE+    +   H    L  + L YL W G P K   S   P+ L  L + HS IK
Sbjct: 587 YSSHYGEN----VNKVHNFRGLESTELRYLQWHGCPLKSLSSKIHPENLVSLEMPHSSIK 646

Query: 421 QLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLK 480
           QL KG +   NLKH+NLS SE+L+++PD+S A  LE +NF GC  L   H S+  L+KL 
Sbjct: 647 QLWKGVQRLVNLKHLNLSHSEHLTKIPDLSLATNLESLNFQGCTSLLETHSSIQYLNKLI 706

Query: 481 TLNLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLP 540
            LNL  CR L +L +S  L+SL++L LSGCS+L +FP +S  +E L   SL GTAI  LP
Sbjct: 707 VLNLGHCRSLTSLSTSTHLESLKKLILSGCSNLMSFPELSCNIEEL---SLDGTAIQELP 766

Query: 541 SSIENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTV 600
           SSIE    L+ L+L NC  L  LP  IC LK L+ L LS C NLQ LP  +GNLE+L  +
Sbjct: 767 SSIERLSSLILLNLGNCSRLEGLPSKICKLKSLERLNLSGCSNLQRLPNELGNLEALKEL 826

Query: 601 YVDETSIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLP 660
             +  +I++VPSSIVCLKN+  LSF   +G E                      MGL LP
Sbjct: 827 KAEGIAIREVPSSIVCLKNLGRLSFESFKGHE---------------------QMGLLLP 886

Query: 661 PLGGFKQLSSLSLSNCNLVEAPSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLC 720
              G   L+ L L++C++ E P  L  L  L  + L +NNFE +P+SI  L  L  L + 
Sbjct: 887 ISFGLTSLTYLRLTDCSITELPECLGQLSSLRILFLDKNNFERIPESIICLSHLYWLRIS 943

Query: 721 YCKKLVSLPKLPSSLSVLLADECTSLESI 729
           YC++L SLP+LP  LS + A  C+SLE++
Sbjct: 947 YCERLKSLPELPCDLSDIEAHCCSSLEAL 943

BLAST of MC04g2000 vs. NCBI nr
Match: XP_006470767.1 (disease resistance-like protein DSC1 [Citrus sinensis])

HSP 1 Score: 598 bits (1541), Expect = 6.28e-196
Identity = 335/749 (44.73%), Postives = 462/749 (61.68%), Query Frame = 0

Query: 1   SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHG-LSHLQEKLLC 60
           S++V  LGIWG+GGIGKTT+A A+F +I + FDG  F++NVRE   K G L+HL++ LL 
Sbjct: 211 SKEVYSLGIWGIGGIGKTTIASAIFSKISTDFDGSYFIQNVREESEKSGGLAHLRQTLLS 270

Query: 61  EVLEERRDQLI-GNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIV 120
            +LE+    ++  NI   F  KR+  +KVLIV DDV+  EQ+E L G + W  PGSR+++
Sbjct: 271 AILEDGNVSIVCRNIGLNFRSKRLSRKKVLIVFDDVSTSEQMEFLIGDKGWLMPGSRLVI 330

Query: 121 TTRHRQVVN--GADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAG 180
           T R +QV+   G D +YE + L  ++A+ LFS +AF   G +H    ++ELS K + YA 
Sbjct: 331 TARDKQVLRNCGVDTIYEVKELFDDDALMLFSRYAF---GKNHPNADYMELSNKIIKYAK 390

Query: 181 GIPLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVAC 240
           G+PLA+KVLG  LCG+    WES + K++R+P+  +Q VLKVS++GLD  E+ IFLD+AC
Sbjct: 391 GVPLAIKVLGRFLCGRRIQDWESAMNKMKRIPHVDIQRVLKVSYDGLDDEEQNIFLDIAC 450

Query: 241 FFKGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQED 300
           FFKG+         D CGF  E+G+SVL+DKCL+ I+ N++  MHDL++EMG+EIVRQE 
Sbjct: 451 FFKGEDKDCVIRFLDGCGFSAEIGISVLVDKCLMIISNNTIM-MHDLLQEMGREIVRQES 510

Query: 301 MEEPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNF 360
           +++PGKRSR               GT +I+GI LD+S+++++ L   +F  M KLRF   
Sbjct: 511 IKDPGKRSRLWHHEDIYNVLTKNMGTESIEGISLDMSEVEDIHLNARSFTNMHKLRFFKL 570

Query: 361 FSKNLGESTKTRIRLPHGLTMLPDS-LMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIK 420
           +S + GE+    +   H    L  + L YL W G P K   S   P+ L  L + HS IK
Sbjct: 571 YSSHYGEN----VNKVHNFRGLESTELRYLQWHGCPLKSLSSKIHPENLVSLEMPHSSIK 630

Query: 421 QLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLK 480
           QL KG +   NLKH+NLS SE+L+++PD+S A  LE +N  GC  L   H S+  L+KL 
Sbjct: 631 QLWKGVQRLVNLKHLNLSHSEHLTKIPDLSLATNLESLNLQGCTSLLETHSSIQYLNKLI 690

Query: 481 TLNLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLP 540
            LNL  CR L +L +S  L+SL++L LSGCS+L +FP +S  +E L   SL GTAI  LP
Sbjct: 691 VLNLGHCRSLTSLSTSTHLESLKKLILSGCSNLMSFPELSCNIEEL---SLDGTAIQELP 750

Query: 541 SSIENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTV 600
           SSIE    L+ L+L NC  L  LP  IC LK L+ L LS C NLQ LP  +GNLE+L  +
Sbjct: 751 SSIERLSSLILLNLGNCSRLEGLPSKICKLKSLERLNLSGCSNLQRLPNELGNLEALKEL 810

Query: 601 YVDETSIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLP 660
             +  +I++VPSSIVCLKN+  LSF   +G E                      MGL LP
Sbjct: 811 KAEGIAIREVPSSIVCLKNLGRLSFESFKGHE---------------------QMGLLLP 870

Query: 661 PLGGFKQLSSLSLSNCNLVEAPSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLC 720
              G   L+ L L++C++ E P  L  L  L  + L +NNFE +P+SI  L  L  L + 
Sbjct: 871 ISFGLTSLTYLRLTDCSITELPECLGQLSSLRILFLDKNNFERIPESIICLSHLYWLRIS 927

Query: 721 YCKKLVSLPKLPSSLSVLLADECTSLESI 729
           YC++L SLP+LP  LS + A  C+SLE++
Sbjct: 931 YCERLKSLPELPCDLSDIEAHCCSSLEAL 927

BLAST of MC04g2000 vs. NCBI nr
Match: XP_034680044.1 (disease resistance protein RUN1-like [Vitis riparia])

HSP 1 Score: 583 bits (1503), Expect = 5.08e-190
Identity = 351/772 (45.47%), Postives = 470/772 (60.88%), Query Frame = 0

Query: 1   SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCE 60
           S DV ++G+WGM GIGKTT+A  + +RI +QF+GCCFL NVRE   K+    LQ +LL +
Sbjct: 212 SIDVRMVGLWGMPGIGKTTIAEVVCERICNQFEGCCFLSNVREKSQKNDSIDLQIELLSQ 271

Query: 61  VLEERR-DQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVT 120
           VL E   +  + N     +K R+R  K LI+LDDV+  +QLEDLA   +WFGPGSRII+T
Sbjct: 272 VLREGNLNTKVFNRGMNIIKDRLRSMKALIILDDVDHQQQLEDLARNHNWFGPGSRIIIT 331

Query: 121 TRHRQVVNG-ADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGI 180
           +R +QV+N   D +YE + L ++EA +LF  +AF  +      E FL L   A+ Y  G+
Sbjct: 332 SRDKQVLNRQVDAIYEVRELKYDEARKLFCQYAFRQK---FPTEDFLTLCGDALYYTKGV 391

Query: 181 PLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFF 240
           PLALKVLGS L  K  P W+S+L KL++ PN+KVQNVLK SF+GLD  EK IFLDVA F+
Sbjct: 392 PLALKVLGSFLYEKSVPEWKSELDKLKQFPNKKVQNVLKTSFDGLDDNEKDIFLDVAFFY 451

Query: 241 KGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDME 300
           KG      + + D+C FF + G+  L+DK L++I++N +  MHDL++EMG E+VRQE ++
Sbjct: 452 KGQDKDFVREILDSCFFF-DTGIRSLLDKSLITISKNKLC-MHDLLQEMGWEVVRQESIK 511

Query: 301 EPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNF-- 360
           + G RSR               GT A++GI+LDLS  KE+ L  DAFA+M +LR L F  
Sbjct: 512 DLGTRSRLLVHDDVNHVLTTNTGTQAVEGIVLDLSASKELNLNVDAFAKMNRLRLLRFCN 571

Query: 361 ---FSKNLGESTKTRI-------RLPHG-----------LTMLPDSLMYLSWDGYPYKFF 420
              F  +   S K  I       R  HG              L ++L  L W GYP K  
Sbjct: 572 CEFFGSSEYLSRKELIARADAWRRKYHGDNGCKLHLFGNFKFLSNNLRSLHWHGYPLKTL 631

Query: 421 PSNFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINF 480
           P+NF P+KL  L +  S + QL +G K F  LK I L  S+ L+  PD S  P L  +  
Sbjct: 632 PANFYPEKLVELNMCSSQLAQLWEGNKPFEKLKFIKLCHSQLLTNTPDFSGTPKLRRLIL 691

Query: 481 AGCIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTIS 540
            GC  L  +HPS+  L +L  LNL  C+KL + PSSI ++SLQ L LSGCS L+NFP + 
Sbjct: 692 KGCTSLVKVHPSIGALKELIFLNLEGCKKLSSFPSSIHMESLQILVLSGCSKLKNFPEVQ 751

Query: 541 DYMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSS 600
             ME+L  L L GTAI  LPSSI +  GLV LDLTNCK L +LP SIC L  L +L LS 
Sbjct: 752 GQMENLSELYLDGTAIRGLPSSIAHLNGLVLLDLTNCKQLANLPQSICELTSLQTLTLSG 811

Query: 601 CENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKNIFELSFNGCRGTETLS-SLGF 660
           C  L+ LP+++G+L+ L  +  + T+I++VP  I  L N+  LSF GC+G +++S +L F
Sbjct: 812 CIELKKLPDDLGSLQCLVELNANGTAIQEVPPCITLLSNLRVLSFAGCKGGDSMSWNLVF 871

Query: 661 LSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLVEA--PSGLHNLPCLSSIDLS 720
             W        SS    L+LP L G   L SL+LS+CNLVE   PS L +L  L  +DLS
Sbjct: 872 SFW--------SSPTKYLRLPSLLGLHSLQSLNLSDCNLVEGALPSDLASLSSLKDLDLS 931

Query: 721 ENNFE-SLPQSIYDLPELGTLMLCYCKKLVSLPKLPSSLSVLLADECTSLES 728
            N+   +LP S+  L +L  L+L +CK L SLP+LPSS+  L  ++CTSLE+
Sbjct: 932 RNSLIITLPDSLSQLSQLKKLILKHCKSLQSLPELPSSIETLECNDCTSLET 970

BLAST of MC04g2000 vs. NCBI nr
Match: XP_034674930.1 (LOW QUALITY PROTEIN: disease resistance protein RUN1-like [Vitis riparia])

HSP 1 Score: 583 bits (1502), Expect = 7.15e-190
Identity = 350/772 (45.34%), Postives = 470/772 (60.88%), Query Frame = 0

Query: 1   SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCE 60
           S DV ++G+WGM GIGKTT+A  + +RI +QF+GCCFL NVRE   K+    LQ +LL +
Sbjct: 212 SIDVRMVGLWGMPGIGKTTIAEVVCERICNQFEGCCFLSNVREKSQKNDSIDLQIELLSQ 271

Query: 61  VLEERR-DQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVT 120
           VL E   +  I N     +K R+   K LI+LDDV+  +QLEDLAG  +WFGPGSRII+T
Sbjct: 272 VLREGNLNTKIFNRGMNIIKDRLHSMKALIILDDVDHQQQLEDLAGNHNWFGPGSRIIIT 331

Query: 121 TRHRQVVNG-ADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGI 180
           +R +QV+N   D +YE + L ++EA +LF  +AF  +      E F+ L   A+ Y  G+
Sbjct: 332 SRDKQVLNPQVDAIYEVRELKYDEARKLFCQYAFRQK---FPTEDFMTLCGDALYYTKGV 391

Query: 181 PLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFF 240
           PLALKVLGS L  K  P W+S+L KL++ PN+KVQNVLK SF+GLD  EK IFLDVA F+
Sbjct: 392 PLALKVLGSFLYEKSVPEWKSELDKLKQFPNKKVQNVLKTSFDGLDDNEKDIFLDVAFFY 451

Query: 241 KGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDME 300
           KG      + + D+C FF + G+  L+DK L++I++N +  MHDL++EMG E+VRQE ++
Sbjct: 452 KGQDKDFVREILDSCFFF-DTGIRSLLDKSLITISKNKLC-MHDLLQEMGWEVVRQESIK 511

Query: 301 EPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNF-- 360
           + G RSR               GT A++GI+LDLS  KE+ L  DAFA+M +LR L F  
Sbjct: 512 DLGTRSRLLVHDDVNHVLTTNTGTQAVEGIVLDLSASKELNLNVDAFAKMNRLRLLRFCN 571

Query: 361 ---FSKNLGESTKTRI-------RLPHG-----------LTMLPDSLMYLSWDGYPYKFF 420
              F  +   S K  I       R  HG              L ++L  L W GYP K  
Sbjct: 572 CEFFGSSEYLSRKELIARADAWRRKYHGDNGCKLHLFGNFKFLSNNLRSLHWHGYPLKTL 631

Query: 421 PSNFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINF 480
           P+NF P+KL  L +  S + QL +G K F  LK I L  S+ L+  PD S  P L  +  
Sbjct: 632 PANFYPEKLVELNMCSSQLAQLWEGNKPFEKLKFIKLCHSQLLTNTPDFSGTPKLRRLIL 691

Query: 481 AGCIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTIS 540
            GC  L  +HPS+  L +L  LNL  C+KL + PSSI ++SLQ L LSGCS L+NFP + 
Sbjct: 692 KGCTSLVKVHPSIGALKELIFLNLEGCKKLSSFPSSIHMESLQILILSGCSKLKNFPEVQ 751

Query: 541 DYMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSS 600
             ME+L  L L GTAI  LPSSI +  GLV LDLTNCK L +LP SIC L  L +L LS 
Sbjct: 752 GQMENLSELYLDGTAIRGLPSSIAHLNGLVLLDLTNCKQLANLPQSICELTSLQTLTLSG 811

Query: 601 CENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKNIFELSFNGCRGTETLS-SLGF 660
           C  L+ LP+++G+L+ L  +  + T+I++VP  I  L N+  LSF GC+G +++S +L F
Sbjct: 812 CIELKKLPDDLGSLQCLVELNANGTAIQEVPPCITLLSNLRVLSFAGCKGGDSMSRNLVF 871

Query: 661 LSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLVEA--PSGLHNLPCLSSIDLS 720
             W        SS    L+LP L G   L SL+LS+CNLVE   PS L +L  L  +DLS
Sbjct: 872 SFW--------SSPTKYLRLPSLLGLHSLQSLNLSDCNLVEGALPSDLASLSSLKDLDLS 931

Query: 721 ENNFE-SLPQSIYDLPELGTLMLCYCKKLVSLPKLPSSLSVLLADECTSLES 728
            N+   ++P S+  L +L  L+L +CK L SLP+LPSS+  L  ++CTSLE+
Sbjct: 932 RNSLIITVPDSLSQLSQLKKLILKHCKSLQSLPELPSSIETLECNDCTSLET 970

BLAST of MC04g2000 vs. ExPASy TrEMBL
Match: A0A6J1DE10 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111020196 PE=4 SV=1)

HSP 1 Score: 1496 bits (3874), Expect = 0.0
Identity = 743/743 (100.00%), Postives = 743/743 (100.00%), Query Frame = 0

Query: 1   SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCE 60
           SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCE
Sbjct: 74  SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCE 133

Query: 61  VLEERRDQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTT 120
           VLEERRDQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTT
Sbjct: 134 VLEERRDQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTT 193

Query: 121 RHRQVVNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPL 180
           RHRQVVNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPL
Sbjct: 194 RHRQVVNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPL 253

Query: 181 ALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKG 240
           ALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKG
Sbjct: 254 ALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKG 313

Query: 241 DHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEP 300
           DHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEP
Sbjct: 314 DHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEP 373

Query: 301 GKRSRGTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLGESTKTRIRLPHGL 360
           GKRSRGTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLGESTKTRIRLPHGL
Sbjct: 374 GKRSRGTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLGESTKTRIRLPHGL 433

Query: 361 TMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDS 420
           TMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDS
Sbjct: 434 TMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDS 493

Query: 421 EYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSICLK 480
           EYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSICLK
Sbjct: 494 EYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSICLK 553

Query: 481 SLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKNL 540
           SLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKNL
Sbjct: 554 SLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKNL 613

Query: 541 VSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKNI 600
           VSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKNI
Sbjct: 614 VSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKNI 673

Query: 601 FELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLVE 660
           FELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLVE
Sbjct: 674 FELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLVE 733

Query: 661 APSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCYCKKLVSLPKLPSSLSVLLA 720
           APSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCYCKKLVSLPKLPSSLSVLLA
Sbjct: 734 APSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCYCKKLVSLPKLPSSLSVLLA 793

Query: 721 DECTSLESIMSFEFFYKSDNRTY 743
           DECTSLESIMSFEFFYKSDNRTY
Sbjct: 794 DECTSLESIMSFEFFYKSDNRTY 816

BLAST of MC04g2000 vs. ExPASy TrEMBL
Match: A0A5J5B3B3 (TIR domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_029528 PE=4 SV=1)

HSP 1 Score: 583 bits (1502), Expect = 5.32e-190
Identity = 340/757 (44.91%), Postives = 462/757 (61.03%), Query Frame = 0

Query: 3   DVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCEVL 62
           D+ ++GI GMGGIGKTT+A+A++D +  QF+G  +L NVRE   KHGL  LQE+LL EVL
Sbjct: 214 DIRMVGICGMGGIGKTTIAKAVYDLLSCQFEGSSYLANVREVSRKHGLELLQEQLLNEVL 273

Query: 63  EERRDQLIGNISRG--FLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTT 122
            E  D  I N+ +G   +K R+   +VL+VLDDV+  EQLE LA   DWFG GSRII+TT
Sbjct: 274 MES-DIKIRNVDKGTNMIKNRLPCMRVLVVLDDVDRSEQLEALAKKHDWFGLGSRIIITT 333

Query: 123 RHRQVVNG--ADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGI 182
           R   +++    + VYEA+ L++EEA++LFS  AF     +   EG+ EL  K V     +
Sbjct: 334 RDVHLLDRYEVNYVYEAKELNYEEAVKLFSCKAFKR---NQPTEGYEELIEKVVYCTKRV 393

Query: 183 PLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFF 242
           PLALK+LGS L G+    WES L +L+  PN +V  +LK+S++GL+ TEK +FLD+ACFF
Sbjct: 394 PLALKLLGSFLSGRSINEWESALNRLKESPNPQVLEILKISYDGLEYTEKELFLDIACFF 453

Query: 243 KGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDME 302
           KG++      + D C  +P +G+S+L+ K L++I++N +  MHDLI+EMG  IVRQE  +
Sbjct: 454 KGENKDYVAKLLDGCDLYPVIGISILVQKSLITISKNELL-MHDLIQEMGWHIVRQESPK 513

Query: 303 EPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFS 362
           EPGKR+R               GT  ++GI LD    KE+ L  +AF++M KLR L    
Sbjct: 514 EPGKRTRLWLHKDIYHVLTENTGTETVEGINLDSRGTKELHLSAEAFSKMKKLRLLKI-- 573

Query: 363 KNLGESTKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLS 422
                   + ++LPHGL  L + L  L W GYP K  P +F P+KL  L+L +S I+QL 
Sbjct: 574 --------SNVQLPHGLEYLSNELRLLDWHGYPLKHMPFSFQPEKLVELKLCYSHIEQLW 633

Query: 423 KGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLN 482
            G      LK I+LS S  L++ PD +    LE +   GC  L  +HPS+  +  L  LN
Sbjct: 634 NGVMQLDKLKLIDLSHSRQLTKTPDFTGVRNLEKLVLEGCTSLLEVHPSIGDIRGLMCLN 693

Query: 483 LRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSI 542
           L+DC+ L+ LPSS  L++L+ L LSGCS L+N   I + M+ L  L L GT +  +PSS+
Sbjct: 694 LKDCKNLKILPSSNSLENLRILVLSGCSQLKNISKIFENMKLLSELYLDGTNVKEVPSSV 753

Query: 543 ENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVD 602
           E+   LV + L +CK L SLP  IC LKCL  + LS C  L  LP+N+G +E L  + V+
Sbjct: 754 EHLSSLVLISLRDCKKLTSLPSIICRLKCLKKINLSGCSKLDKLPDNLGEMECLEKLSVN 813

Query: 603 ETSIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLG 662
            T+IKQ P SIV LKN+ +LSF GC+G  +     F S   PR    S   +GL LP L 
Sbjct: 814 GTAIKQPPPSIVLLKNLKKLSFRGCKGVTSKPWSLFPSLLFPRKSQDS---IGLILPSLS 873

Query: 663 GFKQLSSLSLSNCNLVEA--PSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCY 722
           G   L  L L++CNL E   P  L  LP L  ++LS NNF+SLP S+  L  L  L L  
Sbjct: 874 GLSSLKELDLTDCNLFEGTIPCDLDCLPSLVYLNLSRNNFDSLPASLVGLSRLRLLELVG 933

Query: 723 CKKLVSLPKLPSSLSVLLADECTSLESIMSFEFFYKS 738
           CK+L SLP+LPSS++V+ AD+CTSLE +      YKS
Sbjct: 934 CKRLKSLPELPSSIAVINADDCTSLERLSIPSTMYKS 952

BLAST of MC04g2000 vs. ExPASy TrEMBL
Match: V4U0N7 (TIR domain-containing protein OS=Citrus clementina OX=85681 GN=CICLE_v10014074mg PE=4 SV=1)

HSP 1 Score: 582 bits (1499), Expect = 7.88e-190
Identity = 329/749 (43.93%), Postives = 455/749 (60.75%), Query Frame = 0

Query: 1   SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHG-LSHLQEKLLC 60
           S++V  LGIWG+GGIGKTT+A A+F +I + FDG  F++NVRE   K G L+HL++ LL 
Sbjct: 211 SKEVYSLGIWGIGGIGKTTIASAIFSKISTHFDGSYFIQNVREESEKSGGLAHLRQTLLS 270

Query: 61  EVLEERRDQLI-GNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIV 120
            +LE+    ++  NI   F  KR+  +KVLIV DDV+  EQ+E L G + W  PGSR+++
Sbjct: 271 AILEDGNVSIVCRNIGLNFRSKRLSRKKVLIVFDDVSTSEQMEFLIGDKGWLMPGSRLVI 330

Query: 121 TTRHRQVVN--GADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAG 180
           T R +QV+   G D +YE + L  ++A+ LFS +AF   G +H    ++ELS K + YA 
Sbjct: 331 TARDKQVLRNCGVDTIYEVKELFDDDALMLFSRYAF---GKNHPNADYMELSNKIIKYAK 390

Query: 181 GIPLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVAC 240
           G+PLA+KV G  LCG+    WES + K++R+P+  +Q VLKVS++GLD  E+ IFLD+AC
Sbjct: 391 GVPLAIKVFGRFLCGRRIQDWESAMNKMKRIPHVDIQRVLKVSYDGLDDEEQNIFLDIAC 450

Query: 241 FFKGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQED 300
           FFKG+         D CGF  E+G+SVL+DKCL+ I+ N++  MHDL++EMG+EIVRQE 
Sbjct: 451 FFKGEDKDCVIRFLDGCGFSSEIGISVLVDKCLMIISNNTIM-MHDLLQEMGREIVRQES 510

Query: 301 MEEPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNF 360
           +++PGKRSR               GT +I+GI LD+S+++++ L   +F  M KLRF   
Sbjct: 511 IKDPGKRSRLWHHEDIYNVLTKNMGTESIEGISLDMSEVEDIHLNARSFTNMHKLRFFKL 570

Query: 361 FSKNLGESTKTRIRLPHGLTMLPDS-LMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIK 420
           +S + GE+    +   H    L  + L YL W G P K   S   P+ L  L + HS IK
Sbjct: 571 YSSHYGEN----VNKVHNFRGLESTELRYLQWHGCPLKSLSSKIHPENLVSLEMPHSSIK 630

Query: 421 QLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLK 480
           QL KG +   NLKH+NLS SE+L+++PD+S A  LE +NF GC  L   H S+  L+KL 
Sbjct: 631 QLWKGVQRLVNLKHLNLSHSEHLTKIPDLSLATNLESLNFQGCTSLLETHSSIQYLNKLI 690

Query: 481 TLNLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLP 540
            LNL  CR L +L +S  L+SL++L LSGCS+L +FP +S  +E L   SL GTAI  LP
Sbjct: 691 VLNLGHCRSLTSLSTSTHLESLKKLILSGCSNLMSFPELSCNIEEL---SLDGTAIQELP 750

Query: 541 SSIENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTV 600
           SSIE    L+ L+L NC  L  LP  IC            C NLQ LP  +GNLE+L  +
Sbjct: 751 SSIERLSSLILLNLGNCSRLEGLPSKIC------------CSNLQRLPNELGNLEALKEL 810

Query: 601 YVDETSIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLP 660
             +  +I++VPSSIVCLKN+  LSF   +G E                      MGL LP
Sbjct: 811 KAEGIAIREVPSSIVCLKNLGRLSFESFKGHE---------------------QMGLLLP 870

Query: 661 PLGGFKQLSSLSLSNCNLVEAPSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLC 720
              G   L+ L L++C++ E P  L  L  L  + L +NNFE +P+SI  L  L  L + 
Sbjct: 871 ISFGLTSLTYLRLTDCSITELPECLGQLSSLRILFLDKNNFERIPESIICLSHLYWLRIS 915

Query: 721 YCKKLVSLPKLPSSLSVLLADECTSLESI 729
           YC++L SLP+LP  LS + A  C+SLE++
Sbjct: 931 YCERLKSLPELPCDLSDIEAHCCSSLEAL 915

BLAST of MC04g2000 vs. ExPASy TrEMBL
Match: V4VPL7 (NB-ARC domain-containing protein OS=Citrus clementina OX=85681 GN=CICLE_v10018644mg PE=4 SV=1)

HSP 1 Score: 573 bits (1478), Expect = 1.67e-188
Identity = 327/756 (43.25%), Postives = 461/756 (60.98%), Query Frame = 0

Query: 1   SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHG-LSHLQEKLLC 60
           S+DV  LGIWG+GGIGKTTLA A+F+RI +QF+G  FL+NVRE   K G LS L++KL  
Sbjct: 69  SKDVYTLGIWGIGGIGKTTLAGAIFNRISNQFEGSYFLQNVREESEKTGGLSQLRQKLFS 128

Query: 61  EVLEERRDQLIG--NISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRII 120
            +LE+    ++G  N+   F  KR   +K++IV DDV   EQ+  L G  DWF  GSRII
Sbjct: 129 GLLEDE-SPIVGVPNVGLNFRGKRFSRKKIIIVFDDVTCSEQITFLIGSLDWFASGSRII 188

Query: 121 VTTRHRQVVNGA--DEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYA 180
           +TTR +QV+     D +YE + L    A++LFS HAF      ++   + ELS + + +A
Sbjct: 189 ITTRDKQVLKNCRVDGIYEVEALLDYYALQLFSRHAFGQN--QNADPSYKELSDRIIKFA 248

Query: 181 GGIPLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVA 240
            G+PLALKVLG  L G+    WES   KL+++P+  +Q VLK S++GLD  E+ IFLD+A
Sbjct: 249 QGVPLALKVLGCFLFGRKMEDWESAANKLKKVPHLDIQKVLKASYDGLDDEEQNIFLDIA 308

Query: 241 CFFKGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQE 300
           CFFKG+   +     DA GF  E+G+SVL+DK L+ I +N +  MHDL++ MG+EIVRQE
Sbjct: 309 CFFKGEDKDLVVEFLDASGFSAEIGISVLVDKSLIIILKNKI-TMHDLLQRMGREIVRQE 368

Query: 301 DMEEPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLN 360
            +++PGKRSR               GT  I+GI LD+SK+K++ L    F +M KLRFL 
Sbjct: 369 SIKDPGKRSRLWNHEDIYHVLTRNKGTETIEGISLDMSKVKDINLNPQTFIKMHKLRFLK 428

Query: 361 FFSKNLGESTKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIK 420
           F++   GE  K ++    GL  +   L Y  W+GYP K  PS    + L  L + HS ++
Sbjct: 429 FYNSVDGEH-KNKVHHFQGLDYVFSELKYFHWNGYPLKAMPSYIHQENLIALEMPHSSVE 488

Query: 421 QLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLK 480
           +L  G +   NLK+++LS S+ L+E+PD+S A  +E +N  GC  L  IHPS+  L+KL 
Sbjct: 489 KLWGGAQQLLNLKYMDLSHSKQLTEIPDLSLASNIEKLNLDGCSSLLEIHPSIKYLNKLA 548

Query: 481 TLNLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLP 540
            L+LR C+ +++LP+SI L+SL++L LSGCS+L  FP I+  +E L+   L GTAI  LP
Sbjct: 549 ILSLRHCKCIKSLPTSIHLESLKQLFLSGCSNLNTFPEIACTIEELF---LDGTAIEELP 608

Query: 541 SSIENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTV 600
            SIE    L+ L+L NC  L  L  S C LK L  L LS C  ++ LP+  GNLE+L  +
Sbjct: 609 LSIECLSRLITLNLENCSRLECLSSSFCKLKSLQHLNLSGCTKVERLPDEFGNLEALREM 668

Query: 601 YVDETSIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLP 660
             + +SI++VPSSIV L N++ LSF G +                     S  +MGL+LP
Sbjct: 669 KAERSSIREVPSSIVQLNNLYRLSFEGYQ---------------------SKSHMGLRLP 728

Query: 661 PLGGFKQLSSLSLSNCNLVEAPSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLC 720
            + G + L++L LS+C + E P+ L  L  L  + L+ NNFE +P SI  L  L  L L 
Sbjct: 729 TMSGLRILTNLDLSDCGITELPNSLGQLSSLYILFLNRNNFERIPTSIIHLTNLLWLDLT 788

Query: 721 YCKKLVSLPKLPSSLSVLLADECTSLESIMSFEFFY 736
           YC++L SLP+LP ++S + A+ CTSL+ +  F   +
Sbjct: 789 YCERLQSLPELPCNISYMDANCCTSLKELSGFSILF 795

BLAST of MC04g2000 vs. ExPASy TrEMBL
Match: A0A5N5HVJ6 (TMV resistance protein N-like OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_009198 PE=4 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 3.89e-188
Identity = 344/751 (45.81%), Postives = 468/751 (62.32%), Query Frame = 0

Query: 3   DVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCEVL 62
           DV I+GI GMGGIGKTT+AR L+DRI  QFDG  FL NVRE   K GL  LQ +++ E+L
Sbjct: 210 DVRIIGICGMGGIGKTTIARVLYDRIWGQFDGSSFLSNVREVSEKRGLVSLQRQMISEIL 269

Query: 63  EERRDQLIGNISRG--FLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTT 122
            E     + ++ +G   ++ R+R R+VL++LDDV+  EQLE LAG  DWFG GSRII+TT
Sbjct: 270 METNVN-VWDVFKGSSVIRHRLRHRRVLLILDDVDQSEQLEKLAGKHDWFGLGSRIIITT 329

Query: 123 R--HRQVVNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGI 182
           R  H  + +G D++Y+A+ L+  E++ LFS  AF     DH  + +L+LS   +NYA G+
Sbjct: 330 RDEHLLLRHGVDQIYKAKELNQNESLELFSWKAFKK---DHPEKEYLDLSYLVLNYANGL 389

Query: 183 PLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFF 242
           PLAL+VLGS L  ++   W++ L KL+ +PN  V ++L++SFEGL+  E+ +FLD+ACFF
Sbjct: 390 PLALEVLGSFLFRRNVSEWKTALDKLKEVPNITVFDILRISFEGLEEMERNMFLDIACFF 449

Query: 243 KGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDME 302
           K  +      + D+ GF  ++G+ VL DK L+S++ N ++ MHDL++EMG EIVRQE   
Sbjct: 450 KWKNKARVTKILDSFGFHSDIGIRVLSDKSLISVSYNMLW-MHDLLQEMGWEIVRQESRS 509

Query: 303 EPGKRSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFS 362
           EPGKRSR               GT  ++G++L     +EV    +AF++M KLR L   +
Sbjct: 510 EPGKRSRLWLFEDVYDVLVNNKGTEVVEGLVLTSRADEEVHTSTEAFSKMNKLRMLKLGN 569

Query: 363 KNLGESTKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLS 422
            +L E           LT L + L  L W GYP +  PSNF P+KLF L L +S I++L 
Sbjct: 570 VHLSEE----------LTYLSNELRVLKWHGYPSRSLPSNFRPEKLFELSLCNSRIEELW 629

Query: 423 KG-QKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTL 482
           K  +K    LK I LS S+ L + PD SE P LE +   GC  L  IHPS+  L +L  L
Sbjct: 630 KVIKKPLEKLKIIKLSYSQSLIKTPDFSEVPNLERLILEGCTSLSDIHPSITGLKRLVLL 689

Query: 483 NLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSS 542
           NL+DC+ LR+LPSSI LKSL+   LSGC+ L+ FP I   MEHL+ LSL GTAI  LPS+
Sbjct: 690 NLKDCKSLRSLPSSIELKSLRIFVLSGCTKLKKFPKIVGNMEHLFKLSLDGTAIRELPST 749

Query: 543 IENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYV 602
           I++  GLV L + +CK LV+LP SIC L+ L  L LS C  L+ LP+N+G+LESL  + +
Sbjct: 750 IDHLPGLVYLSMRDCKGLVNLPSSICALESLKVLALSGCSKLEKLPDNLGHLESLEDLDI 809

Query: 603 DETSIKQVPSSIVCLKNIFELSFNGCRG---TETLSSLGFLSWFLPRSRSSSSCNMGLQL 662
             T+I++ P S+  LKN+  LSF GC G   T  +SSL   S  + R R  S   MG  +
Sbjct: 810 GGTAIREAPPSVELLKNLKLLSFRGCNGLPSTPWISSLW--STLVMRRRQDS---MGFLV 869

Query: 663 PPLGGFKQLSSLSLSNCNLVEA--PSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTL 722
           P L     L+ L LS+CNL E   PS L  L  L  ++L+ NNF+SLP SI  L +L  L
Sbjct: 870 PCLSSLHSLTELDLSDCNLSEGMLPSDLGCLSSLVKLNLNRNNFDSLPASISQLTKLTEL 929

Query: 723 MLCYCKKLVSLPKLPSSLSVLLADECTSLES 728
            L  CK+L  LP +PSS+  L+A +C SLE+
Sbjct: 930 NLNGCKRLQLLPDIPSSVRELMAQDCLSLET 940

BLAST of MC04g2000 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 497.3 bits (1279), Expect = 2.2e-140
Identity = 314/844 (37.20%), Postives = 465/844 (55.09%), Query Frame = 0

Query: 2    RDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCEV 61
            +DV +LGIWGMGG+GKTT+A+ L++++  QF   CF++NV+E   ++G+  LQ + LC +
Sbjct: 204  KDVRMLGIWGMGGVGKTTIAKYLYNQLSGQFQVHCFMENVKEVCNRYGVRRLQVEFLCRM 263

Query: 62   LEERRDQLIGNIS-RGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTT 121
             +ER  +   ++S    +K+R R + V IVLDDV+  EQL +L     WFGPGSRIIVTT
Sbjct: 264  FQERDKEAWSSVSCCNIIKERFRHKMVFIVLDDVDRSEQLNELVKETGWFGPGSRIIVTT 323

Query: 122  RHRQVV--NGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGI 181
            R R ++  +G + VY+ + L  +EA++LF ++AF  E I   P GF ELS +AVNYA G+
Sbjct: 324  RDRHLLLSHGINLVYKVKCLPKKEALQLFCNYAFREEII--LPHGFEELSVQAVNYASGL 383

Query: 182  PLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFF 241
            PLAL+VLGS L  + +  WES LA+L+  P+  +  VL+VS++GLD  EK IFL ++CF+
Sbjct: 384  PLALRVLGSFLYRRSQIEWESTLARLKTYPHSDIMEVLRVSYDGLDEQEKAIFLYISCFY 443

Query: 242  KGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDME 301
                V   + + D CG+  E+G+++L +K L+ +  N   ++HDL+E+MG+E+VRQ+ + 
Sbjct: 444  NMKQVDYVRKLLDLCGYAAEIGITILTEKSLI-VESNGCVKIHDLLEQMGRELVRQQAVN 503

Query: 302  EPGKR---------------SRGTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFS 361
             P +R               + GT  ++GI L+LS+I EV   + AF  ++ L+ LNF+ 
Sbjct: 504  NPAQRLLLWDPEDICHLLSENSGTQLVEGISLNLSEISEVFASDRAFEGLSNLKLLNFY- 563

Query: 362  KNLGESTKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLS 421
             +L    +TR+ LP+GL+ LP  L YL WDGYP K  PS F P+ L  L + +S +++L 
Sbjct: 564  -DLSFDGETRVHLPNGLSYLPRKLRYLRWDGYPLKTMPSRFFPEFLVELCMSNSNLEKLW 623

Query: 422  KGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLN 481
             G +   NLK ++LS  +YL EVPD+S+A  LE++N + C  L  + PS+  L  L    
Sbjct: 624  DGIQPLRNLKKMDLSRCKYLVEVPDLSKATNLEELNLSYCQSLVEVTPSIKNLKGLSCFY 683

Query: 482  LRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSI 541
            L +C +L+++P  I LKSL+ + +SGCSSL++FP IS     L+   LS T I  LPSSI
Sbjct: 684  LTNCIQLKDIPIGIILKSLETVGMSGCSSLKHFPEISWNTRRLY---LSSTKIEELPSSI 743

Query: 542  ENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYV- 601
                 LV LD+++C+ L +LP  + +L  L SL L  C  L++LP+ + NL SL T+ V 
Sbjct: 744  SRLSCLVKLDMSDCQRLRTLPSYLGHLVSLKSLNLDGCRRLENLPDTLQNLTSLETLEVS 803

Query: 602  --------------------DETSIKQVPS------------------------SIVCLK 661
                                 ETSI+++P+                        SI  L+
Sbjct: 804  GCLNVNEFPRVSTSIEVLRISETSIEEIPARICNLSQLRSLDISENKRLASLPVSISELR 863

Query: 662  NIFELSFNGCRGTETL-----SSLGFLSWF---------LPRSRSSSSCNMGLQ------ 721
            ++ +L  +GC   E+       ++  L WF         LP +  +      LQ      
Sbjct: 864  SLEKLKLSGCSVLESFPLEICQTMSCLRWFDLDRTSIKELPENIGNLVALEVLQASRTVI 923

Query: 722  --------------------------------LPPLGGFKQLSSLSLSNCNLVEAPSGLH 730
                                             PPL  F  L +LSLSN N+ E P+ + 
Sbjct: 924  RRAPWSIARLTRLQVLAIGNSFFTPEGLLHSLCPPLSRFDDLRALSLSNMNMTEIPNSIG 983

BLAST of MC04g2000 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 447.2 bits (1149), Expect = 2.6e-125
Identity = 290/759 (38.21%), Postives = 419/759 (55.20%), Query Frame = 0

Query: 4   VCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCEVLE 63
           V I+GI GM GIGKTTLA  L+ R++ QFDG CFL N+REN  + GL  L +KL   VL 
Sbjct: 209 VHIIGIVGMVGIGKTTLADCLYGRMRGQFDGSCFLTNIRENSGRSGLESLLQKLFSTVLN 268

Query: 64  ERRDQL--IGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTTR 123
           +R  ++   GN    F ++R++ +++LIVLDDVND +Q+  L G   W+  GSRII+TTR
Sbjct: 269 DRDLEIGAPGNAHERF-ERRLKSKRLLIVLDDVNDEKQIRYLMGHCKWYQGGSRIIITTR 328

Query: 124 HRQVVNG-ADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPL 183
             +++       Y    L+  EA++LFS +AF+        EG   L+   ++YA G PL
Sbjct: 329 DSKLIETIKGRKYVLPKLNDREALKLFSLNAFSNSFPLKEFEG---LTNMVLDYAKGHPL 388

Query: 184 ALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKG 243
           ALKVLGS LC +D   WE+KL +L+   +  +  VL+ S+E L   +K +FLD+ACFF+ 
Sbjct: 389 ALKVLGSDLCERDDLYWEAKLDRLKSRSHGDIYEVLETSYEELTTEQKNVFLDIACFFRS 448

Query: 244 DHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEI--------- 303
           ++V    ++ ++ G      +  L+DKCL++++ N + +MHD+++ M KEI         
Sbjct: 449 ENVDYVTSLLNSHGVDVSGVVKDLVDKCLITLSDNRI-EMHDMLQTMAKEISLKVETIGI 508

Query: 304 ----------------VR---QEDMEEPGKRSRGTNAIKGILLDLSKIKEVELGEDAFAE 363
                           +R    ED+ +      GT+ I+GI LD SK++ + L   AF  
Sbjct: 509 RDCRWLSRHGNQCQWHIRLWDSEDICDLLTEGLGTDKIRGIFLDTSKLRAMRLSAKAFQG 568

Query: 364 MTKLRFLNFFSKNL--GESTKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLF 423
           M  L++L  +  +   G   + ++ L  GL+ LP+ L YL W GYP +  P +FDP  L 
Sbjct: 569 MYNLKYLKIYDSHCSRGCEAEFKLHLRRGLSFLPNELTYLHWHGYPLQSIPLDFDPKNLV 628

Query: 424 WLRLRHSCIKQLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIH 483
            L+L HS ++++   +K    LK ++LS S  L +   ++ A  LE +N  GC  L  + 
Sbjct: 629 DLKLPHSQLEEIWDDEKDVGMLKWVDLSHSINLRQCLGLANAHNLERLNLEGCTSLKKLP 688

Query: 484 PSMATLSKLKTLNLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLS 543
            ++  L KL  LNLRDC  LR+LP  I  +SLQ L LSGCSSL+ FP IS+ +E    L 
Sbjct: 689 STINCLEKLIYLNLRDCTSLRSLPKGIKTQSLQTLILSGCSSLKKFPLISENVE---VLL 748

Query: 544 LSGTAISNLPSSIENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPEN 603
           L GT I +LP SI+ F  L  L+L NCK L  L   +  LKCL  LILS C  L+  PE 
Sbjct: 749 LDGTVIKSLPESIQTFRRLALLNLKNCKKLKHLSSDLYKLKCLQELILSGCSQLEVFPEI 808

Query: 604 IGNLESLSTVYVDETSIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSS 663
             ++ESL  + +D+TSI ++P  ++ L NI   S  G     T S +    +F       
Sbjct: 809 KEDMESLEILLMDDTSITEMP-KMMHLSNIKTFSLCG-----TSSHVSVSMFF------- 868

Query: 664 SSCNMGLQLPPLGGFKQLSSLSLSNCNLVEAPSGLHNLPCLSSIDLSENNFESLPQSIYD 723
                   +PP  G  +L+ L LS C+L + P  +  L  L S+ LS NN E+LP+S   
Sbjct: 869 --------MPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQSLCLSGNNIENLPESFNQ 928

Query: 724 LPELGTLMLCYCKKLVSLPKLPSSLSVLLADECTSLESI 730
           L  L    L +CK L SLP LP +L  L A EC SLE++
Sbjct: 929 LNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETL 938

BLAST of MC04g2000 vs. TAIR 10
Match: AT4G19510.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 393.7 bits (1010), Expect = 3.4e-109
Identity = 262/744 (35.22%), Postives = 399/744 (53.63%), Query Frame = 0

Query: 4   VCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCEVLE 63
           V ++G+ GM GIGKTT+A  ++ +   +FDG  FL+++ +N  ++GL +L +KLL ++L+
Sbjct: 254 VRVVGVLGMTGIGKTTVADIVYKQNFQRFDGYEFLEDIEDNSKRYGLPYLYQKLLHKLLD 313

Query: 64  ERRDQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTTRHR 123
               + +   ++G  +  +R +K+ IVLD+V + +Q+E L G ++ +  GSRI++ TR +
Sbjct: 314 ---GENVDVRAQGRPENFLRNKKLFIVLDNVTEEKQIEYLIGKKNVYRQGSRIVIITRDK 373

Query: 124 QVV-NGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPLAL 183
           +++   AD  Y    L+  EAM LF    F   G  +  E F++LS   V YA G+PLAL
Sbjct: 374 KLLQKNADATYVVPRLNDREAMELFCLQVF---GNHYPTEEFVDLSNDFVCYAKGLPLAL 433

Query: 184 KVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKGDH 243
           K+LG  L   D   W+ KL  L+  P++++Q  LK S++ LD  +K +FLD+ACFF+   
Sbjct: 434 KLLGKGLLTHDINYWKKKLEFLQVNPDKELQKELKSSYKALDDDQKSVFLDIACFFR--- 493

Query: 244 VGIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEPGK 303
                                               +MHDL+  MGKEI +++ + + G+
Sbjct: 494 -----------------------------------IEMHDLLHAMGKEIGKEKSIRKAGE 553

Query: 304 RSR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLG 363
           R R               GT  ++GI L++S+++ ++L   AF  ++KL+FL F S +  
Sbjct: 554 RRRLWNHKDIRDILEHNTGTECVRGIFLNMSEVRRIKLFPAAFTMLSKLKFLKFHSSHCS 613

Query: 364 E--STKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKG 423
           +        +        PD L+YL W GYPY   PS+FDP +L  L LR+S IKQL + 
Sbjct: 614 QWCDNDHIFQCSKVPDHFPDELVYLHWQGYPYDCLPSDFDPKELVDLSLRYSHIKQLWED 673

Query: 424 QKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLR 483
           +K   +L+ ++L  S+ L  +  +S A  LE ++  GC  L  +  S+  +++L  LNLR
Sbjct: 674 EKNTESLRWVDLGQSKDLLNLSGLSRAKNLERLDLEGCTSL-DLLGSVKQMNELIYLNLR 733

Query: 484 DCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIEN 543
           DC  L +LP    +KSL+ L LSGC  L++F  IS+ +E   SL L GTAI  +   IE+
Sbjct: 734 DCTSLESLPKGFKIKSLKTLILSGCLKLKDFHIISESIE---SLHLEGTAIERVVEHIES 793

Query: 544 FYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDET 603
            + L+ L+L NC+ L  LP+ +  LK L  L+LS C  L+ LP     +E L  + +D T
Sbjct: 794 LHSLILLNLKNCEKLKYLPNDLYKLKSLQELVLSGCSALESLPPIKEKMECLEILLMDGT 853

Query: 604 SIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGF 663
           SIKQ P  + CL N+   SF  CR     S+                   GL + P  G 
Sbjct: 854 SIKQTP-EMSCLSNLKICSF--CRPVIDDST-------------------GLVVLPFSGN 913

Query: 664 KQLSSLSLSNCNLVEAPSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCYCKKL 723
             LS L L+NCN+ + P    +L  L  + LS NN E+LP+SI  L  L  L L +C +L
Sbjct: 914 SFLSDLYLTNCNIDKLPDKFSSLRSLRCLCLSRNNIETLPESIEKLYSLLLLDLKHCCRL 927

Query: 724 VSLPKLPSSLSVLLADECTSLESI 730
            SLP LPS+L  L A  C SLE++
Sbjct: 974 KSLPLLPSNLQYLDAHGCGSLENV 927

BLAST of MC04g2000 vs. TAIR 10
Match: AT5G18360.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 386.7 bits (992), Expect = 4.1e-107
Identity = 236/634 (37.22%), Postives = 368/634 (58.04%), Query Frame = 0

Query: 1   SRDVCILGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLS------HLQ 60
           S +V ++GIWG  GIGKTT+ARALF+R+   F    F++NV+ +     L        LQ
Sbjct: 204 STEVKMVGIWGPAGIGKTTIARALFNRLSENFQHTIFMENVKGSSRTSELDAYGFQLRLQ 263

Query: 61  EKLLCEVLEERRDQLIGNISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGS 120
           E+ L EV++ +  ++      G +K+R++  KVL+VLDDV+ +EQL+ L     WFG GS
Sbjct: 264 EQFLSEVIDHKHMKI---HDLGLVKERLQDLKVLVVLDDVDKLEQLDALVKQSQWFGSGS 323

Query: 121 RIIVTTRHRQVV--NGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAV 180
           RIIVTT ++Q++  +G   +YE    S  +++++F  +AF   G   +P+G +EL+T+  
Sbjct: 324 RIIVTTENKQLLRAHGITCIYELGFPSRSDSLQIFCQYAF---GESSAPDGCIELATEIT 383

Query: 181 NYAGGIPLALKVLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFL 240
             AG +PLALKVLGS L G  K   +S L +L    NE ++NVL+V ++G+   +K IFL
Sbjct: 384 KLAGYLPLALKVLGSSLRGMSKDEQKSALPRLRTSLNEDIRNVLRVGYDGIHDKDKVIFL 443

Query: 241 DVACFFKGDHVGIAKAMWDACGFFPEVGLSVLIDKCLLSITR-NSVFQMHDLIEEMGKEI 300
            +AC F G++V   K +  + G     GL VL  + L+ I+R N    MH+L+E++G+EI
Sbjct: 444 HIACLFNGENVDYVKQILASSGLDVTFGLQVLTSRSLIHISRCNRTITMHNLLEQLGREI 503

Query: 301 VRQEDMEEPGKR---------------SRGTNAIKGILLDLSKIKEVELGEDAFAEMTKL 360
           V ++ + EPGKR               + GT A+ GI LD+SKI E+ L E AF  M  L
Sbjct: 504 VCEQSIAEPGKRQFLMDASEIYDVLADNTGTGAVLGISLDISKINELFLNERAFGGMHNL 563

Query: 361 RFLNFFSKNLGESTKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRH 420
            FL F+ K+     +  + LP GL  LP  L  L WD +P    P +F P  L  + +R 
Sbjct: 564 LFLRFY-KSSSSKDQPELHLPRGLDYLPRKLRLLHWDAFPMTSMPLSFCPQFLVVINIRE 623

Query: 421 SCIKQLSKGQKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATL 480
           S +++L +G +   +LK ++LS SE L E+PD+S+A  +E++  + C  L  +  S+  L
Sbjct: 624 SQLEKLWEGTQPLRSLKQMDLSKSENLKEIPDLSKAVNIEELCLSYCGSLVMLPSSIKNL 683

Query: 481 SKLKTLNLRDCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAI 540
           +KL  L+++ C KL  +P ++ L+SL  L+L GCS L++FP IS     +  LSLS TAI
Sbjct: 684 NKLVVLDMKYCSKLEIIPCNMDLESLSILNLDGCSRLESFPEIS---SKIGFLSLSETAI 743

Query: 541 SNLPSSIENFYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLES 600
             +P+++ ++  L  LD++ CKNL + P   C  K ++ L LS  E ++ +P  I  L  
Sbjct: 744 EEIPTTVASWPCLAALDMSGCKNLKTFP---CLPKTIEWLDLSRTE-IEEVPLWIDKLSK 803

Query: 601 LSTVYVDE-TSIKQVPSSIVCLKNIFELSFNGCR 610
           L+ + ++    ++ + S I  L++I  L F GC+
Sbjct: 804 LNKLLMNSCMKLRSISSGISTLEHIKTLDFLGCK 823

BLAST of MC04g2000 vs. TAIR 10
Match: AT5G45200.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 377.5 bits (968), Expect = 2.5e-104
Identity = 249/744 (33.47%), Postives = 382/744 (51.34%), Query Frame = 0

Query: 7   LGIWGMGGIGKTTLARALFDRIKSQFDGCCFLKNVRENQIKHGLSHLQEKLLCEVLEERR 66
           +G+ GM GIGKTTLA+ LF      F    FL +V +         L   LL  + + + 
Sbjct: 240 IGVVGMPGIGKTTLAKRLFSECGKHFLHKMFLDDVSQKPEPFLDETLHTDLLLGLWKSKN 299

Query: 67  DQLIGN---ISRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTTRHR 126
           +   GN   +S  ++K +++G+KV +VLD+V D  Q++ + GG DW   GSRI++TT  +
Sbjct: 300 NGRDGNRAKLSIDYIKTQLQGKKVFVVLDNVGDKSQIDKILGGCDWIKAGSRIVITTSSK 359

Query: 127 QVVNGADEVYEAQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPLALK 186
            V+ G +  Y    LS  +A+  F++HAF+     + P  F +L+ + V+Y+ G P  LK
Sbjct: 360 SVIQGLNSTYLVPGLSSCDALNHFNYHAFSASDGFYQP-SFTDLAKQFVDYSMGHPSVLK 419

Query: 187 VLGSCLCGKDKPVWESKLAKLERLPNEKVQNVLKVSFEGLDRTEKGIFLDVACFFKGDHV 246
           +L   L  KD+  W+ KL+ L   P+  +Q+VL++ ++ L    K +FLD+A FF+ ++ 
Sbjct: 420 LLARELRSKDESYWKEKLSALANSPSNTIQDVLRIPYDELKEQHKIVFLDIAYFFRFENE 479

Query: 247 GIAKAMWDACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEPGKR 306
              + +  +        ++ L DK L+ I+ + V +M+DL+      +  Q   E     
Sbjct: 480 SYVRRLLGSSAHADASEITDLADKFLIDISGDRV-EMNDLLYTFAIGLNSQASSENTTSE 539

Query: 307 SR---------------GTNAIKGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLG- 366
            R                   ++G+ LD+ ++KE+ L  D F +M  LR+L F++ +   
Sbjct: 540 RRLSKHSEIVDVLMNKAEATKVRGVYLDMFEVKEMGLDSDTFNKMDDLRYLKFYNSHCHR 599

Query: 367 --ESTKTRIRLPHGLTMLPDSLMYLSWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKG 426
             E+  +++  P GL  LP  L YL+W  YP K  P NFDP  L  L+L +S I+Q+ + 
Sbjct: 600 ECEAEDSKLNFPEGLEFLPQELRYLNWLKYPEKNLPINFDPKNLIDLKLPYSQIEQIWEE 659

Query: 427 QKIFTNLKHINLSDSEYLSEVPDVSEAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLR 486
           +K  +NL+ ++L+ S  L  +  +S A  L+ IN  GC  L T+   +  +  L  LNLR
Sbjct: 660 EKDTSNLQWLDLNHSSKLHSLSGLSRAQKLQSINLEGCTGLKTLPQVLQNMESLMFLNLR 719

Query: 487 DCRKLRNLPSSICLKSLQELDLSGCSSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIEN 546
            C  L +LP  I L  L+ L LS CS  + F  I+  +E L+   L GTAI  LPS+I +
Sbjct: 720 GCTSLESLP-DITLVGLRTLILSNCSRFKEFKLIAKNLEELY---LDGTAIKELPSTIGD 779

Query: 547 FYGLVGLDLTNCKNLVSLPDSICNLKCLDSLILSSCENLQHLPENIGNLESLSTVYVDET 606
              L+ L L +CKNL+SLPDSI NLK +  +ILS C +L+  PE   NL+ L T+ +D T
Sbjct: 780 LQKLISLKLKDCKNLLSLPDSIGNLKAIQEIILSGCSSLESFPEVNQNLKHLKTLLLDGT 839

Query: 607 SIKQVPSSIVCLKNIFELSFNGCRGTETLSSLGFLSWFLPRSRSSSSCNMGLQLPPLGGF 666
           +IK++P  +  L                                  S + GL        
Sbjct: 840 AIKKIPDILHHL----------------------------------SPDQGL-------- 899

Query: 667 KQLSSLSLSNCNLVEAPSGLHNLPCLSSIDLSENNFESLPQSIYDLPELGTLMLCYCKKL 726
               + S SNC+L E P G++ L  +  + LS N F  LP+SI  L  L  L L +CK L
Sbjct: 900 ----TSSQSNCHLCEWPRGIYGLSSVRRLSLSSNEFRILPRSIGYLYHLNWLDLKHCKNL 931

Query: 727 VSLPKLPSSLSVLLADECTSLESI 730
           VS+P LP +L  L A  C SLE+I
Sbjct: 960 VSVPMLPPNLQWLDAHGCISLETI 931