MC04g1963 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g1963 vs. ExPASy Swiss-Prot
Match: Q9LJB4 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase OS=Arabidopsis thaliana OX=3702 GN=5MAT PE=1 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.5e-75
Identity = 174/458 (37.99%), Postives = 257/458 (56.11%), Query Frame = 0

Query: 6   LEILEVCKISPSSPAADFSLPLTFFDLFFLASSPTERILYYH-HHFDSSSVLPKLKRSLS 65
           + ILEV ++SP S +   +LPLT+FDL +L   P +R+L+YH      SS++ KLK SLS
Sbjct: 8   VNILEVVQVSPPS-SNSLTLPLTYFDLGWLKLHPVDRVLFYHVPELTRSSLISKLKSSLS 67

Query: 66  LALSHFLPLAGNLVWPDQSPEPIILYKPG--DAVSLTIAQSDADFSRLSADHVRKATDSH 125
             L H+LPLAG LVW     +P I+Y P   DAV LT+A+S+ D S LS D  R AT+ H
Sbjct: 68  ATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEPRPATEFH 127

Query: 126 FLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKSWASICKL 185
            LVP+L  SD+S    + +Q+T FP    GFS+G+  +H V+DGKT+ +FLK+WA  CK 
Sbjct: 128 SLVPELPVSDESA-RVLAVQVTFFPNQ--GFSLGVTAHHAVLDGKTTAMFLKAWAHNCKQ 187

Query: 186 CLSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSRKLRLTPKVAISDDL 245
              +LP +L P  DR  ++DP G        K L  ++S  S     L+L P   I  D+
Sbjct: 188 EQEALPHDLVPSLDRIIVQDPTG-----LETKLLNRWIS-ASNNKPSLKLFPSKIIGSDI 247

Query: 246 VTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRIKAERLEPKSKVGLFF 305
           +  T+ +TR  ++ LR +    + + +  L+TFV+  A+V  C +K    +P   V + F
Sbjct: 248 LRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTRFVCVGF 307

Query: 306 PVDWRLRAGAPVGPNYFGNGVCGYG-FLTEAREFEEE-------NAMEIIQSKISDAIAE 365
             D+R R   P+ P +FGN + G G F  +A    EE        A+E +   ++    E
Sbjct: 308 ASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFITAVETLTGWVNGLCPE 367

Query: 366 VTEEKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKRVEMSSITPD 425
             E+      M++   A    E P  + I  AGS R G+Y +DFGWG+  +VE+ +I  D
Sbjct: 368 NIEK-----NMLLPFEAFKRME-PGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTIDKD 427

Query: 426 GTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 453
            ++S+ ES +G+GG+E+G+ L +  +  F SLF  GLE
Sbjct: 428 ASVSLSESGDGSGGVEVGVCLKKDDVERFGSLFSIGLE 449

BLAST of MC04g1963 vs. ExPASy Swiss-Prot
Match: Q940Z5 (Phenolic glucoside malonyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PMAT1 PE=1 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 3.6e-74
Identity = 175/468 (37.39%), Postives = 254/468 (54.27%), Query Frame = 0

Query: 6   LEILEVCKISPSS--PAADFSLPLTFFDLFFLASSPTERILYY-----HHHFDSSSVLPK 65
           L++++V +++PS+   +   +LPLTFFDL +      ER+++Y        F  S ++P 
Sbjct: 10  LKVIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIVPN 69

Query: 66  LKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADHVRKA 125
           LK SLS +LSH+LPLAG LVW    P+P I+Y P DAVS T+A+S+ADFSRL+       
Sbjct: 70  LKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKEPFPT 129

Query: 126 TDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKSWAS 185
           T+ + LVP+L+ SDDS   A+  Q+TLFP    GF I +  +H V+DGKT+T FLKSWA 
Sbjct: 130 TELYPLVPELHVSDDSA-SAVSFQVTLFPNQ--GFCISVNAHHAVLDGKTTTNFLKSWAR 189

Query: 186 ICKLCLSSLPPELTPDFDRTAIRDPVG------SGWQTAYEKFLGIFLSPTSTGSRKLRL 245
            CK   S LP +L P +DRT I+DP+       + W    + F G    P +  S KL  
Sbjct: 190 TCKNQDSFLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTG-GKEPENPKSLKLLW 249

Query: 246 TPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSST-------RRHLTTFVLALAHVSIC 305
           +P+  I  D+   T ++TR  ++ LR +    +SS+          L+TFV+  ++   C
Sbjct: 250 SPE--IGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYALTC 309

Query: 306 RIKAERLEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCG-YGFLTEAREFEEENAMEII 365
            IKA   +P   VG  F VD R     PV  +YFGN V   +     A  F  E      
Sbjct: 310 LIKARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFLAA 369

Query: 366 QSKISDAIAEVTEEKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCK 425
              +SD++  + E        + E+        P  + +  AGS RFGVY  DFGWGR +
Sbjct: 370 ARMVSDSVEALDE---NVALKIPEILEGFTTLSPGTQVLSVAGSTRFGVYGLDFGWGRPE 429

Query: 426 RVEMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 453
           +V + SI     +S  ESR+G+GG+E+G  L +H ++    L ++GLE
Sbjct: 430 KVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLE 468

BLAST of MC04g1963 vs. ExPASy Swiss-Prot
Match: Q9LRQ8 (Phenolic glucoside malonyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PMAT2 PE=1 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 7.0e-70
Identity = 174/464 (37.50%), Postives = 247/464 (53.23%), Query Frame = 0

Query: 6   LEILEVCKISPS-----SPAADFSLPLTFFDLFFLASSPTERILYY------HHHFDSSS 65
           L ++E  +++P+     + A    LPLTFFDL +L   P +R+ +Y        HF  S 
Sbjct: 3   LHVIETARVTPTDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDHF-HSI 62

Query: 66  VLPKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADH 125
           +LPKLK SLSL L ++LPL G++ W    P+P I+      V +TIA+SDADFS LS   
Sbjct: 63  ILPKLKDSLSLILRNYLPLTGHITWEPNEPKPSIIVSENGVVLVTIAESDADFSHLSGYG 122

Query: 126 VRKATDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLK 185
            R  ++ H LVP+L  SDDS   A  +QITLFP    GFSIG+  +H V+DGKTS+ F+K
Sbjct: 123 QRPLSELHALVPKLPVSDDSA-TAFSIQITLFPNQ--GFSIGVAAHHAVLDGKTSSTFIK 182

Query: 186 SWASICKLCLSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIF--LSPTSTGSRKLRL 245
           +WA ICK  L S+P  LTP +DR+ I+ P         EK + +   L    T  R L  
Sbjct: 183 AWAQICKQELQSMPENLTPSYDRSLIKYPT-----YLDEKMIELVRSLKEDQTNIRSLTS 242

Query: 246 TPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRIKAERL 305
            P   + DD+V  T  ++R+ +E LR +  N + S   HL+TFV+A A+   C +KA   
Sbjct: 243 LPSSKLGDDVVLATLVLSRADIERLREQVKNVSPSL--HLSTFVIAYAYAWTCFVKARGG 302

Query: 306 EPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYG-FLTEAREFEEENAMEIIQSKISDA 365
                V L F  D+R R    +   YFGN +   G +  +A EF EE         ISD 
Sbjct: 303 NKDRSVSLLFVGDFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAAEIISDL 362

Query: 366 IAEVTEEKNRADEMVVELAARLMKEMPF----CRTIMAAGSPRFGVYETDFGWGRCKRVE 425
           +  ++  K      +  +A   ++   F     +    AGS R GVYE DFGWGR  +V+
Sbjct: 363 VKGLSSRK------IETIADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVKVD 422

Query: 426 MSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGL 452
           + SI     ++M E R+ +GG+EIG+ L +  ++   S F  GL
Sbjct: 423 IVSIDQGEAIAMAERRDESGGVEIGMCLKKTEMDSVVSFFNNGL 449

BLAST of MC04g1963 vs. ExPASy Swiss-Prot
Match: Q9LRQ7 (BAHD acyltransferase At3g29680 OS=Arabidopsis thaliana OX=3702 GN=At3g29680 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.5e-67
Identity = 171/453 (37.75%), Postives = 249/453 (54.97%), Query Frame = 0

Query: 6   LEILEVCKIS-PSSPAADFSLPLTFFDLFFLASSPTERILYYHHHFDS-----SSVLPKL 65
           L ++++ ++S  ++      LPLTFFDL +L  +P ER+ +Y     S     SS+LPKL
Sbjct: 3   LNVIKISRVSLVTNSVEPLVLPLTFFDLLWLKLNPIERVTFYKLTESSRDSFFSSILPKL 62

Query: 66  KRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADHVRKAT 125
           ++SLSL LSHFLPL+G+L W  Q P+P I+  P D VSLT+ +S+ADFS +S+  +R  T
Sbjct: 63  EQSLSLVLSHFLPLSGHLKWNPQDPKPHIVIFPKDTVSLTVVESEADFSYISSKELRLET 122

Query: 126 DSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKSWASI 185
           +   LVP+L  S DS    + LQITLFP    GFSIG   +HVV+DGKT++ F KSWA I
Sbjct: 123 ELRPLVPELQVSSDSA-SLLSLQITLFPNQ--GFSIGTTVHHVVMDGKTASKFHKSWAHI 182

Query: 186 CKLCLSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSRKLRLTPKVAIS 245
           CK   +    +L    DRT I  P G   Q  ++  L  ++S     +R L L P   I 
Sbjct: 183 CKHGTTPQDFDLPTVLDRTVINVPAGLE-QKIFQ--LSSYISEEKDYARTLTLPPAKEID 242

Query: 246 DDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRIKAERLEPKSKVG 305
           +D+V  T  +T   +E L+ +A N ++ +  HL+TFV++ A+V  C +K+   +    V 
Sbjct: 243 NDVVRVTLELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGDANRPVR 302

Query: 306 LFFPVDWRLRAGAPVGPNYFGNGVC-----GYGFLTEAREFEEENAMEIIQSKISDAIAE 365
             +  D+R R   PV   YFGN V      GY   T   +    N +EI    +SD++  
Sbjct: 303 FMYAADFRNRLDPPVPLTYFGNCVLPIDFNGYKATTFLGKDGYVNGVEI----LSDSVRG 362

Query: 366 VTEEKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKRVEMSSITPD 425
           +      +   V E   + MK     + +   GS +FG+Y +DFGWGR  + ++ S+  +
Sbjct: 363 LGSRNIESIWEVYEDGTKNMK--LDTQNVTVTGSNQFGIYGSDFGWGRPVKTDVMSLYKN 422

Query: 426 GTLSMVESRNGNGGLEIGILLPQHAINDFSSLF 448
              SM   R+  GGLEIGI L +  +N F SLF
Sbjct: 423 NEFSMSARRDEIGGLEIGISLKKCEMNVFLSLF 443

BLAST of MC04g1963 vs. ExPASy Swiss-Prot
Match: Q9FNP9 (Agmatine coumaroyltransferase OS=Arabidopsis thaliana OX=3702 GN=ACT PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.6e-66
Identity = 160/457 (35.01%), Postives = 249/457 (54.49%), Query Frame = 0

Query: 6   LEILEVCKISPSSPAAD-FSLPLTFFDLFFLASSPTERILYYHHHFDS-------SSVLP 65
           L+++++ ++SP++ + D   +PL+FFDL +L  +PTE++ +Y     S       SS+LP
Sbjct: 3   LKVIKISRVSPATASVDPLIVPLSFFDLQWLKLNPTEQVFFYKLTESSSSRDVFYSSILP 62

Query: 66  KLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADHVRK 125
           KL+RSLSL L+HF    G+L W  Q P+P ++   GD +SLT+A++DADFSR+S   +R 
Sbjct: 63  KLERSLSLILTHFRLFTGHLKWDSQDPKPHLVVLSGDTLSLTVAETDADFSRISGRGLRP 122

Query: 126 ATDSHFLVPQL--YSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKS 185
             +   L+P+L  YS   +V   + LQ+TLFPK   GF IG   +HVV+DGKT+  F K+
Sbjct: 123 ELELRPLIPELPIYSDSGAV---VSLQVTLFPKQ--GFCIGTTAHHVVLDGKTAEKFNKA 182

Query: 186 WASICKLCLSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSRKLRLTP- 245
           WA  CK    ++P  L    DR+ +  P  +G +    + L           R L+L P 
Sbjct: 183 WAHTCK--HGTIPKILPTVLDRSVVNVP--AGLEQKMLELLPYLTEDDKENGRTLKLPPV 242

Query: 246 -KVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRIKAERLE 305
            ++   D+++  T  ++   +E L+ +A   ++    HL+TFV+  AHV  C +KA   +
Sbjct: 243 KEINAKDNVLRITIEISPENIEKLKERAKKESTRAELHLSTFVVTFAHVWTCMVKARSGD 302

Query: 306 PKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYGFLT-EAREFEEENAMEIIQSKISDAI 365
           P   V   +  D+R R   PV   YFG  V    F   +A+EF  E+        +SD++
Sbjct: 303 PNRPVRFMYAADFRNRLEPPVPVTYFGTCVLAMDFYKYKAKEFMGEDGFVNTVEILSDSV 362

Query: 366 AEVTEEKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKRVEMSSIT 425
             +  +   +   V E   + MK     + ++  GS + G+YETDFGWGR    E  SI 
Sbjct: 363 KRLASQGVESTWKVYEEGTKTMKW--GTQLLVVNGSNQIGMYETDFGWGRPIHTETMSIY 422

Query: 426 PDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQ 450
            +   SM + R+G GG+EIGI L +  ++ F SLFY+
Sbjct: 423 KNDEFSMSKRRDGIGGVEIGISLKKLEMDTFLSLFYK 448

BLAST of MC04g1963 vs. NCBI nr
Match: XP_022986172.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like, partial [Cucurbita maxima])

HSP 1 Score: 495 bits (1275), Expect = 6.32e-170
Identity = 272/466 (58.37%), Postives = 335/466 (71.89%), Query Frame = 0

Query: 1   QAMAPLEILEVCKISPS-SPAADFSLPLTFFDLFFLASSPTERILYYHHHFDS----SSV 60
           Q  A +E+LEVC I+PS SP   FSLPLTF DL    S PTERIL+Y  H +      S+
Sbjct: 5   QPKAIMEVLEVCNITPSTSPPTHFSLPLTFSDLVQFLSPPTERILFYTLHANHLPRFDSI 64

Query: 61  LPKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADHV 120
           L  LK SLSL LSHFLPLAGNLVWP  SP+P ILY  GD+VSLTIA+++ADF  LS++HV
Sbjct: 65  LQTLKHSLSLTLSHFLPLAGNLVWPHHSPDPFILYNHGDSVSLTIAKTEADFHHLSSNHV 124

Query: 121 RKATDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKS 180
           RKAT+SHFLVPQL +SD ++ PAM LQITLFPKS  GFSIGI+TNHVVVDGKTST+FLKS
Sbjct: 125 RKATESHFLVPQLPTSD-TIAPAMSLQITLFPKS--GFSIGIITNHVVVDGKTSTMFLKS 184

Query: 181 WASICKLC-------LSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSR 240
           WASIC          L +LP ELTP FDRTA +DP  +G Q  Y K  G F+        
Sbjct: 185 WASICSTLNCTNNNNLPTLPSELTPFFDRTAAKDP--NGLQRLYVKHYGAFVRQPKI--- 244

Query: 241 KLRLTPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRIK 300
            L LTPK  ISDD+V  TF +TR  +E LRRK   +++STR HLTTFVL  A VS C ++
Sbjct: 245 -LGLTPKRVISDDVVYATFELTRIDIEKLRRKVATNSTSTR-HLTTFVLTYALVSTCIVQ 304

Query: 301 AERLEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYGFLTEAREFEEENAMEIIQSKI 360
           AER+EPKSK+ LFF VD R R GA  G NYFGNGV G G + EA+E EEEN M ++ +KI
Sbjct: 305 AERIEPKSKIALFFAVDMRPRMGALGGLNYFGNGVIGLGEILEAKELEEENGMAMVANKI 364

Query: 361 SDAIAEVTE--EKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKRV 420
           SD I E+ +  ++N+  EMV E++ R  +EMP  + I+A GSPRFGVYE DFGWG  K+V
Sbjct: 365 SDRIMEMEKSVKENKVVEMVEEISDRWGREMPVSKMIVAGGSPRFGVYEIDFGWGNSKKV 424

Query: 421 EMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 452
           EM+S+ P+G+ SM ESR+GNGG+E+G++LP+  +N FSSLFY+ ++
Sbjct: 425 EMASMDPNGSFSMTESRSGNGGVEVGVVLPKDVMNVFSSLFYEEVK 460

BLAST of MC04g1963 vs. NCBI nr
Match: KAG7010988.1 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 491 bits (1265), Expect = 1.52e-168
Identity = 270/463 (58.32%), Postives = 329/463 (71.06%), Query Frame = 0

Query: 6   LEILEVCKISPS-SPAADFSLPLTFFDLFFLASSPTERILYYH------HHFDSSSVLPK 65
           +E+LEVC I+PS SP   FSLPLTF DL    S PTERIL+Y         FDS  +L  
Sbjct: 1   MEVLEVCNITPSTSPPTHFSLPLTFSDLVQFMSPPTERILFYSLPANDLPRFDS--ILQT 60

Query: 66  LKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADHVRKA 125
           LK SLSL LSHFLPLAGNLVWP  SPEP I+Y PGD+VSLTIA+++ADF  L ++HVRKA
Sbjct: 61  LKHSLSLTLSHFLPLAGNLVWPQHSPEPFIVYNPGDSVSLTIAKTEADFDHLLSNHVRKA 120

Query: 126 TDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKSWAS 185
            +SHFLVPQL +SD ++ PAM LQITLFPKS  GFSIGI+T+HVVVDGKTST+FLKSWAS
Sbjct: 121 KESHFLVPQLPTSD-TIAPAMSLQITLFPKS--GFSIGIITSHVVVDGKTSTMFLKSWAS 180

Query: 186 ICKLC-------LSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSRKLR 245
           IC          L +LPPELTP FDRTA +DP  +G +  Y K  G F+         L 
Sbjct: 181 ICSTLNCTNNNNLPTLPPELTPFFDRTAAKDP--NGLERLYVKHYGAFVRQPKI----LV 240

Query: 246 LTPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRIKAER 305
           LTPK  ISDD+V  TF +TR  +E LRRK   +++STR HLTTFVL  A +S C +KAER
Sbjct: 241 LTPKRVISDDVVHATFELTRIDIEKLRRKVATNSTSTR-HLTTFVLTYALISTCIVKAER 300

Query: 306 LEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYGFLTEAREFEEENAMEIIQSKISDA 365
           +EPKSK+ LFF VD R R GA  G NYFGNGV G G   EA+E EEEN M ++ +KISD 
Sbjct: 301 IEPKSKIALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVANKISDR 360

Query: 366 IAEVTE--EKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKRVEMS 425
           I E+ +  ++N+  EMV E+  R  +EMP  + I+A GSPRFGVYE DFGWG  K+VEM 
Sbjct: 361 IMEMEKSVKENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSKKVEMV 420

Query: 426 SITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 452
           SI P+G  SM ESR+GNGG+E+G++LP+  +N FSSLFY+ ++
Sbjct: 421 SINPNGNFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVK 451

BLAST of MC04g1963 vs. NCBI nr
Match: XP_023522403.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 491 bits (1265), Expect = 1.81e-168
Identity = 271/468 (57.91%), Postives = 331/468 (70.73%), Query Frame = 0

Query: 1   QAMAPLEILEVCKISPS-SPAADFSLPLTFFDLFFLASSPTERILYYH------HHFDSS 60
           Q  A +E+LEVC I+PS SP   FSLPLTF DL    S PTERIL+Y         FDS 
Sbjct: 1   QPKAIMEVLEVCNITPSTSPLTHFSLPLTFSDLVQFISPPTERILFYSLPANDLPRFDS- 60

Query: 61  SVLPKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSAD 120
            +L  LK SLSL LSHFLPLAGNLVWP  SPEP I+Y PGD+VSLTIA+++ADF  L ++
Sbjct: 61  -ILQTLKHSLSLTLSHFLPLAGNLVWPQHSPEPFIVYNPGDSVSLTIAKTEADFDHLLSN 120

Query: 121 HVRKATDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFL 180
           HVRKA +SHFLVPQL +SD ++ P M LQITLFPKS  GFSIGI+T+HVVVDGKTST+FL
Sbjct: 121 HVRKAKESHFLVPQLPTSD-TIAPPMSLQITLFPKS--GFSIGIITSHVVVDGKTSTMFL 180

Query: 181 KSWASICKLC-------LSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTG 240
           KSWASIC          L +LPPELTP FDRTA +DP  +G +  Y K  G F+      
Sbjct: 181 KSWASICSTLNCTNNNNLPTLPPELTPFFDRTAAKDP--NGLERLYVKHYGAFVRQPKI- 240

Query: 241 SRKLRLTPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICR 300
              L LTPK  ISDD+V  TF +TR  +E LRRK   +++STR HLTTFVL  A +S C 
Sbjct: 241 ---LVLTPKRVISDDVVHATFELTRIDIEKLRRKVATNSTSTR-HLTTFVLTYALISTCI 300

Query: 301 IKAERLEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYGFLTEAREFEEENAMEIIQS 360
           +KAER+EPKSK+ LFF VD R R GA  G NYFGNGV G G   EA+E EEEN M ++ +
Sbjct: 301 VKAERIEPKSKIALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVAN 360

Query: 361 KISDAIAEVTE--EKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCK 420
           KISD I E+ +  ++N+  EMV E+  R  +EMP  + I+A GSPRFGVYE DFGWG  K
Sbjct: 361 KISDRIMEMEKSVKENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSK 420

Query: 421 RVEMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 452
           +VEM SI P+G+ SM ESR+GNGG+E+G++LP+  +N FSSLFY+ ++
Sbjct: 421 KVEMVSINPNGSFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVK 456

BLAST of MC04g1963 vs. NCBI nr
Match: XP_023512233.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 491 bits (1265), Expect = 8.15e-168
Identity = 271/468 (57.91%), Postives = 331/468 (70.73%), Query Frame = 0

Query: 1   QAMAPLEILEVCKISPS-SPAADFSLPLTFFDLFFLASSPTERILYYH------HHFDSS 60
           Q  A +E+LEVC I+PS SP   FSLPLTF DL    S PTERIL+Y         FDS 
Sbjct: 45  QPKAIMEVLEVCNITPSTSPLTHFSLPLTFSDLVQFISPPTERILFYSLPANDLPRFDS- 104

Query: 61  SVLPKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSAD 120
            +L  LK SLSL LSHFLPLAGNLVWP  SPEP I+Y PGD+VSLTIA+++ADF  L ++
Sbjct: 105 -ILQTLKHSLSLTLSHFLPLAGNLVWPQHSPEPFIVYNPGDSVSLTIAKTEADFDHLLSN 164

Query: 121 HVRKATDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFL 180
           HVRKA +SHFLVPQL +SD ++ P M LQITLFPKS  GFSIGI+T+HVVVDGKTST+FL
Sbjct: 165 HVRKAKESHFLVPQLPTSD-TIAPPMSLQITLFPKS--GFSIGIITSHVVVDGKTSTMFL 224

Query: 181 KSWASICKLC-------LSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTG 240
           KSWASIC          L +LPPELTP FDRTA +DP  +G +  Y K  G F+      
Sbjct: 225 KSWASICSTLNCTNNNNLPTLPPELTPFFDRTAAKDP--NGLERLYVKHYGAFVRQPKI- 284

Query: 241 SRKLRLTPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICR 300
              L LTPK  ISDD+V  TF +TR  +E LRRK   +++STR HLTTFVL  A +S C 
Sbjct: 285 ---LVLTPKRVISDDVVHATFELTRIDIEKLRRKVATNSTSTR-HLTTFVLTYALISTCI 344

Query: 301 IKAERLEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYGFLTEAREFEEENAMEIIQS 360
           +KAER+EPKSK+ LFF VD R R GA  G NYFGNGV G G   EA+E EEEN M ++ +
Sbjct: 345 VKAERIEPKSKIALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVAN 404

Query: 361 KISDAIAEVTE--EKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCK 420
           KISD I E+ +  ++N+  EMV E+  R  +EMP  + I+A GSPRFGVYE DFGWG  K
Sbjct: 405 KISDRIMEMEKSVKENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSK 464

Query: 421 RVEMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 452
           +VEM SI P+G+ SM ESR+GNGG+E+G++LP+  +N FSSLFY+ ++
Sbjct: 465 KVEMVSINPNGSFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVK 500

BLAST of MC04g1963 vs. NCBI nr
Match: XP_022943466.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like, partial [Cucurbita moschata])

HSP 1 Score: 489 bits (1260), Expect = 1.11e-167
Identity = 271/467 (58.03%), Postives = 330/467 (70.66%), Query Frame = 0

Query: 1   QAMAPLEILEVCKISPS-SPAADFSLPLTFFDLFFLASSPTERILYYHH------HFDSS 60
           Q  A +E+LEVC I+PS SP   FSLPLTF DL    S PTERIL+Y         FDS 
Sbjct: 4   QPKAIMEVLEVCNITPSTSPPTHFSLPLTFSDLVQFISPPTERILFYSLPANDLLRFDS- 63

Query: 61  SVLPKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSAD 120
            +L  LK SLSL LSHFLPLAGNLVWP  S +P ILY PGD+VSLTIA++DADF  L ++
Sbjct: 64  -ILQTLKHSLSLTLSHFLPLAGNLVWPHHSQDPFILYNPGDSVSLTIAKTDADFDHLLSN 123

Query: 121 HVRKATDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFL 180
           HVRKA +SHFLVPQL +SD ++ P M LQITLFPKS  GFSIGI+T+HVVVDGKTST+FL
Sbjct: 124 HVRKAKESHFLVPQLPTSD-TIAPPMSLQITLFPKS--GFSIGIITSHVVVDGKTSTMFL 183

Query: 181 KSWASICKLC------LSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGS 240
           KSWASIC         L +LPPELTP FDRTA +DP  +G +  Y K  G F+       
Sbjct: 184 KSWASICSTLNCTNNNLPTLPPELTPFFDRTAAKDP--NGLERLYVKHYGAFVRQPKI-- 243

Query: 241 RKLRLTPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRI 300
             L LTPK  ISDD+V  TF +TR  +E LRRK   +++STR HLTTFVL  A +S C +
Sbjct: 244 --LVLTPKRVISDDVVHATFELTRIDIEKLRRKVATNSTSTR-HLTTFVLTYALISTCIV 303

Query: 301 KAERLEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYGFLTEAREFEEENAMEIIQSK 360
           KAER+EPKSK+ LFF VD R R GA  G NYFGNGV G G   EA+E EEEN M ++ +K
Sbjct: 304 KAERIEPKSKIALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVANK 363

Query: 361 ISDAIAEVTE--EKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKR 420
           ISD I E+ +  ++N+  EMV E+  R  +EMP  + I+A GSPRFGVYE DFGWG  K+
Sbjct: 364 ISDRIMEMEKSVKENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSKK 423

Query: 421 VEMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 452
           VEM SI P+G+ SM ESR+GNGG+E+G++LP+  +N FSSLFY+ ++
Sbjct: 424 VEMVSINPNGSFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVK 458

BLAST of MC04g1963 vs. ExPASy TrEMBL
Match: A0A6J1J6U6 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111483993 PE=3 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 3.06e-170
Identity = 272/466 (58.37%), Postives = 335/466 (71.89%), Query Frame = 0

Query: 1   QAMAPLEILEVCKISPS-SPAADFSLPLTFFDLFFLASSPTERILYYHHHFDS----SSV 60
           Q  A +E+LEVC I+PS SP   FSLPLTF DL    S PTERIL+Y  H +      S+
Sbjct: 5   QPKAIMEVLEVCNITPSTSPPTHFSLPLTFSDLVQFLSPPTERILFYTLHANHLPRFDSI 64

Query: 61  LPKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADHV 120
           L  LK SLSL LSHFLPLAGNLVWP  SP+P ILY  GD+VSLTIA+++ADF  LS++HV
Sbjct: 65  LQTLKHSLSLTLSHFLPLAGNLVWPHHSPDPFILYNHGDSVSLTIAKTEADFHHLSSNHV 124

Query: 121 RKATDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKS 180
           RKAT+SHFLVPQL +SD ++ PAM LQITLFPKS  GFSIGI+TNHVVVDGKTST+FLKS
Sbjct: 125 RKATESHFLVPQLPTSD-TIAPAMSLQITLFPKS--GFSIGIITNHVVVDGKTSTMFLKS 184

Query: 181 WASICKLC-------LSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSR 240
           WASIC          L +LP ELTP FDRTA +DP  +G Q  Y K  G F+        
Sbjct: 185 WASICSTLNCTNNNNLPTLPSELTPFFDRTAAKDP--NGLQRLYVKHYGAFVRQPKI--- 244

Query: 241 KLRLTPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRIK 300
            L LTPK  ISDD+V  TF +TR  +E LRRK   +++STR HLTTFVL  A VS C ++
Sbjct: 245 -LGLTPKRVISDDVVYATFELTRIDIEKLRRKVATNSTSTR-HLTTFVLTYALVSTCIVQ 304

Query: 301 AERLEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYGFLTEAREFEEENAMEIIQSKI 360
           AER+EPKSK+ LFF VD R R GA  G NYFGNGV G G + EA+E EEEN M ++ +KI
Sbjct: 305 AERIEPKSKIALFFAVDMRPRMGALGGLNYFGNGVIGLGEILEAKELEEENGMAMVANKI 364

Query: 361 SDAIAEVTE--EKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKRV 420
           SD I E+ +  ++N+  EMV E++ R  +EMP  + I+A GSPRFGVYE DFGWG  K+V
Sbjct: 365 SDRIMEMEKSVKENKVVEMVEEISDRWGREMPVSKMIVAGGSPRFGVYEIDFGWGNSKKV 424

Query: 421 EMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 452
           EM+S+ P+G+ SM ESR+GNGG+E+G++LP+  +N FSSLFY+ ++
Sbjct: 425 EMASMDPNGSFSMTESRSGNGGVEVGVVLPKDVMNVFSSLFYEEVK 460

BLAST of MC04g1963 vs. ExPASy TrEMBL
Match: A0A6J1FRS6 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111448225 PE=3 SV=1)

HSP 1 Score: 489 bits (1260), Expect = 5.37e-168
Identity = 271/467 (58.03%), Postives = 330/467 (70.66%), Query Frame = 0

Query: 1   QAMAPLEILEVCKISPS-SPAADFSLPLTFFDLFFLASSPTERILYYHH------HFDSS 60
           Q  A +E+LEVC I+PS SP   FSLPLTF DL    S PTERIL+Y         FDS 
Sbjct: 4   QPKAIMEVLEVCNITPSTSPPTHFSLPLTFSDLVQFISPPTERILFYSLPANDLLRFDS- 63

Query: 61  SVLPKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSAD 120
            +L  LK SLSL LSHFLPLAGNLVWP  S +P ILY PGD+VSLTIA++DADF  L ++
Sbjct: 64  -ILQTLKHSLSLTLSHFLPLAGNLVWPHHSQDPFILYNPGDSVSLTIAKTDADFDHLLSN 123

Query: 121 HVRKATDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFL 180
           HVRKA +SHFLVPQL +SD ++ P M LQITLFPKS  GFSIGI+T+HVVVDGKTST+FL
Sbjct: 124 HVRKAKESHFLVPQLPTSD-TIAPPMSLQITLFPKS--GFSIGIITSHVVVDGKTSTMFL 183

Query: 181 KSWASICKLC------LSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGS 240
           KSWASIC         L +LPPELTP FDRTA +DP  +G +  Y K  G F+       
Sbjct: 184 KSWASICSTLNCTNNNLPTLPPELTPFFDRTAAKDP--NGLERLYVKHYGAFVRQPKI-- 243

Query: 241 RKLRLTPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRI 300
             L LTPK  ISDD+V  TF +TR  +E LRRK   +++STR HLTTFVL  A +S C +
Sbjct: 244 --LVLTPKRVISDDVVHATFELTRIDIEKLRRKVATNSTSTR-HLTTFVLTYALISTCIV 303

Query: 301 KAERLEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYGFLTEAREFEEENAMEIIQSK 360
           KAER+EPKSK+ LFF VD R R GA  G NYFGNGV G G   EA+E EEEN M ++ +K
Sbjct: 304 KAERIEPKSKIALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVANK 363

Query: 361 ISDAIAEVTE--EKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKR 420
           ISD I E+ +  ++N+  EMV E+  R  +EMP  + I+A GSPRFGVYE DFGWG  K+
Sbjct: 364 ISDRIMEMEKSVKENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSKK 423

Query: 421 VEMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 452
           VEM SI P+G+ SM ESR+GNGG+E+G++LP+  +N FSSLFY+ ++
Sbjct: 424 VEMVSINPNGSFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVK 458

BLAST of MC04g1963 vs. ExPASy TrEMBL
Match: A0A6J1D383 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111017189 PE=3 SV=1)

HSP 1 Score: 488 bits (1257), Expect = 1.82e-167
Identity = 277/470 (58.94%), Postives = 333/470 (70.85%), Query Frame = 0

Query: 1   QAMAPLEILEVCKISPS---SPAAD-FSLPLTFFDLFFLASSPTERILYYHHHFDSSSVL 60
           Q    L+ILE+CKISPS   SPAAD FSLPLTFFDL F  S PTERIL+Y H   + S+L
Sbjct: 3   QPKPSLKILELCKISPSPSPSPAADQFSLPLTFFDLPFFTSPPTERILFYSHP-AADSIL 62

Query: 61  PKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSD-ADFSRLSADHV 120
            KLK SLSL LSHFLPLAGN+VWP+ SPEP ILYKPGD+VSLT+AQ+D ADF RLS+D +
Sbjct: 63  QKLKHSLSLTLSHFLPLAGNVVWPEDSPEPFILYKPGDSVSLTVAQADGADFHRLSSDQI 122

Query: 121 RKATDSHFLVPQLYSSDDSVF--PAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFL 180
           RKA +SHFLVPQ  + DD+    P + LQITLFP    GFSIGI TNH V DGKTST FL
Sbjct: 123 RKANESHFLVPQFQAPDDTTCAAPILCLQITLFP--GVGFSIGIATNHTVFDGKTSTTFL 182

Query: 181 KSWASIC-----KLCLSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSR 240
           KSWASIC         S+LP   T DFDR A +DP   G Q  Y K+  IFLSP  T  +
Sbjct: 183 KSWASICTHESESESESNLP---TLDFDRAAAKDP--DGLQKLYLKYHQIFLSPPDTSGQ 242

Query: 241 KLRLTPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRH-LTTFVLALAHVSICRI 300
           +LRL PKVAI DD+  GTF +T + VE+LR  A  S+SSTRR  L+TFVL  AHVSIC +
Sbjct: 243 RLRLAPKVAIPDDVAGGTFELTCADVEHLRATAAMSSSSTRRRRLSTFVLTYAHVSICIV 302

Query: 301 KAERLEPKSKVGLFFPVDWRLRAGAP-VGPNYFGNGVCGYGFLTEAREFEEENAMEIIQS 360
           +A+RL+PK+KV LFFPVDWR R G   VG NYFGNG+ G G   EA++F  EN + +I +
Sbjct: 303 RAQRLDPKTKVALFFPVDWRPRLGGRLVGENYFGNGIGGCGLFLEAKDFFGENRIGVISN 362

Query: 361 KISDAIAEVTEEKNRADEMVVELAARLM----KEMPFCRTIMAAGSPRFGVYETDFGWGR 420
           +IS+ I E+ EEK +    VVEL   +      +MP  + I  AGSPRFG+Y+ DFGWG 
Sbjct: 363 QISEVIMEM-EEKVKGSTEVVELMDHVFLQWFSKMPVDKRITVAGSPRFGLYDLDFGWGG 422

Query: 421 CKRVEMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 452
           C +VEM+S+  DGT SM ESRNGNGG E+GI+LPQ  +N FSSLF++GL+
Sbjct: 423 CTKVEMTSMGLDGTFSMAESRNGNGGAEVGIVLPQDDMNLFSSLFFEGLK 463

BLAST of MC04g1963 vs. ExPASy TrEMBL
Match: A0A6J1FXM3 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111448598 PE=3 SV=1)

HSP 1 Score: 481 bits (1239), Expect = 1.32e-164
Identity = 266/467 (56.96%), Postives = 326/467 (69.81%), Query Frame = 0

Query: 1   QAMAPLEILEVCKISPS-SPAADFSLPLTFFDLFFLASSPTERILYYH------HHFDSS 60
           Q  A +E+LEVC I+PS SP   FSLPLTFFDL    + PTERIL+Y         FDS 
Sbjct: 17  QQKAIMEVLEVCNITPSPSPPTHFSLPLTFFDLVQFLAPPTERILFYTLPANDLPRFDS- 76

Query: 61  SVLPKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSAD 120
            +L  LK SLSL LSHFLPLAGNLVWP  S +P ILY PGD+VSLTIA+++ADF  LS++
Sbjct: 77  -ILQTLKHSLSLTLSHFLPLAGNLVWPHHSQDPFILYNPGDSVSLTIAKTEADFHHLSSN 136

Query: 121 HVRKATDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFL 180
           HVRKA +SHFLVPQL +SD ++ P M LQITLFPKS  GFSIGIVTNHVVVDGKTST+FL
Sbjct: 137 HVRKAKESHFLVPQLPTSD-TIAPPMSLQITLFPKS--GFSIGIVTNHVVVDGKTSTMFL 196

Query: 181 KSWASICKLC------LSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGS 240
           KSWASIC         L +LPPELTP FDRTA RDP  +G Q  Y K+ G F+       
Sbjct: 197 KSWASICSTLNCTNNNLPTLPPELTPFFDRTAARDP--NGLQRLYVKYYGAFVRQPKI-- 256

Query: 241 RKLRLTPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRI 300
             L LTPK  ISDD+V  TF +TR+ +E LR K   ++SST R L+TF+LA + +S C +
Sbjct: 257 --LGLTPKRVISDDVVYATFELTRTDIEKLRTKVVATSSSTPRRLSTFMLAFSLISTCIV 316

Query: 301 KAERLEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYGFLTEAREFEEENAMEIIQSK 360
           KAER+EPKSK+ LFF +D R R GA  G NYFGN V   G   EA+E EEEN M ++ +K
Sbjct: 317 KAERIEPKSKIALFFAMDMRPRMGALGGLNYFGNCVSTLGVFLEAKELEEENGMAMVANK 376

Query: 361 ISDAIAEVTE--EKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKR 420
           ISD + E+    ++N+  EMV E+  R  +EMP  + I   GSPRFGVYE DFGWG  K+
Sbjct: 377 ISDRMMEIENSVKENKVVEMVEEILGRWGREMPISKMIGVGGSPRFGVYEIDFGWGNSKK 436

Query: 421 VEMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 452
           VEM+S+ P+GT S+ ESR+GNGG+E+GI LP   +N  SSLFY+ ++
Sbjct: 437 VEMASMDPNGTFSITESRSGNGGVEVGIALPNDVMNVLSSLFYEEVK 472

BLAST of MC04g1963 vs. ExPASy TrEMBL
Match: A0A6J1FVL4 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111448866 PE=3 SV=1)

HSP 1 Score: 471 bits (1213), Expect = 1.00e-160
Identity = 257/461 (55.75%), Postives = 319/461 (69.20%), Query Frame = 0

Query: 6   LEILEVCKISPS-SPAADFSLPLTFFDLFFLASSPTERILYY----HHHFDSSSVLPKLK 65
           LEILE CKI PS SP   FSLPLT+FDL F  S PT+RIL+Y    H      S+L  LK
Sbjct: 14  LEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYSILQTLK 73

Query: 66  RSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADHVRKATD 125
            SLS ALSHFLPLAGNLVWP QSPEP I Y PGD++SLTIA++ ADF  LS++H RKA +
Sbjct: 74  HSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARKAKE 133

Query: 126 SHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKSWASIC 185
           SHFLVP L +SD ++ P M LQITLFPK   GFSIGI+TNHVV D KTST+FLKSWASIC
Sbjct: 134 SHFLVPDLPTSD-TIAPVMSLQITLFPKC--GFSIGIITNHVVSDAKTSTMFLKSWASIC 193

Query: 186 KLCLSS-------LPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSRKLRLT 245
               ++       LP ELTP FDRT   DP  +G  T Y K   IF+       + L LT
Sbjct: 194 STLNNTNNKNPPTLPSELTPCFDRTPATDP--NGLHTLYVKSFAIFVP----NPKLLGLT 253

Query: 246 PKVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRIKAERLE 305
           PK  ISDD+V  TF +TR  +E LRR+   ++SST R L+TF+LA + VS C +KA+R E
Sbjct: 254 PKEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTE 313

Query: 306 PKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYGFLTEAREFEEENAMEIIQSKISDAIA 365
           P+SK+GL F VDWR R       NYFGNGV GYG   EARE E EN M +I +KISDA+A
Sbjct: 314 PESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALA 373

Query: 366 EVTE--EKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKRVEMSSI 425
           E+ +  ++N+  EM+ E++ R  K MP  + ++ AGSPR GVY+ DFGWGR K+VE++SI
Sbjct: 374 EIEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASI 433

Query: 426 TPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 452
           +P+G  SM ESRNG+GG+E+GI LP  A++ F SLF + ++
Sbjct: 434 SPNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMK 465

BLAST of MC04g1963 vs. TAIR 10
Match: AT5G39080.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 286.2 bits (731), Expect = 4.6e-77
Identity = 182/468 (38.89%), Postives = 259/468 (55.34%), Query Frame = 0

Query: 3   MAPLEILEVCKISPSS--PAADFSLPLTFFDLFFLASSPTERILYYH-----HHFDSSSV 62
           ++ L I+EV +++PS+   A   SLPLT+FDL +      ER+++Y        F  S +
Sbjct: 2   VSSLNIIEVARVTPSNSDSAESLSLPLTYFDLIYYKLRAVERVIFYRITNVTRPFFDSVI 61

Query: 63  LPKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADHV 122
           +P LK SLS  LSH+LPLAG L+W     +P I+Y   D VS ++A+++ADFS LS +  
Sbjct: 62  VPNLKTSLSSCLSHYLPLAGKLIWEPLDHKPTIVYSQNDDVSFSVAETNADFSSLSGNEP 121

Query: 123 RKATDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKS 182
             +T+ + LVP L SSDDS    +  Q+TLFP    GF IG+  +H V+DGKT+T+FLKS
Sbjct: 122 FPSTELYPLVPALQSSDDSA-SIVSFQVTLFPNQ--GFCIGVSAHHAVLDGKTTTMFLKS 181

Query: 183 WASICKLCLSSLPPELTPDFDRTAIRDPVGS------GWQTAYEKFLGIFLSPTSTGSRK 242
           WA ICK    SLP +L P +DRT I+ P  S       W+ ++ K L     P +   + 
Sbjct: 182 WAHICKHQDFSLPQDLIPTYDRTVIKSPTDSENKVLNEWR-SFTKILAGGKEPAN--PKS 241

Query: 243 LRLTPKVAISDDLVTGTFHVTRSGV----ENLRRKATNSNSST----RRHLTTFVLALAH 302
           L+L P   I  D+V  T  +TR  +    E L+R+ ++S+SST       L+TFV+  ++
Sbjct: 242 LKLNPSFEIGPDVVRYTLQLTREDIQTLRERLKREVSSSSSSTSSSKELRLSTFVIVYSY 301

Query: 303 VSICRIKAERLEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYG-FLTEAREFEEENA 362
           V +C I+A   EP   VG  F VD R     P  PNYFGN + G    +  A+ F  E  
Sbjct: 302 VLVCIIRARGGEPHRPVGYAFSVDCRSLMNPPT-PNYFGNCIAGCSRMMLTAKMFMGEEG 361

Query: 363 MEIIQSKISDAIAEVTEEKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGW 422
           +    + +SD+I E  E         V  A       P  + I+ +GS RFGVYE DFGW
Sbjct: 362 LLAAATMVSDSIEEWDESFAWKIPDFVAYATL----PPETQLILVSGSNRFGVYELDFGW 421

Query: 423 GRCKRVEMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFY 449
           GR  +V + SI+P   +SM ESR+ NG +EIG  L +H ++    L +
Sbjct: 422 GRPDKVMVVSISPGNGISMAESRDQNGSVEIGFSLKKHEMDTLIDLLH 458

BLAST of MC04g1963 vs. TAIR 10
Match: AT3G29590.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 284.3 bits (726), Expect = 1.8e-76
Identity = 174/458 (37.99%), Postives = 257/458 (56.11%), Query Frame = 0

Query: 6   LEILEVCKISPSSPAADFSLPLTFFDLFFLASSPTERILYYH-HHFDSSSVLPKLKRSLS 65
           + ILEV ++SP S +   +LPLT+FDL +L   P +R+L+YH      SS++ KLK SLS
Sbjct: 8   VNILEVVQVSPPS-SNSLTLPLTYFDLGWLKLHPVDRVLFYHVPELTRSSLISKLKSSLS 67

Query: 66  LALSHFLPLAGNLVWPDQSPEPIILYKPG--DAVSLTIAQSDADFSRLSADHVRKATDSH 125
             L H+LPLAG LVW     +P I+Y P   DAV LT+A+S+ D S LS D  R AT+ H
Sbjct: 68  ATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEPRPATEFH 127

Query: 126 FLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKSWASICKL 185
            LVP+L  SD+S    + +Q+T FP    GFS+G+  +H V+DGKT+ +FLK+WA  CK 
Sbjct: 128 SLVPELPVSDESA-RVLAVQVTFFPNQ--GFSLGVTAHHAVLDGKTTAMFLKAWAHNCKQ 187

Query: 186 CLSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSRKLRLTPKVAISDDL 245
              +LP +L P  DR  ++DP G        K L  ++S  S     L+L P   I  D+
Sbjct: 188 EQEALPHDLVPSLDRIIVQDPTG-----LETKLLNRWIS-ASNNKPSLKLFPSKIIGSDI 247

Query: 246 VTGTFHVTRSGVENLRRKATNSNSSTRRHLTTFVLALAHVSICRIKAERLEPKSKVGLFF 305
           +  T+ +TR  ++ LR +    + + +  L+TFV+  A+V  C +K    +P   V + F
Sbjct: 248 LRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTRFVCVGF 307

Query: 306 PVDWRLRAGAPVGPNYFGNGVCGYG-FLTEAREFEEE-------NAMEIIQSKISDAIAE 365
             D+R R   P+ P +FGN + G G F  +A    EE        A+E +   ++    E
Sbjct: 308 ASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFITAVETLTGWVNGLCPE 367

Query: 366 VTEEKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKRVEMSSITPD 425
             E+      M++   A    E P  + I  AGS R G+Y +DFGWG+  +VE+ +I  D
Sbjct: 368 NIEK-----NMLLPFEAFKRME-PGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTIDKD 427

Query: 426 GTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 453
            ++S+ ES +G+GG+E+G+ L +  +  F SLF  GLE
Sbjct: 428 ASVSLSESGDGSGGVEVGVCLKKDDVERFGSLFSIGLE 449

BLAST of MC04g1963 vs. TAIR 10
Match: AT5G39050.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 280.4 bits (716), Expect = 2.5e-75
Identity = 175/468 (37.39%), Postives = 254/468 (54.27%), Query Frame = 0

Query: 6   LEILEVCKISPSS--PAADFSLPLTFFDLFFLASSPTERILYY-----HHHFDSSSVLPK 65
           L++++V +++PS+   +   +LPLTFFDL +      ER+++Y        F  S ++P 
Sbjct: 10  LKVIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIVPN 69

Query: 66  LKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADHVRKA 125
           LK SLS +LSH+LPLAG LVW    P+P I+Y P DAVS T+A+S+ADFSRL+       
Sbjct: 70  LKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKEPFPT 129

Query: 126 TDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKSWAS 185
           T+ + LVP+L+ SDDS   A+  Q+TLFP    GF I +  +H V+DGKT+T FLKSWA 
Sbjct: 130 TELYPLVPELHVSDDSA-SAVSFQVTLFPNQ--GFCISVNAHHAVLDGKTTTNFLKSWAR 189

Query: 186 ICKLCLSSLPPELTPDFDRTAIRDPVG------SGWQTAYEKFLGIFLSPTSTGSRKLRL 245
            CK   S LP +L P +DRT I+DP+       + W    + F G    P +  S KL  
Sbjct: 190 TCKNQDSFLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTG-GKEPENPKSLKLLW 249

Query: 246 TPKVAISDDLVTGTFHVTRSGVENLRRKATNSNSST-------RRHLTTFVLALAHVSIC 305
           +P+  I  D+   T ++TR  ++ LR +    +SS+          L+TFV+  ++   C
Sbjct: 250 SPE--IGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYALTC 309

Query: 306 RIKAERLEPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCG-YGFLTEAREFEEENAMEII 365
            IKA   +P   VG  F VD R     PV  +YFGN V   +     A  F  E      
Sbjct: 310 LIKARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFLAA 369

Query: 366 QSKISDAIAEVTEEKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCK 425
              +SD++  + E        + E+        P  + +  AGS RFGVY  DFGWGR +
Sbjct: 370 ARMVSDSVEALDE---NVALKIPEILEGFTTLSPGTQVLSVAGSTRFGVYGLDFGWGRPE 429

Query: 426 RVEMSSITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 453
           +V + SI     +S  ESR+G+GG+E+G  L +H ++    L ++GLE
Sbjct: 430 KVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLE 468

BLAST of MC04g1963 vs. TAIR 10
Match: AT3G29635.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 276.2 bits (705), Expect = 4.8e-74
Identity = 185/462 (40.04%), Postives = 253/462 (54.76%), Query Frame = 0

Query: 9   LEVCKISPSSPAADFS--------LPLTFFDLFFLASSPTERILYYHHHFDS------SS 68
           L+V KIS  SPA++ S        LPLTFFDL +L   PTER+++Y    DS      S 
Sbjct: 3   LKVTKISQVSPASNSSNDSANSMVLPLTFFDLRWLQFHPTERVIFYKLIKDSSLESFLSV 62

Query: 69  VLPKLKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADH 128
           +LPKL+ SLS+ L H+LPLAG L W  Q P+P I+  P D VSLT+A+SDADFSR+S   
Sbjct: 63  ILPKLELSLSIVLRHYLPLAGRLTWSSQDPKPSIIVSPNDYVSLTVAESDADFSRISGKG 122

Query: 129 VRKATDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLK 188
           +R  ++   LVP+L  S DS    + LQ+TLFP    GF IGI ++H V+DGKT   F+K
Sbjct: 123 IRPESEIRSLVPELSLSCDSP-SVLSLQVTLFPNQ--GFCIGIASHHSVMDGKTVVRFIK 182

Query: 189 SWASICKLCLSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSRKLRLTP 248
           SWA ICK     LP +LTP  DRT I  P  +       + L  F S      R L+L P
Sbjct: 183 SWAHICKHGAMDLPEDLTPVLDRTVINVP--ASLDAKIIELLSYF-SEVKDSFRSLKLLP 242

Query: 249 KVAISDDLVTGTFHVTRSGVENLRRKATNSNSSTRR--HLTTFVLALAHVSICRIKAERL 308
              IS DLV  +  +TR  +E LR +A   ++ +    HL+TFV+A A++  C +K    
Sbjct: 243 PKEISPDLVRISLELTRENIEKLREQAKRESARSHHELHLSTFVVANAYLWTCLVKTRGG 302

Query: 309 EPKSKVGLFFPVDWRLRAGAPVGPNYFGNGVCGYG-FLTEAREFEEENAMEIIQSKISDA 368
           +    V   +  D+R R   PV   YFGN V   G F  +A  F  E+    +   +SD+
Sbjct: 303 DENRPVRFMYAADFRNRLDPPVPEMYFGNCVFPIGCFGYKANVFLGEDGFVNMVEILSDS 362

Query: 369 IAEVTEEKNRADEMVVELAARLMKEM-PFCRTIMAAGSPRFGVYETDFGWGRCKRVEMSS 428
           +  +   K    E + EL     K + P  +    AGS +FG+Y +DFGWG+    E++S
Sbjct: 363 VRSIGLRKL---ETICELYINGTKSVKPGTQIGSIAGSNQFGLYGSDFGWGKPCNSEIAS 422

Query: 429 ITPDGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 453
           I  +   SM E R+  GG+EIG+ L +  ++ F  LF  GLE
Sbjct: 423 IDRNEAFSMSERRDEPGGVEIGLCLKKCEMDIFIYLFQNGLE 455

BLAST of MC04g1963 vs. TAIR 10
Match: AT5G39090.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 275.8 bits (704), Expect = 6.2e-74
Identity = 177/459 (38.56%), Postives = 258/459 (56.21%), Query Frame = 0

Query: 6   LEILEVCKISP--SSPAADFSLPLTFFDLFFLASSPTERILYY-----HHHFDSSSVLPK 65
           L  + V +++P  S+ +A  +LPLTFFDL +L     ER+++Y     +     S ++P 
Sbjct: 5   LNFIHVSRVTPSNSNSSASLTLPLTFFDLLWLKHKAVERVIFYKLTDVNRSLFDSVIVPN 64

Query: 66  LKRSLSLALSHFLPLAGNLVWPDQSPEPIILYKPGDAVSLTIAQSDADFSRLSADHVRKA 125
           LK SLS +LSH+LPLAG+++W    P+P I+Y   DAVS T+A+S++DFS L+      +
Sbjct: 65  LKSSLSSSLSHYLPLAGHIIWEPHDPKPKIVYTQNDAVSFTVAESNSDFSLLTGKEPFSS 124

Query: 126 TDSHFLVPQLYSSDDSVFPAMRLQITLFPKSSGGFSIGIVTNHVVVDGKTSTLFLKSWAS 185
           T+ H LVP+L +SDDS    +  Q+TLFP    GF IG+ T+H V DGKT+T FLKSWA 
Sbjct: 125 TELHPLVPELQNSDDSA-AVVSFQVTLFPNQ--GFCIGVTTHHAVSDGKTTTTFLKSWAH 184

Query: 186 ICKLCLSSLPPELTPDFDRTAIRDPVGSGWQTAYEKFLGIFLSPTSTGSRKLRLTPKVAI 245
           +CK   SSLP +L P +DRT I+ P          K L I+ S      + L+L P+  I
Sbjct: 185 LCKHQDSSLPDDLIPFYDRTVIKGP-----PEIDTKVLKIWHSIHK--PKSLKLLPRPEI 244

Query: 246 SDDLVTGTFHVTRSGVENLRRKATNSNSS-TRRHLTTFVLALAHVSICRIKAERLEPKSK 305
             D+V  TF +TR  +E LR K    +SS +   L+TFV+  ++V  C I +   +P   
Sbjct: 245 ESDVVRYTFELTRENIEKLRDKLKRESSSFSSVRLSTFVITFSYVFTCLIGSGGDDPNRP 304

Query: 306 VGLFFPVDW-RLRAGAPVGPNYFGNGVCGYGFL---TEAREFEEENAMEIIQSKISDAIA 365
           VG  F VD  RL    P+   YFGN  C Y  +    +A  F  E    +    ISD++ 
Sbjct: 305 VGYRFAVDCRRLIDDPPIPLTYFGN--CVYSAVKIPLDAGMFLGEQGFVVAARLISDSVE 364

Query: 366 EVTEEKNRADEMVVELAARLMKEMPFCRTIMAAGSPRFGVYETDFGWGRCKRVEMSSITP 425
           E+  + N A + + EL     K     + +  AGS RFG+Y  DFGWG+  +  + SI  
Sbjct: 365 EL--DSNVAWK-IPELLETYEKAPVDSQFVSVAGSTRFGIYGLDFGWGKPFKSLLVSIDQ 424

Query: 426 DGTLSMVESRNGNGGLEIGILLPQHAINDFSSLFYQGLE 453
            G +S+ ESR+G+GG+EIG  L +  +N    L ++G++
Sbjct: 425 RGKISIAESRDGSGGVEIGFSLKKQEMNVLIDLLHKGIK 448