MC04g1908 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g1908
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like
LocationMC04: 26077953 .. 26080966 (+)
RNA-Seq ExpressionMC04g1908
SyntenyMC04g1908
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATTTTTTTAAAATAAGAAGATGGACGTTGACTAATAAACATATATTTATGTTATTTTGTAATAACCGATTTGGGGAAATTGATATGGTTTGCGGTGAATATGGGACATGAAATTTGAGGTGCCACGTGGCATACCAGCAACCTTAGTTGTGTGGTCGTTAACAATTTGAAAGGCTATCCACTGGCGACATCGATTTTTGAACACTCGAAAGTTCCAAATGTGGTGTCCCCCTTCATAAGCTACAACTTCATACTTGAAACTGTGCACACTCCAAACTTTACTCCTTCCACTCAATTGCCTTATTTTCTCTCCGACCTCTGAAACCCATGGCCACCGCTATCTCCACCGCCGGAGCCGTCAACAGGATTCCGGTAATTCAAATCTTTCCCGCCATTACAACAATATTCCACTCGAACCTTGATAGTTTTCTTTCTTTCTTTCTTTCTTTCTTCTCTGTTGTTCAATTAGAATTGTTGGTCTTCTGGGTTTGTAGAGATCATGGGAAAAATTTAAACTTTTTACCTTCTCCTCAACTCATGTTCAACTGTTTGTTACCAACGATATAAACGGTGTTTACTGACAAATCGTTCTAATGCTTGGTTTCACACTTTTGAAAGTGATTTTGGTCATGTCTTTAGAGTTAGAGTTTGTGAATTTATGGTCACAAGATGTGGAATTAGAGAGGTGAAGAATGGTAAATTGGGTGAATGAAAGCGTGGGGGATTGTTTATTGCAGGTGAGTTTAAATGGCGCCATGTCTGGAGCTTCAGTTCAGAGCTCTGCATTCTTCGGCAGCAGCTTGAAGAAAGTGAACTCAAAATCATACCCCAATTCAAAGATCTCCTCAGGAAGCAGCTTTAAGGTAGTTGCTGTGGCAGAGGAAATTGATGAAAAGAAGCAAACTGAAAAGGATAGATGGAAGGGTCTTGCTTTTGATGTCTCTGATGATCAGCAAGACATCACCAGAGGAAAGGGAATGGTGGATTCACTCTTCCAAGCCCCCACAGGAGCAGGAACTCATGACCCTGTTCTGAGTTCTTATGAATATTTAAGCGCAGGACTTCGTCAGTAAGTCTAAACAAAACAAAGTTCAGATTTTTCTTTTCTTTTTCTTTTCGGTCTCTGGTTTTCATTGTAGTAATTAAGGAAGGAACTGATGGTCTAGAATGTTGTGGTTTTGATGATGATATAGGTACAATTTAGACAACAGCGTGGATGGCTTTTACATTGCTCCTGCCTTCATGGACAAACTAGTTGTTCACATCACTAAGAACTTCATGAAACTTCCCAACATCAAGGTTCGACAGAAAACTGATTTACTGTTTATTAGCTTCATGCTGGATTTGGCTAGAGTTTTCTTTAGCTTATTGATTGATCTAATCCTTCTGCCTTAGGTTCCTCTTATTTTGGGTGTTTGGGGAGGCAAAGGTCAAGGAAAATCTTTCCAATGTGAACTTGTCTTTGCCAAGATGGGAATCAAGTGAGTTTCATCCTTCTCCATCCTGTTTTTTTCAGCCATTTCAGATCATTTTCTTGATTGTGATGTGGTGCTTTCAGTGTTCTACACATTTGTATTTCTTCACCCTGCTGCTAACGCTCTGTTTCATTACTTCAGCCCCATTATGATGAGTGCCGGAGAATTGGAGAGTGGAAATGCTGGAGAACCTGCAAAATTGATTCGACAGAGGTACCGTGAAGCGGCAGACATTATCAAGAAGGGAAAGATGTGCGTTCTCTTCATCAACGATCTTGATGCCGGAGCTGGTCGTCTCGGTGGAACCACCCAATACACTGTGAACAACCAGATGGTTAACGCTACCCTCATGAACATAGCTGATAACCCTACTAATGTCCAGCTCCCTGGTATGTACAACAAAGAGGACAATCCTCGTGTTCCCATTGTTGTCACGGGTAACGACTTCTCGACATTGTATGCTCCTCTCATCCGTGATGGTCGTATGGAAAAATTCTACTGGGCACCCACACGAGATGATCGAATTGGTGTCTGCTCAGGAATCTTTAGCACTGACAATGTATCAAAAGAAGACATAATCAAAATTGTTGATACATTCCCTGGCCAATCCATTGGTAAGCGTCAAAGTGTAAACTACGAATCAAATACATAAATATTTTGGTTCATTTCCCTTCTATGTTCCAACCTGATAAACTAAATCATGATCATACAGATTTCTTTGGTGCCCTGAGGGCAAGAATATACGATGATGAAGTGAGGAAGTGGGTTGTTTCCGTTGGAGTTGATAATATCGGGAAGAAGCTCGTCAACTCAAAGGATCCACCGCCGAAATTCGAGCAGCCAAAGATAACATTGAAGAAGCTCCTCGAGTATGGAAACATGCTTGTTGAGGAGCAAGAGAACGTGAAGAGAGTCCAGTTGGCTGACAAGTACTTGAGCGATGCTGCTCTTGGTGATGCCAATGAAGATGCTATAAAAAGTGGAGCATTCTACGGTTAGATCTCTCTCTTGATTCTTTTATAGACCTTTAAGAATTGCACAAAAGCAGATTCTCCTAAGCTGTTGCTTCTGTTTGTTGCAGGGAAAGCCGCCCAGCAGGTGCATATTCCTGTCCCTGAAGGTTGCACAGACCCAAATGCATCAAACTTTGACCCAACTGCTAGGAGTGATGATGGAAGCTGCAGTTATCAGTTATAAGCCTTCCGCCAATAGCTGTTCGAGTGGAAAAAGATCACCTTCTACTCATGCTATACAAGGAGAATACTGTCACTGACTCACCTGTTTGAATCAAAATAGCCACTGGTTTGCTTTACTGTATGCTTCAGAGTTTGTGGGGGGTTTCAGTTCTCATTTCTTTTTCCCTTTTTTCCCTTGTGTTGCCACGACATTGAGATATCTTTTGTGAGTGTCCATAAAGGACGTAACCTGGCGTCTCATAGATTTTTGTGATATTGAGATATTTTCTTCAGTCTTGATTATACCATACATCCACAAAATGAATGCAGAATCATTAAGGATTGAGAACCTTGAAGACAAAC

mRNA sequence

TTATTTTTTTAAAATAAGAAGATGGACGTTGACTAATAAACATATATTTATGTTATTTTGTAATAACCGATTTGGGGAAATTGATATGGTTTGCGGTGAATATGGGACATGAAATTTGAGGTGCCACGTGGCATACCAGCAACCTTAGTTGTGTGGTCGTTAACAATTTGAAAGGCTATCCACTGGCGACATCGATTTTTGAACACTCGAAAGTTCCAAATGTGGTGTCCCCCTTCATAAGCTACAACTTCATACTTGAAACTGTGCACACTCCAAACTTTACTCCTTCCACTCAATTGCCTTATTTTCTCTCCGACCTCTGAAACCCATGGCCACCGCTATCTCCACCGCCGGAGCCGTCAACAGGATTCCGGTGAGTTTAAATGGCGCCATGTCTGGAGCTTCAGTTCAGAGCTCTGCATTCTTCGGCAGCAGCTTGAAGAAAGTGAACTCAAAATCATACCCCAATTCAAAGATCTCCTCAGGAAGCAGCTTTAAGGTAGTTGCTGTGGCAGAGGAAATTGATGAAAAGAAGCAAACTGAAAAGGATAGATGGAAGGGTCTTGCTTTTGATGTCTCTGATGATCAGCAAGACATCACCAGAGGAAAGGGAATGGTGGATTCACTCTTCCAAGCCCCCACAGGAGCAGGAACTCATGACCCTGTTCTGAGTTCTTATGAATATTTAAGCGCAGGACTTCGTCAGTACAATTTAGACAACAGCGTGGATGGCTTTTACATTGCTCCTGCCTTCATGGACAAACTAGTTGTTCACATCACTAAGAACTTCATGAAACTTCCCAACATCAAGGTTCCTCTTATTTTGGGTGTTTGGGGAGGCAAAGGTCAAGGAAAATCTTTCCAATGTGAACTTGTCTTTGCCAAGATGGGAATCAACCCCATTATGATGAGTGCCGGAGAATTGGAGAGTGGAAATGCTGGAGAACCTGCAAAATTGATTCGACAGAGGTACCGTGAAGCGGCAGACATTATCAAGAAGGGAAAGATGTGCGTTCTCTTCATCAACGATCTTGATGCCGGAGCTGGTCGTCTCGGTGGAACCACCCAATACACTGTGAACAACCAGATGGTTAACGCTACCCTCATGAACATAGCTGATAACCCTACTAATGTCCAGCTCCCTGGTATGTACAACAAAGAGGACAATCCTCGTGTTCCCATTGTTGTCACGGGTAACGACTTCTCGACATTGTATGCTCCTCTCATCCGTGATGGTCGTATGGAAAAATTCTACTGGGCACCCACACGAGATGATCGAATTGGTGTCTGCTCAGGAATCTTTAGCACTGACAATGTATCAAAAGAAGACATAATCAAAATTGTTGATACATTCCCTGGCCAATCCATTGATTTCTTTGGTGCCCTGAGGGCAAGAATATACGATGATGAAGTGAGGAAGTGGGTTGTTTCCGTTGGAGTTGATAATATCGGGAAGAAGCTCGTCAACTCAAAGGATCCACCGCCGAAATTCGAGCAGCCAAAGATAACATTGAAGAAGCTCCTCGAGTATGGAAACATGCTTGTTGAGGAGCAAGAGAACGTGAAGAGAGTCCAGTTGGCTGACAAGTACTTGAGCGATGCTGCTCTTGGTGATGCCAATGAAGATGCTATAAAAAGTGGAGCATTCTACGGGAAAGCCGCCCAGCAGGTGCATATTCCTGTCCCTGAAGGTTGCACAGACCCAAATGCATCAAACTTTGACCCAACTGCTAGGAGTGATGATGGAAGCTGCAGTTATCAGTTATAAGCCTTCCGCCAATAGCTGTTCGAGTGGAAAAAGATCACCTTCTACTCATGCTATACAAGGAGAATACTGTCACTGACTCACCTGTTTGAATCAAAATAGCCACTGGTTTGCTTTACTGTATGCTTCAGAGTTTGTGGGGGGTTTCAGTTCTCATTTCTTTTTCCCTTTTTTCCCTTGTGTTGCCACGACATTGAGATATCTTTTGTGAGTGTCCATAAAGGACGTAACCTGGCGTCTCATAGATTTTTGTGATATTGAGATATTTTCTTCAGTCTTGATTATACCATACATCCACAAAATGAATGCAGAATCATTAAGGATTGAGAACCTTGAAGACAAAC

Coding sequence (CDS)

ATGGCCACCGCTATCTCCACCGCCGGAGCCGTCAACAGGATTCCGGTGAGTTTAAATGGCGCCATGTCTGGAGCTTCAGTTCAGAGCTCTGCATTCTTCGGCAGCAGCTTGAAGAAAGTGAACTCAAAATCATACCCCAATTCAAAGATCTCCTCAGGAAGCAGCTTTAAGGTAGTTGCTGTGGCAGAGGAAATTGATGAAAAGAAGCAAACTGAAAAGGATAGATGGAAGGGTCTTGCTTTTGATGTCTCTGATGATCAGCAAGACATCACCAGAGGAAAGGGAATGGTGGATTCACTCTTCCAAGCCCCCACAGGAGCAGGAACTCATGACCCTGTTCTGAGTTCTTATGAATATTTAAGCGCAGGACTTCGTCAGTACAATTTAGACAACAGCGTGGATGGCTTTTACATTGCTCCTGCCTTCATGGACAAACTAGTTGTTCACATCACTAAGAACTTCATGAAACTTCCCAACATCAAGGTTCCTCTTATTTTGGGTGTTTGGGGAGGCAAAGGTCAAGGAAAATCTTTCCAATGTGAACTTGTCTTTGCCAAGATGGGAATCAACCCCATTATGATGAGTGCCGGAGAATTGGAGAGTGGAAATGCTGGAGAACCTGCAAAATTGATTCGACAGAGGTACCGTGAAGCGGCAGACATTATCAAGAAGGGAAAGATGTGCGTTCTCTTCATCAACGATCTTGATGCCGGAGCTGGTCGTCTCGGTGGAACCACCCAATACACTGTGAACAACCAGATGGTTAACGCTACCCTCATGAACATAGCTGATAACCCTACTAATGTCCAGCTCCCTGGTATGTACAACAAAGAGGACAATCCTCGTGTTCCCATTGTTGTCACGGGTAACGACTTCTCGACATTGTATGCTCCTCTCATCCGTGATGGTCGTATGGAAAAATTCTACTGGGCACCCACACGAGATGATCGAATTGGTGTCTGCTCAGGAATCTTTAGCACTGACAATGTATCAAAAGAAGACATAATCAAAATTGTTGATACATTCCCTGGCCAATCCATTGATTTCTTTGGTGCCCTGAGGGCAAGAATATACGATGATGAAGTGAGGAAGTGGGTTGTTTCCGTTGGAGTTGATAATATCGGGAAGAAGCTCGTCAACTCAAAGGATCCACCGCCGAAATTCGAGCAGCCAAAGATAACATTGAAGAAGCTCCTCGAGTATGGAAACATGCTTGTTGAGGAGCAAGAGAACGTGAAGAGAGTCCAGTTGGCTGACAAGTACTTGAGCGATGCTGCTCTTGGTGATGCCAATGAAGATGCTATAAAAAGTGGAGCATTCTACGGGAAAGCCGCCCAGCAGGTGCATATTCCTGTCCCTGAAGGTTGCACAGACCCAAATGCATCAAACTTTGACCCAACTGCTAGGAGTGATGATGGAAGCTGCAGTTATCAGTTATAA

Protein sequence

MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVAVAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYLSAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDDEVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADKYLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL
Homology
BLAST of MC04g1908 vs. ExPASy Swiss-Prot
Match: Q7X9A0 (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea tridentata OX=66636 GN=RCA1 PE=1 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 7.5e-224
Identity = 391/481 (81.29%), Postives = 436/481 (90.64%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGAS-VQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVV 60
           MA A ST GAVNR P+SLNG+ + AS V S+AFFGSSLKK  +K +P  K SSG +FK+ 
Sbjct: 1   MAAAYSTVGAVNRAPLSLNGSGARASLVPSTAFFGSSLKKSAAK-FP--KASSG-NFKI- 60

Query: 61  AVAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY 120
            VA+EI E +QT+KD+WKGLA+D+SDDQQDITRGKGMVD+LFQAP  +GTH  V+SSY+Y
Sbjct: 61  -VAQEISEDQQTDKDKWKGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYDY 120

Query: 121 LSAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQ 180
           +S GLRQYNLDN++DGFYIAPAFMDKLVVHITKNF+ LPNIK+PLILG+WGGKGQGKSFQ
Sbjct: 121 ISQGLRQYNLDNNMDGFYIAPAFMDKLVVHITKNFLSLPNIKIPLILGIWGGKGQGKSFQ 180

Query: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240
           CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMC LFINDLDAGA
Sbjct: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDAGA 240

Query: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPL 300
           GR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPI+VTGNDFSTLYAPL
Sbjct: 241 GRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPL 300

Query: 301 IRDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYD 360
           IRDGRMEKFYWAPTR+DRIGVC GIF TDNV+ +DI+K+VDTFPGQSIDFFGALRAR+Y 
Sbjct: 301 IRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADDDIVKLVDTFPGQSIDFFGALRARVYH 360

Query: 361 DEVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLAD 420
           DEVRKWV  VGVD IGKKLVNSK+ PP FEQPK+T+ KLL YG MLV+EQENVKRVQLAD
Sbjct: 361 DEVRKWVSEVGVDTIGKKLVNSKEGPPSFEQPKMTIDKLLGYGGMLVQEQENVKRVQLAD 420

Query: 421 KYLSDAALGDANEDAIKSGAFY-GKAAQQV-HIPVPEGCTDPNASNFDPTARSDDGSCSY 479
           KY+S+AALGDAN DAIK G FY G+AAQQV ++PVPEGCTDP A+N+DPTARSDDGSC Y
Sbjct: 421 KYMSEAALGDANNDAIKRGTFYGGQAAQQVGNVPVPEGCTDPQATNYDPTARSDDGSCVY 475

BLAST of MC04g1908 vs. ExPASy Swiss-Prot
Match: P10896 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RCA PE=1 SV=2)

HSP 1 Score: 751.9 bits (1940), Expect = 4.4e-216
Identity = 374/478 (78.24%), Postives = 415/478 (86.82%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGA-SVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVV 60
           MA A+ST GA+NR P+SLNG+ SGA S  +S F G  +  V+  +  N K  S  SFKV+
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTVSRFAQSNKK--SNGSFKVL 60

Query: 61  AVAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY 120
           AV E+    KQT+ DRW+GLA+D SDDQQDITRGKGMVDS+FQAP G GTH  VLSSYEY
Sbjct: 61  AVKED----KQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEY 120

Query: 121 LSAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQ 180
           +S GLRQYNLDN +DGFYIAPAFMDKLVVHITKNF+ LPNIKVPLILG+WGGKGQGKSFQ
Sbjct: 121 VSQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQ 180

Query: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240
           CELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMC LFINDLDAGA
Sbjct: 181 CELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGA 240

Query: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPL 300
           GR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+N RVPI+ TGNDFSTLYAPL
Sbjct: 241 GRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPL 300

Query: 301 IRDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYD 360
           IRDGRMEKFYWAPTR+DRIGVC GIF TD +  EDI+ +VD FPGQSIDFFGALRAR+YD
Sbjct: 301 IRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYD 360

Query: 361 DEVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLAD 420
           DEVRK+V S+GV+ IGK+LVNS++ PP FEQP++T +KL+EYGNMLV EQENVKRVQLA+
Sbjct: 361 DEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAE 420

Query: 421 KYLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSY 478
            YLS AALGDAN DAI  G FYGK AQQV++PVPEGCTDP A NFDPTARSDDG+C Y
Sbjct: 421 TYLSQAALGDANADAIGRGTFYGKGAQQVNLPVPEGCTDPVAENFDPTARSDDGTCVY 472

BLAST of MC04g1908 vs. ExPASy Swiss-Prot
Match: P93431 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RCA PE=1 SV=2)

HSP 1 Score: 747.3 bits (1928), Expect = 1.1e-214
Identity = 366/448 (81.70%), Postives = 407/448 (90.85%), Query Frame = 0

Query: 32  FFGSSLKK-VNSKSYPNSKISSGSSFKVVAVAEEIDEKKQTEKDRWKGLAFDVSDDQQDI 91
           F G  LKK V S    + K S+ + FKV  +A+E+DE KQT++DRWKGLA+D+SDDQQDI
Sbjct: 19  FLGKKLKKQVTSAVNYHGKSSNINRFKV--MAKELDEGKQTDQDRWKGLAYDISDDQQDI 78

Query: 92  TRGKGMVDSLFQAPTGAGTHDPVLSSYEYLSAGLRQYNLDNSVDGFYIAPAFMDKLVVHI 151
           TRGKG VDSLFQAPTG GTH+ VLSSYEYLS GLR Y+ DN++ GFYIAPAFMDKLVVHI
Sbjct: 79  TRGKGFVDSLFQAPTGDGTHEAVLSSYEYLSQGLRTYDFDNTMGGFYIAPAFMDKLVVHI 138

Query: 152 TKNFMKLPNIKVPLILGVWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKL 211
           +KNFM LPNIKVPLILG+WGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKL
Sbjct: 139 SKNFMTLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKL 198

Query: 212 IRQRYREAADIIKKGKMCVLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQ 271
           IRQRYREAADIIKKGKMC LFINDLDAGAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQ
Sbjct: 199 IRQRYREAADIIKKGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQ 258

Query: 272 LPGMYNKEDNPRVPIVVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRIGVCSGIFSTDNV 331
           LPGMYNKEDNPRVPI+VTGNDFSTLYAPLIRDGRMEKFYWAPTRDDR+GVC GIF TDNV
Sbjct: 259 LPGMYNKEDNPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRVGVCKGIFRTDNV 318

Query: 332 SKEDIIKIVDTFPGQSIDFFGALRARIYDDEVRKWVVSVGVDNIGKKLVNSKDPPPKFEQ 391
             EDI+KIVD+FPGQSIDFFGALRAR+YDDEVRKWV   GV+NIGK+LVNS++ PP+FEQ
Sbjct: 319 PDEDIVKIVDSFPGQSIDFFGALRARVYDDEVRKWVSDTGVENIGKRLVNSREGPPEFEQ 378

Query: 392 PKITLKKLLEYGNMLVEEQENVKRVQLADKYLSDAALGDANEDAIKSGAFYGKAAQQV-H 451
           PK+T++KL+EYG MLV+EQENVKRVQLA++YLS+AALGDAN DA+K+G+FYG+ AQQ  +
Sbjct: 379 PKMTIEKLMEYGYMLVKEQENVKRVQLAEQYLSEAALGDANSDAMKTGSFYGQGAQQAGN 438

Query: 452 IPVPEGCTDPNASNFDPTARSDDGSCSY 478
           +PVPEGCTDP A NFDPTARSDDGSC Y
Sbjct: 439 LPVPEGCTDPVAKNFDPTARSDDGSCLY 464

BLAST of MC04g1908 vs. ExPASy Swiss-Prot
Match: Q40281 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus domestica OX=3750 GN=RCA PE=2 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 9.2e-214
Identity = 364/442 (82.35%), Postives = 404/442 (91.40%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60
           MATA+ST G+VNR P +LNG+ S ASV SS F GSSLKKVNS+ + NSK+SSGS    + 
Sbjct: 1   MATAVSTIGSVNRAPPNLNGSSSSASVPSSTFLGSSLKKVNSR-FTNSKVSSGS----LR 60

Query: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120
           +   +DE KQT+KDRWKGLAFD SDDQQDITRGKG VDSLFQAP G+GTH  ++SSYEY+
Sbjct: 61  IVASVDEDKQTDKDRWKGLAFDTSDDQQDITRGKGKVDSLFQAPQGSGTHFAIMSSYEYI 120

Query: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           S GLRQYN DN++DG+YIAPAFMDKLVVHITKNFM LPN+KVPLILG+WGGKGQGKSFQC
Sbjct: 121 STGLRQYNFDNNMDGYYIAPAFMDKLVVHITKNFMTLPNMKVPLILGIWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELVFAKM I+PIMMSAGELESGNAGEPAKLIRQRYREAADII+KGKMC LFINDLDAGAG
Sbjct: 181 ELVFAKMRISPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCALFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPI+VTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD 360
           RDGRMEKFYWAPTR+DRIGVC GIF +DNV+KEDI+K+VDTFPGQSIDFFGALRAR+YDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCIGIFRSDNVAKEDIVKLVDTFPGQSIDFFGALRARVYDD 360

Query: 361 EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK 420
           EVRKW+  VGVD+IGKKLVNSK+ PP FEQPK+T++KLLEYGNMLV+EQENVKRVQLADK
Sbjct: 361 EVRKWITGVGVDSIGKKLVNSKEGPPTFEQPKMTIEKLLEYGNMLVQEQENVKRVQLADK 420

Query: 421 YLSDAALGDANEDAIKSGAFYG 443
           YLS+AALGDAN DA+ +G FYG
Sbjct: 421 YLSEAALGDANSDAMNTGTFYG 437

BLAST of MC04g1908 vs. ExPASy Swiss-Prot
Match: P10871 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=2)

HSP 1 Score: 738.4 bits (1905), Expect = 5.1e-212
Identity = 361/479 (75.37%), Postives = 410/479 (85.59%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60
           MATA+ST GA  R P++LNG+ +GASV +S F GSSLKK  +  +P+S  ++  + K   
Sbjct: 1   MATAVSTVGAATRAPLNLNGSSAGASVPTSGFLGSSLKKHTNVRFPSSSRTTSMTVKAA- 60

Query: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120
                 E ++   D+W  LA D SDDQ DI RGKGMVDSLFQAP  AGTH P+ SS+EY 
Sbjct: 61  ------ENEEKNTDKWAHLAKDFSDDQLDIRRGKGMVDSLFQAPADAGTHVPIQSSFEYE 120

Query: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           S GLR+Y++DN +  FYIAPAFMDKLVVHITKNF+ LPNIK+PLILGVWGGKGQGKSFQC
Sbjct: 121 SQGLRKYDIDNMLGDFYIAPAFMDKLVVHITKNFLNLPNIKIPLILGVWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELVFAK+GINPIMMSAGELESGNAGEPAKLIRQRYREAAD+I KGKMC LFINDL+ GAG
Sbjct: 181 ELVFAKLGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIAKGKMCALFINDLEPGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI 300
           R+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNK+DN RVPI+VTGNDFSTLYAPLI
Sbjct: 241 RMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQDNARVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD 360
           RDGRMEKFYWAPTR+DRIGVC+GIF TD V  E ++K+VD FPGQSIDFFGALRAR+Y D
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCTGIFKTDKVPAEHVVKLVDAFPGQSIDFFGALRARVYHD 360

Query: 361 EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK 420
           EVRKWV SVGVDN+GKKLVNSKD PP FEQP++TL+KL+EYGNMLV+EQENVKRVQLAD+
Sbjct: 361 EVRKWVNSVGVDNVGKKLVNSKDGPPVFEQPEMTLQKLMEYGNMLVQEQENVKRVQLADQ 420

Query: 421 YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 480
           Y+S AALGDAN+DAI  G F+GKAAQQV +PV +GCTDP A N+DPTARSDDGSC+Y L
Sbjct: 421 YMSSAALGDANKDAIDRGTFFGKAAQQVSLPVAQGCTDPEAKNYDPTARSDDGSCTYNL 472

BLAST of MC04g1908 vs. NCBI nr
Match: XP_022134517.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 944 bits (2441), Expect = 0.0
Identity = 479/479 (100.00%), Postives = 479/479 (100.00%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60
           MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA
Sbjct: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60

Query: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120
           VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL
Sbjct: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120

Query: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC
Sbjct: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG
Sbjct: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD 360
           RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD
Sbjct: 301 RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD 360

Query: 361 EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK 420
           EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK
Sbjct: 361 EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK 420

Query: 421 YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479
           YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL
Sbjct: 421 YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479

BLAST of MC04g1908 vs. NCBI nr
Match: XP_022955346.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita moschata] >XP_023552446.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6602653.1 Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033339.1 Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 435/479 (90.81%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60
           MATA+STAGAVNRIPVSLNGA+SGASVQSSAF GSSLKKVNS+ YPNSK+S GSS KVVA
Sbjct: 1   MATALSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKVNSR-YPNSKVSWGSSLKVVA 60

Query: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120
           VAEEIDEKKQT+KDRWKGLA+D+SDDQQDITRGKG+VDSLFQAPTGAGTHDPVLSSYEYL
Sbjct: 61  VAEEIDEKKQTDKDRWKGLAYDISDDQQDITRGKGLVDSLFQAPTGAGTHDPVLSSYEYL 120

Query: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           SAGLRQYNLDN+VDGFYIAP+FMDKLVVHI+KNFMKLPNIKVPLILGVWGGKGQGKSFQC
Sbjct: 121 SAGLRQYNLDNNVDGFYIAPSFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG
Sbjct: 181 ELVLAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPI+VTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD 360
           RDGRMEKFYWAPTR+DRIGVC+GIF +DNV KEDI+K+VDTFPGQSIDFFGALRAR+YDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVPKEDIVKLVDTFPGQSIDFFGALRARVYDD 360

Query: 361 EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK 420
           EVRKW  SVGV+NI K+LVNSK+ PP FEQPK+TL+KLLEYGNMLV+EQENVKRVQLADK
Sbjct: 361 EVRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLADK 420

Query: 421 YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479
           YLS+AALGDANED+IKSGAFYGKAAQQV+ PVPEGCTDPNA NFDPTARSD+GSC+Y+L
Sbjct: 421 YLSEAALGDANEDSIKSGAFYGKAAQQVNFPVPEGCTDPNAENFDPTARSDNGSCNYEL 478

BLAST of MC04g1908 vs. NCBI nr
Match: XP_022991087.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 435/479 (90.81%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60
           MATA+STAGAVNRIPVSLNGA+SGASVQSSAF GSSLKKVNS+ YPNSK+S GSS KVVA
Sbjct: 1   MATALSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKVNSR-YPNSKVSWGSSLKVVA 60

Query: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120
           VAEEIDEKKQT+KDRWKGLA+D+SDDQQDITRGKG+VDSLFQAPTGAGTHDPVL+SYEYL
Sbjct: 61  VAEEIDEKKQTDKDRWKGLAYDISDDQQDITRGKGLVDSLFQAPTGAGTHDPVLNSYEYL 120

Query: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           SAGLRQYNLDN+VDGFYIAP+FMDKLVVHI+KNFMKLPNIKVPLILGVWGGKGQGKSFQC
Sbjct: 121 SAGLRQYNLDNNVDGFYIAPSFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG
Sbjct: 181 ELVLAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPI+VTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD 360
           RDGRMEKFYWAPTR+DRIGVC+GIF +DNV KEDI+K+VDTFPGQSIDFFGALRAR+YDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVPKEDIVKLVDTFPGQSIDFFGALRARVYDD 360

Query: 361 EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK 420
           EVRKW  SVGV+NI K+LVNSK+ PP FEQPK+TL+KLLEYGNMLV+EQENVKRVQLADK
Sbjct: 361 EVRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLADK 420

Query: 421 YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479
           YLS+AALGDANEDAIKSGAFYGKAAQQV+ PVPEGCTDPNA NFDPTARSD+GSC+Y+L
Sbjct: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVNFPVPEGCTDPNAENFDPTARSDNGSCNYEL 478

BLAST of MC04g1908 vs. NCBI nr
Match: XP_038886619.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Benincasa hispida])

HSP 1 Score: 869 bits (2245), Expect = 0.0
Identity = 432/480 (90.00%), Postives = 462/480 (96.25%), Query Frame = 0

Query: 1   MATA-ISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVV 60
           MATA +STAGAVNRIP+SLNGA++G SVQSSAF G+SLKK N + YPNSK+SSGSS KVV
Sbjct: 1   MATAAVSTAGAVNRIPLSLNGAIAGPSVQSSAFLGNSLKKANLR-YPNSKVSSGSSLKVV 60

Query: 61  AVAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY 120
           AVAEEIDEKKQT KD+WKGLAFD+SDDQQDITRGKGMVD+LFQAP+GAGTHDPVLSSYEY
Sbjct: 61  AVAEEIDEKKQTNKDKWKGLAFDISDDQQDITRGKGMVDTLFQAPSGAGTHDPVLSSYEY 120

Query: 121 LSAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQ 180
           LSAGLRQYNLDN+VDGFYIAPAFMDKLVVHI+KNFMKLPNIKVPLILG+WGGKGQGKSFQ
Sbjct: 121 LSAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGIWGGKGQGKSFQ 180

Query: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240
           CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA
Sbjct: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240

Query: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPL 300
           GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPIVVTGNDFSTLYAPL
Sbjct: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPL 300

Query: 301 IRDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYD 360
           IRDGRMEKFYWAPTR+DRIGVCSGIF +DNV KED++K+VDTFPGQSIDFFGALRAR+YD
Sbjct: 301 IRDGRMEKFYWAPTREDRIGVCSGIFRSDNVPKEDVVKLVDTFPGQSIDFFGALRARVYD 360

Query: 361 DEVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLAD 420
           DEVR W +SVGV+NI KKLVNSK+ PP FEQPK+TL+KLLEYGNMLV+EQENVKRVQLAD
Sbjct: 361 DEVRNWAISVGVENIAKKLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLAD 420

Query: 421 KYLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479
           KYLS+AALGDANEDAIKSG FYGKAAQQVH+PVPEGCTDPNA NFDPTARSDDGSC+Y L
Sbjct: 421 KYLSEAALGDANEDAIKSGTFYGKAAQQVHLPVPEGCTDPNADNFDPTARSDDGSCNYVL 479

BLAST of MC04g1908 vs. NCBI nr
Match: XP_023550769.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 867 bits (2239), Expect = 1.60e-315
Identity = 431/480 (89.79%), Postives = 462/480 (96.25%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSF-KVV 60
           MA A+STAGAVN + +SLNGA++GASVQ+SAF GSSLKK N + YPNSK+SSG S  KVV
Sbjct: 1   MAAAVSTAGAVNGVSLSLNGAIAGASVQNSAFLGSSLKKANFR-YPNSKVSSGRSLMKVV 60

Query: 61  AVAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY 120
           AVAE+IDEKKQT+KDRWKGLA+D+SDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY
Sbjct: 61  AVAEDIDEKKQTDKDRWKGLAYDISDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY 120

Query: 121 LSAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQ 180
           LSAGLRQYNLDN+VDGFYIAPAFMDKLVVHI+KNFMKLPNIKVPLILG+WGGKGQGKSFQ
Sbjct: 121 LSAGLRQYNLDNTVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGIWGGKGQGKSFQ 180

Query: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240
           CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA
Sbjct: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240

Query: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPL 300
           GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPI+VTGNDFSTLYAPL
Sbjct: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPL 300

Query: 301 IRDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYD 360
           IRDGRMEKFYWAPTR+DRIGVCSGIF TDNV KEDIIK+VDTFPGQSIDFFGALRAR+YD
Sbjct: 301 IRDGRMEKFYWAPTREDRIGVCSGIFRTDNVPKEDIIKLVDTFPGQSIDFFGALRARVYD 360

Query: 361 DEVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLAD 420
           DEVRKW  SVGV+NI K+LVNSK+ PP FEQPK+TL+KLLEYGNMLV+EQENVKRVQLAD
Sbjct: 361 DEVRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLQKLLEYGNMLVQEQENVKRVQLAD 420

Query: 421 KYLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479
           KYLS+AALGDANEDAIKSGAFYGKAAQQVH+PVPEGCTDPNA N+DPTARSDDGSC+Y+L
Sbjct: 421 KYLSEAALGDANEDAIKSGAFYGKAAQQVHLPVPEGCTDPNAENYDPTARSDDGSCNYEL 479

BLAST of MC04g1908 vs. ExPASy TrEMBL
Match: A0A6J1BZT9 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111006741 PE=4 SV=1)

HSP 1 Score: 944 bits (2441), Expect = 0.0
Identity = 479/479 (100.00%), Postives = 479/479 (100.00%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60
           MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA
Sbjct: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60

Query: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120
           VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL
Sbjct: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120

Query: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC
Sbjct: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG
Sbjct: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD 360
           RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD
Sbjct: 301 RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD 360

Query: 361 EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK 420
           EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK
Sbjct: 361 EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK 420

Query: 421 YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479
           YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL
Sbjct: 421 YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479

BLAST of MC04g1908 vs. ExPASy TrEMBL
Match: A0A6J1JKS4 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487787 PE=4 SV=1)

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 435/479 (90.81%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60
           MATA+STAGAVNRIPVSLNGA+SGASVQSSAF GSSLKKVNS+ YPNSK+S GSS KVVA
Sbjct: 1   MATALSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKVNSR-YPNSKVSWGSSLKVVA 60

Query: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120
           VAEEIDEKKQT+KDRWKGLA+D+SDDQQDITRGKG+VDSLFQAPTGAGTHDPVL+SYEYL
Sbjct: 61  VAEEIDEKKQTDKDRWKGLAYDISDDQQDITRGKGLVDSLFQAPTGAGTHDPVLNSYEYL 120

Query: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           SAGLRQYNLDN+VDGFYIAP+FMDKLVVHI+KNFMKLPNIKVPLILGVWGGKGQGKSFQC
Sbjct: 121 SAGLRQYNLDNNVDGFYIAPSFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG
Sbjct: 181 ELVLAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPI+VTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD 360
           RDGRMEKFYWAPTR+DRIGVC+GIF +DNV KEDI+K+VDTFPGQSIDFFGALRAR+YDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVPKEDIVKLVDTFPGQSIDFFGALRARVYDD 360

Query: 361 EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK 420
           EVRKW  SVGV+NI K+LVNSK+ PP FEQPK+TL+KLLEYGNMLV+EQENVKRVQLADK
Sbjct: 361 EVRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLADK 420

Query: 421 YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479
           YLS+AALGDANEDAIKSGAFYGKAAQQV+ PVPEGCTDPNA NFDPTARSD+GSC+Y+L
Sbjct: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVNFPVPEGCTDPNAENFDPTARSDNGSCNYEL 478

BLAST of MC04g1908 vs. ExPASy TrEMBL
Match: A0A6J1GTI7 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111457396 PE=4 SV=1)

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 435/479 (90.81%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60
           MATA+STAGAVNRIPVSLNGA+SGASVQSSAF GSSLKKVNS+ YPNSK+S GSS KVVA
Sbjct: 1   MATALSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKVNSR-YPNSKVSWGSSLKVVA 60

Query: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120
           VAEEIDEKKQT+KDRWKGLA+D+SDDQQDITRGKG+VDSLFQAPTGAGTHDPVLSSYEYL
Sbjct: 61  VAEEIDEKKQTDKDRWKGLAYDISDDQQDITRGKGLVDSLFQAPTGAGTHDPVLSSYEYL 120

Query: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           SAGLRQYNLDN+VDGFYIAP+FMDKLVVHI+KNFMKLPNIKVPLILGVWGGKGQGKSFQC
Sbjct: 121 SAGLRQYNLDNNVDGFYIAPSFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG
Sbjct: 181 ELVLAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPI+VTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDD 360
           RDGRMEKFYWAPTR+DRIGVC+GIF +DNV KEDI+K+VDTFPGQSIDFFGALRAR+YDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVPKEDIVKLVDTFPGQSIDFFGALRARVYDD 360

Query: 361 EVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADK 420
           EVRKW  SVGV+NI K+LVNSK+ PP FEQPK+TL+KLLEYGNMLV+EQENVKRVQLADK
Sbjct: 361 EVRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLADK 420

Query: 421 YLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479
           YLS+AALGDANED+IKSGAFYGKAAQQV+ PVPEGCTDPNA NFDPTARSD+GSC+Y+L
Sbjct: 421 YLSEAALGDANEDSIKSGAFYGKAAQQVNFPVPEGCTDPNAENFDPTARSDNGSCNYEL 478

BLAST of MC04g1908 vs. ExPASy TrEMBL
Match: A0A6J1FKE0 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111444819 PE=4 SV=1)

HSP 1 Score: 865 bits (2235), Expect = 1.55e-315
Identity = 430/480 (89.58%), Postives = 461/480 (96.04%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSF-KVV 60
           MA A+STAGA N + +SLNGA++GASVQ+SAF GSSLKK N + YPNSK+SSG S  KVV
Sbjct: 1   MAAAVSTAGATNGVSLSLNGAIAGASVQNSAFLGSSLKKANFR-YPNSKVSSGRSLMKVV 60

Query: 61  AVAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY 120
           AVAE+IDEKKQT+KDRWKGLA+D+SDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY
Sbjct: 61  AVAEDIDEKKQTDKDRWKGLAYDISDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY 120

Query: 121 LSAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQ 180
           LSAGLRQYNLDN+VDGFYIAPAFMDKLVVHI+KNFMKLPNIKVPLILG+WGGKGQGKSFQ
Sbjct: 121 LSAGLRQYNLDNTVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGIWGGKGQGKSFQ 180

Query: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240
           CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA
Sbjct: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240

Query: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPL 300
           GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPI+VTGNDFSTLYAPL
Sbjct: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPL 300

Query: 301 IRDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYD 360
           IRDGRMEKFYWAPTR+DRIGVCSGIF TDNV KEDIIK+VDTFPGQSIDFFGALRAR+YD
Sbjct: 301 IRDGRMEKFYWAPTREDRIGVCSGIFRTDNVPKEDIIKLVDTFPGQSIDFFGALRARVYD 360

Query: 361 DEVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLAD 420
           DEVRKW  SVGV+NI K+LVNSK+ PP FEQPK+TL+KLLEYGNMLV+EQENVKRVQLAD
Sbjct: 361 DEVRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLQKLLEYGNMLVQEQENVKRVQLAD 420

Query: 421 KYLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479
           KYLS+AALGDANEDAIKSGAFYGKAAQQVH+PVPEGCTDPNA N+DPTARSDDGSC+Y+L
Sbjct: 421 KYLSEAALGDANEDAIKSGAFYGKAAQQVHLPVPEGCTDPNAENYDPTARSDDGSCNYEL 479

BLAST of MC04g1908 vs. ExPASy TrEMBL
Match: A0A6J1JQC9 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488905 PE=4 SV=1)

HSP 1 Score: 859 bits (2219), Expect = 4.04e-313
Identity = 426/474 (89.87%), Postives = 457/474 (96.41%), Query Frame = 0

Query: 7   TAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSF-KVVAVAEEI 66
           TAGAVN + +SLNGA++GASVQ+SAF GSSLKK N + YPNS++SSG S  KVVAVAE+I
Sbjct: 4   TAGAVNGVSLSLNGAIAGASVQNSAFLGSSLKKANFR-YPNSRVSSGRSLMKVVAVAEDI 63

Query: 67  DEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYLSAGLR 126
           DEKKQT+KDRWKGLA+D+SDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYLSAGLR
Sbjct: 64  DEKKQTDKDRWKGLAYDISDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYLSAGLR 123

Query: 127 QYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQCELVFA 186
           QYNLDN+VDGFYIAPAFMDKLVVHI+KNFMKLPNIKVPLILG+WGGKGQGKSFQCELVFA
Sbjct: 124 QYNLDNTVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGIWGGKGQGKSFQCELVFA 183

Query: 187 KMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGRLGGT 246
           KMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGRLGGT
Sbjct: 184 KMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGRLGGT 243

Query: 247 TQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPLIRDGRM 306
           TQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPI+VTGNDFSTLYAPLIRDGRM
Sbjct: 244 TQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRM 303

Query: 307 EKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYDDEVRKW 366
           EKFYWAPTR+DRIGVCSGIF TDNV KEDIIK+VDTFPGQSIDFFGALRAR+YDDEVRKW
Sbjct: 304 EKFYWAPTREDRIGVCSGIFRTDNVPKEDIIKLVDTFPGQSIDFFGALRARVYDDEVRKW 363

Query: 367 VVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLADKYLSDA 426
             SVGV+NI K+LVNSK+ PP FEQPK+TL+KLLEYGNMLV+EQENVKRVQLADKYLS+A
Sbjct: 364 AASVGVENISKRLVNSKEGPPTFEQPKMTLQKLLEYGNMLVQEQENVKRVQLADKYLSEA 423

Query: 427 ALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSYQL 479
           ALGDANEDAIKSGAFYGKAAQQVH+PVPEGCTDPNA N+DPTARSDDGSC+Y+L
Sbjct: 424 ALGDANEDAIKSGAFYGKAAQQVHLPVPEGCTDPNAENYDPTARSDDGSCNYEL 476

BLAST of MC04g1908 vs. TAIR 10
Match: AT2G39730.1 (rubisco activase )

HSP 1 Score: 751.9 bits (1940), Expect = 3.1e-217
Identity = 374/478 (78.24%), Postives = 415/478 (86.82%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGA-SVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVV 60
           MA A+ST GA+NR P+SLNG+ SGA S  +S F G  +  V+  +  N K  S  SFKV+
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTVSRFAQSNKK--SNGSFKVL 60

Query: 61  AVAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY 120
           AV E+    KQT+ DRW+GLA+D SDDQQDITRGKGMVDS+FQAP G GTH  VLSSYEY
Sbjct: 61  AVKED----KQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEY 120

Query: 121 LSAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQ 180
           +S GLRQYNLDN +DGFYIAPAFMDKLVVHITKNF+ LPNIKVPLILG+WGGKGQGKSFQ
Sbjct: 121 VSQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQ 180

Query: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240
           CELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMC LFINDLDAGA
Sbjct: 181 CELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGA 240

Query: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPL 300
           GR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+N RVPI+ TGNDFSTLYAPL
Sbjct: 241 GRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPL 300

Query: 301 IRDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYD 360
           IRDGRMEKFYWAPTR+DRIGVC GIF TD +  EDI+ +VD FPGQSIDFFGALRAR+YD
Sbjct: 301 IRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYD 360

Query: 361 DEVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLAD 420
           DEVRK+V S+GV+ IGK+LVNS++ PP FEQP++T +KL+EYGNMLV EQENVKRVQLA+
Sbjct: 361 DEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAE 420

Query: 421 KYLSDAALGDANEDAIKSGAFYGKAAQQVHIPVPEGCTDPNASNFDPTARSDDGSCSY 478
            YLS AALGDAN DAI  G FYGK AQQV++PVPEGCTDP A NFDPTARSDDG+C Y
Sbjct: 421 TYLSQAALGDANADAIGRGTFYGKGAQQVNLPVPEGCTDPVAENFDPTARSDDGTCVY 472

BLAST of MC04g1908 vs. TAIR 10
Match: AT2G39730.2 (rubisco activase )

HSP 1 Score: 694.9 bits (1792), Expect = 4.6e-200
Identity = 347/448 (77.46%), Postives = 387/448 (86.38%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGA-SVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVV 60
           MA A+ST GA+NR P+SLNG+ SGA S  +S F G  +  V+  +  N K  S  SFKV+
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTVSRFAQSNKK--SNGSFKVL 60

Query: 61  AVAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY 120
           AV E+    KQT+ DRW+GLA+D SDDQQDITRGKGMVDS+FQAP G GTH  VLSSYEY
Sbjct: 61  AVKED----KQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEY 120

Query: 121 LSAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQ 180
           +S GLRQYNLDN +DGFYIAPAFMDKLVVHITKNF+ LPNIKVPLILG+WGGKGQGKSFQ
Sbjct: 121 VSQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQ 180

Query: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240
           CELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMC LFINDLDAGA
Sbjct: 181 CELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGA 240

Query: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPL 300
           GR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+N RVPI+ TGNDFSTLYAPL
Sbjct: 241 GRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPL 300

Query: 301 IRDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYD 360
           IRDGRMEKFYWAPTR+DRIGVC GIF TD +  EDI+ +VD FPGQSIDFFGALRAR+YD
Sbjct: 301 IRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYD 360

Query: 361 DEVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLAD 420
           DEVRK+V S+GV+ IGK+LVNS++ PP FEQP++T +KL+EYGNMLV EQENVKRVQLA+
Sbjct: 361 DEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAE 420

Query: 421 KYLSDAALGDANEDAIKSGAFYGKAAQQ 448
            YLS AALGDAN DAI  G FYGK  ++
Sbjct: 421 TYLSQAALGDANADAIGRGTFYGKTEEK 442

BLAST of MC04g1908 vs. TAIR 10
Match: AT2G39730.3 (rubisco activase )

HSP 1 Score: 694.1 bits (1790), Expect = 7.8e-200
Identity = 347/444 (78.15%), Postives = 385/444 (86.71%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGA-SVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVV 60
           MA A+ST GA+NR P+SLNG+ SGA S  +S F G  +  V+  +  N K  S  SFKV+
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTVSRFAQSNKK--SNGSFKVL 60

Query: 61  AVAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEY 120
           AV E+    KQT+ DRW+GLA+D SDDQQDITRGKGMVDS+FQAP G GTH  VLSSYEY
Sbjct: 61  AVKED----KQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEY 120

Query: 121 LSAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLPNIKVPLILGVWGGKGQGKSFQ 180
           +S GLRQYNLDN +DGFYIAPAFMDKLVVHITKNF+ LPNIKVPLILG+WGGKGQGKSFQ
Sbjct: 121 VSQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQ 180

Query: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGA 240
           CELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMC LFINDLDAGA
Sbjct: 181 CELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGA 240

Query: 241 GRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIVVTGNDFSTLYAPL 300
           GR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+N RVPI+ TGNDFSTLYAPL
Sbjct: 241 GRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPL 300

Query: 301 IRDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARIYD 360
           IRDGRMEKFYWAPTR+DRIGVC GIF TD +  EDI+ +VD FPGQSIDFFGALRAR+YD
Sbjct: 301 IRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYD 360

Query: 361 DEVRKWVVSVGVDNIGKKLVNSKDPPPKFEQPKITLKKLLEYGNMLVEEQENVKRVQLAD 420
           DEVRK+V S+GV+ IGK+LVNS++ PP FEQP++T +KL+EYGNMLV EQENVKRVQLA+
Sbjct: 361 DEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAE 420

Query: 421 KYLSDAALGDANEDAIKSGAFYGK 444
            YLS AALGDAN DAI  G FYGK
Sbjct: 421 TYLSQAALGDANADAIGRGTFYGK 438

BLAST of MC04g1908 vs. TAIR 10
Match: AT1G73110.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 3.6e-88
Identity = 183/430 (42.56%), Postives = 266/430 (61.86%), Query Frame = 0

Query: 1   MATAISTAGAVNRIPVSLNGAMSGASVQSSAFFGSSLKKVNSKSYPNSKISSGSSFKVVA 60
           + ++ S++ + N   ++     S    + S+  G  +   +  + P  K+S  SS++V  
Sbjct: 15  LQSSSSSSSSFNATLINTRKLSSIVCSKPSSNDGGKVANDDGGAKPRKKLSEQSSWEV-- 74

Query: 61  VAEEIDEKKQTEKDRWKGLAFDVSDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120
                  K    KD    L  +  +    +    GM+D +F         D V   ++Y 
Sbjct: 75  -------KDSEGKDYLYRLGAESDNVNIAVGARSGMIDDVFIGDFLGKDSDIV---FDYR 134

Query: 121 SAGLRQYNLDNSVDGFYIAPAFMDKLVVHITKNFMKLP-NIKVPLILGVWGGKGQGKSFQ 180
               R +  ++    +YIAP+F+DK+ VHI KN++    NIK+PLILG+WGGKGQGK+FQ
Sbjct: 135 QKATRSF--EHLQGDYYIAPSFLDKVAVHIVKNYLAPSLNIKIPLILGIWGGKGQGKTFQ 194

Query: 181 CELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIK-KGKMCVLFINDLDAG 240
            EL+F  MG+ P++MSAGELES  AGEP +LIR RYR A+ +I+ +GKM VL IND+DAG
Sbjct: 195 TELIFKTMGVEPVIMSAGELESDRAGEPGRLIRDRYRTASQVIQNQGKMSVLMINDIDAG 254

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDN-PRVPIVVTGNDFSTLYA 300
            GR  G TQ TVNNQ+V  TLMN+ADNPT V +   + + D   RVP++VTGNDFSTLYA
Sbjct: 255 LGRF-GETQMTVNNQIVVGTLMNLADNPTRVSVGQEWREADMVNRVPLIVTGNDFSTLYA 314

Query: 301 PLIRDGRMEKFYWAPTRDDRIGVCSGIFSTDNVSKEDIIKIVDTFPGQSIDFFGALRARI 360
           PLIR+GRMEKFYW PTR+D + + S ++  D +S++D+I IVD FP Q++DF+GALR+R 
Sbjct: 315 PLIREGRMEKFYWQPTREDIVNIVSRMYEKDGISRKDVISIVDKFPNQALDFYGALRSRT 374

Query: 361 YDDEVRKWVVSV-GVDNIGKKLVNSK--DPPPKFEQPKITLKKLLEYGNMLVEEQENVKR 420
           YD  + KWV    G++ +GK L+  K     P+F  P+ T++ LLE G  L+ EQ+ +  
Sbjct: 375 YDRSILKWVDEAGGMETLGKVLLRRKKTQEVPQFTAPEQTVEALLESGYSLINEQKLIME 429

Query: 421 VQLADKYLSD 425
            +L+ +Y+ +
Sbjct: 435 TKLSKEYMKN 429

BLAST of MC04g1908 vs. TAIR 10
Match: AT2G03670.1 (cell division cycle 48B )

HSP 1 Score: 48.9 bits (115), Expect = 1.3e-05
Identity = 40/163 (24.54%), Postives = 72/163 (44.17%), Query Frame = 0

Query: 160 IKVPLILGVWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAA 219
           +K P  L ++G  G GK+     V  +   + I++S   +   +AGE  K++R+ + EA+
Sbjct: 53  LKWPRGLLLYGPPGTGKTSLVRAVVQECDAHLIVLSPHSVHRAHAGESEKVLREAFAEAS 112

Query: 220 DIIKKGKMCVLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKED 279
                 K  V+FI+++D    R     +  V       TLM+ ++ P++           
Sbjct: 113 SHAVSDKPSVIFIDEIDVLCPRRDARREQDVRIASQLFTLMD-SNKPSS----------S 172

Query: 280 NPRVPIVVTGNDFSTLYAPLIRDGRMEKF--YWAPTRDDRIGV 321
            PRV +V + N    +   L R GR +       P  +DR+ +
Sbjct: 173 APRVVVVASTNRVDAIDPALRRAGRFDALVEVSTPNEEDRLKI 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7X9A07.5e-22481.29Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea ... [more]
P108964.4e-21678.24Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Arabidops... [more]
P934311.1e-21481.70Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Oryza sat... [more]
Q402819.2e-21482.35Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus dom... [more]
P108715.1e-21275.37Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Spinacia ... [more]
Match NameE-valueIdentityDescription
XP_022134517.10.0100.00ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Momord... [more]
XP_022955346.10.090.81ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita m... [more]
XP_022991087.10.090.81ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita m... [more]
XP_038886619.10.090.00ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Benincasa h... [more]
XP_023550769.11.60e-31589.79ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurb... [more]
Match NameE-valueIdentityDescription
A0A6J1BZT90.0100.00ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Momo... [more]
A0A6J1JKS40.090.81ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita... [more]
A0A6J1GTI70.090.81ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita... [more]
A0A6J1FKE01.55e-31589.58ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Cucu... [more]
A0A6J1JQC94.04e-31389.87ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Cucu... [more]
Match NameE-valueIdentityDescription
AT2G39730.13.1e-21778.24rubisco activase [more]
AT2G39730.24.6e-20077.46rubisco activase [more]
AT2G39730.37.8e-20078.15rubisco activase [more]
AT1G73110.13.6e-8842.56P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G03670.11.3e-0524.54cell division cycle 48B [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 168..309
e-value: 1.1E-14
score: 55.0
NoneNo IPR availableGENE3D1.10.8.1070coord: 313..425
e-value: 1.8E-49
score: 168.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 453..479
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 460..479
NoneNo IPR availablePANTHERPTHR32429:SF25RIBULOSE BISPHOSPHATE CARBOXYLASE/OXYGENASE ACTIVASE, CHLOROPLASTIC-LIKE ISOFORM X1coord: 1..478
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 128..312
e-value: 1.2E-43
score: 150.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 140..337
IPR044960Ribulose bisphosphate carboxylase/oxygenase activase-likePANTHERPTHR32429FAMILY NOT NAMEDcoord: 1..478

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g1908.1MC04g1908.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0046863 ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity