MC04g1838 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g1838
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	alpha-soluble NSF attachment protein 2
Location	MC04: 25592692 .. 25597263 (+)
RNA-Seq Expression	MC04g1838
Synteny	MC04g1838

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGATCAGCTTGCCAAGGGAGAGGAATTCGAGAAGAAAGCGGAGAAGAAGCTCAATGGATGGGGATTGTTCGGTTCCAAGTACGAAGATGCAGCTGACCTCTTCGATAAGGCTGCTAACTGTTACAAGCTTTCTAAATCATGTAATTCACCATAATCTTTCTCTGCAACTTCTTCTTTTTGGTCCCTTAGATCTTTGCGCCATCGATTCATTCAATTTTTCTCGTCATTATGCATTCACTTGGACTGAACTTTATTGTCTGTTACTTGAATTAAAAGGAATTTATTGTCGATCAAACAGAGAGCTACCTGCTGGTTCTTCTGGTTGGAATTTGACCTACATAGTTGTTTTTACTGAAATTAGAATTTATTTTGACGATCCTGCTAGCAAGGTATGTACTTGATTTGAGCTAGGAGGGTTAGGACTGAATGAGTCAATAATCTGATGGATTGTAAAGGTATTTTTATGGCACAATAAATGCATTTGGTTTATGTTCAGTTTATAGATGCAACTGTCTGTACTCTATCCGGATATACGATGATACAATCTAAGAGTTGTCTACAAATGATAGCTAGACACAGTATAAAAAGGTTTGGATGCGTTAACAATCCAAATTACAATTTTGGATCATATTGTTTGGGAACCAGACACAATAACTGAAAACTATATTTAGAAATCCATAAATGTGTTAGCCGTGCAATGCAAAATGTGAAGGACATAGTGAAAATTAAATGGTTTGGTTGCAGACATATTGGCCAGAGGGACGAAGAAATGTAAACCAAAAGGACATGTTTGAACACTGTTTTTAGATTTTTCTTAATCTTCAAAAGGACATGTTTTCCCCTAAATAAAAAGGACATATTTATTCCCCTAATTGTGGCATCGGTCTCTTTTCTCAGTAGAAAGAATATAAAAACAGTCACTTTCCCCAAAATTATGTAGTCTAATAATTTTGTTATCATTCACAGTTACCTCATCTCTTCTCACTATTTCACCTGTTATGTGGCTTTAATCTCTATGCATCCTGTGTTTTAGGGGATAAAGCTGGGTCAACATATACAAAGTTGGCAAGTTGCCATCTGAAGGTAAGTGCTTCAAATATTTTATAGTGTATTACCTCATAAAATGACCTTTCCGACATCATTTTAGAAATCATTTTTTTGTAGGTGGAAAGTAAGCATGAAGCTGCTCAAGCTTATGTTGATGCTGCCAATTCCTATAAGAAAACATCTATAAAGGGTTGAGTCCCATACTGCTCTTTATGTTTATGTTATTTCCTCATTATAGTATGTCATACGAACACTCGTATGCACAACTTTATTCATGATATTCCAGTACTTTCAGTTATCACAAAATTTACATTTCAATTATAAAAGGTTGTTTAATTTTCCCGTACACATACACTATTCAATTATCTGGCCAATTATGTTCTTTTTAATGTTCCATGCTCTCTTTAATTCTTCAATATTTCTTGATTTGTTTTCTTATTTTCAACATAAACAAAAGAGATTAGAACTCAATCTTTGTTGTTTGAAAAATCATTTTAGTAGATCATGAATAGAGAACAATCATGAGAGAATGGAAAAATACTATAACATTACATCCACGTGTTAAATGGTTTGTTAGAATTGGATTTTATTTTAGTTCTTCTTCTCTGATGGAATATTCTTGTCCATGAGTGATTATCTTTGCAAATTGTCTTATTAATTTTTTTTTTTAACCTGATATTGATTCTTGACGTTAATATAAGTTGCTAACTCAGAAGGACCCAGTAAATAGCCTTCTTGTTCTCCTTATATTTTCTATCTGTCAATGATGTAAATCCAGATATGTTACTATTTCAATTTAATTCGTTAAAGTCATCTGCTACGATCATGTTACTCATCCTTACATTGCTACATTGGTTTTTTTAATCAATTTTTTTAGTATGGACTATGGACATTTGTTGTAGCTAAAAATATTTATTGTATTGCAGAGGCCACTTCATGCATGGAGCAGGCAGTTAATCTATTTTGTGATATTGGAAGGCTCAGTATGGCTGCAAGATATTTGAAGGTACATGTTTATGCTACTTAAACCTCAACTCTTTAGTTTCGCAGGATCATTTAGAAAATTAATTATTCAATAATGTCTTCATTAGCTTTCTTTTTCTTGGTTGAATTTATTTGCTTCTTTTTCTACTTTGAATGTAAGGCATTGTTCAAATTGTAATCATCTTTGGTGATGTAGGAAATTGCTGAACTATATGAGTCTGATCAGAACATTGAACTTGCTATTGGTTACTTTGAGAAAGCTGCAGACTTTTTCCAAAATGAAGAAGTAACAACATCTGCGAACCAGTGCAAGCAGAAAGTGGCACAATTTGCCGCTCAATTAGAACAGTAAGCCTTTCTACTCATCCAATTGTTGTACGCTTCTCTTAATAAATTAATCATGAGTTGTCATTGGCTTAATTCTCAATCATATTTTCCTCTATTTGACAACAGTTTAATGAGGCCATGAGTATGTACATCATGGTTATAAATGTGTACAAGGAAAAACAATATCCTTTATATTTTTCTCAATGATATCCGTATTTCAATGGGAAATATATCTATTATCATTTTAGTATAAATTATAAAACATTTTGTACTATAAAACAAATTTCCGAAAAATTATATTTTTGATATTCTTTCGTCACATTGCTAGAAAATAAATTATAGGATTAGTGTGTATTCTGGTCTAACAGGTGAAAGAATTTTTAGCATGTCCAAAAATCATTACAGATTATACTTAAAAAAAGAATTGTAACAAAACTTCATACAACCCTTTAAATTACGAGGAATGGGATTCCATTTCAGGAGCCTGGCCCTAGGGCTCAACATTAGCTGTCAGGGTATTGGTGATAGATGCTATTGGTAATCTGCTGTGTTTGAGGGTGACTAAATTATTTGTTAGAACAAACGGTAAATATATAGAGAATTGCAATGTATAAATAACCTTGGGTTGGTCTAATGTCAAAAAAGACAACAATTCAAACCTAGATACAAGTCATTGGTTCAAATTATAGTGGTCACCCTCATAAAAGATAACCTATAAATTTCTTGATCTTGATCTTAGATGCTGTAGACAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNAAAATAAAATAAAATTAGTGCAACATATCGATGCCTATTTTGAGAAAAGAAATGATCAAATGCCCTCTATTCCTTTAATTAAAAATTGAAGCCGAATGACGAAAGAGTATGAAACTATTTCTCAACAGTAAAATGTACACAGAAGAGCTTTGTATACGAAGGGAATTTATATTTGAGTTCAAAGCTTCAATAGTGGACTACCTTAGTCGGAAGGTAATAACAGATTATGAACTGTAAATTTTTCTCAGGTACGAAAAGGCAATTAAAATATATGTAGAGATTGCACGGCATTCACTCAAAAGTAACTTGCTAAAGTATGGAGTCAAAGGGCATCTTCTTAATGCTGGCTTATGTCAACTCTGCAAAGGTGATATTGTAGCCATCACCAAAGCATTGGATGAATATCAGGTTTAATTTTGAGACTTTTTGTTTTGTCTTGATGGTTTACATGTACCTTATGGCTACTGTCTTATTCAAATGTCTTGCCTCTTGTTTTACAGGAATTAGATCCAACGTTTTCTAACACACGTGAATGTAGACTTTTAGCTGTAAGTTTGTTTCAATCTTCAACTGATGTTTTTTTTTTCTTTTTTTTTTTCTGCTTCATTCTTGTCTGTATGCACTATGATATAAATTACATGATAGAGGTAGATGACCCTTAATGATCTCTTTTCATTTAGGTTGATTGCAAGTTCAGTTGCAAGTTCAGTTGCATTGACTTCTCCACATGAGTTTTTATTCCATTCTTGCATATTATGTGTGGCTGTCTGCTGGTTTTCAAGATTTCAATTAGATACTTCTTTACATCTTATTTTCATATGAATATAGCATTCTGCATTCTTCTATGACCGCTTTTTTCCCCCCTGGATTTTTTTATCAATCTTGTGCTCTCTCGAGCTGAAAAAGTACTTCATCTGTCAGGATATTGCAAGTGCAATTGATGAAGAAGATGTTGCAAAGTTCACTGACGTCGTTAAGGAGTTCGATAGCATGACCCCTCTGGTAACTCTCTTTGCATTTGCAAAGTTACATTGCATTACAAACTGTAAAGATATCCAATTTGGTTGATAGAAATTCTTAGAGAGACCCTGGTGCTGAAAACTCTCCATGTGTATGTCCATTGCATAGACTAAGGCTATCCATTCTATAATTGCCACACTTCTAATATGTTATATGTTTGGGGATGGATTTATCTTACAATTGCTGGAAAAAGATGTGCGGTGGTGAAAGAGTGCTCTGAGTTTTTTTTTCCCTTGGATGAACTAATTAATCTCCTTGCGTTTTGAAGTTAGAAAAATGCATGCGTTATTGTTTACTTATATGCATAAATTACTTGACCAGGACCCATGGAAGACAACTCTTCTGTTGAGAGTGAAGGAAAAGCTGAAAGCCAAAGAGCTGGAGGAGGATGATCTTACT

mRNA sequence

ATGGCCGATCAGCTTGCCAAGGGAGAGGAATTCGAGAAGAAAGCGGAGAAGAAGCTCAATGGATGGGGATTGTTCGGTTCCAAGTACGAAGATGCAGCTGACCTCTTCGATAAGGCTGCTAACTGTTACAAGCTTTCTAAATCATGGGATAAAGCTGGGTCAACATATACAAAGTTGGCAAGTTGCCATCTGAAGGTGGAAAGTAAGCATGAAGCTGCTCAAGCTTATGTTGATGCTGCCAATTCCTATAAGAAAACATCTATAAAGGAGGCCACTTCATGCATGGAGCAGGCAGTTAATCTATTTTGTGATATTGGAAGGCTCAGTATGGCTGCAAGATATTTGAAGGAAATTGCTGAACTATATGAGTCTGATCAGAACATTGAACTTGCTATTGGTTACTTTGAGAAAGCTGCAGACTTTTTCCAAAATGAAGAAGTAACAACATCTGCGAACCAGTGCAAGCAGAAAGTGGCACAATTTGCCGCTCAATTAGAACAGTACGAAAAGGCAATTAAAATATATGTAGAGATTGCACGGCATTCACTCAAAAGTAACTTGCTAAAGTATGGAGTCAAAGGGCATCTTCTTAATGCTGGCTTATGTCAACTCTGCAAAGGTGATATTGTAGCCATCACCAAAGCATTGGATGAATATCAGGAATTAGATCCAACGTTTTCTAACACACGTGAATGTAGACTTTTAGCTGATATTGCAAGTGCAATTGATGAAGAAGATGTTGCAAAGTTCACTGACGTCGTTAAGGAGTTCGATAGCATGACCCCTCTGGACCCATGGAAGACAACTCTTCTGTTGAGAGTGAAGGAAAAGCTGAAAGCCAAAGAGCTGGAGGAGGATGATCTTACT

Coding sequence (CDS)

ATGGCCGATCAGCTTGCCAAGGGAGAGGAATTCGAGAAGAAAGCGGAGAAGAAGCTCAATGGATGGGGATTGTTCGGTTCCAAGTACGAAGATGCAGCTGACCTCTTCGATAAGGCTGCTAACTGTTACAAGCTTTCTAAATCATGGGATAAAGCTGGGTCAACATATACAAAGTTGGCAAGTTGCCATCTGAAGGTGGAAAGTAAGCATGAAGCTGCTCAAGCTTATGTTGATGCTGCCAATTCCTATAAGAAAACATCTATAAAGGAGGCCACTTCATGCATGGAGCAGGCAGTTAATCTATTTTGTGATATTGGAAGGCTCAGTATGGCTGCAAGATATTTGAAGGAAATTGCTGAACTATATGAGTCTGATCAGAACATTGAACTTGCTATTGGTTACTTTGAGAAAGCTGCAGACTTTTTCCAAAATGAAGAAGTAACAACATCTGCGAACCAGTGCAAGCAGAAAGTGGCACAATTTGCCGCTCAATTAGAACAGTACGAAAAGGCAATTAAAATATATGTAGAGATTGCACGGCATTCACTCAAAAGTAACTTGCTAAAGTATGGAGTCAAAGGGCATCTTCTTAATGCTGGCTTATGTCAACTCTGCAAAGGTGATATTGTAGCCATCACCAAAGCATTGGATGAATATCAGGAATTAGATCCAACGTTTTCTAACACACGTGAATGTAGACTTTTAGCTGATATTGCAAGTGCAATTGATGAAGAAGATGTTGCAAAGTTCACTGACGTCGTTAAGGAGTTCGATAGCATGACCCCTCTGGACCCATGGAAGACAACTCTTCTGTTGAGAGTGAAGGAAAAGCTGAAAGCCAAAGAGCTGGAGGAGGATGATCTTACT

Protein sequence

MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLASCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAELYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIARHSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIASAIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT

Homology

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BLAST of MC04g1838 vs. ExPASy Swiss-Prot
Match: Q9SPE6 (Alpha-soluble NSF attachment protein 2 OS=Arabidopsis thaliana OX=3702 GN=ASNAP2 PE=1 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 5.2e-127
Identity = 224/289 (77.51%), Postives = 252/289 (87.20%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           M D L + EEFEKKAEKKLNGWG+FGSKYEDAADL +KAAN YKL+KSWD+AG  Y KLA
Sbjct: 1   MGDHLVRAEEFEKKAEKKLNGWGIFGSKYEDAADLLEKAANSYKLAKSWDQAGKAYLKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            CHLK +SKH+AA AY +AA  YKK    EA SC+E+AVN+FC+IGRL+MAARY KEIAE
Sbjct: 61  DCHLKSDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNMAARYYKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
            YESDQ  E AI YFEKAA+FFQNEEVTTSANQC  KVAQ+AAQLEQYEKAIKIY +IAR
Sbjct: 121 YYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEKAIKIYEDIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSL +NLLKYGVKGHLL AG+C LCK D+V+IT AL++YQ+LDPTF+ TREC+ LAD+AS
Sbjct: 181 HSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTRECKFLADLAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 290
           AIDEED+AKFTDVVKEFDSMTPLD WKTT+LLRVKEKLKAKELEEDDLT
Sbjct: 241 AIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of MC04g1838 vs. ExPASy Swiss-Prot
Match: P93798 (Alpha-soluble NSF attachment protein OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 2.3e-119
Identity = 215/289 (74.39%), Postives = 246/289 (85.12%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MAD + +GEEFEKKAEKK++GWGLFGSKYEDAAD +DKAANC+KL+KSWD+AGSTY KL+
Sbjct: 1   MADNIQRGEEFEKKAEKKISGWGLFGSKYEDAADFYDKAANCFKLAKSWDRAGSTYVKLS 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           +     +SKHEAAQAY DA + YKKTS KEA SC+EQA  LF D GR +MA +Y KEIAE
Sbjct: 61  TVIQSSDSKHEAAQAYADAGHCYKKTSAKEAISCLEQAAYLFLDNGRFNMAGKYYKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYE +QN E AI YFEKAAD +Q+EE TT+ANQC  KVAQFAAQLEQY+KAI+IY +I R
Sbjct: 121 LYELEQNFEQAIIYFEKAADIYQSEEATTAANQCNAKVAQFAAQLEQYQKAIQIYEDIGR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
            SL +NLLKYGVKGHLLNAG+CQLCKGD+VAIT ALD YQE+DPTFS TRE +LL D+A+
Sbjct: 181 PSLNNNLLKYGVKGHLLNAGICQLCKGDVVAITNALDRYQEMDPTFSGTREYKLLVDLAA 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 290
           A+DEEDV KFTD VKEFDSMT LD WKTTLLLRVKE +KAKELEEDDLT
Sbjct: 241 AVDEEDVVKFTDAVKEFDSMTQLDAWKTTLLLRVKEAIKAKELEEDDLT 289

[Close]

BLAST of MC04g1838 vs. ExPASy Swiss-Prot
Match: Q9M5P8 (Alpha-soluble NSF attachment protein OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 1.1e-113
Identity = 205/289 (70.93%), Postives = 244/289 (84.43%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           M D +A+GE+FE KAEKKL GW LFGSKYEDAADLFDKA NC+KL+KSWDKAG+ Y KLA
Sbjct: 1   MGDHIARGEDFENKAEKKLGGWALFGSKYEDAADLFDKAGNCFKLAKSWDKAGAVYVKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           +CHLK++SKHEAA AY DAA+ YKK++IKEA SC+EQAVN F DIGRL+M+ARY KEIAE
Sbjct: 61  NCHLKLDSKHEAANAYADAAHCYKKSNIKEAVSCLEQAVNFFLDIGRLNMSARYYKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYE +QN++ AI Y     +   +E+V+   NQC +K+AQF+AQ E+Y KAI+I+ EIAR
Sbjct: 121 LYEQEQNLDQAIRYAVYEIN-PGSEDVSAPPNQCLKKIAQFSAQNEKYPKAIEIFEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HS+ +NLLKYGV+ HLLNAG+CQLCKGD+V I  AL+ YQELDPTFS TREC+LL D+A+
Sbjct: 181 HSVNNNLLKYGVREHLLNAGICQLCKGDVVPINNALERYQELDPTFSGTRECKLLVDLAA 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 290
           AIDEEDVAKFTD VKE+DSMT LD W+TTLLLRVKE LKAKELEEDDLT
Sbjct: 241 AIDEEDVAKFTDAVKEYDSMTQLDAWRTTLLLRVKETLKAKELEEDDLT 288

[Close]

BLAST of MC04g1838 vs. ExPASy Swiss-Prot
Match: Q9LXZ5 (Alpha-soluble NSF attachment protein 1 OS=Arabidopsis thaliana OX=3702 GN=ASNAP1 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.1e-67
Identity = 144/253 (56.92%), Postives = 171/253 (67.59%), Query Frame = 0


Query: 11  FEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLASCHLKVESKH 70
           F+KKAEKKLN    F +KYEDAADL +KA + YKL+KSWD+AG  Y KLA CHLK  S  
Sbjct: 99  FQKKAEKKLNRRRRFDTKYEDAADLLEKARDSYKLAKSWDQAGIAYLKLADCHLKANS-- 158

Query: 71  EAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAELYESDQNIEL 130
                    AN+Y          CM+                    EIAE YESD+  E 
Sbjct: 159 --------LANTYMIMD-----ECMDH-------------------EIAEYYESDEMFEQ 218

Query: 131 AIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIARHSLKSNLLKY 190
           AI Y+E AA+FFQ EEVTTSANQC  KVAQ+A+QLEQ  + +K     ARHSL + LLKY
Sbjct: 219 AIAYYETAAEFFQIEEVTTSANQCNLKVAQYASQLEQQSRFMKTQ---ARHSLNNKLLKY 278

Query: 191 GVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIASAIDEEDVAKF 250
           GVKGHLL AG+C LCK D+V+IT AL++YQ+LDPTFS TREC+ LAD+ASAIDEED+AKF
Sbjct: 279 GVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFSGTRECKFLADLASAIDEEDIAKF 314

Query: 251 TDVVKEFDSMTPL 264
           TDV KE DS++PL
Sbjct: 339 TDVSKEIDSVSPL 314

[Close]

BLAST of MC04g1838 vs. ExPASy Swiss-Prot
Match: Q54NP6 (Alpha-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snpA PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.0e-62
Identity = 124/279 (44.44%), Postives = 188/279 (67.38%), Query Frame = 0


Query: 6   AKGEEFEKKAEKKLNGWGLF------GSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKL 65
           AK +EF   A+K+L G   F       S+Y+DAA  + KAAN +K+SK WD+AG+ + K 
Sbjct: 5   AKAKEFLDAADKRLRGGNFFKMFGGGSSRYDDAASDYTKAANLFKMSKKWDQAGAAFQKA 64

Query: 66  ASCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIA 125
           A C LK  SKH+AA +YV AA  YKK ++ +A +C++ A+  + D GR +++A++ KEIA
Sbjct: 65  AECFLKGSSKHDAASSYVLAAGCYKKGNVIDAITCLKAAIEYYTDEGRFAISAKHQKEIA 124

Query: 126 ELYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIA 185
           ELYE++ + + AI  ++ A+D+F  E  T S++QC  K+A F+AQLE+YEK+I+IY ++A
Sbjct: 125 ELYEAEGDFDQAIASYQIASDYFDGENSTVSSHQCLLKIALFSAQLERYEKSIEIYEQVA 184

Query: 186 RHSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIA 245
             SL +NL ++G K + L A LC L   D+V   +AL  Y+++  +F++TRECRLL  I 
Sbjct: 185 AASLDNNLTQWGCKEYFLRACLCYLAADDVVGAERALQRYKDMQASFNSTRECRLLDGII 244

Query: 246 SAIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKL 279
            A    +V  FT+ V EF+S++PLD WKT++LLR+K  +
Sbjct: 245 QACRNNNVEDFTNEVAEFNSISPLDSWKTSILLRIKNTI 283

[Close]

BLAST of MC04g1838 vs. NCBI nr
Match: XP_022134286.1 (alpha-soluble NSF attachment protein 2 [Momordica charantia])

HSP 1 Score: 565 bits (1455), Expect = 9.77e-203
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289
           AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT
Sbjct: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of MC04g1838 vs. NCBI nr
Match: XP_023515361.1 (alpha-soluble NSF attachment protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 541 bits (1393), Expect = 2.76e-193
Identity = 277/289 (95.85%), Postives = 280/289 (96.89%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MADQLAKGEEFEKKA KKLNGWGLFGSKYEDAADLFDKAAN YKL+KSWDKAGSTYTKLA
Sbjct: 1   MADQLAKGEEFEKKAVKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYTKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            CHLKVESKHEAAQAYVDAAN+YKKTSIKEA SCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  GCHLKVESKHEAAQAYVDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYESDQNIELAIGYFEKAAD FQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIY EIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQ+LDPTFS TRECRLLADIA 
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQDLDPTFSGTRECRLLADIAG 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289
           AIDEEDV KFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of MC04g1838 vs. NCBI nr
Match: XP_022939232.1 (alpha-soluble NSF attachment protein 2-like [Cucurbita moschata] >XP_023551462.1 alpha-soluble NSF attachment protein 2-like [Cucurbita pepo subsp. pepo] >KAG6578465.1 Alpha-soluble NSF attachment protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 540 bits (1392), Expect = 3.92e-193
Identity = 276/289 (95.50%), Postives = 282/289 (97.58%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAAN YKL+KSWDKAGSTY KLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SC+LKVESKHEAAQAY DAAN+YKKTSIKEA SCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIY EIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKAL+EYQ+LDPTFS TRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289
           AIDEEDV KFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 289

[Close]

BLAST of MC04g1838 vs. NCBI nr
Match: KAG6602599.1 (Alpha-soluble NSF attachment protein 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033278.1 Alpha-soluble NSF attachment protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 539 bits (1389), Expect = 1.12e-192
Identity = 276/289 (95.50%), Postives = 280/289 (96.89%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MADQLAKGEEFEKKA KKLNGWGLFGSKYEDAADLFDKAAN YKL+KSWDKAGSTYTKLA
Sbjct: 1   MADQLAKGEEFEKKAVKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYTKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            CHLKVESKHEAAQAYVDAAN+YKKTSIKEA SC+EQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  GCHLKVESKHEAAQAYVDAANAYKKTSIKEAISCIEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYESDQNIELAIGYFEKAAD FQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIY EIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQ+LDPTFS TRECRLLADIA 
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQDLDPTFSGTRECRLLADIAG 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289
           AIDEEDV KFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of MC04g1838 vs. NCBI nr
Match: XP_022992586.1 (alpha-soluble NSF attachment protein 2-like [Cucurbita maxima])

HSP 1 Score: 539 bits (1388), Expect = 1.60e-192
Identity = 275/289 (95.16%), Postives = 282/289 (97.58%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAAN YKL+KSWDKAGSTY KLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SC+LKVESKHEAAQAY DAAN+YKKTSIKEA SCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYES+QNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIY EIAR
Sbjct: 121 LYESEQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKAL+EYQ+LDPTFS TRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289
           AIDEEDV KFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 289

[Close]

BLAST of MC04g1838 vs. ExPASy TrEMBL
Match: A0A6J1BXG4 (alpha-soluble NSF attachment protein 2 OS=Momordica charantia OX=3673 GN=LOC111006586 PE=3 SV=1)

HSP 1 Score: 565 bits (1455), Expect = 4.73e-203
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289
           AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT
Sbjct: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of MC04g1838 vs. ExPASy TrEMBL
Match: A0A6J1FG84 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111445208 PE=3 SV=1)

HSP 1 Score: 540 bits (1392), Expect = 1.90e-193
Identity = 276/289 (95.50%), Postives = 282/289 (97.58%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAAN YKL+KSWDKAGSTY KLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SC+LKVESKHEAAQAY DAAN+YKKTSIKEA SCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIY EIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKAL+EYQ+LDPTFS TRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289
           AIDEEDV KFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 289

[Close]

BLAST of MC04g1838 vs. ExPASy TrEMBL
Match: A0A6J1JTZ3 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488888 PE=3 SV=1)

HSP 1 Score: 539 bits (1388), Expect = 7.72e-193
Identity = 275/289 (95.16%), Postives = 282/289 (97.58%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAAN YKL+KSWDKAGSTY KLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SC+LKVESKHEAAQAY DAAN+YKKTSIKEA SCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYES+QNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIY EIAR
Sbjct: 121 LYESEQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKAL+EYQ+LDPTFS TRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289
           AIDEEDV KFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 289

[Close]

BLAST of MC04g1838 vs. ExPASy TrEMBL
Match: A0A6J1JTS3 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487775 PE=3 SV=1)

HSP 1 Score: 538 bits (1385), Expect = 2.21e-192
Identity = 275/289 (95.16%), Postives = 279/289 (96.54%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MADQLAKGEEFEKKA KKLNGWGLFGSKYEDAADLFDK+AN YKL+KSWDKAGSTYTKLA
Sbjct: 1   MADQLAKGEEFEKKAVKKLNGWGLFGSKYEDAADLFDKSANSYKLAKSWDKAGSTYTKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            CHLKVESKHEAAQAYVDAAN+YKKTSIKEA SCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  GCHLKVESKHEAAQAYVDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYESDQNIELAIGYFEKAAD FQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIY EIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQ+LDPTFS TRECRLLAD A 
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQDLDPTFSGTRECRLLADTAG 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289
           AIDEEDV KFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of MC04g1838 vs. ExPASy TrEMBL
Match: A0A6J1HIF0 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464692 PE=3 SV=1)

HSP 1 Score: 536 bits (1380), Expect = 1.28e-191
Identity = 276/289 (95.50%), Postives = 279/289 (96.54%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           MADQLAKGEEFEKKA KKLNGWGLFGSKYEDAADLFDKAAN YKL+KSWDKAGSTYTKLA
Sbjct: 1   MADQLAKGEEFEKKAVKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYTKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            CHLKVESKHEAAQAYVDAAN+YKKTSIKEA SCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  GCHLKVESKHEAAQAYVDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
           LYESDQNIELAIGYFEKAAD FQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIY EIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQ+LDPTFS TRECRLLADIA 
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQDLDPTFSGTRECRLLADIAG 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289
           AIDEEDV KFTDVVKEFDSMTPLDP KTTLLLRVKEKLKAKELEEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPCKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of MC04g1838 vs. TAIR 10
Match: AT3G56190.1 (alpha-soluble NSF attachment protein 2 )

HSP 1 Score: 455.3 bits (1170), Expect = 3.7e-128
Identity = 224/289 (77.51%), Postives = 252/289 (87.20%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60
           M D L + EEFEKKAEKKLNGWG+FGSKYEDAADL +KAAN YKL+KSWD+AG  Y KLA
Sbjct: 1   MGDHLVRAEEFEKKAEKKLNGWGIFGSKYEDAADLLEKAANSYKLAKSWDQAGKAYLKLA 60

Query: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            CHLK +SKH+AA AY +AA  YKK    EA SC+E+AVN+FC+IGRL+MAARY KEIAE
Sbjct: 61  DCHLKSDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNMAARYYKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180
            YESDQ  E AI YFEKAA+FFQNEEVTTSANQC  KVAQ+AAQLEQYEKAIKIY +IAR
Sbjct: 121 YYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEKAIKIYEDIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240
           HSL +NLLKYGVKGHLL AG+C LCK D+V+IT AL++YQ+LDPTF+ TREC+ LAD+AS
Sbjct: 181 HSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTRECKFLADLAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 290
           AIDEED+AKFTDVVKEFDSMTPLD WKTT+LLRVKEKLKAKELEEDDLT
Sbjct: 241 AIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of MC04g1838 vs. TAIR 10
Match: AT3G56190.2 (alpha-soluble NSF attachment protein 2 )

HSP 1 Score: 354.4 bits (908), Expect = 8.8e-98
Identity = 179/239 (74.90%), Postives = 207/239 (86.61%), Query Frame = 0


Query: 53  GSTYTKLA-SCHLKV-ESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSM 112
           GS++  L+ +C L + +SKH+AA AY +AA  YKK    EA SC+E+AVN+FC+IGRL+M
Sbjct: 2   GSSWKSLSKTCRLSLKDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNM 61

Query: 113 AARYLKEIAELYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEK 172
           AARY KEIAE YESDQ  E AI YFEKAA+FFQNEEVTTSANQC  KVAQ+AAQLEQYEK
Sbjct: 62  AARYYKEIAEYYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEK 121

Query: 173 AIKIYVEIARHSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTR 232
           AIKIY +IARHSL +NLLKYGVKGHLL AG+C LCK D+V+IT AL++YQ+LDPTF+ TR
Sbjct: 122 AIKIYEDIARHSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTR 181

Query: 233 ECRLLADIASAIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 290
           EC+ LAD+ASAIDEED+AKFTDVVKEFDSMTPLD WKTT+LLRVKEKLKAKELEEDDLT
Sbjct: 182 ECKFLADLASAIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 240

[Close]

BLAST of MC04g1838 vs. TAIR 10
Match: AT3G56450.1 (alpha-soluble NSF attachment protein 1 )

HSP 1 Score: 257.3 bits (656), Expect = 1.5e-68
Identity = 144/253 (56.92%), Postives = 171/253 (67.59%), Query Frame = 0


Query: 11  FEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLASCHLKVESKH 70
           F+KKAEKKLN    F +KYEDAADL +KA + YKL+KSWD+AG  Y KLA CHLK  S  
Sbjct: 99  FQKKAEKKLNRRRRFDTKYEDAADLLEKARDSYKLAKSWDQAGIAYLKLADCHLKANS-- 158

Query: 71  EAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAELYESDQNIEL 130
                    AN+Y          CM+                    EIAE YESD+  E 
Sbjct: 159 --------LANTYMIMD-----ECMDH-------------------EIAEYYESDEMFEQ 218

Query: 131 AIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIARHSLKSNLLKY 190
           AI Y+E AA+FFQ EEVTTSANQC  KVAQ+A+QLEQ  + +K     ARHSL + LLKY
Sbjct: 219 AIAYYETAAEFFQIEEVTTSANQCNLKVAQYASQLEQQSRFMKTQ---ARHSLNNKLLKY 278

Query: 191 GVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIASAIDEEDVAKF 250
           GVKGHLL AG+C LCK D+V+IT AL++YQ+LDPTFS TREC+ LAD+ASAIDEED+AKF
Sbjct: 279 GVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFSGTRECKFLADLASAIDEEDIAKF 314

Query: 251 TDVVKEFDSMTPL 264
           TDV KE DS++PL
Sbjct: 339 TDVSKEIDSVSPL 314

The following BLAST results are available for this feature:

BLAST of MC04g1838 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SPE6	5.2e-127	77.51	Alpha-soluble NSF attachment protein 2 OS=Arabidopsis thaliana OX=3702 GN=ASNAP2...	[more]
P93798	2.3e-119	74.39	Alpha-soluble NSF attachment protein OS=Vitis vinifera OX=29760 PE=2 SV=1	[more]
Q9M5P8	1.1e-113	70.93	Alpha-soluble NSF attachment protein OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q9LXZ5	2.1e-67	56.92	Alpha-soluble NSF attachment protein 1 OS=Arabidopsis thaliana OX=3702 GN=ASNAP1...	[more]
Q54NP6	2.0e-62	44.44	Alpha-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snp...	[more]

BLAST of MC04g1838 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134286.1	9.77e-203	100.00	alpha-soluble NSF attachment protein 2 [Momordica charantia]	[more]
XP_023515361.1	2.76e-193	95.85	alpha-soluble NSF attachment protein 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022939232.1	3.92e-193	95.50	alpha-soluble NSF attachment protein 2-like [Cucurbita moschata] >XP_023551462.1...	[more]
KAG6602599.1	1.12e-192	95.50	Alpha-soluble NSF attachment protein 2, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022992586.1	1.60e-192	95.16	alpha-soluble NSF attachment protein 2-like [Cucurbita maxima]	[more]

BLAST of MC04g1838 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BXG4	4.73e-203	100.00	alpha-soluble NSF attachment protein 2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1FG84	1.90e-193	95.50	alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1JTZ3	7.72e-193	95.16	alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1JTS3	2.21e-192	95.16	alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1HIF0	1.28e-191	95.50	alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of MC04g1838 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56190.1	3.7e-128	77.51	alpha-soluble NSF attachment protein 2	[more]
AT3G56190.2	8.8e-98	74.90	alpha-soluble NSF attachment protein 2	[more]
AT3G56450.1	1.5e-68	56.92	alpha-soluble NSF attachment protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 151..171
None	No IPR available	PFAM	PF14938	SNAP	coord: 7..278 e-value: 2.2E-110 score: 368.6
None	No IPR available	PANTHER	PTHR13768:SF37	ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN	coord: 1..289
IPR000744	NSF attachment protein	PRINTS	PR00448	NSFATTACHMNT	coord: 224..244 score: 38.78 coord: 255..275 score: 57.82 coord: 152..175 score: 66.07 coord: 28..47 score: 44.29 coord: 183..202 score: 41.43 coord: 91..108 score: 30.95
IPR000744	NSF attachment protein	PANTHER	PTHR13768	SOLUBLE NSF ATTACHMENT PROTEIN SNAP	coord: 1..289
IPR000744	NSF attachment protein	CDD	cd15832	SNAP	coord: 23..278 e-value: 1.5697E-118 score: 339.555
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 5..288 e-value: 3.6E-106 score: 356.6
IPR011990	Tetratricopeptide-like helical domain superfamily	SUPERFAMILY	48452	TPR-like	coord: 7..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g1838.1	MC04g1838.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0035494	SNARE complex disassembly
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005483	soluble NSF attachment protein activity
molecular_function	GO:0019905	syntaxin binding
molecular_function	GO:0005515	protein binding