MC04g1812 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g1812
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein NETWORKED 4A
LocationMC04: 25424275 .. 25425765 (+)
RNA-Seq ExpressionMC04g1812
SyntenyMC04g1812
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCGGAGTGTTGAGCAGATGCTGAAGGCGATCAAAGAAAATAGCCATAATTTCCCGAAAACTGCTGATATGGATTCTCAAGTTGAGGAATTCTCCCGCTTGTATCAATTGTTGGTTGAAAAAGTATTGTCACCGGAGCTACAATTGCAGGTGCCTGTTTATTCTGATTGTGGATCTCCTCAAGGCACTCCGGATTTAAGTTCGGATCAAAAACAGGGTTTCAATATGTCCAGCAATAGAGGCTTGGATATCTCTTTTGATTCTGGTGGTGGTAGCTCCAGTCTTTCTCTGAAGGACGGAACTGAATTTTCTTCTTCTTCTTCATCCGACTCTGAGTCAGAATCTTTTAACTCATCCGTCGACAATAACTATGCGGTTTCACGTGCTGAAAAAGATGGCCAAGGTCTGAAGAAAAAACTTCTTGAGTTAGAAACTGAGCGTCCTAACATGAAAGGGGCATTTTGGGTTGGCGAGGAAGACAAGGTAAATTACGATGAGTTGCAAGACAAATTCGCTAGAATTGAGGAAGAACTAAATGTTTCAAATATAAAGCTTCAGTTATCTGAGAAAGAGGTAGCTAGATTAAAGAGTAAGCTTGAGAAGAACGAAACAATCTTTTTGTTATCGGAAGGATTGCAGGCTCAGCTTGCGTCAGCTGAAAAAGATAAGGAAGAAAGGGAGGCGGACCTTCAAGTGGAAAAAATGCGAGTTCTGGAACTGCAACAACAAACAGAACAGTTGAAAACTCGGGCTTCTGAATCCGATTCCAGAATTGAAAGATTGATTGAAGAGTTAGAAATGAGCAGAGAAATGCTCAGGAATTCAGATGACAGGATCGCAAAGCTGACGCATGAACTAGATATTGTGCAATCTGACCACCATCTTCAGATCAAGGAATTAGAAACAGTTTTTCAAGTTTCACAGGAGCGATTCCATGCTGAGAAAGAACAGATGCAGTCTGATATTCTCAAACAAGTTGAAGCTGAGAAAACAGGAACTAGAGCTCTCCACATTGCTCATGAGACAGCCTTGCAAGGTGAGATTAGTCAGTTGAAAGATGAACTATCTGCAAGAAGCGAAAGTTTGGCAGCTCTGAATAGATACCACGACGAACTAAAATTGAAACACGATACGCTCATGGCCGAGAAAGATGAATTGACCGCCACAGTTCACAAACTTTTAGCTGATGAAGAATCCAGAGAGTCTCGGGTCCATGAATTGGAGGTTCATCTTCAACATTTACAAGTAGAGAAGGAGGGCTTGATTTCTGGATCTGAAAGCCTGAAAAAACAAAATGATGATTTGAAATTGGAACTCTCAGAGTTGCAGGAAGAAGTGAACAAGCAAAAAGTTACGATCGAGGATGGGGCCGAGGGGAAAAGGGAGGCCATAAGGCAGCTCTGTTTCTCTCTGGATCACTACAGGAGTGGGTATCAAGAGCTTCGTCAAGCATTCGTTGGCCACAAACAGCGTCCAGTACTCACTGCG

mRNA sequence

ATGAATCGGAGTGTTGAGCAGATGCTGAAGGCGATCAAAGAAAATAGCCATAATTTCCCGAAAACTGCTGATATGGATTCTCAAGTTGAGGAATTCTCCCGCTTGTATCAATTGTTGGTTGAAAAAGTATTGTCACCGGAGCTACAATTGCAGGTGCCTGTTTATTCTGATTGTGGATCTCCTCAAGGCACTCCGGATTTAAGTTCGGATCAAAAACAGGGTTTCAATATGTCCAGCAATAGAGGCTTGGATATCTCTTTTGATTCTGGTGGTGGTAGCTCCAGTCTTTCTCTGAAGGACGGAACTGAATTTTCTTCTTCTTCTTCATCCGACTCTGAGTCAGAATCTTTTAACTCATCCGTCGACAATAACTATGCGGTTTCACGTGCTGAAAAAGATGGCCAAGGTCTGAAGAAAAAACTTCTTGAGTTAGAAACTGAGCGTCCTAACATGAAAGGGGCATTTTGGGTTGGCGAGGAAGACAAGGTAAATTACGATGAGTTGCAAGACAAATTCGCTAGAATTGAGGAAGAACTAAATGTTTCAAATATAAAGCTTCAGTTATCTGAGAAAGAGGTAGCTAGATTAAAGAGTAAGCTTGAGAAGAACGAAACAATCTTTTTGTTATCGGAAGGATTGCAGGCTCAGCTTGCGTCAGCTGAAAAAGATAAGGAAGAAAGGGAGGCGGACCTTCAAGTGGAAAAAATGCGAGTTCTGGAACTGCAACAACAAACAGAACAGTTGAAAACTCGGGCTTCTGAATCCGATTCCAGAATTGAAAGATTGATTGAAGAGTTAGAAATGAGCAGAGAAATGCTCAGGAATTCAGATGACAGGATCGCAAAGCTGACGCATGAACTAGATATTGTGCAATCTGACCACCATCTTCAGATCAAGGAATTAGAAACAGTTTTTCAAGTTTCACAGGAGCGATTCCATGCTGAGAAAGAACAGATGCAGTCTGATATTCTCAAACAAGTTGAAGCTGAGAAAACAGGAACTAGAGCTCTCCACATTGCTCATGAGACAGCCTTGCAAGGTGAGATTAGTCAGTTGAAAGATGAACTATCTGCAAGAAGCGAAAGTTTGGCAGCTCTGAATAGATACCACGACGAACTAAAATTGAAACACGATACGCTCATGGCCGAGAAAGATGAATTGACCGCCACAGTTCACAAACTTTTAGCTGATGAAGAATCCAGAGAGTCTCGGGTCCATGAATTGGAGGTTCATCTTCAACATTTACAAGTAGAGAAGGAGGGCTTGATTTCTGGATCTGAAAGCCTGAAAAAACAAAATGATGATTTGAAATTGGAACTCTCAGAGTTGCAGGAAGAAGTGAACAAGCAAAAAGTTACGATCGAGGATGGGGCCGAGGGGAAAAGGGAGGCCATAAGGCAGCTCTGTTTCTCTCTGGATCACTACAGGAGTGGGTATCAAGAGCTTCGTCAAGCATTCGTTGGCCACAAACAGCGTCCAGTACTCACTGCG

Coding sequence (CDS)

ATGAATCGGAGTGTTGAGCAGATGCTGAAGGCGATCAAAGAAAATAGCCATAATTTCCCGAAAACTGCTGATATGGATTCTCAAGTTGAGGAATTCTCCCGCTTGTATCAATTGTTGGTTGAAAAAGTATTGTCACCGGAGCTACAATTGCAGGTGCCTGTTTATTCTGATTGTGGATCTCCTCAAGGCACTCCGGATTTAAGTTCGGATCAAAAACAGGGTTTCAATATGTCCAGCAATAGAGGCTTGGATATCTCTTTTGATTCTGGTGGTGGTAGCTCCAGTCTTTCTCTGAAGGACGGAACTGAATTTTCTTCTTCTTCTTCATCCGACTCTGAGTCAGAATCTTTTAACTCATCCGTCGACAATAACTATGCGGTTTCACGTGCTGAAAAAGATGGCCAAGGTCTGAAGAAAAAACTTCTTGAGTTAGAAACTGAGCGTCCTAACATGAAAGGGGCATTTTGGGTTGGCGAGGAAGACAAGGTAAATTACGATGAGTTGCAAGACAAATTCGCTAGAATTGAGGAAGAACTAAATGTTTCAAATATAAAGCTTCAGTTATCTGAGAAAGAGGTAGCTAGATTAAAGAGTAAGCTTGAGAAGAACGAAACAATCTTTTTGTTATCGGAAGGATTGCAGGCTCAGCTTGCGTCAGCTGAAAAAGATAAGGAAGAAAGGGAGGCGGACCTTCAAGTGGAAAAAATGCGAGTTCTGGAACTGCAACAACAAACAGAACAGTTGAAAACTCGGGCTTCTGAATCCGATTCCAGAATTGAAAGATTGATTGAAGAGTTAGAAATGAGCAGAGAAATGCTCAGGAATTCAGATGACAGGATCGCAAAGCTGACGCATGAACTAGATATTGTGCAATCTGACCACCATCTTCAGATCAAGGAATTAGAAACAGTTTTTCAAGTTTCACAGGAGCGATTCCATGCTGAGAAAGAACAGATGCAGTCTGATATTCTCAAACAAGTTGAAGCTGAGAAAACAGGAACTAGAGCTCTCCACATTGCTCATGAGACAGCCTTGCAAGGTGAGATTAGTCAGTTGAAAGATGAACTATCTGCAAGAAGCGAAAGTTTGGCAGCTCTGAATAGATACCACGACGAACTAAAATTGAAACACGATACGCTCATGGCCGAGAAAGATGAATTGACCGCCACAGTTCACAAACTTTTAGCTGATGAAGAATCCAGAGAGTCTCGGGTCCATGAATTGGAGGTTCATCTTCAACATTTACAAGTAGAGAAGGAGGGCTTGATTTCTGGATCTGAAAGCCTGAAAAAACAAAATGATGATTTGAAATTGGAACTCTCAGAGTTGCAGGAAGAAGTGAACAAGCAAAAAGTTACGATCGAGGATGGGGCCGAGGGGAAAAGGGAGGCCATAAGGCAGCTCTGTTTCTCTCTGGATCACTACAGGAGTGGGTATCAAGAGCTTCGTCAAGCATTCGTTGGCCACAAACAGCGTCCAGTACTCACTGCG

Protein sequence

MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGSPQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSSVDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELNVSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLELQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKELETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARSESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKEGLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQELRQAFVGHKQRPVLTA
Homology
BLAST of MC04g1812 vs. ExPASy Swiss-Prot
Match: Q84VY2 (Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 4.9e-24
Identity = 150/522 (28.74%), Postives = 245/522 (46.93%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQ--------VEEFSRLYQLLVEKVLSPELQLQV 60
           M+  V  MLK I+E++ +F K A M  Q        VEEF R+Y+ L E+      +LQ 
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  PVYSDCGSPQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDS 120
              S+  S Q + ++SS  K+  +   +                S K+  E  SSS +DS
Sbjct: 104 NHTSEIQS-QSSLEISSPTKEKLSRRQS----------------SHKE--EEDSSSLTDS 163

Query: 121 ESESFNSSVDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEE--DKVNYDELQD 180
            S+S +SS ++       E   + L +++ ELE E    K    + +E  D  N  +L  
Sbjct: 164 GSDSDHSSAND-------EDGDEALIRRMAELELELQETKQKLLLQQESVDGDNNVDLLH 223

Query: 181 KFARIEEELNVSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREAD 240
           K    E EL  +N K+++ E E+A LK++L+   + F   + L A+  S + DKE+ + D
Sbjct: 224 KITTYEGELKEANEKMRMHEDEIANLKNQLQSFMS-FDTEDHLGAEQKSVDLDKEDTKED 283

Query: 241 LQVEKMRVLELQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIV 300
               K  VL L+++    K +    +     L  ELE+ +     +++++  L HEL++ 
Sbjct: 284 AVATK--VLALEEELSIAKEKLQHFEKETYSLKNELEIGKA----AEEKLKSLQHELELA 343

Query: 301 QSDHHLQIKEL----ETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALH---IAHET 360
           Q D    I +L    + V ++ QER    K  +Q D   ++ A KT            + 
Sbjct: 344 QRDADTYINKLNAEKKEVLKL-QERLAMVKTSLQ-DRDNEIRALKTAVSDAEQKIFPEKA 403

Query: 361 ALQGEISQLKDELSARSESLAALNRYHDELKLKHDTLMAEKDELTATVHKL--LADEES- 420
            ++GE+S++ +E S   E L  L  +   ++L  +     +++L     K+  + DE + 
Sbjct: 404 QIKGEMSKMLEERSQLGEQLRELESH---IRLIKEEKAETEEKLRGGTEKISGMRDESNV 463

Query: 421 -------RESRVHELEVHLQHLQVEKEGLISGSESLKKQNDDLKLELSELQEEVNKQKVT 480
                  RE ++ E E H++ L +E+                L+   SEL EEV + +V+
Sbjct: 464 LREEIGKREEKIKETEKHMEELHMEQV--------------RLRRRSSELTEEVERTRVS 513

Query: 481 IEDGAEGKREAIRQLCFSLDHYRSGYQELRQAFVGHKQRPVL 496
             + AE KREAIRQLC SLDHYR GY  L +   GHK + V+
Sbjct: 524 ASEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVV 513

BLAST of MC04g1812 vs. ExPASy Swiss-Prot
Match: F4KEW8 (Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.6e-22
Identity = 159/539 (29.50%), Postives = 264/539 (48.98%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQ--------VEEFSRLYQLLVEKVL-------- 60
           M+RSV++M+K I+E++ +F K A+M  Q        V+EF R+Y+ L E+          
Sbjct: 44  MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELIALVDEFHRMYRALAERYENITGELRK 103

Query: 61  -SP-ELQLQVPVYSDCGSPQGTPDLSSDQ--KQGFNMSSNR--GLDISFDSGGGSSSLSL 120
            SP ELQ Q    SD  +   +   +S++  + G   S  R  G +    +GG  S L  
Sbjct: 104 GSPLELQSQGSGLSDISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYH 163

Query: 121 KDGTEFSSSSSSDSESESFNSSVDNNYAVSRAEKDGQGLKKKLLELETE--------RPN 180
           KDG +  S+S +DSE ES +SSV N         D Q L K++++LE E        R  
Sbjct: 164 KDGDD--SASITDSELESDDSSVTNYPGYVSIGSDFQSLSKRIMDLEIELREAKERLRMQ 223

Query: 181 MKG---AFWVGEEDKVNYDELQDKFARIEEELNVSNIKLQLSEKEVARLKSKLEKNETIF 240
           ++G   +     + +  + +   K A  E+EL   N KLQ SE ++  LKS+L +     
Sbjct: 224 LEGNTESLLPRVKSETKFVDFPAKLAACEQELKDVNEKLQNSEDQIYILKSQLARYLPSG 283

Query: 241 LLSEGLQAQLASAEKDKEEREADLQVEKMRVLELQQQTEQLKTRASES---DSRIERLIE 300
           L  E  +   ++ E D E    +L++  +R+ E ++Q   ++    +S   D++++ L +
Sbjct: 284 LDDEQSEGAASTQELDIETLSEELRITSLRLREAEKQNGIMRKEVEKSKSDDAKLKSLQD 343

Query: 301 ELEMSR------EMLRNSDDR-IAKLTHELDIVQS-----DHHLQIKELETVFQVSQERF 360
            LE ++      +   ++D R + KL   + +++S     DH  +I++L+T    ++E+ 
Sbjct: 344 MLESAQKEAAAWKSKASADKREVVKLLDRISMLKSSLAGRDH--EIRDLKTALSDAEEKI 403

Query: 361 HAEKEQMQSDILKQVEAEKTGTRALHIAHE-TALQGEISQLKDE-LSARSESLAALNRYH 420
             EK Q+++DI K +E +      +H   +   L+  +  L+DE     +E +       
Sbjct: 404 FPEKAQVKADIAKLLEEK------IHRDDQFKELEANVRYLEDERRKVNNEKI----EEE 463

Query: 421 DELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKEGLISGSESLK 480
           ++LK + + L  EK E    +       E+   +V ELE  +  L         GSE   
Sbjct: 464 EKLKSEIEVLTLEKVEKGRCI-------ETLSRKVSELESEISRL---------GSEI-- 523

Query: 481 KQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQELRQAFVGH 490
           K  DD  +   E+++EV KQ+  +E+ AE KRE IRQLCFSLD+ R  Y+ LR AF GH
Sbjct: 524 KARDDRTM---EMEKEVEKQRRELEEVAEEKREVIRQLCFSLDYSRDEYKRLRIAFSGH 547

BLAST of MC04g1812 vs. NCBI nr
Match: XP_022134362.1 (protein NETWORKED 4B [Momordica charantia])

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS
Sbjct: 32  MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 91

Query: 61  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS 120
           PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS
Sbjct: 92  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS 151

Query: 121 VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN 180
           VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN
Sbjct: 152 VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN 211

Query: 181 VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE 240
           VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE
Sbjct: 212 VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE 271

Query: 241 LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE 300
           LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE
Sbjct: 272 LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE 331

Query: 301 LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS 360
           LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS
Sbjct: 332 LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS 391

Query: 361 ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE 420
           ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE
Sbjct: 392 ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE 451

Query: 421 GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ 480
           GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ
Sbjct: 452 GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ 511

Query: 481 ELRQAFVGHKQRPVLTA 497
           ELRQAFVGHKQRPVLTA
Sbjct: 512 ELRQAFVGHKQRPVLTA 528

BLAST of MC04g1812 vs. NCBI nr
Match: XP_038889573.1 (protein NETWORKED 4A-like [Benincasa hispida] >XP_038889574.1 protein NETWORKED 4A-like [Benincasa hispida])

HSP 1 Score: 686 bits (1771), Expect = 1.44e-243
Identity = 387/499 (77.56%), Postives = 430/499 (86.17%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IKENS NFPK+ADMDSQVEEFSRLYQ LVE VLSPELQLQVPVYSDCGS
Sbjct: 32  MNRSVEQMLKTIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 91

Query: 61  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS 120
           PQGTP+LSSDQKQGFN++SNRGLDISFDSGGGSSSLSLKDGTE SS SSSDSESESFNSS
Sbjct: 92  PQGTPELSSDQKQGFNLTSNRGLDISFDSGGGSSSLSLKDGTE-SSPSSSDSESESFNSS 151

Query: 121 VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEED--KVNYDELQDKFARIEEE 180
           VDNNY VSR+E+D  GLKKKLL +ETE PN K  FWVGEED  KVNYDEL DK A+IEEE
Sbjct: 152 VDNNYVVSRSERDSHGLKKKLLGIETELPNSKRGFWVGEEDNHKVNYDELHDKIAKIEEE 211

Query: 181 LNVSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRV 240
           L VSN KLQ SE EV+RLKS+LEKNET   LSEGLQAQL  AEKDK+E EADLQV+K   
Sbjct: 212 LKVSNAKLQSSENEVSRLKSELEKNETAIWLSEGLQAQLELAEKDKQEMEADLQVKK--- 271

Query: 241 LELQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQI 300
               +Q EQL+TRA +SDS+IERLI+ELE S+EML++S D++ +LTHEL+I +SDHHLQ+
Sbjct: 272 ----RQIEQLETRALQSDSKIERLIQELETSKEMLKSSSDKVTRLTHELEITKSDHHLQV 331

Query: 301 KELETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSA 360
           KELET FQVSQERFHAEKEQMQ+DIL+QVEAEKT T+ALH +H T LQGEISQLK+EL  
Sbjct: 332 KELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETKALHNSHLTVLQGEISQLKEELYV 391

Query: 361 RSESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVE 420
           +S+ LAALN+ HDELKLK+D LMAEKDE +A V+ LLAD+ESRES VHELE  LQHLQVE
Sbjct: 392 KSQGLAALNQNHDELKLKYDMLMAEKDEASAMVNSLLADKESRESHVHELEGDLQHLQVE 451

Query: 421 KEGLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSG 480
           K  LI GSE   KQ DDLKL L EL+EEVNKQK  IEDGAEGKREAIRQLCFSL+HYRSG
Sbjct: 452 KMNLIEGSERKNKQIDDLKLRLLELEEEVNKQKTMIEDGAEGKREAIRQLCFSLEHYRSG 511

Query: 481 YQELRQAFVGHKQRPVLTA 497
           YQELR+AF+GHK RPVL+A
Sbjct: 512 YQELREAFIGHKPRPVLSA 522

BLAST of MC04g1812 vs. NCBI nr
Match: XP_022993014.1 (protein NETWORKED 4A [Cucurbita maxima] >XP_022993015.1 protein NETWORKED 4A [Cucurbita maxima])

HSP 1 Score: 681 bits (1757), Expect = 2.31e-241
Identity = 383/497 (77.06%), Postives = 434/497 (87.32%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IK+NS+NFPK+ADMDSQVEEFSRLYQ L+E +LSPELQ QVP YSDCGS
Sbjct: 32  MNRSVEQMLKTIKDNSNNFPKSADMDSQVEEFSRLYQSLLENLLSPELQPQVPFYSDCGS 91

Query: 61  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS 120
           PQ TP+LSSDQKQGFN+S NRGLDIS DSGGGSSSLSLKDGT  SSSSSSDSESESF+SS
Sbjct: 92  PQCTPELSSDQKQGFNLSCNRGLDISLDSGGGSSSLSLKDGTG-SSSSSSDSESESFSSS 151

Query: 121 VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN 180
           VDNNY VSRAE+DGQG KKK L LETE  N+KGAFWVGEEDK+NYDEL +K ARIEEEL 
Sbjct: 152 VDNNYVVSRAERDGQGRKKKFLGLETELSNIKGAFWVGEEDKLNYDELHEKIARIEEELK 211

Query: 181 VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE 240
           VSN KLQ SE EVARLKS+LEKNET   LSEGLQAQL SAEKDK+E  ADLQVEK RV+E
Sbjct: 212 VSNAKLQSSENEVARLKSELEKNETAMSLSEGLQAQLESAEKDKQEMGADLQVEKRRVVE 271

Query: 241 LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE 300
           L+QQ EQL+TR  +SDS+IE LIEELEMSRE+L++SDD+IA+LTHEL+I +SDH LQIKE
Sbjct: 272 LEQQIEQLETRVLQSDSKIEILIEELEMSREILKSSDDKIARLTHELEITKSDHRLQIKE 331

Query: 301 LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS 360
           LE+ FQVSQE F+AEKEQMQ+D+L+QVEA+KT  RALH +H T+LQGEISQLK+EL  +S
Sbjct: 332 LESAFQVSQEIFNAEKEQMQADVLRQVEADKTEIRALHNSHLTSLQGEISQLKEELFVKS 391

Query: 361 ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE 420
           +SL+ALN  HDELKLK++ LMAEKDE TA VH L+AD+ESRES VHELE HLQHLQ E+E
Sbjct: 392 QSLSALNSNHDELKLKYNMLMAEKDEATAMVHTLVADKESRESHVHELESHLQHLQEEEE 451

Query: 421 GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ 480
           GLI+GSE   KQ DDLKL LSE+QEEVN+Q   IEDGAE KREAIRQLCFSL+HYRSGY+
Sbjct: 452 GLIAGSEIQNKQIDDLKLRLSEMQEEVNRQMNMIEDGAERKREAIRQLCFSLEHYRSGYK 511

Query: 481 ELRQAFVGHKQRPVLTA 497
           ELR+AF+GHK R VL+A
Sbjct: 512 ELREAFIGHKPRQVLSA 527

BLAST of MC04g1812 vs. NCBI nr
Match: KAG6578490.1 (Protein NETWORKED 4A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 672 bits (1735), Expect = 2.65e-237
Identity = 380/497 (76.46%), Postives = 429/497 (86.32%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IK+N  NFPK+ADMDSQVEEFS LYQ L+E +LSPELQ QVP YSDCGS
Sbjct: 78  MNRSVEQMLKTIKDNCDNFPKSADMDSQVEEFSHLYQSLLENLLSPELQPQVPFYSDCGS 137

Query: 61  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS 120
           PQ TP+LSSDQKQGFN+S NRGLDIS DSGGGSSSLSLKDGT  SSSSSSDSESESF+SS
Sbjct: 138 PQCTPELSSDQKQGFNLSCNRGLDISLDSGGGSSSLSLKDGTG-SSSSSSDSESESFSSS 197

Query: 121 VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN 180
           VDNNY VSRAE+DGQG KKK L LETE  N+KGAFWVGEEDK+NYDEL +K ARIEEEL 
Sbjct: 198 VDNNYVVSRAERDGQGPKKKFLGLETELSNIKGAFWVGEEDKLNYDELHEKIARIEEELK 257

Query: 181 VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE 240
           VSN KLQ SE EVARLKS+LEKNET   LSEGLQAQL SAEKDK+E  ADLQVEK RV+E
Sbjct: 258 VSNAKLQSSENEVARLKSELEKNETAMSLSEGLQAQLESAEKDKQEMGADLQVEKRRVVE 317

Query: 241 LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE 300
           L+QQ EQL TR  +SDS+IE LIEELEMSRE+L++SDD+IA+ THEL+I +SDH LQIKE
Sbjct: 318 LEQQIEQLGTRVLQSDSKIEILIEELEMSREILKSSDDKIARFTHELEITKSDHRLQIKE 377

Query: 301 LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS 360
           LE+ FQVSQE F+A KEQ+Q+D+L+QVEA+KT  RALH +H T+LQGEISQLK+EL  +S
Sbjct: 378 LESAFQVSQELFNAGKEQVQADVLRQVEADKTEMRALHNSHLTSLQGEISQLKEELLVKS 437

Query: 361 ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE 420
           +SL+ALN  HDELKLK++ LMAEKDE TA VH L+AD+ESRES VHELE HLQHLQ EKE
Sbjct: 438 QSLSALNSNHDELKLKYNMLMAEKDEATAMVHTLVADKESRESHVHELESHLQHLQEEKE 497

Query: 421 GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ 480
           GLI+GSES  KQ DDLKL LSE+QEEVN+Q   IEDGAE KREAIRQLCFSL+HYRSGY+
Sbjct: 498 GLIAGSESQNKQIDDLKLGLSEMQEEVNRQMNMIEDGAERKREAIRQLCFSLEHYRSGYK 557

Query: 481 ELRQAFVGHKQRPVLTA 497
           ELR+AF+GHK R VL+A
Sbjct: 558 ELREAFIGHKPRQVLSA 573

BLAST of MC04g1812 vs. NCBI nr
Match: XP_022939575.1 (protein NETWORKED 4A-like [Cucurbita moschata] >XP_022939576.1 protein NETWORKED 4A-like [Cucurbita moschata])

HSP 1 Score: 671 bits (1730), Expect = 2.83e-237
Identity = 379/496 (76.41%), Postives = 429/496 (86.49%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IK+N  NFPK+ADMDSQVEEFSRLYQ L+E +LSPELQ QVP YSDCGS
Sbjct: 32  MNRSVEQMLKTIKDNCDNFPKSADMDSQVEEFSRLYQSLLENLLSPELQPQVPFYSDCGS 91

Query: 61  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS 120
           PQ TP+LSSDQKQGFN+S NRGLDIS DSGGGSSSLSLKDGT  SSSSSSDSESESF+SS
Sbjct: 92  PQCTPELSSDQKQGFNLSCNRGLDISLDSGGGSSSLSLKDGTG-SSSSSSDSESESFSSS 151

Query: 121 VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN 180
           VDNNY VSRAE+DGQG KKK L LETE  N++GAFWVGEEDK+NY EL +K ARIEEEL 
Sbjct: 152 VDNNYVVSRAERDGQGPKKKFLGLETELSNIEGAFWVGEEDKLNYHELHEKIARIEEELK 211

Query: 181 VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE 240
           VSN KLQ SE EVARLKS+LEKNET   LSEGLQAQL SAEKDK+E  ADLQV K RV+E
Sbjct: 212 VSNAKLQSSENEVARLKSELEKNETAMSLSEGLQAQLESAEKDKQEMGADLQVLKRRVVE 271

Query: 241 LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE 300
           L+QQ EQL+TR  +SDS+IE LIEELEMSRE+L++SDD+IA+LTHEL+I +SDH LQIKE
Sbjct: 272 LEQQIEQLETRVLQSDSKIEILIEELEMSREILKSSDDKIARLTHELEITKSDHRLQIKE 331

Query: 301 LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS 360
           LE+ FQVSQE F+AEKEQ+Q+D+L+QVEA+KT  RALH +H T+LQGEISQLK+EL  +S
Sbjct: 332 LESAFQVSQELFNAEKEQVQADVLRQVEADKTEMRALHNSHLTSLQGEISQLKEELLVKS 391

Query: 361 ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE 420
           +SL+ALN  HDELKLK+D LMAEKDE TA VH L+AD+E RES VHELE HLQHLQ EKE
Sbjct: 392 QSLSALNSNHDELKLKYDMLMAEKDEATAMVHTLVADKEYRESHVHELESHLQHLQEEKE 451

Query: 421 GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ 480
           GLI+GSES  KQ DDLKL LSE+QEEVN+Q   IEDGAE KREAIRQLCFSL+HYRSGY+
Sbjct: 452 GLIAGSESQNKQIDDLKLGLSEMQEEVNRQMNMIEDGAERKREAIRQLCFSLEHYRSGYK 511

Query: 481 ELRQAFVGHKQRPVLT 496
           ELR+AF+GHK R VL+
Sbjct: 512 ELREAFIGHKPRQVLS 526

BLAST of MC04g1812 vs. ExPASy TrEMBL
Match: A0A6J1BZF1 (protein NETWORKED 4B OS=Momordica charantia OX=3673 GN=LOC111006642 PE=4 SV=1)

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS
Sbjct: 32  MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 91

Query: 61  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS 120
           PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS
Sbjct: 92  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS 151

Query: 121 VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN 180
           VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN
Sbjct: 152 VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN 211

Query: 181 VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE 240
           VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE
Sbjct: 212 VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE 271

Query: 241 LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE 300
           LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE
Sbjct: 272 LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE 331

Query: 301 LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS 360
           LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS
Sbjct: 332 LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS 391

Query: 361 ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE 420
           ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE
Sbjct: 392 ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE 451

Query: 421 GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ 480
           GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ
Sbjct: 452 GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ 511

Query: 481 ELRQAFVGHKQRPVLTA 497
           ELRQAFVGHKQRPVLTA
Sbjct: 512 ELRQAFVGHKQRPVLTA 528

BLAST of MC04g1812 vs. ExPASy TrEMBL
Match: A0A6J1JXC2 (protein NETWORKED 4A OS=Cucurbita maxima OX=3661 GN=LOC111489163 PE=4 SV=1)

HSP 1 Score: 681 bits (1757), Expect = 1.12e-241
Identity = 383/497 (77.06%), Postives = 434/497 (87.32%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IK+NS+NFPK+ADMDSQVEEFSRLYQ L+E +LSPELQ QVP YSDCGS
Sbjct: 32  MNRSVEQMLKTIKDNSNNFPKSADMDSQVEEFSRLYQSLLENLLSPELQPQVPFYSDCGS 91

Query: 61  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS 120
           PQ TP+LSSDQKQGFN+S NRGLDIS DSGGGSSSLSLKDGT  SSSSSSDSESESF+SS
Sbjct: 92  PQCTPELSSDQKQGFNLSCNRGLDISLDSGGGSSSLSLKDGTG-SSSSSSDSESESFSSS 151

Query: 121 VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN 180
           VDNNY VSRAE+DGQG KKK L LETE  N+KGAFWVGEEDK+NYDEL +K ARIEEEL 
Sbjct: 152 VDNNYVVSRAERDGQGRKKKFLGLETELSNIKGAFWVGEEDKLNYDELHEKIARIEEELK 211

Query: 181 VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE 240
           VSN KLQ SE EVARLKS+LEKNET   LSEGLQAQL SAEKDK+E  ADLQVEK RV+E
Sbjct: 212 VSNAKLQSSENEVARLKSELEKNETAMSLSEGLQAQLESAEKDKQEMGADLQVEKRRVVE 271

Query: 241 LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE 300
           L+QQ EQL+TR  +SDS+IE LIEELEMSRE+L++SDD+IA+LTHEL+I +SDH LQIKE
Sbjct: 272 LEQQIEQLETRVLQSDSKIEILIEELEMSREILKSSDDKIARLTHELEITKSDHRLQIKE 331

Query: 301 LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS 360
           LE+ FQVSQE F+AEKEQMQ+D+L+QVEA+KT  RALH +H T+LQGEISQLK+EL  +S
Sbjct: 332 LESAFQVSQEIFNAEKEQMQADVLRQVEADKTEIRALHNSHLTSLQGEISQLKEELFVKS 391

Query: 361 ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE 420
           +SL+ALN  HDELKLK++ LMAEKDE TA VH L+AD+ESRES VHELE HLQHLQ E+E
Sbjct: 392 QSLSALNSNHDELKLKYNMLMAEKDEATAMVHTLVADKESRESHVHELESHLQHLQEEEE 451

Query: 421 GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ 480
           GLI+GSE   KQ DDLKL LSE+QEEVN+Q   IEDGAE KREAIRQLCFSL+HYRSGY+
Sbjct: 452 GLIAGSEIQNKQIDDLKLRLSEMQEEVNRQMNMIEDGAERKREAIRQLCFSLEHYRSGYK 511

Query: 481 ELRQAFVGHKQRPVLTA 497
           ELR+AF+GHK R VL+A
Sbjct: 512 ELREAFIGHKPRQVLSA 527

BLAST of MC04g1812 vs. ExPASy TrEMBL
Match: A0A6J1FN40 (protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111445431 PE=4 SV=1)

HSP 1 Score: 671 bits (1730), Expect = 1.37e-237
Identity = 379/496 (76.41%), Postives = 429/496 (86.49%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IK+N  NFPK+ADMDSQVEEFSRLYQ L+E +LSPELQ QVP YSDCGS
Sbjct: 32  MNRSVEQMLKTIKDNCDNFPKSADMDSQVEEFSRLYQSLLENLLSPELQPQVPFYSDCGS 91

Query: 61  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSS 120
           PQ TP+LSSDQKQGFN+S NRGLDIS DSGGGSSSLSLKDGT  SSSSSSDSESESF+SS
Sbjct: 92  PQCTPELSSDQKQGFNLSCNRGLDISLDSGGGSSSLSLKDGTG-SSSSSSDSESESFSSS 151

Query: 121 VDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELN 180
           VDNNY VSRAE+DGQG KKK L LETE  N++GAFWVGEEDK+NY EL +K ARIEEEL 
Sbjct: 152 VDNNYVVSRAERDGQGPKKKFLGLETELSNIEGAFWVGEEDKLNYHELHEKIARIEEELK 211

Query: 181 VSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLE 240
           VSN KLQ SE EVARLKS+LEKNET   LSEGLQAQL SAEKDK+E  ADLQV K RV+E
Sbjct: 212 VSNAKLQSSENEVARLKSELEKNETAMSLSEGLQAQLESAEKDKQEMGADLQVLKRRVVE 271

Query: 241 LQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKE 300
           L+QQ EQL+TR  +SDS+IE LIEELEMSRE+L++SDD+IA+LTHEL+I +SDH LQIKE
Sbjct: 272 LEQQIEQLETRVLQSDSKIEILIEELEMSREILKSSDDKIARLTHELEITKSDHRLQIKE 331

Query: 301 LETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARS 360
           LE+ FQVSQE F+AEKEQ+Q+D+L+QVEA+KT  RALH +H T+LQGEISQLK+EL  +S
Sbjct: 332 LESAFQVSQELFNAEKEQVQADVLRQVEADKTEMRALHNSHLTSLQGEISQLKEELLVKS 391

Query: 361 ESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKE 420
           +SL+ALN  HDELKLK+D LMAEKDE TA VH L+AD+E RES VHELE HLQHLQ EKE
Sbjct: 392 QSLSALNSNHDELKLKYDMLMAEKDEATAMVHTLVADKEYRESHVHELESHLQHLQEEKE 451

Query: 421 GLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQ 480
           GLI+GSES  KQ DDLKL LSE+QEEVN+Q   IEDGAE KREAIRQLCFSL+HYRSGY+
Sbjct: 452 GLIAGSESQNKQIDDLKLGLSEMQEEVNRQMNMIEDGAERKREAIRQLCFSLEHYRSGYK 511

Query: 481 ELRQAFVGHKQRPVLT 496
           ELR+AF+GHK R VL+
Sbjct: 512 ELREAFIGHKPRQVLS 526

BLAST of MC04g1812 vs. ExPASy TrEMBL
Match: A0A1S3CAE2 (protein NETWORKED 4A OS=Cucumis melo OX=3656 GN=LOC103498608 PE=4 SV=1)

HSP 1 Score: 668 bits (1724), Expect = 9.69e-237
Identity = 377/497 (75.86%), Postives = 426/497 (85.71%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IKENS +FPK+ADMDSQVEEFSRLYQ LVE VLSPELQLQVPVYSDCGS
Sbjct: 32  MNRSVEQMLKTIKENSDSFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 91

Query: 61  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSS-DSESESFNS 120
           PQGTP+LSSDQKQGFN+SSNRGLDISFDSGGGSSSLSLKDGTE SSSSSS DSESESFNS
Sbjct: 92  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 151

Query: 121 SVDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEEL 180
           SVDNNY VSRAE+DGQGLKKKLL +E+E PN+KG FWVGEE KVNYDEL DK A++EEEL
Sbjct: 152 SVDNNYVVSRAERDGQGLKKKLLAIESELPNIKGGFWVGEEVKVNYDELHDKIAKMEEEL 211

Query: 181 NVSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVL 240
            VSN KLQ SE EV RLKS++EKNET  LLSEGLQAQL SAEKDK+  EA+LQV+K    
Sbjct: 212 KVSNAKLQSSENEVTRLKSEVEKNETAILLSEGLQAQLESAEKDKQVMEAELQVKK---- 271

Query: 241 ELQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIK 300
              +Q EQL+TR   SDS+IERLI+ELE+S+EML +S+D+I +LTHEL+I +SDHH QIK
Sbjct: 272 ---KQIEQLETRILLSDSKIERLIKELEISKEMLNSSNDKITRLTHELEITKSDHHKQIK 331

Query: 301 ELETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSAR 360
           ELET FQVSQERFHAEKEQ+++DIL+QVEAEK  T+ALH +  +  QGEISQLK+ELS R
Sbjct: 332 ELETAFQVSQERFHAEKEQLETDILRQVEAEKAETKALHYSQLSMYQGEISQLKEELSVR 391

Query: 361 SESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEK 420
           SE+LA LNR HDELKLK+D +MAEKDE +A V  LLAD+ESRES VHELE  L+HLQVEK
Sbjct: 392 SENLADLNRNHDELKLKYDMVMAEKDEASAVVLSLLADKESRESHVHELEGQLKHLQVEK 451

Query: 421 EGLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGY 480
             LI GSE   K+ DDLKL L+EL+EEV  QK  IEDGAEGKREAIRQLCFSL+HYR+GY
Sbjct: 452 TRLIEGSERQNKEIDDLKLRLAELEEEVKMQKSMIEDGAEGKREAIRQLCFSLEHYRNGY 511

Query: 481 QELRQAFVGHKQRPVLT 496
           QELR+AF+ HK RPVL+
Sbjct: 512 QELREAFIAHKPRPVLS 521

BLAST of MC04g1812 vs. ExPASy TrEMBL
Match: A0A0A0KXR2 (NAB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G645160 PE=4 SV=1)

HSP 1 Score: 668 bits (1723), Expect = 3.15e-235
Identity = 375/498 (75.30%), Postives = 427/498 (85.74%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQVEEFSRLYQLLVEKVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IKENS +FP++ DMDSQVEEFSRLYQ LVE VLSPELQLQVPVYSDCGS
Sbjct: 120 MNRSVEQMLKTIKENSDSFPESVDMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 179

Query: 61  PQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSS-DSESESFNS 120
           PQGTP+LSSDQKQGFN+SSNRGLDISFDSGGGSSSLSLKDGTE SSSSSS DSESESFNS
Sbjct: 180 PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 239

Query: 121 SVDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEEL 180
           SVDNNY VSRAE+DGQGLKKKLL +E+E PN+KG FWVGEE KVNYDEL DK A+IEEEL
Sbjct: 240 SVDNNYVVSRAERDGQGLKKKLLAIESELPNIKGGFWVGEEVKVNYDELHDKIAKIEEEL 299

Query: 181 NVSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVL 240
            VSN KLQ SE EV RLKS++EKNET  LLSEGLQAQL S EKDK+  E +LQV+K ++ 
Sbjct: 300 KVSNAKLQSSENEVTRLKSEVEKNETAILLSEGLQAQLESVEKDKQVMETELQVKKKKI- 359

Query: 241 ELQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIK 300
                 EQL+TR S+SDS+IERLI++LE+S+EML++S+D+I +LTHEL+  +SDHH+QIK
Sbjct: 360 ------EQLETRISQSDSKIERLIKDLEISKEMLKSSNDKITRLTHELENTKSDHHIQIK 419

Query: 301 ELETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSAR 360
           ELET FQVSQERFHAEKEQM++DIL+QVEAEKT T+ALH +  T  QGEISQLK+ELS R
Sbjct: 420 ELETAFQVSQERFHAEKEQMETDILRQVEAEKTETKALHNSQLTMYQGEISQLKEELSVR 479

Query: 361 SESLAALNRYHDELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEK 420
           SESLA LNR HDELKLK+D +MAEKDE +A V  LLAD+ESRES V+EL+  L+HLQV+K
Sbjct: 480 SESLADLNRNHDELKLKYDMVMAEKDEASAVVLSLLADKESRESHVNELKGQLKHLQVDK 539

Query: 421 EGLISGSESLKKQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGY 480
             LI GSE   K+ DDLKL LSEL EEV  QK  IEDGAEGKREAIRQLCFSL+HYR+GY
Sbjct: 540 RRLIEGSERQNKEIDDLKLRLSELGEEVKMQKTMIEDGAEGKREAIRQLCFSLEHYRNGY 599

Query: 481 QELRQAFVGHKQRPVLTA 497
           QELR+AF+ HK RPVL+A
Sbjct: 600 QELREAFIAHKPRPVLSA 610

BLAST of MC04g1812 vs. TAIR 10
Match: AT5G05180.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G10880.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 155.2 bits (391), Expect = 1.4e-37
Identity = 143/439 (32.57%), Postives = 228/439 (51.94%), Query Frame = 0

Query: 78  SSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSSVDNNYAVSRAEKDGQGL 137
           +SN  +DIS D  G S  ++ K  T    +S+ D  S   + + D+N + S  + D +  
Sbjct: 15  NSNSFVDISTDHNGSSPPVTPKSDT---VASNFDQGSSDVSGNHDSNSSYSDLDSDTEAF 74

Query: 138 KKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELNVSNIKLQLSEKEVARLK 197
                      P    +  +  E +++Y+EL  K+ + EEEL  +++KLQ  E+E+ +LK
Sbjct: 75  YSS-FNHHLVSPGSMDSHDLSPEKQMSYEELMKKYVQCEEELRTTSLKLQEFEQEIEKLK 134

Query: 198 SKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLELQQQTEQLKTRASESDS 257
            + EK E++ L  E L+ +   A+ +   R+  ++ E+ RVLE+Q+Q   L+T  S+   
Sbjct: 135 -ETEKKESVVLFGEYLRGEREIAQGEIAIRDIAIETERKRVLEVQRQVVDLETELSDLSF 194

Query: 258 RIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKELETVFQVSQERFHAEKE 317
           + E L+ E E+SR+ L  S   I+KL   L       +  I++ + V Q+     H+E E
Sbjct: 195 KFEHLVNEHEVSRDCLDVSFSEISKLREML--CDCQQNFSIEKTKLVDQIK----HSEAE 254

Query: 318 QMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARSESLAALNRYHDELKLKH 377
           +M+    K+VE                LQ EIS LK +L+ R E + ALN+  D+ KL++
Sbjct: 255 KMEMQ-RKEVE----------------LQAEISALKTDLATRGEHIEALNKDFDKHKLRY 314

Query: 378 DTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKEGLISGSESLKKQNDDLK 437
           D LMAEKD + A V  L A+  SR+ ++ ++E  L  L  ++  L+S S + K   ++LK
Sbjct: 315 DMLMAEKDGVCAEVDNLKAEMRSRDIQIQQMEEQLNQLVYKQTELVSESGNAKNTVEELK 374

Query: 438 LELSELQEEV---NKQKVTIED---------------------GAEGKREAIRQLCFSLD 493
             + EL+ EV   +K K T+E+                     G E KREAIRQLCFSLD
Sbjct: 375 AVVKELEIEVELQSKAKKTVEELRATVWEMEKHAELQRNAISQGEEEKREAIRQLCFSLD 425

BLAST of MC04g1812 vs. TAIR 10
Match: AT5G05180.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G10880.1); Has 50151 Blast hits to 28081 proteins in 1838 species: Archae - 860; Bacteria - 6013; Metazoa - 25867; Fungi - 3836; Plants - 2236; Viruses - 90; Other Eukaryotes - 11249 (source: NCBI BLink). )

HSP 1 Score: 137.9 bits (346), Expect = 2.2e-32
Identity = 136/439 (30.98%), Postives = 218/439 (49.66%), Query Frame = 0

Query: 78  SSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDSESESFNSSVDNNYAVSRAEKDGQGL 137
           +SN  +DIS D  G S  ++ K  T    +S+ D  S   + + D+N + S  + D +  
Sbjct: 15  NSNSFVDISTDHNGSSPPVTPKSDT---VASNFDQGSSDVSGNHDSNSSYSDLDSDTEAF 74

Query: 138 KKKLLELETERPNMKGAFWVGEEDKVNYDELQDKFARIEEELNVSNIKLQLSEKEVARLK 197
                      P    +  +  E +++Y+EL  K+ + EEEL  +++KLQ  E+E+ +LK
Sbjct: 75  YSS-FNHHLVSPGSMDSHDLSPEKQMSYEELMKKYVQCEEELRTTSLKLQEFEQEIEKLK 134

Query: 198 SKLEKNETIFLLSEGLQAQLASAEKDKEEREADLQVEKMRVLELQQQTEQLKTRASESDS 257
            + EK E++ L  E L+ +   A+ +   R+  ++ E+ RVLE+Q+Q   L+T  S+   
Sbjct: 135 -ETEKKESVVLFGEYLRGEREIAQGEIAIRDIAIETERKRVLEVQRQVVDLETELSDLSF 194

Query: 258 RIERLIEELEMSREMLRNSDDRIAKLTHELDIVQSDHHLQIKELETVFQVSQERFHAEKE 317
           + E L+ E                   HE        +  I++ + V Q+     H+E E
Sbjct: 195 KFEHLVNE-------------------HE-------QNFSIEKTKLVDQIK----HSEAE 254

Query: 318 QMQSDILKQVEAEKTGTRALHIAHETALQGEISQLKDELSARSESLAALNRYHDELKLKH 377
           +M+    K+VE                LQ EIS LK +L+ R E + ALN+  D+ KL++
Sbjct: 255 KMEMQ-RKEVE----------------LQAEISALKTDLATRGEHIEALNKDFDKHKLRY 314

Query: 378 DTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKEGLISGSESLKKQNDDLK 437
           D LMAEKD + A V  L A+  SR+ ++ ++E  L  L  ++  L+S S + K   ++LK
Sbjct: 315 DMLMAEKDGVCAEVDNLKAEMRSRDIQIQQMEEQLNQLVYKQTELVSESGNAKNTVEELK 374

Query: 438 LELSELQEEV---NKQKVTIED---------------------GAEGKREAIRQLCFSLD 493
             + EL+ EV   +K K T+E+                     G E KREAIRQLCFSLD
Sbjct: 375 AVVKELEIEVELQSKAKKTVEELRATVWEMEKHAELQRNAISQGEEEKREAIRQLCFSLD 401

BLAST of MC04g1812 vs. TAIR 10
Match: AT2G30500.1 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 114.0 bits (284), Expect = 3.5e-25
Identity = 150/522 (28.74%), Postives = 245/522 (46.93%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQ--------VEEFSRLYQLLVEKVLSPELQLQV 60
           M+  V  MLK I+E++ +F K A M  Q        VEEF R+Y+ L E+      +LQ 
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  PVYSDCGSPQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDS 120
              S+  S Q + ++SS  K+  +   +                S K+  E  SSS +DS
Sbjct: 104 NHTSEIQS-QSSLEISSPTKEKLSRRQS----------------SHKE--EEDSSSLTDS 163

Query: 121 ESESFNSSVDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEE--DKVNYDELQD 180
            S+S +SS ++       E   + L +++ ELE E    K    + +E  D  N  +L  
Sbjct: 164 GSDSDHSSAND-------EDGDEALIRRMAELELELQETKQKLLLQQESVDGDNNVDLLH 223

Query: 181 KFARIEEELNVSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREAD 240
           K    E EL  +N K+++ E E+A LK++L+   + F   + L A+  S + DKE+ + D
Sbjct: 224 KITTYEGELKEANEKMRMHEDEIANLKNQLQSFMS-FDTEDHLGAEQKSVDLDKEDTKED 283

Query: 241 LQVEKMRVLELQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIV 300
               K  VL L+++    K +    +     L  ELE+ +     +++++  L HEL++ 
Sbjct: 284 AVATK--VLALEEELSIAKEKLQHFEKETYSLKNELEIGKA----AEEKLKSLQHELELA 343

Query: 301 QSDHHLQIKEL----ETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALH---IAHET 360
           Q D    I +L    + V ++ QER    K  +Q D   ++ A KT            + 
Sbjct: 344 QRDADTYINKLNAEKKEVLKL-QERLAMVKTSLQ-DRDNEIRALKTAVSDAEQKIFPEKA 403

Query: 361 ALQGEISQLKDELSARSESLAALNRYHDELKLKHDTLMAEKDELTATVHKL--LADEES- 420
            ++GE+S++ +E S   E L  L  +   ++L  +     +++L     K+  + DE + 
Sbjct: 404 QIKGEMSKMLEERSQLGEQLRELESH---IRLIKEEKAETEEKLRGGTEKISGMRDESNV 463

Query: 421 -------RESRVHELEVHLQHLQVEKEGLISGSESLKKQNDDLKLELSELQEEVNKQKVT 480
                  RE ++ E E H++ L +E+                L+   SEL EEV + +V+
Sbjct: 464 LREEIGKREEKIKETEKHMEELHMEQV--------------RLRRRSSELTEEVERTRVS 513

Query: 481 IEDGAEGKREAIRQLCFSLDHYRSGYQELRQAFVGHKQRPVL 496
             + AE KREAIRQLC SLDHYR GY  L +   GHK + V+
Sbjct: 524 ASEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVV 513

BLAST of MC04g1812 vs. TAIR 10
Match: AT2G30500.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 114.0 bits (284), Expect = 3.5e-25
Identity = 150/522 (28.74%), Postives = 245/522 (46.93%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQ--------VEEFSRLYQLLVEKVLSPELQLQV 60
           M+  V  MLK I+E++ +F K A M  Q        VEEF R+Y+ L E+      +LQ 
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  PVYSDCGSPQGTPDLSSDQKQGFNMSSNRGLDISFDSGGGSSSLSLKDGTEFSSSSSSDS 120
              S+  S Q + ++SS  K+  +   +                S K+  E  SSS +DS
Sbjct: 104 NHTSEIQS-QSSLEISSPTKEKLSRRQS----------------SHKE--EEDSSSLTDS 163

Query: 121 ESESFNSSVDNNYAVSRAEKDGQGLKKKLLELETERPNMKGAFWVGEE--DKVNYDELQD 180
            S+S +SS ++       E   + L +++ ELE E    K    + +E  D  N  +L  
Sbjct: 164 GSDSDHSSAND-------EDGDEALIRRMAELELELQETKQKLLLQQESVDGDNNVDLLH 223

Query: 181 KFARIEEELNVSNIKLQLSEKEVARLKSKLEKNETIFLLSEGLQAQLASAEKDKEEREAD 240
           K    E EL  +N K+++ E E+A LK++L+   + F   + L A+  S + DKE+ + D
Sbjct: 224 KITTYEGELKEANEKMRMHEDEIANLKNQLQSFMS-FDTEDHLGAEQKSVDLDKEDTKED 283

Query: 241 LQVEKMRVLELQQQTEQLKTRASESDSRIERLIEELEMSREMLRNSDDRIAKLTHELDIV 300
               K  VL L+++    K +    +     L  ELE+ +     +++++  L HEL++ 
Sbjct: 284 AVATK--VLALEEELSIAKEKLQHFEKETYSLKNELEIGKA----AEEKLKSLQHELELA 343

Query: 301 QSDHHLQIKEL----ETVFQVSQERFHAEKEQMQSDILKQVEAEKTGTRALH---IAHET 360
           Q D    I +L    + V ++ QER    K  +Q D   ++ A KT            + 
Sbjct: 344 QRDADTYINKLNAEKKEVLKL-QERLAMVKTSLQ-DRDNEIRALKTAVSDAEQKIFPEKA 403

Query: 361 ALQGEISQLKDELSARSESLAALNRYHDELKLKHDTLMAEKDELTATVHKL--LADEES- 420
            ++GE+S++ +E S   E L  L  +   ++L  +     +++L     K+  + DE + 
Sbjct: 404 QIKGEMSKMLEERSQLGEQLRELESH---IRLIKEEKAETEEKLRGGTEKISGMRDESNV 463

Query: 421 -------RESRVHELEVHLQHLQVEKEGLISGSESLKKQNDDLKLELSELQEEVNKQKVT 480
                  RE ++ E E H++ L +E+                L+   SEL EEV + +V+
Sbjct: 464 LREEIGKREEKIKETEKHMEELHMEQV--------------RLRRRSSELTEEVERTRVS 513

Query: 481 IEDGAEGKREAIRQLCFSLDHYRSGYQELRQAFVGHKQRPVL 496
             + AE KREAIRQLC SLDHYR GY  L +   GHK + V+
Sbjct: 524 ASEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVV 513

BLAST of MC04g1812 vs. TAIR 10
Match: AT5G58320.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 109.0 bits (271), Expect = 1.1e-23
Identity = 159/539 (29.50%), Postives = 264/539 (48.98%), Query Frame = 0

Query: 1   MNRSVEQMLKAIKENSHNFPKTADMDSQ--------VEEFSRLYQLLVEKVL-------- 60
           M+RSV++M+K I+E++ +F K A+M  Q        V+EF R+Y+ L E+          
Sbjct: 44  MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELIALVDEFHRMYRALAERYENITGELRK 103

Query: 61  -SP-ELQLQVPVYSDCGSPQGTPDLSSDQ--KQGFNMSSNR--GLDISFDSGGGSSSLSL 120
            SP ELQ Q    SD  +   +   +S++  + G   S  R  G +    +GG  S L  
Sbjct: 104 GSPLELQSQGSGLSDISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYH 163

Query: 121 KDGTEFSSSSSSDSESESFNSSVDNNYAVSRAEKDGQGLKKKLLELETE--------RPN 180
           KDG +  S+S +DSE ES +SSV N         D Q L K++++LE E        R  
Sbjct: 164 KDGDD--SASITDSELESDDSSVTNYPGYVSIGSDFQSLSKRIMDLEIELREAKERLRMQ 223

Query: 181 MKG---AFWVGEEDKVNYDELQDKFARIEEELNVSNIKLQLSEKEVARLKSKLEKNETIF 240
           ++G   +     + +  + +   K A  E+EL   N KLQ SE ++  LKS+L +     
Sbjct: 224 LEGNTESLLPRVKSETKFVDFPAKLAACEQELKDVNEKLQNSEDQIYILKSQLARYLPSG 283

Query: 241 LLSEGLQAQLASAEKDKEEREADLQVEKMRVLELQQQTEQLKTRASES---DSRIERLIE 300
           L  E  +   ++ E D E    +L++  +R+ E ++Q   ++    +S   D++++ L +
Sbjct: 284 LDDEQSEGAASTQELDIETLSEELRITSLRLREAEKQNGIMRKEVEKSKSDDAKLKSLQD 343

Query: 301 ELEMSR------EMLRNSDDR-IAKLTHELDIVQS-----DHHLQIKELETVFQVSQERF 360
            LE ++      +   ++D R + KL   + +++S     DH  +I++L+T    ++E+ 
Sbjct: 344 MLESAQKEAAAWKSKASADKREVVKLLDRISMLKSSLAGRDH--EIRDLKTALSDAEEKI 403

Query: 361 HAEKEQMQSDILKQVEAEKTGTRALHIAHE-TALQGEISQLKDE-LSARSESLAALNRYH 420
             EK Q+++DI K +E +      +H   +   L+  +  L+DE     +E +       
Sbjct: 404 FPEKAQVKADIAKLLEEK------IHRDDQFKELEANVRYLEDERRKVNNEKI----EEE 463

Query: 421 DELKLKHDTLMAEKDELTATVHKLLADEESRESRVHELEVHLQHLQVEKEGLISGSESLK 480
           ++LK + + L  EK E    +       E+   +V ELE  +  L         GSE   
Sbjct: 464 EKLKSEIEVLTLEKVEKGRCI-------ETLSRKVSELESEISRL---------GSEI-- 523

Query: 481 KQNDDLKLELSELQEEVNKQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQELRQAFVGH 490
           K  DD  +   E+++EV KQ+  +E+ AE KRE IRQLCFSLD+ R  Y+ LR AF GH
Sbjct: 524 KARDDRTM---EMEKEVEKQRRELEEVAEEKREVIRQLCFSLDYSRDEYKRLRIAFSGH 547

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84VY24.9e-2428.74Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1[more]
F4KEW81.6e-2229.50Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022134362.10.0100.00protein NETWORKED 4B [Momordica charantia][more]
XP_038889573.11.44e-24377.56protein NETWORKED 4A-like [Benincasa hispida] >XP_038889574.1 protein NETWORKED ... [more]
XP_022993014.12.31e-24177.06protein NETWORKED 4A [Cucurbita maxima] >XP_022993015.1 protein NETWORKED 4A [Cu... [more]
KAG6578490.12.65e-23776.46Protein NETWORKED 4A, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022939575.12.83e-23776.41protein NETWORKED 4A-like [Cucurbita moschata] >XP_022939576.1 protein NETWORKED... [more]
Match NameE-valueIdentityDescription
A0A6J1BZF10.0100.00protein NETWORKED 4B OS=Momordica charantia OX=3673 GN=LOC111006642 PE=4 SV=1[more]
A0A6J1JXC21.12e-24177.06protein NETWORKED 4A OS=Cucurbita maxima OX=3661 GN=LOC111489163 PE=4 SV=1[more]
A0A6J1FN401.37e-23776.41protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111445431 PE=4 SV=... [more]
A0A1S3CAE29.69e-23775.86protein NETWORKED 4A OS=Cucumis melo OX=3656 GN=LOC103498608 PE=4 SV=1[more]
A0A0A0KXR23.15e-23575.30NAB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G645160 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT5G05180.11.4e-3732.57unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G05180.22.2e-3230.98unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT2G30500.13.5e-2528.74Kinase interacting (KIP1-like) family protein [more]
AT2G30500.23.5e-2528.74Kinase interacting (KIP1-like) family protein [more]
AT5G58320.21.1e-2329.50Kinase interacting (KIP1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 158..206
NoneNo IPR availableCOILSCoilCoilcoord: 426..453
NoneNo IPR availableCOILSCoilCoilcoord: 210..279
NoneNo IPR availableCOILSCoilCoilcoord: 127..147
NoneNo IPR availableCOILSCoilCoilcoord: 370..390
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..123
NoneNo IPR availablePANTHERPTHR32258PROTEIN NETWORKED 4Acoord: 2..322
coord: 323..496
NoneNo IPR availablePANTHERPTHR32258:SF14TROPOMYOSINcoord: 2..322
coord: 323..496

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g1812.1MC04g1812.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003779 actin binding