MC04g1779 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g1779 vs. ExPASy Swiss-Prot
Match: P92983 (Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 PE=1 SV=2)

HSP 1 Score: 505.0 bits (1299), Expect = 9.7e-142
Identity = 260/469 (55.44%), Postives = 344/469 (73.35%), Query Frame = 0

Query: 24  PLNSSAAASSPAAAAF--SAADSPL-HLTTTPSATPNIDFADTRALFGSVSTPALLRAAA 83
           P  +++ A  P   +F   A + PL H   T  +   +D +D   LF S+ T  LLR+ A
Sbjct: 26  PTVTASTAVVPEILSFGQQAPEPPLHHPKPTEQSHDGLDLSDQARLFSSIPTSDLLRSTA 85

Query: 84  NLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGAVRHSFYQNFCAGEDYAAVGNTVRRL 143
            LH AA+GP+VD+G WVM+S+LM+  V R  VLG V+ +FY +FCAGED  A    VR +
Sbjct: 86  VLHAAAIGPMVDLGTWVMSSKLMDASVTRGMVLGLVKSTFYDHFCAGEDADAAAERVRSV 145

Query: 144 NDA-GLRSMLDYALEYADDDVACDQNLDGFLRTIESTRSLPPASASFVVAKVSAICPLRL 203
            +A GL+ ML Y +E+ADD V+CD N+  F+RTIE+ +SLP +  S VV K++AICP+ L
Sbjct: 146 YEATGLKGMLVYGVEHADDAVSCDDNMQQFIRTIEAAKSLPTSHFSSVVVKITAICPISL 205

Query: 204 LERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHTLHQPNPLTPQEEQSLQISHQRL 263
           L+RVSDLLRW+ K+P F L WKLK+FP+FS+SSPLYHT  +P PLT +EE+ L+ +H R+
Sbjct: 206 LKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPEPLTAEEERELEAAHGRI 265

Query: 264 MKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD-NNPIVYGTVQAYLKDAKD 323
            +IC+ C ++NVPL IDAE T +QPAIDY  YS+AI+ N D + PIVY T+QAYL+DA +
Sbjct: 266 QEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKDRPIVYNTIQAYLRDAGE 325

Query: 324 RLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGFESPIHNSIQETHSCYNSCASFL 383
           RL L  + A+   VP+G KLVRGAYMSSE+ +A SLG +SP+H++IQ+THSCYN C +FL
Sbjct: 326 RLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVHDTIQDTHSCYNDCMTFL 385

Query: 384 LDNIANGSS-GVILATHNVESGKLAASKAYDIGIGKLKQKLEFAQLYGMSEALSFGLRNA 443
           ++  +NGS  GV+LATHN +SG+LA+ KA D+GI K   K+EFAQLYGMS+ALSFGL+ A
Sbjct: 386 MEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEFAQLYGMSDALSFGLKRA 445

Query: 444 GFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQLMRKEMARRV 487
           GF VSKYMPFGPV   +PYLLRRA ENRG+++T   DRQLMR E+ RR+
Sbjct: 446 GFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMRMELKRRL 494

BLAST of MC04g1779 vs. ExPASy Swiss-Prot
Match: Q6NKX1 (Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 PE=2 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 2.8e-141
Identity = 248/438 (56.62%), Postives = 326/438 (74.43%), Query Frame = 0

Query: 52  PSATPNIDFADTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDA 111
           P    ++D +D   LF SV    LLR+ A LH  ++GP+VD+G W+M+S+LM+  V RD 
Sbjct: 36  PKPEVDLDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMSSKLMDTTVTRDL 95

Query: 112 VLGAVRHSFYQNFCAGEDYAAVGNTVRRLNDA-GLRSMLDYALEYADDDVACDQNLDGFL 171
           VL  V+ +FY +FCAGED AA    V  + ++ GL+ ML Y +E+A+D  ACD+N+  F+
Sbjct: 96  VLRIVKGTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAEDGGACDENIQKFI 155

Query: 172 RTIESTRSLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQ 231
            T+E+ ++LP +  S VV K++AICP+ +L+RVSDLLRWQ KNP F LPWKL +FP+FS 
Sbjct: 156 ETVEAAKTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSG 215

Query: 232 SSPLYHTLHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFT 291
            SPLYHT  +P PLT +EE+ L+ +H+RL  +C  C ++NVPL IDAE T +QPAIDY  
Sbjct: 216 LSPLYHTTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMA 275

Query: 292 YSAAIIHNKD-NNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESK 351
           Y +AI+ N D + PIVY T+QAYLKDA +RL L  + ++ M VP+G KLVRGAYMSSE+K
Sbjct: 276 YWSAIMFNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAK 335

Query: 352 IASSLGFESPIHNSIQETHSCYNSCASFLLDNIANGSS-GVILATHNVESGKLAASKAYD 411
           +A SLG++SP+H++IQ TH CYN C SFL++  +NGS   VILATHN +SGKL A KA +
Sbjct: 336 LADSLGYKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASE 395

Query: 412 IGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLL 471
           +GI K   K+EFAQLYGMS+ALSFGL+ AGF VSKYMP+GPVD  +PYL+RRA ENRG++
Sbjct: 396 LGINKENGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMM 455

Query: 472 STSNLDRQLMRKEMARRV 487
           ST  LDRQLMRKE+ RRV
Sbjct: 456 STGALDRQLMRKELKRRV 473

BLAST of MC04g1779 vs. ExPASy Swiss-Prot
Match: Q86H28 (Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=prodh PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.4e-47
Identity = 144/441 (32.65%), Postives = 225/441 (51.02%), Query Frame = 0

Query: 62  DTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGAVRHSFY 121
           DT  L+ S ST  L      L V ++  + D     +N  L E   +   +   +++SF+
Sbjct: 145 DTSKLYVSKSTGELFFTFMILKVCSINFISDNSQKFLN--LFEKLGLSKPLNFFIKYSFF 204

Query: 122 QNFCAGEDYAAVGNTVRRLNDAGLRSMLDYAL-EYADDDVACDQNLDGFLRTIESTRSLP 181
           + FCAGE          +LN  G+ ++LDYA+ E A      D   +    TI      P
Sbjct: 205 KQFCAGETIRETEIFTEKLNKLGIGTILDYAIEELAGSSEGFDSVAENICETIRVAAKNP 264

Query: 182 PASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHTLHQ 241
             + SF   K + +    +LE+++ L+         N+   +   PI S +SPL   L+Q
Sbjct: 265 --TNSFSCVKFTGLVTPSVLEKMNTLVS--------NVTTNVSELPIESFNSPLDFYLNQ 324

Query: 242 PNPLTPQEEQSLQIS---------HQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTY 301
            + L  Q  + L  S           R+ KI Q C    VP+ +DAE +  Q AI + T 
Sbjct: 325 SSSLMKQGSEPLLTSKDIKEIKEFFNRMDKIFQLCHQRGVPILVDAEQSYYQVAIHHLTM 384

Query: 302 SAAIIHNKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMK--VPLGIKLVRGAYMSSESK 361
           S +I +NK+  PI+Y T Q YL +  + L    + +   K    LG K+VRGAYM +ES+
Sbjct: 385 SYSIKYNKE-KPIIYNTYQMYLVNGMNVLKQHFELSSSQKFNFKLGAKIVRGAYMVTESE 444

Query: 362 IASSLGFESPIHNSIQETHSCYNSCASFLLDNIAN--GSSGVILATHNVESGKLAASKAY 421
            +  L  E+P+  +IQ+TH  YN+   FLL+ I +   S G+++A+HN +S  L      
Sbjct: 445 RSQRLSTENPVLPTIQDTHKSYNTALDFLLNQIKSDPNSIGLMIASHNEDSINLGTKLIK 504

Query: 422 DIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGL 481
              I      ++F QL+GM++ LSF L +   ++ KY+PFGPV+ V+PYL+RR  EN+G 
Sbjct: 505 QYKIDPTNPNIQFGQLFGMADFLSFNLVDQHQRIFKYVPFGPVEEVLPYLIRRMHENKGF 564

Query: 482 LSTSNLDRQL--MRKEMARRV 487
           +  SN D++L  ++KE+ RR+
Sbjct: 565 IG-SNSDKELFYLKKEIKRRL 571

BLAST of MC04g1779 vs. ExPASy Swiss-Prot
Match: Q9UF12 (Hydroxyproline dehydrogenase OS=Homo sapiens OX=9606 GN=PRODH2 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 5.3e-39
Identity = 133/430 (30.93%), Postives = 213/430 (49.53%), Query Frame = 0

Query: 72  TPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGA-VRHSFYQNFCAGEDY 131
           T  L RA   L + A  P+V  G+ +   +     ++   + GA +R S Y  F AGE  
Sbjct: 110 TGELTRALLVLRLCAWPPLVTHGLLL---QAWSRRLLGSRLSGAFLRASVYGQFVAGETA 169

Query: 132 AAVGNTVRRLNDAGLRSMLDYALEYADDDVA------CDQNLDGFLRTIESTRSL--PP- 191
             V   V++L    LR +L    E   D  A       + NL   LR ++ +R L  PP 
Sbjct: 170 EEVKGCVQQLRTLSLRPLLAVPTEEEPDSAAKSGEAWYEGNLGAMLRCVDLSRGLLEPPS 229

Query: 192 -ASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHTLHQ 251
            A AS +  KV+A+   RL + ++  +R     P  +L    +       S        Q
Sbjct: 230 LAEASLMQLKVTALTSTRLCKELASWVR----RPGASLELSPERLAEAMDSGQNL----Q 289

Query: 252 PNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK- 311
            + L  ++ Q L+ S  RL ++ Q     +V L +DAE+T + PA+     + A+  N  
Sbjct: 290 VSCLNAEQNQHLRASLSRLHRVAQYARAQHVRLLVDAEYTSLNPALSLLVAALAVRWNSP 349

Query: 312 -DNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGFES 371
            +  P V+ T QA LKD  +RL   A+AA    +  G+KLVRGAY+  E  +A   G E 
Sbjct: 350 GEGGPWVWNTYQACLKDTFERLGRDAEAAHRAGLAFGVKLVRGAYLDKERAVAQLHGMED 409

Query: 372 PIHNSIQETHSCYNSCASFLLDNIANGS--SGVILATHNVESGKLAASKAYDIGIGKLKQ 431
           P     + T   Y+ C   +L ++A       +++A+HN ES + A  + +++GI  L  
Sbjct: 410 PTQPDYEATSQSYSRCLELMLTHVARHGPMCHLMVASHNEESVRQATKRMWELGI-PLDG 469

Query: 432 KLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQ 487
            + F QL GM + +S  L  AG+ V K +P+G ++ V+PYL+RRA+ENR +L  +  +++
Sbjct: 470 TVCFGQLLGMCDHVSLALGQAGYVVYKSIPYGSLEEVIPYLIRRAQENRSVLQGARREQE 527

BLAST of MC04g1779 vs. ExPASy Swiss-Prot
Match: Q8VCZ9 (Hydroxyproline dehydrogenase OS=Mus musculus OX=10090 GN=Prodh2 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.6e-38
Identity = 132/430 (30.70%), Postives = 215/430 (50.00%), Query Frame = 0

Query: 72  TPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGA-VRHSFYQNFCAGEDY 131
           T  L RA   L + A  P+V  G   +  +     ++   + GA +R S Y  F AGE  
Sbjct: 34  TAELARALLVLRLCAWPPLVTHG---LAFQAWSQRLLGSRLSGALLRASIYGQFVAGETA 93

Query: 132 AAVGNTVRRLNDAGLRSMLDYALEYADDDVA------CDQNLDGFLRTIESTRSL----P 191
             V N V +L   GL+ +L    E   D  A       ++NL   LR ++ +R+L     
Sbjct: 94  EEVRNCVGQLQALGLQPLLAVPTEEEPDSTAKTSEVWYEENLSAMLRCVDLSRALVDAHG 153

Query: 192 PASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHTLHQ 251
           PA  S +  KV+A+   RL + +S  ++ + +      P +L      +++      L Q
Sbjct: 154 PARNSLMQLKVTALASTRLCKELSAWIQ-RPRGSSELSPERL------AEAMDSGRNL-Q 213

Query: 252 PNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHN-- 311
            + L+ ++ Q LQ S  RL ++ Q      V L +DAE+T + PA+     + A+  N  
Sbjct: 214 LSCLSTEQNQHLQASLSRLHRVAQHARAKCVRLLVDAEYTFINPALSLLVAALAVRWNSP 273

Query: 312 KDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGFES 371
           ++  P V+ T QAYLKD   RL   A+AA    +  G+KLVRGAY+  E  +    G E 
Sbjct: 274 EEGGPWVWNTYQAYLKDTHQRLEQDAEAAHKAGLAFGVKLVRGAYLDKERSMTQLQGKED 333

Query: 372 PIHNSIQETHSCYNSCASFLLDNIANGS--SGVILATHNVESGKLAASKAYDIGIGKLKQ 431
                 + T   Y+ C   +L  ++N      +++A+HN ES + A  + +++GI  L  
Sbjct: 334 CTQPDYEATSRSYSRCLELMLRCVSNHGPPCHLMVASHNEESVRQATKRMWELGI-PLDG 393

Query: 432 KLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQ 487
            + F QL GM + +S  L  AG+ V K +P+G ++ V+PYL+RRA+ENR +L  +  ++ 
Sbjct: 394 PVCFGQLLGMCDHVSLALGQAGYMVYKSIPYGCLEEVIPYLIRRAQENRSVLQGARREQA 451

BLAST of MC04g1779 vs. NCBI nr
Match: XP_022133477.1 (proline dehydrogenase 2, mitochondrial-like [Momordica charantia])

HSP 1 Score: 787 bits (2032), Expect = 2.07e-285
Identity = 397/397 (100.00%), Postives = 397/397 (100.00%), Query Frame = 0

Query: 95  MWVMNSRLMEVEVIRDAVLGAVRHSFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALE 154
           MWVMNSRLMEVEVIRDAVLGAVRHSFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALE
Sbjct: 1   MWVMNSRLMEVEVIRDAVLGAVRHSFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALE 60

Query: 155 YADDDVACDQNLDGFLRTIESTRSLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNP 214
           YADDDVACDQNLDGFLRTIESTRSLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNP
Sbjct: 61  YADDDVACDQNLDGFLRTIESTRSLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNP 120

Query: 215 CFNLPWKLKTFPIFSQSSPLYHTLHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLA 274
           CFNLPWKLKTFPIFSQSSPLYHTLHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLA
Sbjct: 121 CFNLPWKLKTFPIFSQSSPLYHTLHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLA 180

Query: 275 IDAEHTKVQPAIDYFTYSAAIIHNKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPL 334
           IDAEHTKVQPAIDYFTYSAAIIHNKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPL
Sbjct: 181 IDAEHTKVQPAIDYFTYSAAIIHNKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPL 240

Query: 335 GIKLVRGAYMSSESKIASSLGFESPIHNSIQETHSCYNSCASFLLDNIANGSSGVILATH 394
           GIKLVRGAYMSSESKIASSLGFESPIHNSIQETHSCYNSCASFLLDNIANGSSGVILATH
Sbjct: 241 GIKLVRGAYMSSESKIASSLGFESPIHNSIQETHSCYNSCASFLLDNIANGSSGVILATH 300

Query: 395 NVESGKLAASKAYDIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVM 454
           NVESGKLAASKAYDIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVM
Sbjct: 301 NVESGKLAASKAYDIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVM 360

Query: 455 PYLLRRAEENRGLLSTSNLDRQLMRKEMARRVKEYLG 491
           PYLLRRAEENRGLLSTSNLDRQLMRKEMARRVKEYLG
Sbjct: 361 PYLLRRAEENRGLLSTSNLDRQLMRKEMARRVKEYLG 397

BLAST of MC04g1779 vs. NCBI nr
Match: XP_023524135.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 764 bits (1974), Expect = 4.30e-275
Identity = 394/488 (80.74%), Postives = 438/488 (89.75%), Query Frame = 0

Query: 1   MARRVNRHQAKILKNLSFLAFTRPLNSSAAASSPAAAAFSAADSPLHLTTTPSATPNIDF 60
           MARR+   Q+K  K      F R LN+  AASSPAAA  +AA SPL+LT +P A P +DF
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNT--AASSPAAAFTAAAASPLNLTGSP-AKPTVDF 60

Query: 61  ADTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGAVRHSF 120
            DT ALFGS+ST  LLRAAANLH+AAVGPVVD G+W+MNS+LM+VE+IR+A+LG V+H+F
Sbjct: 61  TDTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIREAILGTVKHTF 120

Query: 121 YQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALEYADDDVACDQNLDGFLRTIESTRSLP 180
           Y++FCAGED AAV  TVRRL+D GLRSMLDYALEYADD+ +CD+NLDGFL+TIE+T+SLP
Sbjct: 121 YRHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLP 180

Query: 181 PASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHTLHQ 240
           P SASFVVAKVSAICPLRLLERVSDLLRWQ KNPC NLPWKLKT PIFS SSPLYHTL  
Sbjct: 181 PGSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKH 240

Query: 241 PNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 300
           P PLTPQEE+SLQISHQRL+KICQ+C DANVPLAIDAEHTKVQPAIDYFTYSAAI +NKD
Sbjct: 241 PTPLTPQEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKD 300

Query: 301 NNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGFESPI 360
           +NPIVYGTVQAYLKDAKDRLLLVAKAAD+MK+PLGIKLVRGAYMSSESK+ASSLGF SPI
Sbjct: 301 HNPIVYGTVQAYLKDAKDRLLLVAKAADNMKIPLGIKLVRGAYMSSESKLASSLGFSSPI 360

Query: 361 HNSIQETHSCYNSCASFLLDNIANGSSGVILATHNVESGKLAASKAYDIGIGKLKQKLEF 420
           H++I +THSCY+ CASFLLDN+   SSGVILATHNVESGKLAA+KAY+IGIGKLK KLEF
Sbjct: 361 HDTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAAAKAYEIGIGKLKHKLEF 420

Query: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQLMRK 480
           AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDR+LMRK
Sbjct: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLMRK 480

Query: 481 EMARRVKE 488
           EMARRVK+
Sbjct: 481 EMARRVKD 485

BLAST of MC04g1779 vs. NCBI nr
Match: XP_023551146.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 765 bits (1975), Expect = 4.39e-275
Identity = 396/494 (80.16%), Postives = 443/494 (89.68%), Query Frame = 0

Query: 1   MARRVNRHQAKILKNLSFLAFTRPLNSSAAASSPAAAAFSAADSP----LHLTTTPSATP 60
           MAR +   Q+KI KN+ F AF R LN+  AASS AAA  SAA +P    L+L  +P+A P
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNT--AASSSAAAFTSAASTPPPPPLNLAVSPAA-P 60

Query: 61  NIDFADTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGAV 120
            +DF+DT+ALFGS+ST  +L AAA+LHVAAVGPVVDVGMWVMNS+LM+VE+ RD + G V
Sbjct: 61  TVDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTV 120

Query: 121 RHSFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALEYADDDVACDQNLDGFLRTIEST 180
           RHSFY++FCAGED  AVG TVRRL+D GLRSMLDYA+EYA+D+ +CD+NL GFLRTIE+T
Sbjct: 121 RHSFYRHFCAGEDDTAVGKTVRRLHDVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEAT 180

Query: 181 RSLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYH 240
           R+LPP SASFVVAKVSAICPLRLLERVSDLLRWQQK+PC NLPWKLKTFPIFS+SSPLYH
Sbjct: 181 RALPPGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYH 240

Query: 241 TLHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAII 300
           TL QPNPLT +E++SLQISHQRLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAII
Sbjct: 241 TLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAII 300

Query: 301 HNKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGF 360
           HNKD+NPIVYGT+QAYLKDAKDRLLLV K A+ M+VPLGIKLVRGAYMSSESK+ASSLGF
Sbjct: 301 HNKDHNPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGF 360

Query: 361 ESPIHNSIQETHSCYNSCASFLLDNIANGSSGVILATHNVESGKLAASKAYDIGIGKLKQ 420
           ESPIHNSI +TH+CYNSCASFLLDNIA GSSG ILATHNV+SGKLAA KAY+IGI KLK+
Sbjct: 361 ESPIHNSIHDTHACYNSCASFLLDNIAVGSSGAILATHNVDSGKLAAKKAYEIGIEKLKE 420

Query: 421 KLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQ 480
           KLEFAQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLS SNLDRQ
Sbjct: 421 KLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQ 480

Query: 481 LMRKEMARRVKEYL 490
           LMRKE+ RR+KEYL
Sbjct: 481 LMRKELRRRMKEYL 491

BLAST of MC04g1779 vs. NCBI nr
Match: XP_022939392.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 763 bits (1971), Expect = 1.72e-274
Identity = 396/493 (80.32%), Postives = 442/493 (89.66%), Query Frame = 0

Query: 1   MARRVNRHQAKILKNLSFLAFTRPLNSSAAASSPAAAAFSAADSP---LHLTTTPSATPN 60
           MAR +   Q+KI KN+ F AF R LN+  AASS AAA  SAA +P   L+L  +P+A P 
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNT--AASSSAAAFTSAASTPPPHLNLAVSPAA-PT 60

Query: 61  IDFADTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGAVR 120
           +DF+DT+ALFGS+ST  +L AAA+LHVAAVGPVVDVGMWVMNS+LM+VE+ RD + G VR
Sbjct: 61  VDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVR 120

Query: 121 HSFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALEYADDDVACDQNLDGFLRTIESTR 180
           HSFY++FCAGED  AVG TVRRL++ GLRSMLDYA+EYA+D+ +CD+NL GFLRTIE+TR
Sbjct: 121 HSFYRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATR 180

Query: 181 SLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHT 240
            LP  SASFVVAKVSAICPLRLLERVSDLLRWQQK+PC NLPWKLKTFPIFS+SSPLYHT
Sbjct: 181 VLPRGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHT 240

Query: 241 LHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIH 300
           L QPNPLT +E++SLQISHQRLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIH
Sbjct: 241 LQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIH 300

Query: 301 NKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGFE 360
           NKD+NPIVYGT+QAYLKDAKDRLLLV K A++M+VPLGIKLVRGAYMSSESK+ASSLGFE
Sbjct: 301 NKDHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFE 360

Query: 361 SPIHNSIQETHSCYNSCASFLLDNIANGSSGVILATHNVESGKLAASKAYDIGIGKLKQK 420
           SPIHNSI +TH+CYNSCASFLLDNIA GSSG ILATHNVESGKLAA KAY+IGI KLK+K
Sbjct: 361 SPIHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKK 420

Query: 421 LEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQL 480
           LEFAQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLS SNLDRQL
Sbjct: 421 LEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQL 480

Query: 481 MRKEMARRVKEYL 490
           MRKE+ RRVKEYL
Sbjct: 481 MRKELGRRVKEYL 490

BLAST of MC04g1779 vs. NCBI nr
Match: XP_022923652.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 762 bits (1968), Expect = 3.79e-274
Identity = 396/490 (80.82%), Postives = 440/490 (89.80%), Query Frame = 0

Query: 1   MARRVNRHQAKILKNLSFLAFTRPLNSSAAASSPAAA--AFSAADSPLHLTTTPSATPNI 60
           MARR+   Q+K  K      F R LN+  AASSPAAA  A +A+ SPL+ T +P ATP I
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNT--AASSPAAAFTAAAASASPLNHTGSP-ATPTI 60

Query: 61  DFADTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGAVRH 120
           DF DT ALFGS+ST  LLRAAANLH+AAVGPVVD G+W+MNS+LM+VE+IRDA+LG+V+H
Sbjct: 61  DFTDTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKH 120

Query: 121 SFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALEYADDDVACDQNLDGFLRTIESTRS 180
           +FY++FCAGED AAV  TVRRL+D GLRSMLDYALEYADD+ +CD+NLDGFL+TIE+T+S
Sbjct: 121 TFYRHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKS 180

Query: 181 LPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHTL 240
           LPP SASFVVAKVSAICPLRLLERVSDLLRWQ KNPC NLPWKLKT PIFS SSPLYHTL
Sbjct: 181 LPPGSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTL 240

Query: 241 HQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHN 300
             P PLTPQEE+SLQISHQRL+KICQ+C DANVPLAIDAEHTKVQPAIDYFTYSAAI +N
Sbjct: 241 KHPTPLTPQEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYN 300

Query: 301 KDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGFES 360
           KD+NPIVYGTVQAYLKDAKDRLLLVAKAAD+MK+PLGIKLVRGAYMSSESK+ASSLGF S
Sbjct: 301 KDHNPIVYGTVQAYLKDAKDRLLLVAKAADNMKIPLGIKLVRGAYMSSESKLASSLGFPS 360

Query: 361 PIHNSIQETHSCYNSCASFLLDNIANGSSGVILATHNVESGKLAASKAYDIGIGKLKQKL 420
           PIH++I +THSCY+ CASFLLDN+   SSGVILATHNVESGKLAA+KAY+IGIGKLK KL
Sbjct: 361 PIHDTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKL 420

Query: 421 EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQLM 480
           EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDR+LM
Sbjct: 421 EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 480

Query: 481 RKEMARRVKE 488
           RKEMARRVK+
Sbjct: 481 RKEMARRVKD 487

BLAST of MC04g1779 vs. ExPASy TrEMBL
Match: A0A6J1BWT6 (Proline dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111006047 PE=3 SV=1)

HSP 1 Score: 787 bits (2032), Expect = 1.00e-285
Identity = 397/397 (100.00%), Postives = 397/397 (100.00%), Query Frame = 0

Query: 95  MWVMNSRLMEVEVIRDAVLGAVRHSFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALE 154
           MWVMNSRLMEVEVIRDAVLGAVRHSFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALE
Sbjct: 1   MWVMNSRLMEVEVIRDAVLGAVRHSFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALE 60

Query: 155 YADDDVACDQNLDGFLRTIESTRSLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNP 214
           YADDDVACDQNLDGFLRTIESTRSLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNP
Sbjct: 61  YADDDVACDQNLDGFLRTIESTRSLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNP 120

Query: 215 CFNLPWKLKTFPIFSQSSPLYHTLHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLA 274
           CFNLPWKLKTFPIFSQSSPLYHTLHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLA
Sbjct: 121 CFNLPWKLKTFPIFSQSSPLYHTLHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLA 180

Query: 275 IDAEHTKVQPAIDYFTYSAAIIHNKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPL 334
           IDAEHTKVQPAIDYFTYSAAIIHNKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPL
Sbjct: 181 IDAEHTKVQPAIDYFTYSAAIIHNKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPL 240

Query: 335 GIKLVRGAYMSSESKIASSLGFESPIHNSIQETHSCYNSCASFLLDNIANGSSGVILATH 394
           GIKLVRGAYMSSESKIASSLGFESPIHNSIQETHSCYNSCASFLLDNIANGSSGVILATH
Sbjct: 241 GIKLVRGAYMSSESKIASSLGFESPIHNSIQETHSCYNSCASFLLDNIANGSSGVILATH 300

Query: 395 NVESGKLAASKAYDIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVM 454
           NVESGKLAASKAYDIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVM
Sbjct: 301 NVESGKLAASKAYDIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVM 360

Query: 455 PYLLRRAEENRGLLSTSNLDRQLMRKEMARRVKEYLG 491
           PYLLRRAEENRGLLSTSNLDRQLMRKEMARRVKEYLG
Sbjct: 361 PYLLRRAEENRGLLSTSNLDRQLMRKEMARRVKEYLG 397

BLAST of MC04g1779 vs. ExPASy TrEMBL
Match: A0A6J1FGN7 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1)

HSP 1 Score: 763 bits (1971), Expect = 8.31e-275
Identity = 396/493 (80.32%), Postives = 442/493 (89.66%), Query Frame = 0

Query: 1   MARRVNRHQAKILKNLSFLAFTRPLNSSAAASSPAAAAFSAADSP---LHLTTTPSATPN 60
           MAR +   Q+KI KN+ F AF R LN+  AASS AAA  SAA +P   L+L  +P+A P 
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNT--AASSSAAAFTSAASTPPPHLNLAVSPAA-PT 60

Query: 61  IDFADTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGAVR 120
           +DF+DT+ALFGS+ST  +L AAA+LHVAAVGPVVDVGMWVMNS+LM+VE+ RD + G VR
Sbjct: 61  VDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVR 120

Query: 121 HSFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALEYADDDVACDQNLDGFLRTIESTR 180
           HSFY++FCAGED  AVG TVRRL++ GLRSMLDYA+EYA+D+ +CD+NL GFLRTIE+TR
Sbjct: 121 HSFYRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATR 180

Query: 181 SLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHT 240
            LP  SASFVVAKVSAICPLRLLERVSDLLRWQQK+PC NLPWKLKTFPIFS+SSPLYHT
Sbjct: 181 VLPRGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHT 240

Query: 241 LHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIH 300
           L QPNPLT +E++SLQISHQRLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIH
Sbjct: 241 LQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIH 300

Query: 301 NKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGFE 360
           NKD+NPIVYGT+QAYLKDAKDRLLLV K A++M+VPLGIKLVRGAYMSSESK+ASSLGFE
Sbjct: 301 NKDHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFE 360

Query: 361 SPIHNSIQETHSCYNSCASFLLDNIANGSSGVILATHNVESGKLAASKAYDIGIGKLKQK 420
           SPIHNSI +TH+CYNSCASFLLDNIA GSSG ILATHNVESGKLAA KAY+IGI KLK+K
Sbjct: 361 SPIHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKK 420

Query: 421 LEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQL 480
           LEFAQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLS SNLDRQL
Sbjct: 421 LEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQL 480

Query: 481 MRKEMARRVKEYL 490
           MRKE+ RRVKEYL
Sbjct: 481 MRKELGRRVKEYL 490

BLAST of MC04g1779 vs. ExPASy TrEMBL
Match: A0A6J1E6Q2 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111431296 PE=3 SV=1)

HSP 1 Score: 762 bits (1968), Expect = 1.83e-274
Identity = 396/490 (80.82%), Postives = 440/490 (89.80%), Query Frame = 0

Query: 1   MARRVNRHQAKILKNLSFLAFTRPLNSSAAASSPAAA--AFSAADSPLHLTTTPSATPNI 60
           MARR+   Q+K  K      F R LN+  AASSPAAA  A +A+ SPL+ T +P ATP I
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNT--AASSPAAAFTAAAASASPLNHTGSP-ATPTI 60

Query: 61  DFADTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGAVRH 120
           DF DT ALFGS+ST  LLRAAANLH+AAVGPVVD G+W+MNS+LM+VE+IRDA+LG+V+H
Sbjct: 61  DFTDTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKH 120

Query: 121 SFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALEYADDDVACDQNLDGFLRTIESTRS 180
           +FY++FCAGED AAV  TVRRL+D GLRSMLDYALEYADD+ +CD+NLDGFL+TIE+T+S
Sbjct: 121 TFYRHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKS 180

Query: 181 LPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHTL 240
           LPP SASFVVAKVSAICPLRLLERVSDLLRWQ KNPC NLPWKLKT PIFS SSPLYHTL
Sbjct: 181 LPPGSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTL 240

Query: 241 HQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHN 300
             P PLTPQEE+SLQISHQRL+KICQ+C DANVPLAIDAEHTKVQPAIDYFTYSAAI +N
Sbjct: 241 KHPTPLTPQEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYN 300

Query: 301 KDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGFES 360
           KD+NPIVYGTVQAYLKDAKDRLLLVAKAAD+MK+PLGIKLVRGAYMSSESK+ASSLGF S
Sbjct: 301 KDHNPIVYGTVQAYLKDAKDRLLLVAKAADNMKIPLGIKLVRGAYMSSESKLASSLGFPS 360

Query: 361 PIHNSIQETHSCYNSCASFLLDNIANGSSGVILATHNVESGKLAASKAYDIGIGKLKQKL 420
           PIH++I +THSCY+ CASFLLDN+   SSGVILATHNVESGKLAA+KAY+IGIGKLK KL
Sbjct: 361 PIHDTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKL 420

Query: 421 EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQLM 480
           EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDR+LM
Sbjct: 421 EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 480

Query: 481 RKEMARRVKE 488
           RKEMARRVK+
Sbjct: 481 RKEMARRVKD 487

BLAST of MC04g1779 vs. ExPASy TrEMBL
Match: A0A5D3BNA9 (Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002050 PE=3 SV=1)

HSP 1 Score: 761 bits (1965), Expect = 4.53e-274
Identity = 388/491 (79.02%), Postives = 432/491 (87.98%), Query Frame = 0

Query: 1   MARRVNRHQAKILKNLSFLAFTRPLNSSAAASSPAAAAFSAADSPLHLTTTPSATPNIDF 60
           MAR +   Q+KI K +    F RPLN+  AASSPAAA F+A   PLHL   P +TP +DF
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNT--AASSPAAA-FTAPPPPLHLN--PPSTPTVDF 60

Query: 61  ADTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGAVRHSF 120
            D+RALFGS+ T  LL A A LH +A+GPVVDVGMWVMNS+LM+VE+ RD VLG V+H+F
Sbjct: 61  TDSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHTF 120

Query: 121 YQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALEYADDDVACDQNLDGFLRTIESTRSLP 180
           Y++FCAGED  +V  TVRRL+D GLRSMLDYALEYADD+ +CD+NLDGFLRT+E+T+SLP
Sbjct: 121 YRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSLP 180

Query: 181 PASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHTLHQ 240
             SASFVV KVSAICPLRLLERVSDLLRWQQKNP FNLPWKL+T PIFS+SSPLYHTL Q
Sbjct: 181 SGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQ 240

Query: 241 PNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 300
           P PLT +EE+SLQISH+RLMKICQSCVD NVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD
Sbjct: 241 PEPLTREEEKSLQISHERLMKICQSCVDTNVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 300

Query: 301 NNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGFESPI 360
            NPIVYGT+QAYLKDAKDRLLL  K A  +K+PLGIKLVRGAYMSSESK+ASSLGFESPI
Sbjct: 301 RNPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIQETHSCYNSCASFLLDNIANGSSGVILATHNVESGKLAASKAYDIGIGKLKQKLEF 420
           HN+IQ+THSCYN+CASFLLD+IA GSSG ILATHNVESGKLAA++AY+IGIGKLKQKLEF
Sbjct: 361 HNTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEF 420

Query: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQLMRK 480
           AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLS SNLDRQLMRK
Sbjct: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 EMARRVKEYLG 491
           E+ RR+KEYL 
Sbjct: 481 ELGRRMKEYLS 486

BLAST of MC04g1779 vs. ExPASy TrEMBL
Match: A0A0A0KXN4 (Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1)

HSP 1 Score: 757 bits (1955), Expect = 1.88e-272
Identity = 390/497 (78.47%), Postives = 435/497 (87.53%), Query Frame = 0

Query: 1   MARRVNRHQAKILKNLSFLAFTRPLNSSAAASSPAAAAFSAAD------SPLHLTTTPSA 60
           MA+ +   Q+KI K + F  F RPLN+  AASSPAAA F+AA       SPLHL   P +
Sbjct: 1   MAQAIVSTQSKISKRVFFYVFNRPLNT--AASSPAAA-FTAATATTSPPSPLHLN--PPS 60

Query: 61  TPNIDFADTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLG 120
           TP +DF D+RALFGS+ T  LL A A LH AAVGPVVDVGMWVMNS+LM+VE+ RD VLG
Sbjct: 61  TPTVDFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLG 120

Query: 121 AVRHSFYQNFCAGEDYAAVGNTVRRLNDAGLRSMLDYALEYADDDVACDQNLDGFLRTIE 180
            V+HSFY++FCAGED  +V  TVRRL+D GLRSMLDYALEYADD+ +CD+NLDGFL T+E
Sbjct: 121 TVKHSFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVE 180

Query: 181 STRSLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPL 240
           +T+SLP  SASFVV KVSAICPLRLLERVSDLLRWQQKNP FNLPWKL+T PIFS+SSPL
Sbjct: 181 ATKSLPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPL 240

Query: 241 YHTLHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAA 300
           YHTL QP PLT +EE+SLQ+SH+RLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAA
Sbjct: 241 YHTLEQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAA 300

Query: 301 IIHNKDNNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSL 360
           IIHNKD NPIVYGT+QAYLKDAKDRLLL  K A  +KVPLGIKLVRGAYMSSESK+ASSL
Sbjct: 301 IIHNKDRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSL 360

Query: 361 GFESPIHNSIQETHSCYNSCASFLLDNIANGSSGVILATHNVESGKLAASKAYDIGIGKL 420
           GFESPIH++IQ+THSCYN+CASFLLD+IA GS+G ILATHNVESGKLAAS+AY+IGIGKL
Sbjct: 361 GFESPIHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKL 420

Query: 421 KQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLD 480
           KQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLS SNLD
Sbjct: 421 KQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLD 480

Query: 481 RQLMRKEMARRVKEYLG 491
           R+LMRKE+ RR+KEY+ 
Sbjct: 481 RELMRKELGRRMKEYIS 492

BLAST of MC04g1779 vs. TAIR 10
Match: AT3G30775.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 505.0 bits (1299), Expect = 6.9e-143
Identity = 260/469 (55.44%), Postives = 344/469 (73.35%), Query Frame = 0

Query: 24  PLNSSAAASSPAAAAF--SAADSPL-HLTTTPSATPNIDFADTRALFGSVSTPALLRAAA 83
           P  +++ A  P   +F   A + PL H   T  +   +D +D   LF S+ T  LLR+ A
Sbjct: 26  PTVTASTAVVPEILSFGQQAPEPPLHHPKPTEQSHDGLDLSDQARLFSSIPTSDLLRSTA 85

Query: 84  NLHVAAVGPVVDVGMWVMNSRLMEVEVIRDAVLGAVRHSFYQNFCAGEDYAAVGNTVRRL 143
            LH AA+GP+VD+G WVM+S+LM+  V R  VLG V+ +FY +FCAGED  A    VR +
Sbjct: 86  VLHAAAIGPMVDLGTWVMSSKLMDASVTRGMVLGLVKSTFYDHFCAGEDADAAAERVRSV 145

Query: 144 NDA-GLRSMLDYALEYADDDVACDQNLDGFLRTIESTRSLPPASASFVVAKVSAICPLRL 203
            +A GL+ ML Y +E+ADD V+CD N+  F+RTIE+ +SLP +  S VV K++AICP+ L
Sbjct: 146 YEATGLKGMLVYGVEHADDAVSCDDNMQQFIRTIEAAKSLPTSHFSSVVVKITAICPISL 205

Query: 204 LERVSDLLRWQQKNPCFNLPWKLKTFPIFSQSSPLYHTLHQPNPLTPQEEQSLQISHQRL 263
           L+RVSDLLRW+ K+P F L WKLK+FP+FS+SSPLYHT  +P PLT +EE+ L+ +H R+
Sbjct: 206 LKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPEPLTAEEERELEAAHGRI 265

Query: 264 MKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD-NNPIVYGTVQAYLKDAKD 323
            +IC+ C ++NVPL IDAE T +QPAIDY  YS+AI+ N D + PIVY T+QAYL+DA +
Sbjct: 266 QEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKDRPIVYNTIQAYLRDAGE 325

Query: 324 RLLLVAKAADDMKVPLGIKLVRGAYMSSESKIASSLGFESPIHNSIQETHSCYNSCASFL 383
           RL L  + A+   VP+G KLVRGAYMSSE+ +A SLG +SP+H++IQ+THSCYN C +FL
Sbjct: 326 RLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVHDTIQDTHSCYNDCMTFL 385

Query: 384 LDNIANGSS-GVILATHNVESGKLAASKAYDIGIGKLKQKLEFAQLYGMSEALSFGLRNA 443
           ++  +NGS  GV+LATHN +SG+LA+ KA D+GI K   K+EFAQLYGMS+ALSFGL+ A
Sbjct: 386 MEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEFAQLYGMSDALSFGLKRA 445

Query: 444 GFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQLMRKEMARRV 487
           GF VSKYMPFGPV   +PYLLRRA ENRG+++T   DRQLMR E+ RR+
Sbjct: 446 GFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMRMELKRRL 494

BLAST of MC04g1779 vs. TAIR 10
Match: AT5G38710.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 503.4 bits (1295), Expect = 2.0e-142
Identity = 248/438 (56.62%), Postives = 326/438 (74.43%), Query Frame = 0

Query: 52  PSATPNIDFADTRALFGSVSTPALLRAAANLHVAAVGPVVDVGMWVMNSRLMEVEVIRDA 111
           P    ++D +D   LF SV    LLR+ A LH  ++GP+VD+G W+M+S+LM+  V RD 
Sbjct: 36  PKPEVDLDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMSSKLMDTTVTRDL 95

Query: 112 VLGAVRHSFYQNFCAGEDYAAVGNTVRRLNDA-GLRSMLDYALEYADDDVACDQNLDGFL 171
           VL  V+ +FY +FCAGED AA    V  + ++ GL+ ML Y +E+A+D  ACD+N+  F+
Sbjct: 96  VLRIVKGTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAEDGGACDENIQKFI 155

Query: 172 RTIESTRSLPPASASFVVAKVSAICPLRLLERVSDLLRWQQKNPCFNLPWKLKTFPIFSQ 231
            T+E+ ++LP +  S VV K++AICP+ +L+RVSDLLRWQ KNP F LPWKL +FP+FS 
Sbjct: 156 ETVEAAKTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSG 215

Query: 232 SSPLYHTLHQPNPLTPQEEQSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFT 291
            SPLYHT  +P PLT +EE+ L+ +H+RL  +C  C ++NVPL IDAE T +QPAIDY  
Sbjct: 216 LSPLYHTTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMA 275

Query: 292 YSAAIIHNKD-NNPIVYGTVQAYLKDAKDRLLLVAKAADDMKVPLGIKLVRGAYMSSESK 351
           Y +AI+ N D + PIVY T+QAYLKDA +RL L  + ++ M VP+G KLVRGAYMSSE+K
Sbjct: 276 YWSAIMFNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAK 335

Query: 352 IASSLGFESPIHNSIQETHSCYNSCASFLLDNIANGSS-GVILATHNVESGKLAASKAYD 411
           +A SLG++SP+H++IQ TH CYN C SFL++  +NGS   VILATHN +SGKL A KA +
Sbjct: 336 LADSLGYKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASE 395

Query: 412 IGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLL 471
           +GI K   K+EFAQLYGMS+ALSFGL+ AGF VSKYMP+GPVD  +PYL+RRA ENRG++
Sbjct: 396 LGINKENGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMM 455

Query: 472 STSNLDRQLMRKEMARRV 487
           ST  LDRQLMRKE+ RRV
Sbjct: 456 STGALDRQLMRKELKRRV 473