MC04g1704 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g1704
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Calcium-binding EF-hand family protein
Location	MC04: 24544803 .. 24545345 (+)
RNA-Seq Expression	MC04g1704
Synteny	MC04g1704

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTGAATCTGTACAACCTTTCAATGGCGGAGAAAACCGATCGCCCCGAAAATCCCTCTCTCCGCTTCCTTATAATGATCGATTGCCTCGTGTTTCAGAAACTAACCTCGTGGCTCAGCGCCACATACCACGGCTTCGCCTCCACAAGAAATTCAAATCGTTGTTCCCCCATTCCGAAAGCGGTCGTAGACGGCGGCGAGAGTGTGAGCAGAGAGGAGATGAAATCGGTGATGGGAGATTTGGGGATTGTTTGCGGCGAGGAGGGCGATGAATCGCTTCCTCTGTTCGGATCTGTTGAGGAAATCGCGAAGCTGTTCGAGGAGAGAGGGCCGTGTATTGCGGAGGTGAAGAGAGCTTTCGACGTTTTCGACGCCAATGGCGATGGATTCATCGATGTGGTGGAGCTTCAGAGAGTTCTCTGCGTTTTGGGCCTCAAAGAAGGGGAGGGGGTTGAGAATTGCAGGAGGATGATCCAGAAGTTTGATGAAAATGAAGATGGCAAAATTGATTTTGAAGAGTTTGTTAAGTTGATGGAGTCT

mRNA sequence

ATGGATCTGAATCTGTACAACCTTTCAATGGCGGAGAAAACCGATCGCCCCGAAAATCCCTCTCTCCGCTTCCTTATAATGATCGATTGCCTCGTGTTTCAGAAACTAACCTCGTGGCTCAGCGCCACATACCACGGCTTCGCCTCCACAAGAAATTCAAATCGTTGTTCCCCCATTCCGAAAGCGGTCGTAGACGGCGGCGAGAGTGTGAGCAGAGAGGAGATGAAATCGGTGATGGGAGATTTGGGGATTGTTTGCGGCGAGGAGGGCGATGAATCGCTTCCTCTGTTCGGATCTGTTGAGGAAATCGCGAAGCTGTTCGAGGAGAGAGGGCCGTGTATTGCGGAGGTGAAGAGAGCTTTCGACGTTTTCGACGCCAATGGCGATGGATTCATCGATGTGGTGGAGCTTCAGAGAGTTCTCTGCGTTTTGGGCCTCAAAGAAGGGGAGGGGGTTGAGAATTGCAGGAGGATGATCCAGAAGTTTGATGAAAATGAAGATGGCAAAATTGATTTTGAAGAGTTTGTTAAGTTGATGGAGTCT

Coding sequence (CDS)

ATGGATCTGAATCTGTACAACCTTTCAATGGCGGAGAAAACCGATCGCCCCGAAAATCCCTCTCTCCGCTTCCTTATAATGATCGATTGCCTCGTGTTTCAGAAACTAACCTCGTGGCTCAGCGCCACATACCACGGCTTCGCCTCCACAAGAAATTCAAATCGTTGTTCCCCCATTCCGAAAGCGGTCGTAGACGGCGGCGAGAGTGTGAGCAGAGAGGAGATGAAATCGGTGATGGGAGATTTGGGGATTGTTTGCGGCGAGGAGGGCGATGAATCGCTTCCTCTGTTCGGATCTGTTGAGGAAATCGCGAAGCTGTTCGAGGAGAGAGGGCCGTGTATTGCGGAGGTGAAGAGAGCTTTCGACGTTTTCGACGCCAATGGCGATGGATTCATCGATGTGGTGGAGCTTCAGAGAGTTCTCTGCGTTTTGGGCCTCAAAGAAGGGGAGGGGGTTGAGAATTGCAGGAGGATGATCCAGAAGTTTGATGAAAATGAAGATGGCAAAATTGATTTTGAAGAGTTTGTTAAGTTGATGGAGTCT

Protein sequence

MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLMES

Homology

[Close]

BLAST of MC04g1704 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.9e-26
Identity = 61/117 (52.14%), Postives = 84/117 (71.79%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D  + + RE++  VM  LG+   +E +E L    S +E++ LFEE+ P + EVK+AFDVF
Sbjct: 86  DDDDGLCREDVGMVMKSLGLSTDQE-NEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVF 145

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLMES 182
           D N DGFID ++LQRVL +LGLK+G  +ENCRRMI+ FD ++DG+IDF  FVK ME+
Sbjct: 146 DENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFMEN 201

[Close]

BLAST of MC04g1704 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.0e-24
Identity = 60/117 (51.28%), Postives = 78/117 (66.67%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D    +SREE + VM  LG+      D+ L    S +E++ LFEE+   + EVK+AFDVF
Sbjct: 78  DDDIDISREEAEMVMRSLGLF---YNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVF 137

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLMES 182
           D N DGFID +ELQRVL +LG K+G  ++NC  MI+  D N+DGKIDF EFVK ME+
Sbjct: 138 DENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMET 191

[Close]

BLAST of MC04g1704 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.7e-14
Identity = 51/141 (36.17%), Postives = 72/141 (51.06%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFG----------SVEEIAKLF------- 124
           +G   ++REE++  +G LGI      DE   +             VEE  +L+       
Sbjct: 103 NGDGRITREELEDSLGKLGIPV--PADELAAVIARIDANGDGCVDVEEFGELYRSIMAGG 162

Query: 125 ---------EERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRM 180
                    EE      +++ AF VFDANGDG+I V EL  VL  LGLK+G   E CRRM
Sbjct: 163 DDSKDGRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQGRTAEECRRM 222

[Close]

BLAST of MC04g1704 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 8.2e-14
Identity = 61/150 (40.67%), Postives = 85/150 (56.67%), Query Frame = 0


Query: 39  WLSATYHGFASTRNSNRCSPIP---KAVVDGGESV--SREEMKSVMGD---LGIVCGEEG 98
           + S  Y  F S RN+ + + +P   K VV     +  S EE+K+++ D   L     EEG
Sbjct: 42  FFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEG 101

Query: 99  DESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGE 158
           +E L     +E+   + +E       VK AF +FD N DGFID  EL+ VL +LG  E  
Sbjct: 102 EEYL-----LEKNEMMGKE------IVKEAFRLFDENQDGFIDENELKHVLSLLGYDECT 161

Query: 159 GVENCRRMIQKFDENEDGKIDFEEFVKLME 181
            +E CR+M++ +DEN DGKIDF EFVKL+E
Sbjct: 162 KME-CRKMVKVYDENRDGKIDFYEFVKLIE 179

[Close]

BLAST of MC04g1704 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-13
Identity = 45/130 (34.62%), Postives = 75/130 (57.69%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEE------------GDESLPL--FGSVEEIAKLFEER 124
           DG   ++ +E+     +LGI+  E+            GD  + +  FG + +   + +E 
Sbjct: 16  DGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTIMVEDED 75

Query: 125 GPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKI 181
                ++K AF+VFD NGDGFI V EL+ VL  LGLK+G+ +E CR+MI + D + DG++
Sbjct: 76  EVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRV 135

[Close]

BLAST of MC04g1704 vs. NCBI nr
Match: XP_022133625.1 (probable calcium-binding protein CML45 [Momordica charantia])

HSP 1 Score: 361 bits (927), Expect = 8.49e-126
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP
Sbjct: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60

Query: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120
           KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA
Sbjct: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120

Query: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLME 180
           FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENC+RMIQKFDENEDGKIDFEEFVKLME
Sbjct: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME 180

[Close]

BLAST of MC04g1704 vs. NCBI nr
Match: XP_038889669.1 (probable calcium-binding protein CML46 [Benincasa hispida])

HSP 1 Score: 199 bits (506), Expect = 9.00e-62
Identity = 111/184 (60.33%), Postives = 133/184 (72.28%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           MD  +YN+SM +KT   +NPS RFLI+ID L FQKL SWL A    FA+T+      P  
Sbjct: 1   MDFGVYNVSMDDKTTT-QNPSFRFLIVIDFLRFQKLASWLYA----FATTKQKPSSFPAT 60

Query: 61  -KAVVDGGES--VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEV 120
            K    GGE   ++ EEMK VMG+LGI CGE   ES+PLFGS EE+A++F+ER     EV
Sbjct: 61  SKTEEHGGEERLMTTEEMKWVMGNLGIDCGE---ESVPLFGSTEEVAEMFDERAASTVEV 120

Query: 121 KRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVK 180
           K+AFDVFD NGDGFID  ELQRV+CVLG KEGE ++NC RMI+KFD N DG+IDFEEF+K
Sbjct: 121 KKAFDVFDVNGDGFIDSEELQRVMCVLGFKEGERIQNCERMIRKFDHNGDGRIDFEEFLK 176

[Close]

BLAST of MC04g1704 vs. NCBI nr
Match: XP_022993675.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 196 bits (499), Expect = 9.46e-61
Identity = 106/170 (62.35%), Postives = 121/170 (71.18%), Query Frame = 0


Query: 17  PENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAVV-----DGGESVS 76
           P NPS RFLIMIDC  FQKL SWL A    FAS +      P  +        +GGE VS
Sbjct: 7   PANPSFRFLIMIDCFRFQKLGSWLYA----FASGKRKQWSLPASETAKSEQRKEGGERVS 66

Query: 77  REEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGF 136
           REEMK VM +LG+  GEE +ESLP F S+EE+ K+F++     AEVK AFDVFD N DGF
Sbjct: 67  REEMKWVMEELGLSWGEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDVNSDGF 126

Query: 137 IDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLMES 181
           IDV ELQRVLCVLG KEGE ++NCRRMI KFDEN DG+IDF+EFVKLMES
Sbjct: 127 IDVEELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMES 172

[Close]

BLAST of MC04g1704 vs. NCBI nr
Match: XP_008459700.2 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 193 bits (491), Expect = 1.88e-59
Identity = 108/186 (58.06%), Postives = 132/186 (70.97%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           M+  LYN+SM +K+  P+NPS RFLI+ID L FQ L SWL +    FAST+      P  
Sbjct: 1   MNFGLYNVSMDDKST-PQNPSFRFLIIIDFLRFQNLASWLCS----FASTKLKQSTLPAS 60

Query: 61  KAVVDG--GES---VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIA 120
              V+   GE    VS EEMK VMG+LG+ C E    S+PLFGS EE+A++F++     A
Sbjct: 61  SKSVESSAGERERLVSTEEMKWVMGNLGMDCSE--GSSVPLFGSAEEVAEMFDQTAASAA 120

Query: 121 EVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEF 180
           EVK+AFDVFD N DGFIDV ELQRV+CVLG KEGEG+ENC +MI KFD N+DG+IDFEEF
Sbjct: 121 EVKQAFDVFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEF 179

[Close]

BLAST of MC04g1704 vs. NCBI nr
Match: KAG6578574.1 (putative calcium-binding protein CML46, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 190 bits (483), Expect = 2.55e-58
Identity = 103/170 (60.59%), Postives = 120/170 (70.59%), Query Frame = 0


Query: 17  PENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAVV-----DGGESVS 76
           P NPS RFLIMIDC  FQKL SWL A    FA+ +      P  +        +GGE VS
Sbjct: 7   PANPSFRFLIMIDCFRFQKLGSWLYA----FATGKRKQWSLPASETGKSEQRDEGGERVS 66

Query: 77  REEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGF 136
           REEMK VMG+LG+   EE +ESLP F S+EE+ ++F++     AEVK AFDVFD N DGF
Sbjct: 67  REEMKWVMGELGLSSEEETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDVNSDGF 126

Query: 137 IDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLMES 181
           ID  ELQRVLCVLG KEGE ++NCRRMI KFDEN DG+IDF+EFVKLMES
Sbjct: 127 IDGDELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMES 172

[Close]

BLAST of MC04g1704 vs. ExPASy TrEMBL
Match: A0A6J1BVS4 (probable calcium-binding protein CML45 OS=Momordica charantia OX=3673 GN=LOC111006164 PE=4 SV=1)

HSP 1 Score: 361 bits (927), Expect = 4.11e-126
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP
Sbjct: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60

Query: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120
           KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA
Sbjct: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120

Query: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLME 180
           FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENC+RMIQKFDENEDGKIDFEEFVKLME
Sbjct: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME 180

[Close]

BLAST of MC04g1704 vs. ExPASy TrEMBL
Match: A0A6J1JZ70 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111489598 PE=4 SV=1)

HSP 1 Score: 196 bits (499), Expect = 4.58e-61
Identity = 106/170 (62.35%), Postives = 121/170 (71.18%), Query Frame = 0


Query: 17  PENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAVV-----DGGESVS 76
           P NPS RFLIMIDC  FQKL SWL A    FAS +      P  +        +GGE VS
Sbjct: 7   PANPSFRFLIMIDCFRFQKLGSWLYA----FASGKRKQWSLPASETAKSEQRKEGGERVS 66

Query: 77  REEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGF 136
           REEMK VM +LG+  GEE +ESLP F S+EE+ K+F++     AEVK AFDVFD N DGF
Sbjct: 67  REEMKWVMEELGLSWGEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDVNSDGF 126

Query: 137 IDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLMES 181
           IDV ELQRVLCVLG KEGE ++NCRRMI KFDEN DG+IDF+EFVKLMES
Sbjct: 127 IDVEELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMES 172

[Close]

BLAST of MC04g1704 vs. ExPASy TrEMBL
Match: A0A0A0KS94 (Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1)

HSP 1 Score: 194 bits (494), Expect = 3.09e-60
Identity = 107/184 (58.15%), Postives = 134/184 (72.83%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           M+L LYN+SM +K+  P+NPS RFL++ID L FQ L SWL ++     ST+      P  
Sbjct: 2   MNLGLYNVSMDDKST-PQNPSFRFLLIIDFLRFQNLASWLCSS----TSTKLKQSTPPAS 61

Query: 61  -KAVVDGGES--VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEV 120
            K+V    E   VS EEMK VMG+LGI C E    S+PLFGS EE+A++F++    +AEV
Sbjct: 62  SKSVESRAEERLVSTEEMKWVMGNLGIDCSE--GSSVPLFGSAEEVAEMFDQTAASVAEV 121

Query: 121 KRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVK 180
           K+AF VFD NGDGFIDV ELQRV+CVLG KEGEG+ENC +MI+KFD N+DG+IDFEEFVK
Sbjct: 122 KQAFGVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVK 178

[Close]

BLAST of MC04g1704 vs. ExPASy TrEMBL
Match: A0A1S3CAB3 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 PE=4 SV=1)

HSP 1 Score: 193 bits (491), Expect = 9.11e-60
Identity = 108/186 (58.06%), Postives = 132/186 (70.97%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           M+  LYN+SM +K+  P+NPS RFLI+ID L FQ L SWL +    FAST+      P  
Sbjct: 1   MNFGLYNVSMDDKST-PQNPSFRFLIIIDFLRFQNLASWLCS----FASTKLKQSTLPAS 60

Query: 61  KAVVDG--GES---VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIA 120
              V+   GE    VS EEMK VMG+LG+ C E    S+PLFGS EE+A++F++     A
Sbjct: 61  SKSVESSAGERERLVSTEEMKWVMGNLGMDCSE--GSSVPLFGSAEEVAEMFDQTAASAA 120

Query: 121 EVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEF 180
           EVK+AFDVFD N DGFIDV ELQRV+CVLG KEGEG+ENC +MI KFD N+DG+IDFEEF
Sbjct: 121 EVKQAFDVFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEF 179

[Close]

BLAST of MC04g1704 vs. ExPASy TrEMBL
Match: A0A6J1FKT4 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111445127 PE=4 SV=1)

HSP 1 Score: 188 bits (477), Expect = 1.00e-57
Identity = 102/170 (60.00%), Postives = 119/170 (70.00%), Query Frame = 0


Query: 17  PENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAVV-----DGGESVS 76
           P  PS RFLIMIDC  FQKL SWL A    FA+ +      P  +        +GGE VS
Sbjct: 7   PAIPSFRFLIMIDCFRFQKLGSWLYA----FATGKRKQWSLPASETAKSEQRDEGGERVS 66

Query: 77  REEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGF 136
           REEMK VMG+LG+   EE +ESLP F S+EE+ ++F++     AEVK AFDVFD N DGF
Sbjct: 67  REEMKWVMGELGLSSEEETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDVNSDGF 126

Query: 137 IDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLMES 181
           ID  ELQRVLCVLG KEGE ++NCRRMI KFDEN DG+IDF+EFVKLMES
Sbjct: 127 IDGDELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMES 172

[Close]

BLAST of MC04g1704 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.3e-27
Identity = 61/117 (52.14%), Postives = 84/117 (71.79%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D  + + RE++  VM  LG+   +E +E L    S +E++ LFEE+ P + EVK+AFDVF
Sbjct: 86  DDDDGLCREDVGMVMKSLGLSTDQE-NEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVF 145

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLMES 182
           D N DGFID ++LQRVL +LGLK+G  +ENCRRMI+ FD ++DG+IDF  FVK ME+
Sbjct: 146 DENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFMEN 201

[Close]

BLAST of MC04g1704 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 114.8 bits (286), Expect = 7.4e-26
Identity = 60/117 (51.28%), Postives = 78/117 (66.67%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D    +SREE + VM  LG+      D+ L    S +E++ LFEE+   + EVK+AFDVF
Sbjct: 78  DDDIDISREEAEMVMRSLGLF---YNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVF 137

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFEEFVKLMES 182
           D N DGFID +ELQRVL +LG K+G  ++NC  MI+  D N+DGKIDF EFVK ME+
Sbjct: 138 DENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMET 191

[Close]

BLAST of MC04g1704 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 78.6 bits (192), Expect = 5.9e-15
Identity = 61/150 (40.67%), Postives = 85/150 (56.67%), Query Frame = 0


Query: 39  WLSATYHGFASTRNSNRCSPIP---KAVVDGGESV--SREEMKSVMGD---LGIVCGEEG 98
           + S  Y  F S RN+ + + +P   K VV     +  S EE+K+++ D   L     EEG
Sbjct: 42  FFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEG 101

Query: 99  DESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGE 158
           +E L     +E+   + +E       VK AF +FD N DGFID  EL+ VL +LG  E  
Sbjct: 102 EEYL-----LEKNEMMGKE------IVKEAFRLFDENQDGFIDENELKHVLSLLGYDECT 161

Query: 159 GVENCRRMIQKFDENEDGKIDFEEFVKLME 181
            +E CR+M++ +DEN DGKIDF EFVKL+E
Sbjct: 162 KME-CRKMVKVYDENRDGKIDFYEFVKLIE 179

[Close]

BLAST of MC04g1704 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 77.8 bits (190), Expect = 1.0e-14
Identity = 45/130 (34.62%), Postives = 75/130 (57.69%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEE------------GDESLPL--FGSVEEIAKLFEER 124
           DG   ++ +E+     +LGI+  E+            GD  + +  FG + +   + +E 
Sbjct: 16  DGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTIMVEDED 75

Query: 125 GPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKI 181
                ++K AF+VFD NGDGFI V EL+ VL  LGLK+G+ +E CR+MI + D + DG++
Sbjct: 76  EVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRV 135

[Close]

BLAST of MC04g1704 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 75.9 bits (185), Expect = 3.8e-14
Identity = 45/127 (35.43%), Postives = 75/127 (59.06%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEG----DESLPLFG----SVEEIAKLFE---ERGPC 124
           +G   ++++E+   + +LGI   ++      E + L G     +EE   L++   E    
Sbjct: 16  NGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDE 75

Query: 125 IAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCRRMIQKFDENEDGKIDFE 181
             +++ AF+VFD N DGFI V EL+ VL  LGLK+G  +E+C+RMI K D + DG ++F+
Sbjct: 76  EEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFK 135

The following BLAST results are available for this feature:

BLAST of MC04g1704 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93Z27	1.9e-26	52.14	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9MBG5	1.0e-24	51.28	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q0JC44	3.7e-14	36.17	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SN89	8.2e-14	40.67	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]
Q9ZR02	1.4e-13	34.62	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]

BLAST of MC04g1704 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133625.1	8.49e-126	99.45	probable calcium-binding protein CML45 [Momordica charantia]	[more]
XP_038889669.1	9.00e-62	60.33	probable calcium-binding protein CML46 [Benincasa hispida]	[more]
XP_022993675.1	9.46e-61	62.35	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]
XP_008459700.2	1.88e-59	58.06	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
KAG6578574.1	2.55e-58	60.59	putative calcium-binding protein CML46, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of MC04g1704 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVS4	4.11e-126	99.45	probable calcium-binding protein CML45 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1JZ70	4.58e-61	62.35	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114895...	[more]
A0A0A0KS94	3.09e-60	58.15	Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1	[more]
A0A1S3CAB3	9.11e-60	58.06	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 P...	[more]
A0A6J1FKT4	1.00e-57	60.00	probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of MC04g1704 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G39670.1	1.3e-27	52.14	Calcium-binding EF-hand family protein	[more]
AT3G29000.1	7.4e-26	51.28	Calcium-binding EF-hand family protein	[more]
AT3G47480.1	5.9e-15	40.67	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	1.0e-14	34.62	EF hand calcium-binding protein family	[more]
AT3G07490.1	3.8e-14	35.43	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 116..144 e-value: 0.0027 score: 26.9 coord: 154..181 e-value: 0.32 score: 20.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 116..180 e-value: 8.3E-13 score: 48.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 150..181 score: 12.337564
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 112..147 score: 14.262346
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 116..179 e-value: 5.16065E-17 score: 69.4989
None	No IPR available	GENE3D	1.10.238.10		coord: 57..181 e-value: 7.8E-21 score: 76.6
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 58..180
None	No IPR available	PANTHER	PTHR10891:SF805	CALCIUM-BINDING PROTEIN CML45-RELATED	coord: 58..180
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 125..137
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 64..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g1704.1	MC04g1704.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding