MC04g1702 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g1702
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	E3 ubiquitin-protein ligase RZF1-like
Location	MC04: 24500225 .. 24500851 (+)
RNA-Seq Expression	MC04g1702
Synteny	MC04g1702

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCGATTCCGAGTCCTCATCTCAGCCGCCGCGGCCCCGCAGCCTCTCCATGCTTCTCCCCTTTCTTCTTGGCGTTGCGCAGAGCACCAACGGATCGCACCTCAATCAGATCGTCGTTCTCGACCACGCTGCGCGGACGGTTGTGGTTTTCGAAGGCCATGTTCTCGATTCTCTTCTCCGCGACCTATCGGAGAAGACCGGGCCCTTGCCGGCGTCCAAGGATTCTATCGACGCGATGCCGAGGGTGCGGGTTACGGAAGTCGGGACGGATTGTGCGATCTGCTTGGACGGATTCGAAGTCGGTGAGGAGGCCAGAGAGATGCCTTGCAAACACAGGTACCATTCGGAGTGTATTGAGAAGTGGTTGGGGGTTCACGGGACGTGTCCGGTTTGTCGGTTCGCCATGCCGGTGGGGAAGAAGAACGGCGGCGATGATGGTGAAGATATTGAAGGAGGAGAAGGCAGAGTAGTTGTGGTGAATCTGTGGGTTAGCGACGGCGATACAATGGATGATGTTGAATCGGTTTCGGGGATTGGATCAGAGGACTCCGATTCGGAATCGCAAGATGGAATGATGATCGATGTTGATGTCTCATCAACGAGGGATCCCAGTCCTGAAGATTCT

mRNA sequence

ATGTCCGATTCCGAGTCCTCATCTCAGCCGCCGCGGCCCCGCAGCCTCTCCATGCTTCTCCCCTTTCTTCTTGGCGTTGCGCAGAGCACCAACGGATCGCACCTCAATCAGATCGTCGTTCTCGACCACGCTGCGCGGACGGTTGTGGTTTTCGAAGGCCATGTTCTCGATTCTCTTCTCCGCGACCTATCGGAGAAGACCGGGCCCTTGCCGGCGTCCAAGGATTCTATCGACGCGATGCCGAGGGTGCGGGTTACGGAAGTCGGGACGGATTGTGCGATCTGCTTGGACGGATTCGAAGTCGGTGAGGAGGCCAGAGAGATGCCTTGCAAACACAGGTACCATTCGGAGTGTATTGAGAAGTGGTTGGGGGTTCACGGGACGTGTCCGGTTTGTCGGTTCGCCATGCCGGTGGGGAAGAAGAACGGCGGCGATGATGGTGAAGATATTGAAGGAGGAGAAGGCAGAGTAGTTGTGGTGAATCTGTGGGTTAGCGACGGCGATACAATGGATGATGTTGAATCGGTTTCGGGGATTGGATCAGAGGACTCCGATTCGGAATCGCAAGATGGAATGATGATCGATGTTGATGTCTCATCAACGAGGGATCCCAGTCCTGAAGATTCT

Coding sequence (CDS)

ATGTCCGATTCCGAGTCCTCATCTCAGCCGCCGCGGCCCCGCAGCCTCTCCATGCTTCTCCCCTTTCTTCTTGGCGTTGCGCAGAGCACCAACGGATCGCACCTCAATCAGATCGTCGTTCTCGACCACGCTGCGCGGACGGTTGTGGTTTTCGAAGGCCATGTTCTCGATTCTCTTCTCCGCGACCTATCGGAGAAGACCGGGCCCTTGCCGGCGTCCAAGGATTCTATCGACGCGATGCCGAGGGTGCGGGTTACGGAAGTCGGGACGGATTGTGCGATCTGCTTGGACGGATTCGAAGTCGGTGAGGAGGCCAGAGAGATGCCTTGCAAACACAGGTACCATTCGGAGTGTATTGAGAAGTGGTTGGGGGTTCACGGGACGTGTCCGGTTTGTCGGTTCGCCATGCCGGTGGGGAAGAAGAACGGCGGCGATGATGGTGAAGATATTGAAGGAGGAGAAGGCAGAGTAGTTGTGGTGAATCTGTGGGTTAGCGACGGCGATACAATGGATGATGTTGAATCGGTTTCGGGGATTGGATCAGAGGACTCCGATTCGGAATCGCAAGATGGAATGATGATCGATGTTGATGTCTCATCAACGAGGGATCCCAGTCCTGAAGATTCT

Protein sequence

MSDSESSSQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLDSLLRDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSECIEKWLGVHGTCPVCRFAMPVGKKNGGDDGEDIEGGEGRVVVVNLWVSDGDTMDDVESVSGIGSEDSDSESQDGMMIDVDVSSTRDPSPEDS

Homology

[Close]

BLAST of MC04g1702 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.2e-27
Identity = 76/190 (40.00%), Postives = 111/190 (58.42%), Query Frame = 0


Query: 12  RPRSLSMLLPFLLGVA--QSTNG------SHLNQIVVLDHAARTVVVFEGHV-LDSLLRD 71
           R R L + LPFLLG +  +S+NG      S   +I++++   + ++V EG   ++ LLR 
Sbjct: 22  RNRDLYLFLPFLLGFSDQESSNGDDDDVASSRERIILVNPFTQGMIVLEGSSGMNPLLRS 81

Query: 72  L--SEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSECIE 131
           L  S + G  PASK SIDAMP V +     +C ICL+ ++  E  +EMPCKHR+H  CIE
Sbjct: 82  LLESREEGRPPASKASIDAMPIVEIDGCEGECVICLEEWKSEETVKEMPCKHRFHGGCIE 141

Query: 132 KWLGVHGTCPVCRFAMPVGKKNGGDDGEDI--EGGEGRVVVVNLWVSDGDTMDDVESVSG 189
           KWLG HG+CPVCR+ MPV       DG++I  +  +G  + V    +DG  + D  +  G
Sbjct: 142 KWLGFHGSCPVCRYEMPV-------DGDEIGKKRNDGNEIWVRFSFNDGRRIRDFSAQDG 201

[Close]

BLAST of MC04g1702 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-19
Identity = 41/89 (46.07%), Postives = 62/89 (69.66%), Query Frame = 0


Query: 53  GHVLDSLLRDLSE----KTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREM 112
           G  L  LL+ L+E    + G  PA K++++A+P V++ EV   C++CLD  EVG +A++M
Sbjct: 157 GAGLSLLLQHLAESDPSRNGTPPAKKEAVEALPTVKIEEV-VSCSVCLDDLEVGSQAKQM 216

Query: 113 PCKHRYHSECIEKWLGVHGTCPVCRFAMP 138
           PC+H++HS CI  WL +H +CPVCRF +P
Sbjct: 217 PCEHKFHSSCILPWLELHSSCPVCRFELP 244

[Close]

BLAST of MC04g1702 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.4e-19
Identity = 47/121 (38.84%), Postives = 69/121 (57.02%), Query Frame = 0


Query: 51  FEGHVLDSLLRDLSE----KTGPLPASKDSIDAMPRVRVTE-----VGTDCAICLDGFEV 110
           F G  L+ L++ L+E    + G  PASK +IDA+P V+VT+         CA+C+D FE 
Sbjct: 166 FFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFED 225

Query: 111 GEEAREMPCKHRYHSECIEKWLGVHGTCPVCRFAMPV----------GKKNGGDDGEDIE 153
           G + ++MPCKH +H +C+  WL +H +CPVCRF +P           G +  GD    +E
Sbjct: 226 GSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVE 285

[Close]

BLAST of MC04g1702 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 7.5e-19
Identity = 46/120 (38.33%), Postives = 69/120 (57.50%), Query Frame = 0


Query: 51  FEGHVLDSLLRDLSE----KTGPLPASKDSIDAMPRVRVTEVG-----TDCAICLDGFEV 110
           F G  L+ L++ L+E    + G  PASK +I+A+P V +T+         CA+C+D FE 
Sbjct: 174 FIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEE 233

Query: 111 GEEAREMPCKHRYHSECIEKWLGVHGTCPVCRFAMPVGK------------KNGGDDGED 150
           G EA++MPCKH YH +C+  WL +H +CPVCR  +P                +GG+DG++
Sbjct: 234 GTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSGGNDGDN 293

[Close]

BLAST of MC04g1702 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 4.9e-18
Identity = 41/101 (40.59%), Postives = 61/101 (60.40%), Query Frame = 0


Query: 50  VFEGHVLDSLLRDLSE---------KTGPLPASKDSIDAMPRVRVTEVGT----DCAICL 109
           +  G   + LL  LS+         ++G  PASK +I+++PRV +++  T    +CA+C 
Sbjct: 160 ILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCT 219

Query: 110 DGFEVGEEAREMPCKHRYHSECIEKWLGVHGTCPVCRFAMP 138
           + FE G E REMPCKH +H +CI  WL +  +CPVCRF +P
Sbjct: 220 EVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of MC04g1702 vs. NCBI nr
Match: XP_022133881.1 (E3 ubiquitin-protein ligase RZF1-like [Momordica charantia])

HSP 1 Score: 416 bits (1069), Expect = 1.50e-146
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 1   MSDSESSSQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLDSLL 60
           MSDSESSSQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLDSLL
Sbjct: 1   MSDSESSSQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLDSLL 60

Query: 61  RDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSECIE 120
           RDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSECIE
Sbjct: 61  RDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSECIE 120

Query: 121 KWLGVHGTCPVCRFAMPVGKKNGGDDGEDIEGGEGRVVVVNLWVSDGDTMDDVESVSGIG 180
           KWLGVHGTCPVCRFAMPVGKKNGGDDGEDIEGGEGRVVVVNLWVSDGDTMDDVESVSGIG
Sbjct: 121 KWLGVHGTCPVCRFAMPVGKKNGGDDGEDIEGGEGRVVVVNLWVSDGDTMDDVESVSGIG 180

Query: 181 SEDSDSESQDGMMIDVDVSSTRDPSPEDS 209
           SEDSDSESQDGMMIDVDVSSTRDPSPEDS
Sbjct: 181 SEDSDSESQDGMMIDVDVSSTRDPSPEDS 209

[Close]

BLAST of MC04g1702 vs. NCBI nr
Match: KAA0039492.1 (E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK15247.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 302 bits (774), Expect = 3.06e-101
Identity = 168/225 (74.67%), Postives = 188/225 (83.56%), Query Frame = 0


Query: 1   MSDSESS---SQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLD 60
           MSDS+SS   S PP PR+LSMLLPFLLG+A++T GS+LNQIVVLDHAARTVVVFEGHVLD
Sbjct: 1   MSDSDSSPSPSPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVLD 60

Query: 61  SLLRDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSE 120
           SLLRDLSEK+GPLPASK SIDAMPRV VTE+GTDCAICLDGFEV EEAREMPCKH YHS+
Sbjct: 61  SLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHMYHSD 120

Query: 121 CIEKWLGVHGTCPVCRFAMPVG--KKNGG------DDGEDIEGGE---GRVVVVNLWVSD 180
           CIEKWL V GTCPVCRF MPV   +K+GG      ++ ED+EGGE   GRVVVVNLWV+D
Sbjct: 121 CIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNLWVND 180

Query: 181 GDTMDDVESVSGIGSEDSDSESQDGMMIDVDVSSTR--DPSPEDS 209
           G+ MD VES    GSEDSDSESQD +M++VD SST+  DPS EDS
Sbjct: 181 GEPMD-VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDS 224

[Close]

BLAST of MC04g1702 vs. NCBI nr
Match: XP_022993365.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima])

HSP 1 Score: 301 bits (772), Expect = 5.02e-101
Identity = 162/218 (74.31%), Postives = 180/218 (82.57%), Query Frame = 0


Query: 1   MSDSESSSQPPR----PRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVL 60
           MSDS+SSS PP     PRSLSMLLPFLLG+A++T GSHLNQIVVLDHAARTVVVFEGHVL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  DSLLRDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHS 120
           +S LRDLSEK+GPLPASK SIDAMPRV +TE+G DCAICLDGFEVG EAREMPCKH YHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHMYHS 120

Query: 121 ECIEKWLGVHGTCPVCRFAMPVGKKNGG-DDGEDIEGGEGRVVVVNLWVSDGDTMD--DV 180
           +CIEKWL V GTCP+CRF MPV  + G  DD ED+EG EGRVVVVNLWV+DG+ MD   V
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESVSGIGSEDSDSESQDGMMIDVDVSSTR--DPSPEDS 209
           ES  G GS DSDSES D + ++VD SST+  DPS EDS
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDS 218

[Close]

BLAST of MC04g1702 vs. NCBI nr
Match: XP_023551536.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 301 bits (771), Expect = 7.12e-101
Identity = 162/218 (74.31%), Postives = 180/218 (82.57%), Query Frame = 0


Query: 1   MSDSESSSQPPR----PRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVL 60
           MSDS+SSS PP     PRSLSMLLPFLLG+A++T GSHLNQIVVLDHAARTVVVFEGHVL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  DSLLRDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHS 120
           +S LRDLSEK+GPLPASK SIDAMPRV +TE+G DCAICLDGFEVG EAREMPCKH YHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120

Query: 121 ECIEKWLGVHGTCPVCRFAMPVGKKNGG-DDGEDIEGGEGRVVVVNLWVSDGDTMD--DV 180
           +CIEKWL V GTCP+CRF MPV  + G  DD ED+EG EGRVVVVNLWV+DG+ MD   V
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESVSGIGSEDSDSESQDGMMIDVDVSSTR--DPSPEDS 209
           ES  G GS DSDSES D + ++VD SST+  DPS EDS
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDS 218

[Close]

BLAST of MC04g1702 vs. NCBI nr
Match: XP_011656279.1 (E3 ubiquitin-protein ligase MPSR1 [Cucumis sativus] >KGN52465.1 hypothetical protein Csa_008626 [Cucumis sativus])

HSP 1 Score: 301 bits (770), Expect = 1.52e-100
Identity = 169/231 (73.16%), Postives = 185/231 (80.09%), Query Frame = 0


Query: 1   MSDSESS---SQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLD 60
           MSDS+SS   S PP PR+LSMLLPFLLG+A++T GS+LNQIVVLDHAARTVVVFEGHVLD
Sbjct: 1   MSDSDSSPSPSPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVLD 60

Query: 61  SLLRDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSE 120
           SLLRDLSEK+GPLPASK SIDAMPRV VTE+G DCAICLDGFEV EEAREMPCKH YHS+
Sbjct: 61  SLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHMYHSD 120

Query: 121 CIEKWLGVHGTCPVCRFAMPV-------GKKNGGDD--------GEDIEGGEGRVVVVNL 180
           CIEKWL V GTCPVCRFAMPV       G   GGD+        GE  EGGEGRVVVVNL
Sbjct: 121 CIEKWLNVRGTCPVCRFAMPVDDRRKDGGGGGGGDEEEEEDMEGGEGGEGGEGRVVVVNL 180

Query: 181 WVSDGDTMDDVESVSGIGSEDSDSESQDGMMIDVDVSSTR----DPSPEDS 209
           WV+DG+ MD VES    GSEDSDSESQD +M++VD SST+    DPS EDS
Sbjct: 181 WVNDGEPMD-VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQEQDPSSEDS 230

[Close]

BLAST of MC04g1702 vs. ExPASy TrEMBL
Match: A0A6J1BWE8 (E3 ubiquitin-protein ligase RZF1-like OS=Momordica charantia OX=3673 GN=LOC111006326 PE=4 SV=1)

HSP 1 Score: 416 bits (1069), Expect = 7.24e-147
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 1   MSDSESSSQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLDSLL 60
           MSDSESSSQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLDSLL
Sbjct: 1   MSDSESSSQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLDSLL 60

Query: 61  RDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSECIE 120
           RDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSECIE
Sbjct: 61  RDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSECIE 120

Query: 121 KWLGVHGTCPVCRFAMPVGKKNGGDDGEDIEGGEGRVVVVNLWVSDGDTMDDVESVSGIG 180
           KWLGVHGTCPVCRFAMPVGKKNGGDDGEDIEGGEGRVVVVNLWVSDGDTMDDVESVSGIG
Sbjct: 121 KWLGVHGTCPVCRFAMPVGKKNGGDDGEDIEGGEGRVVVVNLWVSDGDTMDDVESVSGIG 180

Query: 181 SEDSDSESQDGMMIDVDVSSTRDPSPEDS 209
           SEDSDSESQDGMMIDVDVSSTRDPSPEDS
Sbjct: 181 SEDSDSESQDGMMIDVDVSSTRDPSPEDS 209

[Close]

BLAST of MC04g1702 vs. ExPASy TrEMBL
Match: A0A5A7T7P7 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00210 PE=4 SV=1)

HSP 1 Score: 302 bits (774), Expect = 1.48e-101
Identity = 168/225 (74.67%), Postives = 188/225 (83.56%), Query Frame = 0


Query: 1   MSDSESS---SQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLD 60
           MSDS+SS   S PP PR+LSMLLPFLLG+A++T GS+LNQIVVLDHAARTVVVFEGHVLD
Sbjct: 1   MSDSDSSPSPSPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVLD 60

Query: 61  SLLRDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSE 120
           SLLRDLSEK+GPLPASK SIDAMPRV VTE+GTDCAICLDGFEV EEAREMPCKH YHS+
Sbjct: 61  SLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHMYHSD 120

Query: 121 CIEKWLGVHGTCPVCRFAMPVG--KKNGG------DDGEDIEGGE---GRVVVVNLWVSD 180
           CIEKWL V GTCPVCRF MPV   +K+GG      ++ ED+EGGE   GRVVVVNLWV+D
Sbjct: 121 CIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNLWVND 180

Query: 181 GDTMDDVESVSGIGSEDSDSESQDGMMIDVDVSSTR--DPSPEDS 209
           G+ MD VES    GSEDSDSESQD +M++VD SST+  DPS EDS
Sbjct: 181 GEPMD-VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDS 224

[Close]

BLAST of MC04g1702 vs. ExPASy TrEMBL
Match: A0A6J1JYB9 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111489408 PE=4 SV=1)

HSP 1 Score: 301 bits (772), Expect = 2.43e-101
Identity = 162/218 (74.31%), Postives = 180/218 (82.57%), Query Frame = 0


Query: 1   MSDSESSSQPPR----PRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVL 60
           MSDS+SSS PP     PRSLSMLLPFLLG+A++T GSHLNQIVVLDHAARTVVVFEGHVL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  DSLLRDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHS 120
           +S LRDLSEK+GPLPASK SIDAMPRV +TE+G DCAICLDGFEVG EAREMPCKH YHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHMYHS 120

Query: 121 ECIEKWLGVHGTCPVCRFAMPVGKKNGG-DDGEDIEGGEGRVVVVNLWVSDGDTMD--DV 180
           +CIEKWL V GTCP+CRF MPV  + G  DD ED+EG EGRVVVVNLWV+DG+ MD   V
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESVSGIGSEDSDSESQDGMMIDVDVSSTR--DPSPEDS 209
           ES  G GS DSDSES D + ++VD SST+  DPS EDS
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDS 218

[Close]

BLAST of MC04g1702 vs. ExPASy TrEMBL
Match: A0A0A0KS87 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G636570 PE=4 SV=1)

HSP 1 Score: 301 bits (770), Expect = 7.38e-101
Identity = 169/231 (73.16%), Postives = 185/231 (80.09%), Query Frame = 0


Query: 1   MSDSESS---SQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLD 60
           MSDS+SS   S PP PR+LSMLLPFLLG+A++T GS+LNQIVVLDHAARTVVVFEGHVLD
Sbjct: 1   MSDSDSSPSPSPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVLD 60

Query: 61  SLLRDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSE 120
           SLLRDLSEK+GPLPASK SIDAMPRV VTE+G DCAICLDGFEV EEAREMPCKH YHS+
Sbjct: 61  SLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHMYHSD 120

Query: 121 CIEKWLGVHGTCPVCRFAMPV-------GKKNGGDD--------GEDIEGGEGRVVVVNL 180
           CIEKWL V GTCPVCRFAMPV       G   GGD+        GE  EGGEGRVVVVNL
Sbjct: 121 CIEKWLNVRGTCPVCRFAMPVDDRRKDGGGGGGGDEEEEEDMEGGEGGEGGEGRVVVVNL 180

Query: 181 WVSDGDTMDDVESVSGIGSEDSDSESQDGMMIDVDVSSTR----DPSPEDS 209
           WV+DG+ MD VES    GSEDSDSESQD +M++VD SST+    DPS EDS
Sbjct: 181 WVNDGEPMD-VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQEQDPSSEDS 230

[Close]

BLAST of MC04g1702 vs. ExPASy TrEMBL
Match: A0A1S3CC03 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103498739 PE=4 SV=1)

HSP 1 Score: 299 bits (766), Expect = 1.92e-100
Identity = 163/220 (74.09%), Postives = 184/220 (83.64%), Query Frame = 0


Query: 1   MSDSESS---SQPPRPRSLSMLLPFLLGVAQSTNGSHLNQIVVLDHAARTVVVFEGHVLD 60
           MSDS+SS   S PP PR+LSMLLPFLLG+A++T GS+LNQIVVLDHAARTVVVFEGHVLD
Sbjct: 1   MSDSDSSPSPSPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVLD 60

Query: 61  SLLRDLSEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSE 120
           SLLRDLSEK+GPLPASK SIDAMPRV VTE+GTDCAICLDGFEV EEAREMPCKH YHS+
Sbjct: 61  SLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHMYHSD 120

Query: 121 CIEKWLGVHGTCPVCRFAMPVG--KKNGG------DDGEDIEGGE---GRVVVVNLWVSD 180
           CIEKWL V GTCPVCRF MPV   +K+GG      ++ ED+EGGE   GRVVVVNLWV+D
Sbjct: 121 CIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNLWVND 180

Query: 181 GDTMDDVESVSGIGSEDSDSESQDGMMIDVDVSSTRDPSP 206
           G+ MD VES    GSEDSDSESQD +M++VD SST++  P
Sbjct: 181 GEPMD-VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDP 219

[Close]

BLAST of MC04g1702 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 8.2e-29
Identity = 76/190 (40.00%), Postives = 111/190 (58.42%), Query Frame = 0


Query: 12  RPRSLSMLLPFLLGVA--QSTNG------SHLNQIVVLDHAARTVVVFEGHV-LDSLLRD 71
           R R L + LPFLLG +  +S+NG      S   +I++++   + ++V EG   ++ LLR 
Sbjct: 22  RNRDLYLFLPFLLGFSDQESSNGDDDDVASSRERIILVNPFTQGMIVLEGSSGMNPLLRS 81

Query: 72  L--SEKTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAREMPCKHRYHSECIE 131
           L  S + G  PASK SIDAMP V +     +C ICL+ ++  E  +EMPCKHR+H  CIE
Sbjct: 82  LLESREEGRPPASKASIDAMPIVEIDGCEGECVICLEEWKSEETVKEMPCKHRFHGGCIE 141

Query: 132 KWLGVHGTCPVCRFAMPVGKKNGGDDGEDI--EGGEGRVVVVNLWVSDGDTMDDVESVSG 189
           KWLG HG+CPVCR+ MPV       DG++I  +  +G  + V    +DG  + D  +  G
Sbjct: 142 KWLGFHGSCPVCRYEMPV-------DGDEIGKKRNDGNEIWVRFSFNDGRRIRDFSAQDG 201

[Close]

BLAST of MC04g1702 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 1.7e-21
Identity = 56/145 (38.62%), Postives = 82/145 (56.55%), Query Frame = 0


Query: 51  FEGHVLDSLLRDLSE----KTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAR 110
           F G  LD LL+ L+E    + G  PA K++++A+P V++ E    C++CLD FE G EA+
Sbjct: 215 FIGPGLDLLLQHLAENDPNRQGTPPARKEAVEALPTVKIME-PLQCSVCLDDFEKGTEAK 274

Query: 111 EMPCKHRYHSECIEKWLGVHGTCPVCRFAMPVGKKNGGDDGEDIEGGEGRV--------- 170
           EMPCKH++H  CI  WL +H +CPVCRF +P    +  DD ++ +    RV         
Sbjct: 275 EMPCKHKFHVRCIVPWLELHSSCPVCRFELP----SSADDDDETKTDSERVLRTRNVRET 334

Query: 171 ----VVVNLWVSDGDTMDDVESVSG 179
               VV N+  +D    D+V S +G
Sbjct: 335 SNGNVVENVGNADRGREDEVRSGNG 354

[Close]

BLAST of MC04g1702 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.8e-21
Identity = 50/129 (38.76%), Postives = 74/129 (57.36%), Query Frame = 0


Query: 51  FEGHVLDSLLRDLSE----KTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAR 110
           F G  LD LL  L++    + G LPA K+ +D +P V+++E    C+ICLD F+ G EA+
Sbjct: 197 FVGSSLDHLLEHLADNDSIRHGSLPARKEVVDNLPTVKISE-SLQCSICLDDFDKGSEAK 256

Query: 111 EMPCKHRYHSECIEKWLGVHGTCPVCRFAMPVGKKNGGDDGEDIEGGEGRVVVVNLWVSD 170
           EMPCKH++H  CI  WL +H +CPVCR+ +P       DD   +     R   + + VS+
Sbjct: 257 EMPCKHKFHIRCIVPWLELHSSCPVCRYELP------PDDETKVNPVRPRTRTLEINVSN 316

Query: 171 GDTMDDVES 176
            +  DD  +
Sbjct: 317 ENVEDDARN 318

[Close]

BLAST of MC04g1702 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.8e-21
Identity = 50/129 (38.76%), Postives = 74/129 (57.36%), Query Frame = 0


Query: 51  FEGHVLDSLLRDLSE----KTGPLPASKDSIDAMPRVRVTEVGTDCAICLDGFEVGEEAR 110
           F G  LD LL  L++    + G LPA K+ +D +P V+++E    C+ICLD F+ G EA+
Sbjct: 197 FVGSSLDHLLEHLADNDSIRHGSLPARKEVVDNLPTVKISE-SLQCSICLDDFDKGSEAK 256

Query: 111 EMPCKHRYHSECIEKWLGVHGTCPVCRFAMPVGKKNGGDDGEDIEGGEGRVVVVNLWVSD 170
           EMPCKH++H  CI  WL +H +CPVCR+ +P       DD   +     R   + + VS+
Sbjct: 257 EMPCKHKFHIRCIVPWLELHSSCPVCRYELP------PDDETKVNPVRPRTRTLEINVSN 316

Query: 171 GDTMDDVES 176
            +  DD  +
Sbjct: 317 ENVEDDARN 318

[Close]

BLAST of MC04g1702 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-20
Identity = 53/120 (44.17%), Postives = 68/120 (56.67%), Query Frame = 0


Query: 46  RTVVVFEGHVLDSLLRDLSEKTGPLPASKDSIDAMPRVRVTE----VGTDCAICLDGFEV 105
           R V++     LD    D   K G  PA K  ++ MPRV + E     G  CAICLD +  
Sbjct: 60  RFVMIGSRSGLDDFFSD-GGKQGRSPALKSEVENMPRVVIGEDKEKYGGSCAICLDEWSK 119

Query: 106 GEEAREMPCKHRYHSECIEKWLGVHGTCPVCRFAMPV-----GKKNGGDDGEDIEGGEGR 157
           G+ A EMPCKH++HS+C+E+WLG H TCP+CR+ MPV      KK G   G  I  G+ R
Sbjct: 120 GDVAAEMPCKHKFHSKCVEEWLGRHATCPMCRYEMPVEEVEEEKKIGIWIGFSINAGDRR 178

The following BLAST results are available for this feature:

BLAST of MC04g1702 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	1.2e-27	40.00	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q6AVN2	1.2e-19	46.07	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8LPN7	3.4e-19	38.84	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	7.5e-19	38.33	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q9SNB6	4.9e-18	40.59	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]

BLAST of MC04g1702 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133881.1	1.50e-146	100.00	E3 ubiquitin-protein ligase RZF1-like [Momordica charantia]	[more]
KAA0039492.1	3.06e-101	74.67	E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK15247.1 E3...	[more]
XP_022993365.1	5.02e-101	74.31	E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima]	[more]
XP_023551536.1	7.12e-101	74.31	E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo]	[more]
XP_011656279.1	1.52e-100	73.16	E3 ubiquitin-protein ligase MPSR1 [Cucumis sativus] >KGN52465.1 hypothetical pro...	[more]

BLAST of MC04g1702 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BWE8	7.24e-147	100.00	E3 ubiquitin-protein ligase RZF1-like OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A5A7T7P7	1.48e-101	74.67	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1JYB9	2.43e-101	74.31	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]
A0A0A0KS87	7.38e-101	73.16	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G636570 P...	[more]
A0A1S3CC03	1.92e-100	74.09	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103498739 P...	[more]

BLAST of MC04g1702 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26800.1	8.2e-29	40.00	RING/U-box superfamily protein	[more]
AT5G56340.1	1.7e-21	38.62	RING/U-box superfamily protein	[more]
AT4G26400.2	2.8e-21	38.76	RING/U-box superfamily protein	[more]
AT4G26400.1	2.8e-21	38.76	RING/U-box superfamily protein	[more]
AT1G14200.1	1.1e-20	44.17	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 92..132 e-value: 2.4E-8 score: 43.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 91..133 e-value: 1.5E-14 score: 54.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 92..133 score: 12.267982
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 60..139 e-value: 2.7E-22 score: 80.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 169..209
None	No IPR available	PANTHER	PTHR15710:SF132	E3 UBIQUITIN-PROTEIN LIGASE MPSR1	coord: 36..146
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 36..146
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 91..133 e-value: 3.04666E-22 score: 83.1201
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 91..138

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g1702.1	MC04g1702.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding