MC04g1701 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g1701
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Defective in cullin neddylation protein
Location	MC04: 24491436 .. 24494331 (-)
RNA-Seq Expression	MC04g1701
Synteny	MC04g1701

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTATAGATATTCGATCTCAAAATGGCTTTTGTTGTGGGAATGAAGACTCCAGACATGTCGAAACACAAATAGCTAAAAGTTCAAAGGAGGCACTTGCAGCGCTCTTAAAATATGTGCAATCATCACTGCAGATGGGGTACATCCCTTAAAACCTATACAATAGGCCTCGTTATGTTTTGTTAGTTACACTATGTATCACATGGACCATGATAGTATAGGATTGGATATCTAGAGTTTTACACGGTTTTATAAAACTGAATAATTTTTGTCCTTCCAAAATAAGACATTTTTTGACAGATGTGTAACTAAGATAAATTATCAACTAAATTTTCAAATTGAGATAATTTTTACTATTGTAGACACAGATACCTCCCCTACTAGTTTGATTTCATTTAGTTCTGGTACAGTCTAGTATTATTTCCTAGCATTTCTATAAAAAAAATGTTACATGGACAAAAACCGACTTGCAGGTGCCAAATTACAATTTGATTAATGAGGTCGTCAGGTCATTTTGTACGTAGGTAGCATTACATCAAATCAACCTAGGTCATCTGAAAAGTATAATTTACATTTGCAGGAACTCAATAGTTTATGAACTATCGAAGCTGATGTCATATCTAAATTTGATGGTAAACTCTTCCTTCCTATCATTTTTAGTGGTTTCCTACTCTAATTTCCTTTAATTTGGCAAGCTTTTATATTAGCTGGTGGCAACATAATATGTTCAGTAAGCTTTATCGTGTTTTATTTATGCCCTACTGTCAACTTTAGAATTATGATTGAAAGATTTTCTGGTTTGTATTTTGAATTTGACAATTTTATTTTGTAGGTAGACTTCTCTGAATTTTCACGCTTCTATGAGTTTGTTTTCTTTGTGTGCCGTGAAAATGGTCAAAAGAATATCAGTAAGATTCCTTCTTTTGACTTGGATATAAAATTATGCTTTACTTTAATATGAATGATATCATGAAATGTGCACCAGGTAAAAAAAATCTCTTACATTCTAATAAATTTATTTTTTTCTTCTTTCATTTGAATTACAGCCGTTCATAGGGCAGTTAAAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGCAACTTTGTTGAGGTATCCTCCCCCCCATAATCAGAAAACCAGAATTTCCACCTGAAGCTATTTTCATCAACTCAGGTTTCTAGGGTCTAACATTTATGAAATGTACCTACATGTTAGACACCAATGAATGAACTTTTCTATGCTATTAGTTGTCATCCGCAGACATGCTTGTATATAAATTCATAAGTACGGACAAAGTAATATCCATTTATCTCAATTTTTGAAGTAGATGAATTTTGGGAAATTTTTGGCTATTTTTATACCAAAGATTCTAATCTATTAAATTTCTTATATCTGGGTACATGAATCCATGTTATCTTTTGGGGATCTATTCTTCAACTTGTTTCTTGATATAGAAGTACAATTTAGTGCAACTTATGTCAAGGGTTCTGATATATTTAAGTTCTATAGAACAATCAGAGACACAATATTTCTGAGGATACTTGGCAGCAAGTTTTGGCTTTCAGCCGGTGTGTACATGAAAATCTGGAAGGGTATGATCCTGAAGGTATTTGTATATATTTTTTTTTTGTTATTCTTTTAGGAGTGTACTATTAGATATTTATGTGTTAAATATATTTGAATTAAAAATGATAAGACCCTCAGGAACTACTACAATATTTTTGTAATTTTATTCGATTTTTTCTAATTAAATCGAGGCTCATGCCTCACTTCTTCTGGATAAAGAGCCTCAAGACTACATCTTAATCTTTTGAAATAGTGGGTATGATTTTTAATAAGCATAATGTTGTTTTACTGTACTGTTTAGAGTTACTCAGTTGCATTCTTCTATCAGGTGCTTGGCCTGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGTTTGTGTTTGGAGGTTTATGCAGATGCGATGAGTTTTTAATTTAATGTAGTTAAATAATTCTGGCTTATACATAATTTTCAGTGGACGGTTTAGCCTTATACTAACATATGATACGCTGATAGCTTGTAACTATTATGCAGGCGTTCTAATACAATCTCAAACCTACAATGTAATTGTGGAGACACAGAATCGCAGTCATTTGAAGATTCTCTTCCTGGTATGGATTATGTTCAACTGTCAAAGAAAAAAAAAAGTAATGTTCTATAAATGGACTTCCATGGCACGCAGTATGTGATATAAGTTGTGTTTTACTGGTTTGGATTGTGTCAGGGTTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGATGGGATGGTTTCTCTGGGTGATTCTAGAACTGGCTTCATGGAGCTGAGTCCTGTTACGAGCATCAAGAAAAGTAGATCTATGGCTGGCAAGCCTGTGAACTTGGAGATAAACTTACCTGCAAGTACTGCAGATGAGAGCATGGAAATGGTTCGACATAATGGTGCTGTGGCTTGTACCAAATCTCCTTGTGCTGTTGAAGGTTGTCTGTCAAAGGGGTTCGCTGGGCTTTTTTCGACGCGTTCGTTCTTGCGATTGGATCAAGAAAGGAAAGCATCATTCACGTAAGCACGGGTTGTTTAGCTCGTATGATTTTTACAGGTTGTAATGTGGTTATATCTTTCTTTCTTTCTTTTTCTTTTTTTTTTTTTGGTTGTGATCTAGCATTTTTAATTGGATGAGTTGATGATTTGGCACAAAACTAGAACAAAGGATTGAATGATTCTAATGAGAAAAGAAGTGAATGATTTGGCATTACGTTGTAATTGGTCTATTGCAATTCACAAATTGAATAAAGATTCTATTCTTTTCAGTCCACCTGGCTTGTAAATGAG

mRNA sequence

TTTATAGATATTCGATCTCAAAATGGCTTTTGTTGTGGGAATGAAGACTCCAGACATGTCGAAACACAAATAGCTAAAAGTTCAAAGGAGGCACTTGCAGCGCTCTTAAAATATGTGCAATCATCACTGCAGATGGGGAACTCAATAGTTTATGAACTATCGAAGCTGATGTCATATCTAAATTTGATGGTAGACTTCTCTGAATTTTCACGCTTCTATGAGTTTGTTTTCTTTGTGTGCCGTGAAAATGGTCAAAAGAATATCACCGTTCATAGGGCAGTTAAAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGCAACTTTGTTGAGAACAATCAGAGACACAATATTTCTGAGGATACTTGGCAGCAAGTTTTGGCTTTCAGCCGGTGTGTACATGAAAATCTGGAAGGGTATGATCCTGAAGGTGCTTGGCCTGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGCGTTCTAATACAATCTCAAACCTACAATGTAATTGTGGAGACACAGAATCGCAGTCATTTGAAGATTCTCTTCCTGGGTTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGATGGGATGGTTTCTCTGGGTGATTCTAGAACTGGCTTCATGGAGCTGAGTCCTGTTACGAGCATCAAGAAAAGTAGATCTATGGCTGGCAAGCCTGTGAACTTGGAGATAAACTTACCTGCAAGTACTGCAGATGAGAGCATGGAAATGGTTCGACATAATGGTGCTGTGGCTTGTACCAAATCTCCTTGTGCTGTTGAAGGTTGTCTGTCAAAGGGGTTCGCTGGGCTTTTTTCGACGCGTTCGTTCTTGCGATTGGATCAAGAAAGGAAAGCATCATTCACGTAAGCACGGGTTGTTTAGCTCGTATGATTTTTACAGGTTGTAATGTGGTTATATCTTTCTTTCTTTCTTTTTCTTTTTTTTTTTTTGGTTGTGATCTAGCATTTTTAATTGGATGAGTTGATGATTTGGCACAAAACTAGAACAAAGGATTGAATGATTCTAATGAGAAAAGAAGTGAATGATTTGGCATTACGTTGTAATTGGTCTATTGCAATTCACAAATTGAATAAAGATTCTATTCTTTTCAGTCCACCTGGCTTGTAAATGAG

Coding sequence (CDS)

TTTATAGATATTCGATCTCAAAATGGCTTTTGTTGTGGGAATGAAGACTCCAGACATGTCGAAACACAAATAGCTAAAAGTTCAAAGGAGGCACTTGCAGCGCTCTTAAAATATGTGCAATCATCACTGCAGATGGGGAACTCAATAGTTTATGAACTATCGAAGCTGATGTCATATCTAAATTTGATGGTAGACTTCTCTGAATTTTCACGCTTCTATGAGTTTGTTTTCTTTGTGTGCCGTGAAAATGGTCAAAAGAATATCACCGTTCATAGGGCAGTTAAAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGCAACTTTGTTGAGAACAATCAGAGACACAATATTTCTGAGGATACTTGGCAGCAAGTTTTGGCTTTCAGCCGGTGTGTACATGAAAATCTGGAAGGGTATGATCCTGAAGGTGCTTGGCCTGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGCGTTCTAATACAATCTCAAACCTACAATGTAATTGTGGAGACACAGAATCGCAGTCATTTGAAGATTCTCTTCCTGGGTTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGATGGGATGGTTTCTCTGGGTGATTCTAGAACTGGCTTCATGGAGCTGAGTCCTGTTACGAGCATCAAGAAAAGTAGATCTATGGCTGGCAAGCCTGTGAACTTGGAGATAAACTTACCTGCAAGTACTGCAGATGAGAGCATGGAAATGGTTCGACATAATGGTGCTGTGGCTTGTACCAAATCTCCTTGTGCTGTTGAAGGTTGTCTGTCAAAGGGGTTCGCTGGGCTTTTTTCGACGCGTTCGTTCTTGCGATTGGATCAAGAAAGGAAAGCATCATTCACGTAA

Protein sequence

FIDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSNTISNLQCNCGDTESQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQERKASFT

Homology

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BLAST of MC04g1701 vs. ExPASy Swiss-Prot
Match: Q9MBG8 (Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=AAR3 PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 8.2e-67
Identity = 148/304 (48.68%), Postives = 178/304 (58.55%), Query Frame = 0


Query: 1   FIDIRSQNGFC----CGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKL 60
           F  IRS    C    C  E+S+         SKEA+  LL  V++  Q  NSI  EL KL
Sbjct: 19  FCAIRSGQQLCNKKPCDGEESQRFNL-----SKEAITQLLYLVENKFQARNSIFDELFKL 78

Query: 61  MSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVE 120
           MS L+LMVDF+EF+ FY+FVFF+CRENGQKNIT+ RA+ AW+LVL GRFRLLN+WC+F+E
Sbjct: 79  MSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNRWCDFIE 138

Query: 121 NNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYR--RSNTISNLQC 180
            NQRHNISEDTWQQVLAFSRCVHENLEGYD EGAWPVLIDDFVEHMY     N  ++L C
Sbjct: 139 KNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMYSILGPNKDTSLFC 198

Query: 181 NCGDTESQS-----------FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMEL 240
            CGDTES+S           +     GL+  PGLKRK                       
Sbjct: 199 KCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKRK----------------------- 258

Query: 241 SPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAG 288
              TS K       +   +     +S+      +   N     +KSPC++E  LS+GFA 
Sbjct: 259 ---TSKKNDEEEEDEDEEVLETQNSSSLLNFKRIKTSNSPRCSSKSPCSIERSLSQGFAS 291

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BLAST of MC04g1701 vs. ExPASy Swiss-Prot
Match: Q9VUQ8 (DCN1-like protein OS=Drosophila melanogaster OX=7227 GN=CG7427 PE=2 SV=2)

HSP 1 Score: 103.2 bits (256), Expect = 5.2e-21
Identity = 44/96 (45.83%), Postives = 59/96 (61.46%), Query Frame = 0


Query: 65  DFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQRHNIS 124
           D  +F  FY F F   ++ GQK I +  A+  W +VL GRF+ L+ WC F+E   +  IS
Sbjct: 146 DAGKFKDFYHFTFNYAKDPGQKGIDLEMAIAYWCIVLSGRFKFLDIWCQFLEEKHKRAIS 205

Query: 125 EDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVE 161
            DTW  +L F+  + + +  YD EGAWPVLIDDFVE
Sbjct: 206 RDTWNLLLDFATNIDDRMSNYDSEGAWPVLIDDFVE 241

[Close]

BLAST of MC04g1701 vs. ExPASy Swiss-Prot
Match: Q6PH85 (DCN1-like protein 2 OS=Homo sapiens OX=9606 GN=DCUN1D2 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 5.8e-20
Identity = 46/126 (36.51%), Postives = 74/126 (58.73%), Query Frame = 0


Query: 37  KYVQSSLQMGNSIVYELSKLMSYLNL-MVDFSEFSRFYEFVFFVCRENGQKNITVHRAVK 96
           +++    ++G   + +L  L+  L   + D ++F  FY+F F   +  GQK + +  AV 
Sbjct: 121 EFLDGMTELGCDSMEKLKALLPRLEQELKDTAKFKDFYQFTFTFAKNPGQKGLDLEMAVA 180

Query: 97  AWRLVLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLI 156
            W+LVL GRF+ L+ W  F+  + + +I  DTW  +L F   + +++  YD EGAWPVLI
Sbjct: 181 YWKLVLSGRFKFLDLWNTFLMEHHKRSIPRDTWNLLLDFGNMIADDMSNYDEEGAWPVLI 240

Query: 157 DDFVEH 162
           DDFVE+
Sbjct: 241 DDFVEY 246

[Close]

BLAST of MC04g1701 vs. ExPASy Swiss-Prot
Match: Q5ZKU1 (DCN1-like protein 1 OS=Gallus gallus OX=9031 GN=DCUN1D1 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.9e-19
Identity = 41/92 (44.57%), Postives = 59/92 (64.13%), Query Frame = 0


Query: 69  FSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQRHNISEDTW 128
           F  FY+F F   +  GQK + +  A+  W LVL+GRF+ L+ W  F+  + + +I +DTW
Sbjct: 154 FKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTW 213

Query: 129 QQVLAFSRCVHENLEGYDPEGAWPVLIDDFVE 161
             +L FS  + +++  YD EGAWPVLIDDFVE
Sbjct: 214 NLLLDFSTMIADDMSNYDEEGAWPVLIDDFVE 245

[Close]

BLAST of MC04g1701 vs. ExPASy Swiss-Prot
Match: Q96GG9 (DCN1-like protein 1 OS=Homo sapiens OX=9606 GN=DCUN1D1 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.9e-19
Identity = 41/92 (44.57%), Postives = 59/92 (64.13%), Query Frame = 0


Query: 69  FSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQRHNISEDTW 128
           F  FY+F F   +  GQK + +  A+  W LVL+GRF+ L+ W  F+  + + +I +DTW
Sbjct: 154 FKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTW 213

Query: 129 QQVLAFSRCVHENLEGYDPEGAWPVLIDDFVE 161
             +L FS  + +++  YD EGAWPVLIDDFVE
Sbjct: 214 NLLLDFSTMIADDMSNYDEEGAWPVLIDDFVE 245

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BLAST of MC04g1701 vs. NCBI nr
Match: XP_022134354.1 (uncharacterized protein LOC111006635 [Momordica charantia])

HSP 1 Score: 609 bits (1570), Expect = 1.72e-219
Identity = 300/302 (99.34%), Postives = 301/302 (99.67%), Query Frame = 0


Query: 1   FIDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 60
           + DIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL
Sbjct: 22  YCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 81

Query: 61  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 120
           NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR
Sbjct: 82  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 141

Query: 121 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSNTISNLQCNCGDTE 180
           HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSNTISNLQCNCGDTE
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSNTISNLQCNCGDTE 201

Query: 181 SQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAGKPV 240
           SQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAGKPV
Sbjct: 202 SQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAGKPV 261

Query: 241 NLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQERKAS 300
           NLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQERKAS
Sbjct: 262 NLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQERKAS 321

Query: 301 FT 302
           FT
Sbjct: 322 FT 323

[Close]

BLAST of MC04g1701 vs. NCBI nr
Match: XP_038890437.1 (uncharacterized protein LOC120080000 isoform X1 [Benincasa hispida])

HSP 1 Score: 503 bits (1294), Expect = 2.17e-177
Identity = 256/306 (83.66%), Postives = 273/306 (89.22%), Query Frame = 0


Query: 1   FIDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 60
           + DIRSQNGFC GNE+S  VETQ+AKS K+ALA LL YVQSSLQMGNSIVYELSKLMSYL
Sbjct: 22  YCDIRSQNGFCHGNEESGQVETQMAKSPKQALAELLIYVQSSLQMGNSIVYELSKLMSYL 81

Query: 61  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 120
           N MVDFSEFSRFYEFVFFVCRENGQKNITV  AV+AWRLVL+GRFRLLNQWC+FVENNQR
Sbjct: 82  NFMVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRLVLNGRFRLLNQWCDFVENNQR 141

Query: 121 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS--NTISNLQCNCGD 180
           HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYR+S  N +S   CNCGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNKVSKFCCNCGD 201

Query: 181 TESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMA 240
           +ESQS  FEDSL GLKFFPGLKRKLPEDMQMDG++S  D RTG MELSPV S+KKSR MA
Sbjct: 202 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIMSPSDPRTGSMELSPVLSVKKSRFMA 261

Query: 241 GKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQE 300
            +PVNLEINLP+ TADE+ EMVRHN AV  +KSPCAVEGCLSKGFAGLFSTR FL LDQE
Sbjct: 262 CRPVNLEINLPSCTADENTEMVRHNSAVG-SKSPCAVEGCLSKGFAGLFSTRLFLGLDQE 321

Query: 301 RKASFT 302
           RKASFT
Sbjct: 322 RKASFT 326

[Close]

BLAST of MC04g1701 vs. NCBI nr
Match: XP_038890438.1 (uncharacterized protein LOC120080000 isoform X2 [Benincasa hispida])

HSP 1 Score: 499 bits (1286), Expect = 1.87e-176
Identity = 255/304 (83.88%), Postives = 272/304 (89.47%), Query Frame = 0


Query: 3   DIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYLNL 62
           +IRSQNGFC GNE+S  VETQ+AKS K+ALA LL YVQSSLQMGNSIVYELSKLMSYLN 
Sbjct: 6   NIRSQNGFCHGNEESGQVETQMAKSPKQALAELLIYVQSSLQMGNSIVYELSKLMSYLNF 65

Query: 63  MVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQRHN 122
           MVDFSEFSRFYEFVFFVCRENGQKNITV  AV+AWRLVL+GRFRLLNQWC+FVENNQRHN
Sbjct: 66  MVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRLVLNGRFRLLNQWCDFVENNQRHN 125

Query: 123 ISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS--NTISNLQCNCGDTE 182
           ISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYR+S  N +S   CNCGD+E
Sbjct: 126 ISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNKVSKFCCNCGDSE 185

Query: 183 SQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAGK 242
           SQS  FEDSL GLKFFPGLKRKLPEDMQMDG++S  D RTG MELSPV S+KKSR MA +
Sbjct: 186 SQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIMSPSDPRTGSMELSPVLSVKKSRFMACR 245

Query: 243 PVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQERK 302
           PVNLEINLP+ TADE+ EMVRHN AV  +KSPCAVEGCLSKGFAGLFSTR FL LDQERK
Sbjct: 246 PVNLEINLPSCTADENTEMVRHNSAVG-SKSPCAVEGCLSKGFAGLFSTRLFLGLDQERK 305

[Close]

BLAST of MC04g1701 vs. NCBI nr
Match: XP_004148700.1 (uncharacterized protein LOC101214663 [Cucumis sativus] >KGN52464.1 hypothetical protein Csa_007882 [Cucumis sativus])

HSP 1 Score: 488 bits (1255), Expect = 1.75e-171
Identity = 249/305 (81.64%), Postives = 267/305 (87.54%), Query Frame = 0


Query: 1   FIDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 60
           + DIRSQNG+  GNEDS + ETQ+ KSSK+ALA LL YVQSSLQMGNS+VYELSKLMSYL
Sbjct: 22  YCDIRSQNGYH-GNEDSGNAETQMCKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 81

Query: 61  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 120
           N MVDFSEFSRFYEFVFFVCRENGQKNITV  AV+AWRLVLDGRFRLLNQWC+FVENNQR
Sbjct: 82  NFMVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 121 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS--NTISNLQCNCGD 180
           HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS  N +S L CNCGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKVSKLSCNCGD 201

Query: 181 TESQS-FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAG 240
           +E    FEDSL GLKFFPGLKRKLPEDMQMDG+VS  D RT  ME +PVTS+KKSR MA 
Sbjct: 202 SEESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSDPRTSAMEFTPVTSVKKSRFMAC 261

Query: 241 KPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQER 300
           +PVNLEIN P+ TA E++EMVRHN  V  +KSPCAVEGCLSKGFAGLFSTR FL LDQER
Sbjct: 262 RPVNLEINSPSCTAAENIEMVRHNSTVG-SKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 321

Query: 301 KASFT 302
           KASFT
Sbjct: 322 KASFT 324

[Close]

BLAST of MC04g1701 vs. NCBI nr
Match: XP_008459316.1 (PREDICTED: LOW QUALITY PROTEIN: defective in cullin neddylation protein 1 [Cucumis melo])

HSP 1 Score: 488 bits (1255), Expect = 1.82e-171
Identity = 249/306 (81.37%), Postives = 271/306 (88.56%), Query Frame = 0


Query: 1   FIDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 60
           + DIRSQNG+  GNEDS + ETQ++KSSK+ALA LL YVQSSLQMGNS+VYELSKLMSYL
Sbjct: 22  YCDIRSQNGYH-GNEDSGYAETQMSKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 81

Query: 61  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 120
           N MVDFSEFSRFYEF FFVCRENGQKNITV  AV+AWRLVLDGRFRLLNQWC+FVENNQR
Sbjct: 82  NFMVDFSEFSRFYEFXFFVCRENGQKNITVSMAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 121 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS--NTISNLQCNCGD 180
           HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS  N +S L C CGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKVSKLCCTCGD 201

Query: 181 TESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMA 240
           +ES+S  FEDSL GLKFFPGLKRKLPEDMQMDG+VS  D RTG ME +PVTS+KKSR MA
Sbjct: 202 SESESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSDPRTGAMEFTPVTSVKKSRFMA 261

Query: 241 GKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQE 300
            +PVNLEINLP+ TA E++E+VRH+  V  +KSPCAVEGCLSKGFAGLFSTR FL LDQE
Sbjct: 262 CRPVNLEINLPSCTAAENIEVVRHSSTVG-SKSPCAVEGCLSKGFAGLFSTRLFLGLDQE 321

Query: 301 RKASFT 302
           RKASFT
Sbjct: 322 RKASFT 325

[Close]

BLAST of MC04g1701 vs. ExPASy TrEMBL
Match: A0A6J1BYJ7 (Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111006635 PE=4 SV=1)

HSP 1 Score: 609 bits (1570), Expect = 8.34e-220
Identity = 300/302 (99.34%), Postives = 301/302 (99.67%), Query Frame = 0


Query: 1   FIDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 60
           + DIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL
Sbjct: 22  YCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 81

Query: 61  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 120
           NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR
Sbjct: 82  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 141

Query: 121 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSNTISNLQCNCGDTE 180
           HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSNTISNLQCNCGDTE
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSNTISNLQCNCGDTE 201

Query: 181 SQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAGKPV 240
           SQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAGKPV
Sbjct: 202 SQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAGKPV 261

Query: 241 NLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQERKAS 300
           NLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQERKAS
Sbjct: 262 NLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQERKAS 321

Query: 301 FT 302
           FT
Sbjct: 322 FT 323

[Close]

BLAST of MC04g1701 vs. ExPASy TrEMBL
Match: A0A0A0KU82 (Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_5G636560 PE=4 SV=1)

HSP 1 Score: 488 bits (1255), Expect = 8.49e-172
Identity = 249/305 (81.64%), Postives = 267/305 (87.54%), Query Frame = 0


Query: 1   FIDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 60
           + DIRSQNG+  GNEDS + ETQ+ KSSK+ALA LL YVQSSLQMGNS+VYELSKLMSYL
Sbjct: 22  YCDIRSQNGYH-GNEDSGNAETQMCKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 81

Query: 61  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 120
           N MVDFSEFSRFYEFVFFVCRENGQKNITV  AV+AWRLVLDGRFRLLNQWC+FVENNQR
Sbjct: 82  NFMVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 121 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS--NTISNLQCNCGD 180
           HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS  N +S L CNCGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKVSKLSCNCGD 201

Query: 181 TESQS-FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAG 240
           +E    FEDSL GLKFFPGLKRKLPEDMQMDG+VS  D RT  ME +PVTS+KKSR MA 
Sbjct: 202 SEESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSDPRTSAMEFTPVTSVKKSRFMAC 261

Query: 241 KPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQER 300
           +PVNLEIN P+ TA E++EMVRHN  V  +KSPCAVEGCLSKGFAGLFSTR FL LDQER
Sbjct: 262 RPVNLEINSPSCTAAENIEMVRHNSTVG-SKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 321

Query: 301 KASFT 302
           KASFT
Sbjct: 322 KASFT 324

[Close]

BLAST of MC04g1701 vs. ExPASy TrEMBL
Match: A0A1S3CB35 (Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103498486 PE=4 SV=1)

HSP 1 Score: 488 bits (1255), Expect = 8.81e-172
Identity = 249/306 (81.37%), Postives = 271/306 (88.56%), Query Frame = 0


Query: 1   FIDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 60
           + DIRSQNG+  GNEDS + ETQ++KSSK+ALA LL YVQSSLQMGNS+VYELSKLMSYL
Sbjct: 22  YCDIRSQNGYH-GNEDSGYAETQMSKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 81

Query: 61  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 120
           N MVDFSEFSRFYEF FFVCRENGQKNITV  AV+AWRLVLDGRFRLLNQWC+FVENNQR
Sbjct: 82  NFMVDFSEFSRFYEFXFFVCRENGQKNITVSMAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 121 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS--NTISNLQCNCGD 180
           HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS  N +S L C CGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKVSKLCCTCGD 201

Query: 181 TESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMA 240
           +ES+S  FEDSL GLKFFPGLKRKLPEDMQMDG+VS  D RTG ME +PVTS+KKSR MA
Sbjct: 202 SESESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSDPRTGAMEFTPVTSVKKSRFMA 261

Query: 241 GKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQE 300
            +PVNLEINLP+ TA E++E+VRH+  V  +KSPCAVEGCLSKGFAGLFSTR FL LDQE
Sbjct: 262 CRPVNLEINLPSCTAAENIEVVRHSSTVG-SKSPCAVEGCLSKGFAGLFSTRLFLGLDQE 321

Query: 301 RKASFT 302
           RKASFT
Sbjct: 322 RKASFT 325

[Close]

BLAST of MC04g1701 vs. ExPASy TrEMBL
Match: A0A6J1FFS5 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111445042 PE=4 SV=1)

HSP 1 Score: 478 bits (1230), Expect = 5.84e-168
Identity = 245/305 (80.33%), Postives = 268/305 (87.87%), Query Frame = 0


Query: 1   FIDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 60
           + DIRSQNG   GNEDS HVE+QIAKSSK+ALA L  YV+SSLQMGNSIV EL+K+MS+ 
Sbjct: 22  YCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDELAKVMSHP 81

Query: 61  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 120
           NLMVDFSEFSRFYEFVFFV RENGQKNITV RAV+AWRLVLDGRFRLLNQWC+FVENNQR
Sbjct: 82  NLMVDFSEFSRFYEFVFFVSRENGQKNITVGRAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 121 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS--NTISNLQCNCGD 180
           HNISEDTW QVLAFS CVHENLEGYDPEGAWPVLIDDFVEHMYRRS  N IS L C CGD
Sbjct: 142 HNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKISKLYCTCGD 201

Query: 181 TESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMA 240
           +ESQS  FEDSLPGLK FPGLKRKLPEDMQ+D ++SL D RTG MEL PVTS+KK+R M 
Sbjct: 202 SESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDIMSLSDPRTGSMELIPVTSLKKTRFMG 261

Query: 241 GKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQE 300
            +PVNLE+NLP+ TADE+++MVRHNGAV C KSPCA+EG LSKGFAGLFSTRS L LD+E
Sbjct: 262 CRPVNLEVNLPSCTADENIDMVRHNGAVGC-KSPCAIEGFLSKGFAGLFSTRSILGLDRE 321

[Close]

BLAST of MC04g1701 vs. ExPASy TrEMBL
Match: A0A6J1GSP1 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111457165 PE=4 SV=1)

HSP 1 Score: 476 bits (1225), Expect = 3.14e-167
Identity = 246/312 (78.85%), Postives = 266/312 (85.26%), Query Frame = 0


Query: 1   FIDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 60
           +  IRSQNG C GN    HVETQ+AKSSK+ALA LL YVQSSL  GNSI++ELSKLMSYL
Sbjct: 13  YCGIRSQNGLCHGNGGFGHVETQMAKSSKQALAELLIYVQSSLHTGNSILHELSKLMSYL 72

Query: 61  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 120
           NLMVDFSEFSRFYEFVFFVCRENGQKNITV RA+KAWRLVLDGRFRLLNQWC+FVENNQR
Sbjct: 73  NLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLLNQWCDFVENNQR 132

Query: 121 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRS--NTISNLQCNCGD 180
           HNISEDTW QVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYR S  N IS L+C+CG 
Sbjct: 133 HNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGSNKISKLRCSCGG 192

Query: 181 TESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMA 240
           +ESQS   ED  PGLK  PGLKRKLPED+QMDGMVS  D  TG MELSPVTSIKKSR MA
Sbjct: 193 SESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMELSPVTSIKKSRFMA 252

Query: 241 GKPVNLEINLP------ASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSF 300
            KPVNLEIN P      A+ AD + E+VR+NGA+ C+K+PCAVEGCLS+GFAGLFSTRSF
Sbjct: 253 CKPVNLEINGPSCNAAAAAAADGNTEIVRNNGAMGCSKAPCAVEGCLSQGFAGLFSTRSF 312

Query: 301 LRLDQERKASFT 302
           L LDQERK+SFT
Sbjct: 313 LGLDQERKSSFT 324

[Close]

BLAST of MC04g1701 vs. TAIR 10
Match: AT3G28970.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 255.4 bits (651), Expect = 5.8e-68
Identity = 148/304 (48.68%), Postives = 178/304 (58.55%), Query Frame = 0


Query: 1   FIDIRSQNGFC----CGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKL 60
           F  IRS    C    C  E+S+         SKEA+  LL  V++  Q  NSI  EL KL
Sbjct: 19  FCAIRSGQQLCNKKPCDGEESQRFNL-----SKEAITQLLYLVENKFQARNSIFDELFKL 78

Query: 61  MSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVE 120
           MS L+LMVDF+EF+ FY+FVFF+CRENGQKNIT+ RA+ AW+LVL GRFRLLN+WC+F+E
Sbjct: 79  MSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNRWCDFIE 138

Query: 121 NNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYR--RSNTISNLQC 180
            NQRHNISEDTWQQVLAFSRCVHENLEGYD EGAWPVLIDDFVEHMY     N  ++L C
Sbjct: 139 KNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMYSILGPNKDTSLFC 198

Query: 181 NCGDTESQS-----------FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMEL 240
            CGDTES+S           +     GL+  PGLKRK                       
Sbjct: 199 KCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKRK----------------------- 258

Query: 241 SPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAG 288
              TS K       +   +     +S+      +   N     +KSPC++E  LS+GFA 
Sbjct: 259 ---TSKKNDEEEEDEDEEVLETQNSSSLLNFKRIKTSNSPRCSSKSPCSIERSLSQGFAS 291

[Close]

BLAST of MC04g1701 vs. TAIR 10
Match: AT3G12760.1 (CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IPR014764), Protein of unknown function DUF298 (InterPro:IPR005176), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: Domain of unknown function (DUF298) (TAIR:AT1G15860.2); Has 857 Blast hits to 855 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 482; Fungi - 154; Plants - 139; Viruses - 0; Other Eukaryotes - 82 (source: NCBI BLink). )

HSP 1 Score: 94.7 bits (234), Expect = 1.3e-19
Identity = 45/132 (34.09%), Postives = 78/132 (59.09%), Query Frame = 0


Query: 37  KYVQSSLQMGNSIVYELSKLMSYL-NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVK 96
           +++     +G   + +L + + ++ + + D  +F   Y F F   +E GQK++ +  A+ 
Sbjct: 115 EFISGLQALGVDSIGKLQEKLPFMRSELKDEQKFHEIYNFAFGWAKEKGQKSLALDTAIG 174

Query: 97  AWRLVLDGR-FRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVL 156
            W+L+   R + L+  WC+F+++     IS+DTW Q+L FSR V   L  YD EGAWP L
Sbjct: 175 MWQLLFAEREWPLVTHWCDFLQDRHNKAISKDTWAQLLEFSRMVDPVLSNYDAEGAWPYL 234

Query: 157 IDDFVEHMYRRS 167
           ID+FVE++Y ++
Sbjct: 235 IDEFVEYLYDKN 246

[Close]

BLAST of MC04g1701 vs. TAIR 10
Match: AT1G15860.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 49.3 bits (116), Expect = 6.3e-06
Identity = 32/100 (32.00%), Postives = 49/100 (49.00%), Query Frame = 0


Query: 67  SEFSRFYEFVFFVC-RENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVE----NNQRH 126
           S F+ FY + F  C  E  QK+I +    +   +V+   FR   Q   FVE     N   
Sbjct: 123 SNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRA--QVDYFVEYLKIQNDYK 182

Query: 127 NISEDTWQQVLAF-SRCVHENLEGYDPEGAWPVLIDDFVE 161
            I+ D W  +  F +     ++  Y+PE AWP+++D+FVE
Sbjct: 183 VINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVE 220

[Close]

BLAST of MC04g1701 vs. TAIR 10
Match: AT1G15860.3 (Domain of unknown function (DUF298) )

HSP 1 Score: 47.4 bits (111), Expect = 2.4e-05
Identity = 30/107 (28.04%), Postives = 49/107 (45.79%), Query Frame = 0


Query: 67  SEFSRFYEFVFFVC-RENGQKNITVHRAVKAWRLVLDGRFR--------LLNQWC---NF 126
           S F+ FY + F  C  E  QK+I +    +   +V+   FR         L  W    + 
Sbjct: 123 SNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKVWITQKSH 182

Query: 127 VENNQRHNISEDTWQQVLAF-SRCVHENLEGYDPEGAWPVLIDDFVE 161
           +  N    I+ D W  +  F +     ++  Y+PE AWP+++D+FVE
Sbjct: 183 IIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVE 229

The following BLAST results are available for this feature:

BLAST of MC04g1701 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG8	8.2e-67	48.68	Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9VUQ8	5.2e-21	45.83	DCN1-like protein OS=Drosophila melanogaster OX=7227 GN=CG7427 PE=2 SV=2	[more]
Q6PH85	5.8e-20	36.51	DCN1-like protein 2 OS=Homo sapiens OX=9606 GN=DCUN1D2 PE=1 SV=1	[more]
Q5ZKU1	2.9e-19	44.57	DCN1-like protein 1 OS=Gallus gallus OX=9031 GN=DCUN1D1 PE=2 SV=1	[more]
Q96GG9	2.9e-19	44.57	DCN1-like protein 1 OS=Homo sapiens OX=9606 GN=DCUN1D1 PE=1 SV=1	[more]

BLAST of MC04g1701 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134354.1	1.72e-219	99.34	uncharacterized protein LOC111006635 [Momordica charantia]	[more]
XP_038890437.1	2.17e-177	83.66	uncharacterized protein LOC120080000 isoform X1 [Benincasa hispida]	[more]
XP_038890438.1	1.87e-176	83.88	uncharacterized protein LOC120080000 isoform X2 [Benincasa hispida]	[more]
XP_004148700.1	1.75e-171	81.64	uncharacterized protein LOC101214663 [Cucumis sativus] >KGN52464.1 hypothetical ...	[more]
XP_008459316.1	1.82e-171	81.37	PREDICTED: LOW QUALITY PROTEIN: defective in cullin neddylation protein 1 [Cucum...	[more]

BLAST of MC04g1701 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BYJ7	8.34e-220	99.34	Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A0A0KU82	8.49e-172	81.64	Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_5G6365...	[more]
A0A1S3CB35	8.81e-172	81.37	Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103498486 ...	[more]
A0A6J1FFS5	5.84e-168	80.33	Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1GSP1	3.14e-167	78.85	Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of MC04g1701 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G28970.1	5.8e-68	48.68	Domain of unknown function (DUF298)	[more]
AT3G12760.1	1.3e-19	34.09	CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IP...	[more]
AT1G15860.1	6.3e-06	32.00	Domain of unknown function (DUF298)	[more]
AT1G15860.3	2.4e-05	28.04	Domain of unknown function (DUF298)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005176	Potentiating neddylation domain	PFAM	PF03556	Cullin_binding	coord: 64..161 e-value: 1.1E-29 score: 103.3
IPR005176	Potentiating neddylation domain	PROSITE	PS51229	DCUN1	coord: 1..163 score: 26.404251
IPR042460	DCN1-like, PONY binding domain	GENE3D	1.10.238.200	Cullin, PONY binding domain	coord: 65..172 e-value: 8.9E-36 score: 124.3
None	No IPR available	PANTHER	PTHR12281:SF31	DEFECTIVE IN CULLIN NEDDYLATION PROTEIN	coord: 1..212
IPR014764	Defective-in-cullin neddylation protein	PANTHER	PTHR12281	RP42 RELATED	coord: 1..212

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g1701.1	MC04g1701.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051443	positive regulation of ubiquitin-protein transferase activity
biological_process	GO:0045116	protein neddylation
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0097602	cullin family protein binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0032182	ubiquitin-like protein binding