MC04g1664 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g1664
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	metalloendoproteinase 2-MMP-like
Location	MC04: 24105514 .. 24105714 (-)
RNA-Seq Expression	MC04g1664
Synteny	MC04g1664

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATATCAACGTGGAGCAGTGCTGGACGGTGGGGGCTCGCCCAAATTGTCAAGATGTGATGTCGGTGGCAGTGCATGAGATTGGGCATCTTCTTGGGCTCGGCCACAGCGGCGCCGAGGAAGCCATCATGTGGCCATACATACGCCCCAACGCTGTCAAGGGCTTGTTTCAAGACGATGTTGCTGGAATCAAGGCTTTGTAT

mRNA sequence

AATATCAACGTGGAGCAGTGCTGGACGGTGGGGGCTCGCCCAAATTGTCAAGATGTGATGTCGGTGGCAGTGCATGAGATTGGGCATCTTCTTGGGCTCGGCCACAGCGGCGCCGAGGAAGCCATCATGTGGCCATACATACGCCCCAACGCTGTCAAGGGCTTGTTTCAAGACGATGTTGCTGGAATCAAGGCTTTGTAT

Coding sequence (CDS)

AATATCAACGTGGAGCAGTGCTGGACGGTGGGGGCTCGCCCAAATTGTCAAGATGTGATGTCGGTGGCAGTGCATGAGATTGGGCATCTTCTTGGGCTCGGCCACAGCGGCGCCGAGGAAGCCATCATGTGGCCATACATACGCCCCAACGCTGTCAAGGGCTTGTTTCAAGACGATGTTGCTGGAATCAAGGCTTTGTAT

Protein sequence

NINVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDVAGIKALY

Homology

[Close]

BLAST of MC04g1664 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.2e-10
Identity = 34/73 (46.58%), Postives = 44/73 (60.27%), Query Frame = 0


Query: 1   NINVEQCWTV-----GARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVK-G 60
           +++  + W V     G+     D+ SVA HEIGHLLGLGHS  E A+M+P +RP   K  
Sbjct: 246 HLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVD 305

Query: 61  LFQDDVAGIKALY 68
           L  DDVAG+  LY
Sbjct: 306 LTVDDVAGVLKLY 318

[Close]

BLAST of MC04g1664 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.0e-09
Identity = 28/51 (54.90%), Postives = 39/51 (76.47%), Query Frame = 0


Query: 18  DVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVK-GLFQDDVAGIKALY 68
           D+ SVAVHEIGH+LGLGHS  ++A M+P ++P + K  L  DDV G+++LY
Sbjct: 245 DLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

[Close]

BLAST of MC04g1664 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 1.3e-09
Identity = 32/51 (62.75%), Postives = 36/51 (70.59%), Query Frame = 0


Query: 18  DVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVK-GLFQDDVAGIKALY 68
           D+ SVAVHEIGHLLGLGHS    AIM+P I P   K  L QDD+ GI+ LY
Sbjct: 251 DLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]

BLAST of MC04g1664 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.3e-09
Identity = 32/51 (62.75%), Postives = 36/51 (70.59%), Query Frame = 0


Query: 18  DVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVK-GLFQDDVAGIKALY 68
           D+ SVAVHEIGHLLGLGHS  EE+IM+P I     K  L  DDV GI+ LY
Sbjct: 273 DLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of MC04g1664 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.0e-09
Identity = 31/51 (60.78%), Postives = 36/51 (70.59%), Query Frame = 0


Query: 18  DVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVK-GLFQDDVAGIKALY 68
           D+ SVAVHEIGHLLGLGHS  E +IM+P IR    K  L  DDV G++ LY
Sbjct: 278 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of MC04g1664 vs. NCBI nr
Match: XP_022133610.1 (metalloendoproteinase 1-like [Momordica charantia])

HSP 1 Score: 137 bits (344), Expect = 1.14e-37
Identity = 63/67 (94.03%), Postives = 65/67 (97.01%), Query Frame = 0


Query: 1   NINVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDV 60
           + NVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWP IRPNAVKGLFQD+V
Sbjct: 238 HFNVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNV 297

Query: 61  AGIKALY 67
           AGIKALY
Sbjct: 298 AGIKALY 304

[Close]

BLAST of MC04g1664 vs. NCBI nr
Match: XP_022133608.1 (matrix metalloproteinase-9-like [Momordica charantia])

HSP 1 Score: 93.6 bits (231), Expect = 3.84e-21
Identity = 42/67 (62.69%), Postives = 53/67 (79.10%), Query Frame = 0


Query: 1   NINVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDV 60
           ++NV+Q W+VGA+    DV SVA+HEIGHLLGL HS  +EAIMWP I  N VKGL+ DD+
Sbjct: 205 HLNVDQKWSVGAQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDI 264

Query: 61  AGIKALY 67
           AG++ALY
Sbjct: 265 AGLEALY 271

[Close]

BLAST of MC04g1664 vs. NCBI nr
Match: XP_021980473.1 (metalloendoproteinase 2-MMP [Helianthus annuus] >KAF5792118.1 putative matrilysin [Helianthus annuus])

HSP 1 Score: 88.6 bits (218), Expect = 3.50e-19
Identity = 41/65 (63.08%), Postives = 48/65 (73.85%), Query Frame = 0


Query: 3   NVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDVAG 62
           N +Q W++GAR N  DV +VA+HEIGHLLGLGHS  + AIMW  I   AVKGL  DD+ G
Sbjct: 218 NADQSWSIGARQNSFDVETVALHEIGHLLGLGHSQFQNAIMWSAISSGAVKGLASDDIQG 277

Query: 63  IKALY 67
           IKALY
Sbjct: 278 IKALY 282

[Close]

BLAST of MC04g1664 vs. NCBI nr
Match: XP_021980471.1 (metalloendoproteinase 2-MMP [Helianthus annuus] >KAF5792117.1 putative matrilysin [Helianthus annuus])

HSP 1 Score: 88.6 bits (218), Expect = 3.50e-19
Identity = 41/65 (63.08%), Postives = 48/65 (73.85%), Query Frame = 0


Query: 3   NVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDVAG 62
           N +Q W++GAR N  DV +VA+HEIGHLLGLGHS  + AIMW  I   AVKGL  DD+ G
Sbjct: 218 NADQSWSIGARQNSFDVETVALHEIGHLLGLGHSQFQNAIMWSAISSGAVKGLASDDIQG 277

Query: 63  IKALY 67
           IKALY
Sbjct: 278 IKALY 282

[Close]

BLAST of MC04g1664 vs. NCBI nr
Match: XP_030973479.1 (metalloendoproteinase 2-MMP-like [Quercus lobata])

HSP 1 Score: 88.6 bits (218), Expect = 5.04e-19
Identity = 39/65 (60.00%), Postives = 49/65 (75.38%), Query Frame = 0


Query: 3   NVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDVAG 62
           + ++ W+VGA PN  D+ +VA+HEIGHLLGL HS  E AIMWP IRP   +GL +DD+ G
Sbjct: 247 DADEQWSVGAAPNAFDLETVALHEIGHLLGLDHSSVEGAIMWPNIRPGVTQGLHRDDIDG 306

Query: 63  IKALY 67
           IKALY
Sbjct: 307 IKALY 311

[Close]

BLAST of MC04g1664 vs. ExPASy TrEMBL
Match: A0A6J1BVR3 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3 SV=1)

HSP 1 Score: 137 bits (344), Expect = 5.51e-38
Identity = 63/67 (94.03%), Postives = 65/67 (97.01%), Query Frame = 0


Query: 1   NINVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDV 60
           + NVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWP IRPNAVKGLFQD+V
Sbjct: 238 HFNVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNV 297

Query: 61  AGIKALY 67
           AGIKALY
Sbjct: 298 AGIKALY 304

[Close]

BLAST of MC04g1664 vs. ExPASy TrEMBL
Match: A0A6J1BZL4 (matrix metalloproteinase-9-like OS=Momordica charantia OX=3673 GN=LOC111006147 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.86e-21
Identity = 42/67 (62.69%), Postives = 53/67 (79.10%), Query Frame = 0


Query: 1   NINVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDV 60
           ++NV+Q W+VGA+    DV SVA+HEIGHLLGL HS  +EAIMWP I  N VKGL+ DD+
Sbjct: 205 HLNVDQKWSVGAQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDI 264

Query: 61  AGIKALY 67
           AG++ALY
Sbjct: 265 AGLEALY 271

[Close]

BLAST of MC04g1664 vs. ExPASy TrEMBL
Match: A0A251TYU0 (Putative peptidase M10A, Metallopeptidase, catalytic domain protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr09g0265401 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.69e-19
Identity = 41/65 (63.08%), Postives = 48/65 (73.85%), Query Frame = 0


Query: 3   NVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDVAG 62
           N +Q W++GAR N  DV +VA+HEIGHLLGLGHS  + AIMW  I   AVKGL  DD+ G
Sbjct: 218 NADQSWSIGARQNSFDVETVALHEIGHLLGLGHSQFQNAIMWSAISSGAVKGLASDDIQG 277

Query: 63  IKALY 67
           IKALY
Sbjct: 278 IKALY 282

[Close]

BLAST of MC04g1664 vs. ExPASy TrEMBL
Match: A0A251TYA8 (Putative peptidase M10A, Metallopeptidase, catalytic domain protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr09g0265411 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.69e-19
Identity = 41/65 (63.08%), Postives = 48/65 (73.85%), Query Frame = 0


Query: 3   NVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDVAG 62
           N +Q W++GAR N  DV +VA+HEIGHLLGLGHS  + AIMW  I   AVKGL  DD+ G
Sbjct: 218 NADQSWSIGARQNSFDVETVALHEIGHLLGLGHSQFQNAIMWSAISSGAVKGLASDDIQG 277

Query: 63  IKALY 67
           IKALY
Sbjct: 278 IKALY 282

[Close]

BLAST of MC04g1664 vs. ExPASy TrEMBL
Match: A0A7N2LXS1 (ZnMc domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 2.44e-19
Identity = 39/65 (60.00%), Postives = 49/65 (75.38%), Query Frame = 0


Query: 3   NVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVKGLFQDDVAG 62
           + ++ W+VGA PN  D+ +VA+HEIGHLLGL HS  E AIMWP IRP   +GL +DD+ G
Sbjct: 247 DADEQWSVGAAPNAFDLETVALHEIGHLLGLDHSSVEGAIMWPNIRPGVTQGLHRDDIDG 306

Query: 63  IKALY 67
           IKALY
Sbjct: 307 IKALY 311

[Close]

BLAST of MC04g1664 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 66.6 bits (161), Expect = 8.5e-12
Identity = 34/73 (46.58%), Postives = 44/73 (60.27%), Query Frame = 0


Query: 1   NINVEQCWTV-----GARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVK-G 60
           +++  + W V     G+     D+ SVA HEIGHLLGLGHS  E A+M+P +RP   K  
Sbjct: 246 HLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVD 305

Query: 61  LFQDDVAGIKALY 68
           L  DDVAG+  LY
Sbjct: 306 LTVDDVAGVLKLY 318

[Close]

BLAST of MC04g1664 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 63.5 bits (153), Expect = 7.2e-11
Identity = 28/51 (54.90%), Postives = 39/51 (76.47%), Query Frame = 0


Query: 18  DVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVK-GLFQDDVAGIKALY 68
           D+ SVAVHEIGH+LGLGHS  ++A M+P ++P + K  L  DDV G+++LY
Sbjct: 245 DLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

[Close]

BLAST of MC04g1664 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 62.4 bits (150), Expect = 1.6e-10
Identity = 32/51 (62.75%), Postives = 36/51 (70.59%), Query Frame = 0


Query: 18  DVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVK-GLFQDDVAGIKALY 68
           D+ SVAVHEIGHLLGLGHS  EE+IM+P I     K  L  DDV GI+ LY
Sbjct: 273 DLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of MC04g1664 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 61.2 bits (147), Expect = 3.6e-10
Identity = 31/51 (60.78%), Postives = 36/51 (70.59%), Query Frame = 0


Query: 18  DVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVK-GLFQDDVAGIKALY 68
           D+ SVAVHEIGHLLGLGHS  E +IM+P IR    K  L  DDV G++ LY
Sbjct: 278 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of MC04g1664 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 60.8 bits (146), Expect = 4.7e-10
Identity = 31/51 (60.78%), Postives = 37/51 (72.55%), Query Frame = 0


Query: 18  DVMSVAVHEIGHLLGLGHSGAEEAIMWPYIRPNAVK-GLFQDDVAGIKALY 68
           D+ SVAVHEIGHLLGLGHS  E+AIM+P I     K  L +DD+ GI+ LY
Sbjct: 263 DLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

The following BLAST results are available for this feature:

BLAST of MC04g1664 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23507	1.2e-10	46.58	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]
Q8GWW6	1.0e-09	54.90	Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1	[more]
P29136	1.3e-09	62.75	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
O04529	2.3e-09	62.75	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q5XF51	5.0e-09	60.78	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]

BLAST of MC04g1664 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133610.1	1.14e-37	94.03	metalloendoproteinase 1-like [Momordica charantia]	[more]
XP_022133608.1	3.84e-21	62.69	matrix metalloproteinase-9-like [Momordica charantia]	[more]
XP_021980473.1	3.50e-19	63.08	metalloendoproteinase 2-MMP [Helianthus annuus] >KAF5792118.1 putative matrilysi...	[more]
XP_021980471.1	3.50e-19	63.08	metalloendoproteinase 2-MMP [Helianthus annuus] >KAF5792117.1 putative matrilysi...	[more]
XP_030973479.1	5.04e-19	60.00	metalloendoproteinase 2-MMP-like [Quercus lobata]	[more]

BLAST of MC04g1664 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVR3	5.51e-38	94.03	metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3...	[more]
A0A6J1BZL4	1.86e-21	62.69	matrix metalloproteinase-9-like OS=Momordica charantia OX=3673 GN=LOC111006147 P...	[more]
A0A251TYU0	1.69e-19	63.08	Putative peptidase M10A, Metallopeptidase, catalytic domain protein OS=Helianthu...	[more]
A0A251TYA8	1.69e-19	63.08	Putative peptidase M10A, Metallopeptidase, catalytic domain protein OS=Helianthu...	[more]
A0A7N2LXS1	2.44e-19	60.00	ZnMc domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]

BLAST of MC04g1664 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16640.1	8.5e-12	46.58	Matrixin family protein	[more]
AT2G45040.1	7.2e-11	54.90	Matrixin family protein	[more]
AT1G70170.1	1.6e-10	62.75	matrix metalloproteinase	[more]
AT1G24140.1	3.6e-10	60.78	Matrixin family protein	[more]
AT1G59970.1	4.7e-10	60.78	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 22..47 score: 56.8 coord: 55..67 score: 53.31
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 5..67 e-value: 5.6E-17 score: 62.0
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 1..67 e-value: 1.3E-19 score: 72.5
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 5..67
None	No IPR available	PANTHER	PTHR10201:SF272	METALLOENDOPROTEINASE 2-MMP-LIKE	coord: 5..67
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 4..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g1664.1	MC04g1664.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity