MC04g1657 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g1657 vs. ExPASy Swiss-Prot
Match: Q9C923 (Nuclear/nucleolar GTPase 2 OS=Arabidopsis thaliana OX=3702 GN=NUG2 PE=1 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 5.8e-236
Identity = 411/550 (74.73%), Postives = 476/550 (86.55%), Query Frame = 0

Query: 4   KKEKKVNVSGKPKHSLDVNRSDANK---NSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 63
           KKEKK NVSGKPKHSLD NR+D  K    +RS +TV RLKMY TRPKR+  GK+L NE Q
Sbjct: 3   KKEKKANVSGKPKHSLDANRADGKKKTTETRSKSTVNRLKMYKTRPKRNAGGKILSNEYQ 62

Query: 64  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 123
           SKELP++RI PDRRWFGNTRVVNQKELE+FREELQ +MS +YNVILKE+KLP+SLL D++
Sbjct: 63  SKELPNSRIAPDRRWFGNTRVVNQKELEYFREELQTKMSSNYNVILKERKLPMSLLTDNK 122

Query: 124 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAE-G 183
           KQSRVHLLD EPF+DAFG K KRKRPKL+A+DYE+L+KKA +S DAFEE +    + E G
Sbjct: 123 KQSRVHLLDMEPFQDAFGRKTKRKRPKLVASDYEALVKKAAESQDAFEEKNGAGPSGEGG 182

Query: 184 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 243
            E DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDV+VQV+DARDPQGTRC+HLE+ LKE
Sbjct: 183 EEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVIVQVIDARDPQGTRCHHLEKTLKE 242

Query: 244 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 303
           H KHKH++LLLNKCDL+PAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR
Sbjct: 243 HHKHKHMILLLNKCDLVPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 302

Query: 304 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 363
           LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 303 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 362

Query: 364 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLI 423
           GVVYQ+ D+ETDIVLKGVVRVTNLEDASEHIGEVL+RVK+EHL+RAYKIKDWEDD+DFL+
Sbjct: 363 GVVYQSRDTETDIVLKGVVRVTNLEDASEHIGEVLRRVKKEHLQRAYKIKDWEDDHDFLL 422

Query: 424 QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDY---GVDDDS 483
           QLCK +GKLL+GGEPDL T AKM+LHDWQRGR+PFFVPPP++D+ + E +    G+D ++
Sbjct: 423 QLCKSSGKLLKGGEPDLMTGAKMILHDWQRGRIPFFVPPPKLDNVASESEVIVPGIDKEA 482

Query: 484 GVDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDT 543
             D++QAAAA KAIA ++S+QQQ++VPVQRD + + +L  D   +        E+  +D 
Sbjct: 483 IADNSQAAAALKAIAGIMSTQQQKDVPVQRDFYDEKDLKDDKKAK--------ESTETDA 542

Query: 544 EDKTSGDQDD 547
           E+ T  ++D+
Sbjct: 543 ENGTDAEEDE 544

BLAST of MC04g1657 vs. ExPASy Swiss-Prot
Match: A2XGQ1 (Nuclear/nucleolar GTPase 2 OS=Oryza sativa subsp. indica OX=39946 GN=NUG2 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 3.8e-235
Identity = 415/534 (77.72%), Postives = 460/534 (86.14%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNS----------RSAATVRRLKMYNTRPKRDR 60
           M KKKE+ VNVSGKP+HSLDVNR++  K +          RSAATVRRLKMY  RP RDR
Sbjct: 1   MAKKKERAVNVSGKPRHSLDVNRANDKKGAGGGAGGGGGGRSAATVRRLKMYKMRPLRDR 60

Query: 61  KGKVLKNELQSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKK 120
            GK+LK++LQSKELP+TRI+PDRRWFGNTRVVNQKELEFFREELQ R+S +YNVILKE+K
Sbjct: 61  GGKILKHDLQSKELPNTRIEPDRRWFGNTRVVNQKELEFFREELQSRLSNNYNVILKERK 120

Query: 121 LPLSLLNDHQKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEN 180
           LPLSLL DHQKQ+R HLLDTEPFE AFGPKGKRKRPKL+A DYESLLKKAD S  AFE+ 
Sbjct: 121 LPLSLLQDHQKQARAHLLDTEPFEHAFGPKGKRKRPKLMALDYESLLKKADDSQGAFEDK 180

Query: 181 HADNATAEGSEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRC 240
           HA     +  E DG RDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDP GTRC
Sbjct: 181 HATAKLLKEEEEDGLRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRC 240

Query: 241 YHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSL 300
           YHLE+HLKE+ KHKH+V LLNKCDL+PAWATKGWLR LSK+YPTLAFHAS+N SFGKGSL
Sbjct: 241 YHLEKHLKENAKHKHLVFLLNKCDLVPAWATKGWLRTLSKDYPTLAFHASINSSFGKGSL 300

Query: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLT 360
           LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLR+K+VCKVAPIPGETKVWQYITLT
Sbjct: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRSKSVCKVAPIPGETKVWQYITLT 360

Query: 361 KRIFLIDCPGVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKD 420
           KRIFLIDCPGVVYQN+DSETDIVLKGVVRVTNL DASEHIGEVL+RVK+EHL+RAYKI+D
Sbjct: 361 KRIFLIDCPGVVYQNNDSETDIVLKGVVRVTNLADASEHIGEVLRRVKKEHLKRAYKIED 420

Query: 421 WEDDNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRV-DDKSEEPD 480
           W DDNDFL+QL K TGKLLRGGEPDLTT AKMVLHDWQRG++PFFVPPP+  +D   E  
Sbjct: 421 WVDDNDFLVQLSKTTGKLLRGGEPDLTTTAKMVLHDWQRGKIPFFVPPPQQGEDSPSETA 480

Query: 481 YGVD--DDSGVDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQ 522
             VD  D+ GV S++ AAA KAIA +ISSQQQ NVP Q++ F  T  + + ++Q
Sbjct: 481 EPVDKSDEEGVSSDRTAAAMKAIAGIISSQQQMNVPCQKE-FGVTNEDSEVAEQ 533

BLAST of MC04g1657 vs. ExPASy Swiss-Prot
Match: Q10LF7 (Nuclear/nucleolar GTPase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NUG2 PE=1 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 1.1e-234
Identity = 414/534 (77.53%), Postives = 460/534 (86.14%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNS----------RSAATVRRLKMYNTRPKRDR 60
           M KKKE+ VNVSGKP+HSLDVNR++  K +          RSAATVRRLKMY  RP RDR
Sbjct: 1   MAKKKERAVNVSGKPRHSLDVNRANDKKGAGGGAGGGGGGRSAATVRRLKMYKMRPLRDR 60

Query: 61  KGKVLKNELQSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKK 120
            GK+LK++LQSKELP+TRI+PDRRWFGNTRVVNQKELEFFREELQ R+S +YNVILKE+K
Sbjct: 61  GGKILKHDLQSKELPNTRIEPDRRWFGNTRVVNQKELEFFREELQSRLSNNYNVILKERK 120

Query: 121 LPLSLLNDHQKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEN 180
           LPLSLL DHQKQ+R HLLDTEPFE AFGPKGKRKRPKL+A DYESLLKKAD S  AFE+ 
Sbjct: 121 LPLSLLQDHQKQARAHLLDTEPFEHAFGPKGKRKRPKLMALDYESLLKKADDSQGAFEDK 180

Query: 181 HADNATAEGSEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRC 240
           HA     +  E DG RDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDP GTRC
Sbjct: 181 HATAKLLKEEEEDGLRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRC 240

Query: 241 YHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSL 300
           YHLE+HLKE+ KHKH+V LLNKCDL+PAWATKGWLR LSK+YPTLAFHAS+N SFGKGSL
Sbjct: 241 YHLEKHLKENAKHKHLVFLLNKCDLVPAWATKGWLRTLSKDYPTLAFHASINSSFGKGSL 300

Query: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLT 360
           LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLR+K+VCKVAPIPGETKVWQYITLT
Sbjct: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRSKSVCKVAPIPGETKVWQYITLT 360

Query: 361 KRIFLIDCPGVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKD 420
           KRIFLIDCPGVVYQN+DSETDIVLKGVVRVTNL DASEHIGEVL+RVK+EHL+RAYKI+D
Sbjct: 361 KRIFLIDCPGVVYQNNDSETDIVLKGVVRVTNLADASEHIGEVLRRVKKEHLKRAYKIED 420

Query: 421 WEDDNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRV-DDKSEEPD 480
           W DDNDFL+QL K TGKLLRGGEPDLTT AKMVLHDWQRG++PFFVPPP+  +D   E  
Sbjct: 421 WVDDNDFLVQLSKTTGKLLRGGEPDLTTTAKMVLHDWQRGKIPFFVPPPQQGEDSPSETA 480

Query: 481 YGVD--DDSGVDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQ 522
             V+  D+ GV S++ AAA KAIA +ISSQQQ NVP Q++ F  T  + + ++Q
Sbjct: 481 EPVEKSDEEGVSSDRTAAAMKAIAGIISSQQQMNVPCQKE-FGVTNEDSEVAEQ 533

BLAST of MC04g1657 vs. ExPASy Swiss-Prot
Match: Q13823 (Nucleolar GTP-binding protein 2 OS=Homo sapiens OX=9606 GN=GNL2 PE=1 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 4.9e-142
Identity = 256/469 (54.58%), Postives = 337/469 (71.86%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNR--SDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKN- 60
           M K K K  +     K S + +R      +N R  AT+RRL MY  + +R+ +GK++K  
Sbjct: 1   MVKPKYKGRSTINPSKASTNPDRVQGAGGQNMRDRATIRRLNMYRQKERRNSRGKIIKPL 60

Query: 61  ELQSKELPDT--RIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSL 120
           + QS     T  R++P+ +WFGNTRV+ Q  L+ F+EE+   M   Y V++K+ KLP+SL
Sbjct: 61  QYQSTVASGTVARVEPNIKWFGNTRVIKQSSLQKFQEEMDTVMKDPYKVVMKQSKLPMSL 120

Query: 121 LNDHQK--QSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHAD 180
           L+D  +    +VH+LDTE FE  FGPK +RKRP L A+D +SL++ A+ S +++++    
Sbjct: 121 LHDRIRPHNLKVHILDTESFETTFGPKSQRKRPNLFASDMQSLIENAEMSTESYDQGKDR 180

Query: 181 NATAEGSEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHL 240
           +   E +   G R+  +  +++KGQSKRIWGELYKVIDSSDVVVQVLDARDP GTR  H+
Sbjct: 181 DLVTEDT---GVRNEAQEEIYKKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRSPHI 240

Query: 241 ERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSV 300
           E +LK+    KH++ +LNKCDL+P WATK W+ VLS++YPTLAFHAS+   FGKG+ + +
Sbjct: 241 ETYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLAFHASLTNPFGKGAFIQL 300

Query: 301 LRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRI 360
           LRQF +L +DK+ ISVGF+GYPNVGKSSVINTLR+K VC VAPI GETKVWQYITL +RI
Sbjct: 301 LRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQYITLMRRI 360

Query: 361 FLIDCPGVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWED 420
           FLIDCPGVVY + DSETDIVLKGVV+V  ++   +HIG VL+R K E++ + YKI  WE+
Sbjct: 361 FLIDCPGVVYPSEDSETDIVLKGVVQVEKIKSPEDHIGAVLERAKPEYISKTYKIDSWEN 420

Query: 421 DNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVD 463
             DFL +L   TGKLL+GGEPDL T  KMVL+DWQRGR+PFFV PP  +
Sbjct: 421 AEDFLEKLAFRTGKLLKGGEPDLQTVGKMVLNDWQRGRIPFFVKPPNAE 466

BLAST of MC04g1657 vs. ExPASy Swiss-Prot
Match: Q99LH1 (Nucleolar GTP-binding protein 2 OS=Mus musculus OX=10090 GN=Gnl2 PE=1 SV=2)

HSP 1 Score: 500.7 bits (1288), Expect = 2.1e-140
Identity = 276/584 (47.26%), Postives = 376/584 (64.38%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNR--SDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKN- 60
           M K K K  +   +   S + +R      +N R   T+RRL MY  + +R+ +GKV+K  
Sbjct: 1   MVKPKYKGRSTINRSAASTNPDRVQGAGGQNMRDRGTIRRLNMYRQKERRNSRGKVIKPL 60

Query: 61  ELQSKELPDT--RIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSL 120
           + QS     T  R++P+ +WFGNTRV+ Q  L+ F+EE+ K M   Y V++K+ KLP+SL
Sbjct: 61  QYQSTVASGTVARVEPNIKWFGNTRVIKQASLQKFQEEMDKVMKDPYKVVMKQSKLPMSL 120

Query: 121 LND--HQKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHAD 180
           L+D      ++VH+LDTE FE  FGPK +RKRP L A+D +SLL+ A+ S +++++    
Sbjct: 121 LHDRIQPHNAKVHILDTESFESTFGPKSQRKRPNLFASDMQSLLENAEMSTESYDQGKDR 180

Query: 181 NATAEGSEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHL 240
           +   E +   G R+  +  +++KGQSKRIWGELYKVIDSSDVVVQVLDARDP GTR  H+
Sbjct: 181 DLVMEDT---GVRNEAQEEIYKKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRSPHI 240

Query: 241 ERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSV 300
           E +LK+    KH++ +LNKCDL+P WATK W+ VLS++YPTLAFHAS+   FGKG+ + +
Sbjct: 241 EAYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLAFHASLTNPFGKGAFIQL 300

Query: 301 LRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRI 360
           LRQF +L +DK+ ISVGF+GYPNVGKSSVINTLR+K VC VAPI GETKVWQYITL +RI
Sbjct: 301 LRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQYITLMRRI 360

Query: 361 FLIDCPGVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWED 420
           FLIDCPGVVY + DSETDIVLKGVV+V  ++   +HIG VL+R K E++ + YKI+ WE+
Sbjct: 361 FLIDCPGVVYPSEDSETDIVLKGVVQVEKIKAPQDHIGAVLERAKPEYISKTYKIESWEN 420

Query: 421 DNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVD 480
             DFL +L   TGKLL+GGEPD+ T +KMVL+DWQRGR+PFFV PP  +         + 
Sbjct: 421 AEDFLEKLALRTGKLLKGGEPDMLTVSKMVLNDWQRGRIPFFVKPPNAE---------LP 480

Query: 481 DDSGVDSNQAAAAFKAIANVISSQQQRNVPVQR-DLFSDTELNGDAS-------DQILV- 540
            DS +  +               ++     V+R D  ++ E  GD S        QIL  
Sbjct: 481 TDSQLPPSSPLEVPTETTQNNPEEETTETEVERSDSITEKEPEGDCSQDRNSEMQQILAR 540

Query: 541 ---------------SEDELEAPLSDTEDKTSGDQDDVVEDQRP 554
                          ++D +   +SD ED  S  +++  E ++P
Sbjct: 541 VRQNFGKINVGPQFSADDLVPVEMSDLEDLESSGEEEEQEQEQP 572

BLAST of MC04g1657 vs. NCBI nr
Match: XP_022133818.1 (nuclear/nucleolar GTPase 2 isoform X1 [Momordica charantia])

HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ
Sbjct: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180
           KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS
Sbjct: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420
           VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ
Sbjct: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420

Query: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS 480
           LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS
Sbjct: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS 480

Query: 481 NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT 540
           NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT
Sbjct: 481 NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT 540

Query: 541 SGDQDDVVEDQRPTAG 556
           SGDQDDVVEDQRPTAG
Sbjct: 541 SGDQDDVVEDQRPTAG 556

BLAST of MC04g1657 vs. NCBI nr
Match: XP_022133820.1 (nuclear/nucleolar GTPase 2 isoform X2 [Momordica charantia])

HSP 1 Score: 1065 bits (2753), Expect = 0.0
Identity = 543/556 (97.66%), Postives = 543/556 (97.66%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ
Sbjct: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180
           KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSH             EGS
Sbjct: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSH-------------EGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420
           VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ
Sbjct: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420

Query: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS 480
           LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS
Sbjct: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS 480

Query: 481 NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT 540
           NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT
Sbjct: 481 NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT 540

Query: 541 SGDQDDVVEDQRPTAG 556
           SGDQDDVVEDQRPTAG
Sbjct: 541 SGDQDDVVEDQRPTAG 543

BLAST of MC04g1657 vs. NCBI nr
Match: TYK01950.1 (nuclear/nucleolar GTPase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1016 bits (2626), Expect = 0.0
Identity = 513/556 (92.27%), Postives = 535/556 (96.22%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRS-DANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNEL 60
           M KKKEKKVNVSGKPKHSLDVNRS + NKN RSAATVRRLKMYNTRPKRDRKGKVLKN+L
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRSAATVRRLKMYNTRPKRDRKGKVLKNDL 60

Query: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDH 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMS SYNVILKE+KLPLSLLNDH
Sbjct: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEG 180
           QKQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLL+KADKSHD FEE +A+NAT EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180

Query: 181 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SE DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLI 420
           GVVYQNSD+ETDIVLKGVVRVTNLEDASEHIGEVLKRVK+EHLERAYKIK+WEDDNDFL+
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNWEDDNDFLV 420

Query: 421 QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVD 480
           QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRG+LPFFVPPPRV+D+SEEP+YGVDDDSGVD
Sbjct: 421 QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGKLPFFVPPPRVEDESEEPNYGVDDDSGVD 480

Query: 481 SNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDK 540
           SNQAAAAFKAIANVISSQQQR+VPVQRDLFS+ ELNG+ SDQ LVSEDEL+APLSDTE K
Sbjct: 481 SNQAAAAFKAIANVISSQQQRSVPVQRDLFSENELNGETSDQTLVSEDELQAPLSDTEGK 540

Query: 541 TSGDQDDVVEDQRPTA 555
           TSGDQDD  ED+   A
Sbjct: 541 TSGDQDDKNEDEHTIA 556

BLAST of MC04g1657 vs. NCBI nr
Match: XP_038890331.1 (nuclear/nucleolar GTPase 2 [Benincasa hispida] >XP_038890332.1 nuclear/nucleolar GTPase 2 [Benincasa hispida])

HSP 1 Score: 1015 bits (2624), Expect = 0.0
Identity = 512/556 (92.09%), Postives = 535/556 (96.22%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           M KKKEKKVNVSGKPKHSLDVNRS+ NKN RSAATVRRLKMYNTRPKRDRKGKVLKNELQ
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNENKNGRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMS SYNVILKE+KLPLSLLNDHQ
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180
           KQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLL+KADKSHD FEE +A+NAT EGS
Sbjct: 121 KQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATIEGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           E DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420
           VVYQNSD+ETDIVLKGVVRVTNLEDASEHIGEVLKRVK+EHLERAYKIK+W DDNDFL+Q
Sbjct: 361 VVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKEWGDDNDFLVQ 420

Query: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS 480
           LCKLTGKLLRGGEPDLTTAAKMVLHDWQRG+LPFFV PPRV+D+SEEP+Y VD+DSGVDS
Sbjct: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGKLPFFVSPPRVEDESEEPNYCVDEDSGVDS 480

Query: 481 NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT 540
           NQAAAAFKAIANVISSQQQR+VPVQRDLFSD ELNG+ SD ILVSE+EL+AP SDTE KT
Sbjct: 481 NQAAAAFKAIANVISSQQQRSVPVQRDLFSDNELNGETSDHILVSENELQAPPSDTEGKT 540

Query: 541 SGDQDDVVEDQRPTAG 556
           SGD+DD  ED+RP AG
Sbjct: 541 SGDEDDDNEDERPIAG 556

BLAST of MC04g1657 vs. NCBI nr
Match: XP_022993572.1 (nuclear/nucleolar GTPase 2 [Cucurbita maxima] >XP_022993573.1 nuclear/nucleolar GTPase 2 [Cucurbita maxima] >XP_022993574.1 nuclear/nucleolar GTPase 2 [Cucurbita maxima])

HSP 1 Score: 1007 bits (2603), Expect = 0.0
Identity = 513/558 (91.94%), Postives = 536/558 (96.06%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKK E+KV+VSGKPKHSLD NRSD NKN+RSAATVRRLKMYNTRPKRDRKGKVLK+ELQ
Sbjct: 1   MTKKTERKVSVSGKPKHSLDANRSDGNKNARSAATVRRLKMYNTRPKRDRKGKVLKHELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMS SYNVILKEKKLPLSLLNDHQ
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSSSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180
           KQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLLKKADKSHD F+E +A+NAT EGS
Sbjct: 121 KQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLKKADKSHDDFDEKYAENATNEGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           E DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420
           VVYQNSD+ETD+VLKGVVRVTNLED SEHIGEVLKRVK+EHLERAYKIK+W DDNDFL+Q
Sbjct: 361 VVYQNSDTETDVVLKGVVRVTNLEDPSEHIGEVLKRVKKEHLERAYKIKNWVDDNDFLVQ 420

Query: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEE-PDYGVDDDSGVD 480
           LCKL+GKLLRGGEPDLTT AKMVLHDWQRG+LPFFVPPPRV+DKSEE P+Y VDDDSGVD
Sbjct: 421 LCKLSGKLLRGGEPDLTTVAKMVLHDWQRGKLPFFVPPPRVEDKSEEEPNYSVDDDSGVD 480

Query: 481 SNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDA-SDQILVSEDELEAPLSDTED 540
           SNQAAAAFKAIANVISSQQQR+VPVQRDLFSD ELNG++  DQILVSEDEL+AP SDTE 
Sbjct: 481 SNQAAAAFKAIANVISSQQQRSVPVQRDLFSDNELNGESCDDQILVSEDELQAP-SDTEG 540

Query: 541 KTSGDQDDVVEDQRPTAG 556
           +TS  QDDVVED RPTAG
Sbjct: 541 RTSEGQDDVVEDVRPTAG 557

BLAST of MC04g1657 vs. ExPASy TrEMBL
Match: A0A6J1C0B4 (Nuclear/nucleolar GTPase 2 OS=Momordica charantia OX=3673 GN=LOC111006286 PE=3 SV=1)

HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ
Sbjct: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180
           KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS
Sbjct: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420
           VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ
Sbjct: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420

Query: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS 480
           LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS
Sbjct: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS 480

Query: 481 NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT 540
           NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT
Sbjct: 481 NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT 540

Query: 541 SGDQDDVVEDQRPTAG 556
           SGDQDDVVEDQRPTAG
Sbjct: 541 SGDQDDVVEDQRPTAG 556

BLAST of MC04g1657 vs. ExPASy TrEMBL
Match: A0A6J1BWB7 (Nuclear/nucleolar GTPase 2 OS=Momordica charantia OX=3673 GN=LOC111006286 PE=3 SV=1)

HSP 1 Score: 1065 bits (2753), Expect = 0.0
Identity = 543/556 (97.66%), Postives = 543/556 (97.66%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ
Sbjct: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180
           KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSH             EGS
Sbjct: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSH-------------EGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420
           VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ
Sbjct: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420

Query: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS 480
           LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS
Sbjct: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDS 480

Query: 481 NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT 540
           NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT
Sbjct: 481 NQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKT 540

Query: 541 SGDQDDVVEDQRPTAG 556
           SGDQDDVVEDQRPTAG
Sbjct: 541 SGDQDDVVEDQRPTAG 543

BLAST of MC04g1657 vs. ExPASy TrEMBL
Match: A0A5D3BS64 (Nuclear/nucleolar GTPase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00410 PE=3 SV=1)

HSP 1 Score: 1016 bits (2626), Expect = 0.0
Identity = 513/556 (92.27%), Postives = 535/556 (96.22%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRS-DANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNEL 60
           M KKKEKKVNVSGKPKHSLDVNRS + NKN RSAATVRRLKMYNTRPKRDRKGKVLKN+L
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRSAATVRRLKMYNTRPKRDRKGKVLKNDL 60

Query: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDH 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMS SYNVILKE+KLPLSLLNDH
Sbjct: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEG 180
           QKQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLL+KADKSHD FEE +A+NAT EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180

Query: 181 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SE DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLI 420
           GVVYQNSD+ETDIVLKGVVRVTNLEDASEHIGEVLKRVK+EHLERAYKIK+WEDDNDFL+
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNWEDDNDFLV 420

Query: 421 QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVD 480
           QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRG+LPFFVPPPRV+D+SEEP+YGVDDDSGVD
Sbjct: 421 QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGKLPFFVPPPRVEDESEEPNYGVDDDSGVD 480

Query: 481 SNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDK 540
           SNQAAAAFKAIANVISSQQQR+VPVQRDLFS+ ELNG+ SDQ LVSEDEL+APLSDTE K
Sbjct: 481 SNQAAAAFKAIANVISSQQQRSVPVQRDLFSENELNGETSDQTLVSEDELQAPLSDTEGK 540

Query: 541 TSGDQDDVVEDQRPTA 555
           TSGDQDD  ED+   A
Sbjct: 541 TSGDQDDKNEDEHTIA 556

BLAST of MC04g1657 vs. ExPASy TrEMBL
Match: A0A6J1K2N0 (Nuclear/nucleolar GTPase 2 OS=Cucurbita maxima OX=3661 GN=LOC111489529 PE=3 SV=1)

HSP 1 Score: 1007 bits (2603), Expect = 0.0
Identity = 513/558 (91.94%), Postives = 536/558 (96.06%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKK E+KV+VSGKPKHSLD NRSD NKN+RSAATVRRLKMYNTRPKRDRKGKVLK+ELQ
Sbjct: 1   MTKKTERKVSVSGKPKHSLDANRSDGNKNARSAATVRRLKMYNTRPKRDRKGKVLKHELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMS SYNVILKEKKLPLSLLNDHQ
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSSSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180
           KQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLLKKADKSHD F+E +A+NAT EGS
Sbjct: 121 KQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLKKADKSHDDFDEKYAENATNEGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           E DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQ 420
           VVYQNSD+ETD+VLKGVVRVTNLED SEHIGEVLKRVK+EHLERAYKIK+W DDNDFL+Q
Sbjct: 361 VVYQNSDTETDVVLKGVVRVTNLEDPSEHIGEVLKRVKKEHLERAYKIKNWVDDNDFLVQ 420

Query: 421 LCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEE-PDYGVDDDSGVD 480
           LCKL+GKLLRGGEPDLTT AKMVLHDWQRG+LPFFVPPPRV+DKSEE P+Y VDDDSGVD
Sbjct: 421 LCKLSGKLLRGGEPDLTTVAKMVLHDWQRGKLPFFVPPPRVEDKSEEEPNYSVDDDSGVD 480

Query: 481 SNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDA-SDQILVSEDELEAPLSDTED 540
           SNQAAAAFKAIANVISSQQQR+VPVQRDLFSD ELNG++  DQILVSEDEL+AP SDTE 
Sbjct: 481 SNQAAAAFKAIANVISSQQQRSVPVQRDLFSDNELNGESCDDQILVSEDELQAP-SDTEG 540

Query: 541 KTSGDQDDVVEDQRPTAG 556
           +TS  QDDVVED RPTAG
Sbjct: 541 RTSEGQDDVVEDVRPTAG 557

BLAST of MC04g1657 vs. ExPASy TrEMBL
Match: A0A0A0KTZ3 (Nuclear/nucleolar GTPase 2 OS=Cucumis sativus OX=3659 GN=Csa_5G631500 PE=3 SV=1)

HSP 1 Score: 1005 bits (2598), Expect = 0.0
Identity = 507/552 (91.85%), Postives = 532/552 (96.38%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRS-DANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNEL 60
           M KKKEKKVNVSGKPKHSLDVNRS + NKN R+AATVRRLKMYNTRPKRDRKGKVLKN+L
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60

Query: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDH 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMS SYNVILKE+KLPLSLLNDH
Sbjct: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEG 180
           QKQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLL+KADKSHD FEE +A+NAT EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180

Query: 181 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SE DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLI 420
           GVVYQNSD+ETDIVLKGVVRVTNLEDASEHIGEVLKRVK+EHLERAYKIK+WEDDNDFL+
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNWEDDNDFLV 420

Query: 421 QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVD 480
           QLCKL+GKLL+GGEPDLTTAAKMVLHDWQRG+LPFFVPPPRV+D+SEEP+Y VDDDSGVD
Sbjct: 421 QLCKLSGKLLKGGEPDLTTAAKMVLHDWQRGKLPFFVPPPRVEDESEEPNYCVDDDSGVD 480

Query: 481 SNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDK 540
           SNQAAAAFKAIANVISSQQQR+VPVQRDLFS+ ELNG+ SDQILVSEDEL+APLSDTE K
Sbjct: 481 SNQAAAAFKAIANVISSQQQRSVPVQRDLFSENELNGETSDQILVSEDELQAPLSDTEGK 540

Query: 541 TSGDQDDVVEDQ 551
           T GDQDD  ED+
Sbjct: 541 TLGDQDDKNEDE 552

BLAST of MC04g1657 vs. TAIR 10
Match: AT1G52980.1 (GTP-binding family protein )

HSP 1 Score: 818.1 bits (2112), Expect = 4.2e-237
Identity = 411/550 (74.73%), Postives = 476/550 (86.55%), Query Frame = 0

Query: 4   KKEKKVNVSGKPKHSLDVNRSDANK---NSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 63
           KKEKK NVSGKPKHSLD NR+D  K    +RS +TV RLKMY TRPKR+  GK+L NE Q
Sbjct: 3   KKEKKANVSGKPKHSLDANRADGKKKTTETRSKSTVNRLKMYKTRPKRNAGGKILSNEYQ 62

Query: 64  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 123
           SKELP++RI PDRRWFGNTRVVNQKELE+FREELQ +MS +YNVILKE+KLP+SLL D++
Sbjct: 63  SKELPNSRIAPDRRWFGNTRVVNQKELEYFREELQTKMSSNYNVILKERKLPMSLLTDNK 122

Query: 124 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAE-G 183
           KQSRVHLLD EPF+DAFG K KRKRPKL+A+DYE+L+KKA +S DAFEE +    + E G
Sbjct: 123 KQSRVHLLDMEPFQDAFGRKTKRKRPKLVASDYEALVKKAAESQDAFEEKNGAGPSGEGG 182

Query: 184 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 243
            E DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDV+VQV+DARDPQGTRC+HLE+ LKE
Sbjct: 183 EEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVIVQVIDARDPQGTRCHHLEKTLKE 242

Query: 244 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 303
           H KHKH++LLLNKCDL+PAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR
Sbjct: 243 HHKHKHMILLLNKCDLVPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 302

Query: 304 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 363
           LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 303 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 362

Query: 364 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLI 423
           GVVYQ+ D+ETDIVLKGVVRVTNLEDASEHIGEVL+RVK+EHL+RAYKIKDWEDD+DFL+
Sbjct: 363 GVVYQSRDTETDIVLKGVVRVTNLEDASEHIGEVLRRVKKEHLQRAYKIKDWEDDHDFLL 422

Query: 424 QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDY---GVDDDS 483
           QLCK +GKLL+GGEPDL T AKM+LHDWQRGR+PFFVPPP++D+ + E +    G+D ++
Sbjct: 423 QLCKSSGKLLKGGEPDLMTGAKMILHDWQRGRIPFFVPPPKLDNVASESEVIVPGIDKEA 482

Query: 484 GVDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDT 543
             D++QAAAA KAIA ++S+QQQ++VPVQRD + + +L  D   +        E+  +D 
Sbjct: 483 IADNSQAAAALKAIAGIMSTQQQKDVPVQRDFYDEKDLKDDKKAK--------ESTETDA 542

Query: 544 EDKTSGDQDD 547
           E+ T  ++D+
Sbjct: 543 ENGTDAEEDE 544

BLAST of MC04g1657 vs. TAIR 10
Match: AT3G07050.1 (GTP-binding family protein )

HSP 1 Score: 209.5 bits (532), Expect = 6.8e-54
Identity = 129/396 (32.58%), Postives = 211/396 (53.28%), Query Frame = 0

Query: 180 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 239
           +EG+   DL +         +  + EL KVI+ SDV+++VLDARDP GTRC  +ER + +
Sbjct: 104 TEGETIEDLPKVVNVRDNSERAFYKELVKVIELSDVILEVLDARDPLGTRCTDMERMVMQ 163

Query: 240 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKS--------------- 299
              +KH+VLLLNK DL+P  A + WL  L +E+P +AF  S  +                
Sbjct: 164 AGPNKHLVLLLNKIDLVPREAAEKWLMYLREEFPAVAFKCSTQEQRSNLGWKSSKASKPS 223

Query: 300 --------FGKGSLLSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAP 359
                    G  +L+ +L+ ++R    K++I+VG +G PNVGKSS+IN+L+  +V  V  
Sbjct: 224 NMLQTSDCLGADTLIKLLKNYSRSHELKKSITVGIIGLPNVGKSSLINSLKRAHVVNVGA 283

Query: 360 IPGETKVWQYITLTKRIFLIDCPGVV-YQNSDSETDIVLKGVVRVTNLEDASEHIGEVLK 419
            PG T+  Q + L K + L+DCPGVV  ++S ++  I L+   R+  L+D    + E+LK
Sbjct: 284 TPGLTRSLQEVHLDKNVKLLDCPGVVMLKSSGNDASIALRNCKRIEKLDDPVSPVKEILK 343

Query: 420 RVKREHLERAYKIKDWEDDNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFF 479
              ++ L   YKI  +E  +DFL ++  + GKL +GG  D+  AA++VLHDW  G++P++
Sbjct: 344 LCPKDMLVTLYKIPSFEAVDDFLYKVATVRGKLKKGGLVDIDAAARIVLHDWNEGKIPYY 403

Query: 480 VPPPRVDDKSEEPDYGVDD---DSGVDS-NQAAAAFKAIANVISSQQQRNVPVQRDL-FS 539
             PP+ D         V +   D  +D      ++F      ++      +P    L F 
Sbjct: 404 TMPPKRDQGGHAESKIVTELAKDFNIDEVYSGESSFIGSLKTVNEFNPVIIPSNGPLNFD 463

Query: 540 DTELNGDASDQILVSEDELEAPLSDTEDKTSGDQDD 547
           +T +  ++  Q   +E+E E   SD ++   G++++
Sbjct: 464 ETMIEDESKTQ---TEEEAEHE-SDDDESMGGEEEE 495

BLAST of MC04g1657 vs. TAIR 10
Match: AT2G27200.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 152.1 bits (383), Expect = 1.3e-36
Identity = 105/352 (29.83%), Postives = 169/352 (48.01%), Query Frame = 0

Query: 194 FEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVLLLNKC 253
           FEK     IW +L++V++ SD++V V+DARDP   RC  LE + +E  +HK  +LL+NK 
Sbjct: 151 FEKNLD--IWRQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEIDEHKKTMLLVNKA 210

Query: 254 DLIPAWATKGWLRVLSKEYPTLAFHAS----------------------------VNKSF 313
           DL+P++  + W    S+      F ++                              K +
Sbjct: 211 DLLPSYVREKWAEYFSRNNILFVFWSAKAATATLEGKPLKEQWRAPDTTQKTDNPAVKVY 270

Query: 314 GKGSLLSVLR----QFARLKSDK-----------QAISVGFVGYPNVGKSSVINTLRTKN 373
           G+  LL  L+    +  +++  +           + + VGFVGYPNVGKSS IN L  + 
Sbjct: 271 GRDDLLDRLKLEALEIVKMRKSRGVSATSTESHCEQVVVGFVGYPNVGKSSTINALVGQK 330

Query: 374 VCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQN-SDSETDIVLKGVVRVTNLEDASEH 433
              V   PG+TK +Q + +++ + L DCPG+V+ + S S  ++V  GV+ +  + +  E 
Sbjct: 331 RTGVTSTPGKTKHFQTLIISEDLMLCDCPGLVFPSFSSSRYEMVASGVLPIDRMTEHLEA 390

Query: 434 IGEVLKRVKREHLERAYKI-----KDWEDD------NDFLIQLCKLTGKLLRGGEPDLTT 489
           I  V + V R  +E  Y I     K +E        ++ L   C   G +   G PD T 
Sbjct: 391 IKVVAELVPRHAIEDVYNISLPKPKSYEPQSRPPLASELLRTYCLSRGYVASSGLPDETR 450

BLAST of MC04g1657 vs. TAIR 10
Match: AT1G08410.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 144.8 bits (364), Expect = 2.0e-34
Identity = 99/335 (29.55%), Postives = 161/335 (48.06%), Query Frame = 0

Query: 194 FEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVLLLNKC 253
           FEK     IW +L++V++ SD++V V+DARDP   RC  LE + +E  +HK ++LL+NK 
Sbjct: 151 FEKNLD--IWRQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEIDEHKKIMLLVNKA 210

Query: 254 DLIPAWATKGWLRVLSKEYPTLAFHASVNKS----------------------------F 313
           DL+P    + W            F +++  +                            +
Sbjct: 211 DLLPTDVREKWAEYFRLNNILFVFWSAIAATATLEGKVLKEQWRQPDNLQKTDDPDIMIY 270

Query: 314 GKGSLLSVL----RQFARLKSDKQA---------------ISVGFVGYPNVGKSSVINTL 373
           G+  LLS L    ++  ++++ + A                 VGFVGYPNVGKSS IN L
Sbjct: 271 GRDELLSRLQFEAQEIVKVRNSRAASVSSQSWTGEYQRDQAVVGFVGYPNVGKSSTINAL 330

Query: 374 RTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQN-SDSETDIVLKGVVRVTNLED 433
             +    V   PG+TK +Q + ++  + L DCPG+V+ + S S  +++  GV+ +  + +
Sbjct: 331 VGQKRTGVTSTPGKTKHFQTLIISDELMLCDCPGLVFPSFSSSRYEMIASGVLPIDRMTE 390

Query: 434 ASEHIGEVLKRVKREHLERAYKI-----KDWEDDN------DFLIQLCKLTGKLLRGGEP 470
             E I  V  +V R  +E  Y I     K +E  +      + L   C   G +   G P
Sbjct: 391 HREAIQVVADKVPRRVIESVYNISLPKPKTYERQSRPPHAAELLKSYCASRGYVASSGLP 450

BLAST of MC04g1657 vs. TAIR 10
Match: AT4G02790.1 (GTP-binding family protein )

HSP 1 Score: 85.5 bits (210), Expect = 1.5e-16
Identity = 68/266 (25.56%), Postives = 122/266 (45.86%), Query Frame = 0

Query: 205 ELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGW 264
           EL + +   DVV++V DAR P  T    ++  L     ++  +L+LN+ D+I       W
Sbjct: 109 ELREQLKLMDVVIEVRDARIPLSTTHPKMDAWL----GNRKRILVLNREDMISNDDRNDW 168

Query: 265 LRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARLKSD-----------KQAISVGFVG 324
            R  +K+   + F    N   G G+ + + R    L  D            +++  G +G
Sbjct: 169 ARYFAKQGIKVIF---TNGKLGMGA-MKLGRLAKSLAGDVNGKRREKGLLPRSVRAGIIG 228

Query: 325 YPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQNSDSETDIV 384
           YPNVGKSS+IN L  + +C  AP PG T+  +++ L K + L+D PG++    D +   +
Sbjct: 229 YPNVGKSSLINRLLKRKICAAAPRPGVTREMKWVKLGKDLDLLDSPGMLPMRIDDQAAAI 288

Query: 385 LKGVV-----RVTNLEDASEHIGEVLKRVKREHLERAYKIKDWEDDNDFLIQLCKLTGKL 444
              +      +  +  D +  + ++L R+     +  Y     + + +   +  K  G  
Sbjct: 289 KLAICDDIGEKAYDFTDVAGILVQMLARIPEVGAKALYNRYKIQLEGNCGKKFVKTLGLN 348

Query: 445 LRGGEPDLTTAAKMVLHDWQRGRLPF 455
           L GG  D   AA  +L D+++G+  +
Sbjct: 349 LFGG--DSHQAAFRILTDFRKGKFGY 364