MC04g1598 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g1598 vs. ExPASy Swiss-Prot
Match: Q6F4F5 (Cytochrome P450 724B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP724B1 PE=1 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.8e-127
Identity = 235/461 (50.98%), Postives = 321/461 (69.63%), Query Frame = 0

Query: 2   ELVAAAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTL 61
           ELV AA        LVI L+L +  ++SH L  L         P  P GS G+P LG+TL
Sbjct: 5   ELVLAA--------LVILLALLLTLVLSHFLPLLL-------NPKAPKGSFGWPLLGETL 64

Query: 62  GLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYP 121
             L PH SN++G+FL  HCSRYG+VFKSHLF  P IVSCDQELN FILQN+++LF+ SYP
Sbjct: 65  RFLSPHASNTLGSFLEDHCSRYGRVFKSHLFCTPTIVSCDQELNHFILQNEERLFQCSYP 124

Query: 122 KAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQ-- 181
           + +HGILG +S+++  G+ H++LRN+ ++ +   K +PS+L  +  + + ++ SW  +  
Sbjct: 125 RPIHGILGKSSMLVVLGEDHKRLRNLALALVTSTKLKPSYLGDIEKIALHIVGSWHGKSK 184

Query: 182 -----NQVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGT 241
                N + FC+E + FA S++VK++LG+  +E + + ILE+F  +MKG +S P+ +PGT
Sbjct: 185 DKGMVNVIAFCEEARKFAFSVIVKQVLGLSPEEPVTAMILEDFLAFMKGLISFPLYIPGT 244

Query: 242 AYSNAVKARERLSCMVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILL 301
            Y+ AV+AR R+S  VK ++ ER+  G       DFL V++S+ +L+DEE VS VLD LL
Sbjct: 245 PYAKAVQARARISSTVKGIIEERRNAGS--SNKGDFLDVLLSSNELSDEEKVSFVLDSLL 304

Query: 302 GGYETTATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVM 361
           GGYETT+ L+ ++VYFL  S  + L  +K EHE IR +K   E L+ EDYK+ME+T +V+
Sbjct: 305 GGYETTSLLISMVVYFLGQSAQD-LELVKREHEGIRSKKEKDEFLSSEDYKKMEYTQHVI 364

Query: 362 FEAMRCGNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWT 421
            EA+RCGN+VKF+HR+A+KDV++KE +IPSGWKV PVFSAVHL+P LH N QQF P RW 
Sbjct: 365 NEALRCGNIVKFVHRKALKDVRYKEYLIPSGWKVLPVFSAVHLNPLLHGNAQQFQPCRWE 424

Query: 422 DDKE-MGKKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 455
              +   KK TPFGGGPRLCPG ELAK+E +FFLHHLVLNY
Sbjct: 425 GASQGTSKKFTPFGGGPRLCPGSELAKVEAAFFLHHLVLNY 447

BLAST of MC04g1598 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 355.5 bits (911), Expect = 1.6e-96
Identity = 184/449 (40.98%), Postives = 275/449 (61.25%), Query Frame = 0

Query: 46  NLPPGSMGFPFLGQTLGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELN 105
           NLPPG  G+PFLG+T+G LKP+ + ++G F+  H S+YGK+++S+LFG P IVS D  LN
Sbjct: 38  NLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLN 97

Query: 106 MFILQNDDKLFKVSYPKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCV 165
            FILQN+ +LF+ SYP+++ GILG  S+++  GD HR +R++ ++F+   + R   L  V
Sbjct: 98  RFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDV 157

Query: 166 HNLTISLMDSWRSQNQVFFCK-EVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGF 225
              T+ ++DSW+ QN +F  + E K F  ++M K ++ ++  E    ++ +E+  +MKG 
Sbjct: 158 ERHTLFVLDSWQ-QNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGV 217

Query: 226 VSLPINLPGTAYSNAVKARERLSCMVKEMMRERK------------------------RR 285
           VS P+NLPGTAY  A+++R  +   ++  M ERK                          
Sbjct: 218 VSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDH 277

Query: 286 GMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETTATLMGLIVYFLAHSPPNVLA 345
                 D+D L  ++ +  L+ E+I+ ++L +L  G+ET++  + L ++FL  + P  + 
Sbjct: 278 VRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFL-QACPKAVE 337

Query: 346 KLKEEH-EAIRIRKRNGES-LNLEDYKQMEFTNNVMFEAMRCGNVVKFLHREAVKDVKFK 405
           +L+EEH E  R +K  GES LN +DYK+M+FT  V+ E +R GNVV+FLHR+A+KDV++K
Sbjct: 338 ELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRFLHRKALKDVRYK 397

Query: 406 EIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKE------------MGKKVTPF 456
              IPSGWKV PV SAVHLD + +  P  FNPWRW                  G    PF
Sbjct: 398 GYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGSGSFSTWGNNYMPF 457

BLAST of MC04g1598 vs. ExPASy Swiss-Prot
Match: Q5CCK3 (Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 2.0e-91
Identity = 171/419 (40.81%), Postives = 261/419 (62.29%), Query Frame = 0

Query: 46  NLPPGSMGFPFLGQTLGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELN 105
           NLPPG+ G+P +G+T G L+ HP+ S+G F+  H +RYGK+++S LFG   +VS D  LN
Sbjct: 53  NLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGERTVVSADAGLN 112

Query: 106 MFILQNDDKLFKVSYPKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCV 165
            +ILQN+ +LF+ SYP+++ GILG  S+++  GD HR++R + ++F+   + R   L  V
Sbjct: 113 RYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLSSVRLRAVLLPEV 172

Query: 166 HNLTISLMDSWRSQNQVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFV 225
              T+ ++ +W   +      + K F  ++M K ++ ++  E    ++  E+  +MKG V
Sbjct: 173 ERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETERLRREYITFMKGVV 232

Query: 226 SLPINLPGTAYSNAVKARERLSCMVKEMMRERKRRGMVVGA---DEDFLQVIMSNWKLND 285
           S P+NLPGT Y  A+K+R  +  +++  M ER  +     A    +D L   +    L+ 
Sbjct: 233 SAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVEQDDLLGWALKQSNLSK 292

Query: 286 EEIVSVVLDILLGGYETTATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKR-NGE-SLN 345
           E+I+ ++L +L  G+ET++  + L ++FL    P  + +L+EEH  I  R+R  GE  L+
Sbjct: 293 EQILDLLLSLLFAGHETSSMALALAIFFL-EGCPKAVQELREEHLGIARRQRLRGECKLS 352

Query: 346 LEDYKQMEFTNNVMFEAMRCGNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPA 405
            EDYK+M FT  V+ E +R GNVV+FLHR+ +KDV +K   IPSGWK+ PV +AVHLD +
Sbjct: 353 WEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYDIPSGWKILPVLAAVHLDSS 412

Query: 406 LHPNPQQFNPWRWTDDKEMG-----KKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 455
           L+ +PQ+FNPWRW      G         P+GGG RLC G ELAKLE++ FLHHLVLN+
Sbjct: 413 LYEDPQRFNPWRWKSSGSSGGLAQSSSFMPYGGGTRLCAGSELAKLEMAVFLHHLVLNF 470

BLAST of MC04g1598 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.4e-76
Identity = 167/448 (37.28%), Postives = 253/448 (56.47%), Query Frame = 0

Query: 19  ALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLKPHPSNSIGTFLHH 78
           A  L +++I +  LL L   +       LPPGS+G P +G+T  L+  + + +   F+  
Sbjct: 5   AFLLLLSSIAAGFLLLLR--RTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDE 64

Query: 79  HCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMHGILGTNSLIIAAG 138
             +RYG VF +HLFG P I S D E N F+LQN+ KLF+ SYP ++  +LG +SL++  G
Sbjct: 65  RVARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKG 124

Query: 139 DTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFCKEVKMFALSIMVK 198
             H+++ ++ +SF      +   +  +  L    +DSW S  +V   +E K     + VK
Sbjct: 125 SLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSS--RVLLMEEAKKITFELTVK 184

Query: 199 ELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARERLS-CMVKEMMRER 258
           +L+  +  E     + +E+   ++GF SLP+ L  T Y  A++AR +++  +   +M+ R
Sbjct: 185 QLMSFDPGE-WSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRR 244

Query: 259 KRRGMVVGADEDFLQVIM-SNWKLNDEEIVSVVLDILLGGYETTATLMGLIVYFLAHSPP 318
           +         +D L  ++ ++   +DEEIV  ++ +L+ GYETT+T+M L V FL  + P
Sbjct: 245 EEEEEGAERKKDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTET-P 304

Query: 319 NVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRCGNVVKFLHREAVKDVK 378
             LA+LKEEHE IR  K +  SL   DYK M FT  V+ E +R  N++  + R A+ DV+
Sbjct: 305 LALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVE 364

Query: 379 FKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEM---GKKVTPFGGGPRLC 438
            K   IP GWKV   F AVHLDP    + + FNPWRW  +          TPFGGGPRLC
Sbjct: 365 IKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLC 424

Query: 439 PGVELAKLEISFFLHHLVLNYS-MPKEE 461
           PG ELA++ +S FLH LV  +S +P E+
Sbjct: 425 PGYELARVALSVFLHRLVTGFSWVPAEQ 446

BLAST of MC04g1598 vs. ExPASy Swiss-Prot
Match: Q50EK6 (Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 6.2e-72
Identity = 144/412 (34.95%), Postives = 227/412 (55.10%), Query Frame = 0

Query: 46  NLPPGSMGFPFLGQTLGLLKPHPSNSIGTFLHHHCSRY-GKVFKSHLFGWPAIVSCDQEL 105
           +LPPGS G+P +G+T    +   SN    F+     RY   VF SHLFG  A++S D + 
Sbjct: 44  HLPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSDVFVSHLFGSQAVISSDPQF 103

Query: 106 NMFILQNDDKLFKVSYPKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHC 165
           N ++LQN+ + F+  YPKA+  ++G   L+   GD  RKL  + V+ + + + +  F+  
Sbjct: 104 NKYVLQNEGRFFQAHYPKALKALIGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEE 163

Query: 166 VHNLTISLMDSWRSQNQVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGF 225
           + NL  S +D W  + ++    E     L++M K+LL +   +   ++I E F  Y    
Sbjct: 164 IQNLVHSTLDRWVDKKEIALQNECHQMVLNLMAKQLLDLSPSKET-NEICELFVDYTNAV 223

Query: 226 VSLPINLPGTAYSNAVKARERLSCMVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEE 285
           +++PI +PG+ Y+  +KARE L   +  M++ER+    +V   +D L  ++    ++DE 
Sbjct: 224 IAIPIKIPGSTYAKGLKARELLIRKISNMIKERRDHPHIV--HKDLLTKLLEEDSISDEI 283

Query: 286 IVSVVLDILLGGYETTATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDY 345
           I   +L +L  G+ET++  M   + FL  + P  L ++KEEH+AI   K   + L  +DY
Sbjct: 284 ICDFILFLLFAGHETSSRAMTFAIKFLT-TCPKALTQMKEEHDAILKAKGGHKKLEWDDY 343

Query: 346 KQMEFTNNVMFEAMRCGNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPN 405
           K M+FT  V+ E +R GN    + RE  +D K K+ +IP GW V    +A HLD   H  
Sbjct: 344 KSMKFTQCVINETLRLGNFGPGVFRETKEDTKVKDCLIPKGWVVFAFLTATHLDEKFHNE 403

Query: 406 PQQFNPWRWTDDKEMGKK--VTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 455
              FNPWRW  D+++      +PFGGG RLCPG  LA+LE++ FLH  +  +
Sbjct: 404 ALTFNPWRWELDQDVSNNHLFSPFGGGARLCPGSHLARLELALFLHIFITRF 451

BLAST of MC04g1598 vs. NCBI nr
Match: KAG6602402.1 (Cytochrome P450 724B1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1236 bits (3199), Expect = 0.0
Identity = 668/877 (76.17%), Postives = 722/877 (82.33%), Query Frame = 0

Query: 6   AAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLK 65
           A AS SP TI   ALSLAMAA+I   LL L +  AA   P+LPPG MGFPF+G+TL  LK
Sbjct: 3   APASFSPLTI---ALSLAMAAVILCQLL-LKLFAAATPSPSLPPGCMGFPFVGETLSFLK 62

Query: 66  PHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMH 125
           PH SNSIGTFL  HCSR+GKVFKSHLFG  AIVSCD+ELNMFILQNDDKLFKVSYPKAMH
Sbjct: 63  PHHSNSIGTFLQRHCSRFGKVFKSHLFGSWAIVSCDRELNMFILQNDDKLFKVSYPKAMH 122

Query: 126 GILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFC 185
           GILG+NSLIIAAGDTHRKLRNVVVSF+ WCKS P+FLHCV  L++SLMDSWRSQ +V FC
Sbjct: 123 GILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFC 182

Query: 186 KEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARER 245
           KEVKMFALS+MVKELLGIEA E+IGS+IL+EFE YMKGFVSLP+NL GT Y  AVKAR +
Sbjct: 183 KEVKMFALSVMVKELLGIEANESIGSRILDEFETYMKGFVSLPLNLLGTPYYKAVKARAK 242

Query: 246 LSCMVKEMMRERKRRGMVVGAD------EDFLQVIMSNWKLNDEEIVSVVLDILLGGYET 305
           LS +VKEMMRERK++G+VVG +      E+FL VIMSNWKL++EEIVSVVLDILLGGYET
Sbjct: 243 LSGIVKEMMRERKKKGLVVGVEKERDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYET 302

Query: 306 TATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMR 365
           TATLMGLIVYFLAHSPP+VLAKLKEEHE IR  K  GE LNLEDYKQMEFT NV++EAMR
Sbjct: 303 TATLMGLIVYFLAHSPPHVLAKLKEEHEGIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMR 362

Query: 366 CGNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEM 425
           CGNVVKFLHREA+KDVKFK+I IPSGWKVHPVFSA+HLDP LHP PQQFNPWRW DDKEM
Sbjct: 363 CGNVVKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEM 422

Query: 426 GKKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNYS------------------------ 485
            KKVTPFGGGPRLCPG+ELAKLEI+FFLHHLVLNY                         
Sbjct: 423 SKKVTPFGGGPRLCPGIELAKLEIAFFLHHLVLNYRWKTSADECPLAYPYLQSLFRLFIR 482

Query: 486 -----------------MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMK 545
                            MPKEE  EV LTTD SPESSKK  K SK KKKQLL EGVDVM+
Sbjct: 483 AAHCRPLFLSLARVTARMPKEESFEVGLTTDCSPESSKK-LKGSKKKKKQLLVEGVDVME 542

Query: 546 MKEVRSVQVEESNINSDGDKVKKKKKGKKSAKQCVEENSIKAEAGEIESKAESSMKEGGP 605
                S ++ ESNINS+   VK KKK KK   QCV ENSI+AEAGE E KAE S+KEG P
Sbjct: 543 RNVEGSFELWESNINSE---VKNKKKRKKITIQCVVENSIQAEAGEYEIKAERSVKEGSP 602

Query: 606 FSDGGGEKDAAFEKKQIPLEHAEDARADSIVRENSGEKILESNNGKSDQR-IKRKKRLLK 665
            SDGG EK+   E++Q  LE+ E+ RADSIVRE SGEKILESNNGK+DQR IKRKKRLLK
Sbjct: 603 LSDGGSEKNEILEERQFALENDENERADSIVRETSGEKILESNNGKTDQRNIKRKKRLLK 662

Query: 666 ESAKANMCGICYLSRVPPHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQ 725
           E A A+M GICYLSRVPPHMDPLKLRQILS YGEIQRIYLAPEDAANQV+RKRAGGFRGQ
Sbjct: 663 EVANADMRGICYLSRVPPHMDPLKLRQILSQYGEIQRIYLAPEDAANQVKRKRAGGFRGQ 722

Query: 726 LFSEGWVEFTDKRVAKKVATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYK 785
            FSEGWVEFTDKRVAKKVA MLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYK
Sbjct: 723 FFSEGWVEFTDKRVAKKVANMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYK 782

Query: 786 HAIREQKLALEISAAKRERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVYQK 834
           HAIREQK+ALEISAAKRERDFYLSKVDKSRAL SIEERLKKKQKLREDSEM+SE    QK
Sbjct: 783 HAIREQKMALEISAAKRERDFYLSKVDKSRALKSIEERLKKKQKLREDSEMNSELADSQK 842

BLAST of MC04g1598 vs. NCBI nr
Match: XP_022133522.1 (cytochrome P450 724B1 [Momordica charantia])

HSP 1 Score: 910 bits (2353), Expect = 0.0
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0

Query: 1   MELVAAAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQT 60
           MELVAAAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQT
Sbjct: 1   MELVAAAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQT 60

Query: 61  LGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSY 120
           LGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSY
Sbjct: 61  LGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSY 120

Query: 121 PKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQN 180
           PKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQN
Sbjct: 121 PKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQN 180

Query: 181 QVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAV 240
           QVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAV
Sbjct: 181 QVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAV 240

Query: 241 KARERLSCMVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETT 300
           KARERLSCMVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETT
Sbjct: 241 KARERLSCMVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETT 300

Query: 301 ATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRC 360
           ATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRC
Sbjct: 301 ATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRC 360

Query: 361 GNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEMG 420
           GNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEMG
Sbjct: 361 GNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEMG 420

Query: 421 KKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 454
           KKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY
Sbjct: 421 KKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 454

BLAST of MC04g1598 vs. NCBI nr
Match: XP_038889747.1 (cytochrome P450 724B1 [Benincasa hispida])

HSP 1 Score: 760 bits (1963), Expect = 5.84e-268
Identity = 370/452 (81.86%), Postives = 406/452 (89.82%), Query Frame = 0

Query: 9   SLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLKPHP 68
           S SP  I+V+ALS A+  IISHSLLKL VI  +   PNLPPG MGFPF+G+TL  LKPH 
Sbjct: 7   SFSPMIIIVLALSFAIL-IISHSLLKLFVITTSTPNPNLPPGCMGFPFVGETLSFLKPHH 66

Query: 69  SNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMHGIL 128
           SNSIGTFL HHCSRYGKVFKSHLFGWPAIVSCD+ELN+FILQNDDKLFKVSYPKAMHGIL
Sbjct: 67  SNSIGTFLQHHCSRYGKVFKSHLFGWPAIVSCDRELNIFILQNDDKLFKVSYPKAMHGIL 126

Query: 129 GTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFCKEV 188
           G+NSLIIAAG THRKLRNVVVSF  WCKSRP+FLH +H+L+ISLMDSWRSQ Q+ FCK+V
Sbjct: 127 GSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPTFLHSLHSLSISLMDSWRSQTQLLFCKQV 186

Query: 189 KMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARERLSC 248
           K+FALS+MVKEL GIEAKE+IGSKIL+EFE YMKGFVSLP+ LPGT YS AVKARERLS 
Sbjct: 187 KLFALSVMVKELFGIEAKESIGSKILKEFETYMKGFVSLPLKLPGTPYSKAVKARERLSY 246

Query: 249 MVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETTATLMGLIV 308
           +VKEMMRER+RRG++ G DEDFLQVIMSNWKL+DEEIVSVVLDILLGGYETTATLMGLI+
Sbjct: 247 IVKEMMRERRRRGLLEGNDEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETTATLMGLII 306

Query: 309 YFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRCGNVVKFLH 368
           YFL+HSPPNVLAKLKEEH+AIR  KRNGE LNLEDYKQMEFT NV+FEAMRCGNVVKFLH
Sbjct: 307 YFLSHSPPNVLAKLKEEHQAIRFGKRNGECLNLEDYKQMEFTCNVIFEAMRCGNVVKFLH 366

Query: 369 REAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEMGKKVTPFGG 428
           REA+ DVKFK+IVIP GWKVHPVFSA+HLDP +HPNPQ FNPWRWTDDK+M KKVTPFGG
Sbjct: 367 REALTDVKFKDIVIPCGWKVHPVFSAIHLDPTIHPNPQHFNPWRWTDDKDMSKKVTPFGG 426

Query: 429 GPRLCPGVELAKLEISFFLHHLVLNYSMPKEE 460
           GPRLCPG+ELAKLEI+FFLHH VLNY     E
Sbjct: 427 GPRLCPGIELAKLEIAFFLHHFVLNYRWKTRE 457

BLAST of MC04g1598 vs. NCBI nr
Match: XP_023550580.1 (cytochrome P450 724B1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 722 bits (1863), Expect = 8.41e-253
Identity = 362/455 (79.56%), Postives = 398/455 (87.47%), Query Frame = 0

Query: 6   AAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLK 65
           A AS SP TI   ALSLAMAA+I   LL L +  AA   P+LPPG MGFPF+G+TL  LK
Sbjct: 3   APASFSPLTI---ALSLAMAAVILCQLL-LKLFAAATPSPSLPPGCMGFPFVGETLSFLK 62

Query: 66  PHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMH 125
           PH SNSIGTFL  HCSR+GKVFKSHLFG  AIVSCD+ELNMFILQNDDKLFKVSYPKAMH
Sbjct: 63  PHHSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMH 122

Query: 126 GILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFC 185
           GILG+NSLIIAAGDTHRKLRNVVVSF+ WCKS P+FLHCV  L++SLMDSWRSQ +V FC
Sbjct: 123 GILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFC 182

Query: 186 KEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARER 245
           KEVKMFALS+MVKELLGI+A E+IGS+IL+EFE YMKGFVSLP+NLPGT Y  AVKAR +
Sbjct: 183 KEVKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAK 242

Query: 246 LSCMVKEMMRERKRRGMVVGAD------EDFLQVIMSNWKLNDEEIVSVVLDILLGGYET 305
           LS +VKE+MRERK++G+VVG +      E+FL VIMSNWKL++EEIVSVVLDILLGGYET
Sbjct: 243 LSGIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYET 302

Query: 306 TATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMR 365
           TATLMGLIVYFLAHSPPNVLA LKEEHEAIR  K  GE LNLEDYKQMEFT NV++EAMR
Sbjct: 303 TATLMGLIVYFLAHSPPNVLANLKEEHEAIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMR 362

Query: 366 CGNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEM 425
           CGNVVKFLHREA+KDVKFK+I IPSGWKVHPVFSA+HLDP LHP PQQFNPWRW DDKEM
Sbjct: 363 CGNVVKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEM 422

Query: 426 GKKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 454
            KKVTPFGGGPRLCPG+ELAKLEI+FFLHHLVLNY
Sbjct: 423 SKKVTPFGGGPRLCPGIELAKLEIAFFLHHLVLNY 453

BLAST of MC04g1598 vs. NCBI nr
Match: XP_022990088.1 (cytochrome P450 724B1 [Cucurbita maxima])

HSP 1 Score: 718 bits (1854), Expect = 1.94e-251
Identity = 360/455 (79.12%), Postives = 398/455 (87.47%), Query Frame = 0

Query: 6   AAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLK 65
           A AS SP   L IALSLAMAA+I   LL L +  AA   P+LPPG MGFPF+G+TL  LK
Sbjct: 3   APASFSP---LSIALSLAMAAVILCQLL-LKLFAAATPSPSLPPGCMGFPFVGETLSFLK 62

Query: 66  PHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMH 125
           PH SNSIGTFL +HCSR+G VFKSHLFG PAIVSCD+ELNMFILQNDDKLFKVSYPKAMH
Sbjct: 63  PHHSNSIGTFLQYHCSRFGTVFKSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMH 122

Query: 126 GILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFC 185
           GILG+NSLIIAAGDTHRKLRNVVVSF+ WCKS P+FLH V  L++SLM+SWR Q +VFFC
Sbjct: 123 GILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHSVDKLSVSLMESWRCQTRVFFC 182

Query: 186 KEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARER 245
           KEVKMFALS+MVKELLGIEA E++G +IL+EFE YMKGFVSLP+NLPGT Y  AVKAR +
Sbjct: 183 KEVKMFALSVMVKELLGIEANESVGLRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAK 242

Query: 246 LSCMVKEMMRERKRRGMVVGAD------EDFLQVIMSNWKLNDEEIVSVVLDILLGGYET 305
           LS +VKEMM+ERK++G+VVG +      E+FL VIMSNWKL++EEIVSVVLDILLGGYET
Sbjct: 243 LSGIVKEMMKERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYET 302

Query: 306 TATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMR 365
           TATLMGLIVYFLAHSPPNVLAKLKEEHEAIR  K  GE LNLEDYKQMEFT NV++EAMR
Sbjct: 303 TATLMGLIVYFLAHSPPNVLAKLKEEHEAIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMR 362

Query: 366 CGNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEM 425
           CGNVVKFLHREA+KDVKFK+I IPSGWKVHPVFSA+HLDP LHP PQQFNPWRW DDKEM
Sbjct: 363 CGNVVKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEM 422

Query: 426 GKKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 454
            KKVTPFGGGPRLCPG+ELAKLEI+FFLHHLVLNY
Sbjct: 423 SKKVTPFGGGPRLCPGIELAKLEIAFFLHHLVLNY 453

BLAST of MC04g1598 vs. ExPASy TrEMBL
Match: A0A6J1BWX9 (cytochrome P450 724B1 OS=Momordica charantia OX=3673 GN=LOC111006083 PE=3 SV=1)

HSP 1 Score: 910 bits (2353), Expect = 0.0
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0

Query: 1   MELVAAAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQT 60
           MELVAAAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQT
Sbjct: 1   MELVAAAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQT 60

Query: 61  LGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSY 120
           LGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSY
Sbjct: 61  LGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSY 120

Query: 121 PKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQN 180
           PKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQN
Sbjct: 121 PKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQN 180

Query: 181 QVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAV 240
           QVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAV
Sbjct: 181 QVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAV 240

Query: 241 KARERLSCMVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETT 300
           KARERLSCMVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETT
Sbjct: 241 KARERLSCMVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETT 300

Query: 301 ATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRC 360
           ATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRC
Sbjct: 301 ATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRC 360

Query: 361 GNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEMG 420
           GNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEMG
Sbjct: 361 GNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEMG 420

Query: 421 KKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 454
           KKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY
Sbjct: 421 KKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 454

BLAST of MC04g1598 vs. ExPASy TrEMBL
Match: A0A6J1JS89 (cytochrome P450 724B1 OS=Cucurbita maxima OX=3661 GN=LOC111487092 PE=3 SV=1)

HSP 1 Score: 718 bits (1854), Expect = 9.40e-252
Identity = 360/455 (79.12%), Postives = 398/455 (87.47%), Query Frame = 0

Query: 6   AAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLK 65
           A AS SP   L IALSLAMAA+I   LL L +  AA   P+LPPG MGFPF+G+TL  LK
Sbjct: 3   APASFSP---LSIALSLAMAAVILCQLL-LKLFAAATPSPSLPPGCMGFPFVGETLSFLK 62

Query: 66  PHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMH 125
           PH SNSIGTFL +HCSR+G VFKSHLFG PAIVSCD+ELNMFILQNDDKLFKVSYPKAMH
Sbjct: 63  PHHSNSIGTFLQYHCSRFGTVFKSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMH 122

Query: 126 GILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFC 185
           GILG+NSLIIAAGDTHRKLRNVVVSF+ WCKS P+FLH V  L++SLM+SWR Q +VFFC
Sbjct: 123 GILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHSVDKLSVSLMESWRCQTRVFFC 182

Query: 186 KEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARER 245
           KEVKMFALS+MVKELLGIEA E++G +IL+EFE YMKGFVSLP+NLPGT Y  AVKAR +
Sbjct: 183 KEVKMFALSVMVKELLGIEANESVGLRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAK 242

Query: 246 LSCMVKEMMRERKRRGMVVGAD------EDFLQVIMSNWKLNDEEIVSVVLDILLGGYET 305
           LS +VKEMM+ERK++G+VVG +      E+FL VIMSNWKL++EEIVSVVLDILLGGYET
Sbjct: 243 LSGIVKEMMKERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYET 302

Query: 306 TATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMR 365
           TATLMGLIVYFLAHSPPNVLAKLKEEHEAIR  K  GE LNLEDYKQMEFT NV++EAMR
Sbjct: 303 TATLMGLIVYFLAHSPPNVLAKLKEEHEAIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMR 362

Query: 366 CGNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEM 425
           CGNVVKFLHREA+KDVKFK+I IPSGWKVHPVFSA+HLDP LHP PQQFNPWRW DDKEM
Sbjct: 363 CGNVVKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEM 422

Query: 426 GKKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 454
            KKVTPFGGGPRLCPG+ELAKLEI+FFLHHLVLNY
Sbjct: 423 SKKVTPFGGGPRLCPGIELAKLEIAFFLHHLVLNY 453

BLAST of MC04g1598 vs. ExPASy TrEMBL
Match: A0A6J1E2M5 (cytochrome P450 724B1 OS=Cucurbita moschata OX=3662 GN=LOC111430228 PE=3 SV=1)

HSP 1 Score: 716 bits (1849), Expect = 5.38e-251
Identity = 361/455 (79.34%), Postives = 396/455 (87.03%), Query Frame = 0

Query: 6   AAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLK 65
           A AS SP TI   ALSLAMAA+I   LL L +  AA   P+LPPG MGFPF+G+TL  LK
Sbjct: 3   APASFSPLTI---ALSLAMAAVILCQLL-LKLFAAATPSPSLPPGCMGFPFVGETLSFLK 62

Query: 66  PHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMH 125
           PH SNSIGTFL  HCSR+GKVFKSHLFG  AIVSCD+ELNMFILQNDDKLFKVSYPKAMH
Sbjct: 63  PHHSNSIGTFLQRHCSRFGKVFKSHLFGSWAIVSCDRELNMFILQNDDKLFKVSYPKAMH 122

Query: 126 GILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFC 185
           GILG+NSLIIAAGDTHRKLRNVVVSF+ WCKS P+FLHCV  L++SLMDSWRSQ +V FC
Sbjct: 123 GILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFC 182

Query: 186 KEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARER 245
           KEVKMFALS+MVKELLGIEA E+IGS+IL+EFE YMKGFVSLP+NL GT Y  AVKAR +
Sbjct: 183 KEVKMFALSVMVKELLGIEANESIGSRILDEFETYMKGFVSLPLNLLGTPYYKAVKARAK 242

Query: 246 LSCMVKEMMRERKRRGMVVGAD------EDFLQVIMSNWKLNDEEIVSVVLDILLGGYET 305
           LS +VKEMMRERK++G+VVG +      E+FL VIMSNWKL++EE VSVVLDILLGGYET
Sbjct: 243 LSGIVKEMMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEETVSVVLDILLGGYET 302

Query: 306 TATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMR 365
           TATLMGLIVYFLAHSPP+VLAKLKEEHE IR  K  GE LNLEDYKQMEFT NV++EAMR
Sbjct: 303 TATLMGLIVYFLAHSPPHVLAKLKEEHEGIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMR 362

Query: 366 CGNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEM 425
           CGNVVKFLHREA+KDVKFK+I IPSGWKVHPVFSA+HLDP LHP PQQFNPWRW DDKEM
Sbjct: 363 CGNVVKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEM 422

Query: 426 GKKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 454
            KKVTPFGGGPRLCPG+ELAKLEI+FFLHHLVLNY
Sbjct: 423 SKKVTPFGGGPRLCPGIELAKLEIAFFLHHLVLNY 453

BLAST of MC04g1598 vs. ExPASy TrEMBL
Match: A0A6J1BYG4 (pre-rRNA-processing protein ESF2 OS=Momordica charantia OX=3673 GN=LOC111006727 PE=3 SV=1)

HSP 1 Score: 712 bits (1837), Expect = 7.12e-251
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 456 MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 515
           MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD
Sbjct: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60

Query: 516 GDKVKKKKKGKKSAKQCVEENSIKAEAGEIESKAESSMKEGGPFSDGGGEKDAAFEKKQI 575
           GDKVKKKKKGKKSAKQCVEENSIKAEAGEIESKAESSMKEGGPFSDGGGEKDAAFEKKQI
Sbjct: 61  GDKVKKKKKGKKSAKQCVEENSIKAEAGEIESKAESSMKEGGPFSDGGGEKDAAFEKKQI 120

Query: 576 PLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPP 635
           PLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPP
Sbjct: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPP 180

Query: 636 HMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKV 695
           HMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKV
Sbjct: 181 HMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKV 240

Query: 696 ATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRE 755
           ATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRE
Sbjct: 241 ATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRE 300

Query: 756 RDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVYQKLPKLIRNFPQTQPVADSA 815
           RDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVYQKLPKLIRNFPQTQPVADSA
Sbjct: 301 RDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVYQKLPKLIRNFPQTQPVADSA 360

Query: 816 VENKQRLSKNILAGVFGSS 834
           VENKQRLSKNILAGVFGSS
Sbjct: 361 VENKQRLSKNILAGVFGSS 379

BLAST of MC04g1598 vs. ExPASy TrEMBL
Match: A0A0A0KNY1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G517880 PE=3 SV=1)

HSP 1 Score: 688 bits (1776), Expect = 6.58e-240
Identity = 342/449 (76.17%), Postives = 392/449 (87.31%), Query Frame = 0

Query: 9   SLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLKPHP 68
           +LSP  I+V+ALS A++ II H LLKL ++ + +N PNLPPGSMG PF+G+TL  L PH 
Sbjct: 7   NLSPMIIIVLALSFAIS-IIFHLLLKLFLVTSKKN-PNLPPGSMGLPFVGETLSFLNPHH 66

Query: 69  SNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMHGIL 128
           SNSIGTFL HH SRYGK+FKS LFG PAIVSCD+ELN F+LQNDDKLFKVSYPKAMH IL
Sbjct: 67  SNSIGTFLQHHFSRYGKIFKSRLFGRPAIVSCDRELNYFVLQNDDKLFKVSYPKAMHNIL 126

Query: 129 GTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFCKEV 188
           GTNSL+I+AGDTHRKLR+V+VSFI  CK+RP+FLH +HNL++SL DSWRSQ  V F KE+
Sbjct: 127 GTNSLLISAGDTHRKLRSVIVSFITRCKTRPNFLHSLHNLSVSLTDSWRSQTHVSFIKEM 186

Query: 189 KMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARERLSC 248
           KMFALS+MVKE+ GIEAKE IG+KI EEFE +M GFVSLP+N PGT Y  AVKAR RLS 
Sbjct: 187 KMFALSLMVKEVFGIEAKELIGTKIFEEFETFMIGFVSLPLNFPGTPYFKAVKARGRLST 246

Query: 249 MVKEMMRERKRRGMVVGA--DEDFLQVIMSN-WKLNDEEIVSVVLDILLGGYETTATLMG 308
           +VKEM+RER++RG+V G   D+DFLQV+MSN WKL+DEEIVSVVLDI+LG YETTATL+G
Sbjct: 247 IVKEMIRERRKRGLVGGNNNDDDFLQVLMSNNWKLSDEEIVSVVLDIMLGSYETTATLLG 306

Query: 309 LIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRCGNVVK 368
           LIVYFLAHSPPN+LAKLKEEH+AIR  KR GE LNLEDYK+MEFT NV +EAMRCGNVVK
Sbjct: 307 LIVYFLAHSPPNILAKLKEEHQAIRNGKRKGECLNLEDYKRMEFTFNVAYEAMRCGNVVK 366

Query: 369 FLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEMGKKVTP 428
           FLHREA+ DVKFK+IVIPSGWKVHPVFSA+HLDP LHPNPQQFNP RW+DDKEM KKVTP
Sbjct: 367 FLHREAITDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQQFNPSRWSDDKEMNKKVTP 426

Query: 429 FGGGPRLCPGVELAKLEISFFLHHLVLNY 454
           FGGGPRLCPG+ELAKLEI+FF+HH VLNY
Sbjct: 427 FGGGPRLCPGIELAKLEIAFFVHHFVLNY 453

BLAST of MC04g1598 vs. TAIR 10
Match: AT5G14400.1 (cytochrome P450, family 724, subfamily A, polypeptide 1 )

HSP 1 Score: 393.7 bits (1010), Expect = 3.8e-109
Identity = 201/352 (57.10%), Postives = 255/352 (72.44%), Query Frame = 0

Query: 106 MFILQNDDKLFKVSYPKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCV 165
           MFILQN+ KLF   YPKAMH ILG  SL++A G+ HRKL+NV++SFI   KS+P FLHC 
Sbjct: 1   MFILQNEGKLFTSDYPKAMHDILGKYSLLLATGEIHRKLKNVIISFINLTKSKPDFLHCA 60

Query: 166 HNLTISLMDSWRSQNQVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFV 225
            NL+IS++ SW++  +V F KEVK+F LS+MV +LL I+ ++     +L++F  YMKGF+
Sbjct: 61  ENLSISILKSWKNCREVEFHKEVKIFTLSVMVNQLLSIKPEDPARLYVLQDFLSYMKGFI 120

Query: 226 SLPINLPGTAYSNAVKARERLSCMVKEMMRERKRRGMVVG---ADEDFLQVIMSNWKLND 285
           SLPI LPGT Y+NA+KAR+RLS  V  M++ER+R    +     +EDFL  I+SN  LN 
Sbjct: 121 SLPIPLPGTGYTNAIKARKRLSARVMGMIKEREREEEDMNNAIREEDFLDSIISNEDLNY 180

Query: 286 EEIVSVVLDILLGGYETTATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLE 345
           EE VS+VLDILLGG+ET+AT + L+VYFLA S PN+L KLKEEH AIR +K +GE LN E
Sbjct: 181 EEKVSIVLDILLGGFETSATTLSLVVYFLAKS-PNLLHKLKEEHAAIRAKKGDGELLNWE 240

Query: 346 DYKQMEFTNNVMFEAMRCGNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALH 405
           DY++MEFT  V+ EA+RC                  E VIP GWKV P+F+AVHLDP+LH
Sbjct: 241 DYQKMEFTQCVISEALRC------------------EYVIPKGWKVFPIFTAVHLDPSLH 300

Query: 406 PNPQQFNPWRWTDDKEMGKKVTPFGGGPRLCPGVELAKLEISFFLHHLVLNY 455
            NP +FNP RWTD  +M KK T FGGG R+CPG EL KL+I+FFLHHLVL+Y
Sbjct: 301 ENPFEFNPMRWTDKAKMNKKTTAFGGGVRVCPGGELGKLQIAFFLHHLVLSY 333

BLAST of MC04g1598 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 355.5 bits (911), Expect = 1.1e-97
Identity = 184/449 (40.98%), Postives = 275/449 (61.25%), Query Frame = 0

Query: 46  NLPPGSMGFPFLGQTLGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELN 105
           NLPPG  G+PFLG+T+G LKP+ + ++G F+  H S+YGK+++S+LFG P IVS D  LN
Sbjct: 38  NLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLN 97

Query: 106 MFILQNDDKLFKVSYPKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCV 165
            FILQN+ +LF+ SYP+++ GILG  S+++  GD HR +R++ ++F+   + R   L  V
Sbjct: 98  RFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDV 157

Query: 166 HNLTISLMDSWRSQNQVFFCK-EVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGF 225
              T+ ++DSW+ QN +F  + E K F  ++M K ++ ++  E    ++ +E+  +MKG 
Sbjct: 158 ERHTLFVLDSWQ-QNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGV 217

Query: 226 VSLPINLPGTAYSNAVKARERLSCMVKEMMRERK------------------------RR 285
           VS P+NLPGTAY  A+++R  +   ++  M ERK                          
Sbjct: 218 VSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDH 277

Query: 286 GMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETTATLMGLIVYFLAHSPPNVLA 345
                 D+D L  ++ +  L+ E+I+ ++L +L  G+ET++  + L ++FL  + P  + 
Sbjct: 278 VRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFL-QACPKAVE 337

Query: 346 KLKEEH-EAIRIRKRNGES-LNLEDYKQMEFTNNVMFEAMRCGNVVKFLHREAVKDVKFK 405
           +L+EEH E  R +K  GES LN +DYK+M+FT  V+ E +R GNVV+FLHR+A+KDV++K
Sbjct: 338 ELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRFLHRKALKDVRYK 397

Query: 406 EIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKE------------MGKKVTPF 456
              IPSGWKV PV SAVHLD + +  P  FNPWRW                  G    PF
Sbjct: 398 GYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGSGSFSTWGNNYMPF 457

BLAST of MC04g1598 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.7e-77
Identity = 167/448 (37.28%), Postives = 253/448 (56.47%), Query Frame = 0

Query: 19  ALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLKPHPSNSIGTFLHH 78
           A  L +++I +  LL L   +       LPPGS+G P +G+T  L+  + + +   F+  
Sbjct: 5   AFLLLLSSIAAGFLLLLR--RTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDE 64

Query: 79  HCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMHGILGTNSLIIAAG 138
             +RYG VF +HLFG P I S D E N F+LQN+ KLF+ SYP ++  +LG +SL++  G
Sbjct: 65  RVARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKG 124

Query: 139 DTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFCKEVKMFALSIMVK 198
             H+++ ++ +SF      +   +  +  L    +DSW S  +V   +E K     + VK
Sbjct: 125 SLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSS--RVLLMEEAKKITFELTVK 184

Query: 199 ELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARERLS-CMVKEMMRER 258
           +L+  +  E     + +E+   ++GF SLP+ L  T Y  A++AR +++  +   +M+ R
Sbjct: 185 QLMSFDPGE-WSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRR 244

Query: 259 KRRGMVVGADEDFLQVIM-SNWKLNDEEIVSVVLDILLGGYETTATLMGLIVYFLAHSPP 318
           +         +D L  ++ ++   +DEEIV  ++ +L+ GYETT+T+M L V FL  + P
Sbjct: 245 EEEEEGAERKKDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTET-P 304

Query: 319 NVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRCGNVVKFLHREAVKDVK 378
             LA+LKEEHE IR  K +  SL   DYK M FT  V+ E +R  N++  + R A+ DV+
Sbjct: 305 LALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVE 364

Query: 379 FKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEM---GKKVTPFGGGPRLC 438
            K   IP GWKV   F AVHLDP    + + FNPWRW  +          TPFGGGPRLC
Sbjct: 365 IKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLC 424

Query: 439 PGVELAKLEISFFLHHLVLNYS-MPKEE 461
           PG ELA++ +S FLH LV  +S +P E+
Sbjct: 425 PGYELARVALSVFLHRLVTGFSWVPAEQ 446

BLAST of MC04g1598 vs. TAIR 10
Match: AT3G56510.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 278.1 bits (710), Expect = 2.3e-74
Identity = 146/252 (57.94%), Postives = 193/252 (76.59%), Query Frame = 0

Query: 586 DSIVRENSGEKILESNNGKSDQRIKR-KKRLLKESAKANMCGICYLSRVPPHMDPLKLRQ 645
           D I  E   ++ ++S   K+D++ K+ K++LLKE++KA+  G+CYLSR+PPHMD ++LR 
Sbjct: 11  DGISEEKESKETMKSQ--KADRKKKKLKEKLLKEASKADNRGVCYLSRIPPHMDHVRLRH 70

Query: 646 ILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKVATMLNGEQI 705
           IL+ YGE+ RIYLAPED+  QV RKRAGGFRGQ FSEGWVEF  K VAK+VA MLNGEQI
Sbjct: 71  ILAQYGELGRIYLAPEDSEAQVHRKRAGGFRGQRFSEGWVEFAKKSVAKRVADMLNGEQI 130

Query: 706 GGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRERDFYLSKVD 765
           GG+K+SS YYD+WNIKYL+KFKWDDLTEE AYK AIREQKL + +SAAKRE+DFYLSK++
Sbjct: 131 GGKKKSSVYYDIWNIKYLTKFKWDDLTEEIAYKSAIREQKLNMVLSAAKREKDFYLSKIE 190

Query: 766 KSRALNSIEERLKKKQKLREDSEMSSE--PVVYQKLPKLIRNFPQTQPVADSAVENKQRL 825
           KSRA+  I+ R++KK+K++E+S  ++E  PV     P+ I  F Q + + +   ++K  L
Sbjct: 191 KSRAMTEIDARMEKKRKIQEESGSNAEAAPVF---PPRAIFQFRQKKSIENETSQSKPGL 250

Query: 826 SKNILAGVFGSS 835
           S + LA VFG S
Sbjct: 251 STDFLASVFGGS 257

BLAST of MC04g1598 vs. TAIR 10
Match: AT3G56510.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 278.1 bits (710), Expect = 2.3e-74
Identity = 146/252 (57.94%), Postives = 193/252 (76.59%), Query Frame = 0

Query: 586 DSIVRENSGEKILESNNGKSDQRIKR-KKRLLKESAKANMCGICYLSRVPPHMDPLKLRQ 645
           D I  E   ++ ++S   K+D++ K+ K++LLKE++KA+  G+CYLSR+PPHMD ++LR 
Sbjct: 11  DGISEEKESKETMKSQ--KADRKKKKLKEKLLKEASKADNRGVCYLSRIPPHMDHVRLRH 70

Query: 646 ILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKVATMLNGEQI 705
           IL+ YGE+ RIYLAPED+  QV RKRAGGFRGQ FSEGWVEF  K VAK+VA MLNGEQI
Sbjct: 71  ILAQYGELGRIYLAPEDSEAQVHRKRAGGFRGQRFSEGWVEFAKKSVAKRVADMLNGEQI 130

Query: 706 GGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRERDFYLSKVD 765
           GG+K+SS YYD+WNIKYL+KFKWDDLTEE AYK AIREQKL + +SAAKRE+DFYLSK++
Sbjct: 131 GGKKKSSVYYDIWNIKYLTKFKWDDLTEEIAYKSAIREQKLNMVLSAAKREKDFYLSKIE 190

Query: 766 KSRALNSIEERLKKKQKLREDSEMSSE--PVVYQKLPKLIRNFPQTQPVADSAVENKQRL 825
           KSRA+  I+ R++KK+K++E+S  ++E  PV     P+ I  F Q + + +   ++K  L
Sbjct: 191 KSRAMTEIDARMEKKRKIQEESGSNAEAAPVF---PPRAIFQFRQKKSIENETSQSKPGL 250

Query: 826 SKNILAGVFGSS 835
           S + LA VFG S
Sbjct: 251 STDFLASVFGGS 257