MC04g1584 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g1584
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionribosomal RNA large subunit methyltransferase E-like
LocationMC04: 23473270 .. 23476274 (+)
RNA-Seq ExpressionMC04g1584
SyntenyMC04g1584
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAATAAAAAAATAAATAAAAAGAAGGATGGCCAAATTAATCTAAAATTCTGATTCTGAATCCCCTCGTCGTCGTAAAGGCTCATGCGACGGAAGTTCGAATATAGGCAACGGCGTTGGTCTTTGAAGTTATTTGAAACCCTAGAAGCATTTGGAAGAAAGAAATGAGTGGAGCAGGAACACCCGATTTCTTCTACAGAGAGGCTCAGCGCCTTGGCTATGTCGCACGATCCGCCTTCAAGGTAAAACCTACTTCCCCTCATCGTCTTCTGTTCTTCCTCCTTCGTAACCTTCCGCCCTCACCAATCAAAATCAACTGTGTATGTATTTCGTCTTCTGCTGATAACAGCTGCTTCAGATTCAGAAGCAGTTCAAATTGATAACTTCTGGCTCCTCCGTTTTGGACCTTGGTTGTGCTCCCGGTGCCTGGCTTCAGGTCTCAATATTGCAGCTTTCAAATCCATTCCGCAAACTTTTACTTCTTTATTTATTTGTTTTTGTTGGGAAGTATGATTTACGTCACTTGCTTTCAGGTGGCTTGCCAGAGTCTGGGCCCTCTGAAGAATGGAGGCTCGGTCTTGGGTATTGATCTCAAGGTGATCTGTTATCTCTTACTGGGTTGTCATTCTGCTGAAACTCATTTTTTTTCAGATAGTAATAATGCGTAAAAGAAATGTGGATCTTGCAGAACAGAAGGTAAAAGTTCCTCATGTGCATTGTGACTCAAGGGTTCAAACTGTTTCTGCTGATGTTATGAACCTTCCCAGAAACAAATTGAAGGCACTGTCTCCTAAGGTAACTTAGCTTACTTATCAAAAGTATGAAATGTTTTATAATTTTACTTTTACCTATGCTATAAGCTCAACTGGTCATGTAGTTTCAAATGCATATCCTCACATTTCTTCTGTTGTATTCTCAAGAATTTTTTTTTTCATCTAGTCTCTTCGAATGATGTTACTCATATCACTCAAAAACTCAATTGCCTAATCCGGGTTTTAGCATAAACCATATCAATAGCATTGAATAGAACTGCCAGATTCGTGCAATGGTTTGGTTTTGATGGTCTGATATCTGTAAAAACATAAACATATTATCGGCAATCCTGCCCCCGAGGAGTGCGGAGGGAAGGTGCAGTAATTGAGCAAGAAAATCTTGCAAGTTACAGAATAGAAGTTACATTGTCTATTGATTGGTCTTGCAGGAGAGAGGATTTTCTGTAATACTCTCCGACATGTGCCCCTCAGTATCTGGAATCACCACTAGAGATGCGACTTTATCAGTTGAATTGGGGATGCAAGCAGTTAATTTGGCTATTGGGGAAGCTGCATTAGGCTCTGGAGATGATGTTATGCATGGAAAGGACAGCGTGGATGATTCAACCTCAGTTGTAGATTATGATGGTCTGCTACAAAGAGGAGGCCACCTCGTCGTAAAACTTTTGGAGAGTGAAGATACCCAAGGTAGAGTATTATTTCCACATAAACTTTAATGTAGGATATGTATGTTTGGATTATCCTTACTTTCTTCCTTTATTATTTACCCCAGAATTCAGCCGAATGTTGAAACCGCTGTTCAGAAAGGCATCCTGGTTGAGGCCCAAGGCTACTAGATCGTCGTCAAGAGAGATTTATTTGATATGTCAAAGCTTGCAGGCAAGAAAATAGAATTTCAAAATTTGGGATGAGAACTTAAATCCTCAGCCTACTCCAGAGACCCAAATGTAGCAAGTCAAAAAGGTAGCCTTCAGACTGAAGCAGAGTTCCCTGGCAACATCGAATCGAGCTTTGTAAGCTTGGATCCGATCAAGATTCAAATCACTATGTTGATTGTTGAGTGACGTAGTGGTACCTTTTTGCTATTAATTGTCATAAGAACGCATATTTAACTTATGAGATTTGTTTAGTAGACTTGGCTGCCATTAGATTACTTGTTATTAACTTGTTCTTTTCAGATCTAAATCCATGAATATAGAAGTACGACTTCCATTGAAATTGTGTGAACCAAGAACCAAAAAAGAACACTCTTATTCATATACTTTACAATCAGAATACAAACAATATCATACTCTCTACAAATCAGAGAGAATACTCTTCACTGTTCATTGTTCAACCGCACTGTTACTCTTTGAATAAAATTGTGTGGAAATTCCAGAGCCAGGATCAAACTGCATTTACAAACGAATGAATAAAATTGGCTTCCCTATGTGATGATGTGAGGCATTCATCACCGAATCTGGGTTCGCCCGAGCGACCAAAGGCCCGTATCTCTGACTGGTGTCTGGAAAAACAAAGTTAGCGTTAAATCTTAAGGCAAATTAAGGGAAGGGAAAATCTGAAGAGTATTTTTCTGGAAAGATGATGGTTGTTTTGCTGTCACCCCCTTTTTACCTGTGTTTTCATTGATGGGAAGATATTCCAGAACCGAAGAGTTTCATCTCCAGCACCTGTTACAATTGTCTGCAAGAAGCCATTTATAAGACTCTCAAAGAGGAAATTGCTTATGGGAAAGAGGATATTATGATAATTAATTACACTTGAACAAATACCTGGCCATCGGGTGACATTGCAAGGTACAACACCCTCATCGTGTGTCCAGTTAGAGTAGCAACCTTCCCCATTGATGGATACTTCCACACCATAATCTGGTTCTGGGAATACCCATGCGTGCTCACAAGTTCATTTACGTTTTTACTCCATGCCAGATTGCAAACCTGAGAAAGAATTTGACAAAATTCATCAAGTTTTAGCTGAGGGAAACAGCTCCCATCATATCAGTTCTTTACAAGCAGCATAAGCAATCAAAACCTGGCTTCCAGTGTCGACAGACTCCAACTGGTGGCCATTTGCAGTATTCCAGAATCGAATGCATCGGTCCGCAGTTCCCCCACCGGATGCAAGAAGGCCACTCTGGTGCGGCGACCAGGCGATCGCCTTAACTGCAGCCGTGTGCTCCGTCAGTCTCAAAACAGGCTGCTGCGAATGCTGGTTCCATACCAACAGCTGTTG

mRNA sequence

AAAAATAAAAAAATAAATAAAAAGAAGGATGGCCAAATTAATCTAAAATTCTGATTCTGAATCCCCTCGTCGTCGTAAAGGCTCATGCGACGGAAGTTCGAATATAGGCAACGGCGTTGGTCTTTGAAGTTATTTGAAACCCTAGAAGCATTTGGAAGAAAGAAATGAGTGGAGCAGGAACACCCGATTTCTTCTACAGAGAGGCTCAGCGCCTTGGCTATGTCGCACGATCCGCCTTCAAGCTGCTTCAGATTCAGAAGCAGTTCAAATTGATAACTTCTGGCTCCTCCGTTTTGGACCTTGGTTGTGCTCCCGGTGCCTGGCTTCAGGTGGCTTGCCAGAGTCTGGGCCCTCTGAAGAATGGAGGCTCGGTCTTGGGTATTGATCTCAAGAAGGTAAAAGTTCCTCATGTGCATTGTGACTCAAGGGTTCAAACTGTTTCTGCTGATGTTATGAACCTTCCCAGAAACAAATTGAAGGCACTGTCTCCTAAGGAGAGAGGATTTTCTGTAATACTCTCCGACATGTGCCCCTCAGTATCTGGAATCACCACTAGAGATGCGACTTTATCAGTTGAATTGGGGATGCAAGCAGTTAATTTGGCTATTGGGGAAGCTGCATTAGGCTCTGGAGATGATGTTATGCATGGAAAGGACAGCGTGGATGATTCAACCTCAGTTGTAGATTATGATGGTCTGCTACAAAGAGGAGGCCACCTCGTCGTAAAACTTTTGGAGAGTGAAGATACCCAAGAATTCAGCCGAATGTTGAAACCGCTGTTCAGAAAGGCATCCTGGTTGAGGCCCAAGGCTACTAGATCGTCGTCAAGAGAGATTTATTTGATATGTCAAAGCTTGCAGGCAAGAAAATAGAATTTCAAAATTTGGGATGAGAACTTAAATCCTCAGCCTACTCCAGAGACCCAAATGTAGCAAGTCAAAAAGGTAGCCTTCAGACTGAAGCAGAGTTCCCTGGCAACATCGAATCGAGCTTTGTAAGCTTGGATCCGATCAAGATTCAAATCACTATGTTGATTGTTGAGTGACGTAGTGGTACCTTTTTGCTATTAATTGTCATAAGAACGCATATTTAACTTATGAGATTTGTTTAGTAGACTTGGCTGCCATTAGATTACTTGTTATTAACTTGTTCTTTTCAGATCTAAATCCATGAATATAGAAGTACGACTTCCATTGAAATTGTGTGAACCAAGAACCAAAAAAGAACACTCTTATTCATATACTTTACAATCAGAATACAAACAATATCATACTCTCTACAAATCAGAGAGAATACTCTTCACTGTTCATTGTTCAACCGCACTGTTACTCTTTGAATAAAATTGTGTGGAAATTCCAGAGCCAGGATCAAACTGCATTTACAAACGAATGAATAAAATTGGCTTCCCTATGTGATGATGTGAGGCATTCATCACCGAATCTGGGTTCGCCCGAGCGACCAAAGGCCCGTATCTCTGACTGGTGTCTGGAAAAACAAAGTTAGCGTTAAATCTTAAGGCAAATTAAGGGAAGGGAAAATCTGAAGAGTATTTTTCTGGAAAGATGATGGTTGTTTTGCTGTCACCCCCTTTTTACCTGTGTTTTCATTGATGGGAAGATATTCCAGAACCGAAGAGTTTCATCTCCAGCACCTGTTACAATTGTCTGCAAGAAGCCATTTATAAGACTCTCAAAGAGGAAATTGCTTATGGGAAAGAGGATATTATGATAATTAATTACACTTGAACAAATACCTGGCCATCGGGTGACATTGCAAGGTACAACACCCTCATCGTGTGTCCAGTTAGAGTAGCAACCTTCCCCATTGATGGATACTTCCACACCATAATCTGGTTCTGGGAATACCCATGCGTGCTCACAAGTTCATTTACGTTTTTACTCCATGCCAGATTGCAAACCTGAGAAAGAATTTGACAAAATTCATCAAGTTTTAGCTGAGGGAAACAGCTCCCATCATATCAGTTCTTTACAAGCAGCATAAGCAATCAAAACCTGGCTTCCAGTGTCGACAGACTCCAACTGGTGGCCATTTGCAGTATTCCAGAATCGAATGCATCGGTCCGCAGTTCCCCCACCGGATGCAAGAAGGCCACTCTGGTGCGGCGACCAGGCGATCGCCTTAACTGCAGCCGTGTGCTCCGTCAGTCTCAAAACAGGCTGCTGCGAATGCTGGTTCCATACCAACAGCTGTTG

Coding sequence (CDS)

ATGAGTGGAGCAGGAACACCCGATTTCTTCTACAGAGAGGCTCAGCGCCTTGGCTATGTCGCACGATCCGCCTTCAAGCTGCTTCAGATTCAGAAGCAGTTCAAATTGATAACTTCTGGCTCCTCCGTTTTGGACCTTGGTTGTGCTCCCGGTGCCTGGCTTCAGGTGGCTTGCCAGAGTCTGGGCCCTCTGAAGAATGGAGGCTCGGTCTTGGGTATTGATCTCAAGAAGGTAAAAGTTCCTCATGTGCATTGTGACTCAAGGGTTCAAACTGTTTCTGCTGATGTTATGAACCTTCCCAGAAACAAATTGAAGGCACTGTCTCCTAAGGAGAGAGGATTTTCTGTAATACTCTCCGACATGTGCCCCTCAGTATCTGGAATCACCACTAGAGATGCGACTTTATCAGTTGAATTGGGGATGCAAGCAGTTAATTTGGCTATTGGGGAAGCTGCATTAGGCTCTGGAGATGATGTTATGCATGGAAAGGACAGCGTGGATGATTCAACCTCAGTTGTAGATTATGATGGTCTGCTACAAAGAGGAGGCCACCTCGTCGTAAAACTTTTGGAGAGTGAAGATACCCAAGAATTCAGCCGAATGTTGAAACCGCTGTTCAGAAAGGCATCCTGGTTGAGGCCCAAGGCTACTAGATCGTCGTCAAGAGAGATTTATTTGATATGTCAAAGCTTGCAGGCAAGAAAATAG

Protein sequence

MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQSLGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSDMCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQRGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK
Homology
BLAST of MC04g1584 vs. ExPASy Swiss-Prot
Match: A8ZT18 (Ribosomal RNA large subunit methyltransferase E OS=Desulfococcus oleovorans (strain DSM 6200 / JCM 39069 / Hxd3) OX=96561 GN=rlmE PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.2e-26
Identity = 76/225 (33.78%), Postives = 112/225 (49.78%), Query Frame = 0

Query: 3   GAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQSLG 62
           G  TPD + R+A+   Y ARS +KL +IQK+  ++  G++VLDLGC PG+W+  A +++G
Sbjct: 8   GRKTPDHYARKAKTEHYPARSVYKLQEIQKKHNVLKPGNAVLDLGCFPGSWMMFAAETVG 67

Query: 63  PLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSDMC 122
               GG V GIDLKKV     H  ++  ++  D+  + R   +AL+       V+LSDM 
Sbjct: 68  ---RGGKVTGIDLKKVA---THMPAQATSLQEDIYEIDR---EALAGTLGPLDVVLSDMA 127

Query: 123 PSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQRG 182
           P   G    DA  S  L   A++LA+                             LL+ G
Sbjct: 128 PDTMGNKFTDAARSFHLAAAALDLAL----------------------------FLLKPG 187

Query: 183 GHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLI 228
           GH V K+ + ED Q F  M+K  F +    +P+  R  S+E Y+I
Sbjct: 188 GHFVCKVFQGEDFQNFVNMVKAEFERHKVFKPETCRKDSKETYVI 195

BLAST of MC04g1584 vs. ExPASy Swiss-Prot
Match: B8FL12 (Ribosomal RNA large subunit methyltransferase E OS=Desulfatibacillum aliphaticivorans OX=218208 GN=rlmE PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.9e-24
Identity = 75/220 (34.09%), Postives = 111/220 (50.45%), Query Frame = 0

Query: 8   DFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQSLGPLKNG 67
           D +  +AQ+ G+ ARS FKL ++QK++K++  G  VLDLGCAPG+WL+ A Q +G   + 
Sbjct: 13  DHYTDKAQKAGFPARSVFKLEEMQKRWKILRPGQKVLDLGCAPGSWLKYASQIVG---DS 72

Query: 68  GSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSDMCPSVSG 127
           G V+G+DLK +  P        Q +  D   L +  L  +    R F V+LSDM P+ +G
Sbjct: 73  GRVIGLDLKPMDQPD---KPNAQFIQGDAFELTQEFLDEIG---RDFDVVLSDMAPNTTG 132

Query: 128 ITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQRGGHLVV 187
           I   DA  S          A+ E+AL +   V                   L+ GG  V 
Sbjct: 133 IKNVDALKSA---------ALCESALAAAVTV-------------------LKPGGSFVC 192

Query: 188 KLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLI 228
           K+ + E    F + +K  F K    +P++TR  SREIY++
Sbjct: 193 KIFQGEGFDAFIKDVKKYFTKHKIFKPESTRKQSREIYVV 195

BLAST of MC04g1584 vs. ExPASy Swiss-Prot
Match: B9LSX2 (Ribosomal RNA large subunit methyltransferase E OS=Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34) OX=416348 GN=rlmE PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-23
Identity = 72/224 (32.14%), Postives = 111/224 (49.55%), Query Frame = 0

Query: 8   DFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQSLGPLKNG 67
           D +Y ++++ GY ARSA+KL QI ++  L   G +V+DLG APG WLQVA + +G     
Sbjct: 5   DEYYNKSKQQGYRARSAYKLKQIDEEANLFERGDTVVDLGAAPGGWLQVAAEEVG---ES 64

Query: 68  GSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSDMCPSVSG 127
           G+V+G+DL+++       D  V+T+  D+           +  ERG  V++SDM P+++G
Sbjct: 65  GTVVGVDLQRIDDLD---DHDVETIRGDMTEERTRHYLREAIGERGADVVISDMAPNMTG 124

Query: 128 ITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQRGGHLVV 187
               D   SV L  QA ++A                            + LL  GG  VV
Sbjct: 125 EYALDHARSVHLARQAFDVA----------------------------EELLAPGGDFVV 184

Query: 188 KLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSL 232
           K+ + ED   F   ++  F     + P A+R SS E+YL+ + L
Sbjct: 185 KVFQGEDLDAFREDVRAEFEYLRTVSPPASRDSSSEVYLVAKGL 194

BLAST of MC04g1584 vs. ExPASy Swiss-Prot
Match: A6UUK5 (Ribosomal RNA large subunit methyltransferase E OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=rlmE PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.2e-22
Identity = 67/229 (29.26%), Postives = 119/229 (51.97%), Query Frame = 0

Query: 8   DFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQSLGPLKNG 67
           DF+Y+ A++  Y +R+++KLLQ+ ++F++I  G +V+DLGCAPG WLQ +   +G     
Sbjct: 15  DFYYKLAKQNHYRSRASYKLLQLNEKFEIIYEGDNVVDLGCAPGGWLQASYDLVG---ED 74

Query: 68  GSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPR-NKLKALSPKERGFSVILSDMCPSVS 127
           G V+G+DL+KVK      +  +  +  D+      NK+  L P +    V++ D  P++S
Sbjct: 75  GFVIGVDLQKVKPLR---EENIVAIQGDMTKKETINKIIDLMPSKA--DVVICDASPNIS 134

Query: 128 GITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQRGGHLV 187
           G+   D + S+EL + A                            ++    LL++ G+ V
Sbjct: 135 GVWEVDHSRSIELSLMA----------------------------LIATTHLLRKNGNFV 194

Query: 188 VKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK 236
           VK+ +     ++ ++L   F+K    +PKA+RS S E+Y+I +    +K
Sbjct: 195 VKVFQGSLFDQYVQLLTKYFKKVQTTKPKASRSVSAEVYVIGKKFLGKK 207

BLAST of MC04g1584 vs. ExPASy Swiss-Prot
Match: Q58771 (Ribosomal RNA large subunit methyltransferase E OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=rlmE PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.8e-22
Identity = 69/229 (30.13%), Postives = 116/229 (50.66%), Query Frame = 0

Query: 8   DFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQSLGPLKNG 67
           DF+Y+ A++L Y +R++FKL+Q+ ++F +I  G  VLDLGCAPG W+QVA + +G   + 
Sbjct: 15  DFYYKLAKKLKYRSRASFKLMQLNEKFNVIKPGKIVLDLGCAPGGWMQVAREIVG---DK 74

Query: 68  GSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPK-ERGFSVILSDMCPSVS 127
           G V+GIDL+ VK      D+ V       +    NK++ L P  E+   V++SD  P++S
Sbjct: 75  GFVIGIDLQPVK--PFEYDNVVAIKGDFTLEENLNKIRELIPNDEKKVDVVISDASPNIS 134

Query: 128 GITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQRGGHLV 187
           G    D   S++L   A+ +A                              +L+  G+ V
Sbjct: 135 GYWDIDHARSIDLVTTALQIA----------------------------TEMLKERGNFV 194

Query: 188 VKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK 236
            K+   +   ++  ++K  F K    +P+A+R  S E+Y+I +    +K
Sbjct: 195 AKVFYGDMIDDYVNLVKKYFEKVYITKPQASRKESAEVYVIAKRYTGKK 210

BLAST of MC04g1584 vs. NCBI nr
Match: XP_022134055.1 (uncharacterized protein LOC111006421 isoform X1 [Momordica charantia])

HSP 1 Score: 459 bits (1181), Expect = 8.92e-163
Identity = 235/235 (100.00%), Postives = 235/235 (100.00%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD
Sbjct: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ 180
           MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ
Sbjct: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ 180

Query: 181 RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK 235
           RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK
Sbjct: 181 RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK 235

BLAST of MC04g1584 vs. NCBI nr
Match: XP_022134056.1 (uncharacterized protein LOC111006421 isoform X2 [Momordica charantia])

HSP 1 Score: 449 bits (1154), Expect = 1.05e-158
Identity = 232/235 (98.72%), Postives = 232/235 (98.72%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAGTPDFFYREAQRLGYVARSAFK   IQKQFKLITSGSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGTPDFFYREAQRLGYVARSAFK---IQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD
Sbjct: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ 180
           MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ
Sbjct: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ 180

Query: 181 RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK 235
           RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK
Sbjct: 181 RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK 232

BLAST of MC04g1584 vs. NCBI nr
Match: XP_023543349.1 (uncharacterized protein LOC111802921 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 429 bits (1102), Expect = 1.10e-150
Identity = 218/237 (91.98%), Postives = 228/237 (96.20%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAG PDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGAPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGPLKNGGSV+GIDLKKVKVPHVHCDSRVQTV ADVMNLP+NKLKALSPKE+GFSVILSD
Sbjct: 61  LGPLKNGGSVVGIDLKKVKVPHVHCDSRVQTVCADVMNLPKNKLKALSPKEKGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGK---DSVDDSTSVVDYDG 180
           MCPSVSGITTRDA+LSVELGMQAVNLA+GEAAL  GDDVMHGK   D+VDDS SV DYDG
Sbjct: 121 MCPSVSGITTRDASLSVELGMQAVNLALGEAALSHGDDVMHGKNKEDNVDDSASVSDYDG 180

Query: 181 LLQRGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQAR 234
           +LQ+GGHLV+KLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQA+
Sbjct: 181 VLQKGGHLVIKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQAK 237

BLAST of MC04g1584 vs. NCBI nr
Match: KAG6602386.1 (hypothetical protein SDJN03_07619, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 428 bits (1100), Expect = 2.75e-150
Identity = 218/237 (91.98%), Postives = 227/237 (95.78%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAG PDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGAPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGPLKNGGSV+GIDLKKVKVPHVHCDSRVQTV ADVMNLP+NKLKALSPKE+GFSVILSD
Sbjct: 61  LGPLKNGGSVVGIDLKKVKVPHVHCDSRVQTVCADVMNLPKNKLKALSPKEKGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGK---DSVDDSTSVVDYDG 180
           MCP VSGITTRDA+LSVELGMQAVNLA+GEAAL  GDDVMHGK   D+VDDS SV DYDG
Sbjct: 121 MCPMVSGITTRDASLSVELGMQAVNLALGEAALSHGDDVMHGKNKEDNVDDSASVSDYDG 180

Query: 181 LLQRGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQAR 234
           LLQ+GGHLV+KLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQA+
Sbjct: 181 LLQKGGHLVIKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQAK 237

BLAST of MC04g1584 vs. NCBI nr
Match: XP_022960605.1 (uncharacterized protein LOC111461336 [Cucurbita moschata])

HSP 1 Score: 424 bits (1090), Expect = 9.18e-149
Identity = 216/237 (91.14%), Postives = 226/237 (95.36%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAG PDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGAPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGPLKNGGSV+GIDLKKVKVPHVHCDSRVQTV ADVMNLP+NKLKALSPKE+GFSVILSD
Sbjct: 61  LGPLKNGGSVVGIDLKKVKVPHVHCDSRVQTVCADVMNLPKNKLKALSPKEKGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGK---DSVDDSTSVVDYDG 180
           MCP VSGITTRDA+LSVELGMQAVNLA+GEAAL  GDDVMHGK   D+VDDS  V DYDG
Sbjct: 121 MCPLVSGITTRDASLSVELGMQAVNLALGEAALSHGDDVMHGKNKEDNVDDSALVSDYDG 180

Query: 181 LLQRGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQAR 234
           +LQ+GGHLV+KLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQA+
Sbjct: 181 VLQKGGHLVIKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQAK 237

BLAST of MC04g1584 vs. ExPASy TrEMBL
Match: A0A6J1BWZ2 (uncharacterized protein LOC111006421 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111006421 PE=3 SV=1)

HSP 1 Score: 459 bits (1181), Expect = 4.32e-163
Identity = 235/235 (100.00%), Postives = 235/235 (100.00%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD
Sbjct: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ 180
           MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ
Sbjct: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ 180

Query: 181 RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK 235
           RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK
Sbjct: 181 RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK 235

BLAST of MC04g1584 vs. ExPASy TrEMBL
Match: A0A6J1BWX0 (uncharacterized protein LOC111006421 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111006421 PE=3 SV=1)

HSP 1 Score: 449 bits (1154), Expect = 5.06e-159
Identity = 232/235 (98.72%), Postives = 232/235 (98.72%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAGTPDFFYREAQRLGYVARSAFK   IQKQFKLITSGSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGTPDFFYREAQRLGYVARSAFK---IQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD
Sbjct: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ 180
           MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ
Sbjct: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ 180

Query: 181 RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK 235
           RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK
Sbjct: 181 RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQARK 232

BLAST of MC04g1584 vs. ExPASy TrEMBL
Match: A0A6J1H9J8 (uncharacterized protein LOC111461336 OS=Cucurbita moschata OX=3662 GN=LOC111461336 PE=3 SV=1)

HSP 1 Score: 424 bits (1090), Expect = 4.44e-149
Identity = 216/237 (91.14%), Postives = 226/237 (95.36%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAG PDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGAPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGPLKNGGSV+GIDLKKVKVPHVHCDSRVQTV ADVMNLP+NKLKALSPKE+GFSVILSD
Sbjct: 61  LGPLKNGGSVVGIDLKKVKVPHVHCDSRVQTVCADVMNLPKNKLKALSPKEKGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGK---DSVDDSTSVVDYDG 180
           MCP VSGITTRDA+LSVELGMQAVNLA+GEAAL  GDDVMHGK   D+VDDS  V DYDG
Sbjct: 121 MCPLVSGITTRDASLSVELGMQAVNLALGEAALSHGDDVMHGKNKEDNVDDSALVSDYDG 180

Query: 181 LLQRGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQAR 234
           +LQ+GGHLV+KLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQA+
Sbjct: 181 VLQKGGHLVIKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQAK 237

BLAST of MC04g1584 vs. ExPASy TrEMBL
Match: A0A6J1JHT1 (uncharacterized protein LOC111487116 OS=Cucurbita maxima OX=3661 GN=LOC111487116 PE=3 SV=1)

HSP 1 Score: 417 bits (1071), Expect = 2.92e-146
Identity = 215/238 (90.34%), Postives = 227/238 (95.38%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAG PDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGAPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGPLKNGGSV+GIDLKKVKVPHV+CDSRVQTV ADVMNLP+NKLKALSPKE+GFSVILSD
Sbjct: 61  LGPLKNGGSVVGIDLKKVKVPHVYCDSRVQTVCADVMNLPKNKLKALSPKEKGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGS-GDDVMHGK---DSVDDSTSVVDYD 180
           MCP VSGITTRDA+LSVELGMQAVN+A+GEAAL S GDDVMHGK   D+VDDS  V DYD
Sbjct: 121 MCPLVSGITTRDASLSVELGMQAVNMALGEAALLSHGDDVMHGKNKEDNVDDSALVSDYD 180

Query: 181 GLLQRGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQAR 234
           G+LQ+GGHLV+KLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQA+
Sbjct: 181 GVLQKGGHLVIKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQAK 238

BLAST of MC04g1584 vs. ExPASy TrEMBL
Match: A0A0A0KQZ0 (FtsJ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G517710 PE=3 SV=1)

HSP 1 Score: 404 bits (1038), Expect = 3.01e-141
Identity = 208/233 (89.27%), Postives = 221/233 (94.85%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAG PDFFYREAQRLGYVARSAFKLLQIQ QFKLITSGSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGAPDFFYREAQRLGYVARSAFKLLQIQNQFKLITSGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGP +NGGSVLGID+KKVKVPH+HCDSRVQTVSADVMNLP++KLK+LSPKE+GFSVILSD
Sbjct: 61  LGPPRNGGSVLGIDIKKVKVPHMHCDSRVQTVSADVMNLPKSKLKSLSPKEKGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKD--SVDDSTSVVDYDGL 180
           MCP VSGITTRDA LSVELGMQAVNLA+GEAALG GD V+HGKD  SVDDSTS  D DG+
Sbjct: 121 MCPLVSGITTRDAALSVELGMQAVNLALGEAALGVGD-VIHGKDKDSVDDSTSASDCDGV 180

Query: 181 LQRGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSL 231
           L++GGHLV+KLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSL
Sbjct: 181 LRQGGHLVLKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSL 232

BLAST of MC04g1584 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 322.4 bits (825), Expect = 3.0e-88
Identity = 163/232 (70.26%), Postives = 191/232 (82.33%), Query Frame = 0

Query: 1   MSGAGTPDFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQS 60
           MSGAG PDFFYREAQRLGYVARSAFKLLQIQKQ+KLI  GSSVLDLGCAPGAWLQVACQS
Sbjct: 1   MSGAGVPDFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQS 60

Query: 61  LGPLKNGGSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVILSD 120
           LGPL++GG V+G+D+KKVKVP   CDSRVQT++ADV+N PR K++ LSP++ GFSVILSD
Sbjct: 61  LGPLRSGGIVVGMDIKKVKVP-PQCDSRVQTIAADVLNFPRQKIRELSPQQLGFSVILSD 120

Query: 121 MCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQ 180
           MC SVSGITTRDA LS ELGM+A++LA+G+AA+           + DD        G+L+
Sbjct: 121 MCHSVSGITTRDAALSAELGMRALDLAVGQAAISQ-------SPNDDDGGPNESRPGVLR 180

Query: 181 RGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQSLQ 233
            GGHLV+KLLESED Q+F+R+ KP+F KASWLRPKATR SSREIYLICQ  +
Sbjct: 181 HGGHLVIKLLESEDAQDFARICKPIFNKASWLRPKATRPSSREIYLICQGFR 224

BLAST of MC04g1584 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 87.4 bits (215), Expect = 1.6e-17
Identity = 67/222 (30.18%), Postives = 107/222 (48.20%), Query Frame = 0

Query: 8   DFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQSLGPLKNG 67
           D +YR A+  G+ +R+++KLLQ+  ++ L+ S  +VLDL  APG W+QVA + + P+  G
Sbjct: 11  DKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQVAVEKV-PV--G 70

Query: 68  GSVLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKE--RGFSVILSDMCPSV 127
             VLGIDL  + +P   C +  Q ++       ++K+K +  +     F+++L D  P+V
Sbjct: 71  SLVLGIDLVPI-LPVRGCVTMTQDITRTEC---KSKIKQVMEQHGVSAFNLVLHDGSPNV 130

Query: 128 SGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGLLQRGGHL 187
            G   ++A     L + +V LA                               L R G+L
Sbjct: 131 GGAWAQEAMSQNALVIDSVRLA----------------------------TEFLARNGNL 190

Query: 188 VVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLI 228
           V K+  S D       L  LF K    +P A+RS+S E YL+
Sbjct: 191 VTKVFRSRDYNSVLYCLGRLFEKVEVFKPPASRSASAETYLV 197

BLAST of MC04g1584 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-14
Identity = 60/232 (25.86%), Postives = 104/232 (44.83%), Query Frame = 0

Query: 8   DFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQVACQSLG-PLKN 67
           D +YR+A+  G+ ARSAFKLLQI ++F +      V+DL  APG+W QV  + L  P K+
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRQLYLPAKS 69

Query: 68  GGS--------VLGIDLKKVKVPHVHCDSRVQTVSADVMNLPRNKLKALSPKERGFSVIL 127
                      ++ IDL+    P    +  +Q V  D+ N    ++           +++
Sbjct: 70  SAESKDGDLPLIVAIDLQ----PMAPIEGVIQ-VQGDITNARTAEVVIRHFDGCKADLVV 129

Query: 128 SDMCPSVSGITTRDATLSVELGMQAVNLAIGEAALGSGDDVMHGKDSVDDSTSVVDYDGL 187
            D  P V+G+   D  +  +L +  + +            V H                +
Sbjct: 130 CDGAPDVTGLHDMDEFVQSQLILAGLTI------------VTH----------------I 189

Query: 188 LQRGGHLVVKLLESEDTQEFSRMLKPLFRKASWLRPKATRSSSREIYLICQS 231
           L+ GG  + K+   +DT      LK  F   ++ +PK++R+SS E + +C++
Sbjct: 190 LKEGGKFIAKIFRGKDTSLLYCQLKLFFPTVTFAKPKSSRNSSIEAFAVCEN 208

BLAST of MC04g1584 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 5.8e-07
Identity = 24/48 (50.00%), Postives = 33/48 (68.75%), Query Frame = 0

Query: 8  DFFYREAQRLGYVARSAFKLLQIQKQFKLITSGSSVLDLGCAPGAWLQ 56
          D +YR+A+  G+ ARSAFKLLQI ++F +      V+DL  APG+W Q
Sbjct: 10 DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A8ZT184.2e-2633.78Ribosomal RNA large subunit methyltransferase E OS=Desulfococcus oleovorans (str... [more]
B8FL123.9e-2434.09Ribosomal RNA large subunit methyltransferase E OS=Desulfatibacillum aliphaticiv... [more]
B9LSX21.1e-2332.14Ribosomal RNA large subunit methyltransferase E OS=Halorubrum lacusprofundi (str... [more]
A6UUK52.2e-2229.26Ribosomal RNA large subunit methyltransferase E OS=Methanococcus aeolicus (strai... [more]
Q587712.8e-2230.13Ribosomal RNA large subunit methyltransferase E OS=Methanocaldococcus jannaschii... [more]
Match NameE-valueIdentityDescription
XP_022134055.18.92e-163100.00uncharacterized protein LOC111006421 isoform X1 [Momordica charantia][more]
XP_022134056.11.05e-15898.72uncharacterized protein LOC111006421 isoform X2 [Momordica charantia][more]
XP_023543349.11.10e-15091.98uncharacterized protein LOC111802921 [Cucurbita pepo subsp. pepo][more]
KAG6602386.12.75e-15091.98hypothetical protein SDJN03_07619, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022960605.19.18e-14991.14uncharacterized protein LOC111461336 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1BWZ24.32e-163100.00uncharacterized protein LOC111006421 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1BWX05.06e-15998.72uncharacterized protein LOC111006421 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1H9J84.44e-14991.14uncharacterized protein LOC111461336 OS=Cucurbita moschata OX=3662 GN=LOC1114613... [more]
A0A6J1JHT12.92e-14690.34uncharacterized protein LOC111487116 OS=Cucurbita maxima OX=3661 GN=LOC111487116... [more]
A0A0A0KQZ03.01e-14189.27FtsJ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G517710 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G13830.13.0e-8870.26FtsJ-like methyltransferase family protein [more]
AT4G25730.11.6e-1730.18FtsJ-like methyltransferase family protein [more]
AT5G01230.12.2e-1425.86S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G01230.25.8e-0750.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015507Ribosomal RNA large subunit methyltransferase EPIRSFPIRSF00546123S_rRNA_mtasecoord: 3..235
e-value: 1.8E-47
score: 159.5
IPR015507Ribosomal RNA large subunit methyltransferase EHAMAPMF_01547RNA_methyltr_Ecoord: 3..232
score: 28.796291
IPR002877Ribosomal RNA methyltransferase FtsJ domainPFAMPF01728FtsJcoord: 19..229
e-value: 2.5E-45
score: 154.8
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 15..235
e-value: 1.1E-51
score: 177.3
NoneNo IPR availablePIRSRPIRSR005461-1PIRSR005461-1coord: 7..231
e-value: 4.8E-46
score: 154.9
NoneNo IPR availablePANTHERPTHR10920:SF18RRNA METHYLTRANSFERASE 2, MITOCHONDRIALcoord: 5..233
NoneNo IPR availablePANTHERPTHR10920RIBOSOMAL RNA METHYLTRANSFERASEcoord: 5..233
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 18..230

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g1584.1MC04g1584.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031167 rRNA methylation
biological_process GO:0032259 methylation
biological_process GO:0001510 RNA methylation
molecular_function GO:0008650 rRNA (uridine-2'-O-)-methyltransferase activity
molecular_function GO:0008168 methyltransferase activity