MC04g1460 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g1460 vs. ExPASy Swiss-Prot
Match: Q9SCP2 (Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2)

HSP 1 Score: 603.2 bits (1554), Expect = 2.7e-171
Identity = 303/484 (62.60%), Postives = 362/484 (74.79%), Query Frame = 0

Query: 7   VLFFFFIWVLSSSLPAPTMATFSFN---LTVPHLLPDPEAVAYEVQRTVNDSFSRRQMLS 66
           ++ FF +++L      P M     N   L +PH  PDPE VAY+VQ  VN S +RRQ L 
Sbjct: 8   IVLFFSLFLL-----VPQMVFSMLNRTLLLIPH--PDPELVAYQVQWKVNASITRRQALD 67

Query: 67  IHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTDSSD 126
              +  +  C TGNPIDDCW CD NW  NRQ LADCGIGFG+ A+GGKGGQ Y VTDSSD
Sbjct: 68  TTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLADCGIGFGQYALGGKGGQFYFVTDSSD 127

Query: 127 SDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGGGCI 186
            D VNP+PGTLRY VIQ+EPLWIVF ++M IKLK EL+ NSYKTLDGRGANVHI GGGCI
Sbjct: 128 DDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLKQELIFNSYKTLDGRGANVHIVGGGCI 187

Query: 187 TLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHCSLS 246
           TLQY+SN+IIHNI+IHHC  +G+TN+R++PTH G+R KSDGDGISIF S++IWIDHCSLS
Sbjct: 188 TLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYGFRTKSDGDGISIFGSKDIWIDHCSLS 247

Query: 247 YCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKLVQR 306
            C DGLIDA+MGSTGITISNN+FSHH+EVMLLGH D Y PDS MQVTIAFN FGEKL+QR
Sbjct: 248 RCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGHSDHYEPDSGMQVTIAFNHFGEKLIQR 307

Query: 307 MPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTSAGD 366
           MPRCRRGYIHVVNN+F  WEMYAIGGSG PTINSQGNRY AP +P AKEVTKRV+T  GD
Sbjct: 308 MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPTNPFAKEVTKRVETPDGD 367

Query: 367 WSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGPRES 426
           W  WNWR+EGD++VNGAFFV SG+G    Y KA SV PKSA+ + Q+T ++GV G     
Sbjct: 368 WKGWNWRSEGDILVNGAFFVASGEGAEMRYEKAYSVEPKSASFITQITFHSGVLG---VG 427

Query: 427 GQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLSVHIMVLILVTILAL 486
           G++++     ++ S   SG D   DY   + G G+ N      S SV + +L  ++ L +
Sbjct: 428 GRNNNLGMWTTTGSEGTSGLDSYNDYTDEMSGAGSTNRL----SFSVLVFLLSSISYLVV 477

Query: 487 YTNS 488
           +T+S
Sbjct: 488 FTSS 477

BLAST of MC04g1460 vs. ExPASy Swiss-Prot
Match: Q93Z04 (Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 2.0e-166
Identity = 298/491 (60.69%), Postives = 366/491 (74.54%), Query Frame = 0

Query: 26  ATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQM------LSIHSKGQANSCQTGNPI 85
           AT   NLT+PH  P P++VA  V R+VN+S +RRQ+       S  S   ++SC+TGNPI
Sbjct: 22  ATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSSSSSCRTGNPI 81

Query: 86  DDCWHC-DSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAV 145
           DDCW C D++W  NRQRLADC IGFG   +GGK G+IY+VTDSSD++P NP PGTLRY V
Sbjct: 82  DDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTNPTPGTLRYGV 141

Query: 146 IQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINI 205
           IQ+EPLWIVFS++M I+LK EL++NSYKTLDGRG+ VHITG GC+TLQY+ ++IIHN++I
Sbjct: 142 IQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYVQHIIIHNLHI 201

Query: 206 HHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTG 265
           + C P+              RG+SDGDGISIF S+ IW+DHCS+S+CTDGLIDA+MGST 
Sbjct: 202 YDCKPSAGFE---------KRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMGSTA 261

Query: 266 ITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNN 325
           ITISNNYF+HHDEVMLLGHDD Y+PD+ MQVTIAFN FG+ LVQRMPRCRRGYIHVVNN+
Sbjct: 262 ITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVVNND 321

Query: 326 FRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDT-SAGDWSEWNWRTEGDVMV 385
           F  W+MYAIGGSG PTINSQGNRY AP DP+AKEVTKRVD+   G+WS WNWRTEGD+M 
Sbjct: 322 FTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGDLME 381

Query: 386 NGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGPR----ESGQSSSYPGDE 445
           NGAFFV SG+G+S+MY KA+SV PK+A+LVDQLT NAGVFGGPR    +SG S S  G +
Sbjct: 382 NGAFFVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFGGPRDDQGQSGNSYSPYGGD 441

Query: 446 SSSSSRASGSDGGG-------------------DYFGMIFGGGNVNSAPSPPSLSVHIMV 486
                 + GS GGG                   + F MIFG    + APS P L++ +  
Sbjct: 442 GGGGGSSGGSSGGGMDVMGGTTRGSSSSSGDDSNVFQMIFG----SDAPSRPRLTL-LFS 498

BLAST of MC04g1460 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 9.5e-145
Identity = 239/379 (63.06%), Postives = 295/379 (77.84%), Query Frame = 0

Query: 40  DPEAVAYEVQRTVNDSFSRRQMLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADC 99
           DP+ VA EV + V  S   R   +   K    SC TGNPIDDCW CD NW  NR+RLADC
Sbjct: 88  DPDMVADEVAKLVQMSEQNR---TARRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRLADC 147

Query: 100 GIGFGRDAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYE 159
           GIGFGR+A+GG+ G+ YIVTD +D D VNP+PGTLR+AVIQ+EPLWIVF  DM I+LK E
Sbjct: 148 GIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQE 207

Query: 160 LMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYR 219
           L+MNS+KT+D RG+NVHI  G CIT+Q+I+NVIIH ++IH C PTG+  +R++P+H G+R
Sbjct: 208 LIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWR 267

Query: 220 GKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDD 279
             +DGD +SIF S +IWIDH SLS+C DGL+DA+MGST IT+SNN+F+HH+EVMLLGH D
Sbjct: 268 TMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSD 327

Query: 280 GYSPDSAMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQG 339
            Y+ D  MQVTIA+N FGE LVQRMPRCR GY HVVNN++  WEMYAIGGS  PTINSQG
Sbjct: 328 SYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQG 387

Query: 340 NRYIAPGDPNAKEVTKRVDTSAGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSV 399
           NRY AP D  AKEVTKRV+T A +W +WNWR+EGD+++NGAFF PSG G S  Y +A+S+
Sbjct: 388 NRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSL 447

Query: 400 APKSAALVDQLTLNAGVFG 419
           A K +++VD +T  AG  G
Sbjct: 448 AAKPSSMVDTITSTAGALG 463

BLAST of MC04g1460 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.8e-144
Identity = 238/386 (61.66%), Postives = 296/386 (76.68%), Query Frame = 0

Query: 40  DPEAVAYEVQRTVNDSFSRRQMLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADC 99
           DPE V  EV R +N+S SRR++          SC +GNPIDDCW CD +W+ NR+RLADC
Sbjct: 52  DPELVVEEVHRKINESISRRKLGFF-------SCGSGNPIDDCWRCDKDWEKNRKRLADC 111

Query: 100 GIGFGRDAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYE 159
           GIGFG++A+GG+ G+IY+VTD  + DPVNPRPGTLRYAVIQDEPLWI+F  DMTI+LK E
Sbjct: 112 GIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQDEPLWIIFKRDMTIQLKEE 171

Query: 160 LMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYR 219
           L+MNS+KTLDGRGA+VHI+GG CIT+QY++N+IIH ++IH C   G+T +R +P H GYR
Sbjct: 172 LIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNTYVRDSPEHYGYR 231

Query: 220 GKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDD 279
             SDGDG+SIF   ++W+DHCSLS C DGLIDAI GST ITISNNY +HH++VMLLGH D
Sbjct: 232 TVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLTHHNKVMLLGHSD 291

Query: 280 GYSPDSAMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQG 339
            Y  D  MQVTIAFN FGE LVQRMPRCR GY HVVNN++  WEMYAIGGS  PTINSQG
Sbjct: 292 TYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 351

Query: 340 NRYIAPGDPNAKEVTKRVDTSAGDWSEWNWRTEGDVMVNGAFFVPSGDG--LSNMYVKAT 399
           NR++AP D ++KEVTK  D    +W  WNWR+EGD+++NGAFF  SG G   S+ Y KA+
Sbjct: 352 NRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAFFTYSGAGPAKSSSYSKAS 411

Query: 400 SVAPKSAALVDQLTLNAGVFGGPRES 424
           S+A + ++ V ++T+ +G     R S
Sbjct: 412 SLAARPSSHVGEITIASGALSCKRGS 430

BLAST of MC04g1460 vs. ExPASy Swiss-Prot
Match: Q9SVQ6 (Putative pectate lyase 14 OS=Arabidopsis thaliana OX=3702 GN=At4g13210 PE=3 SV=2)

HSP 1 Score: 511.5 bits (1316), Expect = 1.1e-143
Identity = 236/380 (62.11%), Postives = 291/380 (76.58%), Query Frame = 0

Query: 36  HLLPDPEAVAYEVQRTVNDSFSRRQMLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQR 95
           H + DPE +A  V  ++ +S  RR++      G  +SC TGNPIDDCW CD  W   R+R
Sbjct: 55  HAVKDPEEIAAMVDMSIRNSTYRRKL------GFFSSCSTGNPIDDCWRCDKKWHRRRKR 114

Query: 96  LADCGIGFGRDAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIK 155
           LADC IGFGR+A+GG+ G+ YIVTD SD DPV P+PGTLRYAVIQDEPLWIVF  DM I 
Sbjct: 115 LADCAIGFGRNAVGGRDGRYYIVTDPSDHDPVTPKPGTLRYAVIQDEPLWIVFKRDMVIT 174

Query: 156 LKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNIRATPTH 215
           L  EL+MNS+KT+DGRG NVHI GG C+T+QY++N+IIH INIH C  TG+  +R++ +H
Sbjct: 175 LSQELIMNSFKTIDGRGVNVHIAGGACLTVQYVTNIIIHGINIHDCKRTGNAMVRSSESH 234

Query: 216 VGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLL 275
            G+R  +DGDGISIF S +IWIDH SLS C DGLIDAIMGST ITISNNY +HH+E +LL
Sbjct: 235 YGWRTMADGDGISIFGSSHIWIDHNSLSSCADGLIDAIMGSTAITISNNYLTHHNEAILL 294

Query: 276 GHDDGYSPDSAMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTI 335
           GH D Y+ D  MQVTIA+N FGE L+QRMPRCR GY HVVNN++  WEMYAIGGS  PTI
Sbjct: 295 GHTDSYTRDKMMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTI 354

Query: 336 NSQGNRYIAPGDPNAKEVTKRVDTSAGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVK 395
           NSQGNR++APG+  AKEVTKRV    G+W+ WNWR++GD+M+NGA+F  SG G S  Y +
Sbjct: 355 NSQGNRFLAPGNRFAKEVTKRVGAGKGEWNNWNWRSQGDLMLNGAYFTSSGAGASANYAR 414

Query: 396 ATSVAPKSAALVDQLTLNAG 416
           A+S+A KS++LV  LT ++G
Sbjct: 415 ASSLAAKSSSLVGMLTSSSG 428

BLAST of MC04g1460 vs. NCBI nr
Match: XP_022133447.1 (probable pectate lyase 12 [Momordica charantia])

HSP 1 Score: 1011 bits (2614), Expect = 0.0
Identity = 499/500 (99.80%), Postives = 499/500 (99.80%), Query Frame = 0

Query: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60
           MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ
Sbjct: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60

Query: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120
           MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD
Sbjct: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120

Query: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180
           SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG
Sbjct: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180

Query: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240
           GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC
Sbjct: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240

Query: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300
           SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL
Sbjct: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300

Query: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTS 360
           VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGN YIAPGDPNAKEVTKRVDTS
Sbjct: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNLYIAPGDPNAKEVTKRVDTS 360

Query: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420
           AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP
Sbjct: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420

Query: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLSVHIMVLILVTI 480
           RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLSVHIMVLILVTI
Sbjct: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLSVHIMVLILVTI 480

Query: 481 LALYTNSNYDALLSFTTLLL 500
           LALYTNSNYDALLSFTTLLL
Sbjct: 481 LALYTNSNYDALLSFTTLLL 500

BLAST of MC04g1460 vs. NCBI nr
Match: XP_038884299.1 (probable pectate lyase 12 [Benincasa hispida])

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 443/499 (88.78%), Postives = 475/499 (95.19%), Query Frame = 0

Query: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60
           M ATTG+LFF  IW LSSSLP  T+A  +FNLTVPHLLPDPE VAY+VQR+VNDSFSRRQ
Sbjct: 1   MFATTGILFF--IWFLSSSLPPLTIANLNFNLTVPHLLPDPETVAYQVQRSVNDSFSRRQ 60

Query: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120
           MLSIHSKGQ+NSCQTGNPIDDCW CDSNWQANRQRLADCGIGFGR AMGGKGGQIYIVTD
Sbjct: 61  MLSIHSKGQSNSCQTGNPIDDCWRCDSNWQANRQRLADCGIGFGRAAMGGKGGQIYIVTD 120

Query: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180
           SSDSDPVNPRPGTLRYAV+QDEPLWIVF+ADMTIKLKYELMMNSYKTLDGRGANVHITGG
Sbjct: 121 SSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGG 180

Query: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240
           GCITLQYISN+IIHNINIHHC+PTGHTNIR++PTH+GYRGKSDGDGISIFSSRNIWIDHC
Sbjct: 181 GCITLQYISNIIIHNINIHHCVPTGHTNIRSSPTHIGYRGKSDGDGISIFSSRNIWIDHC 240

Query: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300
           SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDG++PDSAMQVTIAFNLFGEKL
Sbjct: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGFTPDSAMQVTIAFNLFGEKL 300

Query: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTS 360
           VQRMPRCRRGYIHVVNN+FRSWEMYAIGGSG PTINSQGNRYIAPG+PNAKEVTKRVDT+
Sbjct: 301 VQRMPRCRRGYIHVVNNDFRSWEMYAIGGSGNPTINSQGNRYIAPGNPNAKEVTKRVDTN 360

Query: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420
           AGDWSEWNWRTEGD+MVNGAFFVPSG+GLSNMYVKA+S+ PKSAALVDQLTLNAGVFGG 
Sbjct: 361 AGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSLPPKSAALVDQLTLNAGVFGGS 420

Query: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLSV-HIMVLILVT 480
           RES QS+SYPGDES++SS +SGSDGGGDYFGMIFGGG +NSAP PPSLSV ++++L L T
Sbjct: 421 RESRQSNSYPGDESTTSSGSSGSDGGGDYFGMIFGGGGMNSAP-PPSLSVVNVVILTLFT 480

Query: 481 ILALYTNSNYDALLSFTTL 498
           +L LYTNSNYD LLS TTL
Sbjct: 481 VLVLYTNSNYDVLLSLTTL 496

BLAST of MC04g1460 vs. NCBI nr
Match: XP_022939483.1 (probable pectate lyase 12 [Cucurbita moschata])

HSP 1 Score: 903 bits (2333), Expect = 0.0
Identity = 442/496 (89.11%), Postives = 471/496 (94.96%), Query Frame = 0

Query: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60
           M ATTG+LFF  IWVLSSSLP P MATF+FNLTVPHLLPDPE VAY+VQR VNDSFSRRQ
Sbjct: 1   MFATTGILFF--IWVLSSSLPPPAMATFAFNLTVPHLLPDPETVAYQVQRAVNDSFSRRQ 60

Query: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120
           MLS++SK Q++SCQTGNPIDDCW CDSNWQANRQRLADCGIGFGRDAMGGKGGQIY+VTD
Sbjct: 61  MLSVNSKDQSSSCQTGNPIDDCWRCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYVVTD 120

Query: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180
           SSDSDPVNPRPGTLRYAV+QDEPLWIVF+ADMTIKLKYELMMNSYKTLDGRGANVHITGG
Sbjct: 121 SSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGG 180

Query: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240
           GC+TLQYISNVIIHNINIHHCIPTGHTNIR++PTH+GYRGKSDGDGISIFSSRNIWIDHC
Sbjct: 181 GCVTLQYISNVIIHNINIHHCIPTGHTNIRSSPTHIGYRGKSDGDGISIFSSRNIWIDHC 240

Query: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300
           SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL
Sbjct: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300

Query: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTS 360
           VQRMPRCRRGY HVVNN+FRSWEMYAIGGSG PTINSQGNRYIAPG+PNAKEVTKRVDT+
Sbjct: 301 VQRMPRCRRGYFHVVNNDFRSWEMYAIGGSGDPTINSQGNRYIAPGNPNAKEVTKRVDTN 360

Query: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420
           AGDWSEWNWRTEGD+MVNGAFFVPSG+GLSNMYVKA+SVAPKSAALVD+LTLNAGVFGG 
Sbjct: 361 AGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDRLTLNAGVFGGA 420

Query: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGN-VNSAPSPPSLSV-HIMVLILV 480
           RE+ QS SYPGDESSSSS +SGSDGGGDYFGMIFGGG  VNSAP PP LSV ++++L L+
Sbjct: 421 RENRQSYSYPGDESSSSSTSSGSDGGGDYFGMIFGGGGGVNSAPPPPCLSVVNMLMLSLL 480

Query: 481 TILALYTNSNYDALLS 494
           T+L LYTNS   ALLS
Sbjct: 481 TVLVLYTNSYDSALLS 494

BLAST of MC04g1460 vs. NCBI nr
Match: XP_023550310.1 (probable pectate lyase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902 bits (2331), Expect = 0.0
Identity = 441/496 (88.91%), Postives = 471/496 (94.96%), Query Frame = 0

Query: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60
           M AT G+LFF  IWVLSSSLP P MATF+FNLTVPHLLPDPEAVAY+VQR VNDSFSRRQ
Sbjct: 1   MLATIGILFF--IWVLSSSLPPPAMATFTFNLTVPHLLPDPEAVAYQVQRAVNDSFSRRQ 60

Query: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120
           MLS++SK Q++SCQTGNPIDDCW CDSNWQANRQRLADCGIGFGRDAMGGKGGQIY+VTD
Sbjct: 61  MLSVNSKDQSSSCQTGNPIDDCWRCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYVVTD 120

Query: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180
           SSDSDPVNPRPGTLRYAV+QDEPLWIVF+ADMTIKLKYELMMNSYKTLDGRGANVHITGG
Sbjct: 121 SSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGG 180

Query: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240
           GC+TLQYISNVIIHNINIHHCIPTGHTNIR++PTH+GYRGKSDGDGISIFSSRNIWIDHC
Sbjct: 181 GCVTLQYISNVIIHNINIHHCIPTGHTNIRSSPTHIGYRGKSDGDGISIFSSRNIWIDHC 240

Query: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300
           SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL
Sbjct: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300

Query: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTS 360
           VQRMPRCRRGY HVVNN+FRSWEMYAIGGSG PTINSQGNRYIAPG+PNAKEVTKRVDT+
Sbjct: 301 VQRMPRCRRGYFHVVNNDFRSWEMYAIGGSGDPTINSQGNRYIAPGNPNAKEVTKRVDTN 360

Query: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420
           AGDWSEWNWRTEGD+MVNGAFFVPSG+GLSNMYVKA+SVAPKSAALVD+LTLNAGVFGG 
Sbjct: 361 AGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDRLTLNAGVFGGA 420

Query: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGN-VNSAPSPPSLSV-HIMVLILV 480
           RE+ QS SYPGDESSSSS +S SDGGGDYFGMIFGGG  VNSAP PPSLSV ++++L LV
Sbjct: 421 RENRQSYSYPGDESSSSSTSSSSDGGGDYFGMIFGGGGGVNSAPPPPSLSVVNMLILTLV 480

Query: 481 TILALYTNSNYDALLS 494
           T+L +YTN+   ALLS
Sbjct: 481 TVLVVYTNNYESALLS 494

BLAST of MC04g1460 vs. NCBI nr
Match: KAG7016326.1 (putative pectate lyase 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 441/496 (88.91%), Postives = 471/496 (94.96%), Query Frame = 0

Query: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60
           M ATTG+LFF  IWVLSSSLP P MATF+FNLTVPHLLPDPE VAY+VQR VNDSFSRRQ
Sbjct: 1   MFATTGILFF--IWVLSSSLPPPAMATFAFNLTVPHLLPDPETVAYQVQRAVNDSFSRRQ 60

Query: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120
           M+S++SK Q++SCQTGNPIDDCW CDSNWQANRQRLADCGIGFGRDAMGGKGGQIY+VTD
Sbjct: 61  MVSVNSKDQSSSCQTGNPIDDCWRCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYVVTD 120

Query: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180
           SSDSDPVNPRPGTLRYAV+QDEPLWIVF+ADMTIKLKYELMMNSYKTLDGRGANVHITGG
Sbjct: 121 SSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGG 180

Query: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240
           GC+TLQYISNVIIHNINIHHCIPTGHTNIR++PTH+GYRGKSDGDGISIFSSRNIWIDHC
Sbjct: 181 GCVTLQYISNVIIHNINIHHCIPTGHTNIRSSPTHIGYRGKSDGDGISIFSSRNIWIDHC 240

Query: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300
           SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL
Sbjct: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300

Query: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTS 360
           VQRMPRCRRGY HVVNN+FRSWEMYAIGGSG PTINSQGNRYIAPG+PNAKEVTKRVDT+
Sbjct: 301 VQRMPRCRRGYFHVVNNDFRSWEMYAIGGSGDPTINSQGNRYIAPGNPNAKEVTKRVDTN 360

Query: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420
           AGDWSEWNWRTEGD+MVNGAFFVPSG+GLSNMYVKA+SVAPKSAALVD+LTLNAGVFGG 
Sbjct: 361 AGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDRLTLNAGVFGGA 420

Query: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGN-VNSAPSPPSLSV-HIMVLILV 480
           RE+ QS SYPGDESSSSS +SGSDGGGDYFGMIFGGG  VNSAP PP LSV ++++L L+
Sbjct: 421 RENRQSYSYPGDESSSSSTSSGSDGGGDYFGMIFGGGGGVNSAPPPPCLSVVNMLMLSLL 480

Query: 481 TILALYTNSNYDALLS 494
           T+L LYTNS   ALLS
Sbjct: 481 TVLVLYTNSYDSALLS 494

BLAST of MC04g1460 vs. ExPASy TrEMBL
Match: A0A6J1BWP8 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111006021 PE=3 SV=1)

HSP 1 Score: 1011 bits (2614), Expect = 0.0
Identity = 499/500 (99.80%), Postives = 499/500 (99.80%), Query Frame = 0

Query: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60
           MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ
Sbjct: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60

Query: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120
           MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD
Sbjct: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120

Query: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180
           SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG
Sbjct: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180

Query: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240
           GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC
Sbjct: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240

Query: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300
           SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL
Sbjct: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300

Query: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTS 360
           VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGN YIAPGDPNAKEVTKRVDTS
Sbjct: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNLYIAPGDPNAKEVTKRVDTS 360

Query: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420
           AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP
Sbjct: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420

Query: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLSVHIMVLILVTI 480
           RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLSVHIMVLILVTI
Sbjct: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLSVHIMVLILVTI 480

Query: 481 LALYTNSNYDALLSFTTLLL 500
           LALYTNSNYDALLSFTTLLL
Sbjct: 481 LALYTNSNYDALLSFTTLLL 500

BLAST of MC04g1460 vs. ExPASy TrEMBL
Match: A0A6J1FLS3 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111445375 PE=3 SV=1)

HSP 1 Score: 903 bits (2333), Expect = 0.0
Identity = 442/496 (89.11%), Postives = 471/496 (94.96%), Query Frame = 0

Query: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60
           M ATTG+LFF  IWVLSSSLP P MATF+FNLTVPHLLPDPE VAY+VQR VNDSFSRRQ
Sbjct: 1   MFATTGILFF--IWVLSSSLPPPAMATFAFNLTVPHLLPDPETVAYQVQRAVNDSFSRRQ 60

Query: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120
           MLS++SK Q++SCQTGNPIDDCW CDSNWQANRQRLADCGIGFGRDAMGGKGGQIY+VTD
Sbjct: 61  MLSVNSKDQSSSCQTGNPIDDCWRCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYVVTD 120

Query: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180
           SSDSDPVNPRPGTLRYAV+QDEPLWIVF+ADMTIKLKYELMMNSYKTLDGRGANVHITGG
Sbjct: 121 SSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGG 180

Query: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240
           GC+TLQYISNVIIHNINIHHCIPTGHTNIR++PTH+GYRGKSDGDGISIFSSRNIWIDHC
Sbjct: 181 GCVTLQYISNVIIHNINIHHCIPTGHTNIRSSPTHIGYRGKSDGDGISIFSSRNIWIDHC 240

Query: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300
           SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL
Sbjct: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300

Query: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTS 360
           VQRMPRCRRGY HVVNN+FRSWEMYAIGGSG PTINSQGNRYIAPG+PNAKEVTKRVDT+
Sbjct: 301 VQRMPRCRRGYFHVVNNDFRSWEMYAIGGSGDPTINSQGNRYIAPGNPNAKEVTKRVDTN 360

Query: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420
           AGDWSEWNWRTEGD+MVNGAFFVPSG+GLSNMYVKA+SVAPKSAALVD+LTLNAGVFGG 
Sbjct: 361 AGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDRLTLNAGVFGGA 420

Query: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGN-VNSAPSPPSLSV-HIMVLILV 480
           RE+ QS SYPGDESSSSS +SGSDGGGDYFGMIFGGG  VNSAP PP LSV ++++L L+
Sbjct: 421 RENRQSYSYPGDESSSSSTSSGSDGGGDYFGMIFGGGGGVNSAPPPPCLSVVNMLMLSLL 480

Query: 481 TILALYTNSNYDALLS 494
           T+L LYTNS   ALLS
Sbjct: 481 TVLVLYTNSYDSALLS 494

BLAST of MC04g1460 vs. ExPASy TrEMBL
Match: A0A6J1K0T0 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111489138 PE=3 SV=1)

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 435/493 (88.24%), Postives = 467/493 (94.73%), Query Frame = 0

Query: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60
           M ATTG+LFF  IWVLSSSLP P MATF+FNLTVPHLLPDPE VAY+VQR VNDSFSRRQ
Sbjct: 1   MFATTGILFF--IWVLSSSLPPPAMATFTFNLTVPHLLPDPETVAYQVQRAVNDSFSRRQ 60

Query: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120
           MLS++SK Q++SCQTGNPIDDCW CDSNWQANRQRLADCGIGFGRDAMGGKGGQIY+VTD
Sbjct: 61  MLSVNSKDQSSSCQTGNPIDDCWRCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYVVTD 120

Query: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180
           SSDSDPVNPRPGTLRYAV+QDEPLWIVF+ADMTIKLKYELMMNSYKTLDGRGANVHITGG
Sbjct: 121 SSDSDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGG 180

Query: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240
           GC+TLQYISNVIIHNINIHHCI TGHTNIR++PTH+GYRGKSDGDGISIFSSRNIWIDHC
Sbjct: 181 GCVTLQYISNVIIHNINIHHCISTGHTNIRSSPTHIGYRGKSDGDGISIFSSRNIWIDHC 240

Query: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300
           SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL
Sbjct: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300

Query: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTS 360
           VQRMPRCRRGY HVVNN+FRSWEMYAIGGSG PTINSQGNRYIAPG+PNAKEVTKRVDT+
Sbjct: 301 VQRMPRCRRGYFHVVNNDFRSWEMYAIGGSGSPTINSQGNRYIAPGNPNAKEVTKRVDTN 360

Query: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420
           AGDWSEWNWRTEGD+MVNGAFFVPSG+G+SNMYVKA+SVAPKSAALVD+LTLNAGVFGG 
Sbjct: 361 AGDWSEWNWRTEGDIMVNGAFFVPSGEGVSNMYVKASSVAPKSAALVDRLTLNAGVFGGA 420

Query: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGN-VNSAPSPPSLSV-HIMVLILV 480
           RE+ QS SYPGDESSSSS +SGSDGGGDYFGMIFGGG  VN AP PP LSV ++++L L+
Sbjct: 421 RENRQSYSYPGDESSSSSTSSGSDGGGDYFGMIFGGGGGVNIAPPPPCLSVVNMLILTLL 480

Query: 481 TILALYTNSNYDA 491
           T+L +YTN  YD+
Sbjct: 481 TVLVVYTN--YDS 489

BLAST of MC04g1460 vs. ExPASy TrEMBL
Match: A0A6J1H1R2 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111459254 PE=3 SV=1)

HSP 1 Score: 889 bits (2296), Expect = 0.0
Identity = 439/488 (89.96%), Postives = 462/488 (94.67%), Query Frame = 0

Query: 12  FIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQMLSIHSKGQAN 71
           F W LSSSLP+  MA FSFNLT PHLLPDPE VAYEVQR+VNDSFSRR+MLSIHSKGQ+N
Sbjct: 10  FSWFLSSSLPS--MAAFSFNLTDPHLLPDPETVAYEVQRSVNDSFSRREMLSIHSKGQSN 69

Query: 72  SCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTDSSDSDPVNPRP 131
           SCQTGNPIDDCW C SNWQ NRQRLADC IGFGR+AMGGKGGQIYIVTDSSDSDPVNPRP
Sbjct: 70  SCQTGNPIDDCWRCGSNWQDNRQRLADCSIGFGRNAMGGKGGQIYIVTDSSDSDPVNPRP 129

Query: 132 GTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNV 191
           GTLRYAV+QDEPLWIVF+ADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNV
Sbjct: 130 GTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNV 189

Query: 192 IIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLID 251
           IIHNINIHHCIPTGHTN+R++PTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLID
Sbjct: 190 IIHNINIHHCIPTGHTNVRSSPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLID 249

Query: 252 AIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKLVQRMPRCRRGY 311
           AIMGSTGITISNNYFSHHDEVMLLGH+DGY+PDS MQVTIAFNLFGEKLVQRMPRCRRGY
Sbjct: 250 AIMGSTGITISNNYFSHHDEVMLLGHEDGYTPDSGMQVTIAFNLFGEKLVQRMPRCRRGY 309

Query: 312 IHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTSAGDWSEWNWRT 371
           IHVVNN FRSWEMYAIGGSGGPTINSQGNRYIAPG+PNAKEVTKRVDT+AGDWSEWNWRT
Sbjct: 310 IHVVNNEFRSWEMYAIGGSGGPTINSQGNRYIAPGNPNAKEVTKRVDTNAGDWSEWNWRT 369

Query: 372 EGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGPRESGQSSSYPG 431
           EGD+MVNGAFFVPSG+GLSNMYVKA+SVAPKSAALVDQLTLNAGVFGG RES QSSSYPG
Sbjct: 370 EGDIMVNGAFFVPSGEGLSNMYVKASSVAPKSAALVDQLTLNAGVFGGTRESRQSSSYPG 429

Query: 432 DESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLSV-HIMVLILVTILALYTNSNYD 491
           DES S+S +SGSDGGGDYFGMIFGGG VNSA  PPSLSV +++VL L+TIL LYTNS YD
Sbjct: 430 DESFSNSGSSGSDGGGDYFGMIFGGGGVNSA-LPPSLSVVNMVVLTLLTILVLYTNSIYD 489

Query: 492 ALLSFTTL 498
           ALLS T L
Sbjct: 490 ALLSLTAL 494

BLAST of MC04g1460 vs. ExPASy TrEMBL
Match: A0A5D3BPB8 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00090 PE=3 SV=1)

HSP 1 Score: 887 bits (2293), Expect = 0.0
Identity = 435/494 (88.06%), Postives = 468/494 (94.74%), Query Frame = 0

Query: 1   MSATTGVLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQ 60
           M ATTGVLFF  IW LS +L   TMATF+FNLTV +LLPDPE VAY+VQR+VNDSF RRQ
Sbjct: 1   MFATTGVLFF--IWFLSCTLLPLTMATFNFNLTVQNLLPDPETVAYQVQRSVNDSFFRRQ 60

Query: 61  MLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 120
           MLS+HSKGQ++SCQTGNPIDDCW CDSNWQANRQRLADCGIGFGRDAMGGKGGQIY+VTD
Sbjct: 61  MLSVHSKGQSSSCQTGNPIDDCWRCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYVVTD 120

Query: 121 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 180
           SSD DPVNPRPGTLRYAV+QDEPLWIVF+ADMTIKLKYELMMNSYKTLDGRGANVHITGG
Sbjct: 121 SSDPDPVNPRPGTLRYAVVQDEPLWIVFAADMTIKLKYELMMNSYKTLDGRGANVHITGG 180

Query: 181 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 240
           GCITLQYISNVIIHNINIHHC+PTGHTNIR++PTHVGYRGKSDGDGISIFSSRNIWIDHC
Sbjct: 181 GCITLQYISNVIIHNINIHHCVPTGHTNIRSSPTHVGYRGKSDGDGISIFSSRNIWIDHC 240

Query: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 300
           SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDG++PDS MQVTIAFNLFGEKL
Sbjct: 241 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGFTPDSGMQVTIAFNLFGEKL 300

Query: 301 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTS 360
           VQRMPRCRRGYIHVVNN+FRSWEMYAIGGSG PTINSQGNRYIAPG+PNAKEVTKRVDT+
Sbjct: 301 VQRMPRCRRGYIHVVNNDFRSWEMYAIGGSGNPTINSQGNRYIAPGNPNAKEVTKRVDTN 360

Query: 361 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 420
           AGDWSEWNWRTEGD+MVNGAFFVPSG+GLSNMYVKA+S+ PKSAALVDQLTLNAGVFGG 
Sbjct: 361 AGDWSEWNWRTEGDIMVNGAFFVPSGEGLSNMYVKASSLPPKSAALVDQLTLNAGVFGGS 420

Query: 421 RESGQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLS-VHIMVLILVT 480
           RE+ QSSSYPGDE+SSSS +SGSDGGGDYFGMIFGGG +N+AP PPSLS V++++L L T
Sbjct: 421 RETRQSSSYPGDETSSSSGSSGSDGGGDYFGMIFGGGGINNAP-PPSLSAVNMVILTLFT 480

Query: 481 ILALYTNSNYDALL 493
           +L LYTN NYDALL
Sbjct: 481 VLILYTN-NYDALL 490

BLAST of MC04g1460 vs. TAIR 10
Match: AT5G04310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 608.2 bits (1567), Expect = 5.9e-174
Identity = 302/500 (60.40%), Postives = 368/500 (73.60%), Query Frame = 0

Query: 7   VLFFFFIWVLSSSLPAPTMATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQMLSIH- 66
           +++F  +  LS    A + +   FNL++PH  P PE V   VQR +NDS SRRQ+L+   
Sbjct: 19  IIWFCLLVSLSHHGRASSTSASIFNLSLPHQHPFPEHVVLNVQRKLNDSLSRRQLLTYQQ 78

Query: 67  -----SKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTD 126
                +     SC TGNPIDDCW CD NW ANRQRLADC IGFG+  +GGKGGQ Y+VTD
Sbjct: 79  DDGTTASSPIPSCITGNPIDDCWRCDPNWSANRQRLADCSIGFGQGTLGGKGGQFYLVTD 138

Query: 127 SSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGG 186
           SSD+D  NP PGTLR+AVIQ EPLWI+FS+DM IKLK+EL++ SYKT+DGRG N+ ITG 
Sbjct: 139 SSDNDAANPIPGTLRHAVIQPEPLWIIFSSDMGIKLKHELIIGSYKTIDGRGTNIQITGH 198

Query: 187 GCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHC 246
           GC+T+Q +S+VIIHN++IHHC P+G+T + ++PTHVG+RG SDGDGIS+ +S +IW+DHC
Sbjct: 199 GCLTIQQVSHVIIHNVHIHHCKPSGNTLVASSPTHVGFRGVSDGDGISVSASHHIWVDHC 258

Query: 247 SLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKL 306
           SL YC DGLID I+ ST +TISNNYFSHHDEVMLLGHDD Y+ D  MQVTIAFN FGE L
Sbjct: 259 SLGYCADGLIDVILASTAVTISNNYFSHHDEVMLLGHDDRYTADKGMQVTIAFNHFGEGL 318

Query: 307 VQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTS 366
           VQRMPRCR GYIHVVNN+F +WEMYAIGGS  PTINSQGNRY AP DPNAKEVTKRVD++
Sbjct: 319 VQRMPRCRHGYIHVVNNDFTAWEMYAIGGSASPTINSQGNRYTAPIDPNAKEVTKRVDSN 378

Query: 367 AGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGP 426
              WS WNWRTEGDVMVNGAFFVPSGDG+S  Y +ATSV PK+AA++DQLT+NAGVFG P
Sbjct: 379 EKHWSGWNWRTEGDVMVNGAFFVPSGDGVSPAYARATSVQPKAAAIIDQLTVNAGVFGDP 438

Query: 427 R-ESGQSSSYPG------------DESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPS 486
              +GQ  S+PG             +S  +   SGSD     F +IFG  +   A  P  
Sbjct: 439 SGRNGQGGSFPGITNGGGTITRGYSKSGPAGGGSGSDSDDGLFTLIFGNNSGAVALRPGQ 498

Query: 487 LSVHIMVLILVTILALYTNS 488
           +   ++++IL   +  +T S
Sbjct: 499 VWSILLIIILYWYIPHHTRS 518

BLAST of MC04g1460 vs. TAIR 10
Match: AT3G53190.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 603.2 bits (1554), Expect = 1.9e-172
Identity = 303/484 (62.60%), Postives = 362/484 (74.79%), Query Frame = 0

Query: 7   VLFFFFIWVLSSSLPAPTMATFSFN---LTVPHLLPDPEAVAYEVQRTVNDSFSRRQMLS 66
           ++ FF +++L      P M     N   L +PH  PDPE VAY+VQ  VN S +RRQ L 
Sbjct: 8   IVLFFSLFLL-----VPQMVFSMLNRTLLLIPH--PDPELVAYQVQWKVNASITRRQALD 67

Query: 67  IHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTDSSD 126
              +  +  C TGNPIDDCW CD NW  NRQ LADCGIGFG+ A+GGKGGQ Y VTDSSD
Sbjct: 68  TTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLADCGIGFGQYALGGKGGQFYFVTDSSD 127

Query: 127 SDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGGGCI 186
            D VNP+PGTLRY VIQ+EPLWIVF ++M IKLK EL+ NSYKTLDGRGANVHI GGGCI
Sbjct: 128 DDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLKQELIFNSYKTLDGRGANVHIVGGGCI 187

Query: 187 TLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHCSLS 246
           TLQY+SN+IIHNI+IHHC  +G+TN+R++PTH G+R KSDGDGISIF S++IWIDHCSLS
Sbjct: 188 TLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYGFRTKSDGDGISIFGSKDIWIDHCSLS 247

Query: 247 YCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKLVQR 306
            C DGLIDA+MGSTGITISNN+FSHH+EVMLLGH D Y PDS MQVTIAFN FGEKL+QR
Sbjct: 248 RCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGHSDHYEPDSGMQVTIAFNHFGEKLIQR 307

Query: 307 MPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDTSAGD 366
           MPRCRRGYIHVVNN+F  WEMYAIGGSG PTINSQGNRY AP +P AKEVTKRV+T  GD
Sbjct: 308 MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPTNPFAKEVTKRVETPDGD 367

Query: 367 WSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGPRES 426
           W  WNWR+EGD++VNGAFFV SG+G    Y KA SV PKSA+ + Q+T ++GV G     
Sbjct: 368 WKGWNWRSEGDILVNGAFFVASGEGAEMRYEKAYSVEPKSASFITQITFHSGVLG---VG 427

Query: 427 GQSSSYPGDESSSSSRASGSDGGGDYFGMIFGGGNVNSAPSPPSLSVHIMVLILVTILAL 486
           G++++     ++ S   SG D   DY   + G G+ N      S SV + +L  ++ L +
Sbjct: 428 GRNNNLGMWTTTGSEGTSGLDSYNDYTDEMSGAGSTNRL----SFSVLVFLLSSISYLVV 477

Query: 487 YTNS 488
           +T+S
Sbjct: 488 FTSS 477

BLAST of MC04g1460 vs. TAIR 10
Match: AT3G54920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 587.0 bits (1512), Expect = 1.4e-167
Identity = 298/491 (60.69%), Postives = 366/491 (74.54%), Query Frame = 0

Query: 26  ATFSFNLTVPHLLPDPEAVAYEVQRTVNDSFSRRQM------LSIHSKGQANSCQTGNPI 85
           AT   NLT+PH  P P++VA  V R+VN+S +RRQ+       S  S   ++SC+TGNPI
Sbjct: 22  ATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSSSSSCRTGNPI 81

Query: 86  DDCWHC-DSNWQANRQRLADCGIGFGRDAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAV 145
           DDCW C D++W  NRQRLADC IGFG   +GGK G+IY+VTDSSD++P NP PGTLRY V
Sbjct: 82  DDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTNPTPGTLRYGV 141

Query: 146 IQDEPLWIVFSADMTIKLKYELMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINI 205
           IQ+EPLWIVFS++M I+LK EL++NSYKTLDGRG+ VHITG GC+TLQY+ ++IIHN++I
Sbjct: 142 IQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYVQHIIIHNLHI 201

Query: 206 HHCIPTGHTNIRATPTHVGYRGKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTG 265
           + C P+              RG+SDGDGISIF S+ IW+DHCS+S+CTDGLIDA+MGST 
Sbjct: 202 YDCKPSAGFE---------KRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMGSTA 261

Query: 266 ITISNNYFSHHDEVMLLGHDDGYSPDSAMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNN 325
           ITISNNYF+HHDEVMLLGHDD Y+PD+ MQVTIAFN FG+ LVQRMPRCRRGYIHVVNN+
Sbjct: 262 ITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVVNND 321

Query: 326 FRSWEMYAIGGSGGPTINSQGNRYIAPGDPNAKEVTKRVDT-SAGDWSEWNWRTEGDVMV 385
           F  W+MYAIGGSG PTINSQGNRY AP DP+AKEVTKRVD+   G+WS WNWRTEGD+M 
Sbjct: 322 FTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGDLME 381

Query: 386 NGAFFVPSGDGLSNMYVKATSVAPKSAALVDQLTLNAGVFGGPR----ESGQSSSYPGDE 445
           NGAFFV SG+G+S+MY KA+SV PK+A+LVDQLT NAGVFGGPR    +SG S S  G +
Sbjct: 382 NGAFFVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFGGPRDDQGQSGNSYSPYGGD 441

Query: 446 SSSSSRASGSDGGG-------------------DYFGMIFGGGNVNSAPSPPSLSVHIMV 486
                 + GS GGG                   + F MIFG    + APS P L++ +  
Sbjct: 442 GGGGGSSGGSSGGGMDVMGGTTRGSSSSSGDDSNVFQMIFG----SDAPSRPRLTL-LFS 498

BLAST of MC04g1460 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 515.0 bits (1325), Expect = 6.8e-146
Identity = 239/379 (63.06%), Postives = 295/379 (77.84%), Query Frame = 0

Query: 40  DPEAVAYEVQRTVNDSFSRRQMLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADC 99
           DP+ VA EV + V  S   R   +   K    SC TGNPIDDCW CD NW  NR+RLADC
Sbjct: 88  DPDMVADEVAKLVQMSEQNR---TARRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRLADC 147

Query: 100 GIGFGRDAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYE 159
           GIGFGR+A+GG+ G+ YIVTD +D D VNP+PGTLR+AVIQ+EPLWIVF  DM I+LK E
Sbjct: 148 GIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQE 207

Query: 160 LMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYR 219
           L+MNS+KT+D RG+NVHI  G CIT+Q+I+NVIIH ++IH C PTG+  +R++P+H G+R
Sbjct: 208 LIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWR 267

Query: 220 GKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDD 279
             +DGD +SIF S +IWIDH SLS+C DGL+DA+MGST IT+SNN+F+HH+EVMLLGH D
Sbjct: 268 TMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSD 327

Query: 280 GYSPDSAMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQG 339
            Y+ D  MQVTIA+N FGE LVQRMPRCR GY HVVNN++  WEMYAIGGS  PTINSQG
Sbjct: 328 SYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQG 387

Query: 340 NRYIAPGDPNAKEVTKRVDTSAGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSV 399
           NRY AP D  AKEVTKRV+T A +W +WNWR+EGD+++NGAFF PSG G S  Y +A+S+
Sbjct: 388 NRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSL 447

Query: 400 APKSAALVDQLTLNAGVFG 419
           A K +++VD +T  AG  G
Sbjct: 448 AAKPSSMVDTITSTAGALG 463

BLAST of MC04g1460 vs. TAIR 10
Match: AT4G13710.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 515.0 bits (1325), Expect = 6.8e-146
Identity = 239/379 (63.06%), Postives = 295/379 (77.84%), Query Frame = 0

Query: 40  DPEAVAYEVQRTVNDSFSRRQMLSIHSKGQANSCQTGNPIDDCWHCDSNWQANRQRLADC 99
           DP+ VA EV + V  S   R   +   K    SC TGNPIDDCW CD NW  NR+RLADC
Sbjct: 69  DPDMVADEVAKLVQMSEQNR---TARRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRLADC 128

Query: 100 GIGFGRDAMGGKGGQIYIVTDSSDSDPVNPRPGTLRYAVIQDEPLWIVFSADMTIKLKYE 159
           GIGFGR+A+GG+ G+ YIVTD +D D VNP+PGTLR+AVIQ+EPLWIVF  DM I+LK E
Sbjct: 129 GIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQE 188

Query: 160 LMMNSYKTLDGRGANVHITGGGCITLQYISNVIIHNINIHHCIPTGHTNIRATPTHVGYR 219
           L+MNS+KT+D RG+NVHI  G CIT+Q+I+NVIIH ++IH C PTG+  +R++P+H G+R
Sbjct: 189 LIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWR 248

Query: 220 GKSDGDGISIFSSRNIWIDHCSLSYCTDGLIDAIMGSTGITISNNYFSHHDEVMLLGHDD 279
             +DGD +SIF S +IWIDH SLS+C DGL+DA+MGST IT+SNN+F+HH+EVMLLGH D
Sbjct: 249 TMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSD 308

Query: 280 GYSPDSAMQVTIAFNLFGEKLVQRMPRCRRGYIHVVNNNFRSWEMYAIGGSGGPTINSQG 339
            Y+ D  MQVTIA+N FGE LVQRMPRCR GY HVVNN++  WEMYAIGGS  PTINSQG
Sbjct: 309 SYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQG 368

Query: 340 NRYIAPGDPNAKEVTKRVDTSAGDWSEWNWRTEGDVMVNGAFFVPSGDGLSNMYVKATSV 399
           NRY AP D  AKEVTKRV+T A +W +WNWR+EGD+++NGAFF PSG G S  Y +A+S+
Sbjct: 369 NRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSL 428

Query: 400 APKSAALVDQLTLNAGVFG 419
           A K +++VD +T  AG  G
Sbjct: 429 AAKPSSMVDTITSTAGALG 444