MC04g1459 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g1459 vs. ExPASy Swiss-Prot
Match: Q0WTB8 (SHUGOSHIN 2 OS=Arabidopsis thaliana OX=3702 GN=SGO2 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 9.5e-34
Identity = 156/499 (31.26%), Postives = 233/499 (46.69%), Query Frame = 0

Query: 6   MKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLM-TYECVDKLQKEN 65
           M +T   +  S++V GSQ+       N+K+   + K +T+Q+ ++L  + E   KLQKEN
Sbjct: 1   MVLTEMSLDYSNRVLGSQK------DNVKQ---MDKEETQQKENMLFSSQEYAAKLQKEN 60

Query: 66  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 125
           MTLMK +A RN+++E+SG E++KLR N + +Q++NLQLAQ NSQMLAELN+ +DRLK LQ
Sbjct: 61  MTLMKALAHRNKLVELSGIEIQKLRINLRSVQEKNLQLAQANSQMLAELNTNRDRLKDLQ 120

Query: 126 HELGCKNGILLSRK-LDQEI----KEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKAN 185
           HELGCKN +L  +K L++++      +S    +      +C   +     +KDT+  +  
Sbjct: 121 HELGCKNALLKVKKHLEEQVLPRTHHESKDKVSASASDGDCKSFQVHDIKHKDTKRKRTT 180

Query: 186 RPRQS-------------------KRESSG---TSVLQTEVQQVEDKRPCVTRQTARFKT 245
           R + S                   KR  SG   T+ +  E+ Q ED              
Sbjct: 181 RIKSSVSADVKPIPVNDSNSKANRKRRVSGVIDTTGIPEEICQTED------------DI 240

Query: 246 EEPVASTKELSEIENFNSTDFSQCKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATK 305
           ++ V S     +I+N  +  F       V ++       KR C RRQS+RF ++E   T+
Sbjct: 241 DKGVVSRGVNQDIDNVVNKKFVPDAANPVKES----VHRKRQCTRRQSTRFDVQETKQTE 300

Query: 306 DLFEIENSHFIDASQCKETSVIQ----------------------TEVQKVEDKRPCLRR 365
            L E++      A + KET+                          EV++   +R    R
Sbjct: 301 KLLEMDG-----AKESKETASFSLRRRSARLRHEEAEPCKSLHEGDEVRETIKRRRVSLR 360

Query: 366 QSARFKTEEP----VAKTEDLVS------------------------------------S 407
            SARF  +EP     +  +D  S                                     
Sbjct: 361 LSARFDIQEPHVTETSNADDARSIVIEESAGSRSESVEPSESRHETKEITRKRSFSTRRQ 420

BLAST of MC04g1459 vs. ExPASy Swiss-Prot
Match: F4J3S1 (SHUGOSHIN 1 OS=Arabidopsis thaliana OX=3702 GN=SGO1 PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.8e-24
Identity = 127/423 (30.02%), Postives = 206/423 (48.70%), Query Frame = 0

Query: 7   KITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTK--QQSSLLMTYECVDKLQKEN 66
           K  GEKM+    +  +QR++L DI+NL+ Q  L  +  K  QQ+ L+ + E  + LQKEN
Sbjct: 21  KAKGEKMVLEPPMNSAQRRKLGDITNLQNQKNLMNQGAKHQQQAILISSKENAENLQKEN 80

Query: 67  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 126
             LMK++ ER+ I     ++L+KLR  FQK+Q+QNL LAQ N+++LAE N+ KD+LK LQ
Sbjct: 81  TKLMKVVMERDGI----KSDLKKLRIEFQKVQEQNLLLAQANTRILAEFNTSKDQLKVLQ 140

Query: 127 HELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAEC--NEAEESMHTNKDTRPCKANRPR 186
           HELGCKNG++++RK+   +KE+          T  C  + ++     N     CK  +P 
Sbjct: 141 HELGCKNGLVMARKM--LLKEQ----------TLPCTRHASKVKAQANACGGACKTFQPN 200

Query: 187 QSKRE----SSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIE-NFNSTD 246
            +  E    SS  + LQ   +    +R    +  A  +  + +  + E  ++E N ++  
Sbjct: 201 DADHEHASGSSNANSLQRNEKANSKRRVSGRKNPANSEVLDIIGRSGETCQMEDNIDNKK 260

Query: 247 FSQCKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFI--DA--SQC 306
                +           +SKR C  RQSS  K  E   T+ L ++ ++  I  DA  S  
Sbjct: 261 LVSDSDNDAENHINDNVQSKRYCAGRQSSSSKTREASQTETLQKVVDAKEIKGDARFSLT 320

Query: 307 KETSVIQTE----VQKVEDKRPCLRRQSARFKTEEPVAKT--EDLVSSSTSASSLYKEVV 366
           K +  ++++     + + + R  LRR+SAR K++EP       D + ++    S  +  +
Sbjct: 321 KHSDWLKSQEPEPSESLYESRFPLRRRSARLKSQEPEPSESFHDSIETTKRRRSAIRSAM 380

Query: 367 CEIAPTAV--SSVGREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEI--PINVKM 407
             I    V  +  G  DD     K       +S+  +P    A +    KEI     + +
Sbjct: 381 FNIQELGVIQNLNGLPDDQEIAAKARCSAREQSTGSKP---EAVEPHDTKEIIGKSRISL 424

BLAST of MC04g1459 vs. ExPASy Swiss-Prot
Match: E3VXF2 (Shugoshin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=SGO1 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.5e-18
Identity = 118/450 (26.22%), Postives = 186/450 (41.33%), Query Frame = 0

Query: 62  KENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLK 121
           KEN  L  L+AERN++IE+S  EL+K+R   Q +QQ+NLQL Q NSQM AE+N GKDR+K
Sbjct: 55  KENAKLRHLLAERNKVIEVSRVELQKIRLALQAMQQKNLQLVQANSQMFAEINQGKDRIK 114

Query: 122 ALQHELGCKNGILLSRKLDQE-------------------------------------IK 181
            LQHEL C   +L  +  + E                                     I+
Sbjct: 115 LLQHELACTIAVLKVKGSELEKMSKTSNNQQNRAKILEKKTRSSKCAPTKAHQMAAGSIR 174

Query: 182 EKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANRPRQSKRESSGTSVLQTEVQ----- 241
           E    +Q+       C+E  +    +K  + C   R  +S   +  T+ +Q   +     
Sbjct: 175 EHLVEIQSAVPSYTSCHEPPQ----DKTNKRCTNRRKSESCEVTMDTNTVQHSCRPHVEY 234

Query: 242 ----QVEDKRPCVTRQTAR-----FKTEEPVASTKELSEIENFNSTDFSQCKETS----- 301
                 +D R    R++AR     F+  E      E + + +  S++  + +E +     
Sbjct: 235 NGSSHDDDPRKTRRRRSARLNPGSFEVAEICDKLHEDATVPSAPSSNVPKLQEPNAGKDM 294

Query: 302 VVQTEVQKAESKRPCNR----------------------RQSSRFKIEEPVATKDLFEIE 361
           +   +++  + + PC+                        ++ +F IE+P   +    I+
Sbjct: 295 ICGGKMKSLQKELPCDAIAQVVEAPELKEIQEAGSSVAGGEAHKFDIEDPEPPRKSMRID 354

Query: 362 -NSHFIDASQ--------------CKETSVIQTEVQKVEDKRPCLRRQSARFKTE--EPV 406
            N   +++ +              C  TS +  +    E KR   RR+S+R      E  
Sbjct: 355 ANKRKLESFESRLASNKEDCINAICDSTSSVPIQ---HEQKRKLSRRKSSRLDPGPWEVT 414

BLAST of MC04g1459 vs. ExPASy Swiss-Prot
Match: Q4QSC8 (Shugoshin-1 OS=Zea mays OX=4577 GN=SGO1 PE=2 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 2.8e-17
Identity = 132/469 (28.14%), Postives = 211/469 (44.99%), Query Frame = 0

Query: 10  GEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKLQKENMTLMK 69
           G   I+S    G++   L+DI+N   +P   +  T            V  L KEN  L+ 
Sbjct: 25  GGPRIRSPPGKGNKPVALADITN-TGKPNAARSIT------------VPDLVKENTKLLT 84

Query: 70  LIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQHELGC 129
           L+ E+ +II++S  E+ KLR   Q  +QQNL L QTNSQMLAE+N+GKDR+K LQHEL C
Sbjct: 85  LLNEKTKIIDLSRVEIYKLRLALQASKQQNLHLTQTNSQMLAEINTGKDRIKMLQHELSC 144

Query: 130 KNGIL------LSRKLD-----------QEIKEKSA--ALQAGEVGTAECN-------EA 189
              +L      L RK +           Q +K K++  A++A  VG +  +       E+
Sbjct: 145 TTALLKVKDSELDRKKNAGNVQQKGVKSQVLKTKASTVAVEAHHVGDSVTSGVEHHVVES 204

Query: 190 EESMHTN-------KDTRPCKANRPRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFK 249
           + ++ +N       +D +  +  + R+S R + G+  ++   Q    + P V    +   
Sbjct: 205 QSAVSSNTVCQEPPQDGKQKRMPQRRRSSRLNQGSCEIRGVSQNTLHENPVVPVAPSTLS 264

Query: 250 TEEPVAST--KELSEIENFNS-----------------------TDFSQCKETSVVQTEV 309
            E+    T  K +  ++N  S                       TD  +  E    +TEV
Sbjct: 265 LEKQYGQTTGKHMKSLQNECSATVHEVIMASEFEKTEINELPQKTDLKEIPEACSSETEV 324

Query: 310 QKAE-SKRPCNRRQSSRFKIEEPVA--TKDLFEIENSHFIDASQCKETSV---------I 369
           Q  +   +  N +Q+     +  ++  T D  E    + +     K +S+         I
Sbjct: 325 QSHKIGDKAFNSKQNHLTGSQSSLSFNTVDTPEPPEDNTVKRCSKKRSSIEDVNAKLDTI 384

Query: 370 QTEVQKVEDKRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTA--VSS 406
            +E  + E+KR   R+ SAR  +    ++  D+V       + +K+V+  +A +   VS 
Sbjct: 385 TSEPLRHEEKRKSRRKISARLNSVS--SEHTDIV-----VETEHKDVIVSLAGSTSNVSM 444

BLAST of MC04g1459 vs. NCBI nr
Match: XP_022133446.1 (SHUGOSHIN 2 [Momordica charantia])

HSP 1 Score: 726 bits (1873), Expect = 2.04e-261
Identity = 406/459 (88.45%), Postives = 406/459 (88.45%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 60
           V GPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL
Sbjct: 14  VGGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL
Sbjct: 74  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 133

Query: 121 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR
Sbjct: 134 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 193

Query: 181 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
           PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ
Sbjct: 194 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 300
           CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ
Sbjct: 254 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 313

Query: 301 TEVQKVEDKR-------------------------------------------------- 360
           TEVQKVEDKR                                                  
Sbjct: 314 TEVQKVEDKRFCKRRQSARFRTDEPLATKDLFEIENSHSIAASQCEETSVLQTGVQKVEI 373

Query: 361 --PCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD 407
             PCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD
Sbjct: 374 KRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD 433

BLAST of MC04g1459 vs. NCBI nr
Match: XP_038884301.1 (SHUGOSHIN 2 [Benincasa hispida])

HSP 1 Score: 533 bits (1374), Expect = 2.87e-186
Identity = 306/413 (74.09%), Postives = 342/413 (82.81%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 60
           V G NMKI GEK +KSSKVGG QRKRLSDISNLKEQPTLQKRDT  Q SLLMT+E VDKL
Sbjct: 14  VGGQNMKIAGEKTMKSSKVGGGQRKRLSDISNLKEQPTLQKRDTMPQPSLLMTHEYVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMK+IAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQ NSQMLAELNSGK+RL
Sbjct: 74  QKENMTLMKVIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQANSQMLAELNSGKERL 133

Query: 121 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGIL+SRKLD E K KSA L+ GEVGT EC+EAEES++ N+D RPCK NR
Sbjct: 134 KALQHELGCKNGILMSRKLDLERKGKSATLEPGEVGTTECSEAEESINANQDNRPCKTNR 193

Query: 181 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
            RQS+RES GTS LQ EV +VE KRPC+ RQ+A+FKTEEPVA+  ++ E EN NS D SQ
Sbjct: 194 RRQSRRESFGTSFLQKEVPRVEGKRPCLRRQSAKFKTEEPVAA-NDILETENSNSNDASQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 300
           CKETSV QTEVQK E KRPC+RRQS+RFK EEPVA  DLF+IE+S+  DASQCKE SV+Q
Sbjct: 254 CKETSVHQTEVQKVEGKRPCSRRQSARFKAEEPVAANDLFQIEDSNSTDASQCKEISVLQ 313

Query: 301 TEVQKVEDKRPCLRRQSARFKTEEPVAKTEDLV---SSSTSASSLYKEVVCE---IAPTA 360
           T+VQKVE KRPCLRRQS  FK +EPVA  + +    S+STS     +EV+CE   I PT 
Sbjct: 314 TDVQKVEGKRPCLRRQSTGFKLKEPVAVKDSVEIENSNSTSTFLCKEEVMCEVGKIVPT- 373

Query: 361 VSSVGREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 407
            SSV +E  GN+TD+ EVQECRR SVGRP RRAAEKV SYKEIP+NVKMRRQ+
Sbjct: 374 -SSVEKEVYGNSTDRSEVQECRRISVGRPSRRAAEKVISYKEIPLNVKMRRQI 423

BLAST of MC04g1459 vs. NCBI nr
Match: XP_008456540.1 (PREDICTED: shugoshin-1 isoform X1 [Cucumis melo] >TYJ99967.1 shugoshin-1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 530 bits (1366), Expect = 3.67e-185
Identity = 299/409 (73.11%), Postives = 339/409 (82.89%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 60
           VVG N+KI GEK +KSSK    QRKRLSDISNLKEQPTLQKRDTK Q  LLMTYE VDKL
Sbjct: 14  VVGQNVKIAGEKTMKSSK---GQRKRLSDISNLKEQPTLQKRDTKTQPGLLMTYEYVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMK+IAERNRIIEISGNELEKLRTNFQKLQQQN+QLAQ N QMLAELNS KDRL
Sbjct: 74  QKENMTLMKVIAERNRIIEISGNELEKLRTNFQKLQQQNMQLAQANCQMLAELNSSKDRL 133

Query: 121 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGIL+SRKL  E K KSA LQ GEVGT ECNEAEES+  N+D RPCK+NR
Sbjct: 134 KALQHELGCKNGILMSRKLVLESKGKSATLQPGEVGTTECNEAEESISANQDNRPCKSNR 193

Query: 181 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
            RQS+RES GTS LQTEV ++E KRPC+ +Q+A+FKTEEPVA+  ++ E EN NS D SQ
Sbjct: 194 KRQSRRESFGTSSLQTEVPKIEGKRPCLRQQSAKFKTEEPVAA-NDILETENSNSNDASQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 300
           CKETSV+QTEVQK E KRPC+RRQS+RFK EEPV T DL +IE S   +ASQC+ETSV+Q
Sbjct: 254 CKETSVLQTEVQKVEGKRPCSRRQSARFKAEEPVNTNDLQQIETSDSTNASQCQETSVLQ 313

Query: 301 TEVQKVEDKRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLY--KEVVCEIAPTAVSSV 360
            E+QKVE KRPCLRRQS RFK EEPVA  + L   +++++S +  KE +CE+ PT  SSV
Sbjct: 314 AEIQKVEGKRPCLRRQSTRFKLEEPVATKDSLEIENSNSTSAFPCKETMCEVVPT--SSV 373

Query: 361 GREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 407
           G+ED  N+ D+ EVQECRR+SVGRP RRAAEKV SYKEIP+NVKMRRQV
Sbjct: 374 GKEDYDNSIDRSEVQECRRTSVGRPSRRAAEKVISYKEIPVNVKMRRQV 416

BLAST of MC04g1459 vs. NCBI nr
Match: XP_022992974.1 (SHUGOSHIN 2-like isoform X7 [Cucurbita maxima])

HSP 1 Score: 525 bits (1352), Expect = 5.68e-183
Identity = 299/405 (73.83%), Postives = 339/405 (83.70%), Query Frame = 0

Query: 5   NMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKLQKEN 64
           NM+I G K +KSSKVGGSQRKRLSDISNLKEQP LQKRDTK QSSLL TYE VDKLQKEN
Sbjct: 19  NMEIAGVKTMKSSKVGGSQRKRLSDISNLKEQPILQKRDTKPQSSLLKTYEYVDKLQKEN 78

Query: 65  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 124
           M L K++AERNRIIEISGNELE LRTNFQKLQQQNLQ AQ NSQMLAELNSGK+RLKALQ
Sbjct: 79  MALTKVLAERNRIIEISGNELETLRTNFQKLQQQNLQFAQANSQMLAELNSGKERLKALQ 138

Query: 125 HELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANRPRQS 184
           HELGCKNGIL+SRKLD E K KSA  Q G VGTA+ +EA ESM+T +D RPCK NR RQS
Sbjct: 139 HELGCKNGILMSRKLDLERKGKSATFQPGGVGTAKSSEAAESMNTKQDNRPCKTNRRRQS 198

Query: 185 KRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQCKET 244
           ++ES GTSVLQTEVQ+VE KRP   RQ+ARFKTEEPVA+  ++ E +N NS + SQCKET
Sbjct: 199 RQESFGTSVLQTEVQKVEGKRP--RRQSARFKTEEPVAA-NDILETDNSNSNNSSQCKET 258

Query: 245 SVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQTEVQ 304
           SV + EVQK E KRPC+RRQS+R KIEEPVAT DL  IEN +  DASQC+ETSV+QTEVQ
Sbjct: 259 SVRRAEVQKVEGKRPCSRRQSARLKIEEPVATNDLLGIENFNSTDASQCRETSVLQTEVQ 318

Query: 305 KVEDKRPCLRRQSARFKTEEPVAKTE--DLVSSSTSASSLYKEVVCEIAPTAVSSVGRED 364
           KVE  RPCLRRQSARFK EEPVA  +  D V+S+++++SL KE+VCEI     SSV  +D
Sbjct: 319 KVEGIRPCLRRQSARFKLEEPVAIKDSLDTVNSNSTSASLCKEIVCEIKIVPTSSVETKD 378

Query: 365 DGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 407
           +GN+TD+ EVQECRR+SVGRPLRRAAEK+QSYKEIP+NVKMRR V
Sbjct: 379 NGNSTDRSEVQECRRTSVGRPLRRAAEKIQSYKEIPLNVKMRRPV 420

BLAST of MC04g1459 vs. NCBI nr
Match: XP_011649425.1 (SHUGOSHIN 2 isoform X1 [Cucumis sativus] >KGN62194.1 hypothetical protein Csa_006836 [Cucumis sativus])

HSP 1 Score: 524 bits (1350), Expect = 1.14e-182
Identity = 297/410 (72.44%), Postives = 335/410 (81.71%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 60
           VVG N+ + GEK + SSKVGG QRKRLSDISN KEQPTLQKRDTK Q  LLMT E VDKL
Sbjct: 14  VVGQNVNVAGEKTMGSSKVGGGQRKRLSDISNWKEQPTLQKRDTKTQPGLLMTCEYVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMK+IAERNRIIEISG ELEKLRTNFQKLQQQN+QLAQTNSQMLAELNS KDRL
Sbjct: 74  QKENMTLMKVIAERNRIIEISGKELEKLRTNFQKLQQQNMQLAQTNSQMLAELNSCKDRL 133

Query: 121 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGIL+SRKLD E K KSA LQ GEVGT ECNEAEES + N+D RPCK+NR
Sbjct: 134 KALQHELGCKNGILMSRKLDLESKGKSATLQPGEVGTTECNEAEESTNANQDNRPCKSNR 193

Query: 181 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
            RQS+R+S GTS LQTEV ++E KRPC+ +Q+A FKTEEP+A+  ++ E EN NS D SQ
Sbjct: 194 RRQSRRQSFGTSSLQTEVPKIEGKRPCLRQQSAMFKTEEPLAA-NDILETENSNSNDASQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 300
           CKETSV+QTEVQK ESKRPC+RRQS+RFK EEPV T DL ++E S   +  QC+ETSV+Q
Sbjct: 254 CKETSVLQTEVQKVESKRPCSRRQSARFKAEEPVTTNDLHQMETSDSTNTPQCQETSVLQ 313

Query: 301 TEVQKVEDKRPCLRRQSARFKTEEPVAKTEDLV---SSSTSASSLYKEVVCEIAPTAVSS 360
            E+QKVE KRPCLRRQS +FK EEPVA  + L    S+STS +   KE  CE+ PT  SS
Sbjct: 314 AEIQKVEVKRPCLRRQSTKFKLEEPVAIKDSLEIENSNSTSTAFPCKETKCEVVPT--SS 373

Query: 361 VGREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 407
           VG+ED  N+ D  EVQECRR+SVGRP RRAAEKV SYKEIP+NVKMRRQV
Sbjct: 374 VGKEDYDNSIDISEVQECRRTSVGRPSRRAAEKVISYKEIPLNVKMRRQV 420

BLAST of MC04g1459 vs. ExPASy TrEMBL
Match: A0A6J1BV49 (SHUGOSHIN 2 OS=Momordica charantia OX=3673 GN=LOC111006020 PE=3 SV=1)

HSP 1 Score: 726 bits (1873), Expect = 9.88e-262
Identity = 406/459 (88.45%), Postives = 406/459 (88.45%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 60
           V GPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL
Sbjct: 14  VGGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL
Sbjct: 74  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 133

Query: 121 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR
Sbjct: 134 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 193

Query: 181 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
           PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ
Sbjct: 194 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 300
           CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ
Sbjct: 254 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 313

Query: 301 TEVQKVEDKR-------------------------------------------------- 360
           TEVQKVEDKR                                                  
Sbjct: 314 TEVQKVEDKRFCKRRQSARFRTDEPLATKDLFEIENSHSIAASQCEETSVLQTGVQKVEI 373

Query: 361 --PCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD 407
             PCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD
Sbjct: 374 KRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD 433

BLAST of MC04g1459 vs. ExPASy TrEMBL
Match: A0A5D3BLG7 (Shugoshin-1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00080 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 1.78e-185
Identity = 299/409 (73.11%), Postives = 339/409 (82.89%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 60
           VVG N+KI GEK +KSSK    QRKRLSDISNLKEQPTLQKRDTK Q  LLMTYE VDKL
Sbjct: 14  VVGQNVKIAGEKTMKSSK---GQRKRLSDISNLKEQPTLQKRDTKTQPGLLMTYEYVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMK+IAERNRIIEISGNELEKLRTNFQKLQQQN+QLAQ N QMLAELNS KDRL
Sbjct: 74  QKENMTLMKVIAERNRIIEISGNELEKLRTNFQKLQQQNMQLAQANCQMLAELNSSKDRL 133

Query: 121 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGIL+SRKL  E K KSA LQ GEVGT ECNEAEES+  N+D RPCK+NR
Sbjct: 134 KALQHELGCKNGILMSRKLVLESKGKSATLQPGEVGTTECNEAEESISANQDNRPCKSNR 193

Query: 181 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
            RQS+RES GTS LQTEV ++E KRPC+ +Q+A+FKTEEPVA+  ++ E EN NS D SQ
Sbjct: 194 KRQSRRESFGTSSLQTEVPKIEGKRPCLRQQSAKFKTEEPVAA-NDILETENSNSNDASQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 300
           CKETSV+QTEVQK E KRPC+RRQS+RFK EEPV T DL +IE S   +ASQC+ETSV+Q
Sbjct: 254 CKETSVLQTEVQKVEGKRPCSRRQSARFKAEEPVNTNDLQQIETSDSTNASQCQETSVLQ 313

Query: 301 TEVQKVEDKRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLY--KEVVCEIAPTAVSSV 360
            E+QKVE KRPCLRRQS RFK EEPVA  + L   +++++S +  KE +CE+ PT  SSV
Sbjct: 314 AEIQKVEGKRPCLRRQSTRFKLEEPVATKDSLEIENSNSTSAFPCKETMCEVVPT--SSV 373

Query: 361 GREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 407
           G+ED  N+ D+ EVQECRR+SVGRP RRAAEKV SYKEIP+NVKMRRQV
Sbjct: 374 GKEDYDNSIDRSEVQECRRTSVGRPSRRAAEKVISYKEIPVNVKMRRQV 416

BLAST of MC04g1459 vs. ExPASy TrEMBL
Match: A0A1S3C330 (shugoshin-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496463 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 1.78e-185
Identity = 299/409 (73.11%), Postives = 339/409 (82.89%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 60
           VVG N+KI GEK +KSSK    QRKRLSDISNLKEQPTLQKRDTK Q  LLMTYE VDKL
Sbjct: 14  VVGQNVKIAGEKTMKSSK---GQRKRLSDISNLKEQPTLQKRDTKTQPGLLMTYEYVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMK+IAERNRIIEISGNELEKLRTNFQKLQQQN+QLAQ N QMLAELNS KDRL
Sbjct: 74  QKENMTLMKVIAERNRIIEISGNELEKLRTNFQKLQQQNMQLAQANCQMLAELNSSKDRL 133

Query: 121 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGIL+SRKL  E K KSA LQ GEVGT ECNEAEES+  N+D RPCK+NR
Sbjct: 134 KALQHELGCKNGILMSRKLVLESKGKSATLQPGEVGTTECNEAEESISANQDNRPCKSNR 193

Query: 181 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
            RQS+RES GTS LQTEV ++E KRPC+ +Q+A+FKTEEPVA+  ++ E EN NS D SQ
Sbjct: 194 KRQSRRESFGTSSLQTEVPKIEGKRPCLRQQSAKFKTEEPVAA-NDILETENSNSNDASQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 300
           CKETSV+QTEVQK E KRPC+RRQS+RFK EEPV T DL +IE S   +ASQC+ETSV+Q
Sbjct: 254 CKETSVLQTEVQKVEGKRPCSRRQSARFKAEEPVNTNDLQQIETSDSTNASQCQETSVLQ 313

Query: 301 TEVQKVEDKRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLY--KEVVCEIAPTAVSSV 360
            E+QKVE KRPCLRRQS RFK EEPVA  + L   +++++S +  KE +CE+ PT  SSV
Sbjct: 314 AEIQKVEGKRPCLRRQSTRFKLEEPVATKDSLEIENSNSTSAFPCKETMCEVVPT--SSV 373

Query: 361 GREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 407
           G+ED  N+ D+ EVQECRR+SVGRP RRAAEKV SYKEIP+NVKMRRQV
Sbjct: 374 GKEDYDNSIDRSEVQECRRTSVGRPSRRAAEKVISYKEIPVNVKMRRQV 416

BLAST of MC04g1459 vs. ExPASy TrEMBL
Match: A0A6J1JYY1 (SHUGOSHIN 2-like isoform X7 OS=Cucurbita maxima OX=3661 GN=LOC111489140 PE=3 SV=1)

HSP 1 Score: 525 bits (1352), Expect = 2.75e-183
Identity = 299/405 (73.83%), Postives = 339/405 (83.70%), Query Frame = 0

Query: 5   NMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKLQKEN 64
           NM+I G K +KSSKVGGSQRKRLSDISNLKEQP LQKRDTK QSSLL TYE VDKLQKEN
Sbjct: 19  NMEIAGVKTMKSSKVGGSQRKRLSDISNLKEQPILQKRDTKPQSSLLKTYEYVDKLQKEN 78

Query: 65  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 124
           M L K++AERNRIIEISGNELE LRTNFQKLQQQNLQ AQ NSQMLAELNSGK+RLKALQ
Sbjct: 79  MALTKVLAERNRIIEISGNELETLRTNFQKLQQQNLQFAQANSQMLAELNSGKERLKALQ 138

Query: 125 HELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANRPRQS 184
           HELGCKNGIL+SRKLD E K KSA  Q G VGTA+ +EA ESM+T +D RPCK NR RQS
Sbjct: 139 HELGCKNGILMSRKLDLERKGKSATFQPGGVGTAKSSEAAESMNTKQDNRPCKTNRRRQS 198

Query: 185 KRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQCKET 244
           ++ES GTSVLQTEVQ+VE KRP   RQ+ARFKTEEPVA+  ++ E +N NS + SQCKET
Sbjct: 199 RQESFGTSVLQTEVQKVEGKRP--RRQSARFKTEEPVAA-NDILETDNSNSNNSSQCKET 258

Query: 245 SVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQTEVQ 304
           SV + EVQK E KRPC+RRQS+R KIEEPVAT DL  IEN +  DASQC+ETSV+QTEVQ
Sbjct: 259 SVRRAEVQKVEGKRPCSRRQSARLKIEEPVATNDLLGIENFNSTDASQCRETSVLQTEVQ 318

Query: 305 KVEDKRPCLRRQSARFKTEEPVAKTE--DLVSSSTSASSLYKEVVCEIAPTAVSSVGRED 364
           KVE  RPCLRRQSARFK EEPVA  +  D V+S+++++SL KE+VCEI     SSV  +D
Sbjct: 319 KVEGIRPCLRRQSARFKLEEPVAIKDSLDTVNSNSTSASLCKEIVCEIKIVPTSSVETKD 378

Query: 365 DGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 407
           +GN+TD+ EVQECRR+SVGRPLRRAAEK+QSYKEIP+NVKMRR V
Sbjct: 379 NGNSTDRSEVQECRRTSVGRPLRRAAEKIQSYKEIPLNVKMRRPV 420

BLAST of MC04g1459 vs. ExPASy TrEMBL
Match: A0A0A0LMD0 (Shugoshin_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G326470 PE=3 SV=1)

HSP 1 Score: 524 bits (1350), Expect = 5.54e-183
Identity = 297/410 (72.44%), Postives = 335/410 (81.71%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 60
           VVG N+ + GEK + SSKVGG QRKRLSDISN KEQPTLQKRDTK Q  LLMT E VDKL
Sbjct: 14  VVGQNVNVAGEKTMGSSKVGGGQRKRLSDISNWKEQPTLQKRDTKTQPGLLMTCEYVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMK+IAERNRIIEISG ELEKLRTNFQKLQQQN+QLAQTNSQMLAELNS KDRL
Sbjct: 74  QKENMTLMKVIAERNRIIEISGKELEKLRTNFQKLQQQNMQLAQTNSQMLAELNSCKDRL 133

Query: 121 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGIL+SRKLD E K KSA LQ GEVGT ECNEAEES + N+D RPCK+NR
Sbjct: 134 KALQHELGCKNGILMSRKLDLESKGKSATLQPGEVGTTECNEAEESTNANQDNRPCKSNR 193

Query: 181 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
            RQS+R+S GTS LQTEV ++E KRPC+ +Q+A FKTEEP+A+  ++ E EN NS D SQ
Sbjct: 194 RRQSRRQSFGTSSLQTEVPKIEGKRPCLRQQSAMFKTEEPLAA-NDILETENSNSNDASQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 300
           CKETSV+QTEVQK ESKRPC+RRQS+RFK EEPV T DL ++E S   +  QC+ETSV+Q
Sbjct: 254 CKETSVLQTEVQKVESKRPCSRRQSARFKAEEPVTTNDLHQMETSDSTNTPQCQETSVLQ 313

Query: 301 TEVQKVEDKRPCLRRQSARFKTEEPVAKTEDLV---SSSTSASSLYKEVVCEIAPTAVSS 360
            E+QKVE KRPCLRRQS +FK EEPVA  + L    S+STS +   KE  CE+ PT  SS
Sbjct: 314 AEIQKVEVKRPCLRRQSTKFKLEEPVAIKDSLEIENSNSTSTAFPCKETKCEVVPT--SS 373

Query: 361 VGREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 407
           VG+ED  N+ D  EVQECRR+SVGRP RRAAEKV SYKEIP+NVKMRRQV
Sbjct: 374 VGKEDYDNSIDISEVQECRRTSVGRPSRRAAEKVISYKEIPLNVKMRRQV 420

BLAST of MC04g1459 vs. TAIR 10
Match: AT5G04320.2 (Shugoshin C terminus )

HSP 1 Score: 146.0 bits (367), Expect = 6.7e-35
Identity = 156/499 (31.26%), Postives = 233/499 (46.69%), Query Frame = 0

Query: 6   MKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLM-TYECVDKLQKEN 65
           M +T   +  S++V GSQ+       N+K+   + K +T+Q+ ++L  + E   KLQKEN
Sbjct: 1   MVLTEMSLDYSNRVLGSQK------DNVKQ---MDKEETQQKENMLFSSQEYAAKLQKEN 60

Query: 66  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 125
           MTLMK +A RN+++E+SG E++KLR N + +Q++NLQLAQ NSQMLAELN+ +DRLK LQ
Sbjct: 61  MTLMKALAHRNKLVELSGIEIQKLRINLRSVQEKNLQLAQANSQMLAELNTNRDRLKDLQ 120

Query: 126 HELGCKNGILLSRK-LDQEI----KEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKAN 185
           HELGCKN +L  +K L++++      +S    +      +C   +     +KDT+  +  
Sbjct: 121 HELGCKNALLKVKKHLEEQVLPRTHHESKDKVSASASDGDCKSFQVHDIKHKDTKRKRTT 180

Query: 186 RPRQS-------------------KRESSG---TSVLQTEVQQVEDKRPCVTRQTARFKT 245
           R + S                   KR  SG   T+ +  E+ Q ED              
Sbjct: 181 RIKSSVSADVKPIPVNDSNSKANRKRRVSGVIDTTGIPEEICQTED------------DI 240

Query: 246 EEPVASTKELSEIENFNSTDFSQCKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATK 305
           ++ V S     +I+N  +  F       V ++       KR C RRQS+RF ++E   T+
Sbjct: 241 DKGVVSRGVNQDIDNVVNKKFVPDAANPVKES----VHRKRQCTRRQSTRFDVQETKQTE 300

Query: 306 DLFEIENSHFIDASQCKETSVIQ----------------------TEVQKVEDKRPCLRR 365
            L E++      A + KET+                          EV++   +R    R
Sbjct: 301 KLLEMDG-----AKESKETASFSLRRRSARLRHEEAEPCKSLHEGDEVRETIKRRRVSLR 360

Query: 366 QSARFKTEEP----VAKTEDLVS------------------------------------S 407
            SARF  +EP     +  +D  S                                     
Sbjct: 361 LSARFDIQEPHVTETSNADDARSIVIEESAGSRSESVEPSESRHETKEITRKRSFSTRRQ 420

BLAST of MC04g1459 vs. TAIR 10
Match: AT5G04320.1 (Shugoshin C terminus )

HSP 1 Score: 129.0 bits (323), Expect = 8.5e-30
Identity = 136/439 (30.98%), Postives = 199/439 (45.33%), Query Frame = 0

Query: 65  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 124
           MTLMK +A RN+++E+SG E++KLR N + +Q++NLQLAQ NSQMLAELN+ +DRLK LQ
Sbjct: 1   MTLMKALAHRNKLVELSGIEIQKLRINLRSVQEKNLQLAQANSQMLAELNTNRDRLKDLQ 60

Query: 125 HELGCKNGILLSRK-LDQEI----KEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKAN 184
           HELGCKN +L  +K L++++      +S    +      +C   +     +KDT+  +  
Sbjct: 61  HELGCKNALLKVKKHLEEQVLPRTHHESKDKVSASASDGDCKSFQVHDIKHKDTKRKRTT 120

Query: 185 RPRQS-------------------KRESSG---TSVLQTEVQQVEDKRPCVTRQTARFKT 244
           R + S                   KR  SG   T+ +  E+ Q ED              
Sbjct: 121 RIKSSVSADVKPIPVNDSNSKANRKRRVSGVIDTTGIPEEICQTED------------DI 180

Query: 245 EEPVASTKELSEIENFNSTDFSQCKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATK 304
           ++ V S     +I+N  +  F       V ++       KR C RRQS+RF ++E   T+
Sbjct: 181 DKGVVSRGVNQDIDNVVNKKFVPDAANPVKES----VHRKRQCTRRQSTRFDVQETKQTE 240

Query: 305 DLFEIENSHFIDASQCKETSVIQ----------------------TEVQKVEDKRPCLRR 364
            L E++      A + KET+                          EV++   +R    R
Sbjct: 241 KLLEMDG-----AKESKETASFSLRRRSARLRHEEAEPCKSLHEGDEVRETIKRRRVSLR 300

Query: 365 QSARFKTEEP----VAKTEDLVS------------------------------------S 407
            SARF  +EP     +  +D  S                                     
Sbjct: 301 LSARFDIQEPHVTETSNADDARSIVIEESAGSRSESVEPSESRHETKEITRKRSFSTRRQ 360

BLAST of MC04g1459 vs. TAIR 10
Match: AT3G10440.1 (Shugoshin C terminus )

HSP 1 Score: 115.2 bits (287), Expect = 1.3e-25
Identity = 127/423 (30.02%), Postives = 206/423 (48.70%), Query Frame = 0

Query: 7   KITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTK--QQSSLLMTYECVDKLQKEN 66
           K  GEKM+    +  +QR++L DI+NL+ Q  L  +  K  QQ+ L+ + E  + LQKEN
Sbjct: 21  KAKGEKMVLEPPMNSAQRRKLGDITNLQNQKNLMNQGAKHQQQAILISSKENAENLQKEN 80

Query: 67  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 126
             LMK++ ER+ I     ++L+KLR  FQK+Q+QNL LAQ N+++LAE N+ KD+LK LQ
Sbjct: 81  TKLMKVVMERDGI----KSDLKKLRIEFQKVQEQNLLLAQANTRILAEFNTSKDQLKVLQ 140

Query: 127 HELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAEC--NEAEESMHTNKDTRPCKANRPR 186
           HELGCKNG++++RK+   +KE+          T  C  + ++     N     CK  +P 
Sbjct: 141 HELGCKNGLVMARKM--LLKEQ----------TLPCTRHASKVKAQANACGGACKTFQPN 200

Query: 187 QSKRE----SSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIE-NFNSTD 246
            +  E    SS  + LQ   +    +R    +  A  +  + +  + E  ++E N ++  
Sbjct: 201 DADHEHASGSSNANSLQRNEKANSKRRVSGRKNPANSEVLDIIGRSGETCQMEDNIDNKK 260

Query: 247 FSQCKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFI--DA--SQC 306
                +           +SKR C  RQSS  K  E   T+ L ++ ++  I  DA  S  
Sbjct: 261 LVSDSDNDAENHINDNVQSKRYCAGRQSSSSKTREASQTETLQKVVDAKEIKGDARFSLT 320

Query: 307 KETSVIQTE----VQKVEDKRPCLRRQSARFKTEEPVAKT--EDLVSSSTSASSLYKEVV 366
           K +  ++++     + + + R  LRR+SAR K++EP       D + ++    S  +  +
Sbjct: 321 KHSDWLKSQEPEPSESLYESRFPLRRRSARLKSQEPEPSESFHDSIETTKRRRSAIRSAM 380

Query: 367 CEIAPTAV--SSVGREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEI--PINVKM 407
             I    V  +  G  DD     K       +S+  +P    A +    KEI     + +
Sbjct: 381 FNIQELGVIQNLNGLPDDQEIAAKARCSAREQSTGSKP---EAVEPHDTKEIIGKSRISL 424