MC04g1423 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g1423
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	vesicle-associated membrane protein 727
Location	MC04: 22184100 .. 22188030 (+)
RNA-Seq Expression	MC04g1423
Synteny	MC04g1423

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGTATTTAAACACATCTCAGAGACTTGTTCCTGGAAGGAGACGGAAGGTTAACTGATTGAGGTTTGGGTTCCCTATAAATTACCCGTCTGTTTGCCAATTTTATGTGTTCTTCAAACTGATCGATCTTTTAGCTTGCATCGTGCGTGAAGTTGACTAGAGATTTCAAGTTTAAGGTTCGCAATTTCTCCGTTGTCCTCTGTTTCGTACTTTCTGATTGGATCGTGGAATTTAATTGTGTTTTCTTCGTCGATTTCTGGATGATTCTGTTTAAGGGTTTGATTTGTTTCGCGAACTGTTCCTTAAGAACTTCTAATTCGGGTTCAGAGCGAGAATCGTTGCATGAGTTGTAAGAAACGCTGTGAATTTGCATTTGTTTGATCTTCTTCGTTTTCGTTTTCTTCTTTGCGTAAGCTTGAAGTCGTTTTGTGCAATCGGGCTGATTTGAAATTTGTGGTTTCGTTGGGATTCGGAGTTTACTTCTCTACACCTTTGTCAAGGGTTCATTAATTGCCTGAAGGTACTTGGGGTTTATCGTCTACAGTTTGATGTTTGATGCAATTACGTATTGGGTGTTGCCATTTGTATAAATTAGTTATTGTGTTGGATTCGAACCTTACTGCTAGTGATAAAAAAAAAAAAAAGCTCCTAGTTCTTCGATAGACCAATCTTACAAGCAAGAAATTTACTTACCGTTCCCTCCTTTATTAGTTTTGGCTCAATTTTTATGGACCGGTAGTACGATAACTTCCCGAAAAATAGGAATTAACTTAGCTGGACATCATTCGACTAAGACCTAGGAATTTTGAATAACAAACATAAAAGTCCCAATGAGCCCAGTAACAAATTGGATGAGAATTGAATAAAGGCAATAAAAAGGGTAGCCTCATATTGTGTTGTTAACTAAGATATTAGTGAGTTTTCCTATGTTTCCTTCCTCCGGGTTGAGAAAAAATTCAATTGACACTATCCATTTAAAATGTTTGTAGGTTACTCCTGCAGTTCGGTGTGCTACTAATTTGATTCTTCACGGAAAACAGTGGGAGATGAGTCAGAAAGGCCTGATATATAGCTTTGTTGCTAAAGGAACTGTTGTTTTAGCGGAACACACATCATTCTCAGGGAATTTTAGTACCATAGCCGTTCAATGCTTGCAGAAATTGCCTTCTAATAGCAGCAAATGCACATACTCATGTGATGGGCACACATTCAACTTCCTTCTTGATAGTGGATTTGGTATTGTGTTTTCTCAACTTTGATAATATACTTTCTTAGACTTCATCAACACTTGTGGCTGAAGGTGTTTGATGGATTTGCAGTTTTTCTGGCTGTTGCGGATGAATCAGTTGGAAGGAATGTGCCATTTGTATTTCTTGACCGTGTGAAAGATGACTTTAAGCAGCGTTATGGTTCAAGTATCAAGGATGAGAACCCTCACCCACTTGCAGATGATGAAGATGACGATGATTTATTTCTGGATCGATTCAGTGTTGCATACACTCTTGACCGAGAATTTGGGTATGCCCATGGCCATGTTTATGAACAATGTTAGAGTTCTATGTCATACATTTGACCTAACAACTTCTAATTTAATTTTCATGATAGGCCAAGGCTTAAGGAGCACATGCAATATTGCATGAGCCATCCAGAAGAAATGAGTAAACTTTCCAAGCTGAAGGCTCAAATAACAGAAGTGAAAGGCATTATGATGGACAACATCGAAAAGGTTAGACCTCTTGTTTTATAAAATCACACAATTCCTGCTAAAGATTTTGTATCGAGATTTTGTCTGTATATAATATATATGTTTTTCATCTATTTGTTTTTTTTCACAGTTGTGCATAATGAAAAGATACGGAGACAGAGATTAAAGCATTTTAGAAAATCTCACTTTATGCACTCTAATTCTTCCTCCTTTTCCATGTCATCTTGGCAAATTGTATAAAATCACATTAACATTGTGTCTGGGCATATTCTTGTCACATGTGGCTTCATTGTTGGTAGACAGCCAAGTAATAATATGCTAGCCATGAGTACTGAACAAATTATTTTATCTCTTTAAGTACCGCAGTTAGGAGTAGTAATTATGTTGCGACTTGCGAGGAAATGAATAAAAAAAAATTACATTAGTGTGTTGCTTGCTTGTTTATGAAGAACCAGACTTTCTGCTGGACCAGTTGAATAATTGTTTGAAAATAATTTGATAGTAGTCTAGTTATTTTGGAAAGAAGCGGAATTTCATTAGTCAAATATTTTATTTGTAAATTAAACACCATCATATGTTTTTCTCACACACATGCACGTACATATATATGAAGTCTTTGGCTAGGCCCAATGGTCTGTGACTCCATGTACAAAATCAGTTGTGAAAACCTGAATATTACATAAGGCACAAGCCCTAGAGGTCTCTTTTTTGCTTCATCTTGTTTATTTTGTGTTCATAACCAATAAGTAACTCAAAGTAATGGAAGAATGAAATTGGAATGCATTGTGATGATTTTGCGCAATGAAGCATAACTGGCCTTGTCGAAGACACCATAAAGTGTCAATTTGATAGGATTTTACATTTTATTGTTATTGCTTTCCTACTTTGAGCTTGCAAGTTTTTGAATACATACAAAAAATCCTGTGTGTGCTGACCTGCCGAGTAAGTTACATGTATGTAATAGAGAATCCTTTTCAGACCTTGGCGGCTAAGGGATGCTAGACCTTTTTAAGACGAGACTATTGACAATTCACTTTTTATATTTCTTTAAGGAGCTCTCTTGGTAATAGGTTATTTTGTTACTAGCCTCTCACTATGATTGGAATTTCTTAAAATCCTTGTGGTTTTGGGTTGGGTAATGCCTCATCAGTCCTTGCATACTATTCCACATTTTAAATGGTCTGGAATTGGTGAATCAGTACTTTGGTCTTCCACATTCTCATAATATTAAACTTCAGTTGTCATTTTTTTTAAATTTTTGAGAAAAGTAAAGGCAATTGTTGAGTGACACCATTAAAGTCACCACCGAATTGAGATCTTGCTTGGGTTTAGCAAGAAAAAAAGTAGACCATCCTTTTTGTACACTTTGAGTAATAACTACTCTATGTATTTGTATTTTTTTCCTATAAATAAGAAGAGAGAGAGAGAGATGCTAAGAAATTCCTTTTGGAAAGAAACTGAAAATTAGAAGAATTTTCTTCCAGTTAGTTCTGCCCTAGCCGCTAAGTTCTGAGTACCAAGGACAGGCAAATTGGGGGCTTCGGAACTCTATAACTTATCTTTCTGAGCTATTCTAAACAGATATGATAGCAATTGCCTGTCCCTCGTGTAGGTTCTAGACCGCGGAGAGCGGATTGAGCTTCTGGTAGACAAGACAGAAAATCTACAATTTCAGGTACCTCATTTAATCTGCCTGTCTGTTGAGAACTCTGAATGGGAAGTGATCTATCTAATCTGTTTATCGTTGGGATCTCTGTTTGAACTTAGGCCGATAACTTTCATCGACAAGGGAGGCAACTTCGGCGAAAAATGTGGCTGCAGAGCTTACAGATGAAGCTGATGGTTGGGGGAGGAATCTTGGTCATGATCATCATAATTTGGTTTATTGCATGCGGAGGTTTCAAATGTTGAATTTGGTAAAGTTAGTAGCAGATGAGTGTTTTTTCACTTGGGTAATGGCTATGTTTGGAAACTGGCACCAAATCTATATAATACGCCTCTTGTTCTAACTTGTAATTAGCATTCTATATTACCTACCAGTTAAAATTTATTTGTTGGGTTTCTTCTGTCTCTTGTGTATCGAAGAATCTGTGTTAAAAGTGAAATAACCTTACACTCTTTAGATAATAGTGAAATAACCTTACACTCTTTAGATAATGCTCAATTTGTTTCCACTGTTACTAATGAAAAAATCGTCCAAATCGTATTAGAATGGATGAGTCGTGGA

mRNA sequence

GAGTATTTAAACACATCTCAGAGACTTGTTCCTGGAAGGAGACGGAAGGTTAACTGATTGAGGTTTGGGTTCCCTATAAATTACCCGTCTGTTTGCCAATTTTATGTGTTCTTCAAACTGATCGATCTTTTAGCTTGCATCGTGCGTGAAGTTGACTAGAGATTTCAAGTTTAAGGTTACTCCTGCAGTTCGGTGTGCTACTAATTTGATTCTTCACGGAAAACAGTGGGAGATGAGTCAGAAAGGCCTGATATATAGCTTTGTTGCTAAAGGAACTGTTGTTTTAGCGGAACACACATCATTCTCAGGGAATTTTAGTACCATAGCCGTTCAATGCTTGCAGAAATTGCCTTCTAATAGCAGCAAATGCACATACTCATGTGATGGGCACACATTCAACTTCCTTCTTGATAGTGGATTTGTTTTTCTGGCTGTTGCGGATGAATCAGTTGGAAGGAATGTGCCATTTGTATTTCTTGACCGTGTGAAAGATGACTTTAAGCAGCGTTATGGTTCAAGTATCAAGGATGAGAACCCTCACCCACTTGCAGATGATGAAGATGACGATGATTTATTTCTGGATCGATTCAGTGTTGCATACACTCTTGACCGAGAATTTGGGCCAAGGCTTAAGGAGCACATGCAATATTGCATGAGCCATCCAGAAGAAATGAGTAAACTTTCCAAGCTGAAGGCTCAAATAACAGAAGTGAAAGGCATTATGATGGACAACATCGAAAAGGTTCTAGACCGCGGAGAGCGGATTGAGCTTCTGGTAGACAAGACAGAAAATCTACAATTTCAGGCCGATAACTTTCATCGACAAGGGAGGCAACTTCGGCGAAAAATGTGGCTGCAGAGCTTACAGATGAAGCTGATGGTTGGGGGAGGAATCTTGGTCATGATCATCATAATTTGGTTTATTGCATGCGGAGGTTTCAAATGTTGAATTTGGTAAAGTTAGTAGCAGATGAGTGTTTTTTCACTTGGGTAATGGCTATGTTTGGAAACTGGCACCAAATCTATATAATACGCCTCTTGTTCTAACTTGTAATTAGCATTCTATATTACCTACCAGTTAAAATTTATTTGTTGGGTTTCTTCTGTCTCTTGTGTATCGAAGAATCTGTGTTAAAAGTGAAATAACCTTACACTCTTTAGATAATAGTGAAATAACCTTACACTCTTTAGATAATGCTCAATTTGTTTCCACTGTTACTAATGAAAAAATCGTCCAAATCGTATTAGAATGGATGAGTCGTGGA

Coding sequence (CDS)

ATGAGTCAGAAAGGCCTGATATATAGCTTTGTTGCTAAAGGAACTGTTGTTTTAGCGGAACACACATCATTCTCAGGGAATTTTAGTACCATAGCCGTTCAATGCTTGCAGAAATTGCCTTCTAATAGCAGCAAATGCACATACTCATGTGATGGGCACACATTCAACTTCCTTCTTGATAGTGGATTTGTTTTTCTGGCTGTTGCGGATGAATCAGTTGGAAGGAATGTGCCATTTGTATTTCTTGACCGTGTGAAAGATGACTTTAAGCAGCGTTATGGTTCAAGTATCAAGGATGAGAACCCTCACCCACTTGCAGATGATGAAGATGACGATGATTTATTTCTGGATCGATTCAGTGTTGCATACACTCTTGACCGAGAATTTGGGCCAAGGCTTAAGGAGCACATGCAATATTGCATGAGCCATCCAGAAGAAATGAGTAAACTTTCCAAGCTGAAGGCTCAAATAACAGAAGTGAAAGGCATTATGATGGACAACATCGAAAAGGTTCTAGACCGCGGAGAGCGGATTGAGCTTCTGGTAGACAAGACAGAAAATCTACAATTTCAGGCCGATAACTTTCATCGACAAGGGAGGCAACTTCGGCGAAAAATGTGGCTGCAGAGCTTACAGATGAAGCTGATGGTTGGGGGAGGAATCTTGGTCATGATCATCATAATTTGGTTTATTGCATGCGGAGGTTTCAAATGTTGA

Protein sequence

MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLDSGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFSVAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIELLVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC

Homology

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BLAST of MC04g1423 vs. ExPASy Swiss-Prot
Match: Q9M376 (Vesicle-associated membrane protein 727 OS=Arabidopsis thaliana OX=3702 GN=VAMP727 PE=1 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.0e-112
Identity = 201/238 (84.45%), Postives = 221/238 (92.86%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHT +SGNFSTIAVQCLQKLP+NSSK TYSCDGHTFNFL+D
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GFVFL VADES GR+VPFVFL+RVK+DFK+RY +SIK++  HPLAD+++DDDLF DRFS
Sbjct: 61  NGFVFLVVADESTGRSVPFVFLERVKEDFKKRYEASIKNDERHPLADEDEDDDLFGDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAY LDREFGP LKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGE+IEL
Sbjct: 121 VAYNLDREFGPILKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 239
           LVDKTENLQFQAD+F RQGRQLRRKMWLQSLQMKLMV G +   I+I+W +ACGGFKC
Sbjct: 181 LVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGAVFSFILIVWVVACGGFKC 238

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BLAST of MC04g1423 vs. ExPASy Swiss-Prot
Match: O48850 (Vesicle-associated membrane protein 725 OS=Arabidopsis thaliana OX=3702 GN=VAMP725 PE=2 SV=2)

HSP 1 Score: 276.6 bits (706), Expect = 2.7e-73
Identity = 135/238 (56.72%), Postives = 180/238 (75.63%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+L E+T F GNF+ +A QCLQKLPS+++K TY+CDGHTFN+L++
Sbjct: 66  MGQQNLIYSFVARGTVILVEYTEFKGNFTAVAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 125

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA ESVGR +P  FL+RVK+DF +RYG                         +
Sbjct: 126 NGFTYCVVAVESVGRQIPMAFLERVKEDFNKRYGGGKATT-------------------A 185

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L+REFG +LKEHMQYC+ HP+E+SKL+K+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 186 QANSLNREFGSKLKEHMQYCVDHPDEISKLAKVKAQVTEVKGVMMENIEKVLDRGEKIEL 245

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 239
           LVDKTENL+ QA +F  QG ++RRKMW +++++KL+V G I+ +I+II    CGGFKC
Sbjct: 246 LVDKTENLRSQAQDFRTQGTKIRRKMWFENMKIKLIVLGIIITLILIIILSVCGGFKC 284

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BLAST of MC04g1423 vs. ExPASy Swiss-Prot
Match: Q9MAS5 (Putative vesicle-associated membrane protein 726 OS=Arabidopsis thaliana OX=3702 GN=VAMP726 PE=2 SV=2)

HSP 1 Score: 271.6 bits (693), Expect = 8.7e-72
Identity = 133/238 (55.88%), Postives = 177/238 (74.37%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+LAE+T F GNF+++A QCLQKLPS+++K TY+CDGHTFN+L D
Sbjct: 1   MGQQSLIYSFVARGTVILAEYTEFKGNFTSVAAQCLQKLPSSNNKFTYNCDGHTFNYLAD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  V  ES GR +P  FL+RVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVVIESAGRQIPMAFLERVKEDFNKRYGGGKAST-------------------A 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYC  HPEE+SKLSK+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 KANSLNKEFGSKLKEHMQYCADHPEEISKLSKVKAQVTEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 239
           LVDKTENL+ QA +F  QG +++RK+W +++++KL+V G I+ +I+II    C GFKC
Sbjct: 181 LVDKTENLRSQAQDFRTQGTKMKRKLWFENMKIKLIVFGIIVALILIIILSVCHGFKC 219

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BLAST of MC04g1423 vs. ExPASy Swiss-Prot
Match: P47192 (Vesicle-associated membrane protein 722 OS=Arabidopsis thaliana OX=3702 GN=VAMP722 PE=2 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 1.5e-71
Identity = 133/238 (55.88%), Postives = 178/238 (74.79%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           M+Q+ LIYSFVA+GTV+L E T F GNF++IA QCLQKLPS+++K TY+CDGHTFN+L++
Sbjct: 1   MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA +S GR +P  FL+RVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVAVDSAGRQIPMAFLERVKEDFNKRYGGG-------------------KAATA 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYCM HP+E+SKL+K+KAQ++EVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 QANSLNKEFGSKLKEHMQYCMDHPDEISKLAKVKAQVSEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 239
           LVDKTENL+ QA +F  QG Q+RRKMW Q++++KL+V   I+ +I+II    CGGF C
Sbjct: 181 LVDKTENLRSQAQDFRTQGTQMRRKMWFQNMKIKLIVLAIIIALILIIILSICGGFNC 219

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BLAST of MC04g1423 vs. ExPASy Swiss-Prot
Match: Q9ZTW3 (Vesicle-associated membrane protein 721 OS=Arabidopsis thaliana OX=3702 GN=VAMP721 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 9.6e-71
Identity = 133/238 (55.88%), Postives = 177/238 (74.37%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           M+Q+ LIYSFVA+GTV+L E T F GNF++IA QCLQKLPS+++K TY+CDGHTFN+L++
Sbjct: 1   MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
            GF +  VA +S GR +P  FL+RVK+DF +RYG                         +
Sbjct: 61  DGFTYCVVAVDSAGRQIPMSFLERVKEDFNKRYGGG-------------------KAATA 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYCM HP+E+SKL+K+KAQ++EVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 QANSLNKEFGSKLKEHMQYCMDHPDEISKLAKVKAQVSEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 239
           LVDKTENL+ QA +F   G Q+RRKMWLQ++++KL+V   I+ +I+II    C GFKC
Sbjct: 181 LVDKTENLRSQAQDFRTTGTQMRRKMWLQNMKIKLIVLAIIIALILIIVLSVCHGFKC 219

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BLAST of MC04g1423 vs. NCBI nr
Match: XP_022134273.1 (vesicle-associated membrane protein 727 [Momordica charantia])

HSP 1 Score: 473 bits (1218), Expect = 2.57e-168
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238

[Close]

BLAST of MC04g1423 vs. NCBI nr
Match: XP_038884037.1 (vesicle-associated membrane protein 727 [Benincasa hispida])

HSP 1 Score: 464 bits (1195), Expect = 8.24e-165
Identity = 230/238 (96.64%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQ+LPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRN+PFVFL+RVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCM+HPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGIL++III+WFI CGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 238

[Close]

BLAST of MC04g1423 vs. NCBI nr
Match: XP_008456603.1 (PREDICTED: vesicle-associated membrane protein 727 [Cucumis melo] >TYK11767.1 vesicle-associated membrane protein 727 [Cucumis melo var. makuwa])

HSP 1 Score: 464 bits (1195), Expect = 8.24e-165
Identity = 231/238 (97.06%), Postives = 236/238 (99.16%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQ+LPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRN+PFVFL+RVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILV+ II+WFI CGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFIILWFIVCGGFKC 238

[Close]

BLAST of MC04g1423 vs. NCBI nr
Match: XP_004140905.1 (vesicle-associated membrane protein 727 [Cucumis sativus] >KGN46041.1 hypothetical protein Csa_005451 [Cucumis sativus])

HSP 1 Score: 463 bits (1191), Expect = 3.36e-164
Identity = 229/238 (96.22%), Postives = 236/238 (99.16%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKG+VVLAEHTSFSGNFSTIAVQCLQ+LPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGSVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRN+PFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGP+LKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPKLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILV+ +I+WFI CGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFVILWFIVCGGFKC 238

[Close]

BLAST of MC04g1423 vs. NCBI nr
Match: XP_022939856.1 (vesicle-associated membrane protein 727-like [Cucurbita moschata] >XP_022939857.1 vesicle-associated membrane protein 727-like [Cucurbita moschata] >KAG7016346.1 Vesicle-associated membrane protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 462 bits (1190), Expect = 4.77e-164
Identity = 229/238 (96.22%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRN+PFVFL+RVKDDFKQRYGSSIKDENPHPLADDED+DDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDEDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPR+KEHMQYCM+HPEEM+KLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRIKEHMQYCMNHPEEMNKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILV+III+WFI CGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 238

[Close]

BLAST of MC04g1423 vs. ExPASy TrEMBL
Match: A0A6J1C1J9 (vesicle-associated membrane protein 727 OS=Momordica charantia OX=3673 GN=LOC111006575 PE=3 SV=1)

HSP 1 Score: 473 bits (1218), Expect = 1.24e-168
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238

[Close]

BLAST of MC04g1423 vs. ExPASy TrEMBL
Match: A0A5D3CIL2 (Vesicle-associated membrane protein 727 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold304G00880 PE=3 SV=1)

HSP 1 Score: 464 bits (1195), Expect = 3.99e-165
Identity = 231/238 (97.06%), Postives = 236/238 (99.16%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQ+LPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRN+PFVFL+RVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILV+ II+WFI CGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFIILWFIVCGGFKC 238

[Close]

BLAST of MC04g1423 vs. ExPASy TrEMBL
Match: A0A1S3C3Q2 (vesicle-associated membrane protein 727 OS=Cucumis melo OX=3656 GN=LOC103496513 PE=3 SV=1)

HSP 1 Score: 464 bits (1195), Expect = 3.99e-165
Identity = 231/238 (97.06%), Postives = 236/238 (99.16%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQ+LPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRN+PFVFL+RVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILV+ II+WFI CGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFIILWFIVCGGFKC 238

[Close]

BLAST of MC04g1423 vs. ExPASy TrEMBL
Match: A0A0A0KE69 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G045200 PE=3 SV=1)

HSP 1 Score: 463 bits (1191), Expect = 1.62e-164
Identity = 229/238 (96.22%), Postives = 236/238 (99.16%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKG+VVLAEHTSFSGNFSTIAVQCLQ+LPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGSVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRN+PFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGP+LKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPKLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILV+ +I+WFI CGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFVILWFIVCGGFKC 238

[Close]

BLAST of MC04g1423 vs. ExPASy TrEMBL
Match: A0A6J1FGZ7 (vesicle-associated membrane protein 727-like OS=Cucurbita moschata OX=3662 GN=LOC111445601 PE=3 SV=1)

HSP 1 Score: 462 bits (1190), Expect = 2.31e-164
Identity = 229/238 (96.22%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRN+PFVFL+RVKDDFKQRYGSSIKDENPHPLADDED+DDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDEDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPR+KEHMQYCM+HPEEM+KLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRIKEHMQYCMNHPEEMNKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 238
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILV+III+WFI CGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 238

[Close]

BLAST of MC04g1423 vs. TAIR 10
Match: AT3G54300.1 (vesicle-associated membrane protein 727 )

HSP 1 Score: 407.5 bits (1046), Expect = 7.2e-114
Identity = 201/238 (84.45%), Postives = 221/238 (92.86%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHT +SGNFSTIAVQCLQKLP+NSSK TYSCDGHTFNFL+D
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GFVFL VADES GR+VPFVFL+RVK+DFK+RY +SIK++  HPLAD+++DDDLF DRFS
Sbjct: 61  NGFVFLVVADESTGRSVPFVFLERVKEDFKKRYEASIKNDERHPLADEDEDDDLFGDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAY LDREFGP LKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGE+IEL
Sbjct: 121 VAYNLDREFGPILKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 239
           LVDKTENLQFQAD+F RQGRQLRRKMWLQSLQMKLMV G +   I+I+W +ACGGFKC
Sbjct: 181 LVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGAVFSFILIVWVVACGGFKC 238

[Close]

BLAST of MC04g1423 vs. TAIR 10
Match: AT3G54300.2 (vesicle-associated membrane protein 727 )

HSP 1 Score: 407.5 bits (1046), Expect = 7.2e-114
Identity = 201/238 (84.45%), Postives = 221/238 (92.86%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHT +SGNFSTIAVQCLQKLP+NSSK TYSCDGHTFNFL+D
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GFVFL VADES GR+VPFVFL+RVK+DFK+RY +SIK++  HPLAD+++DDDLF DRFS
Sbjct: 61  NGFVFLVVADESTGRSVPFVFLERVKEDFKKRYEASIKNDERHPLADEDEDDDLFGDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAY LDREFGP LKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGE+IEL
Sbjct: 121 VAYNLDREFGPILKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 239
           LVDKTENLQFQAD+F RQGRQLRRKMWLQSLQMKLMV G +   I+I+W +ACGGFKC
Sbjct: 181 LVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGAVFSFILIVWVVACGGFKC 238

[Close]

BLAST of MC04g1423 vs. TAIR 10
Match: AT2G32670.1 (vesicle-associated membrane protein 725 )

HSP 1 Score: 276.6 bits (706), Expect = 1.9e-74
Identity = 135/238 (56.72%), Postives = 180/238 (75.63%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+L E+T F GNF+ +A QCLQKLPS+++K TY+CDGHTFN+L++
Sbjct: 66  MGQQNLIYSFVARGTVILVEYTEFKGNFTAVAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 125

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA ESVGR +P  FL+RVK+DF +RYG                         +
Sbjct: 126 NGFTYCVVAVESVGRQIPMAFLERVKEDFNKRYGGGKATT-------------------A 185

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L+REFG +LKEHMQYC+ HP+E+SKL+K+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 186 QANSLNREFGSKLKEHMQYCVDHPDEISKLAKVKAQVTEVKGVMMENIEKVLDRGEKIEL 245

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 239
           LVDKTENL+ QA +F  QG ++RRKMW +++++KL+V G I+ +I+II    CGGFKC
Sbjct: 246 LVDKTENLRSQAQDFRTQGTKIRRKMWFENMKIKLIVLGIIITLILIIILSVCGGFKC 284

[Close]

BLAST of MC04g1423 vs. TAIR 10
Match: AT1G04760.1 (vesicle-associated membrane protein 726 )

HSP 1 Score: 271.6 bits (693), Expect = 6.2e-73
Identity = 133/238 (55.88%), Postives = 177/238 (74.37%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+LAE+T F GNF+++A QCLQKLPS+++K TY+CDGHTFN+L D
Sbjct: 1   MGQQSLIYSFVARGTVILAEYTEFKGNFTSVAAQCLQKLPSSNNKFTYNCDGHTFNYLAD 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  V  ES GR +P  FL+RVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVVIESAGRQIPMAFLERVKEDFNKRYGGGKAST-------------------A 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYC  HPEE+SKLSK+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 KANSLNKEFGSKLKEHMQYCADHPEEISKLSKVKAQVTEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 239
           LVDKTENL+ QA +F  QG +++RK+W +++++KL+V G I+ +I+II    C GFKC
Sbjct: 181 LVDKTENLRSQAQDFRTQGTKMKRKLWFENMKIKLIVFGIIVALILIIILSVCHGFKC 219

[Close]

BLAST of MC04g1423 vs. TAIR 10
Match: AT2G33120.1 (synaptobrevin-related protein 1 )

HSP 1 Score: 270.8 bits (691), Expect = 1.1e-72
Identity = 133/238 (55.88%), Postives = 178/238 (74.79%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60
           M+Q+ LIYSFVA+GTV+L E T F GNF++IA QCLQKLPS+++K TY+CDGHTFN+L++
Sbjct: 1   MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 60

Query: 61  SGFVFLAVADESVGRNVPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA +S GR +P  FL+RVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVAVDSAGRQIPMAFLERVKEDFNKRYGGG-------------------KAATA 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYCM HP+E+SKL+K+KAQ++EVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 QANSLNKEFGSKLKEHMQYCMDHPDEISKLAKVKAQVSEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVMIIIIWFIACGGFKC 239
           LVDKTENL+ QA +F  QG Q+RRKMW Q++++KL+V   I+ +I+II    CGGF C
Sbjct: 181 LVDKTENLRSQAQDFRTQGTQMRRKMWFQNMKIKLIVLAIIIALILIIILSICGGFNC 219

The following BLAST results are available for this feature:

BLAST of MC04g1423 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M376	1.0e-112	84.45	Vesicle-associated membrane protein 727 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]
O48850	2.7e-73	56.72	Vesicle-associated membrane protein 725 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]
Q9MAS5	8.7e-72	55.88	Putative vesicle-associated membrane protein 726 OS=Arabidopsis thaliana OX=3702...	[more]
P47192	1.5e-71	55.88	Vesicle-associated membrane protein 722 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]
Q9ZTW3	9.6e-71	55.88	Vesicle-associated membrane protein 721 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]

BLAST of MC04g1423 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134273.1	2.57e-168	100.00	vesicle-associated membrane protein 727 [Momordica charantia]	[more]
XP_038884037.1	8.24e-165	96.64	vesicle-associated membrane protein 727 [Benincasa hispida]	[more]
XP_008456603.1	8.24e-165	97.06	PREDICTED: vesicle-associated membrane protein 727 [Cucumis melo] >TYK11767.1 ve...	[more]
XP_004140905.1	3.36e-164	96.22	vesicle-associated membrane protein 727 [Cucumis sativus] >KGN46041.1 hypothetic...	[more]
XP_022939856.1	4.77e-164	96.22	vesicle-associated membrane protein 727-like [Cucurbita moschata] >XP_022939857....	[more]

BLAST of MC04g1423 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1J9	1.24e-168	100.00	vesicle-associated membrane protein 727 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5D3CIL2	3.99e-165	97.06	Vesicle-associated membrane protein 727 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3C3Q2	3.99e-165	97.06	vesicle-associated membrane protein 727 OS=Cucumis melo OX=3656 GN=LOC103496513 ...	[more]
A0A0A0KE69	1.62e-164	96.22	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G045200 PE=3 SV=1	[more]
A0A6J1FGZ7	2.31e-164	96.22	vesicle-associated membrane protein 727-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of MC04g1423 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G54300.1	7.2e-114	84.45	vesicle-associated membrane protein 727	[more]
AT3G54300.2	7.2e-114	84.45	vesicle-associated membrane protein 727	[more]
AT2G32670.1	1.9e-74	56.72	vesicle-associated membrane protein 725	[more]
AT1G04760.1	6.2e-73	55.88	vesicle-associated membrane protein 726	[more]
AT2G33120.1	1.1e-72	55.88	synaptobrevin-related protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001388	Synaptobrevin	PRINTS	PR00219	SYNAPTOBREVN	coord: 154..173 score: 50.41 coord: 210..229 score: 26.35 coord: 174..193 score: 42.43
IPR001388	Synaptobrevin	PFAM	PF00957	Synaptobrevin	coord: 148..234 e-value: 3.8E-32 score: 109.9
IPR001388	Synaptobrevin	PROSITE	PS00417	SYNAPTOBREVIN	coord: 167..186
IPR010908	Longin domain	SMART	SM01270	Longin_2	coord: 31..111 e-value: 9.3E-22 score: 88.3
IPR010908	Longin domain	PFAM	PF13774	Longin	coord: 33..100 e-value: 3.6E-18 score: 65.4
IPR010908	Longin domain	PROSITE	PS50859	LONGIN	coord: 6..133 score: 23.700758
IPR010908	Longin domain	CDD	cd14824	Longin	coord: 7..140 e-value: 1.93861E-32 score: 112.734
None	No IPR available	GENE3D	3.30.450.50	Longin domain	coord: 1..111 e-value: 5.8E-39 score: 135.6
None	No IPR available	GENE3D	1.20.5.110		coord: 123..233 e-value: 1.6E-34 score: 120.1
None	No IPR available	PANTHER	PTHR21136:SF169	VESICLE-ASSOCIATED MEMBRANE PROTEIN 727	coord: 1..238
None	No IPR available	PANTHER	PTHR21136	SNARE PROTEINS	coord: 1..238
None	No IPR available	CDD	cd15843	R-SNARE	coord: 149..207 e-value: 1.51509E-29 score: 103.351
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 144..209
IPR042855	v-SNARE, coiled-coil homology domain	PROSITE	PS50892	V_SNARE	coord: 149..209 score: 19.343924
IPR011012	Longin-like domain superfamily	SUPERFAMILY	64356	SNARE-like	coord: 6..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g1423.1	MC04g1423.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016192	vesicle-mediated transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle