MC04g1387 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g1387
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionGFO_IDH_MocA domain-containing protein
LocationMC04: 21849845 .. 21853597 (+)
RNA-Seq ExpressionMC04g1387
SyntenyMC04g1387
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTACACTATCATTTTTATCTAAATCTTATCAAATTCATACCAATTATAATAAATTTTCAATTGGAAACTTGCCAAAGAGTATAAATTACAAACCTTAATCGCATTTGTCACCCTCGAGGATGAAAAAAAAAACTTGCTCTTTAAAGTTAAGAAAGAAATGTAAAACTAAAAAAATTATAAAAAAATAATGAGTTCTGACATATTTAGCGGTGTTGCTCAACTTCGAGGAGCCATATTAAAAAAAAAAAAAAAAAAAACATTTGACCTTTACCTCACTTTTCCCCATTTTTGGAGCATATAAATTTCATGAATTATTTCACATCAAAGTTCAAACACGTCCCCATCAGTCATCACCTGCTCCCTTATCTCCATCACTGTTTCCAGCAACGTCCAAAGCTTCGAGCGAGCGCGGATATGGCCGACGCCACCGTCCGTTTCGGAATCATCGGCTGCGCCGAGATAGCTCGGAAGGTTGCCAGAGCCATTGCCCTTGCTCCCAACGCCACGCTCTCGGCAATCGGTAGTCGTACACTCGAGAAGGCCTCGAAATTTGCGTCCGATAATGGCTTCACTCCTCGAGTCAAAATCTATGGCTCTTATGAAGCTGTCCTCGATGATCCCGAAGTCGACGCCGTCTATGTCCCCCTTCCGACTAGTCTTCACCTGCGCTGGGCCGTGCTTGCTGCAGAGAAGAAGAAGCACTTGCTCCTCGAGAAGCCTGTGGCTCTTAATGTAGCGGAGTTGGACAAGATACTCGAAGCGTGTGAGTCCAATGGAGTTCAGTTCATGGACGGTACCATGTGGATGCACAACCCTCGCACGGCCAAGATGAAGGAGTTTCTTTCCGATGCAGACCAGTTTGGGCAGCTCAAATCGGTATGCGAGTCAATTTCCATTTACAACGTTGTAATTCATCTGGTTGAATCTATGACTGAATGTGCTCGTGCGGGAAATTCGAAATATCCTAAATGCAATTTGAATAATTTGGTTTCCTACTCGAGGAGTTTTAATATTTTATGCTAAGCATCAATTTTGTGGTAATTTCTGAACGCCTCTGTTATTTCTTGTATAATGGATATGATACACTGCAGATCGGCCTGTTAGGAGCGCCACTAGTCTGAAATTTTTGTTTAGAAAAATTGGAGTCAAAATATGGGTTGTTAGCTTAATCAAGTTGAGAGATAGAAACTTCTCTAAAAATGACTTTGTTTAGCATAATAATTTCAATCACGATAACAGATGGCAGCGGGCAAAAGAATGAGTTGAGGTATGATTTCATATTTTGTGGAAAAAGAAGAGGAAGAACATGTGATTACTTTGTGAAATTCTGTATGGTCATTTAGAGCATTTGGAAGGAAACTAAACAAAAGGATTTGATAAGTTTGATGATGGGGTTGATTTATTACATTTATTTACTTATAATTATGTATTATTGGACGTTTTGATGTGATATTTACCTGGTTGTTCTGTTTTTGCAAGTGAGATTTCTTGTCTGCGTTATTCCTTGTATCTAGATATCTATCTTTGATGATAGTTTGTTACATTTTTCGAAAAATCATATCAGTTTCAAAAAGTATTGTATTGTCATTACAAAATAGAAATACGCGGAAGCCAGTAGTGGACCGTCTGCTATCGGGACAGCCAGCCCAATCTTATAATTGAATTGTTTTTATTCTTGTTCTTTGGGGTTTTCCTTGCTGATTATTGAACTCACTGGATTTCCCTGAAGGTTAAAACCATTTCATCTTATGTGAGTTCAGTTCCTTTGGTCCTTTTGACAGGAATGGTTAACCACTTATCAAGTATAGGTAGTCTAGGAGGAAGAATGGAGGAAATTTGCATGACTAGTTAGTTAGTTCATATCGCGATAGGGCGGATATCTTTTGTACAGTAGAGGATGTGGGACCCATTCGAGCGAGGTAGTTAGAGTAAGCACAAAATAGATGATGCAAACAGTAGGGAGAGTCAAGAAATGCTTGTGGATAACTAAAAGTGTTATTTTGAAATGCTTGTGGATAACTAAAAGTGTTATTTTGAAATGCTTGTGGATAACTAAAAGTGTTATTTTGAAATGCTTGTGGATAACTAAAAGTGTTATTTTGAAATGCTTGTGGATAGCTAAAAGTGTTATCCACTTTGAAATGCTTTTTTATGACATGCCTTTTGAAATGCTTGTGGATAACTAAAAGTGTTATTTAAAGATTTGTAGCGATGTTTTTGAAGAGTGTTTTTAACCAATTAAAAGTACTTTACAAAATTTTTTATTATTCAAAGTATTTTAAAAAATTATAAAATTAGATAAAAAGCACTTAAAAGTGCTTTTGTACAGAACATTTAATTAGTTTTCTTTTATAAGTGTTTGCAGTTTCACTTAAGATATCCAATATTCACCCTTACTTTCACAGGAATAAAGATTGTAAATAAGGATTTTTTTTTAGTGCAGCCTTTCCTACTTGGAAAATAGGTGTGCAGGAGCAGGAGTATATGGTCTGTCTTGTTTGAACAAGATGAAATGAAATGGGTTGCTCTTTACTTTTTATCGTCACAATCAGATTGGGCTTTGCTATTCCAAATTTGATGATAACTTCATATTTGTTATTATATATCCTAACATTGCAATTATATGCCGTGGTAGTGCTGAGTGCTGACATATGGTAAAGTGTTTATAAGGTTTCAGTGAATTAGTTTTAACTTTTAGGCTAAAAGTTTGTTAATTTAATTGTTGAGGAGAAGATTTAACTCTTGTGTTATTTACTACTTTTTGCACATTGTTTTCGTCGTACTTAAGCCAGTCAGTTATTAAAAGTTGAAATTTTCTTCTGGATTTGGATAATTCGTTGACAAATGGTTTGTCTATTTTATGTTCTGAATATCTTAGGTACACAGCATTTTTTCTTTTACTGCTGATGCTGACTTTTTGGCTAATGATATTCGTGTTAAATCCGACCTTGATGGCCTTGGTGCTTTGGGAGATGCCGGGTGGTATTGTATTCGTTCAATCCTTTTTGCTGCTGACTTCGAGCTGCCTAAAAGAGTGATTGCCTTGCCTAATCCCGTCTTGAATGAATCGGGGGTGATTTTGTCGTGTGGAGCTTCTCTCTATTGGGATGATGGGAAAGTAGCAACCTTCCATTGCTCTTTCTTGTCAAACTTGACCATGGATATGACTGCCATAGGAACAAATGGGGCATTACATGTTAATGATTTTATCATACCTTACGGAGAGAAGGAGGCCCATTTCACTACTGGTTCTAAATCTGGCTTCACCGAACTCGTGACTGGTTGGGAACCATTACCAAGCAAACACATCGTCCCAACCGATCTTCCTCAGGAGGCTCGCATGGTGAGAGAGTTTTCCCATTTGGTTAAAGAGATCAAAGCAAATGGCTCCAAACCTGAGAAAAAGTGGCCAACCATCTCTCGGAAGACTCAGCTCGTGGTCGATGCAGTGAAGGCCTCACTCGATCGAGGATCCGAACCCGTCGAAGTAGGGAAGTGATTCCACTGACCTTTTCATATCTTGTTGCTGTATGATGCTTTGTATTGAACTTCAAATTGTTGGTCTATGGGTGTGAAGTGGTTGGACCAAGCTTAGCTTCTATTGATATGTATGATGTTTAGGCTTGCATCAAGCTTGGGAAGCTCTGCATTCATCAAAGATTTACTGAAGTCTTGAAGAGCTAAAGACAATGAGAGAAGCCCTTCCTTGAAAATAATTGCATGCTTTTGGTCAATTTAGTTTGTGTTGCTCT

mRNA sequence

GCTACACTATCATTTTTATCTAAATCTTATCAAATTCATACCAATTATAATAAATTTTCAATTGGAAACTTGCCAAAGAGTATAAATTACAAACCTTAATCGCATTTGTCACCCTCGAGGATGAAAAAAAAAACTTGCTCTTTAAAGTTAAGAAAGAAATGTAAAACTAAAAAAATTATAAAAAAATAATGAGTTCTGACATATTTAGCGGTGTTGCTCAACTTCGAGGAGCCATATTAAAAAAAAAAAAAAAAAAAACATTTGACCTTTACCTCACTTTTCCCCATTTTTGGAGCATATAAATTTCATGAATTATTTCACATCAAAGTTCAAACACGTCCCCATCAGTCATCACCTGCTCCCTTATCTCCATCACTGTTTCCAGCAACGTCCAAAGCTTCGAGCGAGCGCGGATATGGCCGACGCCACCGTCCGTTTCGGAATCATCGGCTGCGCCGAGATAGCTCGGAAGGTTGCCAGAGCCATTGCCCTTGCTCCCAACGCCACGCTCTCGGCAATCGGTAGTCGTACACTCGAGAAGGCCTCGAAATTTGCGTCCGATAATGGCTTCACTCCTCGAGTCAAAATCTATGGCTCTTATGAAGCTGTCCTCGATGATCCCGAAGTCGACGCCGTCTATGTCCCCCTTCCGACTAGTCTTCACCTGCGCTGGGCCGTGCTTGCTGCAGAGAAGAAGAAGCACTTGCTCCTCGAGAAGCCTGTGGCTCTTAATGTAGCGGAGTTGGACAAGATACTCGAAGCGTGTGAGTCCAATGGAGTTCAGTTCATGGACGGTACCATGTGGATGCACAACCCTCGCACGGCCAAGATGAAGGAGTTTCTTTCCGATGCAGACCAGTTTGGGCAGCTCAAATCGGTACACAGCATTTTTTCTTTTACTGCTGATGCTGACTTTTTGGCTAATGATATTCGTGTTAAATCCGACCTTGATGGCCTTGGTGCTTTGGGAGATGCCGGGTGGTATTGTATTCGTTCAATCCTTTTTGCTGCTGACTTCGAGCTGCCTAAAAGAGTGATTGCCTTGCCTAATCCCGTCTTGAATGAATCGGGGGTGATTTTGTCGTGTGGAGCTTCTCTCTATTGGGATGATGGGAAAGTAGCAACCTTCCATTGCTCTTTCTTGTCAAACTTGACCATGGATATGACTGCCATAGGAACAAATGGGGCATTACATGTTAATGATTTTATCATACCTTACGGAGAGAAGGAGGCCCATTTCACTACTGGTTCTAAATCTGGCTTCACCGAACTCGTGACTGGTTGGGAACCATTACCAAGCAAACACATCGTCCCAACCGATCTTCCTCAGGAGGCTCGCATGGTGAGAGAGTTTTCCCATTTGGTTAAAGAGATCAAAGCAAATGGCTCCAAACCTGAGAAAAAGTGGCCAACCATCTCTCGGAAGACTCAGCTCGTGGTCGATGCAGTGAAGGCCTCACTCGATCGAGGATCCGAACCCGTCGAAGTAGGGAAGTGATTCCACTGACCTTTTCATATCTTGTTGCTGTATGATGCTTTGTATTGAACTTCAAATTGTTGGTCTATGGGTGTGAAGTGGTTGGACCAAGCTTAGCTTCTATTGATATGTATGATGTTTAGGCTTGCATCAAGCTTGGGAAGCTCTGCATTCATCAAAGATTTACTGAAGTCTTGAAGAGCTAAAGACAATGAGAGAAGCCCTTCCTTGAAAATAATTGCATGCTTTTGGTCAATTTAGTTTGTGTTGCTCT

Coding sequence (CDS)

ATGAATTATTTCACATCAAAGTTCAAACACGTCCCCATCAGTCATCACCTGCTCCCTTATCTCCATCACTGTTTCCAGCAACGTCCAAAGCTTCGAGCGAGCGCGGATATGGCCGACGCCACCGTCCGTTTCGGAATCATCGGCTGCGCCGAGATAGCTCGGAAGGTTGCCAGAGCCATTGCCCTTGCTCCCAACGCCACGCTCTCGGCAATCGGTAGTCGTACACTCGAGAAGGCCTCGAAATTTGCGTCCGATAATGGCTTCACTCCTCGAGTCAAAATCTATGGCTCTTATGAAGCTGTCCTCGATGATCCCGAAGTCGACGCCGTCTATGTCCCCCTTCCGACTAGTCTTCACCTGCGCTGGGCCGTGCTTGCTGCAGAGAAGAAGAAGCACTTGCTCCTCGAGAAGCCTGTGGCTCTTAATGTAGCGGAGTTGGACAAGATACTCGAAGCGTGTGAGTCCAATGGAGTTCAGTTCATGGACGGTACCATGTGGATGCACAACCCTCGCACGGCCAAGATGAAGGAGTTTCTTTCCGATGCAGACCAGTTTGGGCAGCTCAAATCGGTACACAGCATTTTTTCTTTTACTGCTGATGCTGACTTTTTGGCTAATGATATTCGTGTTAAATCCGACCTTGATGGCCTTGGTGCTTTGGGAGATGCCGGGTGGTATTGTATTCGTTCAATCCTTTTTGCTGCTGACTTCGAGCTGCCTAAAAGAGTGATTGCCTTGCCTAATCCCGTCTTGAATGAATCGGGGGTGATTTTGTCGTGTGGAGCTTCTCTCTATTGGGATGATGGGAAAGTAGCAACCTTCCATTGCTCTTTCTTGTCAAACTTGACCATGGATATGACTGCCATAGGAACAAATGGGGCATTACATGTTAATGATTTTATCATACCTTACGGAGAGAAGGAGGCCCATTTCACTACTGGTTCTAAATCTGGCTTCACCGAACTCGTGACTGGTTGGGAACCATTACCAAGCAAACACATCGTCCCAACCGATCTTCCTCAGGAGGCTCGCATGGTGAGAGAGTTTTCCCATTTGGTTAAAGAGATCAAAGCAAATGGCTCCAAACCTGAGAAAAAGTGGCCAACCATCTCTCGGAAGACTCAGCTCGTGGTCGATGCAGTGAAGGCCTCACTCGATCGAGGATCCGAACCCGTCGAAGTAGGGAAGTGA

Protein sequence

MNYFTSKFKHVPISHHLLPYLHHCFQQRPKLRASADMADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYGSYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNGVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDGLGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHCSFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVPTDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVGK
Homology
BLAST of MC04g1387 vs. ExPASy Swiss-Prot
Match: Q9SZ83 (Uncharacterized oxidoreductase At4g09670 OS=Arabidopsis thaliana OX=3702 GN=At4g09670 PE=1 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 3.1e-138
Identity = 235/356 (66.01%), Postives = 289/356 (81.18%), Query Frame = 0

Query: 39  DATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYGSY 98
           +  +R G++GCA+IARKV+RAI LAPNAT+S + SR+LEKA  FA+ N +    KI+GSY
Sbjct: 4   ETQIRIGVMGCADIARKVSRAIHLAPNATISGVASRSLEKAKAFATANNYPESTKIHGSY 63

Query: 99  EAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNGV 158
           E++L+DPE+DA+YVPLPTSLH+ WA+ AAEK KH+LLEKPVA+NV E DKI++ACE+NGV
Sbjct: 64  ESLLEDPEIDALYVPLPTSLHVEWAIKAAEKGKHILLEKPVAMNVTEFDKIVDACEANGV 123

Query: 159 QFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDGLG 218
           Q MDGTMW+HNPRTA +KEFLSD+++FGQLK+V S FSF  D DFL NDIRVK  LDGLG
Sbjct: 124 QIMDGTMWVHNPRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKNDIRVKPGLDGLG 183

Query: 219 ALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHCSF 278
           ALGDAGWY IR+ L A +FELPK V A P  VLNE+GVILSCGASL W+DG+ AT +CSF
Sbjct: 184 ALGDAGWYAIRATLLANNFELPKTVTAFPGAVLNEAGVILSCGASLSWEDGRTATIYCSF 243

Query: 279 LSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVPTD 338
           L+NLTM++TAIGT G L V+DFIIPY E EA FTT +K+ F +LVT W   PS+H V T+
Sbjct: 244 LANLTMEITAIGTKGTLRVHDFIIPYKETEASFTTSTKAWFNDLVTAWVSPPSEHTVKTE 303

Query: 339 LPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEV 395
           LPQEA MVREF+ LV EIK NG+KP+  WP+ISRKTQLVVDAVK S+D+  + + +
Sbjct: 304 LPQEACMVREFARLVGEIKNNGAKPDGYWPSISRKTQLVVDAVKESVDKNYQQISL 359

BLAST of MC04g1387 vs. ExPASy Swiss-Prot
Match: P49305 (Uncharacterized oxidoreductase ORF334 OS=Rhizobium meliloti OX=382 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.6e-28
Identity = 106/355 (29.86%), Postives = 178/355 (50.14%), Query Frame = 0

Query: 42  VRFGIIGCAEIA-RKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYGSYEA 101
           VRFGIIG A IA  KV  ++  A    + AI SR L++A   A+  G     + YGSY+ 
Sbjct: 4   VRFGIIGTAAIAVEKVIPSMLSAEGLEVVAIASRDLDRARAAATRFGIG---RSYGSYDE 63

Query: 102 VLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNGVQF 161
           +L DPE++AVY+PLP  LH+ WA+ AAE  KH+L EKP+AL+V EL ++++  +  G + 
Sbjct: 64  ILADPEIEAVYIPLPNHLHVHWAIRAAEAGKHVLCEKPLALDVEELSRLIDCRDRTGRRI 123

Query: 162 MDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFS-FTADADFLANDIRVKSDLDGLGA 221
            +  M   +P+  ++ + ++ + + G+++++  +F+    D   + ND  +     G GA
Sbjct: 124 QEAVMIRAHPQWDEIFDIVA-SGEIGEVRAIQGVFTEVNLDPKSIVNDASI-----GGGA 183

Query: 222 LGDAGWYCIRS--ILFAADFELPKRVIALP--NPVLNESGVILSCGASLYWDDGKVATFH 281
           L D G Y I +  ++FAA+   P+RV A+   +PV    G+     A L +  G+ AT  
Sbjct: 184 LYDLGVYPIAAARMVFAAE---PERVFAVSDLDPVF---GIDRLTSAVLLFPGGRQATLV 243

Query: 282 CSFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIV 341
            S    L  ++   GT  ++ + +   P  +        + S             ++ + 
Sbjct: 244 VSTQLALRHNVEIFGTRKSISLKNPFNPTPDDHCRIVLDNGSKLAAAAA-----ETRRVA 303

Query: 342 PTDLPQEARMVREFSHLVKEIKANGSKP-EKKWPTISRKTQLVVDAVKASLDRGS 390
           P D  Q       FS     I++    P E +W   S  T  V++A++ S +RG+
Sbjct: 304 PAD--QYRLQAERFS---AAIRSGSPLPIELEW---SLGTMKVLNAIQRSAERGN 330

BLAST of MC04g1387 vs. ExPASy Swiss-Prot
Match: D4GP30 (D-xylose 1-dehydrogenase (NADP(+)) 2 OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=HVO_B0029 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 6.0e-25
Identity = 89/256 (34.77%), Postives = 120/256 (46.88%), Query Frame = 0

Query: 44  FGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYGSYEAVLD 103
           FGI+G A I  K       A     +AI SR   +AS  A + G       YGSYEA+L 
Sbjct: 3   FGILGTAGIGVKSVIPAVQASEHEAAAIASRDEARASAVADELGIP---TAYGSYEALLA 62

Query: 104 DPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNGVQFMDG 163
           D  +DAVY+PLP  LH  W   AA++  H+L EKP+  +  E   + + CE  GV  M+ 
Sbjct: 63  DDSLDAVYIPLPNGLHADWVRAAADRGLHVLCEKPLTASADETAAVFDYCEDAGVTLMEA 122

Query: 164 TMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDGLGALGDA 223
            M+  +P T +  E +  A + G + SV S FSF       A+DIR+  DL G G++ D 
Sbjct: 123 FMYRFHPLTERAAELV--ASELGAVVSVTSNFSFRLPDG--ADDIRIDPDLAG-GSVMDV 182

Query: 224 GWYCIRSI-LFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHCSFLSNL 283
           G Y + +  LF      P RV A       + GV       L +D G  A    SF +  
Sbjct: 183 GCYAVSAARLFLG---TPDRVYATTTDT-RDCGVDTRMSGVLEYDSGATARVESSFDTPE 242

Query: 284 TMDMTAIGTNGALHVN 299
           T       T+G L  N
Sbjct: 243 TQYYRVQTTDGRLEAN 246

BLAST of MC04g1387 vs. ExPASy Swiss-Prot
Match: Q9ZA33 (dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Streptomyces violaceoruber OX=1935 GN=gra-orf26 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 4.7e-22
Identity = 75/229 (32.75%), Postives = 121/229 (52.84%), Query Frame = 0

Query: 42  VRFGIIGCAEIA-RKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYGSYEA 101
           +R G+ GCA+IA R++  A A +P+   +A+ SR+ EKA   A   G          Y+A
Sbjct: 12  LRIGVAGCADIALRRMLPAFAASPHTEPTAVASRSSEKARAAAETFG----CAAVEGYDA 71

Query: 102 VLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNGVQF 161
           +L+  +VDAVY+PLP +LH  W   A    KH+L EKP+    A+  ++L+     G+  
Sbjct: 72  LLERRDVDAVYIPLPVALHAPWTERALRAGKHVLAEKPLTARAADTARLLDLARERGLVL 131

Query: 162 MDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDGLGAL 221
            +  +++H+     +++ L DA   G ++++ + F+    +   A+DIR ++DLDG GAL
Sbjct: 132 AENYLFVHHSAYTAVRD-LVDAGAIGDVRALSASFTIPPRS---ADDIRYRADLDG-GAL 191

Query: 222 GDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDG 270
            D G Y +R        EL  R   L +  +   GV L  G++L  D G
Sbjct: 192 LDIGVYPLRLASLLLGSELRVRGAVLRHDTVR--GVDLG-GSALLGDPG 228

BLAST of MC04g1387 vs. ExPASy Swiss-Prot
Match: B3TMR8 (dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Actinomadura kijaniata OX=46161 GN=KijD10 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.2e-22
Identity = 79/265 (29.81%), Postives = 125/265 (47.17%), Query Frame = 0

Query: 42  VRFGIIGCAEIA-RKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYGSYEA 101
           +R G+IGCA+IA R+   A+   P   ++AI SR  ++A +F    G  P       Y A
Sbjct: 10  IRVGVIGCADIAWRRALPALEAEPLTEVTAIASRRWDRAKRFTERFGGEP----VEGYPA 69

Query: 102 VLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNGVQF 161
           +L+  +VDAVYVPLP  LH  W   A    KH+L EKP+  +  + +++       G+  
Sbjct: 70  LLERDDVDAVYVPLPAVLHAEWIDRALRAGKHVLAEKPLTTDRPQAERLFAVARERGLLL 129

Query: 162 MDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDGLGAL 221
           M+  M++H+P+  ++ + L D    G+++S  + F+          DIR ++D+ G GAL
Sbjct: 130 MENFMFLHHPQHRQVADML-DEGVIGEIRSFAASFTIPPKPQ---GDIRYQADVGG-GAL 189

Query: 222 GDAGWYCIRS--ILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHCSF 281
            D G Y IR+  +   AD E    V+        +  V++   A L    G  A      
Sbjct: 190 LDIGVYPIRAAGLFLGADLEFVGAVLRHE----RDRDVVVGGNALLTTRQGVTAQLTFGM 249

Query: 282 LSNLTMDMTAIGTNGALHVNDFIIP 304
               T +    G+ G L +N    P
Sbjct: 250 EHAYTNNYEFRGSTGRLWMNRVFTP 261

BLAST of MC04g1387 vs. NCBI nr
Match: XP_022134449.1 (uncharacterized oxidoreductase At4g09670-like [Momordica charantia])

HSP 1 Score: 720 bits (1859), Expect = 2.71e-261
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0

Query: 37  MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 96
           MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG
Sbjct: 1   MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 60

Query: 97  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 156
           SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN
Sbjct: 61  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 120

Query: 157 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 216
           GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG
Sbjct: 121 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 180

Query: 217 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 276
           LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC
Sbjct: 181 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 240

Query: 277 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 336
           SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP
Sbjct: 241 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 300

Query: 337 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVGK 396
           TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVGK
Sbjct: 301 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVGK 360

BLAST of MC04g1387 vs. NCBI nr
Match: XP_023540091.1 (uncharacterized oxidoreductase At4g09670 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 661 bits (1705), Expect = 7.93e-238
Identity = 326/359 (90.81%), Postives = 343/359 (95.54%), Query Frame = 0

Query: 37  MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 96
           MAD  VRFGIIG AEIARKV+RAIALAPNATLSAIGSR++EKASKFA DNGF+P+VKIYG
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 97  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 156
           SYEAVLDDPE+DAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNV+E DKILEACE+N
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 157 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 216
           GVQFMDGTMWMHNPRTA MKEFLSDADQFGQLKSV+S+FSF ADADFLANDIRVKSDLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDADQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 217 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 276
           LGALGDAGWYCIRSILFAA+FELPKRVIA PNPVLNESGVILSCGASL+WDDGK+ATFHC
Sbjct: 181 LGALGDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 277 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 336
           SFLSNLTMD+TAIGTNG LHVNDFIIPYGEKEA+F T SKSGFTELVTGWEPLPSKHIVP
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 337 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVG 395
           TDLPQEA MVREFSHLVKEIKANGSKPEKKWP ISRKTQLV+DAVK SLD G +PVEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKVSLDGGFKPVEVG 359

BLAST of MC04g1387 vs. NCBI nr
Match: XP_022963640.1 (uncharacterized oxidoreductase At4g09670 [Cucurbita moschata] >XP_022963647.1 uncharacterized oxidoreductase At4g09670 [Cucurbita moschata] >KAG7032898.1 putative oxidoreductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 661 bits (1705), Expect = 7.93e-238
Identity = 326/359 (90.81%), Postives = 344/359 (95.82%), Query Frame = 0

Query: 37  MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 96
           MAD  VRFGIIG AEIARKV+RAIALAPNATLSAIGSR++EKASKFA DNGF+P+VKIYG
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 97  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 156
           SYEAVLDDPE+DAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNV+E DKILEACE+N
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 157 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 216
           GVQFMDGTMWMHNPRTA MKEFLSDA+QFGQLKSV+S+FSF ADADFLANDIRVKSDLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 217 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 276
           LGALGDAGWYCIRSILFAA+FELPKRVIA PNPVLNESGVILSCGASL+WDDGK+ATFHC
Sbjct: 181 LGALGDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 277 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 336
           SFLSNLTMD+TAIGTNG LHVNDFIIPYGEKEA+F T SKSGFTELVTGWEPLPSKHIVP
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 337 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVG 395
           TDLPQEA MVREFSHLVKEIKANGSKPEKKWP ISRKTQLV+DAVKASLD G +PVEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of MC04g1387 vs. NCBI nr
Match: XP_022990391.1 (uncharacterized oxidoreductase At4g09670 [Cucurbita maxima] >XP_022990392.1 uncharacterized oxidoreductase At4g09670 [Cucurbita maxima])

HSP 1 Score: 660 bits (1704), Expect = 1.13e-237
Identity = 326/359 (90.81%), Postives = 343/359 (95.54%), Query Frame = 0

Query: 37  MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 96
           MAD  VRFGIIG AEIARKV+RAIALAPNATLSAIGSR++EKASKFA DNGF+P+VKIYG
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 97  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 156
           SYEAVLDDPE+DAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNV+E DKILEACE+N
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 157 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 216
           GVQFMDGTMWMHNPRTA MKEFLSDA+QFGQLKSV+S+FSF ADADFLANDIRVKSDLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 217 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 276
           LGALGDAGWYCIRSILFAA FELPKRVIA PNPVLNESGVILSCGASL+WDDGK+ATFHC
Sbjct: 181 LGALGDAGWYCIRSILFAASFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 277 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 336
           SFLSNLTMD+TAIGTNG LHVNDFIIPYGEKEA+F T SKSGFTELVTGWEPLPSKHIVP
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 337 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVG 395
           TDLPQEA MVREFSHLVKEIKANGSKPEKKWP ISRKTQLV+DAVKASLD G +PVEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of MC04g1387 vs. NCBI nr
Match: KAG6602218.1 (putative oxidoreductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 659 bits (1699), Expect = 6.51e-237
Identity = 324/359 (90.25%), Postives = 343/359 (95.54%), Query Frame = 0

Query: 37  MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 96
           MAD  VRFGIIG AEIARKV+RAIA+APNATLSAIGSR++EKASKFA DNGF+P+VKIYG
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIAIAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 97  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 156
           SYEAVLDDPE+DAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNV+E DKILEACE+N
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 157 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 216
           GVQFMDGTMWMHNPRTA MKEFLSDA+QFGQLKSV+S+FSF ADADFLANDIRVKSDLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 217 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 276
           LGALGDAGWYCIRSILFAA+FELPKRVIA PNPVLNESGVILSCGASL+WDDGK+ATFHC
Sbjct: 181 LGALGDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 277 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 336
           SFLSNLTMD+TAIGTNG LHVNDFIIPYGEKEA+F T SKSGFTELVTGWEPLPSKHIVP
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 337 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVG 395
           TDLPQEA MVREFSHLVKEIKANGSKPEKKWP ISRKTQLV+DAVK SLD G +PVEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKVSLDGGFKPVEVG 359

BLAST of MC04g1387 vs. ExPASy TrEMBL
Match: A0A6J1BYS0 (uncharacterized oxidoreductase At4g09670-like OS=Momordica charantia OX=3673 GN=LOC111006693 PE=4 SV=1)

HSP 1 Score: 720 bits (1859), Expect = 1.31e-261
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0

Query: 37  MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 96
           MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG
Sbjct: 1   MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 60

Query: 97  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 156
           SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN
Sbjct: 61  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 120

Query: 157 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 216
           GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG
Sbjct: 121 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 180

Query: 217 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 276
           LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC
Sbjct: 181 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 240

Query: 277 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 336
           SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP
Sbjct: 241 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 300

Query: 337 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVGK 396
           TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVGK
Sbjct: 301 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVGK 360

BLAST of MC04g1387 vs. ExPASy TrEMBL
Match: A0A6J1HFT3 (uncharacterized oxidoreductase At4g09670 OS=Cucurbita moschata OX=3662 GN=LOC111463907 PE=4 SV=1)

HSP 1 Score: 661 bits (1705), Expect = 3.84e-238
Identity = 326/359 (90.81%), Postives = 344/359 (95.82%), Query Frame = 0

Query: 37  MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 96
           MAD  VRFGIIG AEIARKV+RAIALAPNATLSAIGSR++EKASKFA DNGF+P+VKIYG
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 97  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 156
           SYEAVLDDPE+DAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNV+E DKILEACE+N
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 157 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 216
           GVQFMDGTMWMHNPRTA MKEFLSDA+QFGQLKSV+S+FSF ADADFLANDIRVKSDLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 217 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 276
           LGALGDAGWYCIRSILFAA+FELPKRVIA PNPVLNESGVILSCGASL+WDDGK+ATFHC
Sbjct: 181 LGALGDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 277 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 336
           SFLSNLTMD+TAIGTNG LHVNDFIIPYGEKEA+F T SKSGFTELVTGWEPLPSKHIVP
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 337 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVG 395
           TDLPQEA MVREFSHLVKEIKANGSKPEKKWP ISRKTQLV+DAVKASLD G +PVEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of MC04g1387 vs. ExPASy TrEMBL
Match: A0A6J1JMT8 (uncharacterized oxidoreductase At4g09670 OS=Cucurbita maxima OX=3661 GN=LOC111487263 PE=4 SV=1)

HSP 1 Score: 660 bits (1704), Expect = 5.45e-238
Identity = 326/359 (90.81%), Postives = 343/359 (95.54%), Query Frame = 0

Query: 37  MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 96
           MAD  VRFGIIG AEIARKV+RAIALAPNATLSAIGSR++EKASKFA DNGF+P+VKIYG
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 97  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 156
           SYEAVLDDPE+DAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNV+E DKILEACE+N
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 157 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 216
           GVQFMDGTMWMHNPRTA MKEFLSDA+QFGQLKSV+S+FSF ADADFLANDIRVKSDLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 217 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 276
           LGALGDAGWYCIRSILFAA FELPKRVIA PNPVLNESGVILSCGASL+WDDGK+ATFHC
Sbjct: 181 LGALGDAGWYCIRSILFAASFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 277 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 336
           SFLSNLTMD+TAIGTNG LHVNDFIIPYGEKEA+F T SKSGFTELVTGWEPLPSKHIVP
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 337 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVG 395
           TDLPQEA MVREFSHLVKEIKANGSKPEKKWP ISRKTQLV+DAVKASLD G +PVEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of MC04g1387 vs. ExPASy TrEMBL
Match: A0A0A0KB74 (GFO_IDH_MocA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G052640 PE=4 SV=1)

HSP 1 Score: 645 bits (1665), Expect = 4.59e-232
Identity = 318/360 (88.33%), Postives = 343/360 (95.28%), Query Frame = 0

Query: 37  MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 96
           MAD  +RFGIIG A+IARKV+RAIALAPNATLSAIGSR+LEKASKFASDNGF+P VKIYG
Sbjct: 1   MADTIIRFGIIGAADIARKVSRAIALAPNATLSAIGSRSLEKASKFASDNGFSPHVKIYG 60

Query: 97  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 156
           SYEAVLDDPE+DAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAE DKILEACE+N
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAEFDKILEACEAN 120

Query: 157 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 216
           GVQFMDGTMWMHNPRTAKMKEFLSDAD+FGQLK+V+S+FSF AD+DFLANDIRVK DLDG
Sbjct: 121 GVQFMDGTMWMHNPRTAKMKEFLSDADKFGQLKAVNSVFSFAADSDFLANDIRVKPDLDG 180

Query: 217 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 276
           LGALGDAGWYCIRSILFAA++ELPKRVIALPNPVLNE GVILSCG SL W+DGKVATFHC
Sbjct: 181 LGALGDAGWYCIRSILFAANYELPKRVIALPNPVLNELGVILSCGCSLLWEDGKVATFHC 240

Query: 277 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 336
           SFLSNLTMD+TAIGTNG LH+NDFIIPY EKEA+F+T SKSGF +LVTGW+PLPSKHIVP
Sbjct: 241 SFLSNLTMDVTAIGTNGTLHLNDFIIPYVEKEAYFSTCSKSGFNDLVTGWQPLPSKHIVP 300

Query: 337 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVGK 396
           TDLPQEA MVREFSHLVKEIK NGSKPEKKWPTI+RKTQLV+DAVKASLDRG EPVE+G+
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKLNGSKPEKKWPTITRKTQLVLDAVKASLDRG-EPVELGQ 359

BLAST of MC04g1387 vs. ExPASy TrEMBL
Match: A0A5A7SM23 (Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G00420 PE=4 SV=1)

HSP 1 Score: 643 bits (1658), Expect = 5.54e-231
Identity = 316/359 (88.02%), Postives = 340/359 (94.71%), Query Frame = 0

Query: 37  MADATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYG 96
           MAD  +RFGIIG A+IARKV+RAIALAPNATLSAIGSR+LEKASKFASDNGF+P VKIYG
Sbjct: 1   MADTIIRFGIIGAADIARKVSRAIALAPNATLSAIGSRSLEKASKFASDNGFSPHVKIYG 60

Query: 97  SYEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESN 156
           SYEAVLDDPE+DAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAE DKILEACE+N
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAEFDKILEACEAN 120

Query: 157 GVQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDG 216
           GVQFMDGTMWMHNPRTAKMKEFL DAD+FGQLK+V+S+FSF ADADFLANDIRVK DLDG
Sbjct: 121 GVQFMDGTMWMHNPRTAKMKEFLFDADKFGQLKAVNSVFSFAADADFLANDIRVKPDLDG 180

Query: 217 LGALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHC 276
           LGALGDAGWYCIRSILFAA++ELPKRVIALPNPVLNE GVILSCG SL W+DGKVATFHC
Sbjct: 181 LGALGDAGWYCIRSILFAANYELPKRVIALPNPVLNELGVILSCGCSLLWEDGKVATFHC 240

Query: 277 SFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVP 336
           SFLSNLTMD+TAIGTNG LH+NDFIIPY EKEA+F+T SKSGFTELVTGW+PLPSKHIVP
Sbjct: 241 SFLSNLTMDVTAIGTNGTLHLNDFIIPYVEKEAYFSTCSKSGFTELVTGWQPLPSKHIVP 300

Query: 337 TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVG 395
           TDLPQEA MVREFSHLVKEIK NG+KPE+KWPTI+RKTQLV+DAVKASLD G  PVE+G
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKLNGAKPEEKWPTITRKTQLVLDAVKASLDGGFVPVELG 359

BLAST of MC04g1387 vs. TAIR 10
Match: AT4G09670.1 (Oxidoreductase family protein )

HSP 1 Score: 493.0 bits (1268), Expect = 2.2e-139
Identity = 235/356 (66.01%), Postives = 289/356 (81.18%), Query Frame = 0

Query: 39  DATVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYGSY 98
           +  +R G++GCA+IARKV+RAI LAPNAT+S + SR+LEKA  FA+ N +    KI+GSY
Sbjct: 4   ETQIRIGVMGCADIARKVSRAIHLAPNATISGVASRSLEKAKAFATANNYPESTKIHGSY 63

Query: 99  EAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNGV 158
           E++L+DPE+DA+YVPLPTSLH+ WA+ AAEK KH+LLEKPVA+NV E DKI++ACE+NGV
Sbjct: 64  ESLLEDPEIDALYVPLPTSLHVEWAIKAAEKGKHILLEKPVAMNVTEFDKIVDACEANGV 123

Query: 159 QFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDGLG 218
           Q MDGTMW+HNPRTA +KEFLSD+++FGQLK+V S FSF  D DFL NDIRVK  LDGLG
Sbjct: 124 QIMDGTMWVHNPRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKNDIRVKPGLDGLG 183

Query: 219 ALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHCSF 278
           ALGDAGWY IR+ L A +FELPK V A P  VLNE+GVILSCGASL W+DG+ AT +CSF
Sbjct: 184 ALGDAGWYAIRATLLANNFELPKTVTAFPGAVLNEAGVILSCGASLSWEDGRTATIYCSF 243

Query: 279 LSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVPTD 338
           L+NLTM++TAIGT G L V+DFIIPY E EA FTT +K+ F +LVT W   PS+H V T+
Sbjct: 244 LANLTMEITAIGTKGTLRVHDFIIPYKETEASFTTSTKAWFNDLVTAWVSPPSEHTVKTE 303

Query: 339 LPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEV 395
           LPQEA MVREF+ LV EIK NG+KP+  WP+ISRKTQLVVDAVK S+D+  + + +
Sbjct: 304 LPQEACMVREFARLVGEIKNNGAKPDGYWPSISRKTQLVVDAVKESVDKNYQQISL 359

BLAST of MC04g1387 vs. TAIR 10
Match: AT1G34200.1 (Glyceraldehyde-3-phosphate dehydrogenase-like family protein )

HSP 1 Score: 410.6 bits (1054), Expect = 1.4e-114
Identity = 205/355 (57.75%), Postives = 259/355 (72.96%), Query Frame = 0

Query: 39  DATVRFGIIGCAEIARKVARAIALAPNATLSAIG-SRTLEKASKFASDNGFTPRVKIYGS 98
           D  +R G++ C    RK++RAI L+PNAT+SAI  + ++E+A  FA  N F P  K++ S
Sbjct: 4   DNQIRIGLLSCTNKVRKLSRAINLSPNATISAIATTSSIEEAKSFAKSNNFPPNTKLHSS 63

Query: 99  YEAVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNG 158
           YE++L+DP+VDAVY P+PT LH+ WA LAA K KH+LL+KPVALNVAE D+I+EACE NG
Sbjct: 64  YESLLEDPDVDAVYFPIPTRLHVEWATLAAIKGKHILLDKPVALNVAEFDQIVEACEVNG 123

Query: 159 VQFMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDGL 218
           VQFMDGT WMH+PRT K+KEF++D + FGQ+KSV+S FSF A  DFL +DIRVK  LDGL
Sbjct: 124 VQFMDGTQWMHSPRTDKIKEFVNDLESFGQIKSVYSCFSFAASDDFLKHDIRVKPGLDGL 183

Query: 219 GALGDAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHCS 278
           GALGDAGWY I++IL   +F+LPK V AL   VLN++G++LSCGA   W+DG  AT +CS
Sbjct: 184 GALGDAGWYTIQAILLVNNFKLPKTVTALSGSVLNDAGIVLSCGALFDWEDGVNATIYCS 243

Query: 279 FLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVPT 338
           FL+NLTM++TAIGT G+L V+DF+IPY E EA FTT                 S+H V T
Sbjct: 244 FLANLTMEITAIGTKGSLRVHDFVIPYMETEAVFTTSGGG-----------CVSEHKVMT 303

Query: 339 DLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPV 393
           +L QEA MV EF+ LV EIK  G+KP+  W   SR TQL+VDAVK S+D   EPV
Sbjct: 304 ELSQEACMVMEFARLVGEIKNRGAKPDGFWMRFSRNTQLLVDAVKESIDNNLEPV 347

BLAST of MC04g1387 vs. TAIR 10
Match: AT1G66130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 5.1e-80
Identity = 160/362 (44.20%), Postives = 226/362 (62.43%), Query Frame = 0

Query: 41  TVRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPR-VKIYGSYE 100
           TVRFG++GC   A K  R +  + NA + AI   +LE A  FA+ N  +P  V IYGSYE
Sbjct: 6   TVRFGLLGCIRFASKFVRTVTESSNAVIIAISDPSLETAKTFATGNNLSPETVTIYGSYE 65

Query: 101 AVLDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNGVQ 160
            +L+D  VDAVY+ +P +   RWAV AAEKKKH+L+EKP A +  EL+KI+EACE NGVQ
Sbjct: 66  ELLNDANVDAVYLTMPVTQRARWAVTAAEKKKHVLVEKPPAQDATELEKIVEACEYNGVQ 125

Query: 161 FMDGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDGL-G 220
           FMDGT+W+H+ RT K+++ + D+   G ++ ++S  +       L    R+  +  GL G
Sbjct: 126 FMDGTIWLHHQRTVKIRDTMFDSGLLGDVRHMYSTMTTPVPEQVLE---RLTKEAMGLAG 185

Query: 221 ALGDAGWYCIRSILFAADFELPKRVIALPNPV-LNESGVILSCGASLYWDDGKVAT--FH 280
           A+G+ GWY I + L+A  +++P  V ALP+ V  N  G ILSC ASL +      T   H
Sbjct: 186 AIGELGWYPIGAALWAMSYQMPISVRALPSSVSTNSVGTILSCTASLQFGSTATTTAIVH 245

Query: 281 CSFLSNLTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIV 340
           CSFLS L+ D+   G+ G++ +ND++IPY E +A F   S + F ++  GW   P +  V
Sbjct: 246 CSFLSQLSTDLAISGSKGSIQMNDYVIPYKEDKAWFEYTSGAKFVDMHIGWNVTPERVTV 305

Query: 341 P---TDLPQEARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPV 395
               T   QEA M+REF+ LV+ IK    + +++WP IS+KTQL+VDAVK S+D G E V
Sbjct: 306 DCSGTPETQEAMMLREFTRLVEGIKRGDLEADRRWPDISKKTQLIVDAVKKSVDIGCEVV 364

BLAST of MC04g1387 vs. TAIR 10
Match: AT4G17370.1 (Oxidoreductase family protein )

HSP 1 Score: 43.9 bits (102), Expect = 3.5e-04
Identity = 38/164 (23.17%), Postives = 74/164 (45.12%), Query Frame = 0

Query: 35  ADMADATV-RFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFAS---DNGFTP 94
           AD+ D+ + ++GI+G   + R+    +    +  L+ +        S+  +      F  
Sbjct: 2   ADVKDSVITKYGIVGIGMMGREHLINLHHLRDQGLAVVCIADPHPPSQLLAIELAQSFGW 61

Query: 95  RVKIYGSYEAVLDDPEVDAVYVPLPTSLH--LRWAVLAAEKKKHLLLEKPVALNVAELDK 154
            +K++  +E +L     D + V  P   H  +   ++   K  H+L+EKP+   VA+  +
Sbjct: 62  ELKVFSGHEELLKSELCDVIVVSSPNMTHHQILMDIINYSKPHHVLVEKPLCTTVADCKQ 121

Query: 155 ILEACESNGVQFMD-GTMWMHNPRTAKMKEFLSDADQFGQLKSV 192
           +LEA +      +  G  + + P  AK+ E +   D FG +K V
Sbjct: 122 VLEAAKKRSDMVVQVGLEYRYMPPVAKLIEQVKGRD-FGNVKMV 164

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZ833.1e-13866.01Uncharacterized oxidoreductase At4g09670 OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
P493052.6e-2829.86Uncharacterized oxidoreductase ORF334 OS=Rhizobium meliloti OX=382 PE=3 SV=1[more]
D4GP306.0e-2534.77D-xylose 1-dehydrogenase (NADP(+)) 2 OS=Haloferax volcanii (strain ATCC 29605 / ... [more]
Q9ZA334.7e-2232.75dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Streptomyces viola... [more]
B3TMR86.2e-2229.81dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Actinomadura kijan... [more]
Match NameE-valueIdentityDescription
XP_022134449.12.71e-261100.00uncharacterized oxidoreductase At4g09670-like [Momordica charantia][more]
XP_023540091.17.93e-23890.81uncharacterized oxidoreductase At4g09670 [Cucurbita pepo subsp. pepo][more]
XP_022963640.17.93e-23890.81uncharacterized oxidoreductase At4g09670 [Cucurbita moschata] >XP_022963647.1 un... [more]
XP_022990391.11.13e-23790.81uncharacterized oxidoreductase At4g09670 [Cucurbita maxima] >XP_022990392.1 unch... [more]
KAG6602218.16.51e-23790.25putative oxidoreductase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1BYS01.31e-261100.00uncharacterized oxidoreductase At4g09670-like OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1HFT33.84e-23890.81uncharacterized oxidoreductase At4g09670 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1JMT85.45e-23890.81uncharacterized oxidoreductase At4g09670 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A0A0KB744.59e-23288.33GFO_IDH_MocA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G05264... [more]
A0A5A7SM235.54e-23188.02Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT4G09670.12.2e-13966.01Oxidoreductase family protein [more]
AT1G34200.11.4e-11457.75Glyceraldehyde-3-phosphate dehydrogenase-like family protein [more]
AT1G66130.15.1e-8044.20NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G17370.13.5e-0423.17Oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.360.10Dihydrodipicolinate Reductase; domain 2coord: 169..395
e-value: 5.1E-70
score: 237.5
NoneNo IPR availableGENE3D3.40.50.720coord: 37..168
e-value: 7.6E-38
score: 131.8
NoneNo IPR availablePANTHERPTHR46368:SF13SUBFAMILY NOT NAMEDcoord: 37..394
NoneNo IPR availablePANTHERPTHR46368FAMILY NOT NAMEDcoord: 37..394
NoneNo IPR availableSUPERFAMILY55347Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domaincoord: 169..354
IPR000683Gfo/Idh/MocA-like oxidoreductase, N-terminalPFAMPF01408GFO_IDH_MocAcoord: 42..161
e-value: 7.1E-20
score: 72.0
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 37..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g1387.1MC04g1387.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000166 nucleotide binding