MC04g1166 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g1166 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 8.5e-243
Identity = 391/548 (71.35%), Postives = 473/548 (86.31%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSNG 67
           MEDLKPRPA+SSPLTPLGFL+RAAT+YGDC SV+Y + +YTWS THRRCL LASS++S G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 68  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQA 127
           I  GHVVSV+APN P MYEL FAVPMAGAILN +NLRLDA TIS+LL HSESKLIFVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 128 SCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFGDFVDTYEGMVEKGDPEFRWIR 187
           S  LI +A+ LFP ++  P LV +AD+ +   +SS +  +F  +Y+ ++++GDP+F+W+ 
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADE-SESGNSSELGKEFFCSYKDLIDRGDPDFKWVM 180

Query: 188 PLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMFHGNG 247
           P SEWDP++LNYTSGTTSSPKGVVHCHRGIF++T+DSL++WG+PKQ VYLWTLPMFH NG
Sbjct: 181 PKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFHANG 240

Query: 248 WCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPLDRPV 307
           W + WGMAAVGGTN+CLRKFD+  I+++I+ HGVTHMCGAPVVLNML+N+P ++PL   V
Sbjct: 241 WSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLKTTV 300

Query: 308 KILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERARLKAR 367
           +I+TAGAPPP+ VLFRTESLGF VSH YGLTET GLVVSC+WK +WN LPATERARLK+R
Sbjct: 301 QIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARLKSR 360

Query: 368 QGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTDDGWF 427
           QGV T    ++DVVDP T  +VKRDGS++GE+V+RG ++MLGYLKDPE T+KSMT DGWF
Sbjct: 361 QGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTADGWF 420

Query: 428 FTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPDEFWG 487
           +TGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVES+LY++P + EAAVVARPDEFWG
Sbjct: 421 YTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDEFWG 480

Query: 488 ETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKFGLRK 547
           ETPCAFVSL++GLT+KPTE+EI+EYCR KLPR+MVPKTVVF  ELPKTSTGK++KF LR 
Sbjct: 481 ETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFILRD 540

Query: 548 IAKSMGPA 556
           +A+ MG A
Sbjct: 541 MARGMGSA 547

BLAST of MC04g1166 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 9.1e-229
Identity = 382/557 (68.58%), Postives = 458/557 (82.23%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGS-TTYTWSHTHRRCLRLASSLSSN 67
           ME +KP  ANS PLTP+GFL+RAAT+YGDC S++YGS T YTW  T+ RCLR+ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 68  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQ 127
           GI R  VVSV++PNTPAMYELQFAVPM+GAILNNIN RLDA T+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 128 ASCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFGD---FVDTYEGMVEKGDPEF 187
            S  L  +A+++  T    P+LV IAD    EE       D   F  TY+ ++ +GD +F
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180

Query: 188 RWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMF 247
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF++++DSL++W +PK  VYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240

Query: 248 HGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPL 307
           H NGW + WG+AAVGGTNVCLRKFDAP I+ LIR HGVTHMCGAPVVLNML+ + + QPL
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
           +RPV ILTAGAPPPA VL R ES+GF +SH YGLTET GL VSC+WKPQWNRLPA++RAR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LKARQGVRT    E+DVVDPE+ +SV+R+G ++GEIVMRG +IMLGYLKDP  T K++  
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-K 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           +GWF+TGD+GV+H+DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
            FWGETPCAFVSL+ GLT++PTE E+IEYCR K+P++MVPKTV F  ELPKTSTGK+ KF
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540

Query: 548 GLRKIAKSMGPAHTSHL 561
            LR+IAK MG    S +
Sbjct: 541 VLREIAKKMGTTRLSRM 552

BLAST of MC04g1166 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 2.2e-227
Identity = 377/550 (68.55%), Postives = 457/550 (83.09%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGS-TTYTWSHTHRRCLRLASSLSSN 67
           ME++KP  ANS PLTP+GFL+RAAT+YGDC S++YGS T YTW  T+ RCLR+ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 68  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQ 127
           GI R  VVSV++PNTPAMYELQFAVPM+GAILNNIN RLDA T+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 128 ASCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFGD---FVDTYEGMVEKGDPEF 187
            S  L  +A+++  T    P+LV+IAD    EE       D   F  TY+ ++E+GDP F
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180

Query: 188 RWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMF 247
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF++++DSL++W +PK  VYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 248 HGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPL 307
           H NGW   WG+AAVGGTNVCLRKFDAP I+ LIR HGVTHMCGAPVVLNML+ + ++QPL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
           + PV ILTAG+PPPATVL R ES+GF +SH YGLTET G++VSC+WKP+WN LPA++RAR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LKARQGVRT    E+DVVDPE+  SV+R+G ++GEIVMRG ++MLGYLKDP  T K++  
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-K 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           +GWF+TGD+GV+H+DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
           EFWGETPCAFVSL+ G + KPTEEE++EYCR K+P++MVPKTV F  ELPK+STGK+ KF
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 548 GLRKIAKSMG 554
            LR IAK MG
Sbjct: 541 VLRDIAKKMG 545

BLAST of MC04g1166 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 3.8e-227
Identity = 378/553 (68.35%), Postives = 458/553 (82.82%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSS-N 67
           ME+LKPR ANS PLTPLGFL+RAAT+YGDC SV+Y   +YTWS TH RCLR+AS + S  
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 68  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQ 127
           G+ +G VVSVVAPN PAMYEL FAVPMAG +LNNIN RL+A TIS++LRHSESKL+FVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 128 ASCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFGDFVDTYEGMVEKGDPE-FRW 187
            S  L+ DA++LFP  +  P+LVLIAD++  E  +      FV  YE +VEKGD + F+W
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGENLTVEDREHFVCCYEDLVEKGDDKTFKW 180

Query: 188 IRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMFHG 247
           +RP+SEWDPIVLNYTSGTTSSPKGVVH HR +F+VTLDSL++WG+PKQ VYLWTLPMFH 
Sbjct: 181 VRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPMFHA 240

Query: 248 NGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPLDR 307
           NGW ++WG+AAVGGTN+CLR+FD   IF LIR H VTHMC AP+VLNML+NSP+ +PL  
Sbjct: 241 NGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEPLPN 300

Query: 308 PVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERARLK 367
           PV ++T GAPPPA VL RTESLGF +SH YG+TE  G+VVSC+WK +WNRLPATE+ARLK
Sbjct: 301 PVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQARLK 360

Query: 368 ARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTDDG 427
           +RQGVRTA M EVDVVDP +  SVKRDG ++GEIV++G +IMLGYLK+  AT+K +  DG
Sbjct: 361 SRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIRADG 420

Query: 428 WFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPDEF 487
           WF+TGD+ VMH DGYLEIKDRSKDVIISGGEN+SSVEVES LY++PAV+EAAVVA PDE+
Sbjct: 421 WFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACPDEY 480

Query: 488 WGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKFGL 547
           WGETP AFV+L++G+  +PTE+EI+EYCR KL  FMVPK VVF  +LPKTSTGKI+KF L
Sbjct: 481 WGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQKFTL 540

Query: 548 RKIAKSMGPAHTS 559
           ++I K+MG + +S
Sbjct: 541 KEIVKTMGYSSSS 553

BLAST of MC04g1166 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 6.1e-217
Identity = 370/549 (67.40%), Postives = 446/549 (81.24%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYG-STTYTWSHTHRRCLRLASSLSSN 67
           MEDLKP  ANS PLT LGFL+RAAT+YGDC S++YG ST YTW  T+ RCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 68  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQ 127
           GI R  VVSV++ NTP MYELQF+VPM+GAILNNIN RLDA T+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 128 ASCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIF--GDFVDTYEGMVEKGDPEFR 187
               L  +A+ +       P+LVLIA++   EE  + +     F   Y  ++ +G+P+F+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANE-EEEEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 188 WIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMFH 247
           WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRGIF++TLDSL +W +PK  VYLWTLP+FH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 248 GNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPD-NQPL 307
            NGW + WG+AAVGGTNVC+RK  APSI+ LIR HGVTHM GAP+VL +L+ S + +QPL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
             PV  LTAG+ PPATVL R ESLGF VSH YGLTET G++VSC+WKP WNRLPA+++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LK+RQGVRT   +E+DVVDPE+ +SV+RDG ++GEIV+RG +IMLGYLK+P  T  S   
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           +GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPD
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
           EFWGETPCAFVSL+ GLTRKPT++EIIEYC+ K+PR+M PKTV F  ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 548 GLRKIAKSM 553
            L++IAK+M
Sbjct: 541 LLKEIAKNM 544

BLAST of MC04g1166 vs. NCBI nr
Match: XP_038877068.1 (probable acyl-activating enzyme 5, peroxisomal [Benincasa hispida])

HSP 1 Score: 937 bits (2423), Expect = 0.0
Identity = 458/565 (81.06%), Postives = 506/565 (89.56%), Query Frame = 0

Query: 1   TVSVTISMEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLA 60
           T+S +ISMEDLKP  ANSSPLTP+GFL+RA+ IYGD PS+IY STTYTWS T+RRCLR+A
Sbjct: 21  TISQSISMEDLKPSLANSSPLTPIGFLERASVIYGDSPSIIYNSTTYTWSETYRRCLRVA 80

Query: 61  SSLSSNGIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESK 120
           SSLSS GI +G+VVSV+APNTP MYELQFAVPMAGA+LN INLRLDA TIS+LLRH ESK
Sbjct: 81  SSLSSIGIQKGYVVSVIAPNTPPMYELQFAVPMAGAVLNCINLRLDARTISILLRHCESK 140

Query: 121 LIFVDQASCSLINDALNLFPTKSKRPVLVLIADDVATEES-----SSPIFGDFVDTYEGM 180
           LIF+DQASC LI+DAL LFP ++KRP+LVLIADD A E+S     SS I G+FVD+YE M
Sbjct: 141 LIFIDQASCGLISDALALFPPETKRPLLVLIADDTAVEDSLSSSSSSVIGGEFVDSYEEM 200

Query: 181 VEKGDPEFRWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSV 240
           V+KGD EFRWIRP+SEWDPIV+NYTSGTTSSPKGVVH HRGIF VTLDSL+EWG+PKQSV
Sbjct: 201 VQKGDDEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFAVTLDSLIEWGVPKQSV 260

Query: 241 YLWTLPMFHGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLT 300
           YLWTLPMFH NGW F +G+AAVGGTNVC+RKFDA  IF LI  H VTH+CGAPVVLNMLT
Sbjct: 261 YLWTLPMFHANGWSFPFGIAAVGGTNVCIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLT 320

Query: 301 NSPDNQPLDRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNR 360
           NSPDNQPL+RPVKILTAGAPPPA VLFRTESLGFDV H YGLTETGGLV+ CSWK +WNR
Sbjct: 321 NSPDNQPLERPVKILTAGAPPPAAVLFRTESLGFDVGHGYGLTETGGLVIYCSWKSKWNR 380

Query: 361 LPATERARLKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPE 420
           LPATERARLKARQGVRT  M+EVDV+D ET KSVKRDG+SIGEIV+RG ++MLGY KDPE
Sbjct: 381 LPATERARLKARQGVRTLAMSEVDVLDSETGKSVKRDGASIGEIVLRGGSMMLGYFKDPE 440

Query: 421 ATSKSMTDDGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNE 480
           AT K+MTD+GWFFTGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVESVLYTNPAVNE
Sbjct: 441 ATKKAMTDEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNE 500

Query: 481 AAVVARPDEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKT 540
           AAVVARPDEFWGETPCAFVSLREGLTR PTEEEI+EYCR KLP+FMVPKTVVF  +LPKT
Sbjct: 501 AAVVARPDEFWGETPCAFVSLREGLTRLPTEEEIMEYCREKLPKFMVPKTVVFMEKLPKT 560

Query: 541 STGKIEKFGLRKIAKSMGPAHTSHL 560
           STGKI+KF LR+ AKSMGPA TS L
Sbjct: 561 STGKIQKFLLREKAKSMGPARTSRL 585

BLAST of MC04g1166 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 453/557 (81.33%), Postives = 497/557 (89.23%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSNG 67
           MEDLKP PANSSPLTP+GFL+RAA +YGDCPS+IY STTYTW+ THRRCL+LASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 68  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQA 127
           I RG VVSVV+PNT AMYEL FAVPMAGA+LN INLRLDA TIS++LRH E+ LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 128 SCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIF----GDFVDTYEGMVEKGDPEF 187
           SC LI+DAL LFP + KRP L+LIAD+ A E+SSS        DFVD+YE MV+KGDP F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 188 RWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMF 247
           +WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF VTLDSL+EWG+PK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 248 HGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPL 307
           H NGW F WG+AAVGGTNVC+RKFDA +IF LI  + VTH+CGAPVVLNMLTNSPDN+PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
           DRPVKILTAGAPPPA VLFRTESLGFDVSH YGLTETGGLV+ CSWK  WNRLPA ERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LKARQGVRT  MA  DV+DPET KSVKRDG+SIGEIV+RG ++MLGYLKDPEAT+K+MT 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           +GWFFTGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
           EFWGETPCAFVSL+EGLTR+PTE EIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKI+KF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 548 GLRKIAKSMGPAHTSHL 560
            LR++AKSMGPA  S L
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of MC04g1166 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 928 bits (2399), Expect = 0.0
Identity = 451/561 (80.39%), Postives = 496/561 (88.41%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSNG 67
           MEDLKP PANSSPLTP+GFL+RAA +YGDCPS+IY STTYTW+ THRRCL+LASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 68  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQA 127
           I RG VVSVV+PNTPAMYEL FAVPMAGA+LN INLRLD+ TIS++LRH E+ LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 128 SCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFG--------DFVDTYEGMVEKG 187
           SC LI+DAL LFP + KRP L+LIAD+ A E+SSS            DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 188 DPEFRWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWT 247
           DP F+WIRP+SEWDPIVLNYTSGTTSSPKGVVHCHRGIF VTLDSL+EWG+ KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 248 LPMFHGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPD 307
           LPMFH NGW F WG+AAVGGTNVC+RKFDA +IF LI  + VTH+CGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 308 NQPLDRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPAT 367
           N+PLDRPVKILTAGAPPPA VLFRTESLGFDVSH YGLTETGGLV+ CSWK  WNRLPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 368 ERARLKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSK 427
           ERARLKARQGVRT  MA  DV+ PET KSVKRDG+SIGEIV+RG ++MLGYLKDPEAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 428 SMTDDGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVV 487
           +MT +GWFFTGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 488 ARPDEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 547
           ARPDEFWGETPCAFVSL+EGLTR+PTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 548 IEKFGLRKIAKSMGPAHTSHL 560
           I+KF LR++AKSMGPA  S L
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of MC04g1166 vs. NCBI nr
Match: XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 928 bits (2399), Expect = 0.0
Identity = 451/561 (80.39%), Postives = 496/561 (88.41%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSNG 67
           MEDLKP PANSSPLTP+GFL+RAA +YGDC S+IY STTYTW+ THRRCL+LASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60

Query: 68  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQA 127
           I RG VVSVV+PNT AMYEL FAVPMAGA+LN INLRLDA TIS++LRH E+ LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 128 SCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFG--------DFVDTYEGMVEKG 187
           SC LI+DAL LFP + KRP L+LIAD+ A E+SSS            DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180

Query: 188 DPEFRWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWT 247
           DP F+WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF VTLDSL+EWG+PK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240

Query: 248 LPMFHGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPD 307
           LPMFH NGW F WG+AAVGGTNVC+RKFDA +IF LI  + VTH+CGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 308 NQPLDRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPAT 367
           N+PLDRPVKILTAGAPPPA VLFRTESLGFDVSH YGLTETGGLV+ CSWK  WNRLPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 368 ERARLKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSK 427
           +RARLKARQGVRT  MA  DV+DPET KSVKRDG+SIGEIV+RG ++MLGYLKDPEAT+K
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 428 SMTDDGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVV 487
           +MT +GWFFTGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 488 ARPDEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 547
           ARPDEFWGETPCAFVSL+EGLTR+PTE EIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540

Query: 548 IEKFGLRKIAKSMGPAHTSHL 560
           I+KF LR++AKSMGPA  S L
Sbjct: 541 IQKFVLREMAKSMGPAGKSRL 561

BLAST of MC04g1166 vs. NCBI nr
Match: XP_008463174.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046220.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18976.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 910 bits (2353), Expect = 0.0
Identity = 437/557 (78.46%), Postives = 491/557 (88.15%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSNG 67
           MEDLKP PANS PLTP+GF+ RAATIYGD  S+IY STTYTWS THRRCL+LASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 68  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQA 127
           I RGHVVSV+APNTPAMYEL FAVPM GAILN INLRLD  TIS+LLRHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 128 SCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIF----GDFVDTYEGMVEKGDPEF 187
           SC+LI +AL LFP + KRP+LVLIADD A ++SS P F     +FVD+YE MV++GD +F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 188 RWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMF 247
           +WIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIF V LDSLLEWG+PKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 248 HGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPL 307
           HGNGW F WG+AAVGGTNVC+RKFDA  IF LIR H VTH+CGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
           DRPVKILTAGAPPPA++L RTESLGF V+H YGLTE+ G ++ CSWK +WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LKARQGVR   +  VDV+DPET KSVKRDG+SIGEIV+RG ++ LGYLKDPE T+K+M++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           DGWFFTGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
           EFWGETPCAF+SL+EGL+RKPTEEEIIEYC+GKLPRFMVPKTV+F  E+PKT+TGKI+KF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 548 GLRKIAKSMGPAHTSHL 560
            LR+ AKS+GPA  S L
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of MC04g1166 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 453/557 (81.33%), Postives = 497/557 (89.23%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSNG 67
           MEDLKP PANSSPLTP+GFL+RAA +YGDCPS+IY STTYTW+ THRRCL+LASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 68  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQA 127
           I RG VVSVV+PNT AMYEL FAVPMAGA+LN INLRLDA TIS++LRH E+ LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 128 SCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIF----GDFVDTYEGMVEKGDPEF 187
           SC LI+DAL LFP + KRP L+LIAD+ A E+SSS        DFVD+YE MV+KGDP F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 188 RWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMF 247
           +WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF VTLDSL+EWG+PK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 248 HGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPL 307
           H NGW F WG+AAVGGTNVC+RKFDA +IF LI  + VTH+CGAPVVLNMLTNSPDN+PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
           DRPVKILTAGAPPPA VLFRTESLGFDVSH YGLTETGGLV+ CSWK  WNRLPA ERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LKARQGVRT  MA  DV+DPET KSVKRDG+SIGEIV+RG ++MLGYLKDPEAT+K+MT 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           +GWFFTGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
           EFWGETPCAFVSL+EGLTR+PTE EIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKI+KF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 548 GLRKIAKSMGPAHTSHL 560
            LR++AKSMGPA  S L
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of MC04g1166 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 928 bits (2399), Expect = 0.0
Identity = 451/561 (80.39%), Postives = 496/561 (88.41%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSNG 67
           MEDLKP PANSSPLTP+GFL+RAA +YGDCPS+IY STTYTW+ THRRCL+LASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 68  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQA 127
           I RG VVSVV+PNTPAMYEL FAVPMAGA+LN INLRLD+ TIS++LRH E+ LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 128 SCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFG--------DFVDTYEGMVEKG 187
           SC LI+DAL LFP + KRP L+LIAD+ A E+SSS            DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 188 DPEFRWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWT 247
           DP F+WIRP+SEWDPIVLNYTSGTTSSPKGVVHCHRGIF VTLDSL+EWG+ KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 248 LPMFHGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPD 307
           LPMFH NGW F WG+AAVGGTNVC+RKFDA +IF LI  + VTH+CGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 308 NQPLDRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPAT 367
           N+PLDRPVKILTAGAPPPA VLFRTESLGFDVSH YGLTETGGLV+ CSWK  WNRLPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 368 ERARLKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSK 427
           ERARLKARQGVRT  MA  DV+ PET KSVKRDG+SIGEIV+RG ++MLGYLKDPEAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 428 SMTDDGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVV 487
           +MT +GWFFTGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 488 ARPDEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 547
           ARPDEFWGETPCAFVSL+EGLTR+PTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 548 IEKFGLRKIAKSMGPAHTSHL 560
           I+KF LR++AKSMGPA  S L
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of MC04g1166 vs. ExPASy TrEMBL
Match: A0A5D3D623 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00690 PE=4 SV=1)

HSP 1 Score: 910 bits (2353), Expect = 0.0
Identity = 437/557 (78.46%), Postives = 491/557 (88.15%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSNG 67
           MEDLKP PANS PLTP+GF+ RAATIYGD  S+IY STTYTWS THRRCL+LASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 68  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQA 127
           I RGHVVSV+APNTPAMYEL FAVPM GAILN INLRLD  TIS+LLRHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 128 SCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIF----GDFVDTYEGMVEKGDPEF 187
           SC+LI +AL LFP + KRP+LVLIADD A ++SS P F     +FVD+YE MV++GD +F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 188 RWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMF 247
           +WIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIF V LDSLLEWG+PKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 248 HGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPL 307
           HGNGW F WG+AAVGGTNVC+RKFDA  IF LIR H VTH+CGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
           DRPVKILTAGAPPPA++L RTESLGF V+H YGLTE+ G ++ CSWK +WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LKARQGVR   +  VDV+DPET KSVKRDG+SIGEIV+RG ++ LGYLKDPE T+K+M++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           DGWFFTGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
           EFWGETPCAF+SL+EGL+RKPTEEEIIEYC+GKLPRFMVPKTV+F  E+PKT+TGKI+KF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 548 GLRKIAKSMGPAHTSHL 560
            LR+ AKS+GPA  S L
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of MC04g1166 vs. ExPASy TrEMBL
Match: A0A1S3CIL0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501381 PE=4 SV=1)

HSP 1 Score: 910 bits (2353), Expect = 0.0
Identity = 437/557 (78.46%), Postives = 491/557 (88.15%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSNG 67
           MEDLKP PANS PLTP+GF+ RAATIYGD  S+IY STTYTWS THRRCL+LASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 68  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQA 127
           I RGHVVSV+APNTPAMYEL FAVPM GAILN INLRLD  TIS+LLRHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 128 SCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIF----GDFVDTYEGMVEKGDPEF 187
           SC+LI +AL LFP + KRP+LVLIADD A ++SS P F     +FVD+YE MV++GD +F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 188 RWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMF 247
           +WIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIF V LDSLLEWG+PKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 248 HGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPL 307
           HGNGW F WG+AAVGGTNVC+RKFDA  IF LIR H VTH+CGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
           DRPVKILTAGAPPPA++L RTESLGF V+H YGLTE+ G ++ CSWK +WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LKARQGVR   +  VDV+DPET KSVKRDG+SIGEIV+RG ++ LGYLKDPE T+K+M++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           DGWFFTGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
           EFWGETPCAF+SL+EGL+RKPTEEEIIEYC+GKLPRFMVPKTV+F  E+PKT+TGKI+KF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 548 GLRKIAKSMGPAHTSHL 560
            LR+ AKS+GPA  S L
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of MC04g1166 vs. ExPASy TrEMBL
Match: A0A5A7TXV0 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00770 PE=4 SV=1)

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 439/555 (79.10%), Postives = 494/555 (89.01%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSNG 67
           M+ LKP PANSSPLTP+ FL+RA+ +Y D PS+IY ST++TWS T+ RCLRLASSLSS G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 68  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQA 127
           IH+GHVVSV++PNTP MYELQF+VPMAGAIL+ INLRLDA TIS+ LRHSESKL+FVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 128 SCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIF--GDFVDTYEGMVEKGDPEFRW 187
           S +LI+DAL LFP +SKRP+LVLIADD A E+S+S +   GDFVD YE M++KGDPEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 188 IRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMFHG 247
           IRP+SEWDPIV+NYTSGTTSSPKGVVH HRGIF VTLDSL+EWG+PK SVYLWTLPMFH 
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 248 NGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPLDR 307
           NGW F + +AAVGGTN+C+RKFDA  IF LI  H VTH+CGAPVVLNMLTNSP+N+PL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 308 PVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERARLK 367
           PVKILTAGAPPPA VLFRTESLGFDVSH YGLTETGGLV+ CSWK +WN+LPATERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 368 ARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTDDG 427
           ARQGVRT  MAE DV+D ET KSVKRDG SIGEIV+RG ++MLGY KDPEAT K+MT++G
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 428 WFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPDEF 487
           WFFTGD+GVMH DGYLEIKDRSKDVIISGGEN+SSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 488 WGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKFGL 547
           WGETPCAFVSLREGL+R PTEEEIIEYCRGKL  FMVPKTVVF  +LPKTSTGKI+KF L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 548 RKIAKSMGPAHTSHL 560
           R++AKSMGP+  S L
Sbjct: 541 REMAKSMGPSRKSRL 555

BLAST of MC04g1166 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 794.3 bits (2050), Expect = 6.5e-230
Identity = 382/557 (68.58%), Postives = 458/557 (82.23%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGS-TTYTWSHTHRRCLRLASSLSSN 67
           ME +KP  ANS PLTP+GFL+RAAT+YGDC S++YGS T YTW  T+ RCLR+ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 68  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQ 127
           GI R  VVSV++PNTPAMYELQFAVPM+GAILNNIN RLDA T+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 128 ASCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFGD---FVDTYEGMVEKGDPEF 187
            S  L  +A+++  T    P+LV IAD    EE       D   F  TY+ ++ +GD +F
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180

Query: 188 RWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMF 247
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF++++DSL++W +PK  VYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240

Query: 248 HGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPL 307
           H NGW + WG+AAVGGTNVCLRKFDAP I+ LIR HGVTHMCGAPVVLNML+ + + QPL
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
           +RPV ILTAGAPPPA VL R ES+GF +SH YGLTET GL VSC+WKPQWNRLPA++RAR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LKARQGVRT    E+DVVDPE+ +SV+R+G ++GEIVMRG +IMLGYLKDP  T K++  
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-K 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           +GWF+TGD+GV+H+DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
            FWGETPCAFVSL+ GLT++PTE E+IEYCR K+P++MVPKTV F  ELPKTSTGK+ KF
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540

Query: 548 GLRKIAKSMGPAHTSHL 561
            LR+IAK MG    S +
Sbjct: 541 VLREIAKKMGTTRLSRM 552

BLAST of MC04g1166 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 789.6 bits (2038), Expect = 1.6e-228
Identity = 377/550 (68.55%), Postives = 457/550 (83.09%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGS-TTYTWSHTHRRCLRLASSLSSN 67
           ME++KP  ANS PLTP+GFL+RAAT+YGDC S++YGS T YTW  T+ RCLR+ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 68  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQ 127
           GI R  VVSV++PNTPAMYELQFAVPM+GAILNNIN RLDA T+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 128 ASCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFGD---FVDTYEGMVEKGDPEF 187
            S  L  +A+++  T    P+LV+IAD    EE       D   F  TY+ ++E+GDP F
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180

Query: 188 RWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMF 247
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF++++DSL++W +PK  VYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 248 HGNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPL 307
           H NGW   WG+AAVGGTNVCLRKFDAP I+ LIR HGVTHMCGAPVVLNML+ + ++QPL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
           + PV ILTAG+PPPATVL R ES+GF +SH YGLTET G++VSC+WKP+WN LPA++RAR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LKARQGVRT    E+DVVDPE+  SV+R+G ++GEIVMRG ++MLGYLKDP  T K++  
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-K 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           +GWF+TGD+GV+H+DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
           EFWGETPCAFVSL+ G + KPTEEE++EYCR K+P++MVPKTV F  ELPK+STGK+ KF
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 548 GLRKIAKSMG 554
            LR IAK MG
Sbjct: 541 VLRDIAKKMG 545

BLAST of MC04g1166 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 755.0 bits (1948), Expect = 4.3e-218
Identity = 370/549 (67.40%), Postives = 446/549 (81.24%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYG-STTYTWSHTHRRCLRLASSLSSN 67
           MEDLKP  ANS PLT LGFL+RAAT+YGDC S++YG ST YTW  T+ RCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 68  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVDQ 127
           GI R  VVSV++ NTP MYELQF+VPM+GAILNNIN RLDA T+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 128 ASCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIF--GDFVDTYEGMVEKGDPEFR 187
               L  +A+ +       P+LVLIA++   EE  + +     F   Y  ++ +G+P+F+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANE-EEEEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 188 WIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMFH 247
           WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRGIF++TLDSL +W +PK  VYLWTLP+FH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 248 GNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPD-NQPL 307
            NGW + WG+AAVGGTNVC+RK  APSI+ LIR HGVTHM GAP+VL +L+ S + +QPL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 308 DRPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERAR 367
             PV  LTAG+ PPATVL R ESLGF VSH YGLTET G++VSC+WKP WNRLPA+++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 368 LKARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTD 427
           LK+RQGVRT   +E+DVVDPE+ +SV+RDG ++GEIV+RG +IMLGYLK+P  T  S   
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 428 DGWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPD 487
           +GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPD
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 488 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKF 547
           EFWGETPCAFVSL+ GLTRKPT++EIIEYC+ K+PR+M PKTV F  ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 548 GLRKIAKSM 553
            L++IAK+M
Sbjct: 541 LLKEIAKNM 544

BLAST of MC04g1166 vs. TAIR 10
Match: AT1G77240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 737.3 bits (1902), Expect = 9.4e-213
Identity = 352/547 (64.35%), Postives = 439/547 (80.26%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIYGSTTYTWSHTHRRCLRLASSLSSN- 67
           ME L P  +NS PLT LGFL+RAA+++GD PS+++ +T +TWS TH RCLR+AS+LSS  
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 68  -GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFVD 127
            GI+RG VVSV+ PN P++YELQFAVPM+GA+LNNIN RLDAH +S+LLRHSESKL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 128 QASCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFGDFVDTYEGMVEKGDPEFRW 187
             S SL+ +A++  P K +RP LV++ D      SSS    DF+DTYEG +E+GD  F+W
Sbjct: 121 HHSSSLVLEAVSFLP-KDERPRLVILNDGNDMPSSSSADM-DFLDTYEGFMERGDLRFKW 180

Query: 188 IRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMFHG 247
           +RP SEW P+VLNYTSGTTSSPKGVVH HR +FM T++SLL+W +P + VYLWTLPMFH 
Sbjct: 181 VRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLPMFHA 240

Query: 248 NGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPLDR 307
           NGW ++W  AAVG  N+C+ + D P+IF LI  + VTHMC AP+VLNMLTN P  +PL  
Sbjct: 241 NGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQKPLQS 300

Query: 308 PVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERARLK 367
           PVK++TAGAPPPATV+ + E+LGFDVSH YG+TETGGLVVSC+ KP+W+RL   ERA+ K
Sbjct: 301 PVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDERAKQK 360

Query: 368 ARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTDDG 427
           +RQG+RTA  AEVDV DP + KSVK DG+++GEIV RG ++MLGY KDPE T+ SM +DG
Sbjct: 361 SRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASMREDG 420

Query: 428 WFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPDEF 487
           WF+TGDIGVMH DGYLE+KDRSKDV+I GGENISS E+E+VLYTNPA+ EAAVVA+PD+ 
Sbjct: 421 WFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKPDKM 480

Query: 488 WGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIEKFGL 547
           WGETPCAFVSL+       TE EI E+C+ KLP++MVP+ VVF  ELPKTSTGKI+KF L
Sbjct: 481 WGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQKFLL 540

Query: 548 RKIAKSM 553
           R++AKS+
Sbjct: 541 RQMAKSL 544

BLAST of MC04g1166 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 735.7 bits (1898), Expect = 2.7e-212
Identity = 352/553 (63.65%), Postives = 442/553 (79.93%), Query Frame = 0

Query: 8   MEDLKPRPANSSPLTPLGFLDRAATIYGDCPSVIY-GSTTYTWSHTHRRCLRLASSLSSN 67
           ME L P P+NS+PLT LGFLDRAA++YGDCPS+++  +T +TWS TH RCLR+AS+L+S+
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 68  --GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNINLRLDAHTISMLLRHSESKLIFV 127
             GI+RG VVSVV PN P++YELQFAVPM+GAILNNIN RLDAH +S+LLRHSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 128 DQASCSLINDALNLFPTKSKRPVLVLIADDVATEESSSPIFGDFVDTYEGMVEKGDPEFR 187
           D  S S++ +A++ F  ++++P LVL+ DD      S     DF+DTY+G++E+GD  F+
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDDQEDGSLSPSAASDFLDTYQGVMERGDSRFK 180

Query: 188 WIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFMVTLDSLLEWGIPKQSVYLWTLPMFH 247
           WIRP +EW P++LNYTSGTTSSPKGVV  HR IFM+T+ SLL+W  P + VYLWTLPMFH
Sbjct: 181 WIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPMFH 240

Query: 248 GNGWCFSWGMAAVGGTNVCLRKFDAPSIFELIRTHGVTHMCGAPVVLNMLTNSPDNQPLD 307
            NGW ++WG AAVG TNVC R+ DAP+I++LI  H VTHMC AP+VLNMLTN P  +PL 
Sbjct: 241 ANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKPLK 300

Query: 308 RPVKILTAGAPPPATVLFRTESLGFDVSHVYGLTETGGLVVSCSWKPQWNRLPATERARL 367
            PV+++TAGAPPPA ++ R E+LGF+V H YGLTETGG VVSC+WK +W+ L   ERARL
Sbjct: 301 NPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERARL 360

Query: 368 KARQGVRTATMAEVDVVDPETCKSVKRDGSSIGEIVMRGVTIMLGYLKDPEATSKSMTDD 427
           K+RQGVRT   AEVDV DP T KSV+ DG S+GEIV++G ++MLGY KDPE T+  M +D
Sbjct: 361 KSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMRED 420

Query: 428 GWFFTGDIGVMHADGYLEIKDRSKDVIISGGENISSVEVESVLYTNPAVNEAAVVARPDE 487
           GWF++GD+GV+H DGYLE+KDRSKDVII GGENISS EVE+VLYTNP V EAAVVA+PD+
Sbjct: 421 GWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKPDK 480

Query: 488 FWGETPCAFVSLR-----EGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 547
            WGETPCAFVSL+      GL    TE EI E+C+ +LP++MVP+ V+F  ELPKTSTGK
Sbjct: 481 MWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKTSTGK 540

Query: 548 IEKFGLRKIAKSM 553
           I+KF LR++AKS+
Sbjct: 541 IQKFLLRQMAKSL 549