MC04g1120 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g1120
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionCytochrome b-c1 complex subunit Rieske, mitochondrial
LocationMC04: 19266373 .. 19271029 (+)
RNA-Seq ExpressionMC04g1120
SyntenyMC04g1120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTGCAATAAAATGAAAAGAAAAAAAATGTTCCACGCCTTAAGCAAAAAGGGACCGGGGGTCAAAATTCTGTAAGCTTCATATACCTTCGAATTCTGTCGCCTCCTCCTCCCTCTGAATTCTCAACACACCTCGAAGCTCCGATTCCCAAAACATTTTTCACGACGGAAGCTTTCGATTTGTAGACGAAGATGTTGAGGATTGCGGCTCGAAGGCTCTCTTCACTCTCTTCTTCCCATTTTAGGCCCACTCTCGCTTCTTCCTTCTCCGGTTCTCAGAAGATCGCTGATTCCGCTGATTCCGTCCCTTCCAATGATTTCAGATCCGTCGCCTCCTTCAACACCTTCCCTTTCGGTCTTCACTTTGATCGTAATTGCAGAGGTTAGAGTCACTTTTTTTTTTGTCCTGAGCTTTTGTCGTTCTTGCCCCCAGTTGATTTATGGTGTCTTTTAGGGTTTGTTTTTGTCGATATGTGACGCGTTTTCGTGAAATCTATTGTTGAAGTAAATAAATAAATATGGATTTTTATTAAACAAAATGTTGAATCTAATCTTACCAGATTCAAATTCGAAGACTGGCTTGTTTATGGTGATTGATGTATAACTTCATTGAATCGTCTATTTTGGATCAGCTTTTGCATATTAGTTTCTCGTTTTGAAAATAATTTCAAAATGTGATAGTTATTACTGTTACGTCGGGGCGTTTTAACGATGTCACTTTACCGAAGCAATTGTTTCTTCTTCGTTCATTTCTTAATCTTCCACTGTTTTATTTTCAGACTTTAGCTTTATTTTTTGTCGTTTATGTTATAATCACTGAAAACTATACGATATTATTCATCTACTTTGTTGCTGATTCTTCTAGTACAGAAGTTTTGGCGTGAGTGAGGTTATATCTCATCCTTTCTAAAGGAGATTAAAATTATTCAGTTTAGCGTTTCCCATAGTTCCTGTGCTTAGAAAGTTATGTATTGTTTCTTTTAAAAACTCACCAATATCAATATGGAATCCCCATCAACAGATTATTTGTTGGGCGTTGAGGTATAGTTTATTGTTGTTGAAAGAGTATAGTTCTGGCAGATTATAGCTTTTTCCTCCTTTTCGGGATAGAGAATGTCTGTTGTGGGATGTTTTGCTTGAGAGGAATAGCAGATTCTAGAGGGGGTTGAGAGGCCTTGGGAGAAAGTTTGGGATATTATTACATTTAATTCCTTCAAGTGGTGTCGGCATCAAAGATTTTTTGTAATTATCAGTTAAGAGTTGTTCTTTTGGATTGAAGATCCTTTCTATAGGCAGGTCTTCTTTTGTCAAGCTTGTTTTTTTGTATGGCATTTGTTTTTTCTTTCATTTCATCATTTCATCTTAATAAAAGTGCGAGTATAATTGTTATCTACTATGTTTAAGCCCTTGTATTTTTCTTTCTTTTTATTATTATTATAAGAAATAAGAGATTTTGTATTCACAAAAAAGAACAGCCTAGGGGGCAGGAGGTGGGAGGAATCCCTTCCAAGGGAGCAAACAAGATTTAGCTTGCAGTATTTTATATTTTTATATCAGAATATTTTTAATGAATTAGTTTGAGATTAAGTATAAGAAGGTATGAGGATAATTGATCCTCTTACTTGAATGAAACAATGAAGTATTAGATTTAGAATAAGAAAAAATCAAACCGTGCCCCTAGAAAAACTGCATCATGCCATTTCTTATGCAAAAGGAAAAAATAATCCTTTATGAAATAACTTCAAAGTTTCTACTTTTTTTTTGAACAAGAAACAAACTTTTCATTGATTTAATGAAAAGGAACAGAAAATTGTTCAATGATACAAACCTCCGAAGTAGTGAGAAAATAAACAAAACGGAAAACAGAACAACAAACTAAGCAAAAAGAACAAAAGCTAAGGGGGAGTTATAAAAACCTCCCAATTAGAACAAATCATACTCAGAGAGTAATTAACAAAAACATTGGAAAGAGCACACCATTGGGAAGCCTTAAACTTTGCTAGGAGGATCTCGACGTTTCCTCTTGAAAAGCCTCTGATTTCTCTCGAACCAAATATCAGACAACACAGCTTTGACTCCATTACACCAAAGAAGACCCGCCTTTGACGCGAGACACAGGCCAATCAACAGAGAATGAACGTTCTTTGCGACTTCGAAATCAAAAACTCAAGAAACCTCAAAGATCTCGAAGAAAAGCCTCCAGCAAGTAGTCGAAAAAGAGCACCGAAAGAACATATGTTGCTGGAACTCACAAGACTTTCCAGACAGAGAGCAAATGGAAGGAAAAAACACCATAGAAGGGTTTTTCTTTTGAAGAATATCCACTGTATTAACTTTGACAAAGAGGAGAATCCAAGTAAAGACATTTCTACTTTAAAAGATTGGAAAACTTTAATCCTATTCCTTGGGCCCTGAAGATGAATTTACTTAAACTAACGTAGACAATCCTTAAATTTGCTCCAAAAATCAGGCAACATAAATTGAGAAAGATCAATTTCATGATGCTTTTATTATCTTGTGAGCTGACCTTTTAAGCCCCTGTAATATACATTGAAACCACTTGTTTAAGTAATACAACAACTAATTCAGATCAAAATTTTTTAAGATGCTTATGCCAATGTGGAACTGCTTATCAGTTGTTTTGTATACAATCTTGCAAAATTGACTGGCCATTATGGCTGACCTTTTTGGTTATGGATGGTTTTGCAGATCCTCCTTTATTCACAACTAGTTTTACAATCAAATGTTATAGTAAATTCTCCTCTATGTACTTGTACATTACTAATTATGGAGTTTTACTTCCTATCTCTGTATGAAAGTTGACAATGTTAGAAATAGTGGCAAGAAATCTATTAACACACTGGAAATGGTTTTGTATCTGATGCTTATTAAACAAAAAATTGCAATTATATCGTTCTTTATCAATTACCGATTGATAACCACTTGGTTTTAGCTTTTAGCTTTTGAAAATTAATCTTATAAATATTAATTCCACCTAGCACTTTCTTTGTTTTGTTTTATACTTTTCAAAAATGTTTTCAAAATTTAAGTGAAAACTAAAAAGAGTAGTTTTAAAAAGCTTTAATTTTGTTTTTGTACTTCCACCTCGACCCTCTGCCTCTAGGCTGTTTTTCTGCCTTTTTTGGGCTCCTCTTCTTTTTGAATGAAATATTATTGTTTCTTATATAAAAAAAAAAGTAATTTGGCTGAAGATTTCAAAGGTTTCTTTAAGGAAGGTAAAAAATATAGTACAGAAATTGTGAGAAAAAAGCACCATTTTTTAAATTAAAAAACTACAAAATCTTGTGCATTCTGTCCAGATGATTGATTAGGTGATTGCTTTTCAATTTTGATTTGTGATTAAACACCTTATGCATGATTATTTCTTTCCACCATGGCGTGGTTTCATGATTTTATGGAAATGACTCTCTGTTAGGTTTTGCTTCTGATGCATTCACCTCAACGAAGGAGGACAGCTTAATTATGGATATTCCTGCAACTGTAGCAGCTGTTAAGAACCCTTCTTCAAAGATAGTCTATGACGACTACAATCACGAGCGATTTCCTCCGGGTGATCCTAGCAAGCGTGCATTTGCTTATTTTGTCTTGTCAGGTGGTCGGTTTGTCTATGCTTCCTTGATTCGTCTCCTTGTTCTCAAGTTTGTTCTTAGCATGTCAGCCAGTAAGGATGTTCTTGCCCTGGCCTCGCTCGAGGTTGACCTCTCAAGCATCGAGCCTGGTTCCACCGTGACTGTCAAGTGGCGTGGAAAGCCAGTTTTCATCAGGCGACGAACTGAGGACGATATTAACTTAGCAAATAGCGTGGACATTGGATCTCTTCGTGACCCACAGCAGGATGAAGAAAGAGTTAAGAATCCAGAATGGCTTATCGTGATTGGCGTCTGCACACATCTCGGTTGCATCCCCTTGCCAAATGCTGGAGACTATGGTGGGTGGTTTTGCCCCTGCCATGGTTCTCATTACGATGTTTCTGGTAGGATCCGCAAAGGGCCAGCACCCTACAATTTGGAGGTACCCACCTACACCTTTTTGGAGGACAACAAGCTTTTGATCGGTTAAGAAACCAAGCTCTTCTTTTCGAGGTAATACAGAGGTCCGAGCGACCCCCAATTTTTATTTAAAAGCAGATAAATGGCAAAGAATCTGTTTGCAGACCTGTTTTGTTTCTTTTCTCCTCAAATTCCTTTGAAATCTTTATGCAATGAGAACACTGTAACTGTCATTGCTTGGTGCATGAATGAATCTGAATAATTTGACTGAATGAGTGAAAGGTATAGATTAGATGATGAGATACCCATACTGTTGAGAAACTGGGAACATTAACATTCAATTTTGATGTCGTATCTGTATGATTCATGTGTTTGGAAATTGTTGTACAAGTTCCAAAATTGTTTGCTTTCTTTCCAGAGGAATGTTGGAGTGATTACTAATTGAGTTTGAACTGCTAGAAAAAGGTAGAGAAGTGGTAACAAGGAATGATTATTGCTCAAAAATGCCTTTGACATAGACCATGCCTTTTTCACTCAACTGAAGCTCCTTTCTCATAAGCTCTACTTGTTGGTCATCTCCTTGAACAATAATCTCTGCAAGCTTTCCACCTTCTGCAACTATCATTTTGACCTTTATCTCCTCCCTT

mRNA sequence

ATTTTGCAATAAAATGAAAAGAAAAAAAATGTTCCACGCCTTAAGCAAAAAGGGACCGGGGGTCAAAATTCTGTAAGCTTCATATACCTTCGAATTCTGTCGCCTCCTCCTCCCTCTGAATTCTCAACACACCTCGAAGCTCCGATTCCCAAAACATTTTTCACGACGGAAGCTTTCGATTTGTAGACGAAGATGTTGAGGATTGCGGCTCGAAGGCTCTCTTCACTCTCTTCTTCCCATTTTAGGCCCACTCTCGCTTCTTCCTTCTCCGGTTCTCAGAAGATCGCTGATTCCGCTGATTCCGTCCCTTCCAATGATTTCAGATCCGTCGCCTCCTTCAACACCTTCCCTTTCGGTCTTCACTTTGATCGTAATTGCAGAGGTTTTGCTTCTGATGCATTCACCTCAACGAAGGAGGACAGCTTAATTATGGATATTCCTGCAACTGTAGCAGCTGTTAAGAACCCTTCTTCAAAGATAGTCTATGACGACTACAATCACGAGCGATTTCCTCCGGGTGATCCTAGCAAGCGTGCATTTGCTTATTTTGTCTTGTCAGGTGGTCGGTTTGTCTATGCTTCCTTGATTCGTCTCCTTGTTCTCAAGTTTGTTCTTAGCATGTCAGCCAGTAAGGATGTTCTTGCCCTGGCCTCGCTCGAGGTTGACCTCTCAAGCATCGAGCCTGGTTCCACCGTGACTGTCAAGTGGCGTGGAAAGCCAGTTTTCATCAGGCGACGAACTGAGGACGATATTAACTTAGCAAATAGCGTGGACATTGGATCTCTTCGTGACCCACAGCAGGATGAAGAAAGAGTTAAGAATCCAGAATGGCTTATCGTGATTGGCGTCTGCACACATCTCGGTTGCATCCCCTTGCCAAATGCTGGAGACTATGGTGGGTGGTTTTGCCCCTGCCATGGTTCTCATTACGATGTTTCTGGTAGGATCCGCAAAGGGCCAGCACCCTACAATTTGGAGGTACCCACCTACACCTTTTTGGAGGACAACAAGCTTTTGATCGGTTAAGAAACCAAGCTCTTCTTTTCGAGGTAATACAGAGGTCCGAGCGACCCCCAATTTTTATTTAAAAGCAGATAAATGGCAAAGAATCTGTTTGCAGACCTGTTTTGTTTCTTTTCTCCTCAAATTCCTTTGAAATCTTTATGCAATGAGAACACTGTAACTGTCATTGCTTGGTGCATGAATGAATCTGAATAATTTGACTGAATGAGTGAAAGGTATAGATTAGATGATGAGATACCCATACTGTTGAGAAACTGGGAACATTAACATTCAATTTTGATGTCGTATCTGTATGATTCATGTGTTTGGAAATTGTTGTACAAGTTCCAAAATTGTTTGCTTTCTTTCCAGAGGAATGTTGGAGTGATTACTAATTGAGTTTGAACTGCTAGAAAAAGGTAGAGAAGTGGTAACAAGGAATGATTATTGCTCAAAAATGCCTTTGACATAGACCATGCCTTTTTCACTCAACTGAAGCTCCTTTCTCATAAGCTCTACTTGTTGGTCATCTCCTTGAACAATAATCTCTGCAAGCTTTCCACCTTCTGCAACTATCATTTTGACCTTTATCTCCTCCCTT

Coding sequence (CDS)

ATGTTGAGGATTGCGGCTCGAAGGCTCTCTTCACTCTCTTCTTCCCATTTTAGGCCCACTCTCGCTTCTTCCTTCTCCGGTTCTCAGAAGATCGCTGATTCCGCTGATTCCGTCCCTTCCAATGATTTCAGATCCGTCGCCTCCTTCAACACCTTCCCTTTCGGTCTTCACTTTGATCGTAATTGCAGAGGTTTTGCTTCTGATGCATTCACCTCAACGAAGGAGGACAGCTTAATTATGGATATTCCTGCAACTGTAGCAGCTGTTAAGAACCCTTCTTCAAAGATAGTCTATGACGACTACAATCACGAGCGATTTCCTCCGGGTGATCCTAGCAAGCGTGCATTTGCTTATTTTGTCTTGTCAGGTGGTCGGTTTGTCTATGCTTCCTTGATTCGTCTCCTTGTTCTCAAGTTTGTTCTTAGCATGTCAGCCAGTAAGGATGTTCTTGCCCTGGCCTCGCTCGAGGTTGACCTCTCAAGCATCGAGCCTGGTTCCACCGTGACTGTCAAGTGGCGTGGAAAGCCAGTTTTCATCAGGCGACGAACTGAGGACGATATTAACTTAGCAAATAGCGTGGACATTGGATCTCTTCGTGACCCACAGCAGGATGAAGAAAGAGTTAAGAATCCAGAATGGCTTATCGTGATTGGCGTCTGCACACATCTCGGTTGCATCCCCTTGCCAAATGCTGGAGACTATGGTGGGTGGTTTTGCCCCTGCCATGGTTCTCATTACGATGTTTCTGGTAGGATCCGCAAAGGGCCAGCACCCTACAATTTGGAGGTACCCACCTACACCTTTTTGGAGGACAACAAGCTTTTGATCGGTTAA

Protein sequence

MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDRNCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFVLSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIRRRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCPCHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG
Homology
BLAST of MC04g1120 vs. ExPASy Swiss-Prot
Match: P51132 (Cytochrome b-c1 complex subunit Rieske-2, mitochondrial OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 2.6e-112
Identity = 204/280 (72.86%), Postives = 235/280 (83.93%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFR---PTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLH 60
           MLRIA RR SSLS    R   P+ ++  S +   +D   S P +   S+AS         
Sbjct: 1   MLRIAGRRASSLSRWPVRSVAPSSSAFISANHFSSDDDSSSPRSISPSLASV-------- 60

Query: 61  FDRNCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFA 120
           F  + RGF+S++ +   +  L+ D+P TVAA+KNP+SKIVYD++NHER+PPGDPSKRAFA
Sbjct: 61  FLHHTRGFSSNSVSHAHDMGLVPDLPPTVAAIKNPTSKIVYDEHNHERYPPGDPSKRAFA 120

Query: 121 YFVLSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPV 180
           YFVL+GGRFVYASL+RLL+LKFVLSMSASKDVLALASLEVDLSSIEPG+TVTVKWRGKPV
Sbjct: 121 YFVLTGGRFVYASLVRLLILKFVLSMSASKDVLALASLEVDLSSIEPGTTVTVKWRGKPV 180

Query: 181 FIRRRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGW 240
           FIRRRTEDDINLANSVD+GSLRDPQQD ERVK+PEWL+VIGVCTHLGCIPLPNAGD+GGW
Sbjct: 181 FIRRRTEDDINLANSVDLGSLRDPQQDAERVKSPEWLVVIGVCTHLGCIPLPNAGDFGGW 240

Query: 241 FCPCHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 278
           FCPCHGSHYD+SGRIRKGPAPYNLEVPTY+FLE+NKLLIG
Sbjct: 241 FCPCHGSHYDISGRIRKGPAPYNLEVPTYSFLEENKLLIG 272

BLAST of MC04g1120 vs. ExPASy Swiss-Prot
Match: P51133 (Cytochrome b-c1 complex subunit Rieske-3, mitochondrial OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 5.0e-111
Identity = 206/281 (73.31%), Postives = 232/281 (82.56%), Query Frame = 0

Query: 1   MLRIAARRLSS----LSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGL 60
           MLRIA R+LSS     SSS F      +F+      DS+    S    S+AS        
Sbjct: 1   MLRIAGRKLSSSAATRSSSAFFTRNPFTFTD-----DSSSPARSPSPASLAS-------- 60

Query: 61  HFDRNCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAF 120
            F    RGF+S++ +   +  L+ D+PATVAA+KNPSSKIVYDD NHER+PPGDPSKRAF
Sbjct: 61  QFLDQFRGFSSNSVSPAHQTGLVSDLPATVAAIKNPSSKIVYDDSNHERYPPGDPSKRAF 120

Query: 121 AYFVLSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKP 180
           AYFVL+GGRFVYASL+RLL+LKFVLSMSASKDVLALASLEVDLSSIEPG+TVTVKWRGKP
Sbjct: 121 AYFVLTGGRFVYASLVRLLILKFVLSMSASKDVLALASLEVDLSSIEPGTTVTVKWRGKP 180

Query: 181 VFIRRRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGG 240
           VFIRRRTE+DINLANSVD+GSLRDPQQD ERVKNPEWL+VIGVCTHLGCIPLPNAGD+GG
Sbjct: 181 VFIRRRTEEDINLANSVDLGSLRDPQQDAERVKNPEWLVVIGVCTHLGCIPLPNAGDFGG 240

Query: 241 WFCPCHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 278
           WFCPCHGSHYD+SGRIRKGPAPYNLEVPTY+F+E+NKLLIG
Sbjct: 241 WFCPCHGSHYDISGRIRKGPAPYNLEVPTYSFMEENKLLIG 268

BLAST of MC04g1120 vs. ExPASy Swiss-Prot
Match: P51135 (Cytochrome b-c1 complex subunit Rieske-5, mitochondrial OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.1e-110
Identity = 205/281 (72.95%), Postives = 232/281 (82.56%), Query Frame = 0

Query: 1   MLRIAARRLSS----LSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGL 60
           MLRIA R+LSS     SSS F      +F+      DS+    S    S+AS        
Sbjct: 1   MLRIAGRKLSSSAAARSSSAFFTRNPFTFTD-----DSSSPTRSPSPTSLAS-------- 60

Query: 61  HFDRNCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAF 120
            F    RGF+S++ +   +  L+ D+PATVAA+KNPSSKIVYDD NHER+PPGDPSKRAF
Sbjct: 61  QFLDQFRGFSSNSVSPAHQTGLVSDLPATVAAIKNPSSKIVYDDSNHERYPPGDPSKRAF 120

Query: 121 AYFVLSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKP 180
           AYFVL+GGRFVYASL+RLL+LKFVLSMSASKDVLALASLEVDLSSIEPG+TVTVKWRGKP
Sbjct: 121 AYFVLTGGRFVYASLVRLLILKFVLSMSASKDVLALASLEVDLSSIEPGTTVTVKWRGKP 180

Query: 181 VFIRRRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGG 240
           VFIRRRT++DINLANSVD+GSLRDPQQD ERVKNPEWL+VIGVCTHLGCIPLPNAGD+GG
Sbjct: 181 VFIRRRTDEDINLANSVDLGSLRDPQQDAERVKNPEWLVVIGVCTHLGCIPLPNAGDFGG 240

Query: 241 WFCPCHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 278
           WFCPCHGSHYD+SGRIRKGPAPYNLEVPTY+F+E+NKLLIG
Sbjct: 241 WFCPCHGSHYDISGRIRKGPAPYNLEVPTYSFMEENKLLIG 268

BLAST of MC04g1120 vs. ExPASy Swiss-Prot
Match: Q9LYR2 (Cytochrome b-c1 complex subunit Rieske-2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=UCR1-2 PE=1 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 2.5e-110
Identity = 203/279 (72.76%), Postives = 229/279 (82.08%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSF--SGSQKIADSADSVPSNDFRSVASFNTFPFGLHF 60
           MLR+A RRL S+S    R + A+SF  S    ++D  +S  S+  RSV S +   F  + 
Sbjct: 1   MLRVAGRRLFSVSQ---RSSTATSFVLSRDHTLSDGGNS--SSASRSVPSADLSSFNSYH 60

Query: 61  DRNCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAY 120
               RGFAS   T   E     ++PATV AVK P+SKIVYDD+NHER+PPGDPSKRAFAY
Sbjct: 61  RSVIRGFASQVITQGNEIGFGSEVPATVEAVKTPNSKIVYDDHNHERYPPGDPSKRAFAY 120

Query: 121 FVLSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVF 180
           FVLSGGRFVYAS++RLLVLK ++SMSASKDVLALASLEVDL SIEPG+TVTVKWRGKPVF
Sbjct: 121 FVLSGGRFVYASVLRLLVLKLIVSMSASKDVLALASLEVDLGSIEPGTTVTVKWRGKPVF 180

Query: 181 IRRRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWF 240
           IRRRTEDDI LANSVD+GSLRDPQ+D  RVKNPEWLIV+GVCTHLGCIPLPNAGDYGGWF
Sbjct: 181 IRRRTEDDIKLANSVDVGSLRDPQEDSVRVKNPEWLIVVGVCTHLGCIPLPNAGDYGGWF 240

Query: 241 CPCHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 278
           CPCHGSHYD+SGRIRKGPAPYNLEVPTY+FLE+NKLLIG
Sbjct: 241 CPCHGSHYDISGRIRKGPAPYNLEVPTYSFLEENKLLIG 274

BLAST of MC04g1120 vs. ExPASy Swiss-Prot
Match: P37841 (Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Solanum tuberosum OX=4113 GN=FES1 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 7.2e-110
Identity = 202/277 (72.92%), Postives = 232/277 (83.75%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLR+A RRLSS ++       +S+F        + DS P+   RS +   T  F    D+
Sbjct: 1   MLRVAGRRLSSSAARS-----SSTFFTRSSFTVTDDSSPA---RSPSPSLTSSF---LDQ 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
             RGF+S++ +   +  L+ D+PATVAA+KNPSSKIVYDD NHER+PPGDPSKRAFAYFV
Sbjct: 61  -IRGFSSNSVSPAHQLGLVSDLPATVAAIKNPSSKIVYDDSNHERYPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           L+GGRFVYAS +RLL+LKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR
Sbjct: 121 LTGGRFVYASSVRLLILKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRT+DDI LANSVD+G+LRDPQQD ERVKNPEWL+V+GVCTHLGCIPLPNAGD+GGWFCP
Sbjct: 181 RRTDDDIKLANSVDLGTLRDPQQDAERVKNPEWLVVVGVCTHLGCIPLPNAGDFGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 278
           CHGSHYD+SGRIRKGPAPYNLEVPTY+FLE+NKLLIG
Sbjct: 241 CHGSHYDISGRIRKGPAPYNLEVPTYSFLEENKLLIG 265

BLAST of MC04g1120 vs. NCBI nr
Match: XP_022141474.1 (cytochrome b-c1 complex subunit Rieske-4, mitochondrial-like [Momordica charantia])

HSP 1 Score: 554 bits (1427), Expect = 7.15e-199
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR
Sbjct: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
           NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV
Sbjct: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR
Sbjct: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP
Sbjct: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277
           CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG
Sbjct: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277

BLAST of MC04g1120 vs. NCBI nr
Match: XP_022938899.1 (cytochrome b-c1 complex subunit Rieske-4, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 511 bits (1316), Expect = 5.96e-182
Identity = 251/277 (90.61%), Postives = 262/277 (94.58%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLRIAARRLSSLSSSHFRPT+ASS  GS KI D  DS PSNDFRSV SFNTFPFG  FDR
Sbjct: 1   MLRIAARRLSSLSSSHFRPTIASSLPGSHKIIDGGDSAPSNDFRSVTSFNTFPFGPFFDR 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
           NCRGFASDA TSTKE  L+ D+PATVAAVKNP+SKIVYD+YNHERFPPGDPSKRAFAYFV
Sbjct: 61  NCRGFASDALTSTKETDLVPDVPATVAAVKNPTSKIVYDEYNHERFPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPG+TVTVKWRGKPVFIR
Sbjct: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGATVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRTEDDI LANSVDIGSLRDPQQDEERVK+PEWLIV+GVCTHLGCIPLPNAGDYGGWFCP
Sbjct: 181 RRTEDDIKLANSVDIGSLRDPQQDEERVKDPEWLIVVGVCTHLGCIPLPNAGDYGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277
           CHGSHYD+SGRIRKGPAPYNLEVPTYTFL++NKLLIG
Sbjct: 241 CHGSHYDISGRIRKGPAPYNLEVPTYTFLDENKLLIG 277

BLAST of MC04g1120 vs. NCBI nr
Match: XP_023550035.1 (cytochrome b-c1 complex subunit Rieske-4, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508 bits (1309), Expect = 6.95e-181
Identity = 250/277 (90.25%), Postives = 261/277 (94.22%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLRIAARRLSSLSSSHFRPT+ASS  GS KI D  DS PSNDFRSV SFNTFPFG  FDR
Sbjct: 1   MLRIAARRLSSLSSSHFRPTIASSLPGSHKIIDGGDSAPSNDFRSVTSFNTFPFGPFFDR 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
           NCRGFASDA TSTKE  L+ D+PATVAAVKNP+SKIVYD+YNHERFPPGDPSKRAFAYFV
Sbjct: 61  NCRGFASDALTSTKETDLVPDVPATVAAVKNPTSKIVYDEYNHERFPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPG+TVTVKWRGKPVFIR
Sbjct: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGATVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRTEDDI LANSVDIGSLRDPQQD ERVK+PEWLIV+GVCTHLGCIPLPNAGDYGGWFCP
Sbjct: 181 RRTEDDIKLANSVDIGSLRDPQQDGERVKDPEWLIVVGVCTHLGCIPLPNAGDYGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277
           CHGSHYD+SGRIRKGPAPYNLEVPTYTFL++NKLLIG
Sbjct: 241 CHGSHYDISGRIRKGPAPYNLEVPTYTFLDENKLLIG 277

BLAST of MC04g1120 vs. NCBI nr
Match: KAG6579100.1 (hypothetical protein SDJN03_23548, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 503 bits (1296), Expect = 6.65e-179
Identity = 248/277 (89.53%), Postives = 259/277 (93.50%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLRIAARRLSSLSSSHFRPT+ASS  GS K  D  DS PSND RSV SFNTFPFG  FDR
Sbjct: 1   MLRIAARRLSSLSSSHFRPTIASSLPGSHKTIDGGDSAPSNDSRSVTSFNTFPFGPFFDR 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
           NCRGFASDA TSTKE  L+ D+PATVAAVKNP+SKIVYD+YNHERFPPGDPSKRAFAYFV
Sbjct: 61  NCRGFASDALTSTKETDLVPDVPATVAAVKNPTSKIVYDEYNHERFPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPG+TVTVKWRGKPVFIR
Sbjct: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGATVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRTEDDI LANSVDIGSLRDPQQD ERVK+PEWLIV+GVCTHLGCIPLPNAGDYGGWFCP
Sbjct: 181 RRTEDDIKLANSVDIGSLRDPQQDGERVKDPEWLIVVGVCTHLGCIPLPNAGDYGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277
           CHGSHYD+SGRIRKGPAPYNLEVPTYTFL++NKLLIG
Sbjct: 241 CHGSHYDISGRIRKGPAPYNLEVPTYTFLDENKLLIG 277

BLAST of MC04g1120 vs. NCBI nr
Match: XP_023550036.1 (cytochrome b-c1 complex subunit Rieske-4, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503 bits (1294), Expect = 1.20e-178
Identity = 249/277 (89.89%), Postives = 260/277 (93.86%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLRIAARR SSLSSSHFRPT++SSF GS KI DS DS   NDFRS   FNTFP G HF R
Sbjct: 1   MLRIAARRFSSLSSSHFRPTISSSFPGSHKIVDSGDS---NDFRSGGFFNTFPSGPHFGR 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
           NCRGFASDA TSTK+D LI+DIPATVAA+KNPSSKIVYD+YNHERFPPGDPSKRAFAYFV
Sbjct: 61  NCRGFASDALTSTKDDGLILDIPATVAAIKNPSSKIVYDEYNHERFPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR
Sbjct: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRTEDDI +ANSVDIGSLRDPQQDEERVKNPEWLIV+GVCTHLGCIPLPNAGDYGGWFCP
Sbjct: 181 RRTEDDIKVANSVDIGSLRDPQQDEERVKNPEWLIVVGVCTHLGCIPLPNAGDYGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277
           CHGSHYD+SGRIRKGPAPYNLEVPTYTFL++NKLLIG
Sbjct: 241 CHGSHYDISGRIRKGPAPYNLEVPTYTFLDENKLLIG 274

BLAST of MC04g1120 vs. ExPASy TrEMBL
Match: A0A6J1CIR6 (Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011857 PE=3 SV=1)

HSP 1 Score: 554 bits (1427), Expect = 3.46e-199
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR
Sbjct: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
           NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV
Sbjct: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR
Sbjct: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP
Sbjct: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277
           CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG
Sbjct: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277

BLAST of MC04g1120 vs. ExPASy TrEMBL
Match: A0A6J1FFG4 (Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111444974 PE=3 SV=1)

HSP 1 Score: 511 bits (1316), Expect = 2.88e-182
Identity = 251/277 (90.61%), Postives = 262/277 (94.58%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLRIAARRLSSLSSSHFRPT+ASS  GS KI D  DS PSNDFRSV SFNTFPFG  FDR
Sbjct: 1   MLRIAARRLSSLSSSHFRPTIASSLPGSHKIIDGGDSAPSNDFRSVTSFNTFPFGPFFDR 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
           NCRGFASDA TSTKE  L+ D+PATVAAVKNP+SKIVYD+YNHERFPPGDPSKRAFAYFV
Sbjct: 61  NCRGFASDALTSTKETDLVPDVPATVAAVKNPTSKIVYDEYNHERFPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPG+TVTVKWRGKPVFIR
Sbjct: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGATVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRTEDDI LANSVDIGSLRDPQQDEERVK+PEWLIV+GVCTHLGCIPLPNAGDYGGWFCP
Sbjct: 181 RRTEDDIKLANSVDIGSLRDPQQDEERVKDPEWLIVVGVCTHLGCIPLPNAGDYGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277
           CHGSHYD+SGRIRKGPAPYNLEVPTYTFL++NKLLIG
Sbjct: 241 CHGSHYDISGRIRKGPAPYNLEVPTYTFLDENKLLIG 277

BLAST of MC04g1120 vs. ExPASy TrEMBL
Match: A0A6J1JWJ5 (Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488984 PE=3 SV=1)

HSP 1 Score: 500 bits (1288), Expect = 4.78e-178
Identity = 249/277 (89.89%), Postives = 259/277 (93.50%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLRIAARR SSLSSSHFRPT+ASS  GS KI DS DS   NDFRS   FNTFP G HFDR
Sbjct: 1   MLRIAARRFSSLSSSHFRPTIASSLPGSHKIVDSGDS---NDFRSGGFFNTFPSGPHFDR 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
           +CRGFASDA TSTK+D LI+DIPATVAA+KNPSSKIVYD+YNHERFPPGDPSKRAFAYFV
Sbjct: 61  SCRGFASDALTSTKDDGLILDIPATVAAIKNPSSKIVYDEYNHERFPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR
Sbjct: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRTEDDI LANSVDI SLRDPQQDEERVKNPEWLIV+GVCTHLGCIPLPNAGDYGGWFCP
Sbjct: 181 RRTEDDIKLANSVDIESLRDPQQDEERVKNPEWLIVVGVCTHLGCIPLPNAGDYGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277
           CHGSHYD+SGRIRKGPAPYNLEVPTYTFL++NKLLIG
Sbjct: 241 CHGSHYDISGRIRKGPAPYNLEVPTYTFLDENKLLIG 274

BLAST of MC04g1120 vs. ExPASy TrEMBL
Match: A0A6J1FL42 (Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111444975 PE=3 SV=1)

HSP 1 Score: 499 bits (1286), Expect = 9.65e-178
Identity = 249/277 (89.89%), Postives = 259/277 (93.50%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLRIAARR SSLSSSHFRPT+ASS  GS KIADS DS   NDFRS   FNTFP G HFDR
Sbjct: 1   MLRIAARRFSSLSSSHFRPTIASSLPGSHKIADSGDS---NDFRSGGFFNTFPSGPHFDR 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
           +CRGFASDA TSTK+D LI+DIPATVAA+KNPSSKIVYD+YNHERFPPGDPSKRAFAYFV
Sbjct: 61  SCRGFASDALTSTKDDGLILDIPATVAAIKNPSSKIVYDEYNHERFPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR
Sbjct: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRTEDDI  ANSVDI SLRDPQQDEERVKNPEWLIV+GVCTHLGCIPLPNAGDYGGWFCP
Sbjct: 181 RRTEDDIKSANSVDIESLRDPQQDEERVKNPEWLIVVGVCTHLGCIPLPNAGDYGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277
           CHGSHYD+SGRIRKGPAPYNLEVPTYTFL++NKLLIG
Sbjct: 241 CHGSHYDISGRIRKGPAPYNLEVPTYTFLDENKLLIG 274

BLAST of MC04g1120 vs. ExPASy TrEMBL
Match: A0A1S3CPE6 (Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103503117 PE=3 SV=1)

HSP 1 Score: 499 bits (1284), Expect = 1.95e-177
Identity = 251/277 (90.61%), Postives = 257/277 (92.78%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSFSGSQKIADSADSVPSNDFRSVASFNTFPFGLHFDR 60
           MLRIAARRLSSLSSSHFRPT+ASS  GS  I DSADS   NDFRS   FNTFP   HFDR
Sbjct: 1   MLRIAARRLSSLSSSHFRPTIASSLPGSHNIIDSADS---NDFRSAGFFNTFPIRPHFDR 60

Query: 61  NCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120
           N RGFASDA  STK+D LIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV
Sbjct: 61  NSRGFASDALASTKDDGLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAYFV 120

Query: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180
           LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR
Sbjct: 121 LSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVFIR 180

Query: 181 RRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWFCP 240
           RRTEDDI LANSVDIGSLRDPQQDEERVK+PEWL+V+GVCTHLGCIPLPNAGDYGGWFCP
Sbjct: 181 RRTEDDIKLANSVDIGSLRDPQQDEERVKDPEWLVVVGVCTHLGCIPLPNAGDYGGWFCP 240

Query: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 277
           CHGSHYDVSGRIRKGPAPYNLEVPTY+FLE NKLLIG
Sbjct: 241 CHGSHYDVSGRIRKGPAPYNLEVPTYSFLEGNKLLIG 274

BLAST of MC04g1120 vs. TAIR 10
Match: AT5G13440.1 (Ubiquinol-cytochrome C reductase iron-sulfur subunit )

HSP 1 Score: 399.8 bits (1026), Expect = 1.8e-111
Identity = 203/279 (72.76%), Postives = 229/279 (82.08%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSF--SGSQKIADSADSVPSNDFRSVASFNTFPFGLHF 60
           MLR+A RRL S+S    R + A+SF  S    ++D  +S  S+  RSV S +   F  + 
Sbjct: 1   MLRVAGRRLFSVSQ---RSSTATSFVLSRDHTLSDGGNS--SSASRSVPSADLSSFNSYH 60

Query: 61  DRNCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDPSKRAFAY 120
               RGFAS   T   E     ++PATV AVK P+SKIVYDD+NHER+PPGDPSKRAFAY
Sbjct: 61  RSVIRGFASQVITQGNEIGFGSEVPATVEAVKTPNSKIVYDDHNHERYPPGDPSKRAFAY 120

Query: 121 FVLSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVKWRGKPVF 180
           FVLSGGRFVYAS++RLLVLK ++SMSASKDVLALASLEVDL SIEPG+TVTVKWRGKPVF
Sbjct: 121 FVLSGGRFVYASVLRLLVLKLIVSMSASKDVLALASLEVDLGSIEPGTTVTVKWRGKPVF 180

Query: 181 IRRRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNAGDYGGWF 240
           IRRRTEDDI LANSVD+GSLRDPQ+D  RVKNPEWLIV+GVCTHLGCIPLPNAGDYGGWF
Sbjct: 181 IRRRTEDDIKLANSVDVGSLRDPQEDSVRVKNPEWLIVVGVCTHLGCIPLPNAGDYGGWF 240

Query: 241 CPCHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 278
           CPCHGSHYD+SGRIRKGPAPYNLEVPTY+FLE+NKLLIG
Sbjct: 241 CPCHGSHYDISGRIRKGPAPYNLEVPTYSFLEENKLLIG 274

BLAST of MC04g1120 vs. TAIR 10
Match: AT5G13430.1 (Ubiquinol-cytochrome C reductase iron-sulfur subunit )

HSP 1 Score: 387.5 bits (994), Expect = 9.0e-108
Identity = 197/286 (68.88%), Postives = 223/286 (77.97%), Query Frame = 0

Query: 1   MLRIAARRLSSLSSSHFRPTLASSF---------SGSQKIADSADSVPSNDFRSVASFNT 60
           MLR+A RRL S+S    R + A+SF          G    + +  S+PS D  S      
Sbjct: 1   MLRVAGRRLFSVSQ---RSSTATSFVVSRDHTLSDGGGDSSSAPRSLPSADLSS------ 60

Query: 61  FPFGLHFDRNCRGFASDAFTSTKEDSLIMDIPATVAAVKNPSSKIVYDDYNHERFPPGDP 120
                +     RGF+S       E     ++PATV AVK P+SKIVYDD+NHER+PPGDP
Sbjct: 61  -----YHRSLIRGFSSQVLAQGNEIGFGSEVPATVEAVKTPNSKIVYDDHNHERYPPGDP 120

Query: 121 SKRAFAYFVLSGGRFVYASLIRLLVLKFVLSMSASKDVLALASLEVDLSSIEPGSTVTVK 180
           SKRAFAYFVLSGGRFVYAS++RLLVLK ++SMSASKDVLALASLEVDL SIEPG+TVTVK
Sbjct: 121 SKRAFAYFVLSGGRFVYASVLRLLVLKLIVSMSASKDVLALASLEVDLGSIEPGTTVTVK 180

Query: 181 WRGKPVFIRRRTEDDINLANSVDIGSLRDPQQDEERVKNPEWLIVIGVCTHLGCIPLPNA 240
           WRGKPVFIRRRTEDDI LANSVD+GSLRDPQ+D  RVKNPEWL+V+GVCTHLGCIPLPNA
Sbjct: 181 WRGKPVFIRRRTEDDIKLANSVDVGSLRDPQEDSVRVKNPEWLVVVGVCTHLGCIPLPNA 240

Query: 241 GDYGGWFCPCHGSHYDVSGRIRKGPAPYNLEVPTYTFLEDNKLLIG 278
           GDYGGWFCPCHGSHYD+SGRIRKGPAPYNLEVPTY+FLE+NKLLIG
Sbjct: 241 GDYGGWFCPCHGSHYDISGRIRKGPAPYNLEVPTYSFLEENKLLIG 272

BLAST of MC04g1120 vs. TAIR 10
Match: AT4G03280.1 (photosynthetic electron transfer C )

HSP 1 Score: 59.3 bits (142), Expect = 5.6e-09
Identity = 29/71 (40.85%), Postives = 40/71 (56.34%), Query Frame = 0

Query: 208 VKNPEWLIVIG---VCTHLGCIPLPNAGDYGGWFCPCHGSHYDVSGRIRKGPAPYNLEVP 267
           V+N + L   G   VCTHLGC+   N  +   + CPCHGS Y+  GR+ +GPAP +L + 
Sbjct: 142 VENDKTLATYGINAVCTHLGCVVPWNKAE-NKFLCPCHGSQYNAQGRVVRGPAPLSLALA 201

Query: 268 TYTFLEDNKLL 276
                E  K+L
Sbjct: 202 HADIDEAGKVL 211

BLAST of MC04g1120 vs. TAIR 10
Match: AT4G03280.2 (photosynthetic electron transfer C )

HSP 1 Score: 59.3 bits (142), Expect = 5.6e-09
Identity = 29/71 (40.85%), Postives = 40/71 (56.34%), Query Frame = 0

Query: 208 VKNPEWLIVIG---VCTHLGCIPLPNAGDYGGWFCPCHGSHYDVSGRIRKGPAPYNLEVP 267
           V+N + L   G   VCTHLGC+   N  +   + CPCHGS Y+  GR+ +GPAP +L + 
Sbjct: 123 VENDKTLATYGINAVCTHLGCVVPWNKAE-NKFLCPCHGSQYNAQGRVVRGPAPLSLALA 182

Query: 268 TYTFLEDNKLL 276
                E  K+L
Sbjct: 183 HADIDEAGKVL 192

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P511322.6e-11272.86Cytochrome b-c1 complex subunit Rieske-2, mitochondrial OS=Nicotiana tabacum OX=... [more]
P511335.0e-11173.31Cytochrome b-c1 complex subunit Rieske-3, mitochondrial OS=Nicotiana tabacum OX=... [more]
P511351.1e-11072.95Cytochrome b-c1 complex subunit Rieske-5, mitochondrial OS=Nicotiana tabacum OX=... [more]
Q9LYR22.5e-11072.76Cytochrome b-c1 complex subunit Rieske-2, mitochondrial OS=Arabidopsis thaliana ... [more]
P378417.2e-11072.92Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Solanum tuberosum OX=41... [more]
Match NameE-valueIdentityDescription
XP_022141474.17.15e-199100.00cytochrome b-c1 complex subunit Rieske-4, mitochondrial-like [Momordica charanti... [more]
XP_022938899.15.96e-18290.61cytochrome b-c1 complex subunit Rieske-4, mitochondrial-like [Cucurbita moschata... [more]
XP_023550035.16.95e-18190.25cytochrome b-c1 complex subunit Rieske-4, mitochondrial-like [Cucurbita pepo sub... [more]
KAG6579100.16.65e-17989.53hypothetical protein SDJN03_23548, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023550036.11.20e-17889.89cytochrome b-c1 complex subunit Rieske-4, mitochondrial-like [Cucurbita pepo sub... [more]
Match NameE-valueIdentityDescription
A0A6J1CIR63.46e-199100.00Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Momordica charantia OX=... [more]
A0A6J1FFG42.88e-18290.61Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Cucurbita moschata OX=3... [more]
A0A6J1JWJ54.78e-17889.89Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Cucurbita maxima OX=366... [more]
A0A6J1FL429.65e-17889.89Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Cucurbita moschata OX=3... [more]
A0A1S3CPE61.95e-17790.61Cytochrome b-c1 complex subunit Rieske, mitochondrial OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT5G13440.11.8e-11172.76Ubiquinol-cytochrome C reductase iron-sulfur subunit [more]
AT5G13430.19.0e-10868.88Ubiquinol-cytochrome C reductase iron-sulfur subunit [more]
AT4G03280.15.6e-0940.85photosynthetic electron transfer C [more]
AT4G03280.25.6e-0940.85photosynthetic electron transfer C [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005805Rieske iron-sulphur protein, C-terminalPRINTSPR00162RIESKEcoord: 249..261
score: 69.81
coord: 237..249
score: 71.54
coord: 215..226
score: 67.29
IPR004192Cytochrome b-c1 complex subunit Rieske, transmembrane domainPFAMPF02921UCR_TMcoord: 102..148
e-value: 2.7E-11
score: 43.8
IPR017941Rieske [2Fe-2S] iron-sulphur domainPFAMPF00355Rieskecoord: 160..263
e-value: 2.6E-11
score: 43.3
IPR017941Rieske [2Fe-2S] iron-sulphur domainPROSITEPS51296RIESKEcoord: 207..275
score: 17.998817
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilyGENE3D2.102.10.10coord: 146..277
e-value: 1.4E-59
score: 202.0
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilySUPERFAMILY50022ISP domaincoord: 139..276
IPR006317Ubiquinol-cytochrome c reductase, iron-sulphur subunitTIGRFAMTIGR01416TIGR01416coord: 133..277
e-value: 1.6E-55
score: 185.8
IPR014349Rieske iron-sulphur proteinPANTHERPTHR10134CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIALcoord: 1..277
NoneNo IPR availablePANTHERPTHR10134:SF36CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIALcoord: 1..277
NoneNo IPR availableCDDcd03470Rieske_cytochrome_bc1coord: 154..277
e-value: 1.40639E-86
score: 252.095
NoneNo IPR availableSUPERFAMILY81502ISP transmembrane anchorcoord: 88..150

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g1120.1MC04g1120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c
biological_process GO:1902600 proton transmembrane transport
cellular_component GO:0005750 mitochondrial respiratory chain complex III
cellular_component GO:0016020 membrane
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008121 ubiquinol-cytochrome-c reductase activity