MC04g1033 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g1033
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAAI domain-containing protein
LocationMC04: 18422219 .. 18422584 (-)
RNA-Seq ExpressionMC04g1033
SyntenyMC04g1033
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCTTCAGCACTACTTCTAAGGCTTTGATCGTGGCTGCCGGCCTTCTGATTCTGGTGGCTTCGGAGGGTTTCATTCACCGAGCCGGGGCGGCCGGAGAGTGCGGGAAGATGACGGTCGGGTTGGCCGCGGTGAGCCTGACGCCGTGCTTGGGGGCAGTCAAGGATGTGAGAGCTAAGGTTACGGCGGCGTGCTGCAGCAAGGTGGCGGGGATGTTTAATACTACTCCCAAATGCCTGTGCGCCATTCTTCTTTCGCCTATGGCTAAGCAAGCCGGAATCAACCCGGCCGTTGCCATTACCATCCCCAAACGTTGCAATATCAAAAACCGTCCCGCCGGCAAGAAGTGTGGAAGTAAGCTC

mRNA sequence

ATGGCTTCCTTCAGCACTACTTCTAAGGCTTTGATCGTGGCTGCCGGCCTTCTGATTCTGGTGGCTTCGGAGGGTTTCATTCACCGAGCCGGGGCGGCCGGAGAGTGCGGGAAGATGACGGTCGGGTTGGCCGCGGTGAGCCTGACGCCGTGCTTGGGGGCAGTCAAGGATGTGAGAGCTAAGGTTACGGCGGCGTGCTGCAGCAAGGTGGCGGGGATGTTTAATACTACTCCCAAATGCCTGTGCGCCATTCTTCTTTCGCCTATGGCTAAGCAAGCCGGAATCAACCCGGCCGTTGCCATTACCATCCCCAAACGTTGCAATATCAAAAACCGTCCCGCCGGCAAGAAGTGTGGAAGTAAGCTC

Coding sequence (CDS)

ATGGCTTCCTTCAGCACTACTTCTAAGGCTTTGATCGTGGCTGCCGGCCTTCTGATTCTGGTGGCTTCGGAGGGTTTCATTCACCGAGCCGGGGCGGCCGGAGAGTGCGGGAAGATGACGGTCGGGTTGGCCGCGGTGAGCCTGACGCCGTGCTTGGGGGCAGTCAAGGATGTGAGAGCTAAGGTTACGGCGGCGTGCTGCAGCAAGGTGGCGGGGATGTTTAATACTACTCCCAAATGCCTGTGCGCCATTCTTCTTTCGCCTATGGCTAAGCAAGCCGGAATCAACCCGGCCGTTGCCATTACCATCCCCAAACGTTGCAATATCAAAAACCGTCCCGCCGGCAAGAAGTGTGGAAGTAAGCTC

Protein sequence

MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRAKVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCGSKL
Homology
BLAST of MC04g1033 vs. NCBI nr
Match: XP_022146313.1 (non-specific lipid transfer protein GPI-anchored 2-like [Momordica charantia])

HSP 1 Score: 230 bits (587), Expect = 6.88e-76
Identity = 119/119 (100.00%), Postives = 119/119 (100.00%), Query Frame = 0

Query: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60
           MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA
Sbjct: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60

Query: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119
           KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG
Sbjct: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119

BLAST of MC04g1033 vs. NCBI nr
Match: XP_022962902.1 (non-specific lipid transfer protein GPI-anchored 2 [Cucurbita moschata] >KAG6601904.1 hypothetical protein SDJN03_07137, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032603.1 hypothetical protein SDJN02_06652 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 175 bits (444), Expect = 4.21e-54
Identity = 91/119 (76.47%), Postives = 102/119 (85.71%), Query Frame = 0

Query: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60
           MASFS+ SK LIVA   +ILVASEGFI  AGAAGECGK  V  AA+ LTPCL A +D RA
Sbjct: 1   MASFSSVSKPLIVAV-FVILVASEGFIAPAGAAGECGKTPVESAALGLTPCLAATRDARA 60

Query: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119
           KV+ ACCS+VA MFNT+PKCLCAILLSP++KQAGINPA+AITIPKRCNI+NRPAGKKCG
Sbjct: 61  KVSPACCSRVAAMFNTSPKCLCAILLSPVSKQAGINPAIAITIPKRCNIRNRPAGKKCG 118

BLAST of MC04g1033 vs. NCBI nr
Match: XP_022989841.1 (non-specific lipid transfer protein GPI-anchored 2 [Cucurbita maxima])

HSP 1 Score: 174 bits (442), Expect = 8.76e-54
Identity = 88/119 (73.95%), Postives = 101/119 (84.87%), Query Frame = 0

Query: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60
           MASFS+ SK  ++ A  +ILVASEGFI RA AAGECGK  V  AA+ LTPCL A +D RA
Sbjct: 1   MASFSSVSKPPLIVAVFVILVASEGFIARARAAGECGKTPVESAALGLTPCLAATRDARA 60

Query: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119
           KV+ ACCS+VA MFNT+PKCLCAILLSP++KQAGINPA+AITIPKRCNI+NRPAGKKCG
Sbjct: 61  KVSPACCSRVAAMFNTSPKCLCAILLSPVSKQAGINPAIAITIPKRCNIRNRPAGKKCG 119

BLAST of MC04g1033 vs. NCBI nr
Match: XP_023516114.1 (non-specific lipid transfer protein GPI-anchored 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 173 bits (438), Expect = 3.45e-53
Identity = 90/119 (75.63%), Postives = 101/119 (84.87%), Query Frame = 0

Query: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60
           MASFS+ S  LIVA   +ILVASEGFI  AGAAGECGK  V  AA+ LTPCL A +D RA
Sbjct: 1   MASFSSVSTPLIVAV-FVILVASEGFIAPAGAAGECGKTPVESAALGLTPCLAATRDARA 60

Query: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119
           KV+ ACCS+VA MFNT+PKCLCAILLSP++KQAGINPA+AITIPKRCNI+NRPAGKKCG
Sbjct: 61  KVSPACCSRVAAMFNTSPKCLCAILLSPVSKQAGINPAIAITIPKRCNIRNRPAGKKCG 118

BLAST of MC04g1033 vs. NCBI nr
Match: XP_022146338.1 (uncharacterized protein LOC111015576 [Momordica charantia])

HSP 1 Score: 166 bits (421), Expect = 1.39e-50
Identity = 83/119 (69.75%), Postives = 95/119 (79.83%), Query Frame = 0

Query: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60
           MAS ++T KAL+VAAG LILVASE FIH A AAG CGKMT+G AA SL  C GAV DV+A
Sbjct: 1   MASSNSTCKALVVAAGFLILVASEAFIHPAAAAGVCGKMTIGSAAYSLISCTGAVADVKA 60

Query: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119
           KV+  CCS+VA M  TTPKC CAILL P  ++ G+NPA+ +TIPKRCNIKNRPAGKKCG
Sbjct: 61  KVSPECCSRVAAMLKTTPKCFCAILLFPKTRKQGVNPAIGLTIPKRCNIKNRPAGKKCG 119

BLAST of MC04g1033 vs. ExPASy TrEMBL
Match: A0A6J1CY97 (non-specific lipid transfer protein GPI-anchored 2-like OS=Momordica charantia OX=3673 GN=LOC111015552 PE=3 SV=1)

HSP 1 Score: 230 bits (587), Expect = 3.33e-76
Identity = 119/119 (100.00%), Postives = 119/119 (100.00%), Query Frame = 0

Query: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60
           MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA
Sbjct: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60

Query: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119
           KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG
Sbjct: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119

BLAST of MC04g1033 vs. ExPASy TrEMBL
Match: A0A6J1HDU2 (non-specific lipid transfer protein GPI-anchored 2 OS=Cucurbita moschata OX=3662 GN=LOC111463265 PE=4 SV=1)

HSP 1 Score: 175 bits (444), Expect = 2.04e-54
Identity = 91/119 (76.47%), Postives = 102/119 (85.71%), Query Frame = 0

Query: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60
           MASFS+ SK LIVA   +ILVASEGFI  AGAAGECGK  V  AA+ LTPCL A +D RA
Sbjct: 1   MASFSSVSKPLIVAV-FVILVASEGFIAPAGAAGECGKTPVESAALGLTPCLAATRDARA 60

Query: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119
           KV+ ACCS+VA MFNT+PKCLCAILLSP++KQAGINPA+AITIPKRCNI+NRPAGKKCG
Sbjct: 61  KVSPACCSRVAAMFNTSPKCLCAILLSPVSKQAGINPAIAITIPKRCNIRNRPAGKKCG 118

BLAST of MC04g1033 vs. ExPASy TrEMBL
Match: A0A6J1JLA4 (non-specific lipid transfer protein GPI-anchored 2 OS=Cucurbita maxima OX=3661 GN=LOC111486908 PE=4 SV=1)

HSP 1 Score: 174 bits (442), Expect = 4.24e-54
Identity = 88/119 (73.95%), Postives = 101/119 (84.87%), Query Frame = 0

Query: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60
           MASFS+ SK  ++ A  +ILVASEGFI RA AAGECGK  V  AA+ LTPCL A +D RA
Sbjct: 1   MASFSSVSKPPLIVAVFVILVASEGFIARARAAGECGKTPVESAALGLTPCLAATRDARA 60

Query: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119
           KV+ ACCS+VA MFNT+PKCLCAILLSP++KQAGINPA+AITIPKRCNI+NRPAGKKCG
Sbjct: 61  KVSPACCSRVAAMFNTSPKCLCAILLSPVSKQAGINPAIAITIPKRCNIRNRPAGKKCG 119

BLAST of MC04g1033 vs. ExPASy TrEMBL
Match: A0A6J1CYB6 (uncharacterized protein LOC111015576 OS=Momordica charantia OX=3673 GN=LOC111015576 PE=4 SV=1)

HSP 1 Score: 166 bits (421), Expect = 6.71e-51
Identity = 83/119 (69.75%), Postives = 95/119 (79.83%), Query Frame = 0

Query: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60
           MAS ++T KAL+VAAG LILVASE FIH A AAG CGKMT+G AA SL  C GAV DV+A
Sbjct: 1   MASSNSTCKALVVAAGFLILVASEAFIHPAAAAGVCGKMTIGSAAYSLISCTGAVADVKA 60

Query: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119
           KV+  CCS+VA M  TTPKC CAILL P  ++ G+NPA+ +TIPKRCNIKNRPAGKKCG
Sbjct: 61  KVSPECCSRVAAMLKTTPKCFCAILLFPKTRKQGVNPAIGLTIPKRCNIKNRPAGKKCG 119

BLAST of MC04g1033 vs. ExPASy TrEMBL
Match: A0A0A0KJJ0 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G099500 PE=4 SV=1)

HSP 1 Score: 159 bits (403), Expect = 3.26e-48
Identity = 80/119 (67.23%), Postives = 94/119 (78.99%), Query Frame = 0

Query: 1   MASFSTTSKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRA 60
           M SFS  SK +IV   +++LV     +   GAAGECGK  +  AA+ LTPCLGAV+DV+A
Sbjct: 2   MGSFSIASKGVIVGMFVIVLV-----VWEVGAAGECGKTPIESAAMGLTPCLGAVRDVKA 61

Query: 61  KVTAACCSKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 119
           KVT ACCSKV  MFN++PKCLCAILLSP+AKQAGINP +AITIPKRCNI+NRP GKKCG
Sbjct: 62  KVTGACCSKVGAMFNSSPKCLCAILLSPLAKQAGINPGIAITIPKRCNIRNRPKGKKCG 115

BLAST of MC04g1033 vs. TAIR 10
Match: AT2G37870.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 124.0 bits (310), Expect = 8.2e-29
Identity = 60/107 (56.07%), Postives = 78/107 (72.90%), Query Frame = 0

Query: 13  VAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRAKVTAACCSKVAG 72
           VA  L+ L+ S   +    AAGECG+M +  AA SL+PCL A K+ R KV   CC+KV  
Sbjct: 7   VAVALMSLLISLASVE---AAGECGRMPINQAAASLSPCLPATKNPRGKVPPVCCAKVGA 66

Query: 73  MFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCG 120
           +  T P+CLCA++LSP+AK+AGINP +AI +PKRCNI+NRPAGK+CG
Sbjct: 67  LIRTNPRCLCAVMLSPLAKKAGINPGIAIGVPKRCNIRNRPAGKRCG 110

BLAST of MC04g1033 vs. TAIR 10
Match: AT3G53980.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 95.5 bits (236), Expect = 3.1e-20
Identity = 50/113 (44.25%), Postives = 69/113 (61.06%), Query Frame = 0

Query: 8   SKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRAKVTAACC 67
           S   +   GL++L+ +         AGECG+ +    A+ L PC GA +D  + V   CC
Sbjct: 4   SMKFLCILGLILLIGTV-----VDGAGECGRSSPDNEAMKLAPCAGAAQDANSAVPGGCC 63

Query: 68  SKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCGS 121
           +++   F+  PKCLCAILLS  AK +G++P VA+TIPKRCN  NRP G KCG+
Sbjct: 64  TQIK-RFSQNPKCLCAILLSDTAKASGVDPEVALTIPKRCNFANRPVGYKCGA 110

BLAST of MC04g1033 vs. TAIR 10
Match: AT3G53980.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 95.5 bits (236), Expect = 3.1e-20
Identity = 50/113 (44.25%), Postives = 69/113 (61.06%), Query Frame = 0

Query: 8   SKALIVAAGLLILVASEGFIHRAGAAGECGKMTVGLAAVSLTPCLGAVKDVRAKVTAACC 67
           S   +   GL++L+ +         AGECG+ +    A+ L PC GA +D  + V   CC
Sbjct: 4   SMKFLCILGLILLIGTV-----VDGAGECGRSSPDNEAMKLAPCAGAAQDANSAVPGGCC 63

Query: 68  SKVAGMFNTTPKCLCAILLSPMAKQAGINPAVAITIPKRCNIKNRPAGKKCGS 121
           +++   F+  PKCLCAILLS  AK +G++P VA+TIPKRCN  NRP G KCG+
Sbjct: 64  TQIK-RFSQNPKCLCAILLSDTAKASGVDPEVALTIPKRCNFANRPVGYKCGA 110

BLAST of MC04g1033 vs. TAIR 10
Match: AT5G05960.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.2e-19
Identity = 45/88 (51.14%), Postives = 59/88 (67.05%), Query Frame = 0

Query: 33  AGECGKMTVGLAAVSLTPCLGAVKDVRAKVTAACCSKVAGMFNTTPKCLCAILLSPMAKQ 92
           AGECG+      A+ L PC  A +D  AKV+A CC++V  M    PKCLCA++LS  A+ 
Sbjct: 26  AGECGRNPPDREAIKLAPCAMAAQDTSAKVSAICCARVKQM-GQNPKCLCAVMLSSTARS 85

Query: 93  AGINPAVAITIPKRCNIKNRPAGKKCGS 121
           +G  P +++TIPKRCNI NRP G KCG+
Sbjct: 86  SGAKPEISMTIPKRCNIANRPVGYKCGA 112

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022146313.16.88e-76100.00non-specific lipid transfer protein GPI-anchored 2-like [Momordica charantia][more]
XP_022962902.14.21e-5476.47non-specific lipid transfer protein GPI-anchored 2 [Cucurbita moschata] >KAG6601... [more]
XP_022989841.18.76e-5473.95non-specific lipid transfer protein GPI-anchored 2 [Cucurbita maxima][more]
XP_023516114.13.45e-5375.63non-specific lipid transfer protein GPI-anchored 2 [Cucurbita pepo subsp. pepo][more]
XP_022146338.11.39e-5069.75uncharacterized protein LOC111015576 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1CY973.33e-76100.00non-specific lipid transfer protein GPI-anchored 2-like OS=Momordica charantia O... [more]
A0A6J1HDU22.04e-5476.47non-specific lipid transfer protein GPI-anchored 2 OS=Cucurbita moschata OX=3662... [more]
A0A6J1JLA44.24e-5473.95non-specific lipid transfer protein GPI-anchored 2 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1CYB66.71e-5169.75uncharacterized protein LOC111015576 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
A0A0A0KJJ03.26e-4867.23AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G099500 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT2G37870.18.2e-2956.07Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT3G53980.13.1e-2044.25Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT3G53980.23.1e-2044.25Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT5G05960.11.2e-1951.14Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000528Plant non-specific lipid-transfer protein/Par allergenPRINTSPR00382LIPIDTRNSFERcoord: 40..56
score: 28.24
coord: 94..111
score: 31.54
coord: 61..75
score: 29.44
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilyGENE3D1.10.110.10coord: 39..115
e-value: 9.5E-15
score: 56.2
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilySUPERFAMILY47699Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumincoord: 42..113
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainPFAMPF14368LTP_2coord: 32..118
e-value: 1.0E-14
score: 54.4
NoneNo IPR availablePANTHERPTHR33122:SF13NON-SPECIFIC LIPID-TRANSFER PROTEIN 4.1coord: 9..120
NoneNo IPR availableCDDcd00010AAI_LTSScoord: 48..109
e-value: 3.05553E-13
score: 58.2071
IPR039265Putative lipid-transfer protein DIR1-likePANTHERPTHR33122LIPID BINDING PROTEIN-RELATEDcoord: 9..120

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g1033.1MC04g1033.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006869 lipid transport
biological_process GO:0009627 systemic acquired resistance
molecular_function GO:0005504 fatty acid binding
molecular_function GO:0008289 lipid binding