Homology
BLAST of MC04g1001 vs. ExPASy Swiss-Prot
Match:
Q9LQV2 (RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1)
HSP 1 Score: 1230.7 bits (3183), Expect = 0.0e+00
Identity = 643/1110 (57.93%), Postives = 802/1110 (72.25%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
MGKTI+++GF V+AEEVK+FLE TG GTV A+++ + + R +A VQF+SE
Sbjct: 1 MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPR-VYAIVQFTSERHT 60
Query: 61 GHVVSQAAAEERLWYGSSYLKAREADRQ-NRRGGVGVERMENLTLHLGSQISKEKLSVIW 120
+++ AA ERL+YG SYLKA E ++ + + + L + G Q+S +K +W
Sbjct: 61 RLIIT--AAAERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLW 120
Query: 121 KRENCSVEFGSGVRKL---FVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAP 180
++ V FG G+RKL F + +Y++E+ +E+I ++ L P +SKFL+IQ+ AP
Sbjct: 121 SAQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIGAP 180
Query: 181 RIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ 240
+IF+K + FY S S +WIR DFT SSCIGQS A CLEL
Sbjct: 181 KIFEKEDQPINLLFGIMD--FY---SDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLN 240
Query: 241 LPSFFKTLVGY-EEIYGPFNLETGSSFSSTSN-LVPIVTPPQGFDFSFKILFKINALVQH 300
+P F + Y E F +E+GSS+SS +N LVP+V PP GF F+ILFK+N LVQ+
Sbjct: 241 VPDFRENFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQN 300
Query: 301 GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQL 360
L GP LD +F+RL++ ++ I+ LEKLF+L EC YEP WL+ +Y + + +L
Sbjct: 301 ACLSGPALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKL 360
Query: 361 PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 420
P P ISLDDGLVY++RVQ+TP++VYF GPE N+SNRV+R YS I+NF+RVSFVDE+L+
Sbjct: 361 PLSPTISLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLE 420
Query: 421 KIRSIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 480
K+RS+DLSPR+ST RT++YDRI S LR+GIVIGDKKFEFLAFS+SQLRENS WMFA
Sbjct: 421 KVRSMDLSPRSSTQ--RRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAP 480
Query: 481 REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 540
+ ++AA IR WMGDF IRNVAKYAARLGQSF SSR+TL V EIEVI DVE+ +
Sbjct: 481 IDRITAAHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGT 540
Query: 541 TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSM 600
Y FSDGIGKIS A KVA +CGL +PSAFQIRY GYKGVVA+DP S KKLSLRKSM
Sbjct: 541 RYVFSDGIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSM 600
Query: 601 LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPS 660
K++S +T+LDVLAWSKY PC++NRQ+I LLSTLG+ D VFEKKQ+E + +L+ IL P
Sbjct: 601 SKFESENTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPL 660
Query: 661 RALEVLELMSPGEMTSVLKEMLLY-YKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKG 720
A E L LM+PGE T++LK ++L YKP+ EPFL+MML+ FRA KLL+LRTK+RIF+ G
Sbjct: 661 EAHEALGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGG 720
Query: 721 RTMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDV 780
R+MMGCLDET TL+YGQV VQ S P R FI G VVVAKNPCLHPGD+RVL AV+V
Sbjct: 721 RSMMGCLDETRTLEYGQVVVQYSDPMRPGR-RFIITGPVVVAKNPCLHPGDVRVLQAVNV 780
Query: 781 KALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQ 840
AL+HMVDCVVFPQKG RPHPNECSGSDLDGD+YFVCWD EL R +PM Y P PT
Sbjct: 781 PALNHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQI 840
Query: 841 LDHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFP 900
LDHDVTIEEV+EY ANY+VND LG IANAHT FAD+EP KA S CI+LAK FS AVDFP
Sbjct: 841 LDHDVTIEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFP 900
Query: 901 KTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVA 960
KTGV A +P L V E+PDFMEK D+P YESK VIGKLFR VK+ + +I+++T +VA
Sbjct: 901 KTGVAAVIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVA 960
Query: 961 AKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDK 1020
+K YD DME+DGFE+Y++EAF K YD+KLGNLMDYYGI+TE E++SG I+R+ KSF K
Sbjct: 961 SKSYDKDMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTK 1020
Query: 1021 RTDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWG 1080
R D E I A+R+LRKE + FN + +E+ E AKASAWYHVTYH SYWG
Sbjct: 1021 RRDAESIGRAVRALRKETLSLFNA-----------SEEEENESAKASAWYHVTYHSSYWG 1080
Query: 1081 RYNEGMERDHFLSFPWCVSDKLIQIKKKKI 1104
YNEG+ RDHFLSF WCV DKL++IKK +
Sbjct: 1081 LYNEGLNRDHFLSFAWCVYDKLVRIKKTNL 1088
BLAST of MC04g1001 vs. ExPASy Swiss-Prot
Match:
Q0DXS3 (Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR1 PE=2 SV=2)
HSP 1 Score: 924.1 bits (2387), Expect = 1.5e-267
Identity = 456/728 (62.64%), Postives = 573/728 (78.71%), Query Frame = 0
Query: 377 SKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSIDLSPRTSTA-NGERTRV 436
S VYF GPE N+SNRVVR +S DI+NF+R+SFVDE+ +K+R+ DLSPR+++ + RT +
Sbjct: 7 STVYFYGPEINVSNRVVRNFSSDIENFLRISFVDEDCEKLRATDLSPRSASGHDANRTAL 66
Query: 437 YDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSAADIREWMGDFRQIRN 496
Y R+LS L +GI IG K FEFLAFS+SQLR+NS WMFASR+GL+A+DIR WMGDFR IRN
Sbjct: 67 YKRVLSVLSDGITIGGKNFEFLAFSSSQLRDNSAWMFASRQGLAASDIRTWMGDFRNIRN 126
Query: 497 VAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSDGIGKISETLAEKVAE 556
VAKYAARLGQSF SS +TL V+++E+E I+D++ N + FSDGIGKIS A +VA
Sbjct: 127 VAKYAARLGQSFSSSTETLKVQKYEVEEISDIK---NGTQHVFSDGIGKISSAFANEVAM 186
Query: 557 RCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLKYKSLDTQLDVLAWSKYLPC 616
+C L PSAFQIRY GYKGVVA+DPTS KLSLRKSMLK++S + +DVLA+SKY P
Sbjct: 187 KCNLKRFAPSAFQIRYGGYKGVVAVDPTSRWKLSLRKSMLKFQSDNITVDVLAYSKYQPG 246
Query: 617 FLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVLELMSPGEMTSVLKEM 676
FLNRQ+I LLSTLG++D VFE+KQ+EA+ QLN ++ DP A+E +ELM GE+T+ +KE+
Sbjct: 247 FLNRQLITLLSTLGVRDSVFEQKQEEAVNQLNKMVTDPQAAIEAIELMPMGEITNAVKEL 306
Query: 677 LLY-YKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETGTLKYGQVFVQ 736
LL Y+P+ EP+L+M+L+TFRA KLL+L+TKSRI +PKGR MMGCLDET TLKYGQVF++
Sbjct: 307 LLCGYQPDDEPYLSMLLQTFRASKLLELKTKSRILIPKGRAMMGCLDETRTLKYGQVFIR 366
Query: 737 CSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMVDCVVFPQKGQRPHP 796
+ G + F GKVV+AKNPCLHPGD+R+L+AVDV LHHM +CVVFPQ+G RPHP
Sbjct: 367 -ATSGVNDNDRFTVTGKVVIAKNPCLHPGDIRILHAVDVPVLHHMFNCVVFPQQGPRPHP 426
Query: 797 NECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTIEEVQEYMANYMVND 856
NECSGSDLDGD+YFV WDP L R V PM Y PAPT LDHDVTIEEV+EY NY+VN+
Sbjct: 427 NECSGSDLDGDIYFVSWDPSLIPPRMVTPMDYTPAPTETLDHDVTIEEVEEYFTNYIVNE 486
Query: 857 GLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPANLPPFLRVHEFPDFM 916
LG IANAH VFAD+E KA S+ CI+LAKLFSIAVDFPKTGVPA +PP L V E+PDFM
Sbjct: 487 SLGMIANAHVVFADKEDLKAESSPCIELAKLFSIAVDFPKTGVPALIPPELHVKEYPDFM 546
Query: 917 EKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPDMEIDGFEKYLNEAF 976
EKLD+ YESKGVIGKL+R +K +T I+ +TREVA + YD DM +DG+E Y+ EA
Sbjct: 547 EKLDKVTYESKGVIGKLYREIKK---HTPHIKHFTREVARRSYDTDMIVDGYEDYITEAM 606
Query: 977 DFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQISHAMRSLRKEARAW 1036
K YD+KLGNLMD+YGI++E E++SG IL++ K+F K++D + I A+RSLRKEAR+
Sbjct: 607 ALKDEYDFKLGNLMDHYGIKSEAEIISGCILKMAKNFTKKSDADAIRLAVRSLRKEARSR 666
Query: 1037 FNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGMERDHFLSFPWCVSDK 1096
F+E DD+ +G + E AKASAWYHVTYH +WG YNEG ER HF+SFPWC+ +K
Sbjct: 667 FSEMS----LDDNGHGHDASE-AKASAWYHVTYHPEFWGCYNEGYERPHFISFPWCIYEK 722
Query: 1097 LIQIKKKK 1103
L++IK+++
Sbjct: 727 LLRIKQRR 722
BLAST of MC04g1001 vs. ExPASy Swiss-Prot
Match:
O82504 (RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1)
HSP 1 Score: 734.2 bits (1894), Expect = 2.2e-210
Identity = 447/1140 (39.21%), Postives = 657/1140 (57.63%), Query Frame = 0
Query: 4 TIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAAGHV 63
T++I + + A+E+ FLE H G+ TV A+ I RD FA VQF++ L
Sbjct: 11 TVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTT-LEVKSR 70
Query: 64 VSQAAAEERLWYGSSYLKAREA-DRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWKRE 123
+++ +L + + L+ EA D R +R++++ L +G S EK
Sbjct: 71 AQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEK-------R 130
Query: 124 NCSVEFGSGVR----------KLFVYVNLE-YKMEVWFESILRVQLRCPPDGASKF--LL 183
C++E GVR + +V+ + + YK+EV FE I+ C AS+ L
Sbjct: 131 FCALEKWDGVRCWILTEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFL 190
Query: 184 IQLQRAPRIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCL 243
++L+ P++FK+ + ++ S RF + WIR DF+ S IG S CL
Sbjct: 191 LKLKYGPKVFKRVTVHIATKFKSDRYRFC---KEDFDFMWIRTTDFSGSKSIGTSTCFCL 250
Query: 244 ELSPGDQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPP-QGFDFSFKILFKI 303
E+ G + F L Y E G +F+S + +VP++ G +F ++ILF++
Sbjct: 251 EVHNGSTMLDIFSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQL 310
Query: 304 NALVQHGYLP-GPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLS 363
NALV + D E +++ + L+KL Y+P ++K Q S
Sbjct: 311 NALVHAQKISLFAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQS 370
Query: 364 YYNSKQLPWKPDIS----LDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNF 423
K++ P + + ++ R +TPSK+Y GPE +N VV+ +++ + +F
Sbjct: 371 VV--KKMKHSPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDF 430
Query: 424 IRVSFVDEELDKIRSIDLSPRTST---ANGERTRVYDRILSTLRNGIVIGDKKFEFLAFS 483
+RV+FV+E+ K+ + LS + RT +Y+R+LS L GI +G K+FEFLAFS
Sbjct: 431 MRVTFVEEDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFS 490
Query: 484 ASQLRENSVWMFASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHE 543
ASQLR NSVWMFAS E + A DIREWMG FR+IR+++K AAR+GQ F +SR+TL V +
Sbjct: 491 ASQLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQD 550
Query: 544 IEVITDVEVETNNVTYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAI 603
+E I D+EV T+ YCFSDGIGKIS A++VA++CGL +H PSAFQIRY GYKGV+A+
Sbjct: 551 VEQIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQKCGL-SHVPSAFQIRYGGYKGVIAV 610
Query: 604 DPTSEKKLSLRKSMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQK 663
D +S +KLSLR SMLK+ S + L+V W++ +PCFLNR++I LLSTLGI+D +FE Q
Sbjct: 611 DRSSFRKLSLRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQA 670
Query: 664 EAIYQLNCILKDPSRALEVLELMSPGEMTSVLKEMLLY-YKPNKEPFLNMMLRTFRADKL 723
+ L +L+D AL VL+ +S ++L +MLL Y P+ EP+L+MMLR +L
Sbjct: 671 VHLSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQL 730
Query: 724 LDLRTKSRIFVPKGRTMMGCLDETGTLKYGQVFVQCSVP------------GRSSESNFI 783
+L+++ RI VPKGR ++GC+DE G L+YGQV+V+ ++ + E +
Sbjct: 731 SELKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSV 790
Query: 784 PEGKVVVAKNPCLHPGDMRVLNAVDVKALHH--MVDCVVFPQKGQRPHPNECSGSDLDGD 843
GKVVV KNPCLHPGD+RVL+A+ +DC++FPQKG+RPHPNECSG DLDGD
Sbjct: 791 VIGKVVVTKNPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGD 850
Query: 844 LYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTV 903
+FV WD ++ PM Y + +DHDVT+EE+ ++ +YM++D LG I+ AH V
Sbjct: 851 QFFVSWDEKIIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLV 910
Query: 904 FADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESK 963
ADR+P KA S KC++LA L S AVDF KTG PA +P L+ EFPDF+E+ ++P Y S+
Sbjct: 911 HADRDPEKARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISE 970
Query: 964 GVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLG 1023
V GKL+R+VK S E + + A YD +E GFE ++ A + Y KL
Sbjct: 971 SVFGKLYRAVKS-SLAQRKPEAESEDTVA--YDVTLEEAGFESFIETAKAHRDMYGEKLT 1030
Query: 1024 NLMDYYGIRTEPELVSGNILRVGKSFDKRTD------MEQISHAMRSLRKEARAWFNEKG 1083
+LM YYG E E+++G IL+ + + R + ++I+ +++ L KEA WF +
Sbjct: 1031 SLMIYYGAANEEEILTG-ILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWFEKS- 1090
Query: 1084 SKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGMERDHFLSFPWCVSDKLIQIK 1100
C+ DE ++ ASAWY+VTY+ N E+ FLSFPW V D L+ IK
Sbjct: 1091 ----CE-----DEQQKKKLASAWYYVTYNP------NHRDEKLTFLSFPWIVGDVLLDIK 1114
BLAST of MC04g1001 vs. ExPASy Swiss-Prot
Match:
Q7XM31 (Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR2 PE=2 SV=1)
HSP 1 Score: 695.7 bits (1794), Expect = 8.7e-199
Identity = 418/1087 (38.45%), Postives = 625/1087 (57.50%), Query Frame = 0
Query: 51 TVQFSSELAAGHVVSQAAAEERLWYGSSYLKA---------READRQNRRGGVGVERMEN 110
TVQF S AA A++ + + L R +D R G G
Sbjct: 59 TVQFGSAAAAAAAAGLASSGRLPRFLGALLSVSPSPVDLLPRASDLSLRAAGAG------ 118
Query: 111 LTLHLGSQISKEKLSVIWKRENCSVEFGSGVRKLFVYV---NLEYKMEVWFESILRVQLR 170
L +G ++++ + E G R++ +Y+ + YK+EV FE ++ L
Sbjct: 119 --LVVGDRVAERVFEAADAWDGVRAEVIPGKRRVDLYLEHDSQRYKLEVLFED-MKDCLG 178
Query: 171 CPPDGASKFLLIQLQRAPRIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPS 230
C DG +L+QL APRI + S + +S RF+ + + S W+R +DFTP+
Sbjct: 179 CTLDGMGA-ILLQLNYAPRI-HTAISGPAVNSRFMDDRFHACKEDAKFS-WVRALDFTPN 238
Query: 231 SCIGQSFALCLELSPGDQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGF 290
G+ L L+L + K+L + G + + ++SN+VP+V P+ +
Sbjct: 239 YSFGRCSTLVLKLGKSALVSDILKSL-PFSGNLGELTMNSMDGVGASSNVVPLVHCPRDY 298
Query: 291 DFSFKILFKINALVQHGYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQ 350
+++LF++N+L+ G + ++ + F+ + + R EK+ L+ Y P
Sbjct: 299 SVPYEVLFRLNSLMHMGKIVAKHVNADLFKAL--QELPVDVSRRIFEKMHKLESTCYGPL 358
Query: 351 KWLKQQYLSYYNSKQ--LPWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQY 410
++++Q+ S S L + + + L+ +RV ITPSK++ GPE ++N VV+ +
Sbjct: 359 QFIQQEAYSMKRSHNVLLSNEGEGEGERKLMKCYRVNITPSKIFCFGPEEEVTNYVVKHH 418
Query: 411 SDDIDNFIRVSFVDEELDKIRSIDLSPRTST---ANGERTRVYDRILSTLRNGIVIGDKK 470
S +F+RV+FVDE+ K+ S +S R + +T +Y RILS L+ G IG K
Sbjct: 419 SAYASDFVRVTFVDEDWSKLSSNAISARIEQGFFSKPFKTGLYYRILSILKKGFSIGPKN 478
Query: 471 FEFLAFSASQLRENSVWMFASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKT 530
FEFLAFSASQLR NSVWMFAS L+A IR WMG F IR+V+K AAR+GQ F SSR+T
Sbjct: 479 FEFLAFSASQLRGNSVWMFASNASLNAGGIRRWMGHFENIRSVSKCAARMGQLFSSSRQT 538
Query: 531 LCVEEHEIEVITDVEVETNNVTYCFSDGIGKISETLAEKVAERCGL-INHTPSAFQIRYA 590
V ++EVI D+E+ T+ Y FSDGIGKIS A++VA GL + PSAFQIRY
Sbjct: 539 FEVLRWDVEVIPDIEITTDGSKYIFSDGIGKISLRFAKRVAHHVGLDPTNLPSAFQIRYG 598
Query: 591 GYKGVVAIDPTSEKKLSLRKSMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKD 650
GYKGV+AIDP S LSLR SM K++S L++ +WSK PC++NR++I+LLSTLGI+D
Sbjct: 599 GYKGVIAIDPMSSIDLSLRPSMKKFESESRMLNITSWSKSQPCYVNREIISLLSTLGIRD 658
Query: 651 RVFEKKQKEAIYQLNCILKDPSRALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLR 710
+F Q++ + + +L + AL VL + E + +K +L Y+P+ EP+L+M+L+
Sbjct: 659 EIFVAMQQDEMRETEEMLTNKEVALSVLGKLGGSETKTAVKMLLQGYEPSSEPYLSMILK 718
Query: 711 TFRADKLLDLRTKSRIFVPKGRTMMGCLDETGTLKYGQVFVQCSVPGR----SSESNFIP 770
+ ++L D+RT+ +I VPKGR ++GCLDETG L+YGQV+++ + + S++S F
Sbjct: 719 AHQENRLTDIRTRCKIHVPKGRVLIGCLDETGVLEYGQVYIRITKNSKEQKDSNQSYFYN 778
Query: 771 E--------GKVVVAKNPCLHPGDMRVLNAVDVKALHHMVDCVVFPQKGQRPHPNECSGS 830
+ GKV + KNPCLHPGD+RVL A+ L MVDC+VFPQ+G+RPHPNECSG
Sbjct: 779 DDGKTATVVGKVAITKNPCLHPGDIRVLEAIYDPDLVGMVDCLVFPQRGERPHPNECSGG 838
Query: 831 DLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTIEEVQEYMANYMVNDGLGAIA 890
DLDGDLYF+ WD +L + PM Y +DH VT+EE+Q++ +YM+ND LGAI+
Sbjct: 839 DLDGDLYFITWDDKLIPEKVDTPMDYTATRPRIMDHVVTLEEIQKHFVDYMINDSLGAIS 898
Query: 891 NAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPANLPPFLRVHEFPDFMEKLDRP 950
AH + ADR P KA S +C++LA L S+AVDF KTG PA +P LR E+PDFME+ ++P
Sbjct: 899 TAHLIHADRSPLKARSPECLQLATLHSMAVDFAKTGAPAEMPRTLRPREYPDFMERWEKP 958
Query: 951 AYESKGVIGKLFRSV---KDVSFNTNTIETYTREVAAKCYDPDMEIDGFEKYLNEAFDFK 1010
Y S GV+GKL+RS + S ++ + + + + + YDPD+E+ G +++L A ++
Sbjct: 959 MYISNGVLGKLYRSAMGHMEKSGDSGALSSSSAQ-PSPTYDPDLEVPGSDEFLQAAEEYY 1018
Query: 1011 TRYDYKLGNLMDYYGIRTEPELVSGNI----LRVGKSFDKRTDM-EQISHAMRSLRKEAR 1070
Y+ KL LM+YY E E+++GNI L + + + +M ++I A+ +L +EAR
Sbjct: 1019 ELYEEKLTTLMNYYRAELEDEILTGNIRNKMLYLKRDNKRYFEMKDRIVAAVDALHREAR 1078
Query: 1071 AWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGMERDHFLSFPWCVS 1100
W + E++ ASAWY VTYH + F SFPW
Sbjct: 1079 GWLL-----------SSRKEEDASRMASAWYRVTYHP-------DRRRGKRFWSFPWIAC 1111
BLAST of MC04g1001 vs. ExPASy Swiss-Prot
Match:
Q9SG02 (RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1)
HSP 1 Score: 565.1 bits (1455), Expect = 1.8e-159
Identity = 383/1068 (35.86%), Postives = 566/1068 (53.00%), Query Frame = 0
Query: 98 RMENLTLHLGSQISKEKLSVIWKRENC-----------------SVEFGSGVRKLFVYVN 157
++ +TL +G+ +S++ V W+ E S F + +N
Sbjct: 131 KLAGITLEIGTLVSRDDFFVSWRAEGVDFLVDPFDNTCKFCFRKSTAFSFKDAVMHAVIN 190
Query: 158 LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAPRIFKKSSSSSSSSSSSSSSRFYFK 217
+YK+E+ I V+ G L++QL +PR++ +++ +
Sbjct: 191 CDYKLELLVRDIQTVRQYKTLHGF--VLILQLASSPRVWYRTADDDIYDTVPG------- 250
Query: 218 ESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ------LPSFFKTLVGYEEIYGPF 277
+ WIR DFT IG+ + + +SP + L F V E + P
Sbjct: 251 DLLDDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERVRWPP 310
Query: 278 NLETGSSFSS-TSNLVPIVTPPQGFDFSFKILFKINALVQHGYLPGPLLDDEFFRLVDPS 337
+ F S+ + +G SF+I+F +N+++ G L + FF L+
Sbjct: 311 RIRNEPCFGEPVSDHFFCIHHKEG--ISFEIMFLVNSVLHRGVFNQFQLTERFFDLL--- 370
Query: 338 RFRPEYIERA-LEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPWKPDISLDDGLVYVHRV 397
R +P+ + A L+ L K ++ K LK + +L + S D + + R+
Sbjct: 371 RNQPKDVNIASLKHLCTYKRPVFDAYKRLKLVQEWIQKNPKLLGSHEQSED--ISEIRRL 430
Query: 398 QITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSIDLS--------PR 457
ITP++ Y PE LSNRV+R+Y + F+RV+F+DE + I S LS
Sbjct: 431 VITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAPIVKDL 490
Query: 458 TSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSAADIR 517
TS++ ++T V+ R+ S L +G + +K+ FLAFSA+QLR+ S W FA +DI+
Sbjct: 491 TSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTRVSDIK 550
Query: 518 EWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDV-EVETNNVTYCFSDGIG 577
WMG F+ +NVAK AAR+G F S+ T+ V HE++ T+V ++E N Y FSDGIG
Sbjct: 551 TWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVD--TEVPDIERNG--YVFSDGIG 610
Query: 578 KISETLAEKVAERCGL-INHTPSAFQIRYAGYKGVVAIDPTSEK--KLSLRKSMLKYKSL 637
I+ LA++V E+ L ++++P A+QIRYAG+KGVVA P+ +L+LR SM K+ S
Sbjct: 611 TITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSMKKFFSK 670
Query: 638 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVL 697
T L++ +W+++ P FLNRQ+I LLS LG+ D +F Q+ +Y+LN IL D A EVL
Sbjct: 671 HTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTDVAFEVL 730
Query: 698 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 757
+ + + +KP EP L ML + R +L LR KSRIFV GR +MGCL
Sbjct: 731 TASCAEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSGRWLMGCL 790
Query: 758 DETGTLKYGQVFVQCSVP----------GRSSESNF---IPEGKVVVAKNPCLHPGDMRV 817
DE G L++GQ F+Q S P R E+ + +G V +AKNPCLHPGD+R+
Sbjct: 791 DEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDLEVVKGYVAIAKNPCLHPGDVRI 850
Query: 818 LNAVDVKALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVK----P 877
L AVDV LHHM DC++FPQKG RPH NE SGSDLDGDLYFV WD +L I P +
Sbjct: 851 LEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKL--IPPNRKSYPA 910
Query: 878 MSYKPAPTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLA 937
M Y A L V +++ ++ A + N+ LG I NAH V ADR AM +C+ LA
Sbjct: 911 MHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDEECLLLA 970
Query: 938 KLFSIAVDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDV-SFNT 997
+L + AVDFPKTG ++P L+ +PDFM K D Y+S ++G+L+R VK+V +
Sbjct: 971 ELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKEVYDEDA 1030
Query: 998 NTIETYTREVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSG 1057
+ + +A YD +EI GFE + EA+ K YD +L L+ Y ++ E E+V+G
Sbjct: 1031 EASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKEEEIVTG 1090
Query: 1058 NILRVGKSFDKRTD--MEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEE----F 1100
+I + K K+ E++ H+ SL+KE R F E D +N E+E+
Sbjct: 1091 HIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEE-----TIPDHENLSEEEKNILYE 1150
BLAST of MC04g1001 vs. NCBI nr
Match:
XP_022146462.1 (probable RNA-dependent RNA polymerase 1 [Momordica charantia])
HSP 1 Score: 2234 bits (5788), Expect = 0.0
Identity = 1110/1111 (99.91%), Postives = 1110/1111 (99.91%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA
Sbjct: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
Query: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK 120
GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK
Sbjct: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK 120
Query: 121 RENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAPRIFK 180
ENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAPRIFK
Sbjct: 121 GENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAPRIFK 180
Query: 181 KSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF 240
KSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF
Sbjct: 181 KSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF 240
Query: 241 FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQHGYLPGP 300
FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQHGYLPGP
Sbjct: 241 FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQHGYLPGP 300
Query: 301 LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPWKPDI 360
LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPWKPDI
Sbjct: 301 LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPWKPDI 360
Query: 361 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSID 420
SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSID
Sbjct: 361 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSID 420
Query: 421 LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA 480
LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA
Sbjct: 421 LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA 480
Query: 481 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD 540
ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD
Sbjct: 481 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD 540
Query: 541 GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLKYKSL 600
GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLKYKSL
Sbjct: 541 GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLKYKSL 600
Query: 601 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVL 660
DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVL
Sbjct: 601 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVL 660
Query: 661 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 720
ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL
Sbjct: 661 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 720
Query: 721 DETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV 780
DETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV
Sbjct: 721 DETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV 780
Query: 781 DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTI 840
DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTI
Sbjct: 781 DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTI 840
Query: 841 EEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN 900
EEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN
Sbjct: 841 EEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN 900
Query: 901 LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD 960
LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD
Sbjct: 901 LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD 960
Query: 961 MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI 1020
MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI
Sbjct: 961 MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI 1020
Query: 1021 SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGME 1080
SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGME
Sbjct: 1021 SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGME 1080
Query: 1081 RDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
RDHFLSFPWCVSDKLIQIKKKKICQENSSPI
Sbjct: 1081 RDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
BLAST of MC04g1001 vs. NCBI nr
Match:
XP_038875555.1 (probable RNA-dependent RNA polymerase 1 [Benincasa hispida])
HSP 1 Score: 1781 bits (4614), Expect = 0.0
Identity = 885/1116 (79.30%), Postives = 990/1116 (88.71%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
MGKTIEIYGFR +VT +EVKEFLENHTGDGTV VRI++ +DE +RFTF TV+F SELAA
Sbjct: 1 MGKTIEIYGFRPEVTVDEVKEFLENHTGDGTVLTVRISKPKDEKTRFTFVTVRFKSELAA 60
Query: 61 GHVVSQA-AAEERLWYGSSYLKAREADRQN-RRGGVGVERMENLTLHLGSQISKEKLSVI 120
++V +A AAE++LW+GSSYLKARE +R + RGG +ERME++ HLGS ISK+K+ VI
Sbjct: 61 EYIVEKATAAEKKLWFGSSYLKAREVERASPARGGGEMERMEDVKGHLGSLISKDKMRVI 120
Query: 121 WKRENCSVEFGSGVRKLFVYVN--LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAP 180
W+ EN +VEFG GVRKL Y++ +EYKME+ FE+IL V+ RCP + SKF LIQLQ AP
Sbjct: 121 WEGENWNVEFGIGVRKLSFYLSYEVEYKMELAFENILGVEFRCPLNQPSKFFLIQLQGAP 180
Query: 181 RIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ 240
RIFKKS SSSSS +S+ F F RWIRDVDFTPSSCIGQSFALCLELSPG
Sbjct: 181 RIFKKSRSSSSSRLNSN--EFCF--------RWIRDVDFTPSSCIGQSFALCLELSPGHH 240
Query: 241 LPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQHGY 300
LP FF+ LVGY+E Y PF L+TGSSFSS SNLVPI+TPP+GFD S+K LFKINALVQHGY
Sbjct: 241 LPPFFQNLVGYKETYAPFILQTGSSFSSISNLVPIITPPRGFDISYKTLFKINALVQHGY 300
Query: 301 LPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPW 360
LPGP LDDEFFRLVD SRFRP+Y+E ALEK+FNLKEC YEPQKWLK YLS+Y S QLPW
Sbjct: 301 LPGPTLDDEFFRLVDSSRFRPDYVEHALEKMFNLKECCYEPQKWLKHHYLSFYTSNQLPW 360
Query: 361 KPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKI 420
KP+ISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVR++ DDIDNF+RVSFVDE+LDK+
Sbjct: 361 KPNISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEDLDKL 420
Query: 421 RSIDLSPRTSTA-NGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASR 480
SIDL+PR+S+A N ERTRVYDRILS LRNGIVIGDKKFEFLAFSASQLRENS WMFASR
Sbjct: 421 HSIDLAPRSSSAGNSERTRVYDRILSVLRNGIVIGDKKFEFLAFSASQLRENSFWMFASR 480
Query: 481 EGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVT 540
EGLSAADIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEEHE EVI DVEVE
Sbjct: 481 EGLSAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEEHEFEVIPDVEVERKKTK 540
Query: 541 YCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSML 600
YCFSDGIGKIS+TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDPT +KKLSLRKSML
Sbjct: 541 YCFSDGIGKISKTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTLKKKLSLRKSML 600
Query: 601 KYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSR 660
KY SLDTQLDVL WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QL+ IL+DPS+
Sbjct: 601 KYMSLDTQLDVLLWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSK 660
Query: 661 ALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRT 720
ALEVLELMSPGEMT +LKE+LL+YKPN+EPFLNMMLRTFRADK LDLRTKSRIFVPKGRT
Sbjct: 661 ALEVLELMSPGEMTGILKELLLFYKPNEEPFLNMMLRTFRADKFLDLRTKSRIFVPKGRT 720
Query: 721 MMGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKA 780
MMGCLDET TL+YGQVFV CS+PGRS+ESNF+ +GKVVVAKNPCLHPGD+RVL+AVDVKA
Sbjct: 721 MMGCLDETRTLEYGQVFVHCSIPGRSNESNFVIKGKVVVAKNPCLHPGDVRVLDAVDVKA 780
Query: 781 LHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLD 840
LHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFVCWDPEL CI+PVKPMSY+PAPT+QLD
Sbjct: 781 LHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIKPVKPMSYEPAPTMQLD 840
Query: 841 HDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKT 900
HDVTIEEVQEY+ANYMVNDGLGAIANAHTVFAD+ P+KAMS +CIKLAKLFSIAVDFPKT
Sbjct: 841 HDVTIEEVQEYLANYMVNDGLGAIANAHTVFADKNPKKAMSTECIKLAKLFSIAVDFPKT 900
Query: 901 GVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAK 960
GVPANLP LRVHE+PDFM+K ++P Y S GV+GKLF+ VKDVS + NT+E +TREVA
Sbjct: 901 GVPANLPRNLRVHEYPDFMDKPNKPTYASNGVLGKLFQGVKDVSSDVNTLEIFTREVAIN 960
Query: 961 CYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRT 1020
CYDPDME+DGF+KYL+EAFD+KTRYD+KLGNLMDYYGI+TEPELVSGNILR+ KSFDKR
Sbjct: 961 CYDPDMEVDGFDKYLSEAFDYKTRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRN 1020
Query: 1021 DMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRY 1080
D+EQI+ AM+SLRKEARAWFNEKGSK D+ E+EE+AKASAWYHVTYH YWGRY
Sbjct: 1021 DLEQITFAMKSLRKEARAWFNEKGSKSTYDNSKG--EEEEYAKASAWYHVTYHPDYWGRY 1080
Query: 1081 NEGMERDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
NEGM+RDHFLSFPWCV+DKLIQIK++K +SSP+
Sbjct: 1081 NEGMQRDHFLSFPWCVADKLIQIKREKTLLMSSSPM 1104
BLAST of MC04g1001 vs. NCBI nr
Match:
XP_022994004.1 (probable RNA-dependent RNA polymerase 1 [Cucurbita maxima])
HSP 1 Score: 1773 bits (4591), Expect = 0.0
Identity = 882/1122 (78.61%), Postives = 985/1122 (87.79%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
MGK IEIYGF +VTA+EVKEF+ENHTGDGTV VRI++ DE +RFTFATV+F+S+L
Sbjct: 7 MGKMIEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKPNDEKARFTFATVRFTSKLGG 66
Query: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGV--------GVERMENLTLHLGSQISK 120
+VV+QAAAE+RLW+GSSYLKAR+ +R+ R G+ERMEN+ + GS +SK
Sbjct: 67 EYVVAQAAAEKRLWFGSSYLKARKVEREIRTATAAAAARVHGGLERMENVNVQWGSLVSK 126
Query: 121 EKLSVIWKRENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLL 180
EK+ VIWK SVE+G G RKL Y++ +EYKME+ FE+IL VQLRC D S+ L
Sbjct: 127 EKMKVIWKEGKWSVEYGIGARKLGFYLSYEGVEYKMELCFENILGVQLRCSGDRGSELFL 186
Query: 181 IQLQRAPRIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCL 240
IQLQ APRIFKK+ SSSSS SS +S+G RWIRDVDFTPSSCIGQSFALCL
Sbjct: 187 IQLQGAPRIFKKTPSSSSSRLSSE---------ESTGFRWIRDVDFTPSSCIGQSFALCL 246
Query: 241 ELSPGDQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKIN 300
ELSPGDQLPSFF+TLVGY+E Y PF L GSS SS SNLVPI+TPPQGFD S+KILFKIN
Sbjct: 247 ELSPGDQLPSFFRTLVGYKESYAPFILHPGSSLSSISNLVPIITPPQGFDISYKILFKIN 306
Query: 301 ALVQHGYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYY 360
AL+QHGYLPGP LD+EFFRLVD SRFRPEYIE ALEKLF+ KEC YEPQKWLKQQYLS+Y
Sbjct: 307 ALLQHGYLPGPALDNEFFRLVDSSRFRPEYIEYALEKLFHFKECCYEPQKWLKQQYLSFY 366
Query: 361 NSKQLPWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFV 420
+SKQLP KP++SLDDGLVYVHRVQITP+KVYFRGPEANLSNRVVR + DD+DNF+RVSFV
Sbjct: 367 SSKQLPCKPNVSLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFV 426
Query: 421 DEELDKIRSIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSV 480
DEELDK+ SIDLSPRTS N RTRVYDR+LS LRNGIVIGDKKFEFLAFSASQLRENS
Sbjct: 427 DEELDKLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSC 486
Query: 481 WMFASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEV 540
WMFASR+GLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI DVEV
Sbjct: 487 WMFASRKGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVIPDVEV 546
Query: 541 ETNNVTYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLS 600
ETN +TYCFSDGIGKIS TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDPTS KKLS
Sbjct: 547 ETNKITYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLS 606
Query: 601 LRKSMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCI 660
LRKSMLKY SLDTQ+DVL+WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QL+ I
Sbjct: 607 LRKSMLKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSI 666
Query: 661 LKDPSRALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIF 720
L+DPSRALEVLELMSPGEMTS+LKE+LL YKPN+EPFLNMMLRTFRA KLLDLRTKSRIF
Sbjct: 667 LEDPSRALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIF 726
Query: 721 VPKGRTMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLN 780
VPKGRTMMGCLDET TL+YGQVFV CS+PGRSSESNF+ +GKVVVAKNPCLHPGD+R+L+
Sbjct: 727 VPKGRTMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLD 786
Query: 781 AVDVKALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPA 840
AVDVKALHHMV+CVVFPQKG+RPHPNECSGSDLDGDLYFV WDPEL I+PVKPMSY+PA
Sbjct: 787 AVDVKALHHMVNCVVFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPA 846
Query: 841 PTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIA 900
PT++LDHDVTIEEVQEY+A YMVNDGLG IANAHTVFAD++P+KAM+A+CIKLAKLFSIA
Sbjct: 847 PTMRLDHDVTIEEVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIA 906
Query: 901 VDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYT 960
VDFPKTGVPAN P LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NTIE ++
Sbjct: 907 VDFPKTGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTIEIFS 966
Query: 961 REVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGK 1020
REVA KCYDPDME+DGFE YL+EAF++K RYD+KLGNLMDYYGI+TEPEL+SGNILR+ K
Sbjct: 967 REVATKCYDPDMEVDGFEDYLSEAFEYKARYDFKLGNLMDYYGIKTEPELISGNILRMAK 1026
Query: 1021 SFDKRTDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQ 1080
SFDKR D+EQIS A++SLRKEAR+WFNEKGSK CD+DD DEE+AKASAWY VTYH
Sbjct: 1027 SFDKRNDLEQISLAIKSLRKEARSWFNEKGSKSACDEDDK---DEEYAKASAWYRVTYHP 1086
Query: 1081 SYWGRYNEGMERDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
YWGRYNEGM+RDHFLSFPWCVSDKLIQIK++K C N SPI
Sbjct: 1087 DYWGRYNEGMQRDHFLSFPWCVSDKLIQIKREKTCLVNLSPI 1116
BLAST of MC04g1001 vs. NCBI nr
Match:
XP_022938807.1 (probable RNA-dependent RNA polymerase 1 [Cucurbita moschata])
HSP 1 Score: 1770 bits (4585), Expect = 0.0
Identity = 880/1117 (78.78%), Postives = 985/1117 (88.18%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
MGKTIEIYGF VTA+EVKEF+ENHTGDGTV VRI++ DE +RFTFATVQF+S+L
Sbjct: 7 MGKTIEIYGFPTQVTADEVKEFIENHTGDGTVLTVRISKLNDEKARFTFATVQFTSKLGG 66
Query: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNR---RGGVGVERMENLTLHLGSQISKEKLSV 120
+VV+QAAAE+RLW+GSSYLKAR+ +R+ + R +ERMEN+ + GS +SKE++ V
Sbjct: 67 EYVVAQAAAEKRLWFGSSYLKARKVEREIKTAARVHGELERMENVNVQWGSLVSKEEMKV 126
Query: 121 IWKRENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQR 180
IWK SVE+G GVRKL Y++ +EYKME+ FE+IL VQLRC D SK LIQLQ
Sbjct: 127 IWKGGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLFLIQLQG 186
Query: 181 APRIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPG 240
APRIFKK+ SSSS SS +S+G RWIRDVDFTPSSCIGQSFALCLELS G
Sbjct: 187 APRIFKKAPLSSSSRLSSE---------ESTGFRWIRDVDFTPSSCIGQSFALCLELSHG 246
Query: 241 DQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQH 300
DQLPSFF+TLVGY+E Y PF L+TGSS SS SNLVPI+TPPQGFD +KILFKINAL+QH
Sbjct: 247 DQLPSFFQTLVGYKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALLQH 306
Query: 301 GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQL 360
GYLPGP LD+EFFRLVD SRFRP+Y+E AL+KLF+ KEC YEPQKWLKQQYLS+Y+SKQL
Sbjct: 307 GYLPGPALDNEFFRLVDSSRFRPDYVEYALDKLFHFKECCYEPQKWLKQQYLSFYSSKQL 366
Query: 361 PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 420
PWKP+ISLDDGLVYVHRVQITP+KVYFRGPEANLSNRVVR + DD+DNF+RVSFVDEELD
Sbjct: 367 PWKPNISLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELD 426
Query: 421 KIRSIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 480
K+ SIDLSPRTS N RTRVYDR+LS LRNGIVIGDKKFEFLAFSASQLRENS WMFAS
Sbjct: 427 KLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFAS 486
Query: 481 REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 540
R+GLSAADIREWMGD RQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI+DVEVETN +
Sbjct: 487 RKGLSAADIREWMGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETNKI 546
Query: 541 TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSM 600
TYCFSDGIGKIS TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDPTS KKLSLRKSM
Sbjct: 547 TYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSM 606
Query: 601 LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPS 660
LKY SLDTQ+DVL+WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QL+ IL+DPS
Sbjct: 607 LKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPS 666
Query: 661 RALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGR 720
RALEVLELMSPGEMTS+LKE+LL YKPN+EPFLNMMLRTFRA KLLDLRTKSRIFVPKGR
Sbjct: 667 RALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGR 726
Query: 721 TMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVK 780
TMMGCLDET TL+YGQVFV CS+PGRSSESNF+ +GKVVVAKNPCLHPGD+R+L+AVDVK
Sbjct: 727 TMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVK 786
Query: 781 ALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQL 840
ALHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFV WDPEL I+PVKPMSY+PAPT+QL
Sbjct: 787 ALHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMQL 846
Query: 841 DHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPK 900
DHDVTIEEVQ Y+ YMVNDGLG IANAHTVFAD++P+KAM+A+CIKLAKLFSIAVDFPK
Sbjct: 847 DHDVTIEEVQGYLVKYMVNDGLGGIANAHTVFADKKPKKAMAAECIKLAKLFSIAVDFPK 906
Query: 901 TGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAA 960
TGVPAN P LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NT+E ++REVA
Sbjct: 907 TGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREVAT 966
Query: 961 KCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKR 1020
KCYDPDME+DGFE YL+EAF+FK RYD+KLGNLMDYYGI+TEPEL+SGNILR+ KSFDKR
Sbjct: 967 KCYDPDMEVDGFEDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKR 1026
Query: 1021 TDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGR 1080
DMEQIS AM+SLRKEAR+WFNEKGSK D+DDN ++EE+AKASAWY VTYH YWGR
Sbjct: 1027 NDMEQISLAMKSLRKEARSWFNEKGSKSAYDEDDN--KNEEYAKASAWYRVTYHPDYWGR 1086
Query: 1081 YNEGMERDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
YNEGM+RDHFLSFPWCVSDKLIQIK++K C N SP+
Sbjct: 1087 YNEGMQRDHFLSFPWCVSDKLIQIKREKTCLVNFSPV 1112
BLAST of MC04g1001 vs. NCBI nr
Match:
KAG6579193.1 (RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1769 bits (4582), Expect = 0.0
Identity = 877/1112 (78.87%), Postives = 983/1112 (88.40%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
MGKTIEIYGF +VTA+EVKEF+ENHTGDGTV VRI++F DE +RFTFATV+F+S+L
Sbjct: 7 MGKTIEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKFNDEKARFTFATVRFTSKLGG 66
Query: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGVG-----VERMENLTLHLGSQISKEKL 120
+VV+QAAAE+RLW+GSSYLKAR+ +R+ + +ERMEN+ + GS +SKEK+
Sbjct: 67 EYVVAQAAAEKRLWFGSSYLKARKVEREIKTAAAARVHGELERMENVNVQWGSLVSKEKM 126
Query: 121 SVIWKRENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLLIQL 180
VIWK SVE+G GVRKL Y++ +EYKME+ FE+IL VQLRC D SK LIQL
Sbjct: 127 KVIWKGGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLFLIQL 186
Query: 181 QRAPRIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELS 240
Q APRIFKK+ SSSS SS +S+G RWIRDVDFTPSSCIGQSFALCLELS
Sbjct: 187 QGAPRIFKKAPLSSSSRLSSE---------ESTGFRWIRDVDFTPSSCIGQSFALCLELS 246
Query: 241 PGDQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALV 300
GDQLPSFF+TLVGY+E Y PF L+TGSS SS SNLVPI+TPPQGFD +KILFKINAL+
Sbjct: 247 HGDQLPSFFQTLVGYKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALL 306
Query: 301 QHGYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSK 360
QHGYLPGP LD+EFFRLVD SRFRP+Y+E AL+KLF+LKEC YEPQKWLKQQYLS+Y+SK
Sbjct: 307 QHGYLPGPALDNEFFRLVDSSRFRPDYVEYALDKLFHLKECCYEPQKWLKQQYLSFYSSK 366
Query: 361 QLPWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEE 420
QLPWKP+ISLDDGLVYVHRVQITP+KVYFRGPEANLSNRVVR + DD+DNF+RVSFVDEE
Sbjct: 367 QLPWKPNISLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEE 426
Query: 421 LDKIRSIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMF 480
LDK+ SIDLSPRTS N RTRVYDR+LS LRNGIVIGDKKFEFLAFSASQLRENS WMF
Sbjct: 427 LDKLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMF 486
Query: 481 ASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETN 540
ASR+GLSAADIREWMGD RQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI+DVEVETN
Sbjct: 487 ASRKGLSAADIREWMGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETN 546
Query: 541 NVTYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRK 600
+TYCFSDGIGKIS TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDPTS KKLSLRK
Sbjct: 547 KITYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRK 606
Query: 601 SMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKD 660
SMLKY SLDTQ+DVL+WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QL+ IL+D
Sbjct: 607 SMLKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILED 666
Query: 661 PSRALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPK 720
PSRALEVLELMSPGEMTS+LKE+LL YKPN+EPFLNMMLRTFRA KLLDLRTKSRIFVPK
Sbjct: 667 PSRALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPK 726
Query: 721 GRTMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVD 780
GRTMMGCLDET TL+YGQVFV CS+PGRSSESNF+ +GKVVVAKNPCLHPGD+R+L+AVD
Sbjct: 727 GRTMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVD 786
Query: 781 VKALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTI 840
VKALHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFV WDPEL I+PVKPMSY+PAPT+
Sbjct: 787 VKALHHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTM 846
Query: 841 QLDHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDF 900
QLDHDVTIEEVQ Y+ YMVNDGLG IANAHTVFAD++P+KAM+A+CIKLAKLFSIAVDF
Sbjct: 847 QLDHDVTIEEVQGYLVKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIAVDF 906
Query: 901 PKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREV 960
PKTGVPAN P LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NT+E ++REV
Sbjct: 907 PKTGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREV 966
Query: 961 AAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFD 1020
A KCYDPDME+DGFE YL+EAF+FK RYD+KLGNLMDYYGI+TEPEL+SGNILR+ KSFD
Sbjct: 967 ATKCYDPDMEVDGFEDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFD 1026
Query: 1021 KRTDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYW 1080
KR DMEQIS AM+SLRKEAR+WFNEKGSK D+DDN ++EE+AKASAWY VTYH YW
Sbjct: 1027 KRNDMEQISLAMKSLRKEARSWFNEKGSKSAYDEDDN--KNEEYAKASAWYRVTYHPDYW 1086
Query: 1081 GRYNEGMERDHFLSFPWCVSDKLIQIKKKKIC 1104
GRYNEGM+RDHFLSFPWCVSDKLI+IK++K C
Sbjct: 1087 GRYNEGMQRDHFLSFPWCVSDKLIRIKREKTC 1107
BLAST of MC04g1001 vs. ExPASy TrEMBL
Match:
A0A6J1CZM7 (RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015673 PE=3 SV=1)
HSP 1 Score: 2234 bits (5788), Expect = 0.0
Identity = 1110/1111 (99.91%), Postives = 1110/1111 (99.91%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA
Sbjct: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
Query: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK 120
GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK
Sbjct: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK 120
Query: 121 RENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAPRIFK 180
ENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAPRIFK
Sbjct: 121 GENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAPRIFK 180
Query: 181 KSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF 240
KSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF
Sbjct: 181 KSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF 240
Query: 241 FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQHGYLPGP 300
FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQHGYLPGP
Sbjct: 241 FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQHGYLPGP 300
Query: 301 LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPWKPDI 360
LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPWKPDI
Sbjct: 301 LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPWKPDI 360
Query: 361 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSID 420
SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSID
Sbjct: 361 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSID 420
Query: 421 LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA 480
LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA
Sbjct: 421 LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA 480
Query: 481 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD 540
ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD
Sbjct: 481 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD 540
Query: 541 GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLKYKSL 600
GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLKYKSL
Sbjct: 541 GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLKYKSL 600
Query: 601 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVL 660
DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVL
Sbjct: 601 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVL 660
Query: 661 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 720
ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL
Sbjct: 661 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 720
Query: 721 DETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV 780
DETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV
Sbjct: 721 DETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV 780
Query: 781 DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTI 840
DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTI
Sbjct: 781 DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTI 840
Query: 841 EEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN 900
EEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN
Sbjct: 841 EEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN 900
Query: 901 LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD 960
LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD
Sbjct: 901 LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD 960
Query: 961 MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI 1020
MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI
Sbjct: 961 MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI 1020
Query: 1021 SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGME 1080
SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGME
Sbjct: 1021 SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGME 1080
Query: 1081 RDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
RDHFLSFPWCVSDKLIQIKKKKICQENSSPI
Sbjct: 1081 RDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
BLAST of MC04g1001 vs. ExPASy TrEMBL
Match:
A0A6J1K1Q3 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111489826 PE=3 SV=1)
HSP 1 Score: 1773 bits (4591), Expect = 0.0
Identity = 882/1122 (78.61%), Postives = 985/1122 (87.79%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
MGK IEIYGF +VTA+EVKEF+ENHTGDGTV VRI++ DE +RFTFATV+F+S+L
Sbjct: 7 MGKMIEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKPNDEKARFTFATVRFTSKLGG 66
Query: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGV--------GVERMENLTLHLGSQISK 120
+VV+QAAAE+RLW+GSSYLKAR+ +R+ R G+ERMEN+ + GS +SK
Sbjct: 67 EYVVAQAAAEKRLWFGSSYLKARKVEREIRTATAAAAARVHGGLERMENVNVQWGSLVSK 126
Query: 121 EKLSVIWKRENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLL 180
EK+ VIWK SVE+G G RKL Y++ +EYKME+ FE+IL VQLRC D S+ L
Sbjct: 127 EKMKVIWKEGKWSVEYGIGARKLGFYLSYEGVEYKMELCFENILGVQLRCSGDRGSELFL 186
Query: 181 IQLQRAPRIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCL 240
IQLQ APRIFKK+ SSSSS SS +S+G RWIRDVDFTPSSCIGQSFALCL
Sbjct: 187 IQLQGAPRIFKKTPSSSSSRLSSE---------ESTGFRWIRDVDFTPSSCIGQSFALCL 246
Query: 241 ELSPGDQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKIN 300
ELSPGDQLPSFF+TLVGY+E Y PF L GSS SS SNLVPI+TPPQGFD S+KILFKIN
Sbjct: 247 ELSPGDQLPSFFRTLVGYKESYAPFILHPGSSLSSISNLVPIITPPQGFDISYKILFKIN 306
Query: 301 ALVQHGYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYY 360
AL+QHGYLPGP LD+EFFRLVD SRFRPEYIE ALEKLF+ KEC YEPQKWLKQQYLS+Y
Sbjct: 307 ALLQHGYLPGPALDNEFFRLVDSSRFRPEYIEYALEKLFHFKECCYEPQKWLKQQYLSFY 366
Query: 361 NSKQLPWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFV 420
+SKQLP KP++SLDDGLVYVHRVQITP+KVYFRGPEANLSNRVVR + DD+DNF+RVSFV
Sbjct: 367 SSKQLPCKPNVSLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFV 426
Query: 421 DEELDKIRSIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSV 480
DEELDK+ SIDLSPRTS N RTRVYDR+LS LRNGIVIGDKKFEFLAFSASQLRENS
Sbjct: 427 DEELDKLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSC 486
Query: 481 WMFASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEV 540
WMFASR+GLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI DVEV
Sbjct: 487 WMFASRKGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVIPDVEV 546
Query: 541 ETNNVTYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLS 600
ETN +TYCFSDGIGKIS TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDPTS KKLS
Sbjct: 547 ETNKITYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLS 606
Query: 601 LRKSMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCI 660
LRKSMLKY SLDTQ+DVL+WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QL+ I
Sbjct: 607 LRKSMLKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSI 666
Query: 661 LKDPSRALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIF 720
L+DPSRALEVLELMSPGEMTS+LKE+LL YKPN+EPFLNMMLRTFRA KLLDLRTKSRIF
Sbjct: 667 LEDPSRALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIF 726
Query: 721 VPKGRTMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLN 780
VPKGRTMMGCLDET TL+YGQVFV CS+PGRSSESNF+ +GKVVVAKNPCLHPGD+R+L+
Sbjct: 727 VPKGRTMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLD 786
Query: 781 AVDVKALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPA 840
AVDVKALHHMV+CVVFPQKG+RPHPNECSGSDLDGDLYFV WDPEL I+PVKPMSY+PA
Sbjct: 787 AVDVKALHHMVNCVVFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPA 846
Query: 841 PTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIA 900
PT++LDHDVTIEEVQEY+A YMVNDGLG IANAHTVFAD++P+KAM+A+CIKLAKLFSIA
Sbjct: 847 PTMRLDHDVTIEEVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIA 906
Query: 901 VDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYT 960
VDFPKTGVPAN P LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NTIE ++
Sbjct: 907 VDFPKTGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTIEIFS 966
Query: 961 REVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGK 1020
REVA KCYDPDME+DGFE YL+EAF++K RYD+KLGNLMDYYGI+TEPEL+SGNILR+ K
Sbjct: 967 REVATKCYDPDMEVDGFEDYLSEAFEYKARYDFKLGNLMDYYGIKTEPELISGNILRMAK 1026
Query: 1021 SFDKRTDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQ 1080
SFDKR D+EQIS A++SLRKEAR+WFNEKGSK CD+DD DEE+AKASAWY VTYH
Sbjct: 1027 SFDKRNDLEQISLAIKSLRKEARSWFNEKGSKSACDEDDK---DEEYAKASAWYRVTYHP 1086
Query: 1081 SYWGRYNEGMERDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
YWGRYNEGM+RDHFLSFPWCVSDKLIQIK++K C N SPI
Sbjct: 1087 DYWGRYNEGMQRDHFLSFPWCVSDKLIQIKREKTCLVNLSPI 1116
BLAST of MC04g1001 vs. ExPASy TrEMBL
Match:
A0A6J1FF44 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111444909 PE=3 SV=1)
HSP 1 Score: 1770 bits (4585), Expect = 0.0
Identity = 880/1117 (78.78%), Postives = 985/1117 (88.18%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
MGKTIEIYGF VTA+EVKEF+ENHTGDGTV VRI++ DE +RFTFATVQF+S+L
Sbjct: 7 MGKTIEIYGFPTQVTADEVKEFIENHTGDGTVLTVRISKLNDEKARFTFATVQFTSKLGG 66
Query: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNR---RGGVGVERMENLTLHLGSQISKEKLSV 120
+VV+QAAAE+RLW+GSSYLKAR+ +R+ + R +ERMEN+ + GS +SKE++ V
Sbjct: 67 EYVVAQAAAEKRLWFGSSYLKARKVEREIKTAARVHGELERMENVNVQWGSLVSKEEMKV 126
Query: 121 IWKRENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQR 180
IWK SVE+G GVRKL Y++ +EYKME+ FE+IL VQLRC D SK LIQLQ
Sbjct: 127 IWKGGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLFLIQLQG 186
Query: 181 APRIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPG 240
APRIFKK+ SSSS SS +S+G RWIRDVDFTPSSCIGQSFALCLELS G
Sbjct: 187 APRIFKKAPLSSSSRLSSE---------ESTGFRWIRDVDFTPSSCIGQSFALCLELSHG 246
Query: 241 DQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQH 300
DQLPSFF+TLVGY+E Y PF L+TGSS SS SNLVPI+TPPQGFD +KILFKINAL+QH
Sbjct: 247 DQLPSFFQTLVGYKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALLQH 306
Query: 301 GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQL 360
GYLPGP LD+EFFRLVD SRFRP+Y+E AL+KLF+ KEC YEPQKWLKQQYLS+Y+SKQL
Sbjct: 307 GYLPGPALDNEFFRLVDSSRFRPDYVEYALDKLFHFKECCYEPQKWLKQQYLSFYSSKQL 366
Query: 361 PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 420
PWKP+ISLDDGLVYVHRVQITP+KVYFRGPEANLSNRVVR + DD+DNF+RVSFVDEELD
Sbjct: 367 PWKPNISLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELD 426
Query: 421 KIRSIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 480
K+ SIDLSPRTS N RTRVYDR+LS LRNGIVIGDKKFEFLAFSASQLRENS WMFAS
Sbjct: 427 KLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFAS 486
Query: 481 REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 540
R+GLSAADIREWMGD RQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI+DVEVETN +
Sbjct: 487 RKGLSAADIREWMGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETNKI 546
Query: 541 TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSM 600
TYCFSDGIGKIS TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDPTS KKLSLRKSM
Sbjct: 547 TYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSM 606
Query: 601 LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPS 660
LKY SLDTQ+DVL+WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QL+ IL+DPS
Sbjct: 607 LKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPS 666
Query: 661 RALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGR 720
RALEVLELMSPGEMTS+LKE+LL YKPN+EPFLNMMLRTFRA KLLDLRTKSRIFVPKGR
Sbjct: 667 RALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGR 726
Query: 721 TMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVK 780
TMMGCLDET TL+YGQVFV CS+PGRSSESNF+ +GKVVVAKNPCLHPGD+R+L+AVDVK
Sbjct: 727 TMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVK 786
Query: 781 ALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQL 840
ALHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFV WDPEL I+PVKPMSY+PAPT+QL
Sbjct: 787 ALHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMQL 846
Query: 841 DHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPK 900
DHDVTIEEVQ Y+ YMVNDGLG IANAHTVFAD++P+KAM+A+CIKLAKLFSIAVDFPK
Sbjct: 847 DHDVTIEEVQGYLVKYMVNDGLGGIANAHTVFADKKPKKAMAAECIKLAKLFSIAVDFPK 906
Query: 901 TGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAA 960
TGVPAN P LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NT+E ++REVA
Sbjct: 907 TGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREVAT 966
Query: 961 KCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKR 1020
KCYDPDME+DGFE YL+EAF+FK RYD+KLGNLMDYYGI+TEPEL+SGNILR+ KSFDKR
Sbjct: 967 KCYDPDMEVDGFEDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKR 1026
Query: 1021 TDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGR 1080
DMEQIS AM+SLRKEAR+WFNEKGSK D+DDN ++EE+AKASAWY VTYH YWGR
Sbjct: 1027 NDMEQISLAMKSLRKEARSWFNEKGSKSAYDEDDN--KNEEYAKASAWYRVTYHPDYWGR 1086
Query: 1081 YNEGMERDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
YNEGM+RDHFLSFPWCVSDKLIQIK++K C N SP+
Sbjct: 1087 YNEGMQRDHFLSFPWCVSDKLIQIKREKTCLVNFSPV 1112
BLAST of MC04g1001 vs. ExPASy TrEMBL
Match:
A0A6J1JTB1 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111487656 PE=3 SV=1)
HSP 1 Score: 1746 bits (4521), Expect = 0.0
Identity = 874/1115 (78.39%), Postives = 979/1115 (87.80%), Query Frame = 0
Query: 2 GKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAAG 61
GKTIE++GFRR+V+A EVKEF+ENHTG+GTVS VRI++ +D R TFA V+F S++AA
Sbjct: 5 GKTIEVFGFRREVSAGEVKEFMENHTGEGTVSKVRISKPKDGKGRLTFARVEFRSKVAAE 64
Query: 62 HVVSQAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWKR 121
+VV +A AEE LW+G YLKAR +R+ R VERME + + LGS ISKE++ VIW+
Sbjct: 65 YVVEKAEAEEGLWFGRWYLKARGVEREMR-----VERMEKVRVQLGSVISKEEMRVIWRG 124
Query: 122 ENCSVEFGSGVRKLFVYVNL-----EYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAP 181
E SVE+G+G+RKL+ Y++ +YKME+ FE+IL VQLRCP + +S LIQLQ AP
Sbjct: 125 EEWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQLQGAP 184
Query: 182 RIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ 241
RIFKK SSSSSSS +FY KES +G RWIRDVDFTPSSCIGQSF+LCLE+SPGDQ
Sbjct: 185 RIFKKCPSSSSSSS-----QFYSKES--TGFRWIRDVDFTPSSCIGQSFSLCLEVSPGDQ 244
Query: 242 LPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQHGY 301
LPSFF+TLVGY+EIYGPF L TGS+FSSTSNLVPIVTPPQ FD +KILFKINALVQHGY
Sbjct: 245 LPSFFQTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFDIPYKILFKINALVQHGY 304
Query: 302 LPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPW 361
LPG LDD+FFRLVDPSRFR +YIE ALEKLF+LKEC YEPQKWLKQQYLS+ +S QLPW
Sbjct: 305 LPGSALDDKFFRLVDPSRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPW 364
Query: 362 KPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKI 421
K +ISLDD LVYVHRVQITP+KVYFRGPEANLSNRVVR++ DDIDNF+RVSFVDEELDKI
Sbjct: 365 KANISLDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKI 424
Query: 422 RSIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASRE 481
S DLSPRTST ERTRVYDRILS LRNGIVIG KKFEFLAFSASQLRENS WMFASRE
Sbjct: 425 HSSDLSPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASRE 484
Query: 482 GLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTY 541
GL+AADIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVI D EVE N+ Y
Sbjct: 485 GLNAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDAEVERKNIKY 544
Query: 542 CFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLK 601
CFSDGIGKISE LAE VAE+CGLI+HTPSAFQIRYAGYKGVVA+DPTS+KKLSLRKSMLK
Sbjct: 545 CFSDGIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLK 604
Query: 602 YKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRA 661
Y SLDTQLDVL+WSKY PCFLNRQVINLLSTLGI+D VF K+QK+A+ QL+ IL+DPS+A
Sbjct: 605 YMSLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIRDDVFVKQQKKAVDQLDSILRDPSKA 664
Query: 662 LEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTM 721
LEVLELMSPGEMTSVLK+MLL+Y P++EPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTM
Sbjct: 665 LEVLELMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTM 724
Query: 722 MGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKAL 781
MGCLDET TL+YGQVF+ CSVPGRSSESNF+ +GKVVVAKNPCLHPGD+RVL+AVDVK L
Sbjct: 725 MGCLDETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTL 784
Query: 782 HHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDH 841
HHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFVCWDPEL CIRP+KPMSY+PAPTIQLDH
Sbjct: 785 HHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDH 844
Query: 842 DVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTG 901
DVT+EEVQEY ANYMVNDGLG IANAHTVFAD +P+KAMSA+CI+LAKLFSIAVDFPKTG
Sbjct: 845 DVTLEEVQEYFANYMVNDGLGGIANAHTVFADMKPKKAMSAECIELAKLFSIAVDFPKTG 904
Query: 902 VPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKC 961
VPANLP LRVHE+PDFMEK ++ Y S GVIGKLFR VKDVS + N IET+TREVA KC
Sbjct: 905 VPANLPRSLRVHEYPDFMEKPNKRTYISNGVIGKLFRGVKDVSSDINNIETFTREVAVKC 964
Query: 962 YDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTD 1021
YDPDME+ GFE YL+EAFD+K YD+KLGNLMDYYGI TEPELVSGNILR+GKSFDKR D
Sbjct: 965 YDPDMEVMGFENYLSEAFDYKRMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRND 1024
Query: 1022 MEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYN 1081
MEQIS AMRSLRKEAR WFNEK SK ++ N ED E+AKASAWYHVTYH ++WGRYN
Sbjct: 1025 MEQISLAMRSLRKEARGWFNEKASKSIYNN--NKGEDNEYAKASAWYHVTYHPNFWGRYN 1084
Query: 1082 EGMERDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
EGM+RDHFLSFPWCVSDKLIQIK++K+ SSP+
Sbjct: 1085 EGMQRDHFLSFPWCVSDKLIQIKREKM---ESSPV 1102
BLAST of MC04g1001 vs. ExPASy TrEMBL
Match:
A0A6J1EB47 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111430971 PE=3 SV=1)
HSP 1 Score: 1742 bits (4512), Expect = 0.0
Identity = 872/1117 (78.07%), Postives = 985/1117 (88.18%), Query Frame = 0
Query: 2 GKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAAG 61
GKTIE++GF R+V+A EVKEF+ENHTG+GTVS VRI++ + E SR T A V+F S++AA
Sbjct: 5 GKTIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISKPKYEKSRLTSARVEFRSKVAAE 64
Query: 62 HVVSQAAAEERLWYGSSYLKAREADRQNRRGG--VGVERMENLTLHLGSQISKEKLSVIW 121
+VV +A AEERLW+G YLKAR +R+ R +ER+E + + LGS I K+++ VIW
Sbjct: 65 YVVEKAEAEERLWFGRWYLKARRVEREREREMRVERLERLEKVRVQLGSVIWKDEMRVIW 124
Query: 122 KRENCSVEFGSGVRKLFVYVNL-----EYKMEVWFESILRVQLRCPPDGASKFLLIQLQR 181
+ E+ SVE+G+G+RKL+ Y++ +YKME+ FE+IL VQLRCP + +S LIQLQ
Sbjct: 125 RGEDWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQLQG 184
Query: 182 APRIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPG 241
APRIFKK SSSSSSSS +FY KES +G RWIRDVDFTPSSCIGQSFALC E+SPG
Sbjct: 185 APRIFKKCPSSSSSSSS----QFYSKES--TGFRWIRDVDFTPSSCIGQSFALCFEVSPG 244
Query: 242 DQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQH 301
DQLPSFF+TLVGY+EIYGPF L TGS+FSSTSNLVPIVTPPQ F +KILFKINALVQH
Sbjct: 245 DQLPSFFQTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFHIPYKILFKINALVQH 304
Query: 302 GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQL 361
GYLPG LDD+FFRLVDP+RFR +YIE ALEKLF+LKEC YEPQKWLKQQYLS+ +S QL
Sbjct: 305 GYLPGSALDDKFFRLVDPTRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQL 364
Query: 362 PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 421
PWK +ISLDD LVYVHRVQITP+KVYFRGPEANLSNRVVR++ DDIDNF+RVSFVDEELD
Sbjct: 365 PWKANISLDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELD 424
Query: 422 KIRSIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 481
KI S DLSPRTST ERTRVYDRILS LRNGIVIG KKFEFLAFSASQLRENS WMFAS
Sbjct: 425 KIYSSDLSPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFAS 484
Query: 482 REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 541
REGL+AADIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVI DVEVE N+
Sbjct: 485 REGLNAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNI 544
Query: 542 TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSM 601
YCFSDGIGKISE LAE VAE+CGLI+HTPSAFQIRYAGYKGVVA+DPTS+KKLSLRKSM
Sbjct: 545 KYCFSDGIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSM 604
Query: 602 LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPS 661
LKY SLDTQLDVL+WSKY PCFLNRQVINLLSTLGI+D VF K+QK+A+ QL+ IL+DPS
Sbjct: 605 LKYMSLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIQDDVFVKQQKKAVDQLDSILRDPS 664
Query: 662 RALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGR 721
+ALEVLELMSPGEMTSVLK+MLL+Y P++EPFLNMMLRTFRADKLLDLRTKSRIFVP+GR
Sbjct: 665 KALEVLELMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPEGR 724
Query: 722 TMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVK 781
TMMGCLDET TL+YGQVF+ CSVPGRSSESNF+ +GKVVVAKNPCLHPGD+RVL+AVDVK
Sbjct: 725 TMMGCLDETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVK 784
Query: 782 ALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQL 841
LHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFVCWDPEL CIRP+KPMSY+PAPTIQL
Sbjct: 785 TLHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQL 844
Query: 842 DHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPK 901
DHDVTIEEVQEY ANYMVNDGLG IANAHTVFAD++P+KAMSA+CI+LAKLFSIAVDFPK
Sbjct: 845 DHDVTIEEVQEYFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPK 904
Query: 902 TGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAA 961
TGVPANLP LRVHE+PDFMEK ++ Y S GVIGKLFR VKDVS + N IET+TREVAA
Sbjct: 905 TGVPANLPRSLRVHEYPDFMEKPNKRTYTSNGVIGKLFRGVKDVSSDINNIETFTREVAA 964
Query: 962 KCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKR 1021
KCYDPDME+ GFE YL+EAFD+K+ YD+KLGNLMDYYGI TEPELVSGNILR+GKSFDKR
Sbjct: 965 KCYDPDMEVMGFENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKR 1024
Query: 1022 TDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGR 1081
DMEQIS AMRSLRKEAR WFNEKGSK ++ N DED+E+AKASAWYHVTYH ++WGR
Sbjct: 1025 NDMEQISLAMRSLRKEARGWFNEKGSKSIYNN--NKDEDDEYAKASAWYHVTYHPNFWGR 1084
Query: 1082 YNEGMERDHFLSFPWCVSDKLIQIKKKKICQENSSPI 1111
YNEGM+RDHFLSFPWCVS+KLIQIK++K+ SSP+
Sbjct: 1085 YNEGMQRDHFLSFPWCVSEKLIQIKREKM---ESSPV 1110
BLAST of MC04g1001 vs. TAIR 10
Match:
AT1G14790.1 (RNA-dependent RNA polymerase 1 )
HSP 1 Score: 1230.7 bits (3183), Expect = 0.0e+00
Identity = 643/1110 (57.93%), Postives = 802/1110 (72.25%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
MGKTI+++GF V+AEEVK+FLE TG GTV A+++ + + R +A VQF+SE
Sbjct: 1 MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPR-VYAIVQFTSERHT 60
Query: 61 GHVVSQAAAEERLWYGSSYLKAREADRQ-NRRGGVGVERMENLTLHLGSQISKEKLSVIW 120
+++ AA ERL+YG SYLKA E ++ + + + L + G Q+S +K +W
Sbjct: 61 RLIIT--AAAERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLW 120
Query: 121 KRENCSVEFGSGVRKL---FVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAP 180
++ V FG G+RKL F + +Y++E+ +E+I ++ L P +SKFL+IQ+ AP
Sbjct: 121 SAQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIGAP 180
Query: 181 RIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ 240
+IF+K + FY S S +WIR DFT SSCIGQS A CLEL
Sbjct: 181 KIFEKEDQPINLLFGIMD--FY---SDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLN 240
Query: 241 LPSFFKTLVGY-EEIYGPFNLETGSSFSSTSN-LVPIVTPPQGFDFSFKILFKINALVQH 300
+P F + Y E F +E+GSS+SS +N LVP+V PP GF F+ILFK+N LVQ+
Sbjct: 241 VPDFRENFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQN 300
Query: 301 GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQL 360
L GP LD +F+RL++ ++ I+ LEKLF+L EC YEP WL+ +Y + + +L
Sbjct: 301 ACLSGPALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKL 360
Query: 361 PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 420
P P ISLDDGLVY++RVQ+TP++VYF GPE N+SNRV+R YS I+NF+RVSFVDE+L+
Sbjct: 361 PLSPTISLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLE 420
Query: 421 KIRSIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 480
K+RS+DLSPR+ST RT++YDRI S LR+GIVIGDKKFEFLAFS+SQLRENS WMFA
Sbjct: 421 KVRSMDLSPRSSTQ--RRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAP 480
Query: 481 REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 540
+ ++AA IR WMGDF IRNVAKYAARLGQSF SSR+TL V EIEVI DVE+ +
Sbjct: 481 IDRITAAHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGT 540
Query: 541 TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSM 600
Y FSDGIGKIS A KVA +CGL +PSAFQIRY GYKGVVA+DP S KKLSLRKSM
Sbjct: 541 RYVFSDGIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSM 600
Query: 601 LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPS 660
K++S +T+LDVLAWSKY PC++NRQ+I LLSTLG+ D VFEKKQ+E + +L+ IL P
Sbjct: 601 SKFESENTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPL 660
Query: 661 RALEVLELMSPGEMTSVLKEMLLY-YKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKG 720
A E L LM+PGE T++LK ++L YKP+ EPFL+MML+ FRA KLL+LRTK+RIF+ G
Sbjct: 661 EAHEALGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGG 720
Query: 721 RTMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDV 780
R+MMGCLDET TL+YGQV VQ S P R FI G VVVAKNPCLHPGD+RVL AV+V
Sbjct: 721 RSMMGCLDETRTLEYGQVVVQYSDPMRPGR-RFIITGPVVVAKNPCLHPGDVRVLQAVNV 780
Query: 781 KALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQ 840
AL+HMVDCVVFPQKG RPHPNECSGSDLDGD+YFVCWD EL R +PM Y P PT
Sbjct: 781 PALNHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQI 840
Query: 841 LDHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFP 900
LDHDVTIEEV+EY ANY+VND LG IANAHT FAD+EP KA S CI+LAK FS AVDFP
Sbjct: 841 LDHDVTIEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFP 900
Query: 901 KTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVA 960
KTGV A +P L V E+PDFMEK D+P YESK VIGKLFR VK+ + +I+++T +VA
Sbjct: 901 KTGVAAVIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVA 960
Query: 961 AKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDK 1020
+K YD DME+DGFE+Y++EAF K YD+KLGNLMDYYGI+TE E++SG I+R+ KSF K
Sbjct: 961 SKSYDKDMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTK 1020
Query: 1021 RTDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWG 1080
R D E I A+R+LRKE + FN + +E+ E AKASAWYHVTYH SYWG
Sbjct: 1021 RRDAESIGRAVRALRKETLSLFNA-----------SEEEENESAKASAWYHVTYHSSYWG 1080
Query: 1081 RYNEGMERDHFLSFPWCVSDKLIQIKKKKI 1104
YNEG+ RDHFLSF WCV DKL++IKK +
Sbjct: 1081 LYNEGLNRDHFLSFAWCVYDKLVRIKKTNL 1088
BLAST of MC04g1001 vs. TAIR 10
Match:
AT4G11130.1 (RNA-dependent RNA polymerase 2 )
HSP 1 Score: 734.2 bits (1894), Expect = 1.6e-211
Identity = 447/1140 (39.21%), Postives = 657/1140 (57.63%), Query Frame = 0
Query: 4 TIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAAGHV 63
T++I + + A+E+ FLE H G+ TV A+ I RD FA VQF++ L
Sbjct: 11 TVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTT-LEVKSR 70
Query: 64 VSQAAAEERLWYGSSYLKAREA-DRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWKRE 123
+++ +L + + L+ EA D R +R++++ L +G S EK
Sbjct: 71 AQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEK-------R 130
Query: 124 NCSVEFGSGVR----------KLFVYVNLE-YKMEVWFESILRVQLRCPPDGASKF--LL 183
C++E GVR + +V+ + + YK+EV FE I+ C AS+ L
Sbjct: 131 FCALEKWDGVRCWILTEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFL 190
Query: 184 IQLQRAPRIFKKSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCL 243
++L+ P++FK+ + ++ S RF + WIR DF+ S IG S CL
Sbjct: 191 LKLKYGPKVFKRVTVHIATKFKSDRYRFC---KEDFDFMWIRTTDFSGSKSIGTSTCFCL 250
Query: 244 ELSPGDQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPP-QGFDFSFKILFKI 303
E+ G + F L Y E G +F+S + +VP++ G +F ++ILF++
Sbjct: 251 EVHNGSTMLDIFSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQL 310
Query: 304 NALVQHGYLP-GPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLS 363
NALV + D E +++ + L+KL Y+P ++K Q S
Sbjct: 311 NALVHAQKISLFAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQS 370
Query: 364 YYNSKQLPWKPDIS----LDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNF 423
K++ P + + ++ R +TPSK+Y GPE +N VV+ +++ + +F
Sbjct: 371 VV--KKMKHSPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDF 430
Query: 424 IRVSFVDEELDKIRSIDLSPRTST---ANGERTRVYDRILSTLRNGIVIGDKKFEFLAFS 483
+RV+FV+E+ K+ + LS + RT +Y+R+LS L GI +G K+FEFLAFS
Sbjct: 431 MRVTFVEEDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFS 490
Query: 484 ASQLRENSVWMFASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHE 543
ASQLR NSVWMFAS E + A DIREWMG FR+IR+++K AAR+GQ F +SR+TL V +
Sbjct: 491 ASQLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQD 550
Query: 544 IEVITDVEVETNNVTYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAI 603
+E I D+EV T+ YCFSDGIGKIS A++VA++CGL +H PSAFQIRY GYKGV+A+
Sbjct: 551 VEQIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQKCGL-SHVPSAFQIRYGGYKGVIAV 610
Query: 604 DPTSEKKLSLRKSMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQK 663
D +S +KLSLR SMLK+ S + L+V W++ +PCFLNR++I LLSTLGI+D +FE Q
Sbjct: 611 DRSSFRKLSLRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQA 670
Query: 664 EAIYQLNCILKDPSRALEVLELMSPGEMTSVLKEMLLY-YKPNKEPFLNMMLRTFRADKL 723
+ L +L+D AL VL+ +S ++L +MLL Y P+ EP+L+MMLR +L
Sbjct: 671 VHLSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQL 730
Query: 724 LDLRTKSRIFVPKGRTMMGCLDETGTLKYGQVFVQCSVP------------GRSSESNFI 783
+L+++ RI VPKGR ++GC+DE G L+YGQV+V+ ++ + E +
Sbjct: 731 SELKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSV 790
Query: 784 PEGKVVVAKNPCLHPGDMRVLNAVDVKALHH--MVDCVVFPQKGQRPHPNECSGSDLDGD 843
GKVVV KNPCLHPGD+RVL+A+ +DC++FPQKG+RPHPNECSG DLDGD
Sbjct: 791 VIGKVVVTKNPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGD 850
Query: 844 LYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTV 903
+FV WD ++ PM Y + +DHDVT+EE+ ++ +YM++D LG I+ AH V
Sbjct: 851 QFFVSWDEKIIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLV 910
Query: 904 FADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESK 963
ADR+P KA S KC++LA L S AVDF KTG PA +P L+ EFPDF+E+ ++P Y S+
Sbjct: 911 HADRDPEKARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISE 970
Query: 964 GVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLG 1023
V GKL+R+VK S E + + A YD +E GFE ++ A + Y KL
Sbjct: 971 SVFGKLYRAVKS-SLAQRKPEAESEDTVA--YDVTLEEAGFESFIETAKAHRDMYGEKLT 1030
Query: 1024 NLMDYYGIRTEPELVSGNILRVGKSFDKRTD------MEQISHAMRSLRKEARAWFNEKG 1083
+LM YYG E E+++G IL+ + + R + ++I+ +++ L KEA WF +
Sbjct: 1031 SLMIYYGAANEEEILTG-ILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWFEKS- 1090
Query: 1084 SKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGMERDHFLSFPWCVSDKLIQIK 1100
C+ DE ++ ASAWY+VTY+ N E+ FLSFPW V D L+ IK
Sbjct: 1091 ----CE-----DEQQKKKLASAWYYVTYNP------NHRDEKLTFLSFPWIVGDVLLDIK 1114
BLAST of MC04g1001 vs. TAIR 10
Match:
AT3G49500.1 (RNA-dependent RNA polymerase 6 )
HSP 1 Score: 565.1 bits (1455), Expect = 1.3e-160
Identity = 383/1068 (35.86%), Postives = 566/1068 (53.00%), Query Frame = 0
Query: 98 RMENLTLHLGSQISKEKLSVIWKRENC-----------------SVEFGSGVRKLFVYVN 157
++ +TL +G+ +S++ V W+ E S F + +N
Sbjct: 131 KLAGITLEIGTLVSRDDFFVSWRAEGVDFLVDPFDNTCKFCFRKSTAFSFKDAVMHAVIN 190
Query: 158 LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAPRIFKKSSSSSSSSSSSSSSRFYFK 217
+YK+E+ I V+ G L++QL +PR++ +++ +
Sbjct: 191 CDYKLELLVRDIQTVRQYKTLHGF--VLILQLASSPRVWYRTADDDIYDTVPG------- 250
Query: 218 ESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ------LPSFFKTLVGYEEIYGPF 277
+ WIR DFT IG+ + + +SP + L F V E + P
Sbjct: 251 DLLDDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERVRWPP 310
Query: 278 NLETGSSFSS-TSNLVPIVTPPQGFDFSFKILFKINALVQHGYLPGPLLDDEFFRLVDPS 337
+ F S+ + +G SF+I+F +N+++ G L + FF L+
Sbjct: 311 RIRNEPCFGEPVSDHFFCIHHKEG--ISFEIMFLVNSVLHRGVFNQFQLTERFFDLL--- 370
Query: 338 RFRPEYIERA-LEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPWKPDISLDDGLVYVHRV 397
R +P+ + A L+ L K ++ K LK + +L + S D + + R+
Sbjct: 371 RNQPKDVNIASLKHLCTYKRPVFDAYKRLKLVQEWIQKNPKLLGSHEQSED--ISEIRRL 430
Query: 398 QITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSIDLS--------PR 457
ITP++ Y PE LSNRV+R+Y + F+RV+F+DE + I S LS
Sbjct: 431 VITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAPIVKDL 490
Query: 458 TSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSAADIR 517
TS++ ++T V+ R+ S L +G + +K+ FLAFSA+QLR+ S W FA +DI+
Sbjct: 491 TSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTRVSDIK 550
Query: 518 EWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDV-EVETNNVTYCFSDGIG 577
WMG F+ +NVAK AAR+G F S+ T+ V HE++ T+V ++E N Y FSDGIG
Sbjct: 551 TWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVD--TEVPDIERNG--YVFSDGIG 610
Query: 578 KISETLAEKVAERCGL-INHTPSAFQIRYAGYKGVVAIDPTSEK--KLSLRKSMLKYKSL 637
I+ LA++V E+ L ++++P A+QIRYAG+KGVVA P+ +L+LR SM K+ S
Sbjct: 611 TITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSMKKFFSK 670
Query: 638 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVL 697
T L++ +W+++ P FLNRQ+I LLS LG+ D +F Q+ +Y+LN IL D A EVL
Sbjct: 671 HTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTDVAFEVL 730
Query: 698 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 757
+ + + +KP EP L ML + R +L LR KSRIFV GR +MGCL
Sbjct: 731 TASCAEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSGRWLMGCL 790
Query: 758 DETGTLKYGQVFVQCSVP----------GRSSESNF---IPEGKVVVAKNPCLHPGDMRV 817
DE G L++GQ F+Q S P R E+ + +G V +AKNPCLHPGD+R+
Sbjct: 791 DEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDLEVVKGYVAIAKNPCLHPGDVRI 850
Query: 818 LNAVDVKALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVK----P 877
L AVDV LHHM DC++FPQKG RPH NE SGSDLDGDLYFV WD +L I P +
Sbjct: 851 LEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKL--IPPNRKSYPA 910
Query: 878 MSYKPAPTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLA 937
M Y A L V +++ ++ A + N+ LG I NAH V ADR AM +C+ LA
Sbjct: 911 MHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDEECLLLA 970
Query: 938 KLFSIAVDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDV-SFNT 997
+L + AVDFPKTG ++P L+ +PDFM K D Y+S ++G+L+R VK+V +
Sbjct: 971 ELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKEVYDEDA 1030
Query: 998 NTIETYTREVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSG 1057
+ + +A YD +EI GFE + EA+ K YD +L L+ Y ++ E E+V+G
Sbjct: 1031 EASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKEEEIVTG 1090
Query: 1058 NILRVGKSFDKRTD--MEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEE----F 1100
+I + K K+ E++ H+ SL+KE R F E D +N E+E+
Sbjct: 1091 HIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEE-----TIPDHENLSEEEKNILYE 1150
BLAST of MC04g1001 vs. TAIR 10
Match:
AT2G19930.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 129.4 bits (324), Expect = 1.8e-29
Identity = 179/698 (25.64%), Postives = 279/698 (39.97%), Query Frame = 0
Query: 335 YEPQKW--LKQQYLSYYNSKQLPWKPDISLDDGLVYVHRVQITPSKVY-------FRGPE 394
YE W L + Y + + L W D G + ++ + P+ Y +GP
Sbjct: 174 YEAAVWDRLGRHYCPQKDRRMLQW------DSGKTHYYQCNVAPNGSYTFKVLSALQGPL 233
Query: 395 ANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSIDLSPRTSTANGERTRVYDRILSTLRN 454
+ + + D DN + V F D+ +ST + + Y I +N
Sbjct: 234 LEHTGTHLHKVLGD-DNVLTVKFA----------DVQKSSSTYSIDHYFTYKGI---AKN 293
Query: 455 GIVIGDKKFEFLAFSASQLRENSVWMFASR----------------------EGLSAADI 514
GI+IG ++++F F E + + G S +
Sbjct: 294 GIMIGLRRYQFFVFKDGGKEEKKKDLSTKKVKCYFIRTDSTAFYDMQNPYILTGKSIYEA 353
Query: 515 REWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEI--EVITDVE---------VETN 574
R + +A Y AR S KTL V+ I + I D+ ++ N
Sbjct: 354 RMHFMHVHRAPTLANYMARFSLIL-SKTKTLEVDMTGITFDQIDDIHCHDQDGKDVLDKN 413
Query: 575 NVTYCFSDGIGKISETLA---------------EKVAERCGLINHTPSAFQIRYAGY--K 634
SDG G ISE LA E + E C F++ Y GY K
Sbjct: 414 KKPCIHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEACYQDPPLLIQFRMFYDGYAVK 473
Query: 635 GVVAID-PTSEKKLSLRKSMLKYKSLDTQLDVLAWSKYLPCF----------LNRQVINL 694
G ++ + + +R SM+K S D L + L L++ ++ L
Sbjct: 474 GTFLLNKKLCPRTVQVRPSMIKV-SKDPSLSNFSTFNALEVVTTSNPPKRTKLSKNLVAL 533
Query: 695 LSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVLELMSPGEMTSV-LKEMLLYYKPNK 754
LS GI + F + + I + AL ++ GEM +M+L P
Sbjct: 534 LSYGGIPNEFFLDILLNTLEESKSIFYNKRAALNA--ALNYGEMDDQNAAQMILVGIPLD 593
Query: 755 EPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETGTLKYGQVFVQCSVPGRSSE 814
EP L L + DL+ ++ V + +MG +D TG LK +V V E
Sbjct: 594 EPHLKNYLSILLKTEKNDLKA-GKLPVTESYYLMGTVDPTGALKEDEVCV-------ILE 653
Query: 815 SNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMVD----CVVFPQKGQRPHPNECSG 874
S I G+V+V +NP LH GD+ +L A VKAL V V FPQKG R +E +G
Sbjct: 654 SGQI-SGEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQKGPRSLGDEIAG 713
Query: 875 SDLDGDLYFVCWDPE-LNCIRPVKP-MSYKP--------APTIQLDHDVTIEEVQEYM-- 934
D DGD+YF+ +PE L +P +P +S P AP+ QL + EE+ E
Sbjct: 714 GDFDGDMYFISRNPELLENFKPSEPWVSLTPPSKSNSGRAPS-QLSPEELEEELFEMFLT 773
Query: 935 ANYMVNDGLGAIANAHTVFADR---------EPRKAMSAKCIKLAKLFSIAVDFPKTGVP 937
A + ++ +G A++ DR E + M K ++L ++ A+D PK G
Sbjct: 774 AGFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYDALDAPKKGDK 833
BLAST of MC04g1001 vs. TAIR 10
Match:
AT2G19920.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 119.4 bits (298), Expect = 1.8e-26
Identity = 150/593 (25.30%), Postives = 249/593 (41.99%), Query Frame = 0
Query: 436 YDRILSTLRNGIVIGDKKFEFLAF----SASQLRENS---VWMFASREG----------- 495
Y + +NGI++G ++++F F A + + NS V + R G
Sbjct: 268 YSKYKEIAKNGIMVGLRRYQFFVFKDGGKAEKKKRNSTKQVKCYFIRTGSTASSDMENPY 327
Query: 496 -LSAADIREWMGDFRQIRNV---AKYAARLGQSFGSSRKTLCVEEHEIEV--ITDVE--- 555
LS I E F + + A Y AR ++K L V+ EI V I D+
Sbjct: 328 ILSGMSIHEARMHFMHVHTLPSPANYMARFSLILSKTKK-LEVDMTEITVMQIDDIHCHD 387
Query: 556 ------VETNNVTYCFSDGIGKISETLA---------------EKVAERCGLINHTPSAF 615
++ N SDG G ISE LA + C + P
Sbjct: 388 QSNNDVLDKNGKPRIHSDGTGYISEDLARMCPLNIFKGKSMRSNNIQGTC--VQEPPLLI 447
Query: 616 QIRY----AGYKGVVAIDPT-SEKKLSLRKSMLK-YK-------SLDTQLDVLAWSK-YL 675
QIR + KG+ ++ + + +R SM+K YK S L+V+ S
Sbjct: 448 QIRMFNDGSAVKGIFLLNKNLPPQTVQVRPSMIKVYKDKNLSNFSTFNSLEVVTTSNPPK 507
Query: 676 PCFLNRQVINLLSTLGIKDRVF-------EKKQKEAIYQLNCILKDPSRALEVLELMSPG 735
L++ ++ LLS G+ + F +K+K +++ K AL +
Sbjct: 508 RAKLSKNLVALLSYGGVPNDFFLDILLNTLEKKKTIFFKVRAAGK---AALHYGNMDDKN 567
Query: 736 EMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETGTL 795
+ +M++ P EP+L L + DL+ ++ + + +MG +D TG L
Sbjct: 568 AL-----QMIMAGIPLDEPYLKHYLSKLLKLEKDDLKA-GKLPIDESYYLMGTVDPTGEL 627
Query: 796 KYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMVD----C 855
K +V S V+V +NP LH GD+ +L A VK+L V
Sbjct: 628 KEDEV------------SGLAKSQDVLVYRNPGLHFGDIHILKATYVKSLEQYVGNSKYG 687
Query: 856 VVFPQKGQRPHPNECSGSDLDGDLYFVCWDPE-LNCIRPVKP-MSYKPAPTI---QLDHD 915
V FPQKG R +E +G D DGD+YF+ +P+ L +P +P +S P I + +
Sbjct: 688 VFFPQKGPRSLGDEIAGGDFDGDMYFISRNPKLLEHYKPSEPWVSSSPRSKIYTGRQPSE 747
Query: 916 VTIEEVQEYMANYMVNDG------LGAIANAHTVFADR---------EPRKAMSAKCIKL 936
++ E+++E + + G +G A++ DR + + M K +KL
Sbjct: 748 LSPEQLEEELFKIFLKTGFSPSSVIGQAADSWLAIMDRFLTLGDENVKEKAEMKKKMLKL 807
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9LQV2 | 0.0e+00 | 57.93 | RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1 | [more] |
Q0DXS3 | 1.5e-267 | 62.64 | Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
O82504 | 2.2e-210 | 39.21 | RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1 | [more] |
Q7XM31 | 8.7e-199 | 38.45 | Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
Q9SG02 | 1.8e-159 | 35.86 | RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_022146462.1 | 0.0 | 99.91 | probable RNA-dependent RNA polymerase 1 [Momordica charantia] | [more] |
XP_038875555.1 | 0.0 | 79.30 | probable RNA-dependent RNA polymerase 1 [Benincasa hispida] | [more] |
XP_022994004.1 | 0.0 | 78.61 | probable RNA-dependent RNA polymerase 1 [Cucurbita maxima] | [more] |
XP_022938807.1 | 0.0 | 78.78 | probable RNA-dependent RNA polymerase 1 [Cucurbita moschata] | [more] |
KAG6579193.1 | 0.0 | 78.87 | RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1CZM7 | 0.0 | 99.91 | RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015673 PE=3... | [more] |
A0A6J1K1Q3 | 0.0 | 78.61 | RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111489826 PE=3 SV... | [more] |
A0A6J1FF44 | 0.0 | 78.78 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111444909 PE=3 ... | [more] |
A0A6J1JTB1 | 0.0 | 78.39 | RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111487656 PE=3 SV... | [more] |
A0A6J1EB47 | 0.0 | 78.07 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111430971 PE=3 ... | [more] |