MC04g0896 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0896
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein NRT1/ PTR FAMILY 3.1
LocationMC04: 16230824 .. 16235850 (+)
RNA-Seq ExpressionMC04g0896
SyntenyMC04g0896
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AACGGAAATTAATATTTTGACCATTACCATTTTTTGAGTTCAATAACTACGAGTGAGATCAAATATTTGAAAGGGTAATATGTATCTTTATCGCCTCGTTATCAAAAATAATTTGACATTACCTAAATTAACCTTTCAATCTTTTAAATAAAGGCAACAGTGAAATTTTTTTTTTTTAATTAGAACCAAAGAATTATAAGGAAAATAAAGATGGAATTTGTTTTCCCCTTTTAATTTTGCTTAAGGATAATATTTTCCCAACGTTGAACGACCCACATTTTTCCTCGAGGAATCTTTTTGTCTCCGCCGCAAGTTCCCTGTCTCCGGCAGCCCCATTCTCCGGCCACCGCCGCTTCTTTCCCTTTATTTAACTATCCCACACCTTTTTTCTCTCTCTCTCCAATAATTCTGAAACATAAGTAGGCAGCTTAACTTTTACTGGTTTGATTTCCATTTTTTCTTAAGGTTATAATCCTCTCATAACAATGTTTAATTTCAATCAATCATAATAAGCCATTTAACGATCATCATCTACCATTTATTAAACGAATCCAATCCCTACAAAATACTAAAGAAAAATTCCCATATTATATGTGGCCATAAATTTCACACAATACTTCCAATTTTGTCTTATCTCTTGGTGAAACTTATAAACAGTGTTAATCTTGTGATGTTTATCAGCTGCCTATAAACAATCCAAAAAAACAAAAAATCAGCTGCCTATAAACACCGAGAGCTTGCACAAGCCATTCAGCAGCAGCAAGAACCTTGAGGAACCCAACAATGGAGGAGGAGCAAACTAAAAATAAGCATGCCGGAAGAAAGAAGGGTGGCCTTATCACTATGCCCTTCATCTTCGGTACTATTCTTCTAATTTTTCTAGATCTGTTTTCTTTTTCTTTTTTTTTGGTGGGGTTTTGATTTTGCTTTTGGTTCATGGTTGAAAATTAAAGCGAATGAGATCTGCGAGAAGATTGCTGTGGTTGGGTTTAATACCAATATGATTAGCTACTTGACGAAGCAGCTCCATATGCCCTTAACCCAAGCAGCCAACACCATCACCAACTTCAGCGGAACCGCCAGCTTGACGCCGCTCATCGGAGCCTTCATCGCCGACTCCTATGCCGGCCGCTTTTGGACCATCACTGTCGCCTCCATTCTTTACCAGATAGTAAGTAATCGGTTTAGTAACATTTTTGTTTCTTATTTCCAGTTCAATCTTGATTGTGTTTCTTCGTTTCAAATTCATTAAATCCTAAACTTATTTTAGGTTGAGTTGACATAAAAATGAATAAACTATCACTCAAATAAAAAATTTAACATAATGCATATATAGATGTCTCAACATTATCTTCACTTTGACAGAAGATGAATAACTATCCTCGACCCAAAAACTTAAGAGGGTAGATTGAGTAAATTTAGTTTATTTATATGATTCTCAACCGTGTTAACGGAAACTTTTGAGTTGCATGATAGTTTATTTATCAAAAACTTCTTAATAAAGTGTGGTTATGTACGGTAGGGTATGACAATCCTGACGACATCGGCAGTGGTGCCGACCCTGAGGCCGCCACCGTGCAACGGCGGAGAAGTGTGCTCGCAGGCGGACGGCGGGCAGCTGGGAATCCTATACATATCGCTTCTGTTGACGGCGCTCGGGTCGGGCGGGATCCGGCCGTGCGTGGTTTCGTTCGGGGCGGACCAGTTCGAGGAGTCGGATCCGAAGCGGGGGATGAAGACGTACAAGTATTTCAATTGGTACTATTTTGCGATGGGGGCGTCGATGCTGGTGGCGGTGACGGTGCTGGTGTACGTGCAGGATAACATCGGCTGGGGCTGGGGCTTCGGGATTCCCACCATCGCCATGTTCCTCTCCATCGTCACCTTCATTGTCGGGTACCCGATTTATAGGCACTTGGATCCGTCGGGTAGCCCGTTTACCCGTTTGTTGCAGGTCGGGGTTGCCGCCTACCGGAAGAGGAAACTCGACCCGGTTTCTGATTACAAGATGCTGTATCAGAATCATGAGATTGATGACCCAATTTCAAGAGATGGGAAGCTCCTTCATACCAAACATATGAAGTAATTCTCTCTCCTCTTTCTCTCTCTAACTCTTTTTTTTTTTGCTCTAATTAATAATTGTCTTTAGAACTCAGATAGATATTTTTTTTGAATTCGCTAAAAGTTTTGATAGGTAGGAGAGAAAAAAATAGAAAAATAAATATTTTTCTTGCATTTTAAGAATGAAAAATTGTTATTTTGTGGTGGCCAACCAGAAAGTACAAATAAATCATTCTAACATTCAAGTTACATAATTTGTCCTTTCAAACGTAACTTAATAGATGAAGTATTGACTATCAAGTTCGATAGTTGGATTATTGAACCAAAAAGTAATTACTATCTAAAAAGAGAGAAAATAAATAGAATATTCTAGCTATAAAATATACCAAACTTTCAACCAAACACTTGGAACATAAGTATATTAGTCAGTTAAAACATTTTAATCTCCATCTTCATTGAGCTTAAAAATAATTAGAATCCTAATCACCTCTATGAATTAATTTCCTATTTGTTTTCTTAACAATCAAATGCAGTAGGGTGCCAATATGACCTTAGCTCAACTGTAATTGACATATATCTCCAATCAATATATTGTTAGTTCAATCTCCTACCCCTACATGTTGTATTTAAAAAAAATAAAAAATAAAAAAAGTGGTAGGATGCCAACTAGAACATAGATCAACTGTTTAAGGTACCTACGTCTAAACTAGAATATCATGAGTTCGAATTCCTACTCACGCATATTGTACTAAAAAAAGTGATATGGTCGGTTCATGAGATCAAAGTGTGTACAAATTGGTTTGAGCGTTCGCGTTAATATCAAAAAAAAAATTAAAAATACACCATCATTGTTGTGTTTATATTTAATTTGAGATCCCAATCTAGTAGATGGAATAAAAAGGAAATTATTTAGAATGTGGACACAAGTTTGAAAGAAAACTAGATGGCATTTGTTTGTCATTGAGCAGCGAGGGGTGTGGAGATGAGTGAGTCTGATCAACTTTGCCCTCTTTTTGACCAACAACACATAAGACTTTTTGGATTCTCTTTTTTGTGTTCTTGCTAAACAACAAAACCACCTCTGTTTCACATACAATATTTATCCCAACTTCCCCCCCTTTTTAATTCTTAAATTATATCCTCCATATTTCCTTTTTTTTCTTTTTTACTTATCTAATCTTTTTCTCTTGCTTTTTACTGTTCACAAACTTAATTTTCTGTTTTCTTTTTATATTCGACAGGAAAAATTACTTTTATTGCTGAGTCGTCTCTATCATTGCCTTTTTTTCTTCTTTTCTACTACTGGATTTTTTCTTTTTTCTTTTCCAATATATATTTCATCATAGTTGTTTTATTAGGATCAAACTGCACAAATCACTCCAAAAGTGGGGTGGTTGTTCCACCCTAAAAATTAAACTCTCACAGATTCATAAACAAAAAATTAGACTCCGAAACTGTCAATAATAAAAATTAAACCTTAAACTTTTGTAAGGGAGAAAATTGCACAATTAGTAAAAATTAAATCCTCACTTTAAAACAAAAATGGAGTAAATAAACTCAACTTTCAACTTTCTTTTAAGTCTGTTTACCTTTTACTTTTGAAAGTTTAGGGTTCGAATTTATTTATTTCATTTATGATAGTTGTTACAATTACACCCTTAAACTTTCAATAATTAAAATTAAACCCTAAAACTTTCTTGCACCCGTAAACTTTCAATTCGTAAAAATTAAACCCTCGCTGTGAAAGATAAATAGAGTAAATAGATCCAACCTTTCTCTTTTAAGTTTGAGGGTGTAATTTTTACTTTTGAAAGTTTAGATATCTATTTTACTAGTGATTATGTGATATTCTACCCTCATCGGGTCCTTTTTAGCTAGGCTTGAGAATCCATAGCTTGATTATAAGTTCTATTTAATCACGCAGATTTCTAGACAAGGCGGCAATAGTAACAGAAGAAGACGAGCTGAAAACGGGCCGAAAGCCCAATCTATGGAAGCTGAACACAGTCCACCGAGTGGAGGAGCTGAAGTCCGTAATCCGAATGGGCCCAATATGGGCCTCAGGAATAATCCTAATCACGGCCTATTCCCAACAGAACACATTCTCCCTCCAACAAGCCTCAACAATGAACCGCCACGTCACCGAATCCTTCCAAATCCCGGCCGGCTCAATGACCGTTTTCACCCTCCTCACAATGCTCGTCACCGTCGCCCTATACGACCGCGTTTTTGTCCCCATCGCCCGCCGCTTCACCGGCCTCGACCGCGGCATCACCTTCCTCCGCCGCATGGGCATCGGCCTCGTCATCTCCGTATTCGCCACCCTCATCGCCGGCTTCGTCGAGAAGAAGCGGAAACACGCCGCCGCCGGTGACCCCATTTCAGTCTTCTGGCTGGTGCCGCAGTACAGCCTCCACGGCATGGCGGAGGCGTTCATGTCCATCGGCCACCTCGAGTTCTTCTACGACCAGTCGCCGGAGAGCATGAGGAGCACCGCCATGGCGCTCTTCTGGACCTCCATCTCCGCCGGCAACTACTTGAGCACGCTCCTCGTTACGCTCGTGCATAAGTTCACGGCCGGCCCTGGGGGATCCAACTGGCTAAGGAATGATAATCTGAATTTGGGGAAGCTCGAGAATTTCTACTGGTTGATTACTCTGCTGCAGGTTTTTAATCTTGTTTATTATGTGTTTTGTGCCAAAGTTTACACTTTTAAGCCGTTGGAGCTTCAGGGGAAGGTTGTAGATAGCTCTAAGGTGGATGAAGTTCAACTTGTCAACCATGTTTAGATCACCTGAAAAATGTTAGGAGGATCGAATTCACCGCATCTTCAATGAAGATATATATCAATTAACTTGTGTCTTATCGAATAGTTTAAATAAATCAATGAAAAAAATCAATCACATTACATGAAAATTTATACTGATATGTGTAGGCAAGTACTATTAATGCATGAAAATGGTTTC

mRNA sequence

AACGGAAATTAATATTTTGACCATTACCATTTTTTGAGTTCAATAACTACGAGTGAGATCAAATATTTGAAAGGGTAATATGTATCTTTATCGCCTCGTTATCAAAAATAATTTGACATTACCTAAATTAACCTTTCAATCTTTTAAATAAAGGCAACAGTGAAATTTTTTTTTTTTAATTAGAACCAAAGAATTATAAGGAAAATAAAGATGGAATTTGTTTTCCCCTTTTAATTTTGCTTAAGGATAATATTTTCCCAACGTTGAACGACCCACATTTTTCCTCGAGGAATCTTTTTGTCTCCGCCGCAAGTTCCCTGTCTCCGGCAGCCCCATTCTCCGGCCACCGCCGCTTCTTTCCCTTTATTTAACTATCCCACACCTTTTTTCTCTCTCTCTCCAATAATTCTGAAACATAAGTAGGCAGCTTAACTTTTACTGGTTTGATTTCCATTTTTTCTTAAGGTTATAATCCTCTCATAACAATGTTTAATTTCAATCAATCATAATAAGCCATTTAACGATCATCATCTACCATTTATTAAACGAATCCAATCCCTACAAAATACTAAAGAAAAATTCCCATATTATATGTGGCCATAAATTTCACACAATACTTCCAATTTTGTCTTATCTCTTGGTGAAACTTATAAACAGTGTTAATCTTGTGATGTTTATCAGCTGCCTATAAACAATCCAAAAAAACAAAAAATCAGCTGCCTATAAACACCGAGAGCTTGCACAAGCCATTCAGCAGCAGCAAGAACCTTGAGGAACCCAACAATGGAGGAGGAGCAAACTAAAAATAAGCATGCCGGAAGAAAGAAGGGTGGCCTTATCACTATGCCCTTCATCTTCGCGAATGAGATCTGCGAGAAGATTGCTGTGGTTGGGTTTAATACCAATATGATTAGCTACTTGACGAAGCAGCTCCATATGCCCTTAACCCAAGCAGCCAACACCATCACCAACTTCAGCGGAACCGCCAGCTTGACGCCGCTCATCGGAGCCTTCATCGCCGACTCCTATGCCGGCCGCTTTTGGACCATCACTGTCGCCTCCATTCTTTACCAGATAGGTATGACAATCCTGACGACATCGGCAGTGGTGCCGACCCTGAGGCCGCCACCGTGCAACGGCGGAGAAGTGTGCTCGCAGGCGGACGGCGGGCAGCTGGGAATCCTATACATATCGCTTCTGTTGACGGCGCTCGGGTCGGGCGGGATCCGGCCGTGCGTGGTTTCGTTCGGGGCGGACCAGTTCGAGGAGTCGGATCCGAAGCGGGGGATGAAGACGTACAAGTATTTCAATTGGTACTATTTTGCGATGGGGGCGTCGATGCTGGTGGCGGTGACGGTGCTGGTGTACGTGCAGGATAACATCGGCTGGGGCTGGGGCTTCGGGATTCCCACCATCGCCATGTTCCTCTCCATCGTCACCTTCATTGTCGGGTACCCGATTTATAGGCACTTGGATCCGTCGGGTAGCCCGTTTACCCGTTTGTTGCAGGTCGGGGTTGCCGCCTACCGGAAGAGGAAACTCGACCCGGTTTCTGATTACAAGATGCTGTATCAGAATCATGAGATTGATGACCCAATTTCAAGAGATGGGAAGCTCCTTCATACCAAACATATGAAATTTCTAGACAAGGCGGCAATAGTAACAGAAGAAGACGAGCTGAAAACGGGCCGAAAGCCCAATCTATGGAAGCTGAACACAGTCCACCGAGTGGAGGAGCTGAAGTCCGTAATCCGAATGGGCCCAATATGGGCCTCAGGAATAATCCTAATCACGGCCTATTCCCAACAGAACACATTCTCCCTCCAACAAGCCTCAACAATGAACCGCCACGTCACCGAATCCTTCCAAATCCCGGCCGGCTCAATGACCGTTTTCACCCTCCTCACAATGCTCGTCACCGTCGCCCTATACGACCGCGTTTTTGTCCCCATCGCCCGCCGCTTCACCGGCCTCGACCGCGGCATCACCTTCCTCCGCCGCATGGGCATCGGCCTCGTCATCTCCGTATTCGCCACCCTCATCGCCGGCTTCGTCGAGAAGAAGCGGAAACACGCCGCCGCCGGTGACCCCATTTCAGTCTTCTGGCTGGTGCCGCAGTACAGCCTCCACGGCATGGCGGAGGCGTTCATGTCCATCGGCCACCTCGAGTTCTTCTACGACCAGTCGCCGGAGAGCATGAGGAGCACCGCCATGGCGCTCTTCTGGACCTCCATCTCCGCCGGCAACTACTTGAGCACGCTCCTCGTTACGCTCGTGCATAAGTTCACGGCCGGCCCTGGGGGATCCAACTGGCTAAGGAATGATAATCTGAATTTGGGGAAGCTCGAGAATTTCTACTGGTTGATTACTCTGCTGCAGGTTTTTAATCTTGTTTATTATGTGTTTTGTGCCAAAGTTTACACTTTTAAGCCGTTGGAGCTTCAGGGGAAGGTTGTAGATAGCTCTAAGGTGGATGAAGTTCAACTTGTCAACCATGTTTAGATCACCTGAAAAATGTTAGGAGGATCGAATTCACCGCATCTTCAATGAAGATATATATCAATTAACTTGTGTCTTATCGAATAGTTTAAATAAATCAATGAAAAAAATCAATCACATTACATGAAAATTTATACTGATATGTGTAGGCAAGTACTATTAATGCATGAAAATGGTTTC

Coding sequence (CDS)

ATGGAGGAGGAGCAAACTAAAAATAAGCATGCCGGAAGAAAGAAGGGTGGCCTTATCACTATGCCCTTCATCTTCGCGAATGAGATCTGCGAGAAGATTGCTGTGGTTGGGTTTAATACCAATATGATTAGCTACTTGACGAAGCAGCTCCATATGCCCTTAACCCAAGCAGCCAACACCATCACCAACTTCAGCGGAACCGCCAGCTTGACGCCGCTCATCGGAGCCTTCATCGCCGACTCCTATGCCGGCCGCTTTTGGACCATCACTGTCGCCTCCATTCTTTACCAGATAGGTATGACAATCCTGACGACATCGGCAGTGGTGCCGACCCTGAGGCCGCCACCGTGCAACGGCGGAGAAGTGTGCTCGCAGGCGGACGGCGGGCAGCTGGGAATCCTATACATATCGCTTCTGTTGACGGCGCTCGGGTCGGGCGGGATCCGGCCGTGCGTGGTTTCGTTCGGGGCGGACCAGTTCGAGGAGTCGGATCCGAAGCGGGGGATGAAGACGTACAAGTATTTCAATTGGTACTATTTTGCGATGGGGGCGTCGATGCTGGTGGCGGTGACGGTGCTGGTGTACGTGCAGGATAACATCGGCTGGGGCTGGGGCTTCGGGATTCCCACCATCGCCATGTTCCTCTCCATCGTCACCTTCATTGTCGGGTACCCGATTTATAGGCACTTGGATCCGTCGGGTAGCCCGTTTACCCGTTTGTTGCAGGTCGGGGTTGCCGCCTACCGGAAGAGGAAACTCGACCCGGTTTCTGATTACAAGATGCTGTATCAGAATCATGAGATTGATGACCCAATTTCAAGAGATGGGAAGCTCCTTCATACCAAACATATGAAATTTCTAGACAAGGCGGCAATAGTAACAGAAGAAGACGAGCTGAAAACGGGCCGAAAGCCCAATCTATGGAAGCTGAACACAGTCCACCGAGTGGAGGAGCTGAAGTCCGTAATCCGAATGGGCCCAATATGGGCCTCAGGAATAATCCTAATCACGGCCTATTCCCAACAGAACACATTCTCCCTCCAACAAGCCTCAACAATGAACCGCCACGTCACCGAATCCTTCCAAATCCCGGCCGGCTCAATGACCGTTTTCACCCTCCTCACAATGCTCGTCACCGTCGCCCTATACGACCGCGTTTTTGTCCCCATCGCCCGCCGCTTCACCGGCCTCGACCGCGGCATCACCTTCCTCCGCCGCATGGGCATCGGCCTCGTCATCTCCGTATTCGCCACCCTCATCGCCGGCTTCGTCGAGAAGAAGCGGAAACACGCCGCCGCCGGTGACCCCATTTCAGTCTTCTGGCTGGTGCCGCAGTACAGCCTCCACGGCATGGCGGAGGCGTTCATGTCCATCGGCCACCTCGAGTTCTTCTACGACCAGTCGCCGGAGAGCATGAGGAGCACCGCCATGGCGCTCTTCTGGACCTCCATCTCCGCCGGCAACTACTTGAGCACGCTCCTCGTTACGCTCGTGCATAAGTTCACGGCCGGCCCTGGGGGATCCAACTGGCTAAGGAATGATAATCTGAATTTGGGGAAGCTCGAGAATTTCTACTGGTTGATTACTCTGCTGCAGGTTTTTAATCTTGTTTATTATGTGTTTTGTGCCAAAGTTTACACTTTTAAGCCGTTGGAGCTTCAGGGGAAGGTTGTAGATAGCTCTAAGGTGGATGAAGTTCAACTTGTCAACCATGTTTAG

Protein sequence

MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGEVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESFQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIAGFVEKKRKHAAAGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYVFCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV
Homology
BLAST of MC04g0896 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 3.3e-242
Identity = 415/580 (71.55%), Postives = 485/580 (83.62%), Query Frame = 0

Query: 2   EEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTI 61
           EEE+  +    R KGGLITMPFIFANEICEK+AVVGF+ NMISYLT QLH+PLT+AANT+
Sbjct: 11  EEEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTL 70

Query: 62  TNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGE 121
           TNF+GT+SLTPL+GAFIADS+AGRFWTIT ASI+YQIGMT+LT SA++PTLRPPPC G E
Sbjct: 71  TNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEE 130

Query: 122 VCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFA 181
           VC  AD  QL ILY++LLL ALGSGGIRPCVV+FGADQF+ESDP +  KT+ YFNWYYF 
Sbjct: 131 VCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFC 190

Query: 182 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLL 241
           MGA++L+AVTVLV++QDN+GWG G GIPT+AMFLS++ F+ G+ +YRHL P+GSPFTRL+
Sbjct: 191 MGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLI 250

Query: 242 QVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKT 301
           QVGVAA+RKRKL  VSD  +LY N EID PIS  GKL HTKHM FLDKAAIVTEED LK 
Sbjct: 251 QVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIVTEEDNLKP 310

Query: 302 GRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESF 361
           G+ PN W+L+TVHRVEELKSVIRMGPI ASGI+LITAY+QQ TFSLQQA TMNRH+T SF
Sbjct: 311 GQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSF 370

Query: 362 QIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIA 421
           QIPAGSM+VFT + ML T+  YDRVFV +AR+FTGL+RGITFL RMGIG VIS+ ATL+A
Sbjct: 371 QIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVA 430

Query: 422 GFVEKKRKHAAAGD----------PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPES 481
           GFVE KRK  A             PIS  WL+PQY LHG+AEAFMSIGHLEFFYDQ+PES
Sbjct: 431 GFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPES 490

Query: 482 MRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLL 541
           MRSTA ALFW +IS GNY+STLLVTLVHKF+A P GSNWL ++NLN G+LE FYWLIT+L
Sbjct: 491 MRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVL 550

Query: 542 QVFNLVYYVFCAKVYTFKPLELQGKVVDSSKV-DEVQLVN 571
           Q  NLVYY++CAK+YT+KP+++     DSS V +E+QL N
Sbjct: 551 QAVNLVYYLWCAKIYTYKPVQVHHSKEDSSPVKEELQLSN 590

BLAST of MC04g0896 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 5.4e-136
Identity = 247/551 (44.83%), Postives = 359/551 (65.15%), Query Frame = 0

Query: 7   KNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTITNFSG 66
           KN     K G      FI  NE CE++A  G  TN+++YL  +L+     AAN +TN+SG
Sbjct: 17  KNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSG 76

Query: 67  TASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGEVCSQA 126
           T  +TPLIGAFIAD+Y GR+WTI     +Y  GMT+LT SA VP L+P  CN  + C   
Sbjct: 77  TCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN-ADTC-HP 136

Query: 127 DGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAMGASM 186
           +  Q  + +++L + ALG+GGI+PCV SFGADQF+E+D    +K   +FNW+YF++    
Sbjct: 137 NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGA 196

Query: 187 LVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQVGVA 246
           L+A TVLV++Q N+GWGWGFG+PT+AM +++  F  G   YR   P GSP TR+ QV VA
Sbjct: 197 LIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVA 256

Query: 247 AYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTGRKPN 306
           A+RK  +    D  +L++  + +  I    KL+HT ++KF DKAA+ ++ D +K G + N
Sbjct: 257 AFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDG-EVN 316

Query: 307 LWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESFQIPAG 366
            W+L +V +VEELKS+I + P+WA+GI+  T YSQ +T  + Q +TM++H+ ++F+IP+ 
Sbjct: 317 PWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSA 376

Query: 367 SMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIAGFVEK 426
           S+++F  +++L    +YD+  +P+AR+FT  +RG T L+RMGIGLV+S+FA + AG +E 
Sbjct: 377 SLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEV 436

Query: 427 KR-----KHAAAGDP---ISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMA 486
            R      H A       +S+FW +PQY L G AE F  IG LEFFYDQ+P++MRS   A
Sbjct: 437 VRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSA 496

Query: 487 LFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVY 546
           L  T+++ GNYLST+LVT+V K T   G   W+  DNLN G L+ F++L+  L   N + 
Sbjct: 497 LSLTTVALGNYLSTVLVTVVMKITKKNGKPGWI-PDNLNRGHLDYFFYLLATLSFLNFLV 556

Query: 547 YVFCAKVYTFK 550
           Y++ +K Y +K
Sbjct: 557 YLWISKRYKYK 563

BLAST of MC04g0896 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 5.4e-136
Identity = 255/535 (47.66%), Postives = 354/535 (66.17%), Query Frame = 0

Query: 23  FIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTITNFSGTASLTPLIGAFIADSY 82
           FI   E CE++A  G +TN+I+YL KQ++M    A+ +++N+SGT   TPLIGAFIAD+Y
Sbjct: 34  FILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSNWSGTCYATPLIGAFIADAY 93

Query: 83  AGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGEVCSQADGGQLGILYISLLLTA 142
            GR+WTI    ++Y  GMT+LT SA VP L  P C+ GE C  A  GQ  I +I+L L A
Sbjct: 94  LGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTCS-GETC-HATAGQTAITFIALYLIA 153

Query: 143 LGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAMGASMLVAVTVLVYVQDNIGW 202
           LG+GGI+PCV SFGADQF+++D K       +FNW+YF +    ++A +VLV++Q N+GW
Sbjct: 154 LGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGW 213

Query: 203 GWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQVGVAAYRKRKLDPVSDYKML 262
           GWG G+PT+AM +++V F  G   YR   P GSP TR+LQV VA+ RK K+    D  +L
Sbjct: 214 GWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVASCRKSKVKIPEDESLL 273

Query: 263 YQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTGRKPNLWKLNTVHRVEELKSV 322
           Y+N + +  I    KL HTK + F DKAA+ TE D  K   K + WKL TV +VEELK++
Sbjct: 274 YENQDAESSIIGSRKLEHTKILTFFDKAAVETESDN-KGAAKSSSWKLCTVTQVEELKAL 333

Query: 323 IRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESFQIPAGSMTVFTLLTMLVTVAL 382
           IR+ PIWA+GI+  + YSQ  T  + Q +T+++H+  +F+IP+ S+++F  L++L    +
Sbjct: 334 IRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPV 393

Query: 383 YDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIAGFVEKKR-----KHAAAGD-- 442
           YD++ VP AR++TG +RG T L+R+GIGLVIS+F+ + AG +E  R      H    +  
Sbjct: 394 YDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEVARLNYVQTHNLYNEET 453

Query: 443 -PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSISAGNYLSTLL 502
            P+++FW VPQY L G AE F  IG LEFFYDQ+P++MRS   AL  T+I+ GNYLST L
Sbjct: 454 IPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFL 513

Query: 503 VTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYVFCAKVYTFK 550
           VTLV K T   G   W+   NLN G L+ F+WL+  L   N + Y++ AK YT+K
Sbjct: 514 VTLVTKVTRSGGRPGWIAK-NLNNGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYK 563

BLAST of MC04g0896 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 1.9e-133
Identity = 254/541 (46.95%), Postives = 349/541 (64.51%), Query Frame = 0

Query: 8   NKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTITNFSGT 67
           N  + +K G     PFI  NE CE++A  G   N+I+Y T +LH     AA+ +  + GT
Sbjct: 46  NPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGT 105

Query: 68  ASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNG--GEVCSQ 127
             +TPLIGA IADSY GR+WTI   S +Y IGM +LT SA +P L+P  C G    +CS 
Sbjct: 106 CYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCSP 165

Query: 128 ADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAMGAS 187
           A   Q  + +  L L ALG+GGI+PCV SFGADQF+++DP+  ++   +FNW+YF++   
Sbjct: 166 ATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIG 225

Query: 188 MLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQVGV 247
             ++ T+LV+VQ+N+GWG GF IPT+ M +SI +F +G P+YR   P GSP TR+ QV V
Sbjct: 226 SFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLV 285

Query: 248 AAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTGRKP 307
           AAYRK KL+   D   LY+  E +  I+   K+ HT   KFLDKAA+++E  E K+G   
Sbjct: 286 AAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEY-ESKSGAFS 345

Query: 308 NLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESFQIPA 367
           N WKL TV +VEE+K++IRM PIWASGI+    YSQ +T  +QQ  +MNR +  SF+IP 
Sbjct: 346 NPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNR-IIRSFEIPP 405

Query: 368 GSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIAGFVE 427
            S  VF  L +L+++ +YDR  VP  RRFTG+ +G+T L+RMGIGL +SV +   A  VE
Sbjct: 406 ASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIVE 465

Query: 428 KKRKHAAAG-DPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSI 487
             R   A     +S+FW +PQY L G+AE F  IG +EFFYD+SP++MRS   AL   + 
Sbjct: 466 TVRLQLAQDFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSVCSALALLNT 525

Query: 488 SAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNL-VYYVFCA 545
           + G+YLS+L++TLV  FTA  G   W+  D+LN G L+ F+WL+  L + N+ VY + C 
Sbjct: 526 AVGSYLSSLILTLVAYFTALGGKDGWV-PDDLNKGHLDYFFWLLVSLGLVNIPVYALICV 583

BLAST of MC04g0896 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 2.4e-131
Identity = 252/545 (46.24%), Postives = 343/545 (62.94%), Query Frame = 0

Query: 14  KKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTITNFSGTASLTPL 73
           K G     PFI  NE CE++A  G   N+I+YLT +LH     AA  +T + GT  LTPL
Sbjct: 41  KTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLTPL 100

Query: 74  IGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGEVCSQADGGQLGI 133
           IGA +AD+Y GR+WTI   S +Y IGM+ LT SA VP L+P  C  G+ C  A   Q  +
Sbjct: 101 IGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECI-GDFCPSATPAQYAM 160

Query: 134 LYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAMGASMLVAVTVL 193
            +  L L ALG+GGI+PCV SFGADQF+++D +  ++   +FNW+YF++    LV+ ++L
Sbjct: 161 FFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSSSLL 220

Query: 194 VYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQVGVAAYRKRKL 253
           V++Q+N GWG GFGIPT+ M L+I +F  G P+YR   P GSP TR+ QV VA++RK  +
Sbjct: 221 VWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRKSSV 280

Query: 254 DPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTGRKPNLWKLNTV 313
               D  +LY+  + +  I+   K+ HT   ++LDKAA+++EE E K+G   N W+L TV
Sbjct: 281 KVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEE-ESKSGDYSNSWRLCTV 340

Query: 314 HRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESFQIPAGSMTVFTL 373
            +VEELK +IRM PIWASGII    Y+Q +T  +QQ   MN  +  SFQ+P  ++  F  
Sbjct: 341 TQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKI-GSFQLPPAALGTFDT 400

Query: 374 LTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIAGFVEKKRKHAA- 433
            ++++ V LYDR  VP+AR+FTG+D+G T ++RMGIGL +SV     A  VE  R H A 
Sbjct: 401 ASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMAN 460

Query: 434 --------AGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSI 493
                   A  PISV W +PQY + G AE F  IG LEFFYDQSP++MRS   AL   + 
Sbjct: 461 DLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTN 520

Query: 494 SAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYVFCAK 550
           + GNYLS+L++TLV  FT   G   W+ +DNLN G L+ F+WL+  L + N+  Y F A 
Sbjct: 521 ALGNYLSSLILTLVTYFTTRNGQEGWI-SDNLNSGHLDYFFWLLAGLSLVNMAVYFFSAA 580

BLAST of MC04g0896 vs. NCBI nr
Match: XP_022135586.1 (protein NRT1/ PTR FAMILY 3.1 [Momordica charantia])

HSP 1 Score: 1140 bits (2950), Expect = 0.0
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0

Query: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60
           MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT
Sbjct: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60

Query: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120
           ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG
Sbjct: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120

Query: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180
           EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF
Sbjct: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180

Query: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240
           AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL
Sbjct: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240

Query: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300
           LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK
Sbjct: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300

Query: 301 TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES 360
           TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES
Sbjct: 301 TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES 360

Query: 361 FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI 420
           FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI
Sbjct: 361 FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI 420

Query: 421 AGFVEKKRKHAAAGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALF 480
           AGFVEKKRKHAAAGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALF
Sbjct: 421 AGFVEKKRKHAAAGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALF 480

Query: 481 WTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYV 540
           WTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYV
Sbjct: 481 WTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYV 540

Query: 541 FCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572
           FCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV
Sbjct: 541 FCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572

BLAST of MC04g0896 vs. NCBI nr
Match: XP_022994014.1 (protein NRT1/ PTR FAMILY 3.1 [Cucurbita maxima])

HSP 1 Score: 998 bits (2580), Expect = 0.0
Identity = 493/582 (84.71%), Postives = 534/582 (91.75%), Query Frame = 0

Query: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60
           MEE+Q+   H  RKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLT+ ANT
Sbjct: 1   MEEQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANT 60

Query: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120
           ITNF+GTASLTPLIGAFIADSY GRFWTITVASILYQIGM  LTTSAVVP LRPPPC GG
Sbjct: 61  ITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCKGG 120

Query: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180
           E+C +A G QLGILY SLLLTALGSGGIRPCVVSFGADQF+ESDPK+G KTYKYFNWYYF
Sbjct: 121 EICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYF 180

Query: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240
           AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TF++GY IYRHLDPSGSPFTRL
Sbjct: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRL 240

Query: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300
           LQV VAA+RKRKL+ V +  +LYQNH+IDDPISRDGKLLHT HM+FLDKAAIVTE+D++K
Sbjct: 241 LQVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVK 300

Query: 301 TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES 360
            G KPNLW LNTVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSLQQ +TM+R +T+S
Sbjct: 301 VGEKPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLTDS 360

Query: 361 FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI 420
           F+IPAGSM+VFTLLTML+T+ALYDR+F+PIARRFTGLDRGITFL+RMGIGLVIS+FATL+
Sbjct: 361 FKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFATLV 420

Query: 421 AGFVEKKRKHAA----------AGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480
           AGFVE+KRKH A          +  PIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE
Sbjct: 421 AGFVERKRKHVAFIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480

Query: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITL 540
           SMRSTAMALFWTSISAGNYLSTLLVTLVHK+TAGP G NWLRNDN+N GKLENFYWL+TL
Sbjct: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLVTL 540

Query: 541 LQVFNLVYYVFCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572
           LQVFNLVYYV CAK+YTFKPLE+Q KVVDSSKVDEVQLVN V
Sbjct: 541 LQVFNLVYYVVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 582

BLAST of MC04g0896 vs. NCBI nr
Match: XP_023550855.1 (protein NRT1/ PTR FAMILY 3.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 996 bits (2574), Expect = 0.0
Identity = 490/582 (84.19%), Postives = 534/582 (91.75%), Query Frame = 0

Query: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60
           MEE+Q+   H  RKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLT+ ANT
Sbjct: 1   MEEQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANT 60

Query: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120
           ITNF+GTASLTPLIGAFIADSY GRFWTITVASILYQIGM  LTTSAVVP L+PPPC GG
Sbjct: 61  ITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLKPPPCKGG 120

Query: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180
           E+C +A G QLGILY SLLLTALGSGGIRPCVVSFGADQF+ESDPK+G KTYKYFNWYYF
Sbjct: 121 EICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYF 180

Query: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240
           AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TF++GY IYRHLDPSGSPFTRL
Sbjct: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRL 240

Query: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300
           LQV VAA+RKRKL+ V +  +LYQNH+IDDPISRDGKLLHT HM+FLDKAA+VTE+D++K
Sbjct: 241 LQVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAVVTEQDKVK 300

Query: 301 TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES 360
            G KPNLW LNTVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSLQQ +TM+R +T+S
Sbjct: 301 LGEKPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLTDS 360

Query: 361 FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI 420
           F+IPAGSM+VFTLLTML+T+ALYDR+F+PIARRFTGLDRGITFL+RMGIGLVIS+FATL+
Sbjct: 361 FKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFATLV 420

Query: 421 AGFVEKKRKHAA----------AGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480
           AGFVE+KRKH A          +  PIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE
Sbjct: 421 AGFVERKRKHVALIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480

Query: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITL 540
           SMRSTAMALFWTSISAGNYLSTLLVTLVHK+TAGP G NWLRNDN+N GKLENFYWL+TL
Sbjct: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLVTL 540

Query: 541 LQVFNLVYYVFCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572
           LQVFNLVYY+ CAK+YTFKPLE+Q KVVDSSKVDEVQLVN V
Sbjct: 541 LQVFNLVYYIVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 582

BLAST of MC04g0896 vs. NCBI nr
Match: XP_022939103.1 (protein NRT1/ PTR FAMILY 3.1 [Cucurbita moschata])

HSP 1 Score: 995 bits (2572), Expect = 0.0
Identity = 490/580 (84.48%), Postives = 532/580 (91.72%), Query Frame = 0

Query: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60
           MEE Q+   H  RKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLT+ ANT
Sbjct: 1   MEEHQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANT 60

Query: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120
           ITNF+GTASLTPLIGAFIADSY GRFWTITVASILYQIGM  LTTSAVVP L+PPPC GG
Sbjct: 61  ITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLKPPPCKGG 120

Query: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180
           E+C +A G QLGILY SLLLTALGSGGIRPCVVSFGADQF+ESDPK+G KTYKYFNWYYF
Sbjct: 121 EICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYF 180

Query: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240
           AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TF++GY IYRHLDPSGSPFTRL
Sbjct: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRL 240

Query: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300
           LQV VAA+RKRKL+ V +  +LYQNH+IDDPISRDGKLLHT HM+FLDKAAIVTE+D++K
Sbjct: 241 LQVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVK 300

Query: 301 TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES 360
            G KPNLW LNTVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSLQQ +TM+R +T+S
Sbjct: 301 VGEKPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLTDS 360

Query: 361 FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI 420
           F+IPAGSM+VFTLLTML+T+ALYDR+F+PIARRFTGLDRGITFL+RMGIGLVIS+FATL+
Sbjct: 361 FKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFATLV 420

Query: 421 AGFVEKKRKHAA----------AGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480
           AGFVE+KRKH A          +  PIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE
Sbjct: 421 AGFVERKRKHVALIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480

Query: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITL 540
           SMRSTAMALFWTSISAGNYLSTLLVTLVHK+TAGP G NWLRNDN+N GKLENFYWL+TL
Sbjct: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLVTL 540

Query: 541 LQVFNLVYYVFCAKVYTFKPLELQGKVVDSSKVDEVQLVN 570
           LQVFNLVYY+ CAK+YTFKPLE+Q KVVDSSKVDEVQLVN
Sbjct: 541 LQVFNLVYYIVCAKMYTFKPLEVQRKVVDSSKVDEVQLVN 580

BLAST of MC04g0896 vs. NCBI nr
Match: KAG6579198.1 (Protein NRT1/ PTR FAMILY 3.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 993 bits (2568), Expect = 0.0
Identity = 490/582 (84.19%), Postives = 533/582 (91.58%), Query Frame = 0

Query: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60
           MEE+Q+   H  RKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLT+ ANT
Sbjct: 39  MEEQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANT 98

Query: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120
           ITNF+GTASLTPLIGAFIADSY GRFWTITVASILYQIGM  LTTSAVVP L+PPPC GG
Sbjct: 99  ITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLKPPPCKGG 158

Query: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180
           E+C +A G QLGILY SLLLTALGSGGIRPCVVSFGADQF+ESDPK+G KTYKYFNWYYF
Sbjct: 159 EICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYF 218

Query: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240
           AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TF++GY IYRHLDPSGSPFTRL
Sbjct: 219 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRL 278

Query: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300
           LQV VAA+RKRKL+ V +  +LYQNH+IDDPISRDGKLLHT HM+FLDKAAIVTE+D++K
Sbjct: 279 LQVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVK 338

Query: 301 TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES 360
            G KP LW LNTVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSLQQ +TM+R +T+S
Sbjct: 339 VGEKPYLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLTDS 398

Query: 361 FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI 420
           F+IPAGSM+VFTLLTML+T+ALYDR+F+PIARRFTGLDRGITFL+RMGIGLVIS+FATL+
Sbjct: 399 FKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFATLV 458

Query: 421 AGFVEKKRKHAA----------AGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480
           AGFVE+KRKH A          +  PIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE
Sbjct: 459 AGFVERKRKHVALIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 518

Query: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITL 540
           SMRSTAMALFWTSISAGNYLSTLLVTLVHK+TAGP G NWLRNDN+N GKLENFYWL+TL
Sbjct: 519 SMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLVTL 578

Query: 541 LQVFNLVYYVFCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572
           LQVFNLVYY+ CAK+YTFKPLE+Q KVVDSSKVDEVQLVN V
Sbjct: 579 LQVFNLVYYIVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 620

BLAST of MC04g0896 vs. ExPASy TrEMBL
Match: A0A6J1C163 (protein NRT1/ PTR FAMILY 3.1 OS=Momordica charantia OX=3673 GN=LOC111007508 PE=3 SV=1)

HSP 1 Score: 1140 bits (2950), Expect = 0.0
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0

Query: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60
           MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT
Sbjct: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60

Query: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120
           ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG
Sbjct: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120

Query: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180
           EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF
Sbjct: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180

Query: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240
           AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL
Sbjct: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240

Query: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300
           LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK
Sbjct: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300

Query: 301 TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES 360
           TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES
Sbjct: 301 TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES 360

Query: 361 FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI 420
           FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI
Sbjct: 361 FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI 420

Query: 421 AGFVEKKRKHAAAGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALF 480
           AGFVEKKRKHAAAGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALF
Sbjct: 421 AGFVEKKRKHAAAGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALF 480

Query: 481 WTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYV 540
           WTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYV
Sbjct: 481 WTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYV 540

Query: 541 FCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572
           FCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV
Sbjct: 541 FCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572

BLAST of MC04g0896 vs. ExPASy TrEMBL
Match: A0A6J1K1R1 (protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita maxima OX=3661 GN=LOC111489833 PE=3 SV=1)

HSP 1 Score: 998 bits (2580), Expect = 0.0
Identity = 493/582 (84.71%), Postives = 534/582 (91.75%), Query Frame = 0

Query: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60
           MEE+Q+   H  RKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLT+ ANT
Sbjct: 1   MEEQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANT 60

Query: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120
           ITNF+GTASLTPLIGAFIADSY GRFWTITVASILYQIGM  LTTSAVVP LRPPPC GG
Sbjct: 61  ITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCKGG 120

Query: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180
           E+C +A G QLGILY SLLLTALGSGGIRPCVVSFGADQF+ESDPK+G KTYKYFNWYYF
Sbjct: 121 EICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYF 180

Query: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240
           AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TF++GY IYRHLDPSGSPFTRL
Sbjct: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRL 240

Query: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300
           LQV VAA+RKRKL+ V +  +LYQNH+IDDPISRDGKLLHT HM+FLDKAAIVTE+D++K
Sbjct: 241 LQVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVK 300

Query: 301 TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES 360
            G KPNLW LNTVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSLQQ +TM+R +T+S
Sbjct: 301 VGEKPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLTDS 360

Query: 361 FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI 420
           F+IPAGSM+VFTLLTML+T+ALYDR+F+PIARRFTGLDRGITFL+RMGIGLVIS+FATL+
Sbjct: 361 FKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFATLV 420

Query: 421 AGFVEKKRKHAA----------AGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480
           AGFVE+KRKH A          +  PIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE
Sbjct: 421 AGFVERKRKHVAFIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480

Query: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITL 540
           SMRSTAMALFWTSISAGNYLSTLLVTLVHK+TAGP G NWLRNDN+N GKLENFYWL+TL
Sbjct: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLVTL 540

Query: 541 LQVFNLVYYVFCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572
           LQVFNLVYYV CAK+YTFKPLE+Q KVVDSSKVDEVQLVN V
Sbjct: 541 LQVFNLVYYVVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 582

BLAST of MC04g0896 vs. ExPASy TrEMBL
Match: A0A6J1FKQ8 (protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita moschata OX=3662 GN=LOC111445106 PE=3 SV=1)

HSP 1 Score: 995 bits (2572), Expect = 0.0
Identity = 490/580 (84.48%), Postives = 532/580 (91.72%), Query Frame = 0

Query: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60
           MEE Q+   H  RKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLT+ ANT
Sbjct: 1   MEEHQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANT 60

Query: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120
           ITNF+GTASLTPLIGAFIADSY GRFWTITVASILYQIGM  LTTSAVVP L+PPPC GG
Sbjct: 61  ITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLKPPPCKGG 120

Query: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180
           E+C +A G QLGILY SLLLTALGSGGIRPCVVSFGADQF+ESDPK+G KTYKYFNWYYF
Sbjct: 121 EICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYF 180

Query: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240
           AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TF++GY IYRHLDPSGSPFTRL
Sbjct: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRL 240

Query: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300
           LQV VAA+RKRKL+ V +  +LYQNH+IDDPISRDGKLLHT HM+FLDKAAIVTE+D++K
Sbjct: 241 LQVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVK 300

Query: 301 TGRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTES 360
            G KPNLW LNTVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSLQQ +TM+R +T+S
Sbjct: 301 VGEKPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLTDS 360

Query: 361 FQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLI 420
           F+IPAGSM+VFTLLTML+T+ALYDR+F+PIARRFTGLDRGITFL+RMGIGLVIS+FATL+
Sbjct: 361 FKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFATLV 420

Query: 421 AGFVEKKRKHAA----------AGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480
           AGFVE+KRKH A          +  PIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE
Sbjct: 421 AGFVERKRKHVALIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPE 480

Query: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITL 540
           SMRSTAMALFWTSISAGNYLSTLLVTLVHK+TAGP G NWLRNDN+N GKLENFYWL+TL
Sbjct: 481 SMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLVTL 540

Query: 541 LQVFNLVYYVFCAKVYTFKPLELQGKVVDSSKVDEVQLVN 570
           LQVFNLVYY+ CAK+YTFKPLE+Q KVVDSSKVDEVQLVN
Sbjct: 541 LQVFNLVYYIVCAKMYTFKPLEVQRKVVDSSKVDEVQLVN 580

BLAST of MC04g0896 vs. ExPASy TrEMBL
Match: A0A1S3BD88 (protein NRT1/ PTR FAMILY 3.1 OS=Cucumis melo OX=3656 GN=LOC103488612 PE=3 SV=1)

HSP 1 Score: 974 bits (2519), Expect = 0.0
Identity = 489/585 (83.59%), Postives = 527/585 (90.09%), Query Frame = 0

Query: 1   MEEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANT 60
           ME E+T+ KH  RKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMP T+AANT
Sbjct: 1   MEMEKTE-KHMERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPSTKAANT 60

Query: 61  ITNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGG 120
           ITNFSGTASLTPLIGAFIADSY GRFWTITVASILYQIGM  LTTSAVVP LRPPPC  G
Sbjct: 61  ITNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCKAG 120

Query: 121 EVCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYF 180
           E+C +A  GQLGILY+SLLLTALGSGGIRPCVVSFGADQFEE DPKRG KTYKYFNWYYF
Sbjct: 121 EICKEATEGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYF 180

Query: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRL 240
           AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAM +SI+TF+VGYPIYRHLDPSGSPF+RL
Sbjct: 181 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLVSIITFVVGYPIYRHLDPSGSPFSRL 240

Query: 241 LQVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELK 300
           LQVGVAAYRKRK+  VSD  +LYQN +ID PISRDG LLHTKHM+FLDKAA+VTEE+++K
Sbjct: 241 LQVGVAAYRKRKVGSVSDSNLLYQNRDIDGPISRDGVLLHTKHMRFLDKAAVVTEEEKVK 300

Query: 301 TGR---KPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHV 360
            G    KPNLWKL+TVHRVEELKSVIRMGPIWA+GIILITAYSQQNTFSL QASTMNRH+
Sbjct: 301 MGEELGKPNLWKLSTVHRVEELKSVIRMGPIWAAGIILITAYSQQNTFSLLQASTMNRHL 360

Query: 361 TESFQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFA 420
           T SFQIPAGSMTVFTLLTML+TVALYDRVF+PIAR+FTGLDRGITFL RMGIGLVIS+FA
Sbjct: 361 TNSFQIPAGSMTVFTLLTMLITVALYDRVFIPIARKFTGLDRGITFLTRMGIGLVISIFA 420

Query: 421 TLIAGFVEKKRKHAAAGD----------PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQ 480
           TLIAGFVE+KRKH A             PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQ
Sbjct: 421 TLIAGFVEQKRKHVAFNHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQ 480

Query: 481 SPESMRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWL 540
           SPESMRSTAMALFWTSISAG+YLSTLLV+LVHK++ G  G NW+RNDN+N GKLENFYWL
Sbjct: 481 SPESMRSTAMALFWTSISAGSYLSTLLVSLVHKYSGGANGENWIRNDNINKGKLENFYWL 540

Query: 541 ITLLQVFNLVYYVFCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572
           IT+LQV NL YY+ CA+ YTFKPLE+Q KV+D SKVDE+QLVN V
Sbjct: 541 ITVLQVLNLAYYIVCARFYTFKPLEVQRKVMDGSKVDELQLVNPV 584

BLAST of MC04g0896 vs. ExPASy TrEMBL
Match: A0A0A0K953 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G037500 PE=3 SV=1)

HSP 1 Score: 948 bits (2450), Expect = 0.0
Identity = 476/567 (83.95%), Postives = 510/567 (89.95%), Query Frame = 0

Query: 19  ITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTITNFSGTASLTPLIGAFI 78
           +T     +NEICEKIAVVGFNTNMISYLTKQLHMP T+AANTITNFSGTASLTPLIGAFI
Sbjct: 4   VTPSLSLSNEICEKIAVVGFNTNMISYLTKQLHMPSTKAANTITNFSGTASLTPLIGAFI 63

Query: 79  ADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGEVCSQADGGQLGILYISL 138
           ADSY GRFWTITVASILYQIGM  LTTSAVVP LRPPPC  GE+C +A GGQLGILY+SL
Sbjct: 64  ADSYVGRFWTITVASILYQIGMVSLTTSAVVPGLRPPPCKAGEICKEATGGQLGILYVSL 123

Query: 139 LLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAMGASMLVAVTVLVYVQD 198
           LLTALGSGGIRPCVVSFGADQFEE DPKRG KTYKYFNWYYFAMGASMLVAVTVLVYVQD
Sbjct: 124 LLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVAVTVLVYVQD 183

Query: 199 NIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQVGVAAYRKRKLDPVSD 258
           NIGWGWGFGIPTIAM +SI+TF+VGYPIYRHLDPSGSPFTRLLQVGVAAY+KRK+  VSD
Sbjct: 184 NIGWGWGFGIPTIAMLVSIITFVVGYPIYRHLDPSGSPFTRLLQVGVAAYKKRKVCIVSD 243

Query: 259 YKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTGR---KPNLWKLNTVHR 318
             +LYQN +IDDPISRDG LLHTKHM+FLDKAAIVTEE+++K G    KPNLWKL+TVHR
Sbjct: 244 SNLLYQNRDIDDPISRDGNLLHTKHMRFLDKAAIVTEEEKVKMGEELGKPNLWKLSTVHR 303

Query: 319 VEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESFQIPAGSMTVFTLLT 378
           VEELKSVIRMGPIWASGIILITAYSQQ TFSL QASTMNRH+T SFQIPAGSMTVFTLLT
Sbjct: 304 VEELKSVIRMGPIWASGIILITAYSQQYTFSLLQASTMNRHLTNSFQIPAGSMTVFTLLT 363

Query: 379 MLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIAGFVEKKRKHAAAGD 438
           ML+TVALYDRVF+PIARRFTGLDRGITFL RMGIGLVIS+FATLIAGFVE+KRKH A   
Sbjct: 364 MLITVALYDRVFIPIARRFTGLDRGITFLTRMGIGLVISIFATLIAGFVEQKRKHVAFTH 423

Query: 439 ----------PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSIS 498
                     PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSIS
Sbjct: 424 GLIDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSIS 483

Query: 499 AGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYVFCAKV 558
           AGNYLSTLLV+LVHK+++G  G NW+RNDN++ GKLENFYWL+T LQV NL YY+ CAK 
Sbjct: 484 AGNYLSTLLVSLVHKYSSGANGENWIRNDNISKGKLENFYWLVTGLQVLNLAYYIVCAKF 543

Query: 559 YTFKPLELQGKVVDSSKVDEVQLVNHV 572
           YTFKPLE+Q KVVDSSKVDE+QLVN V
Sbjct: 544 YTFKPLEVQRKVVDSSKVDELQLVNPV 570

BLAST of MC04g0896 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 839.0 bits (2166), Expect = 2.3e-243
Identity = 415/580 (71.55%), Postives = 485/580 (83.62%), Query Frame = 0

Query: 2   EEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTI 61
           EEE+  +    R KGGLITMPFIFANEICEK+AVVGF+ NMISYLT QLH+PLT+AANT+
Sbjct: 11  EEEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTL 70

Query: 62  TNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGE 121
           TNF+GT+SLTPL+GAFIADS+AGRFWTIT ASI+YQIGMT+LT SA++PTLRPPPC G E
Sbjct: 71  TNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEE 130

Query: 122 VCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFA 181
           VC  AD  QL ILY++LLL ALGSGGIRPCVV+FGADQF+ESDP +  KT+ YFNWYYF 
Sbjct: 131 VCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFC 190

Query: 182 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLL 241
           MGA++L+AVTVLV++QDN+GWG G GIPT+AMFLS++ F+ G+ +YRHL P+GSPFTRL+
Sbjct: 191 MGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLI 250

Query: 242 QVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKT 301
           QVGVAA+RKRKL  VSD  +LY N EID PIS  GKL HTKHM FLDKAAIVTEED LK 
Sbjct: 251 QVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIVTEEDNLKP 310

Query: 302 GRKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESF 361
           G+ PN W+L+TVHRVEELKSVIRMGPI ASGI+LITAY+QQ TFSLQQA TMNRH+T SF
Sbjct: 311 GQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSF 370

Query: 362 QIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIA 421
           QIPAGSM+VFT + ML T+  YDRVFV +AR+FTGL+RGITFL RMGIG VIS+ ATL+A
Sbjct: 371 QIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVA 430

Query: 422 GFVEKKRKHAAAGD----------PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPES 481
           GFVE KRK  A             PIS  WL+PQY LHG+AEAFMSIGHLEFFYDQ+PES
Sbjct: 431 GFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPES 490

Query: 482 MRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLL 541
           MRSTA ALFW +IS GNY+STLLVTLVHKF+A P GSNWL ++NLN G+LE FYWLIT+L
Sbjct: 491 MRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVL 550

Query: 542 QVFNLVYYVFCAKVYTFKPLELQGKVVDSSKV-DEVQLVN 571
           Q  NLVYY++CAK+YT+KP+++     DSS V +E+QL N
Sbjct: 551 QAVNLVYYLWCAKIYTYKPVQVHHSKEDSSPVKEELQLSN 590

BLAST of MC04g0896 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 486.1 bits (1250), Expect = 3.8e-137
Identity = 247/551 (44.83%), Postives = 359/551 (65.15%), Query Frame = 0

Query: 7   KNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTITNFSG 66
           KN     K G      FI  NE CE++A  G  TN+++YL  +L+     AAN +TN+SG
Sbjct: 17  KNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSG 76

Query: 67  TASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGEVCSQA 126
           T  +TPLIGAFIAD+Y GR+WTI     +Y  GMT+LT SA VP L+P  CN  + C   
Sbjct: 77  TCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN-ADTC-HP 136

Query: 127 DGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAMGASM 186
           +  Q  + +++L + ALG+GGI+PCV SFGADQF+E+D    +K   +FNW+YF++    
Sbjct: 137 NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGA 196

Query: 187 LVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQVGVA 246
           L+A TVLV++Q N+GWGWGFG+PT+AM +++  F  G   YR   P GSP TR+ QV VA
Sbjct: 197 LIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVA 256

Query: 247 AYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTGRKPN 306
           A+RK  +    D  +L++  + +  I    KL+HT ++KF DKAA+ ++ D +K G + N
Sbjct: 257 AFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDG-EVN 316

Query: 307 LWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESFQIPAG 366
            W+L +V +VEELKS+I + P+WA+GI+  T YSQ +T  + Q +TM++H+ ++F+IP+ 
Sbjct: 317 PWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSA 376

Query: 367 SMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIAGFVEK 426
           S+++F  +++L    +YD+  +P+AR+FT  +RG T L+RMGIGLV+S+FA + AG +E 
Sbjct: 377 SLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEV 436

Query: 427 KR-----KHAAAGDP---ISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMA 486
            R      H A       +S+FW +PQY L G AE F  IG LEFFYDQ+P++MRS   A
Sbjct: 437 VRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSA 496

Query: 487 LFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVY 546
           L  T+++ GNYLST+LVT+V K T   G   W+  DNLN G L+ F++L+  L   N + 
Sbjct: 497 LSLTTVALGNYLSTVLVTVVMKITKKNGKPGWI-PDNLNRGHLDYFFYLLATLSFLNFLV 556

Query: 547 YVFCAKVYTFK 550
           Y++ +K Y +K
Sbjct: 557 YLWISKRYKYK 563

BLAST of MC04g0896 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 486.1 bits (1250), Expect = 3.8e-137
Identity = 255/535 (47.66%), Postives = 354/535 (66.17%), Query Frame = 0

Query: 23  FIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTITNFSGTASLTPLIGAFIADSY 82
           FI   E CE++A  G +TN+I+YL KQ++M    A+ +++N+SGT   TPLIGAFIAD+Y
Sbjct: 34  FILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSNWSGTCYATPLIGAFIADAY 93

Query: 83  AGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGEVCSQADGGQLGILYISLLLTA 142
            GR+WTI    ++Y  GMT+LT SA VP L  P C+ GE C  A  GQ  I +I+L L A
Sbjct: 94  LGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTCS-GETC-HATAGQTAITFIALYLIA 153

Query: 143 LGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAMGASMLVAVTVLVYVQDNIGW 202
           LG+GGI+PCV SFGADQF+++D K       +FNW+YF +    ++A +VLV++Q N+GW
Sbjct: 154 LGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGW 213

Query: 203 GWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQVGVAAYRKRKLDPVSDYKML 262
           GWG G+PT+AM +++V F  G   YR   P GSP TR+LQV VA+ RK K+    D  +L
Sbjct: 214 GWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVASCRKSKVKIPEDESLL 273

Query: 263 YQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTGRKPNLWKLNTVHRVEELKSV 322
           Y+N + +  I    KL HTK + F DKAA+ TE D  K   K + WKL TV +VEELK++
Sbjct: 274 YENQDAESSIIGSRKLEHTKILTFFDKAAVETESDN-KGAAKSSSWKLCTVTQVEELKAL 333

Query: 323 IRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESFQIPAGSMTVFTLLTMLVTVAL 382
           IR+ PIWA+GI+  + YSQ  T  + Q +T+++H+  +F+IP+ S+++F  L++L    +
Sbjct: 334 IRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPV 393

Query: 383 YDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIAGFVEKKR-----KHAAAGD-- 442
           YD++ VP AR++TG +RG T L+R+GIGLVIS+F+ + AG +E  R      H    +  
Sbjct: 394 YDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEVARLNYVQTHNLYNEET 453

Query: 443 -PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSISAGNYLSTLL 502
            P+++FW VPQY L G AE F  IG LEFFYDQ+P++MRS   AL  T+I+ GNYLST L
Sbjct: 454 IPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFL 513

Query: 503 VTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYVFCAKVYTFK 550
           VTLV K T   G   W+   NLN G L+ F+WL+  L   N + Y++ AK YT+K
Sbjct: 514 VTLVTKVTRSGGRPGWIAK-NLNNGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYK 563

BLAST of MC04g0896 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 477.6 bits (1228), Expect = 1.4e-134
Identity = 254/541 (46.95%), Postives = 349/541 (64.51%), Query Frame = 0

Query: 8   NKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTITNFSGT 67
           N  + +K G     PFI  NE CE++A  G   N+I+Y T +LH     AA+ +  + GT
Sbjct: 46  NPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGT 105

Query: 68  ASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNG--GEVCSQ 127
             +TPLIGA IADSY GR+WTI   S +Y IGM +LT SA +P L+P  C G    +CS 
Sbjct: 106 CYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCSP 165

Query: 128 ADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAMGAS 187
           A   Q  + +  L L ALG+GGI+PCV SFGADQF+++DP+  ++   +FNW+YF++   
Sbjct: 166 ATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIG 225

Query: 188 MLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQVGV 247
             ++ T+LV+VQ+N+GWG GF IPT+ M +SI +F +G P+YR   P GSP TR+ QV V
Sbjct: 226 SFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLV 285

Query: 248 AAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTGRKP 307
           AAYRK KL+   D   LY+  E +  I+   K+ HT   KFLDKAA+++E  E K+G   
Sbjct: 286 AAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEY-ESKSGAFS 345

Query: 308 NLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESFQIPA 367
           N WKL TV +VEE+K++IRM PIWASGI+    YSQ +T  +QQ  +MNR +  SF+IP 
Sbjct: 346 NPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNR-IIRSFEIPP 405

Query: 368 GSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIAGFVE 427
            S  VF  L +L+++ +YDR  VP  RRFTG+ +G+T L+RMGIGL +SV +   A  VE
Sbjct: 406 ASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIVE 465

Query: 428 KKRKHAAAG-DPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSI 487
             R   A     +S+FW +PQY L G+AE F  IG +EFFYD+SP++MRS   AL   + 
Sbjct: 466 TVRLQLAQDFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSVCSALALLNT 525

Query: 488 SAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNL-VYYVFCA 545
           + G+YLS+L++TLV  FTA  G   W+  D+LN G L+ F+WL+  L + N+ VY + C 
Sbjct: 526 AVGSYLSSLILTLVAYFTALGGKDGWV-PDDLNKGHLDYFFWLLVSLGLVNIPVYALICV 583

BLAST of MC04g0896 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 470.7 bits (1210), Expect = 1.7e-132
Identity = 252/545 (46.24%), Postives = 343/545 (62.94%), Query Frame = 0

Query: 14  KKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTITNFSGTASLTPL 73
           K G     PFI  NE CE++A  G   N+I+YLT +LH     AA  +T + GT  LTPL
Sbjct: 41  KTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLTPL 100

Query: 74  IGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGEVCSQADGGQLGI 133
           IGA +AD+Y GR+WTI   S +Y IGM+ LT SA VP L+P  C  G+ C  A   Q  +
Sbjct: 101 IGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECI-GDFCPSATPAQYAM 160

Query: 134 LYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAMGASMLVAVTVL 193
            +  L L ALG+GGI+PCV SFGADQF+++D +  ++   +FNW+YF++    LV+ ++L
Sbjct: 161 FFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSSSLL 220

Query: 194 VYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQVGVAAYRKRKL 253
           V++Q+N GWG GFGIPT+ M L+I +F  G P+YR   P GSP TR+ QV VA++RK  +
Sbjct: 221 VWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRKSSV 280

Query: 254 DPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTGRKPNLWKLNTV 313
               D  +LY+  + +  I+   K+ HT   ++LDKAA+++EE E K+G   N W+L TV
Sbjct: 281 KVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEE-ESKSGDYSNSWRLCTV 340

Query: 314 HRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTESFQIPAGSMTVFTL 373
            +VEELK +IRM PIWASGII    Y+Q +T  +QQ   MN  +  SFQ+P  ++  F  
Sbjct: 341 TQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKI-GSFQLPPAALGTFDT 400

Query: 374 LTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATLIAGFVEKKRKHAA- 433
            ++++ V LYDR  VP+AR+FTG+D+G T ++RMGIGL +SV     A  VE  R H A 
Sbjct: 401 ASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMAN 460

Query: 434 --------AGDPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSI 493
                   A  PISV W +PQY + G AE F  IG LEFFYDQSP++MRS   AL   + 
Sbjct: 461 DLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTN 520

Query: 494 SAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLITLLQVFNLVYYVFCAK 550
           + GNYLS+L++TLV  FT   G   W+ +DNLN G L+ F+WL+  L + N+  Y F A 
Sbjct: 521 ALGNYLSSLILTLVTYFTTRNGQEGWI-SDNLNSGHLDYFFWLLAGLSLVNMAVYFFSAA 580

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SX203.3e-24271.55Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Q9M3905.4e-13644.83Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB85.4e-13647.66Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Q93Z201.9e-13346.95Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
P460322.4e-13146.24Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022135586.10.0100.00protein NRT1/ PTR FAMILY 3.1 [Momordica charantia][more]
XP_022994014.10.084.71protein NRT1/ PTR FAMILY 3.1 [Cucurbita maxima][more]
XP_023550855.10.084.19protein NRT1/ PTR FAMILY 3.1 [Cucurbita pepo subsp. pepo][more]
XP_022939103.10.084.48protein NRT1/ PTR FAMILY 3.1 [Cucurbita moschata][more]
KAG6579198.10.084.19Protein NRT1/ PTR FAMILY 3.1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1C1630.0100.00protein NRT1/ PTR FAMILY 3.1 OS=Momordica charantia OX=3673 GN=LOC111007508 PE=3... [more]
A0A6J1K1R10.084.71protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita maxima OX=3661 GN=LOC111489833 PE=3 SV... [more]
A0A6J1FKQ80.084.48protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita moschata OX=3662 GN=LOC111445106 PE=3 ... [more]
A0A1S3BD880.083.59protein NRT1/ PTR FAMILY 3.1 OS=Cucumis melo OX=3656 GN=LOC103488612 PE=3 SV=1[more]
A0A0A0K9530.083.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G037500 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G68570.12.3e-24371.55Major facilitator superfamily protein [more]
AT3G54140.13.8e-13744.83peptide transporter 1 [more]
AT5G01180.13.8e-13747.66peptide transporter 5 [more]
AT1G62200.11.4e-13446.95Major facilitator superfamily protein [more]
AT2G02040.11.7e-13246.24peptide transporter 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 86..509
e-value: 1.0E-104
score: 350.5
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 11..557
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 2..554
e-value: 4.2E-187
score: 624.5
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 16..544
NoneNo IPR availablePANTHERPTHR11654:SF544BNAA07G24560D PROTEINcoord: 11..557

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0896.1MC04g0896.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010336 gibberellic acid homeostasis
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0097708 intracellular vesicle
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity