MC04g0681 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0681
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	caffeic acid 3-O-methyltransferase-like
Location	MC04: 6422943 .. 6423653 (+)
RNA-Seq Expression	MC04g0681
Synteny	MC04g0681

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTGTAGAACATATAGGAGGTGACATGTTTCAGAGCGTCCAAAGGGAGATTCTATTTTCATGAAGGTAGATTTATTGGTCTTAATTAATTCTACCTTGTAGAAACATATTTGTATGATAAATATTTACCATCTATAAAAAATGTTCTTGATATTGTATAGACTTGAGGAAAAAAAATTTATTGTTTCTGGAATTTTCTTCAATTATAATACTCACATAAAATTTAATTCTAACTTTAAATTTTGGTACTAAATTTGAATAAGTTGTAAAATTAAGTAGTCTTGTGATTAGACTAATGTATTTTCATGATTGGGCTTAAGTATTTATGTAACTTGATTTGCTGGAATTTGTGTCATTTATTGATTAATATATTATAATTTTTTTGTTTCCATAAAATTGTTGTAGTGGATCCTCCATGATTGGAGTGATGAGCACCGTGTGAAATTGTTGAAGAATTGTCATGCTGCGATTCCAGATGATGGAAAGATAATTGTTGTAGAGGTCGTACTTCCAATGGTGGTTGAGACCACGAGTGCAGTGAAATCCGTCGCTAAATCCGTCGCTCAAGATGATGTACTTATGATGACTCAAAATCCTGGGGGAAAAGAGCGAACTTGAGATGAATTCAAAGCCCTTGCTAACTGTAGCTGGGTTTAAATGCATCAAATTTGAATGTTTTGTATGTAATCTTTGGATTATGGAGTTCTTCAAA

mRNA sequence

GGTGTAGAACATATAGGAGGTGACATGTTTCAGAGCCCAAAGGGAGATTCTATTTTCATGAAGTGGATCCTCCATGATTGGAGTGATGAGCACCGTGTGAAATTGTTGAAGAATTGTCATGCTGCGATTCCAGATGATGGAAAGATAATTGTTGTAGAGGTCGTACTTCCAATGGTGGTTGAGACCACGAGTGCAGTGAAATCCGTCGCTAAATCCGTCGCTCAAGATGATGTACTTATGATGACTCAAAATCCTGGGGGAAAAGAGCGAGAGATGAATTCAAAGCCCTTGCTAACTGTAGCTGGGTTTAAATGCATCAAATTTGAATGTTTTGTATGTAATCTTTGGATTATGGAGTTCTTCAAA

Coding sequence (CDS)

GGTGTAGAACATATAGGAGGTGACATGTTTCAGAGCCCAAAGGGAGATTCTATTTTCATGAAGTGGATCCTCCATGATTGGAGTGATGAGCACCGTGTGAAATTGTTGAAGAATTGTCATGCTGCGATTCCAGATGATGGAAAGATAATTGTTGTAGAGGTCGTACTTCCAATGGTGGTTGAGACCACGAGTGCAGTGAAATCCGTCGCTAAATCCGTCGCTCAAGATGATGTACTTATGATGACTCAAAATCCTGGGGGAAAAGAGCGAGAGATGAATTCAAAGCCCTTGCTAACTGTAGCTGGGTTTAAATGCATCAAATTTGAATGTTTTGTATGTAATCTTTGGATTATGGAGTTCTTCAAA

Protein sequence

GVEHIGGDMFQSPKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMVVETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNSKPLLTVAGFKCIKFECFVCNLWIMEFFK

Homology

[Close]

BLAST of MC04g0681 vs. ExPASy Swiss-Prot
Match: Q9XGW0 (Caffeic acid 3-O-methyltransferase 1 OS=Ocimum basilicum OX=39350 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 7.2e-30
Identity = 69/124 (55.65%), Postives = 81/124 (65.32%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+GGDMF S PKGD+IFMKWI HDWSDEH VK LKNC+ A+P +GK+I+ E VLP  
Sbjct: 242 GVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYDALPQNGKVILAECVLPEA 301

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKER-EMNSKPLLTVAGFKCIKFECFVCNLWIM 120
            +T  A     K+V   DV+M+  NPGGKER E   + L   AGFK     C   N WIM
Sbjct: 302 PDTGLA----TKNVVHIDVIMLAHNPGGKERTEKEFQGLAKAAGFKQFNKACCAYNTWIM 361

Query: 121 EFFK 123
           E  K
Sbjct: 362 ELLK 361

[Close]

BLAST of MC04g0681 vs. ExPASy Swiss-Prot
Match: O23760 (Caffeic acid 3-O-methyltransferase OS=Clarkia breweri OX=36903 GN=COMT PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 9.4e-30
Identity = 67/124 (54.03%), Postives = 83/124 (66.94%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+GGDMF S PKGD+IFMKWI HDWSDEH +K LKNC+AA+P+ GK+IV E +LP+ 
Sbjct: 249 GVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPEHGKVIVAECILPLS 308

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKER-EMNSKPLLTVAGFKCIKFECFVCNLWIM 120
            + + A     K V   D +M+  NPGGKER E   + L   AGFK  K  C   N ++M
Sbjct: 309 PDPSLA----TKGVIHIDAIMLAHNPGGKERTEKEFEALAIGAGFKGFKVACCAFNTYVM 368

Query: 121 EFFK 123
           EF K
Sbjct: 369 EFLK 368

[Close]

BLAST of MC04g0681 vs. ExPASy Swiss-Prot
Match: Q8GU25 (Caffeic acid 3-O-methyltransferase OS=Rosa chinensis OX=74649 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.6e-29
Identity = 65/124 (52.42%), Postives = 87/124 (70.16%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GV+H+GGDMF S PKGD+IFMKWI HDWSDEH +K LKNC+AA+PD+GK+I+ E +LP+ 
Sbjct: 244 GVQHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPDNGKVILGECILPVA 303

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKER-EMNSKPLLTVAGFKCIKFECFVCNLWIM 120
            +T+ A     K V   DV+M+  NPGGKER E   + L   +GF+ I+  C   N +++
Sbjct: 304 PDTSLA----TKGVVHIDVVMLAHNPGGKERTEQEFEALAKGSGFQGIRVACNAFNTYVI 363

Query: 121 EFFK 123
           EF K
Sbjct: 364 EFLK 363

[Close]

BLAST of MC04g0681 vs. ExPASy Swiss-Prot
Match: Q8LL87 (Caffeic acid 3-O-methyltransferase OS=Coffea canephora OX=49390 GN=COMT PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.1e-29
Identity = 68/124 (54.84%), Postives = 84/124 (67.74%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+GGDMF S PKGD+IFMKWI HDWSD+H  KLLKNC+ A+PD+GK+I+ E VLP  
Sbjct: 231 GVEHVGGDMFVSVPKGDAIFMKWICHDWSDDHCRKLLKNCYQALPDNGKVILAECVLPEA 290

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKER-EMNSKPLLTVAGFKCIKFECFVCNLWIM 120
            +T+ A     ++V   DV+M+  NPGGKER E   + L   AGFK  +  C   N WIM
Sbjct: 291 PDTSLA----TQNVVHVDVVMLAHNPGGKERTEKEFEALAKGAGFKEFRKVCSAVNTWIM 350

Query: 121 EFFK 123
           E  K
Sbjct: 351 ELCK 350

[Close]

BLAST of MC04g0681 vs. ExPASy Swiss-Prot
Match: P28002 (Caffeic acid 3-O-methyltransferase OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 2.7e-29
Identity = 65/124 (52.42%), Postives = 84/124 (67.74%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+GGDMF S PK D++FMKWI HDWSDEH +K LKNC+ A+PD+GK+IV E +LP+ 
Sbjct: 244 GVEHVGGDMFVSIPKADAVFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVA 303

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKER-EMNSKPLLTVAGFKCIKFECFVCNLWIM 120
            +++ A     K V   DV+M+  NPGGKER +   + L   AGF+  K  C   N +IM
Sbjct: 304 PDSSLA----TKGVVHIDVIMLAHNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNTYIM 363

Query: 121 EFFK 123
           EF K
Sbjct: 364 EFLK 363

[Close]

BLAST of MC04g0681 vs. NCBI nr
Match: XP_023540486.1 (caffeic acid 3-O-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 173 bits (438), Expect = 4.07e-50
Identity = 86/124 (69.35%), Postives = 101/124 (81.45%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+GGDMF+  PKGD+IFMKWILHDWSDEH VKLLKNC+AAIPDDGK+IV+E V+P V
Sbjct: 250 GVEHVGGDMFEKVPKGDAIFMKWILHDWSDEHCVKLLKNCYAAIPDDGKVIVMEGVVPTV 309

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNS-KPLLTVAGFKCIKFECFVCNLWIM 120
            ETT+A    AK++ Q D+LMMTQNPGGKER  +  K L T AGFK +KF CF+CN WI+
Sbjct: 310 PETTAA----AKAIGQGDMLMMTQNPGGKERTRDEFKALATKAGFKHVKFVCFICNFWII 369

Query: 121 EFFK 122
           EF K
Sbjct: 370 EFLK 369

[Close]

BLAST of MC04g0681 vs. NCBI nr
Match: XP_022143497.1 (caffeic acid 3-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 171 bits (432), Expect = 2.47e-49
Identity = 87/124 (70.16%), Postives = 100/124 (80.65%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEHIGGDMF+S PKGD+IFMKWILHDWSD+H VKLLK+CH AIPD+GK+IVV+ V+PMV
Sbjct: 239 GVEHIGGDMFESVPKGDAIFMKWILHDWSDKHCVKLLKBCHVAIPDEGKVIVVDAVVPMV 298

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNS-KPLLTVAGFKCIKFECFVCNLWIM 120
           +ETTS +K V     Q DVLMMTQ+PGGKER  +  K L T A FK I FECFV NLW+M
Sbjct: 299 IETTSTMKFVT----QGDVLMMTQSPGGKERTRDEFKALATKARFKHINFECFVYNLWVM 358

Query: 121 EFFK 122
           EF K
Sbjct: 359 EFIK 358

[Close]

BLAST of MC04g0681 vs. NCBI nr
Match: XP_022143528.1 (caffeic acid 3-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 171 bits (432), Expect = 2.78e-49
Identity = 86/124 (69.35%), Postives = 100/124 (80.65%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEHIGGDMF+S PKGD+IFMKWILHDWSDEH VKLLKNC+AAIP+DGK+I V+ V+P+V
Sbjct: 244 GVEHIGGDMFESVPKGDAIFMKWILHDWSDEHCVKLLKNCYAAIPNDGKVIAVDAVVPLV 303

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNS-KPLLTVAGFKCIKFECFVCNLWIM 120
           +ETT  +KS+     Q DVLMMTQNPGGKER  +  K L T  GFK I FECFV NLW++
Sbjct: 304 IETTITMKSIT----QGDVLMMTQNPGGKERTRDEFKALATKTGFKRINFECFVYNLWVI 363

Query: 121 EFFK 122
           EF K
Sbjct: 364 EFIK 363

[Close]

BLAST of MC04g0681 vs. NCBI nr
Match: XP_011468886.1 (PREDICTED: LOW QUALITY PROTEIN: caffeic acid 3-O-methyltransferase-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 169 bits (429), Expect = 7.46e-49
Identity = 86/124 (69.35%), Postives = 101/124 (81.45%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+GGDMF S P GD+IFMKWILHDWSDEH +KLLKNC+ AIPD+GK+IVVE +LP+V
Sbjct: 242 GVEHVGGDMFASVPSGDAIFMKWILHDWSDEHCLKLLKNCYNAIPDNGKVIVVEALLPVV 301

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKER-EMNSKPLLTVAGFKCIKFECFVCNLWIM 120
            ET++AVKS +    Q DVLMMTQNPGGKER E     L T AGF+ I++ECFVCN W+M
Sbjct: 302 PETSTAVKSTS----QIDVLMMTQNPGGKERTEQEFIALATGAGFRGIRYECFVCNFWVM 361

Query: 121 EFFK 122
           EFFK
Sbjct: 362 EFFK 361

[Close]

BLAST of MC04g0681 vs. NCBI nr
Match: PNX97772.1 (caffeic acid 3-O-methyltransferase-like protein [Trifolium pratense])

HSP 1 Score: 162 bits (409), Expect = 9.02e-49
Identity = 82/124 (66.13%), Postives = 98/124 (79.03%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+GGDMF+S PKGD+IFMKWILHDWSDEH +KLLKNC+ AIP+DGK+IV+E ++P+V
Sbjct: 4   GVEHVGGDMFESVPKGDAIFMKWILHDWSDEHCLKLLKNCYDAIPNDGKVIVLEALIPIV 63

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNSK-PLLTVAGFKCIKFECFVCNLWIM 120
            E +     VAKS++Q DVLMMTQNPGGKER       L T  GF  I+FECFV N W+M
Sbjct: 64  PENSH----VAKSISQLDVLMMTQNPGGKERSKQEFIDLATKVGFSGIRFECFVRNFWVM 123

Query: 121 EFFK 122
           EFFK
Sbjct: 124 EFFK 123

[Close]

BLAST of MC04g0681 vs. ExPASy TrEMBL
Match: A0A6J1CQX8 (caffeic acid 3-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111013400 PE=3 SV=1)

HSP 1 Score: 171 bits (432), Expect = 1.35e-49
Identity = 86/124 (69.35%), Postives = 100/124 (80.65%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEHIGGDMF+S PKGD+IFMKWILHDWSDEH VKLLKNC+AAIP+DGK+I V+ V+P+V
Sbjct: 244 GVEHIGGDMFESVPKGDAIFMKWILHDWSDEHCVKLLKNCYAAIPNDGKVIAVDAVVPLV 303

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNS-KPLLTVAGFKCIKFECFVCNLWIM 120
           +ETT  +KS+     Q DVLMMTQNPGGKER  +  K L T  GFK I FECFV NLW++
Sbjct: 304 IETTITMKSIT----QGDVLMMTQNPGGKERTRDEFKALATKTGFKRINFECFVYNLWVI 363

Query: 121 EFFK 122
           EF K
Sbjct: 364 EFIK 363

[Close]

BLAST of MC04g0681 vs. ExPASy TrEMBL
Match: A0A6J1CQZ7 (caffeic acid 3-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111013374 PE=3 SV=1)

HSP 1 Score: 169 bits (429), Expect = 3.36e-49
Identity = 87/124 (70.16%), Postives = 100/124 (80.65%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEHIGGDMF+S PKGD+IFMKWILHDWSD+H VKLLK+CH AIPD+GK+IVV+ V+PMV
Sbjct: 239 GVEHIGGDMFESVPKGDAIFMKWILHDWSDKHCVKLLKDCHVAIPDEGKVIVVDAVVPMV 298

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNS-KPLLTVAGFKCIKFECFVCNLWIM 120
           +ETTS +K V     Q DVLMMTQ+PGGKER  +  K L T A FK I FECFV NLW+M
Sbjct: 299 IETTSTMKFVT----QGDVLMMTQSPGGKERTRDEFKALATKARFKHINFECFVYNLWVM 358

Query: 121 EFFK 122
           EF K
Sbjct: 359 EFIK 358

[Close]

BLAST of MC04g0681 vs. ExPASy TrEMBL
Match: A0A2K3N417 (Caffeic acid 3-O-methyltransferase-like protein OS=Trifolium pratense OX=57577 GN=L195_g021006 PE=3 SV=1)

HSP 1 Score: 162 bits (409), Expect = 4.37e-49
Identity = 82/124 (66.13%), Postives = 98/124 (79.03%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+GGDMF+S PKGD+IFMKWILHDWSDEH +KLLKNC+ AIP+DGK+IV+E ++P+V
Sbjct: 4   GVEHVGGDMFESVPKGDAIFMKWILHDWSDEHCLKLLKNCYDAIPNDGKVIVLEALIPIV 63

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNSK-PLLTVAGFKCIKFECFVCNLWIM 120
            E +     VAKS++Q DVLMMTQNPGGKER       L T  GF  I+FECFV N W+M
Sbjct: 64  PENSH----VAKSISQLDVLMMTQNPGGKERSKQEFIDLATKVGFSGIRFECFVRNFWVM 123

Query: 121 EFFK 122
           EFFK
Sbjct: 124 EFFK 123

[Close]

BLAST of MC04g0681 vs. ExPASy TrEMBL
Match: A0A2P6SIY5 (Putative caffeate O-methyltransferase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0361221 PE=3 SV=1)

HSP 1 Score: 168 bits (426), Expect = 1.26e-48
Identity = 85/124 (68.55%), Postives = 101/124 (81.45%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+GGDMF S P GD+IFMKWILHDWSDEH +KLLKNC+ AIPD+GK+IVVE +LP+V
Sbjct: 251 GVEHVGGDMFASVPSGDAIFMKWILHDWSDEHCLKLLKNCYNAIPDNGKVIVVEALLPVV 310

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKER-EMNSKPLLTVAGFKCIKFECFVCNLWIM 120
            ET++AVKS +    Q DVLMMTQNPGGKER E     L T AGF+ I++ECFVCN W++
Sbjct: 311 PETSTAVKSTS----QLDVLMMTQNPGGKERSEEEFIALATAAGFRGIRYECFVCNFWVL 370

Query: 121 EFFK 122
           EFFK
Sbjct: 371 EFFK 370

[Close]

BLAST of MC04g0681 vs. ExPASy TrEMBL
Match: A0A6J1FBC5 (caffeic acid 3-O-methyltransferase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111443875 PE=3 SV=1)

HSP 1 Score: 168 bits (425), Expect = 1.73e-48
Identity = 84/124 (67.74%), Postives = 100/124 (80.65%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+GGDMF+  PKGD+IFMKWILHDWSDEH VKLLKNC++AIPDDGK+IV+E V+P V
Sbjct: 250 GVEHVGGDMFEKVPKGDAIFMKWILHDWSDEHCVKLLKNCYSAIPDDGKVIVMEGVVPTV 309

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNS-KPLLTVAGFKCIKFECFVCNLWIM 120
            ETT+A     K+++Q D+LMMTQNPGGKER  +  K L T AGFK IK  CF+CN WI+
Sbjct: 310 PETTAA----EKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKLVCFLCNFWII 369

Query: 121 EFFK 122
           EF K
Sbjct: 370 EFLK 369

[Close]

BLAST of MC04g0681 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 122.5 bits (306), Expect = 2.4e-28
Identity = 64/124 (51.61%), Postives = 82/124 (66.13%), Query Frame = 0


Query: 1   GVEHIGGDMFQS-PKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           G+EH+GGDMF S PKGD+IFMKWI HDWSDEH VK LKNC+ ++P+DGK+I+ E +LP  
Sbjct: 242 GIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLPEDGKVILAECILP-- 301

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKER-EMNSKPLLTVAGFKCIKFECFVCNLWIM 120
            ET  +  S  K V   D +M+  NPGGKER E   + L   +GFK IK  C    + ++
Sbjct: 302 -ETPDSSLS-TKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLI 361

Query: 121 EFFK 123
           E  K
Sbjct: 362 ELLK 361

[Close]

BLAST of MC04g0681 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 104.4 bits (259), Expect = 6.7e-23
Identity = 54/124 (43.55%), Postives = 79/124 (63.71%), Query Frame = 0


Query: 1   GVEHIGGDMF-QSPKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GV+H+ GDMF + PKGD+IFMKWILHDW DE  +K+LKNC  ++P+ GK+I+VE++ PM 
Sbjct: 261 GVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEKGKVIIVEMITPME 320

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNS-KPLLTVAGFKCIKFECFVCNLWIM 120
            +          +V   D+LM+TQ  GGKER ++  + L   +GF   +  C   +  ++
Sbjct: 321 PKPND---FSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYSVI 380

Query: 121 EFFK 123
           EF K
Sbjct: 381 EFHK 381

[Close]

BLAST of MC04g0681 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 104.0 bits (258), Expect = 8.8e-23
Identity = 55/124 (44.35%), Postives = 78/124 (62.90%), Query Frame = 0


Query: 1   GVEHIGGDMF-QSPKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEH+ GDMF + PKGD++FMKWILHDW DE  +K+LKNC  ++P+ GKII+VE V P  
Sbjct: 261 GVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLPEKGKIIIVEFVTP-- 320

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKEREMNS-KPLLTVAGFKCIKFECFVCNLWIM 120
            +        + +V   D+LM+TQ  GGKER ++  + L   +GF   +  C   +  ++
Sbjct: 321 -KEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVI 380

Query: 121 EFFK 123
           EF K
Sbjct: 381 EFHK 381

[Close]

BLAST of MC04g0681 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 103.6 bits (257), Expect = 1.1e-22
Identity = 56/123 (45.53%), Postives = 77/123 (62.60%), Query Frame = 0


Query: 1   GVEHIGGDMF-QSPKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEHIGGDMF + P+G+ I MKWILHDW+DE  V++LKNC  A+P+ G+IIV+E+++P  
Sbjct: 242 GVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALPETGRIIVIEMIVPRE 301

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKER-EMNSKPLLTVAGFKCIKFECFVCNLWIM 120
           V  T       K+    D+ MM+   GGKER +   + L   AGFK  K      + WI+
Sbjct: 302 VSETDL---ATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKIIYGAYSYWII 361

Query: 121 EFF 122
           E +
Sbjct: 362 ELY 361

[Close]

BLAST of MC04g0681 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 103.6 bits (257), Expect = 1.1e-22
Identity = 56/123 (45.53%), Postives = 77/123 (62.60%), Query Frame = 0


Query: 1   GVEHIGGDMF-QSPKGDSIFMKWILHDWSDEHRVKLLKNCHAAIPDDGKIIVVEVVLPMV 60
           GVEHIGGDMF + P+G+ I MKWILHDW+DE  V++LKNC  A+P+ G+IIV+E+++P  
Sbjct: 242 GVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALPETGRIIVIEMIVPRE 301

Query: 61  VETTSAVKSVAKSVAQDDVLMMTQNPGGKER-EMNSKPLLTVAGFKCIKFECFVCNLWIM 120
           V  T       K+    D+ MM+   GGKER +   + L   AGFK  K      + WI+
Sbjct: 302 VSETDL---ATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKIIYGAYSYWII 361

Query: 121 EFF 122
           E +
Sbjct: 362 ELY 361

The following BLAST results are available for this feature:

BLAST of MC04g0681 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGW0	7.2e-30	55.65	Caffeic acid 3-O-methyltransferase 1 OS=Ocimum basilicum OX=39350 GN=COMT1 PE=2 ...	[more]
O23760	9.4e-30	54.03	Caffeic acid 3-O-methyltransferase OS=Clarkia breweri OX=36903 GN=COMT PE=1 SV=1	[more]
Q8GU25	1.6e-29	52.42	Caffeic acid 3-O-methyltransferase OS=Rosa chinensis OX=74649 GN=COMT1 PE=2 SV=1	[more]
Q8LL87	2.1e-29	54.84	Caffeic acid 3-O-methyltransferase OS=Coffea canephora OX=49390 GN=COMT PE=2 SV=...	[more]
P28002	2.7e-29	52.42	Caffeic acid 3-O-methyltransferase OS=Medicago sativa OX=3879 PE=1 SV=1	[more]

BLAST of MC04g0681 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023540486.1	4.07e-50	69.35	caffeic acid 3-O-methyltransferase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022143497.1	2.47e-49	70.16	caffeic acid 3-O-methyltransferase-like [Momordica charantia]	[more]
XP_022143528.1	2.78e-49	69.35	caffeic acid 3-O-methyltransferase-like [Momordica charantia]	[more]
XP_011468886.1	7.46e-49	69.35	PREDICTED: LOW QUALITY PROTEIN: caffeic acid 3-O-methyltransferase-like [Fragari...	[more]
PNX97772.1	9.02e-49	66.13	caffeic acid 3-O-methyltransferase-like protein [Trifolium pratense]	[more]

BLAST of MC04g0681 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQX8	1.35e-49	69.35	caffeic acid 3-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1CQZ7	3.36e-49	70.16	caffeic acid 3-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A2K3N417	4.37e-49	66.13	Caffeic acid 3-O-methyltransferase-like protein OS=Trifolium pratense OX=57577 G...	[more]
A0A2P6SIY5	1.26e-48	68.55	Putative caffeate O-methyltransferase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr...	[more]
A0A6J1FBC5	1.73e-48	67.74	caffeic acid 3-O-methyltransferase-like isoform X3 OS=Cucurbita moschata OX=3662...	[more]

BLAST of MC04g0681 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54160.1	2.4e-28	51.61	O-methyltransferase 1	[more]
AT1G77530.1	6.7e-23	43.55	O-methyltransferase family protein	[more]
AT1G77520.1	8.8e-23	44.35	O-methyltransferase family protein	[more]
AT1G51990.1	1.1e-22	45.53	O-methyltransferase family protein	[more]
AT1G51990.2	1.1e-22	45.53	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..111 e-value: 1.4E-37 score: 131.8
None	No IPR available	PANTHER	PTHR11746:SF169	CAFFEIC ACID O-METHYLTRANSFERASE	coord: 1..122
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 2..104 e-value: 5.2E-25 score: 88.0
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 1..122
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 1..122 score: 24.054136
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 1..120

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0681.1	MC04g0681.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0030755	quercetin 3-O-methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity