MC04g0649 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0649
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	ERAD-associated E3 ubiquitin-protein ligase HRD1-like
Location	MC04: 5979846 .. 5980538 (-)
RNA-Seq Expression	MC04g0649
Synteny	MC04g0649

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATATCAAATCTCCCGCTATTCAAATCTCATAGTCGAACTGCAGATTCCTTCACCGGAAACCGAACAGCCTCCGCAAAATCCATTGCCGATCGAACAAATCGGCCTCGTCTTCACCTTCCGGAACAAACGCAGCTGCTTCGACGTAGCAGAGGACGGCGAATGGACGCTGGTATGGCAATGGCAAGACCTACGAATTCCACTCTGCGACCACCAATTCCCGGCCGCAATCTTCAATCGCGGCGGCGACTCCCTCTTCCGCCGCCTCGCCTGGAGTTTCGATCGATTCGAGCAAGAAATCGACGCCGAAATCGTCGCCGACGCCCTAATCGAGCGGTGGATTCAGATCAGAGACGAAGCGAATTCGAGAAGCTCGAGCAGAGTATTCGCGATGACAGTGGATTTGATTCTGTGGAGCGTCGAGGCGCGCCATGAAATTAGACCGGATGGAATGAGCGTGGCGGCGGGAGAAACCGGAATGGTGCCGGCGTGCGGCGGAGCGGTGACGGAGATGTTGAGGAGAATCGAAATCGAAGAACAGGCGAGAAATTGTGTGATCTGTTTGGAGGGATTGAGAAAGAAGGGGGAATTTGGTTTGCAAATGCCGTGTTTACATGTGTTTCATGGACGGTGCATACGAGAATGGCTGAAGAACAGCCATTACTGTCCAATTTGCAGGTTTCAAATGCCT

mRNA sequence

ATGGAATATCAAATCTCCCGCTATTCAAATCTCATAGTCGAACTGCAGATTCCTTCACCGGAAACCGAACAGCCTCCGCAAAATCCATTGCCGATCGAACAAATCGGCCTCGTCTTCACCTTCCGGAACAAACGCAGCTGCTTCGACGTAGCAGAGGACGGCGAATGGACGCTGGTATGGCAATGGCAAGACCTACGAATTCCACTCTGCGACCACCAATTCCCGGCCGCAATCTTCAATCGCGGCGGCGACTCCCTCTTCCGCCGCCTCGCCTGGAGTTTCGATCGATTCGAGCAAGAAATCGACGCCGAAATCGTCGCCGACGCCCTAATCGAGCGGTGGATTCAGATCAGAGACGAAGCGAATTCGAGAAGCTCGAGCAGAGTATTCGCGATGACAGTGGATTTGATTCTGTGGAGCGTCGAGGCGCGCCATGAAATTAGACCGGATGGAATGAGCGTGGCGGCGGGAGAAACCGGAATGGTGCCGGCGTGCGGCGGAGCGGTGACGGAGATGTTGAGGAGAATCGAAATCGAAGAACAGGCGAGAAATTGTGTGATCTGTTTGGAGGGATTGAGAAAGAAGGGGGAATTTGGTTTGCAAATGCCGTGTTTACATGTGTTTCATGGACGGTGCATACGAGAATGGCTGAAGAACAGCCATTACTGTCCAATTTGCAGGTTTCAAATGCCT

Coding sequence (CDS)

ATGGAATATCAAATCTCCCGCTATTCAAATCTCATAGTCGAACTGCAGATTCCTTCACCGGAAACCGAACAGCCTCCGCAAAATCCATTGCCGATCGAACAAATCGGCCTCGTCTTCACCTTCCGGAACAAACGCAGCTGCTTCGACGTAGCAGAGGACGGCGAATGGACGCTGGTATGGCAATGGCAAGACCTACGAATTCCACTCTGCGACCACCAATTCCCGGCCGCAATCTTCAATCGCGGCGGCGACTCCCTCTTCCGCCGCCTCGCCTGGAGTTTCGATCGATTCGAGCAAGAAATCGACGCCGAAATCGTCGCCGACGCCCTAATCGAGCGGTGGATTCAGATCAGAGACGAAGCGAATTCGAGAAGCTCGAGCAGAGTATTCGCGATGACAGTGGATTTGATTCTGTGGAGCGTCGAGGCGCGCCATGAAATTAGACCGGATGGAATGAGCGTGGCGGCGGGAGAAACCGGAATGGTGCCGGCGTGCGGCGGAGCGGTGACGGAGATGTTGAGGAGAATCGAAATCGAAGAACAGGCGAGAAATTGTGTGATCTGTTTGGAGGGATTGAGAAAGAAGGGGGAATTTGGTTTGCAAATGCCGTGTTTACATGTGTTTCATGGACGGTGCATACGAGAATGGCTGAAGAACAGCCATTACTGTCCAATTTGCAGGTTTCAAATGCCT

Protein sequence

MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVWQWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDEANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEIEEQARNCVICLEGLRKKGEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP

Homology

[Close]

BLAST of MC04g0649 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 36/95 (37.89%), Postives = 52/95 (54.74%), Query Frame = 0


Query: 141 VEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEIEE----QARNCVICLEGLRKKG 200
           +E   +I   G  +  G +G  PA   A+ E L R+EI +       NC +C E + + G
Sbjct: 170 LEQLSQIEASGNGI--GRSGNPPASKSAI-ESLPRVEISDCHTKAEANCAVCTE-VFEAG 229

Query: 201 EFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 232
             G +MPC H+FHG CI  WL   + CP+CRF++P
Sbjct: 230 IEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of MC04g0649 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.0e-09
Identity = 31/86 (36.05%), Postives = 45/86 (52.33%), Query Frame = 0


Query: 150 DGMSVAAGETGMVPACGGAVTEMLRRIEIEE----QARNCVICLEGLRKKGEFGLQMPCL 209
           +  +   G +G  PA   A+ E L R+EI +       NC +C E    + E   +MPC 
Sbjct: 161 EASATGIGRSGNPPASKSAI-ESLPRVEISDCHIGSEANCAVCTEIFETETE-AREMPCK 220

Query: 210 HVFHGRCIREWLKNSHYCPICRFQMP 232
           H+FH  CI  WL   + CP+CRF++P
Sbjct: 221 HLFHDDCIVPWLSIRNSCPVCRFELP 244

[Close]

BLAST of MC04g0649 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.7e-08
Identity = 30/78 (38.46%), Postives = 41/78 (52.56%), Query Frame = 0


Query: 157 GETGMVPACGGAVTEMLRRIEIE----EQARNCVICLEGLRKKGEFGLQMPCLHVFHGRC 216
           G  G  PA   A+ E L   E+     E    C +C +G+   GE G ++PC H +HG C
Sbjct: 226 GRRGAPPAAKSAI-EALETFEVSSSEGEMVMVCAVCKDGM-VMGETGKKLPCGHCYHGDC 285

Query: 217 IREWLKNSHYCPICRFQM 231
           I  WL   + CP+CRFQ+
Sbjct: 286 IVPWLGTRNSCPVCRFQL 301

[Close]

BLAST of MC04g0649 vs. ExPASy Swiss-Prot
Match: Q8GXF8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SGR9 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.7e-08
Identity = 32/76 (42.11%), Postives = 41/76 (53.95%), Query Frame = 0


Query: 162 VPACGGAVTEMLRRIEIEEQARN------CVICLEGLRKKGEFGLQMPCLHVFHGRCIRE 221
           VPA   AV   LR +E+   A +      CVIC E +  +G    +MPC H FH +CI  
Sbjct: 186 VPAARAAVV-ALRAVEVFNVAASNAGEVECVICKEEM-SEGRDVCEMPCQHFFHWKCILP 245

Query: 222 WLKNSHYCPICRFQMP 232
           WL   + CP CRFQ+P
Sbjct: 246 WLSKKNTCPFCRFQLP 259

[Close]

BLAST of MC04g0649 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.3e-07
Identity = 25/70 (35.71%), Postives = 39/70 (55.71%), Query Frame = 0


Query: 163 PACGGAVTEMLRRIEIEEQAR-NCVICLEGLRKKGEFGLQMPCLHVFHGRCIREWLKNSH 222
           PA    V  + R +    QA   C +CL    ++ E  ++MPC H+FH  CI  WL  ++
Sbjct: 53  PAAKTVVENLPRTVIRGSQAELKCPVCLLEFEEE-ETAIEMPCHHLFHSSCILPWLSKTN 112

Query: 223 YCPICRFQMP 232
            CP+CR+++P
Sbjct: 113 SCPLCRYELP 121

[Close]

BLAST of MC04g0649 vs. NCBI nr
Match: XP_022972455.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita maxima])

HSP 1 Score: 287 bits (735), Expect = 7.77e-95
Identity = 144/236 (61.02%), Postives = 176/236 (74.58%), Query Frame = 0


Query: 1   MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVW 60
           ME+QISRY NL+VE+    P   + P++P  IE IGL+FT R++RS F+V+E GE  L+ 
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRHRRSHFEVSESGERRLIQ 60

Query: 61  QWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDE 120
           Q + L+IPLCD+QF A IFNR  DSLF  L+ SF  F+ EIDAE VADALIERWI+IRDE
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHNLSSSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 ANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEIEE 180
           ANSRSS RVF MTV+L +W + AR+EI   G+       GMVPACGGAV +MLRR+E E+
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEI---GVGSGGDGCGMVPACGGAVGKMLRRLERED 180

Query: 181 QARN-CVICLEGLRKK----GEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 231
           +    CVICL+ L+KK    G FGLQMPCLHVFHG CI +WLKNSHYCPICRF++P
Sbjct: 181 EVDGICVICLDELKKKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVP 233

[Close]

BLAST of MC04g0649 vs. NCBI nr
Match: KAG6571896.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 285 bits (730), Expect = 3.65e-94
Identity = 143/236 (60.59%), Postives = 175/236 (74.15%), Query Frame = 0


Query: 1   MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVW 60
           ME+QISRY NL+VE+    P   + P++P  IE IGL+FT R +RS F+V+E+GE  L+ 
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  QWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDE 120
           Q + L+IPLCD+QF A IFNR  DSLF  L+ SF  F+ EIDAE VADALIERWI+IRDE
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 ANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEIEE 180
           ANSRSS RVF MTV+L +W + AR+EI   G+       GMVPACGGAV +MLRR+E E+
Sbjct: 121 ANSRSSDRVFPMTVNLTIWHIVARYEI---GVGSGGDGCGMVPACGGAVGKMLRRLERED 180

Query: 181 QARN-CVICLEGLRKK----GEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 231
           +    CVICL+ L+ K    G FGLQMPCLHVFHG CI +WLKNSHYCPICRF++P
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVP 233

[Close]

BLAST of MC04g0649 vs. NCBI nr
Match: XP_022952293.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata] >KAG7011584.1 HRD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 285 bits (728), Expect = 7.35e-94
Identity = 142/236 (60.17%), Postives = 175/236 (74.15%), Query Frame = 0


Query: 1   MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVW 60
           ME+QISRY NL+VE+    P   + P++P  IE IGL+FT R +RS F+V+E+GE  L+ 
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  QWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDE 120
           Q + L+IPLCD+QF A IFNR  DSLF  L+ SF  F+ EIDAE VADALIERWI+IRDE
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 ANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEIEE 180
           ANSRSS RVF MTV+L +W + AR+EI   G+       GMVPACGGAV +MLRR++ E+
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEI---GVGSGGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 181 QARN-CVICLEGLRKK----GEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 231
           +    CVICL+ L+ K    G FGLQMPCLHVFHG CI +WLKNSHYCPICRF++P
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVP 233

[Close]

BLAST of MC04g0649 vs. NCBI nr
Match: XP_023511497.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 280 bits (715), Expect = 6.95e-92
Identity = 140/236 (59.32%), Postives = 174/236 (73.73%), Query Frame = 0


Query: 1   MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVW 60
           ME+QISRY +L+VE+    P   + P++P  IE IGL+FT R +RS F+V+E+GE  L+ 
Sbjct: 1   MEHQISRYESLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  QWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDE 120
           Q + L+IPLCD+QF A IFNR  DSLF  L+ SF  F+ EIDAE VADALIERWI+IRDE
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 ANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEIEE 180
           ANSRSS RVF MTV+L +W + AR+EI   G+       GMVPACGGAV +MLRR++ E+
Sbjct: 121 ANSRSSDRVFPMTVNLTIWHIVARYEI---GVGSGGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 181 QARN-CVICLEGLRKK----GEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 231
           +    CVICL+ L+ K    G F LQMPCLHVFHG CI +WLKNSHYCPICRF++P
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFTLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVP 233

[Close]

BLAST of MC04g0649 vs. NCBI nr
Match: XP_016901804.1 (PREDICTED: RING finger protein 141-like [Cucumis melo] >KAA0025781.1 RING finger protein 141-like [Cucumis melo var. makuwa] >TYK09674.1 RING finger protein 141-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 249 bits (636), Expect = 8.99e-80
Identity = 124/234 (52.99%), Postives = 164/234 (70.09%), Query Frame = 0


Query: 1   MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVW 60
           MEYQIS+YS   VE+ I  P + +  ++P PIE IGL+FT R + + F V E+GE  LV 
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  QWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDE 120
           + +  ++PLCDHQFP  IFN   +SLF+ L  SF+ +E +IDAE+VA ALIE+WI IRD+
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 ANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEI-E 180
            NSRS  +VF+MTV+L +  V  ++ I  DG S    +   VPAC  A+ +ML+R+E+ +
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EQARNCVICLEGLRKK--GEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 231
           E  R C+ICL+ +RKK  G  GL+MPCLHVFHG+CI  WLKNSH CPICRFQMP
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMP 234

[Close]

BLAST of MC04g0649 vs. ExPASy TrEMBL
Match: A0A6J1I8N9 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita maxima OX=3661 GN=LOC111471014 PE=4 SV=1)

HSP 1 Score: 287 bits (735), Expect = 3.76e-95
Identity = 144/236 (61.02%), Postives = 176/236 (74.58%), Query Frame = 0


Query: 1   MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVW 60
           ME+QISRY NL+VE+    P   + P++P  IE IGL+FT R++RS F+V+E GE  L+ 
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRHRRSHFEVSESGERRLIQ 60

Query: 61  QWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDE 120
           Q + L+IPLCD+QF A IFNR  DSLF  L+ SF  F+ EIDAE VADALIERWI+IRDE
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHNLSSSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 ANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEIEE 180
           ANSRSS RVF MTV+L +W + AR+EI   G+       GMVPACGGAV +MLRR+E E+
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEI---GVGSGGDGCGMVPACGGAVGKMLRRLERED 180

Query: 181 QARN-CVICLEGLRKK----GEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 231
           +    CVICL+ L+KK    G FGLQMPCLHVFHG CI +WLKNSHYCPICRF++P
Sbjct: 181 EVDGICVICLDELKKKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVP 233

[Close]

BLAST of MC04g0649 vs. ExPASy TrEMBL
Match: A0A6J1GK53 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita moschata OX=3662 GN=LOC111455016 PE=4 SV=1)

HSP 1 Score: 285 bits (728), Expect = 3.56e-94
Identity = 142/236 (60.17%), Postives = 175/236 (74.15%), Query Frame = 0


Query: 1   MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVW 60
           ME+QISRY NL+VE+    P   + P++P  IE IGL+FT R +RS F+V+E+GE  L+ 
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  QWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDE 120
           Q + L+IPLCD+QF A IFNR  DSLF  L+ SF  F+ EIDAE VADALIERWI+IRDE
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 ANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEIEE 180
           ANSRSS RVF MTV+L +W + AR+EI   G+       GMVPACGGAV +MLRR++ E+
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEI---GVGSGGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 181 QARN-CVICLEGLRKK----GEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 231
           +    CVICL+ L+ K    G FGLQMPCLHVFHG CI +WLKNSHYCPICRF++P
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVP 233

[Close]

BLAST of MC04g0649 vs. ExPASy TrEMBL
Match: A0A5A7SNF1 (RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00770 PE=4 SV=1)

HSP 1 Score: 249 bits (636), Expect = 4.35e-80
Identity = 124/234 (52.99%), Postives = 164/234 (70.09%), Query Frame = 0


Query: 1   MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVW 60
           MEYQIS+YS   VE+ I  P + +  ++P PIE IGL+FT R + + F V E+GE  LV 
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  QWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDE 120
           + +  ++PLCDHQFP  IFN   +SLF+ L  SF+ +E +IDAE+VA ALIE+WI IRD+
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 ANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEI-E 180
            NSRS  +VF+MTV+L +  V  ++ I  DG S    +   VPAC  A+ +ML+R+E+ +
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EQARNCVICLEGLRKK--GEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 231
           E  R C+ICL+ +RKK  G  GL+MPCLHVFHG+CI  WLKNSH CPICRFQMP
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMP 234

[Close]

BLAST of MC04g0649 vs. ExPASy TrEMBL
Match: A0A0A0L236 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 PE=4 SV=1)

HSP 1 Score: 249 bits (636), Expect = 4.35e-80
Identity = 121/234 (51.71%), Postives = 163/234 (69.66%), Query Frame = 0


Query: 1   MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVW 60
           MEYQISRYS   VE+ I  P+  + P++P PIE IGL+FT R + + F V E+GE  LV 
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  QWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDE 120
           + +  ++PLCDHQFP  IFN    SLF+ L  SF+ +E +IDAE+VA  LIE+WI+IR++
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 121 ANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEI-E 180
            NSRS  +VF+MTV+L +  V  ++ +  DG +    +  +VPAC  A+ +ML+R+E+ E
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 181 EQARNCVICLE--GLRKKGEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 231
           E  R CVICL+  G +K G  GL+MPCLHVFHG+CI  W KNSH CPICRF+MP
Sbjct: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMP 234

[Close]

BLAST of MC04g0649 vs. ExPASy TrEMBL
Match: A0A1S4E0Q9 (RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1)

HSP 1 Score: 249 bits (636), Expect = 4.35e-80
Identity = 124/234 (52.99%), Postives = 164/234 (70.09%), Query Frame = 0


Query: 1   MEYQISRYSNLIVELQIPSPETEQPPQNPLPIEQIGLVFTFRNKRSCFDVAEDGEWTLVW 60
           MEYQIS+YS   VE+ I  P + +  ++P PIE IGL+FT R + + F V E+GE  LV 
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  QWQDLRIPLCDHQFPAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDE 120
           + +  ++PLCDHQFP  IFN   +SLF+ L  SF+ +E +IDAE+VA ALIE+WI IRD+
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 ANSRSSSRVFAMTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEI-E 180
            NSRS  +VF+MTV+L +  V  ++ I  DG S    +   VPAC  A+ +ML+R+E+ +
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EQARNCVICLEGLRKK--GEFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 231
           E  R C+ICL+ +RKK  G  GL+MPCLHVFHG+CI  WLKNSH CPICRFQMP
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMP 234

[Close]

BLAST of MC04g0649 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.0e-11
Identity = 36/95 (37.89%), Postives = 52/95 (54.74%), Query Frame = 0


Query: 141 VEARHEIRPDGMSVAAGETGMVPACGGAVTEMLRRIEIEE----QARNCVICLEGLRKKG 200
           +E   +I   G  +  G +G  PA   A+ E L R+EI +       NC +C E + + G
Sbjct: 170 LEQLSQIEASGNGI--GRSGNPPASKSAI-ESLPRVEISDCHTKAEANCAVCTE-VFEAG 229

Query: 201 EFGLQMPCLHVFHGRCIREWLKNSHYCPICRFQMP 232
             G +MPC H+FHG CI  WL   + CP+CRF++P
Sbjct: 230 IEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of MC04g0649 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 5.0e-11
Identity = 30/78 (38.46%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 157 GETGMVPACGGAVTEMLRRI---EIEEQARNCVICLEGLRKKGEFGLQMPCLHVFHGRCI 216
           G+ G  PA    V  M R +   + E+   +C ICL+    KG+   +MPC H FH +C+
Sbjct: 78  GKQGRSPALKSEVENMPRVVIGEDKEKYGGSCAICLDEW-SKGDVAAEMPCKHKFHSKCV 137

Query: 217 REWLKNSHYCPICRFQMP 232
            EWL     CP+CR++MP
Sbjct: 138 EEWLGRHATCPMCRYEMP 154

[Close]

BLAST of MC04g0649 vs. TAIR 10
Match: AT2G37150.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 8.6e-11
Identity = 48/161 (29.81%), Postives = 71/161 (44.10%), Query Frame = 0


Query: 75  PAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDEANSRSSSRVFA--- 134
           P  I  R GD   RR + S++RF+   D   + +        + D       SR      
Sbjct: 379 PPHISWRSGDRPGRRRS-SYERFQPPFDEVALHERFSSEEFMVEDRQPQYYGSRNMLDHH 438

Query: 135 --MTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGA---VTEMLRRIEIEEQARNCV 194
             M +D+   S E   E+   G  + +  TG+  +   +    T      + EEQ R C 
Sbjct: 439 RDMRLDIDNMSYE---ELLDLGERIGSVNTGLSDSAISSCLLATMYYPSYQTEEQ-RKCA 498

Query: 195 ICLEGLRKKGEFGLQMPCLHVFHGRCIREWLKNSHYCPICR 228
           ICLE  ++K E G    C H +HGRCI++WL   + CPIC+
Sbjct: 499 ICLEEYKEKEELGEVKGCGHDYHGRCIKKWLSMKNSCPICK 534

[Close]

BLAST of MC04g0649 vs. TAIR 10
Match: AT2G37150.2 (RING/U-box superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 8.6e-11
Identity = 48/161 (29.81%), Postives = 71/161 (44.10%), Query Frame = 0


Query: 75  PAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDEANSRSSSRVFA--- 134
           P  I  R GD   RR + S++RF+   D   + +        + D       SR      
Sbjct: 379 PPHISWRSGDRPGRRRS-SYERFQPPFDEVALHERFSSEEFMVEDRQPQYYGSRNMLDHH 438

Query: 135 --MTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGA---VTEMLRRIEIEEQARNCV 194
             M +D+   S E   E+   G  + +  TG+  +   +    T      + EEQ R C 
Sbjct: 439 RDMRLDIDNMSYE---ELLDLGERIGSVNTGLSDSAISSCLLATMYYPSYQTEEQ-RKCA 498

Query: 195 ICLEGLRKKGEFGLQMPCLHVFHGRCIREWLKNSHYCPICR 228
           ICLE  ++K E G    C H +HGRCI++WL   + CPIC+
Sbjct: 499 ICLEEYKEKEELGEVKGCGHDYHGRCIKKWLSMKNSCPICK 534

[Close]

BLAST of MC04g0649 vs. TAIR 10
Match: AT2G37150.3 (RING/U-box superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 8.6e-11
Identity = 48/161 (29.81%), Postives = 71/161 (44.10%), Query Frame = 0


Query: 75  PAAIFNRGGDSLFRRLAWSFDRFEQEIDAEIVADALIERWIQIRDEANSRSSSRVFA--- 134
           P  I  R GD   RR + S++RF+   D   + +        + D       SR      
Sbjct: 432 PPHISWRSGDRPGRRRS-SYERFQPPFDEVALHERFSSEEFMVEDRQPQYYGSRNMLDHH 491

Query: 135 --MTVDLILWSVEARHEIRPDGMSVAAGETGMVPACGGA---VTEMLRRIEIEEQARNCV 194
             M +D+   S E   E+   G  + +  TG+  +   +    T      + EEQ R C 
Sbjct: 492 RDMRLDIDNMSYE---ELLDLGERIGSVNTGLSDSAISSCLLATMYYPSYQTEEQ-RKCA 551

Query: 195 ICLEGLRKKGEFGLQMPCLHVFHGRCIREWLKNSHYCPICR 228
           ICLE  ++K E G    C H +HGRCI++WL   + CPIC+
Sbjct: 552 ICLEEYKEKEELGEVKGCGHDYHGRCIKKWLSMKNSCPICK 587

The following BLAST results are available for this feature:

BLAST of MC04g0649 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SNB6	1.4e-10	37.89	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q940T5	6.0e-09	36.05	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q9SPL2	1.7e-08	38.46	E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV...	[more]
Q8GXF8	1.7e-08	42.11	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9P0P0	4.3e-07	35.71	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1	[more]

BLAST of MC04g0649 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022972455.1	7.77e-95	61.02	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita maxima]	[more]
KAG6571896.1	3.65e-94	60.59	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022952293.1	7.35e-94	60.17	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata] >KAG7...	[more]
XP_023511497.1	6.95e-92	59.32	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pep...	[more]
XP_016901804.1	8.99e-80	52.99	PREDICTED: RING finger protein 141-like [Cucumis melo] >KAA0025781.1 RING finger...	[more]

BLAST of MC04g0649 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I8N9	3.76e-95	61.02	ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita maxima OX=366...	[more]
A0A6J1GK53	3.56e-94	60.17	ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita moschata OX=3...	[more]
A0A5A7SNF1	4.35e-80	52.99	RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A0A0L236	4.35e-80	51.71	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 P...	[more]
A0A1S4E0Q9	4.35e-80	52.99	RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1	[more]

BLAST of MC04g0649 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46620.1	1.0e-11	37.89	zinc finger (C3HC4-type RING finger) family protein	[more]
AT1G14200.1	5.0e-11	38.46	RING/U-box superfamily protein	[more]
AT2G37150.1	8.6e-11	29.81	RING/U-box superfamily protein	[more]
AT2G37150.2	8.6e-11	29.81	RING/U-box superfamily protein	[more]
AT2G37150.3	8.6e-11	29.81	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 185..226 e-value: 4.9E-7 score: 39.4
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 184..227 e-value: 3.4E-11 score: 43.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 185..227 score: 12.533537
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 168..231 e-value: 5.5E-16 score: 59.6
None	No IPR available	PANTHER	PTHR15710:SF63	RING/U-BOX SUPERFAMILY PROTEIN	coord: 112..231
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 112..231
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 179..230

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0649.1	MC04g0649.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity