MC04g0533 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0533
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	calmodulin-like protein 1
Location	MC04: 4570938 .. 4571471 (-)
RNA-Seq Expression	MC04g0533
Synteny	MC04g0533

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGAACCTGAATCTTATGAACCTGCAGTACAACGTGTCGAAGAAAAAGTTCCTCCGAGAACCGTCGCGGCTGTTCTCGTTCAGAGACCGGCAGAGCTCCGGCCTCCGGCGGGCGCTGCAGCCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGAGAATCTCGAAGCAGGAGTACAGGGGAATTCTGAAGGCCCTGGGGAGGGGGAACTCAATGGAAGAGGTGCAGAAGATCTTCAGGGCGGTGGACGGCGACGGCGATGGGTTCATAAATTTGAACGAGTTCATGGAAGTTCACCGGAGTGGCGGCGGAGTTCAGGCGAGGGAGGTGGAGTATGCGTTCCGAACGTTCGATTTGAACGGCGATCGGAAGATAAGTGCGGAGGAGGTGATGAGGGTATTGAGAGGATTGGGGGAAAACTGTAGCCTCGAGGACTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGCATGGTGGACATTGATGAGTTCATGACTATGATGACT

mRNA sequence

ACGAACCTGAATCTTATGAACCTGCAGTACAACGTGTCGAAGAAAAAGTTCCTCCGAGAACCGTCGCGGCTGTTCTCGTTCAGAGACCGGCAGAGCTCCGGCCTCCGGCGGGCGCTGCAGCCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGAGAATCTCGAAGCAGGAGTACAGGGGAATTCTGAAGGCCCTGGGGAGGGGGAACTCAATGGAAGAGGTGCAGAAGATCTTCAGGGCGGTGGACGGCGACGGCGATGGGTTCATAAATTTGAACGAGTTCATGGAAGTTCACCGGAGTGGCGGCGGAGTTCAGGCGAGGGAGGTGGAGTATGCGTTCCGAACGTTCGATTTGAACGGCGATCGGAAGATAAGTGCGGAGGAGGTGATGAGGGTATTGAGAGGATTGGGGGAAAACTGTAGCCTCGAGGACTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGCATGGTGGACATTGATGAGTTCATGACTATGATGACT

Coding sequence (CDS)

ACGAACCTGAATCTTATGAACCTGCAGTACAACGTGTCGAAGAAAAAGTTCCTCCGAGAACCGTCGCGGCTGTTCTCGTTCAGAGACCGGCAGAGCTCCGGCCTCCGGCGGGCGCTGCAGCCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGAGAATCTCGAAGCAGGAGTACAGGGGAATTCTGAAGGCCCTGGGGAGGGGGAACTCAATGGAAGAGGTGCAGAAGATCTTCAGGGCGGTGGACGGCGACGGCGATGGGTTCATAAATTTGAACGAGTTCATGGAAGTTCACCGGAGTGGCGGCGGAGTTCAGGCGAGGGAGGTGGAGTATGCGTTCCGAACGTTCGATTTGAACGGCGATCGGAAGATAAGTGCGGAGGAGGTGATGAGGGTATTGAGAGGATTGGGGGAAAACTGTAGCCTCGAGGACTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGCATGGTGGACATTGATGAGTTCATGACTATGATGACT

Protein sequence

TNLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGRISKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYAFRTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT

Homology

[Close]

BLAST of MC04g0533 vs. ExPASy Swiss-Prot
Match: Q9ZQE6 (Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 8.3e-51
Identity = 100/183 (54.64%), Postives = 143/183 (78.14%), Query Frame = 0


Query: 1   TNLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRA-----LQPSTEEIKQIFNKFDT 60
           +N++ + LQY +SK K LR+PSR+FS RDRQSSGL         QPS  E++++F++FD 
Sbjct: 2   SNVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDL 61

Query: 61  NRDGRISKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAR 120
           ++DG+IS+ EY+ +L+ALG+  ++E+V KIF+AVD DGDGFI+  EF++ ++  GG+++ 
Sbjct: 62  DKDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSS 121

Query: 121 EVEYAFRTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMT 179
           ++  +F TFDLNGD KISAEEVM VL  LGE CSLEDC RMVRAVD+DGDG+V+++EF+ 
Sbjct: 122 DIRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIK 181

[Close]

BLAST of MC04g0533 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 4.9e-27
Identity = 63/152 (41.45%), Postives = 95/152 (62.50%), Query Frame = 0


Query: 29  DRQSSGLRRALQPSTEEIKQIFNKFDTNRDGRISKQEYRGILKALGRGNSMEEVQKIFRA 88
           D  S G   A  P  EE++++F KFD N DGRIS+ E   + ++LG   + +E+ ++   
Sbjct: 25  DPASGGGSPAPTPE-EEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAE 84

Query: 89  VDGDGDGFINLNEFMEVHRSGGGVQA---REVEYAFRTFDLNGDRKISAEEVMRVLRGLG 148
            D DGDGFI+L+EF  ++ +  G  A    ++ +AFR FD +G+  ISA E+ RVL GLG
Sbjct: 85  ADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLG 144

Query: 149 ENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMM 178
           E  +++ CRRM+  VD +GDG++  +EF  MM
Sbjct: 145 EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]

BLAST of MC04g0533 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.4e-26
Identity = 62/150 (41.33%), Postives = 95/150 (63.33%), Query Frame = 0


Query: 32  SSGLRRALQPSTEEIKQIFNKFDTNRDGRISKQEYRGILKALGRGNSMEEVQKIFRAVDG 91
           S  + R    S E+IK++F +FD N DG+IS  E + ++ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 92  DGDGFINLNEFMEV----HRSGGGVQAREVEYAFRTFDLNGDRKISAEEVMRVLRGLGEN 151
           DG+GFI+L+EF+ +     +S      R+++ AF  +DL+ + +ISA E+  V++ LGE 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 152 CSLEDCRRMVRAVDSDGDGMVDIDEFMTMM 178
           CS++DC+RM+  VDSDGDG VD +EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of MC04g0533 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.9e-26
Identity = 62/148 (41.89%), Postives = 92/148 (62.16%), Query Frame = 0


Query: 35  LRRALQPSTE--EIKQIFNKFDTNRDGRISKQEYRGILKALGRGNSMEEVQKIFRAVDGD 94
           L++A    TE  E++ +F KFD N DG+IS +E   I+ +LG     EE++K    +D  
Sbjct: 25  LQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRK 84

Query: 95  GDGFINLNEFMEVHRSGGGVQ--AREVEYAFRTFDLNGDRKISAEEVMRVLRGLGENCSL 154
           GDG+IN  EF+E++  G         ++ AF  +D++G+  ISAEE+  VLR LG+ CS+
Sbjct: 85  GDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSI 144

Query: 155 EDCRRMVRAVDSDGDGMVDIDEFMTMMT 179
            +CR+M+  VD DGDG +D +EF  MMT
Sbjct: 145 AECRKMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of MC04g0533 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 3.2e-26
Identity = 62/152 (40.79%), Postives = 100/152 (65.79%), Query Frame = 0


Query: 33  SGLRRALQPSTEEIKQIFNKFDTNRDGRISKQEYRGILKALGRGNSMEEVQKIFRAVDGD 92
           +G+ R+   S ++IK++F +FD N DG+IS  E + +++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 93  GDGFINLNEFM---EVHRSGGGVQAREV---EYAFRTFDLNGDRKISAEEVMRVLRGLGE 152
           G+GFI+L+EF+   ++   GGG    +V   + AF  +DL+G+ +ISA+E+  V++ LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 153 NCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 179
            CS++DC++M+  VD DGDG V+ DEF  MM+
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of MC04g0533 vs. NCBI nr
Match: XP_022136065.1 (calmodulin-like protein 1 [Momordica charantia])

HSP 1 Score: 348 bits (894), Expect = 8.09e-121
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   TNLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGR 60
           TNLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGR
Sbjct: 3   TNLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGR 62

Query: 61  ISKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYA 120
           ISKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYA
Sbjct: 63  ISKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYA 122

Query: 121 FRTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 178
           FRTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 123 FRTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

[Close]

BLAST of MC04g0533 vs. NCBI nr
Match: XP_022988967.1 (calmodulin-like protein 1 [Cucurbita maxima])

HSP 1 Score: 321 bits (822), Expect = 7.67e-110
Identity = 161/177 (90.96%), Postives = 171/177 (96.61%), Query Frame = 0


Query: 2   NLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGRI 61
           N NL++LQY VSKKKFLREPSRLFSF+DRQSSG+RRALQPSTEEIKQIFNKFDTNRDGRI
Sbjct: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGRI 63

Query: 62  SKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYAF 121
           SK EYRG+LKALGRGNSMEEVQKIFRAVD DGDG+INLNEFMEVHRSG GVQAREVE+AF
Sbjct: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQAREVEFAF 123

Query: 122 RTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 178
           +TFDLNGDRKISAEE+MRVLRGLGE CS+EDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

[Close]

BLAST of MC04g0533 vs. NCBI nr
Match: XP_004137114.2 (calmodulin-like protein 30 [Cucumis sativus] >KGN43936.1 hypothetical protein Csa_017094 [Cucumis sativus])

HSP 1 Score: 320 bits (819), Expect = 2.20e-109
Identity = 160/177 (90.40%), Postives = 171/177 (96.61%), Query Frame = 0


Query: 2   NLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGRI 61
           N N + LQYNVSKKKFLREPSRLFSF+DRQSSGLRRALQPS+EEIKQIFNKFDTN+DGRI
Sbjct: 4   NPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRI 63

Query: 62  SKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYAF 121
           SK EYRGILKALGRGNSMEEVQKIFRAVD DGDG+INLNEFMEVHRSGGGVQA+EVE+AF
Sbjct: 64  SKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAF 123

Query: 122 RTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 178
           +TFDLNGDRKISAEEVMRVL+GLGE CS+EDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 124 KTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

[Close]

BLAST of MC04g0533 vs. NCBI nr
Match: KAG6588656.1 (Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013282.1 Calmodulin-like protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 320 bits (819), Expect = 2.20e-109
Identity = 160/177 (90.40%), Postives = 171/177 (96.61%), Query Frame = 0


Query: 2   NLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGRI 61
           N NL++LQY VSKKKFLREPSRLFSF+DRQSSG+RRALQPSTEEIKQIFNKFDTNRDGRI
Sbjct: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGRI 63

Query: 62  SKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYAF 121
           SK EYRG+LKALGRGNSMEEVQKIFRAVD DGDG+INLNEFMEVHRSG GVQAR+VE+AF
Sbjct: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVEFAF 123

Query: 122 RTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 178
           +TFDLNGDRKISAEE+MRVLRGLGE CS+EDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

[Close]

BLAST of MC04g0533 vs. NCBI nr
Match: XP_038888181.1 (calmodulin-like protein 30 [Benincasa hispida])

HSP 1 Score: 318 bits (814), Expect = 1.32e-108
Identity = 160/177 (90.40%), Postives = 170/177 (96.05%), Query Frame = 0


Query: 2   NLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGRI 61
           N NL++LQYNVSKKKFLREPSRLFSF+DRQSSGLRRALQPSTEEIKQIFNKFD NRDGRI
Sbjct: 4   NPNLLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSTEEIKQIFNKFDRNRDGRI 63

Query: 62  SKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYAF 121
           SK EYRGILKALGR NSMEEVQ IFRAVD DGDG+INLNEFMEVHRSGGGVQARE+E+AF
Sbjct: 64  SKNEYRGILKALGRENSMEEVQNIFRAVDADGDGYINLNEFMEVHRSGGGVQAREIEFAF 123

Query: 122 RTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 178
           +TFDLNGDRKISAEEVMRVLRGLGE CS+EDCRRMVRAVD+DGDGMVDI+EFMTMMT
Sbjct: 124 KTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDNDGDGMVDINEFMTMMT 180

[Close]

BLAST of MC04g0533 vs. ExPASy TrEMBL
Match: A0A6J1C2T8 (calmodulin-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111007848 PE=4 SV=1)

HSP 1 Score: 348 bits (894), Expect = 3.92e-121
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   TNLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGR 60
           TNLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGR
Sbjct: 3   TNLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGR 62

Query: 61  ISKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYA 120
           ISKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYA
Sbjct: 63  ISKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYA 122

Query: 121 FRTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 178
           FRTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 123 FRTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

[Close]

BLAST of MC04g0533 vs. ExPASy TrEMBL
Match: A0A6J1JEH1 (calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111486169 PE=4 SV=1)

HSP 1 Score: 321 bits (822), Expect = 3.72e-110
Identity = 161/177 (90.96%), Postives = 171/177 (96.61%), Query Frame = 0


Query: 2   NLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGRI 61
           N NL++LQY VSKKKFLREPSRLFSF+DRQSSG+RRALQPSTEEIKQIFNKFDTNRDGRI
Sbjct: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGRI 63

Query: 62  SKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYAF 121
           SK EYRG+LKALGRGNSMEEVQKIFRAVD DGDG+INLNEFMEVHRSG GVQAREVE+AF
Sbjct: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQAREVEFAF 123

Query: 122 RTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 178
           +TFDLNGDRKISAEE+MRVLRGLGE CS+EDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

[Close]

BLAST of MC04g0533 vs. ExPASy TrEMBL
Match: A0A0A0K804 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073720 PE=4 SV=1)

HSP 1 Score: 320 bits (819), Expect = 1.06e-109
Identity = 160/177 (90.40%), Postives = 171/177 (96.61%), Query Frame = 0


Query: 2   NLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGRI 61
           N N + LQYNVSKKKFLREPSRLFSF+DRQSSGLRRALQPS+EEIKQIFNKFDTN+DGRI
Sbjct: 4   NPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRI 63

Query: 62  SKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYAF 121
           SK EYRGILKALGRGNSMEEVQKIFRAVD DGDG+INLNEFMEVHRSGGGVQA+EVE+AF
Sbjct: 64  SKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAF 123

Query: 122 RTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 178
           +TFDLNGDRKISAEEVMRVL+GLGE CS+EDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 124 KTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

[Close]

BLAST of MC04g0533 vs. ExPASy TrEMBL
Match: A0A5D3C643 (Calmodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00450 PE=4 SV=1)

HSP 1 Score: 317 bits (813), Expect = 8.75e-109
Identity = 159/177 (89.83%), Postives = 171/177 (96.61%), Query Frame = 0


Query: 2   NLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGRI 61
           N N ++LQYNVSKKKFLREPSRLFSF+DRQSSGLRRALQPS EEIKQIFNKFDTN+DGRI
Sbjct: 4   NPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKDGRI 63

Query: 62  SKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYAF 121
           SK EYRGILKALGRG+SMEEVQKIFRAVD DGDG+INLNEFMEVHRSGGGVQA+EVE+AF
Sbjct: 64  SKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAF 123

Query: 122 RTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 178
           +TFDLNGDRKISAEEVMRVL+GLGE CS+EDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 124 KTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

[Close]

BLAST of MC04g0533 vs. ExPASy TrEMBL
Match: A0A1S3C0X9 (calmodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103495233 PE=4 SV=1)

HSP 1 Score: 317 bits (813), Expect = 8.75e-109
Identity = 159/177 (89.83%), Postives = 171/177 (96.61%), Query Frame = 0


Query: 2   NLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRALQPSTEEIKQIFNKFDTNRDGRI 61
           N N ++LQYNVSKKKFLREPSRLFSF+DRQSSGLRRALQPS EEIKQIFNKFDTN+DGRI
Sbjct: 4   NPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKDGRI 63

Query: 62  SKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAREVEYAF 121
           SK EYRGILKALGRG+SMEEVQKIFRAVD DGDG+INLNEFMEVHRSGGGVQA+EVE+AF
Sbjct: 64  SKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAF 123

Query: 122 RTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 178
           +TFDLNGDRKISAEEVMRVL+GLGE CS+EDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 124 KTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

[Close]

BLAST of MC04g0533 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 201.4 bits (511), Expect = 5.9e-52
Identity = 100/183 (54.64%), Postives = 143/183 (78.14%), Query Frame = 0


Query: 1   TNLNLMNLQYNVSKKKFLREPSRLFSFRDRQSSGLRRA-----LQPSTEEIKQIFNKFDT 60
           +N++ + LQY +SK K LR+PSR+FS RDRQSSGL         QPS  E++++F++FD 
Sbjct: 2   SNVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDL 61

Query: 61  NRDGRISKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFMEVHRSGGGVQAR 120
           ++DG+IS+ EY+ +L+ALG+  ++E+V KIF+AVD DGDGFI+  EF++ ++  GG+++ 
Sbjct: 62  DKDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSS 121

Query: 121 EVEYAFRTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSDGDGMVDIDEFMT 179
           ++  +F TFDLNGD KISAEEVM VL  LGE CSLEDC RMVRAVD+DGDG+V+++EF+ 
Sbjct: 122 DIRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIK 181

[Close]

BLAST of MC04g0533 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 120.9 bits (302), Expect = 1.0e-27
Identity = 62/150 (41.33%), Postives = 95/150 (63.33%), Query Frame = 0


Query: 32  SSGLRRALQPSTEEIKQIFNKFDTNRDGRISKQEYRGILKALGRGNSMEEVQKIFRAVDG 91
           S  + R    S E+IK++F +FD N DG+IS  E + ++ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 92  DGDGFINLNEFMEV----HRSGGGVQAREVEYAFRTFDLNGDRKISAEEVMRVLRGLGEN 151
           DG+GFI+L+EF+ +     +S      R+++ AF  +DL+ + +ISA E+  V++ LGE 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 152 CSLEDCRRMVRAVDSDGDGMVDIDEFMTMM 178
           CS++DC+RM+  VDSDGDG VD +EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of MC04g0533 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 120.6 bits (301), Expect = 1.3e-27
Identity = 62/148 (41.89%), Postives = 92/148 (62.16%), Query Frame = 0


Query: 35  LRRALQPSTE--EIKQIFNKFDTNRDGRISKQEYRGILKALGRGNSMEEVQKIFRAVDGD 94
           L++A    TE  E++ +F KFD N DG+IS +E   I+ +LG     EE++K    +D  
Sbjct: 25  LQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRK 84

Query: 95  GDGFINLNEFMEVHRSGGGVQ--AREVEYAFRTFDLNGDRKISAEEVMRVLRGLGENCSL 154
           GDG+IN  EF+E++  G         ++ AF  +D++G+  ISAEE+  VLR LG+ CS+
Sbjct: 85  GDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSI 144

Query: 155 EDCRRMVRAVDSDGDGMVDIDEFMTMMT 179
            +CR+M+  VD DGDG +D +EF  MMT
Sbjct: 145 AECRKMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of MC04g0533 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 119.8 bits (299), Expect = 2.3e-27
Identity = 62/152 (40.79%), Postives = 100/152 (65.79%), Query Frame = 0


Query: 33  SGLRRALQPSTEEIKQIFNKFDTNRDGRISKQEYRGILKALGRGNSMEEVQKIFRAVDGD 92
           +G+ R+   S ++IK++F +FD N DG+IS  E + +++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 93  GDGFINLNEFM---EVHRSGGGVQAREV---EYAFRTFDLNGDRKISAEEVMRVLRGLGE 152
           G+GFI+L+EF+   ++   GGG    +V   + AF  +DL+G+ +ISA+E+  V++ LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 153 NCSLEDCRRMVRAVDSDGDGMVDIDEFMTMMT 179
            CS++DC++M+  VD DGDG V+ DEF  MM+
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of MC04g0533 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 117.9 bits (294), Expect = 8.6e-27
Identity = 61/135 (45.19%), Postives = 89/135 (65.93%), Query Frame = 0


Query: 44  EEIKQIFNKFDTNRDGRISKQEYRGILKALGRGNSMEEVQKIFRAVDGDGDGFINLNEFM 103
           EE+K++F++FD+N DG+IS  E  G+ KA+G   +  E+ ++   VD D DG+INL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 104 EVHRSGGGVQAREVEYAFRTFDLNGDRKISAEEVMRVLRGLGENCSLEDCRRMVRAVDSD 163
            + RS     A E+  AF  +D + +  ISA E+ +VL  LG +CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 164 GDGMVDIDEFMTMMT 179
           GDG V+ +EF  MMT
Sbjct: 142 GDGNVNFEEFQKMMT 154

The following BLAST results are available for this feature:

BLAST of MC04g0533 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQE6	8.3e-51	54.64	Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1	[more]
Q8RZB5	4.9e-27	41.45	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9C8Y1	1.4e-26	41.33	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
Q9FYK2	1.9e-26	41.89	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
P25070	3.2e-26	40.79	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]

BLAST of MC04g0533 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136065.1	8.09e-121	100.00	calmodulin-like protein 1 [Momordica charantia]	[more]
XP_022988967.1	7.67e-110	90.96	calmodulin-like protein 1 [Cucurbita maxima]	[more]
XP_004137114.2	2.20e-109	90.40	calmodulin-like protein 30 [Cucumis sativus] >KGN43936.1 hypothetical protein Cs...	[more]
KAG6588656.1	2.20e-109	90.40	Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]
XP_038888181.1	1.32e-108	90.40	calmodulin-like protein 30 [Benincasa hispida]	[more]

BLAST of MC04g0533 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2T8	3.92e-121	100.00	calmodulin-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111007848 PE=4 SV...	[more]
A0A6J1JEH1	3.72e-110	90.96	calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111486169 PE=4 SV=1	[more]
A0A0A0K804	1.06e-109	90.40	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073720 PE=4 SV=1	[more]
A0A5D3C643	8.75e-109	89.83	Calmodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3C0X9	8.75e-109	89.83	calmodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103495233 PE=4 SV=1	[more]

BLAST of MC04g0533 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15680.1	5.9e-52	54.64	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	1.0e-27	41.33	calmodulin like 23	[more]
AT1G24620.1	1.3e-27	41.89	EF hand calcium-binding protein family	[more]
AT5G37770.1	2.3e-27	40.79	EF hand calcium-binding protein family	[more]
AT1G18210.1	8.6e-27	45.19	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 45..73 e-value: 2.5E-7 score: 40.3 coord: 81..109 e-value: 2.1E-4 score: 30.6 coord: 152..178 e-value: 6.6 score: 11.1 coord: 116..144 e-value: 0.0036 score: 26.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 117..177 e-value: 2.1E-12 score: 47.3 coord: 44..104 e-value: 2.1E-13 score: 50.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 148..178 score: 13.537066
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 41..76 score: 16.047653
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 116..147 score: 12.393354
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 77..112 score: 14.011288
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 45..107 e-value: 4.94677E-19 score: 74.5065
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 116..178 e-value: 2.82166E-18 score: 72.5805
None	No IPR available	GENE3D	1.10.238.10		coord: 4..109 e-value: 3.9E-23 score: 84.3
None	No IPR available	GENE3D	1.10.238.10		coord: 112..178 e-value: 1.4E-20 score: 75.2
None	No IPR available	PANTHER	PTHR10891:SF685	CALCIUM-BINDING PROTEIN CML34-RELATED	coord: 4..178
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 4..178
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 125..137
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 161..173
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 54..66
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 90..102
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 43..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0533.1	MC04g0533.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005509	calcium ion binding