MC04g0517 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0517
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	MC04: 4353326 .. 4355246 (+)
RNA-Seq Expression	MC04g0517
Synteny	MC04g0517

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGCTTTAAAATGGTGACAATGGCAACCACTAGTTACGATTTGATAGAATCTCAGCCATGGGAGAGGCCTTGGCCTTCGATCCTGCATTCATCCAAGCCATCGAACACCGGCCGGAGCTCGAACCCATCGATACCGTCGACGAAGTTCCAACCATAGACCTCTCCGTCTCTGATTCTCGCAGTATCGAACAGCTCGTTTCGGAGATAGCCTCTGCCTGTGAGAAGTGGGGTTTCTTCCAGGTGATCAACCATGGAGTTCCCGTCGAGTTGCACGCGAGACTTGATAAAGCAGCGAAGTCGTTTTTCGATCAAACAGTGGAGGAGAAGAGGAACGTGAAGAGAGATGCAGCGAATGCCATGGGGTTCCACGATGCTGAGAATACGAAAAACGTCAGGGATTGGAAGGAGGTGTTTGATTTCTTGATTCGGGATCGGACGTTGATTCCTGCCTCGCACGAGCCAGATGATACGAAATTGAGGGTATTGTCCAATCAGTGGCCCCAATATCCCCCTGGGTTCAGGTACTTACTTTTCGGTCGAATTTGTTCCTTGTCTTAAAGCATCTACTTAACAGATTCAGATTCCTCGCCTGTATTAGTTTCCTTCGATAATCTAGTTCATGTTTCTTAATTGGATTACTTGAAAGAGAATTGAGGCGTTTGTTATTGGGTTGGAGGAACTGGCACACCAACAGTTCAACTAAATTCCCCTGTGAAACCTTCAGCTTGCATTTCTTCATCTTCTGCTGGAAAAACTTGTTATACTTAGCTTACGGTTCCTGGGAAATGGTGTGTTGGTTTTCTTGAACAGGGAAACTTGCGAGGAGTATGCAAGAGAATTGGAATTGTTAGCTGATAAGCTACTGGGGCTAATTTGTTTGAGTCTAGGCTTACCTGGAGCTGAAGGATTGAGGGGCTATTTCAAAGACGACCAAACGAGCGTGGTTAGGCTGAATCACTATCCTCCATGCCCGGCTCCGAATCTCGTGCTTGGCGTTGGGCATCACAAGGATGCCGGTGCCTTGACAATCCTTGCCCAGGATGGTGTGGGAGGACTGCAAGTGAAGAGAAAATCAGATGGAGAGTGGATTCCTGTCAAACCAATCCCAAATGCATATATTGTTAACATTGGTGATATTGTTCAAGTAAGTTTCTGTTCTAGGAATCGATATACAATTTACTTATGTCTTCTTCGGCCATGGTTATGTGAACATGATGCAATTAAGCAAAAATGACCTCTGATACTTGTGTGTGGGTAGCATTGATTACGAACGATTAACTTAGTCTAAAGAGTTTGTTTTCCGAAGGAGGATTGACAGACGTGTCGAGGTTTGGCTATACCGCAAGCACTCGCTGCTCTTGTACAGTGGGAGAGAACATTTTCCATTGTGTTCATAAAAGCTTCCAAGGGTAGCAAACTGGGATTTCAAACTCTTTTACCCCTCTGTATCCAGTTGTTTCATTAGAGTACACTTGTAAAACTTCCTTACTTTCTCCAAGAGCTAATAGCAGAGAACCATGATTTGGTTACTAGAACTCTTTAGCTTCCACTTCCGACTGAAATGCTTTTTCCACAATTCCTTAGACTCTTCGCCGAAAACCGAAGATTTCTTCTTGCCATCGACGAATGTGGTGTGGACAAAGTATGATCTGAATTGATGTTGTATGTGCAGGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGGGCGGTGGTGAATACTGAGAAGGAAAGATTTTCCTTTCCATTCTTCTTCTTTCCAGCGCACCATATGATGGTGAAGCCATTGGAGGAGCTTGTGAATGATCAAAATCCCTCAAAATACAGGGAATACAACTGGGGGAAGTTCTTTTGCACCAGAAACCGCAGTGATTACCAGAAGCAAAAAGTGGAAAACATTCAAATTGACCATTTTAGG

mRNA sequence

CAGCTTTAAAATGGTGACAATGGCAACCACTAGTTACGATTTGATAGAATCTCAGCCATGGGAGAGGCCTTGGCCTTCGATCCTGCATTCATCCAAGCCATCGAACACCGGCCGGAGCTCGAACCCATCGATACCGTCGACGAAGTTCCAACCATAGACCTCTCCGTCTCTGATTCTCGCAGTATCGAACAGCTCGTTTCGGAGATAGCCTCTGCCTGTGAGAAGTGGGGTTTCTTCCAGGTGATCAACCATGGAGTTCCCGTCGAGTTGCACGCGAGACTTGATAAAGCAGCGAAGTCGTTTTTCGATCAAACAGTGGAGGAGAAGAGGAACGTGAAGAGAGATGCAGCGAATGCCATGGGGTTCCACGATGCTGAGAATACGAAAAACGTCAGGGATTGGAAGGAGGTGTTTGATTTCTTGATTCGGGATCGGACGTTGATTCCTGCCTCGCACGAGCCAGATGATACGAAATTGAGGGTATTGTCCAATCAGTGGCCCCAATATCCCCCTGGGTTCAGGGAAACTTGCGAGGAGTATGCAAGAGAATTGGAATTGTTAGCTGATAAGCTACTGGGGCTAATTTGTTTGAGTCTAGGCTTACCTGGAGCTGAAGGATTGAGGGGCTATTTCAAAGACGACCAAACGAGCGTGGTTAGGCTGAATCACTATCCTCCATGCCCGGCTCCGAATCTCGTGCTTGGCGTTGGGCATCACAAGGATGCCGGTGCCTTGACAATCCTTGCCCAGGATGGTGTGGGAGGACTGCAAGTGAAGAGAAAATCAGATGGAGAGTGGATTCCTGTCAAACCAATCCCAAATGCATATATTGTTAACATTGGTGATATTGTTCAAGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGGGCGGTGGTGAATACTGAGAAGGAAAGATTTTCCTTTCCATTCTTCTTCTTTCCAGCGCACCATATGATGGTGAAGCCATTGGAGGAGCTTGTGAATGATCAAAATCCCTCAAAATACAGGGAATACAACTGGGGGAAGTTCTTTTGCACCAGAAACCGCAGTGATTACCAGAAGCAAAAAGTGGAAAACATTCAAATTGACCATTTTAGG

Coding sequence (CDS)

ATGGGAGAGGCCTTGGCCTTCGATCCTGCATTCATCCAAGCCATCGAACACCGGCCGGAGCTCGAACCCATCGATACCGTCGACGAAGTTCCAACCATAGACCTCTCCGTCTCTGATTCTCGCAGTATCGAACAGCTCGTTTCGGAGATAGCCTCTGCCTGTGAGAAGTGGGGTTTCTTCCAGGTGATCAACCATGGAGTTCCCGTCGAGTTGCACGCGAGACTTGATAAAGCAGCGAAGTCGTTTTTCGATCAAACAGTGGAGGAGAAGAGGAACGTGAAGAGAGATGCAGCGAATGCCATGGGGTTCCACGATGCTGAGAATACGAAAAACGTCAGGGATTGGAAGGAGGTGTTTGATTTCTTGATTCGGGATCGGACGTTGATTCCTGCCTCGCACGAGCCAGATGATACGAAATTGAGGGTATTGTCCAATCAGTGGCCCCAATATCCCCCTGGGTTCAGGGAAACTTGCGAGGAGTATGCAAGAGAATTGGAATTGTTAGCTGATAAGCTACTGGGGCTAATTTGTTTGAGTCTAGGCTTACCTGGAGCTGAAGGATTGAGGGGCTATTTCAAAGACGACCAAACGAGCGTGGTTAGGCTGAATCACTATCCTCCATGCCCGGCTCCGAATCTCGTGCTTGGCGTTGGGCATCACAAGGATGCCGGTGCCTTGACAATCCTTGCCCAGGATGGTGTGGGAGGACTGCAAGTGAAGAGAAAATCAGATGGAGAGTGGATTCCTGTCAAACCAATCCCAAATGCATATATTGTTAACATTGGTGATATTGTTCAAGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGGGCGGTGGTGAATACTGAGAAGGAAAGATTTTCCTTTCCATTCTTCTTCTTTCCAGCGCACCATATGATGGTGAAGCCATTGGAGGAGCTTGTGAATGATCAAAATCCCTCAAAATACAGGGAATACAACTGGGGGAAGTTCTTTTGCACCAGAAACCGCAGTGATTACCAGAAGCAAAAAGTGGAAAACATTCAAATTGACCATTTTAGG

Protein sequence

MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFFQVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFDFLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLGLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVKRKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHHMMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR

Homology

[Close]

BLAST of MC04g0517 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 7.8e-53
Identity = 125/325 (38.46%), Postives = 180/325 (55.38%), Query Frame = 0


Query: 11  FIQAIEHRPELEPI-DTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFFQVINHGVPV 70
           +I++   +P +  +   V EVP IDLS SD + I  LVSE   A ++WG FQV+NHG+P 
Sbjct: 23  YIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLVSE---ASKEWGIFQVVNHGIPN 82

Query: 71  ELHARLDKAAKSFFDQTVEEKRNV-KRDAANAMGFHDAENTKNVRDWKEVFDFLIRDRTL 130
           E+  +L +  K FF+   EEK  + K + + ++  +     K V   K   D L     +
Sbjct: 83  EVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQKEVDGKKGWVDHLF--HKI 142

Query: 131 IPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLGLPGAEGL 190
            P S         +    WP+ PP +RE  EEYA+ L+L+ D L   + L L L      
Sbjct: 143 WPPS--------AINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLGLDLEPNSFK 202

Query: 191 RGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVKRKSDGEWI 250
            G   DD   ++++N+YPPCP P+L LGV  H D  A+T+L  + V GLQV +  DG W 
Sbjct: 203 DGAGGDDLVYLMKINYYPPCPRPDLALGVA-HTDMSAITVLVPNEVPGLQVYK--DGHWY 262

Query: 251 PVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHHMMVKPLEE 310
             K IPNA IV+IGD V++ SN KY+SV HR  VN EK R S+P F  P     V P+ +
Sbjct: 263 DCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDHEVGPIPK 322

Query: 311 LVNDQNPSKYREYNWGKF-FCTRNR 333
           LVN++NP+K++   +  + +C  N+
Sbjct: 323 LVNEENPAKFKTKKYKDYAYCKLNK 331

[Close]

BLAST of MC04g0517 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 6.6e-52
Identity = 125/324 (38.58%), Postives = 173/324 (53.40%), Query Frame = 0


Query: 18  RPELE-PIDT-----VDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFFQVINHGVPVEL 77
           RPE E P  T       E+PTIDL   D    ++LV  IA A  +WG FQV NHG+P +L
Sbjct: 25  RPEKEQPASTTYHGPAPEIPTIDL---DDPVQDRLVRSIAEASREWGIFQVTNHGIPSDL 84

Query: 78  HARLDKAAKSFFDQTVEEKRNVKR--DAANAMGFHDAENTKNVRDWKEVFDFLIRDRTLI 137
             +L    K FF+   EEK    R  DA +  G+   +  K V   K   D L   R   
Sbjct: 85  ICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGY-GTKLQKEVEGKKSWVDHLFH-RVWP 144

Query: 138 PASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLGLPGAEGLR 197
           P+S         +    WP+ PP +R   EEYA+ +  + DKL   + L LG+ G     
Sbjct: 145 PSS---------INYRFWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLGLGVEGGVLKE 204

Query: 198 GYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVKRKSDGEWIP 257
               DD   ++++N+YPPCP P+L LGV  H D  ALT+L  + V GLQV +  D  WI 
Sbjct: 205 AAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFK--DDRWID 264

Query: 258 VKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHHMMVKPLEEL 317
            K IPNA +++IGD +++ SN KY++V HR  VN +K R S+P F  P    +V PL +L
Sbjct: 265 AKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADTVVGPLPQL 324

Query: 318 VNDQNPSKYREYNWGKF-FCTRNR 333
           V+D+NP KY+   +  + +C  N+
Sbjct: 325 VDDENPPKYKAKKFKDYSYCKLNK 332

[Close]

BLAST of MC04g0517 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 8.6e-52
Identity = 117/326 (35.89%), Postives = 175/326 (53.68%), Query Frame = 0


Query: 11  FIQAIEHRPELEPIDTV-DEVPTIDLSVSDSRSIEQLVSEIASACEKWGFFQVINHGVPV 70
           +++ +  RP++  + T  D +P IDL      +   ++++ A AC   GFFQ+ NHGVP 
Sbjct: 22  YVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQIKNHGVPE 81

Query: 71  ELHARLDKAAKSFFDQTVEEK-----RNVKRDAANAMGFH-DAENTKNVRDWKEVFDFLI 130
           E   ++  AA+ FF Q+  E+      + K+    +  F+   E   N RD+  +  + I
Sbjct: 82  ETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPI 141

Query: 131 RDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLGLP 190
            D                   N+WP  P  FRE   EYA  +  L   LL  I  SLGL 
Sbjct: 142 ED-----------------FINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLA 201

Query: 191 GAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVKRKS 250
                    K  Q   + +N+YP CP P L  G+  HKDA  +T+L QD V GLQV +  
Sbjct: 202 KDRVSNTIGKHGQH--MAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFK-- 261

Query: 251 DGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHHMMV 310
           DG+WI V P+PN +IVN+GD +QV SN+KY+SV HRAVVN++ ER S P F+ P+   ++
Sbjct: 262 DGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVI 321

Query: 311 KPLEELVNDQ--NPSKYREYNWGKFF 328
            P +EL+N++  +P+ YR + + ++F
Sbjct: 322 SPAQELINEEEDSPAIYRNFTYAEYF 326

[Close]

BLAST of MC04g0517 vs. ExPASy Swiss-Prot
Match: Q7XZQ6 (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 4.3e-51
Identity = 119/325 (36.62%), Postives = 177/325 (54.46%), Query Frame = 0


Query: 11  FIQAIEHRPELEPID-TVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFFQVINHGVPV 70
           +I++   +P +  +   V +VP IDL  S + + E LV  IA A  +WG FQV+NHG+P 
Sbjct: 23  YIRSESEQPAVTTMQGVVLQVPVIDLG-SSNNTEENLVELIAEASREWGIFQVVNHGIPD 82

Query: 71  ELHARLDKAAKSFFDQTVEEKRNV-KRDAANAMGFHDAENTKNVRDWKEVFDFLIRDRTL 130
           +  A+L K  K FF+   +EK  + K +    +  +  +  K +   K   D L     +
Sbjct: 83  DAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTKLQKELGGKKGWVDHLF--HII 142

Query: 131 IPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLGLPGAEGL 190
            P        K  V  N WP  PP +RE  EEYA  L  + DKL   + L +GL   E  
Sbjct: 143 WP--------KSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALSLGIGLEKHELK 202

Query: 191 RGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVKRKSDGEWI 250
           +    DD   ++++N+YPPCP P+L LGV  H D  A+TIL  + V GLQV +  D  W 
Sbjct: 203 KASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGLQVHK--DDHWY 262

Query: 251 PVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHHMMVKPLEE 310
            VK IPNA I++IGD +++ SN KY+SV HR  VN +K R S+P F  P   ++  P+ +
Sbjct: 263 DVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPPELLTGPISK 322

Query: 311 LVNDQNPSKYREYNWGKF-FCTRNR 333
           L+ D+NP+K++   +  + +C  N+
Sbjct: 323 LITDENPAKFKTKKYKDYVYCKLNK 334

[Close]

BLAST of MC04g0517 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 9.5e-51
Identity = 131/337 (38.87%), Postives = 176/337 (52.23%), Query Frame = 0


Query: 11  FIQAIEHRPELEPIDTV-DEVPTIDLSVSDSRSIEQLVSEIASACEKWGFFQVINHGVPV 70
           +++ I  RP L  +++  D +P IDL      +   +V ++ASAC  +GFFQ+ NHGVP 
Sbjct: 24  YVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNHGVPD 83

Query: 71  ELHARLDKAAKSFFDQTVEEKRNVKRDAAN-------AMGFH-DAENTKNVRDWKEVFDF 130
               ++   A+ FF Q   E   VK  +A+       +  F+  A+   N RD+  +  F
Sbjct: 84  TTVNKMQTVAREFFHQ--PESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLHCF 143

Query: 131 LIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLG 190
            I D                    +WP  P  FRE   EYA  +  L  +LL  I  SLG
Sbjct: 144 PIED-----------------FIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLG 203

Query: 191 LPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVKR 250
           L          K  Q   +  N+YPPCP P L  G+  HKD   +T+L QD V GLQV +
Sbjct: 204 LESDHISNILGKHAQH--MAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFK 263

Query: 251 KSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHHM 310
             D +W+ V PIPN +IVNIGD +QV SNDKY+SV HRAVVNTE ER S P F+FP+   
Sbjct: 264 --DDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDA 323

Query: 311 MVKPLEELVNDQNP-SKYREY----NWGKFFCTRNRS 334
           ++ P  ELVN+Q+  + YR Y     W KF+   NRS
Sbjct: 324 VIGPAHELVNEQDSLAIYRTYPFVEYWDKFW---NRS 334

[Close]

BLAST of MC04g0517 vs. NCBI nr
Match: XP_022135969.1 (flavonol synthase/flavanone 3-hydroxylase-like [Momordica charantia])

HSP 1 Score: 722 bits (1863), Expect = 7.40e-263
Identity = 348/349 (99.71%), Postives = 349/349 (100.00%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60
           MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF
Sbjct: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60

Query: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120
           QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD
Sbjct: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120

Query: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180
           FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL
Sbjct: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180

Query: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240
           GLPGAEGLRGYFKDDQTSVVRLNHYPPCP+PNLVLGVGHHKDAGALTILAQDGVGGLQVK
Sbjct: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPSPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240

Query: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300
           RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH
Sbjct: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300

Query: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349
           MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR
Sbjct: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349

[Close]

BLAST of MC04g0517 vs. NCBI nr
Match: XP_022135970.1 (flavonol synthase/flavanone 3-hydroxylase-like [Momordica charantia])

HSP 1 Score: 638 bits (1645), Expect = 1.21e-229
Identity = 309/349 (88.54%), Postives = 325/349 (93.12%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60
           MGEALAFDPAFIQ IEHRPELEPID+VDEVPTIDLSVSDSRSIEQLVSEI+SACEKWGFF
Sbjct: 1   MGEALAFDPAFIQPIEHRPELEPIDSVDEVPTIDLSVSDSRSIEQLVSEISSACEKWGFF 60

Query: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120
           QVINHGVPV+LHARL+KAAKSFFDQTVEEKR VKRDAANAMGFHDAENTKNVRDWKEVFD
Sbjct: 61  QVINHGVPVDLHARLEKAAKSFFDQTVEEKRKVKRDAANAMGFHDAENTKNVRDWKEVFD 120

Query: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180
           FLIR RTLIPASHEPDDTKLRVL+NQWPQYPPGFRETCEEYA+ELELLA KLLGL+ LSL
Sbjct: 121 FLIRGRTLIPASHEPDDTKLRVLANQWPQYPPGFRETCEEYAKELELLAHKLLGLVSLSL 180

Query: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240
           GLP A+ L+ YFKDDQ ++VRLNHYP CP P LVLGVG HKD  ALTILAQ  VGGLQVK
Sbjct: 181 GLP-ADRLKEYFKDDQLTIVRLNHYPLCPFPELVLGVGRHKDPSALTILAQGAVGGLQVK 240

Query: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300
           RKSDGEWIPVKP PNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPF  +P HH
Sbjct: 241 RKSDGEWIPVKPNPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFLLYPPHH 300

Query: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349
           MMVKPLEELVN+QNP KYREYNWGKF CTRNRSD++KQ+VENIQI HFR
Sbjct: 301 MMVKPLEELVNEQNPPKYREYNWGKFLCTRNRSDFKKQQVENIQIHHFR 348

[Close]

BLAST of MC04g0517 vs. NCBI nr
Match: XP_022931615.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 621 bits (1602), Expect = 4.46e-223
Identity = 292/349 (83.67%), Postives = 324/349 (92.84%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60
           MGEALAFD +FIQ I+HRP+LEP+D +DEVPTIDLS+SDSRS+EQLVS+IASACE WGFF
Sbjct: 1   MGEALAFDASFIQPIQHRPKLEPVDPIDEVPTIDLSISDSRSVEQLVSDIASACENWGFF 60

Query: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120
           QVINHGVP+EL AR+++  K FFDQ VEEKR VKRDAANAMG+HD ENTKNVRDWKEVFD
Sbjct: 61  QVINHGVPMELVARMEEVTKLFFDQGVEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFD 120

Query: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180
           FL++DRTL+PASHEPDDT LRVL+NQWPQYPPGFRETCEEYA+ELELLA KLLGLICLSL
Sbjct: 121 FLVKDRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLSL 180

Query: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240
           GLP A+ L+GYF+D+Q S+VRLN YPPC +P+LVLGVG HKDAGALTILAQDGVGGLQV+
Sbjct: 181 GLPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVR 240

Query: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300
           RKSDGEWIPVKPI NAYIVNIGDIVQVWSNDKYESVEHR VVNTEKERFS PFFFFPAHH
Sbjct: 241 RKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHH 300

Query: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349
            MVKPLEE+VN+QNP KYREYN+GKFF +RNRSD+QKQKVEN+QIDHFR
Sbjct: 301 TMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFR 349

[Close]

BLAST of MC04g0517 vs. NCBI nr
Match: KAG7022434.1 (Protein DMR6-LIKE OXYGENASE 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617 bits (1592), Expect = 1.49e-221
Identity = 290/349 (83.09%), Postives = 323/349 (92.55%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60
           MGEALAFD +FIQ I+HRP+LEP+D +DEVPTIDLS+SDSRS+EQLVS+IASACE WGFF
Sbjct: 1   MGEALAFDASFIQPIQHRPKLEPVDPIDEVPTIDLSISDSRSVEQLVSDIASACENWGFF 60

Query: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120
           QVINHGVP+EL AR+++  K FFDQ V+EKR VKRDAANAMG+HD ENTKNVRDWKEVFD
Sbjct: 61  QVINHGVPMELVARMEEVTKLFFDQGVDEKRKVKRDAANAMGYHDGENTKNVRDWKEVFD 120

Query: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180
           FL++DRTL+PASHEPDDT LRVL+NQWPQYPPGFRETCEEYA+ELELLA KLLGLICLSL
Sbjct: 121 FLVKDRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLSL 180

Query: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240
           GL  A+ L+GYF+D+Q S+VRLN YPPC +P+LVLGVG HKDAGALTILAQDGVGGLQV+
Sbjct: 181 GLSVADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVR 240

Query: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300
           RKSDGEWIPVKPI NAYIVNIGDIVQVWSNDKYESVEHR VVNTEKERFS PFFFFPAHH
Sbjct: 241 RKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHH 300

Query: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349
            MVKPLEE+VN+QNP KYREYN+GKFF +RNRSD+QKQKVEN+QIDHFR
Sbjct: 301 TMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFR 349

[Close]

BLAST of MC04g0517 vs. NCBI nr
Match: XP_022988832.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 617 bits (1591), Expect = 2.12e-221
Identity = 292/349 (83.67%), Postives = 322/349 (92.26%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60
           MGEALAFD +FIQ IEHRP+LE +D +DEVPTIDLS+SDSRS+EQLVS+IASACE WGFF
Sbjct: 1   MGEALAFDASFIQPIEHRPKLEHVDPIDEVPTIDLSISDSRSVEQLVSDIASACENWGFF 60

Query: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120
           QVINHGVP+EL AR++K AK FFDQ +EEKR VKRDAANAMG+HD ENTKNVRDWKEVFD
Sbjct: 61  QVINHGVPMELLARMEKVAKLFFDQGIEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFD 120

Query: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180
           FL+RDRTL+PASHEPD T LRVL+NQWPQYPP FRETCEEYA+ELELLA KLLGLICLSL
Sbjct: 121 FLVRDRTLVPASHEPDGTSLRVLTNQWPQYPPEFRETCEEYAKELELLAYKLLGLICLSL 180

Query: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240
           GLP A+ L+GYF+D+Q S+VRLN YPPC +P+LVLGVG HKDAGALTILAQDGVGGLQV+
Sbjct: 181 GLPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVR 240

Query: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300
           RKSDGEWIPVKPI NAYIVNIGDIVQVWSNDKYESVEHR VVNTEKERFS PFFFFPAHH
Sbjct: 241 RKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHH 300

Query: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349
            MVKPLEE+VN+QNP KYREYN+GKFF +RNRSD+QKQKVEN+QIDHFR
Sbjct: 301 TMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFR 349

[Close]

BLAST of MC04g0517 vs. ExPASy TrEMBL
Match: A0A6J1C2Z2 (flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111007793 PE=3 SV=1)

HSP 1 Score: 722 bits (1863), Expect = 3.58e-263
Identity = 348/349 (99.71%), Postives = 349/349 (100.00%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60
           MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF
Sbjct: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60

Query: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120
           QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD
Sbjct: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120

Query: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180
           FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL
Sbjct: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180

Query: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240
           GLPGAEGLRGYFKDDQTSVVRLNHYPPCP+PNLVLGVGHHKDAGALTILAQDGVGGLQVK
Sbjct: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPSPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240

Query: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300
           RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH
Sbjct: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300

Query: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349
           MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR
Sbjct: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349

[Close]

BLAST of MC04g0517 vs. ExPASy TrEMBL
Match: A0A6J1C2J5 (flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111007794 PE=3 SV=1)

HSP 1 Score: 638 bits (1645), Expect = 5.84e-230
Identity = 309/349 (88.54%), Postives = 325/349 (93.12%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60
           MGEALAFDPAFIQ IEHRPELEPID+VDEVPTIDLSVSDSRSIEQLVSEI+SACEKWGFF
Sbjct: 1   MGEALAFDPAFIQPIEHRPELEPIDSVDEVPTIDLSVSDSRSIEQLVSEISSACEKWGFF 60

Query: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120
           QVINHGVPV+LHARL+KAAKSFFDQTVEEKR VKRDAANAMGFHDAENTKNVRDWKEVFD
Sbjct: 61  QVINHGVPVDLHARLEKAAKSFFDQTVEEKRKVKRDAANAMGFHDAENTKNVRDWKEVFD 120

Query: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180
           FLIR RTLIPASHEPDDTKLRVL+NQWPQYPPGFRETCEEYA+ELELLA KLLGL+ LSL
Sbjct: 121 FLIRGRTLIPASHEPDDTKLRVLANQWPQYPPGFRETCEEYAKELELLAHKLLGLVSLSL 180

Query: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240
           GLP A+ L+ YFKDDQ ++VRLNHYP CP P LVLGVG HKD  ALTILAQ  VGGLQVK
Sbjct: 181 GLP-ADRLKEYFKDDQLTIVRLNHYPLCPFPELVLGVGRHKDPSALTILAQGAVGGLQVK 240

Query: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300
           RKSDGEWIPVKP PNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPF  +P HH
Sbjct: 241 RKSDGEWIPVKPNPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFLLYPPHH 300

Query: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349
           MMVKPLEELVN+QNP KYREYNWGKF CTRNRSD++KQ+VENIQI HFR
Sbjct: 301 MMVKPLEELVNEQNPPKYREYNWGKFLCTRNRSDFKKQQVENIQIHHFR 348

[Close]

BLAST of MC04g0517 vs. ExPASy TrEMBL
Match: A0A6J1EZZ4 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111437780 PE=3 SV=1)

HSP 1 Score: 621 bits (1602), Expect = 2.16e-223
Identity = 292/349 (83.67%), Postives = 324/349 (92.84%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60
           MGEALAFD +FIQ I+HRP+LEP+D +DEVPTIDLS+SDSRS+EQLVS+IASACE WGFF
Sbjct: 1   MGEALAFDASFIQPIQHRPKLEPVDPIDEVPTIDLSISDSRSVEQLVSDIASACENWGFF 60

Query: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120
           QVINHGVP+EL AR+++  K FFDQ VEEKR VKRDAANAMG+HD ENTKNVRDWKEVFD
Sbjct: 61  QVINHGVPMELVARMEEVTKLFFDQGVEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFD 120

Query: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180
           FL++DRTL+PASHEPDDT LRVL+NQWPQYPPGFRETCEEYA+ELELLA KLLGLICLSL
Sbjct: 121 FLVKDRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLSL 180

Query: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240
           GLP A+ L+GYF+D+Q S+VRLN YPPC +P+LVLGVG HKDAGALTILAQDGVGGLQV+
Sbjct: 181 GLPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVR 240

Query: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300
           RKSDGEWIPVKPI NAYIVNIGDIVQVWSNDKYESVEHR VVNTEKERFS PFFFFPAHH
Sbjct: 241 RKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHH 300

Query: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349
            MVKPLEE+VN+QNP KYREYN+GKFF +RNRSD+QKQKVEN+QIDHFR
Sbjct: 301 TMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFR 349

[Close]

BLAST of MC04g0517 vs. ExPASy TrEMBL
Match: A0A6J1JE50 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111486062 PE=3 SV=1)

HSP 1 Score: 617 bits (1591), Expect = 1.02e-221
Identity = 292/349 (83.67%), Postives = 322/349 (92.26%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60
           MGEALAFD +FIQ IEHRP+LE +D +DEVPTIDLS+SDSRS+EQLVS+IASACE WGFF
Sbjct: 1   MGEALAFDASFIQPIEHRPKLEHVDPIDEVPTIDLSISDSRSVEQLVSDIASACENWGFF 60

Query: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120
           QVINHGVP+EL AR++K AK FFDQ +EEKR VKRDAANAMG+HD ENTKNVRDWKEVFD
Sbjct: 61  QVINHGVPMELLARMEKVAKLFFDQGIEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFD 120

Query: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180
           FL+RDRTL+PASHEPD T LRVL+NQWPQYPP FRETCEEYA+ELELLA KLLGLICLSL
Sbjct: 121 FLVRDRTLVPASHEPDGTSLRVLTNQWPQYPPEFRETCEEYAKELELLAYKLLGLICLSL 180

Query: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240
           GLP A+ L+GYF+D+Q S+VRLN YPPC +P+LVLGVG HKDAGALTILAQDGVGGLQV+
Sbjct: 181 GLPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVR 240

Query: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300
           RKSDGEWIPVKPI NAYIVNIGDIVQVWSNDKYESVEHR VVNTEKERFS PFFFFPAHH
Sbjct: 241 RKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHH 300

Query: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349
            MVKPLEE+VN+QNP KYREYN+GKFF +RNRSD+QKQKVEN+QIDHFR
Sbjct: 301 TMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFR 349

[Close]

BLAST of MC04g0517 vs. ExPASy TrEMBL
Match: A0A6J1C6B7 (flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111007792 PE=3 SV=1)

HSP 1 Score: 602 bits (1551), Expect = 1.27e-215
Identity = 287/349 (82.23%), Postives = 314/349 (89.97%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDTVDEVPTIDLSVSDSRSIEQLVSEIASACEKWGFF 60
           MGE LAF PAF++ IEHRP+LEPIDTVDEVPTIDLSVS+SRSIEQLVSEIASACEKWG F
Sbjct: 1   MGETLAFHPAFVRDIEHRPKLEPIDTVDEVPTIDLSVSESRSIEQLVSEIASACEKWGSF 60

Query: 61  QVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFD 120
           QVINHGVPVEL  RL KAAK FFDQTVEEKR VKRD+   +GF+D ENT+NVRDWKEVFD
Sbjct: 61  QVINHGVPVELIVRLAKAAKLFFDQTVEEKRKVKRDSETGLGFYDGENTENVRDWKEVFD 120

Query: 121 FLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSL 180
           FL++DR   PASHEPDDTKLRVLSNQWPQYPP FRETCEEYARELELLA  LLGLICLSL
Sbjct: 121 FLVQDRAWTPASHEPDDTKLRVLSNQWPQYPPWFRETCEEYARELELLAHNLLGLICLSL 180

Query: 181 GLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVK 240
           GLP AEGLRGYF+D++ S VRLNHYPPCP+P+LVLG+  HKD GALTILAQD VGGLQVK
Sbjct: 181 GLPCAEGLRGYFQDEKMSTVRLNHYPPCPSPDLVLGIDPHKDPGALTILAQDDVGGLQVK 240

Query: 241 RKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHH 300
           RKSDGEWIPVKPIPNAYIVNIG+I+QVWSNDKYESVEHRAVVN+EKERFSFPF F PAHH
Sbjct: 241 RKSDGEWIPVKPIPNAYIVNIGEIIQVWSNDKYESVEHRAVVNSEKERFSFPFCFLPAHH 300

Query: 301 MMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 349
           MMVKP EELVN+QNP KYREYNWGK+FC R RS +++Q+VE+IQ+  FR
Sbjct: 301 MMVKPWEELVNEQNPPKYREYNWGKYFCMRKRSHFKEQEVEDIQVHRFR 349

[Close]

BLAST of MC04g0517 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 459.1 bits (1180), Expect = 3.1e-129
Identity = 217/356 (60.96%), Postives = 274/356 (76.97%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDT-----VDEVPTIDLSVSDSRSIEQ--LVSEIASA 60
           MGE    D AFIQA EHRP     ++      DE+PTIDLS  +    ++  +  EIA A
Sbjct: 1   MGE---LDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEA 60

Query: 61  CEKWGFFQVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVR 120
           C++WGFFQVINHG+P  L  R++K A  FF+ T EEKR VKRD  N MG+HD E+TKNVR
Sbjct: 61  CKRWGFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVR 120

Query: 121 DWKEVFDFLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLL 180
           DWKE+FDF ++D T++PAS EP+DT+LR L+NQWPQ P  FRE C+EYARE+E LA +LL
Sbjct: 121 DWKEIFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLL 180

Query: 181 GLICLSLGLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDG 240
            L+ +SLGLPG + L G+F ++QTS +R NHYPPCP P L LGVG HKD GALT+LAQD 
Sbjct: 181 ELVSISLGLPG-DRLTGFF-NEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDS 240

Query: 241 VGGLQVKRKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPF 300
           VGGLQV R+SDG+WIPVKPI +A I+N+G+ +QVW+ND+Y S EHR VVNT KERFS PF
Sbjct: 241 VGGLQVSRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPF 300

Query: 301 FFFPAHHMMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 350
           FFFP+H   ++PLEEL++++NP  Y++YNWGKFF +RNRSD++K +VENIQIDHF+
Sbjct: 301 FFFPSHEANIEPLEELISEENPPCYKKYNWGKFFVSRNRSDFKKLEVENIQIDHFK 351

[Close]

BLAST of MC04g0517 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 421.4 bits (1082), Expect = 7.1e-118
Identity = 195/344 (56.69%), Postives = 254/344 (73.84%), Query Frame = 0


Query: 8   DPAFIQAIEHRPELEPI-DTVDEVPTIDLS-VSDSRSIEQLVSEIASACEKWGFFQVINH 67
           DP +IQA EHR +   I +  +E+P IDLS + D   ++ ++SEI  ACEKWGFFQV+NH
Sbjct: 5   DPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLNH 64

Query: 68  GVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFDFLIRD 127
           GVP +   R++K  K FFD  +EEK  VKRD  N +G+HD E+TKNV+DWKEVFD   +D
Sbjct: 65  GVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFKD 124

Query: 128 RTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLGLPGA 187
             +IP++ +P+D  LR++ N+WPQ P  FRE CE YAR  E LA KLL LI LSLGLP  
Sbjct: 125 PMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLP-K 184

Query: 188 EGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVKRKSDG 247
           E    YFK +Q S  R+N YPPCP P+L LGVGHHKDA  +++LAQD VGGLQV R+SDG
Sbjct: 185 ERFHDYFK-EQMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDG 244

Query: 248 EWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHHMMVKP 307
            W P++P+PNA ++NIG+ +++W+NDKY S EHR VVNT +ER+S PFF  P+H + VKP
Sbjct: 245 VWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKP 304

Query: 308 LEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 350
           LEELV+ +NP KY+ Y WGKF+ +RNRSD++K ++ENIQID F+
Sbjct: 305 LEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQIDDFK 346

[Close]

BLAST of MC04g0517 vs. TAIR 10
Match: AT3G19010.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 3.7e-106
Identity = 177/344 (51.45%), Postives = 235/344 (68.31%), Query Frame = 0


Query: 8   DPAFIQAIEHRPELEPI-DTVDEVPTIDLS-VSDSRSIEQLVSEIASACEKWGFFQVINH 67
           DP +IQA EHR +   I +  +E+P IDLS + D   ++ ++SEI  ACEKWGFFQV+NH
Sbjct: 5   DPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLNH 64

Query: 68  GVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFDFLIRD 127
           GVP +   R++K  K FFD  +EEK  VKRD  N +G+HD E+TKNV+DWKEVFD   +D
Sbjct: 65  GVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFKD 124

Query: 128 RTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLGLPGA 187
             +IP++ +P+D  LR++ N+WPQ P  FRE CE YAR  E LA                
Sbjct: 125 PMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLA---------------- 184

Query: 188 EGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVKRKSDG 247
                           +N YPPCP P+L LGVGHHKDA  +++LAQD VGGLQV R+SDG
Sbjct: 185 ----------------INRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDG 244

Query: 248 EWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAHHMMVKP 307
            W P++P+PNA ++NIG+ +++W+NDKY S EHR VVNT +ER+S PFF  P+H + VKP
Sbjct: 245 VWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKP 304

Query: 308 LEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFR 350
           LEELV+ +NP KY+ Y WGKF+ +RNRSD++K ++ENIQID F+
Sbjct: 305 LEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQIDDFK 316

[Close]

BLAST of MC04g0517 vs. TAIR 10
Match: AT3G19000.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 1.9e-91
Identity = 163/274 (59.49%), Postives = 204/274 (74.45%), Query Frame = 0


Query: 1   MGEALAFDPAFIQAIEHRPELEPIDT-----VDEVPTIDLSVSDSRSIEQ--LVSEIASA 60
           MGE    D AFIQA EHRP     ++      DE+PTIDLS  +    ++  +  EIA A
Sbjct: 1   MGE---LDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEA 60

Query: 61  CEKWGFFQVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVR 120
           C++WGFFQVINHG+P  L  R++K A  FF+ T EEKR VKRD  N MG+HD E+TKNVR
Sbjct: 61  CKRWGFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVR 120

Query: 121 DWKEVFDFLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLL 180
           DWKE+FDF ++D T++PAS EP+DT+LR L+NQWPQ P  FRE C+EYARE+E LA +LL
Sbjct: 121 DWKEIFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLL 180

Query: 181 GLICLSLGLPGAEGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDG 240
            L+ +SLGLPG + L G+F ++QTS +R NHYPPCP P L LGVG HKD GALT+LAQD 
Sbjct: 181 ELVSISLGLPG-DRLTGFF-NEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDS 240

Query: 241 VGGLQVKRKSDGEWIPVKPIPNAYIVNIGDIVQV 268
           VGGLQV R+SDG+WIPVKPI +A I+N+G+ +QV
Sbjct: 241 VGGLQVSRRSDGQWIPVKPISDALIINMGNCIQV 269

[Close]

BLAST of MC04g0517 vs. TAIR 10
Match: AT3G19010.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 4.3e-83
Identity = 145/262 (55.34%), Postives = 188/262 (71.76%), Query Frame = 0


Query: 8   DPAFIQAIEHRPELEPI-DTVDEVPTIDLS-VSDSRSIEQLVSEIASACEKWGFFQVINH 67
           DP +IQA EHR +   I +  +E+P IDLS + D   ++ ++SEI  ACEKWGFFQV+NH
Sbjct: 5   DPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLNH 64

Query: 68  GVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVFDFLIRD 127
           GVP +   R++K  K FFD  +EEK  VKRD  N +G+HD E+TKNV+DWKEVFD   +D
Sbjct: 65  GVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFKD 124

Query: 128 RTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLSLGLPGA 187
             +IP++ +P+D  LR++ N+WPQ P  FRE CE YAR  E LA KLL LI LSLGLP  
Sbjct: 125 PMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLP-K 184

Query: 188 EGLRGYFKDDQTSVVRLNHYPPCPAPNLVLGVGHHKDAGALTILAQDGVGGLQVKRKSDG 247
           E    YFK +Q S  R+N YPPCP P+L LGVGHHKDA  +++LAQD VGGLQV R+SDG
Sbjct: 185 ERFHDYFK-EQMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDG 244

Query: 248 EWIPVKPIPNAYIVNIGDIVQV 268
            W P++P+PNA ++NIG+ ++V
Sbjct: 245 VWFPIRPVPNALVINIGNCMEV 264

The following BLAST results are available for this feature:

BLAST of MC04g0517 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M547	7.8e-53	38.46	Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian...	[more]
Q9ZWQ9	6.6e-52	38.58	Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ...	[more]
Q9ZSA7	8.6e-52	35.89	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]
Q7XZQ6	4.3e-51	36.62	Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS...	[more]
Q9ZSA8	9.5e-51	38.87	Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1	[more]

BLAST of MC04g0517 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135969.1	7.40e-263	99.71	flavonol synthase/flavanone 3-hydroxylase-like [Momordica charantia]	[more]
XP_022135970.1	1.21e-229	88.54	flavonol synthase/flavanone 3-hydroxylase-like [Momordica charantia]	[more]
XP_022931615.1	4.46e-223	83.67	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata]	[more]
KAG7022434.1	1.49e-221	83.09	Protein DMR6-LIKE OXYGENASE 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022988832.1	2.12e-221	83.67	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima]	[more]

BLAST of MC04g0517 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2Z2	3.58e-263	99.71	flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1C2J5	5.84e-230	88.54	flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1EZZ4	2.16e-223	83.67	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JE50	1.02e-221	83.67	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1C6B7	1.27e-215	82.23	flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN...	[more]

BLAST of MC04g0517 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19000.1	3.1e-129	60.96	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.1	7.1e-118	56.69	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.3	3.7e-106	51.45	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.2	1.9e-91	59.49	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.2	4.3e-83	55.34	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 285..303 score: 35.41 coord: 50..67 score: 50.51 coord: 220..241 score: 40.91
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 7..341
None	No IPR available	PANTHER	PTHR10209:SF727	DIRIGENT PROTEIN	coord: 7..341
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 17..336
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 198..297 e-value: 3.8E-29 score: 101.2
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 30..149 e-value: 5.6E-26 score: 91.6
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..337 e-value: 2.1E-104 score: 351.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 196..297 score: 13.715759

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0517.1	MC04g0517.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity