MC04g0488 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0488
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	PfkB domain-containing protein
Location	MC04: 4115897 .. 4121190 (+)
RNA-Seq Expression	MC04g0488
Synteny	MC04g0488

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTATTTTAATTCTCAAAAAGGAAAAGATGCCTTATTTATTTGGGTGTGAAATATTCAACAAGATCGACAAAATGAAAATGGAATCGATCGTCGTTGTCAACCCTGCAGGAAGAACGTAATTCGACGCTGGTCTACGGGTTTCACAGTTACAGACTTTCATAGCTCCACTGAAAACCTTGGAGATTGGTAACAATGGAGAGCAGTGCTCAGAGGCGTATAAATTTGATTCGCCGGCACCTTCTTCCGCCGCCATGTGAGTTCAACGACGCTCTTAATCTGGTGAGCGCATCTCTTCACTCTTACAGTCTCATTTCGTTCTCTCTTCAAGGTGCTTTCTAGCGGCGACACGCTTTCTGCGATCAATGGTGATATCGTAAGTGTATATATAATTTTAGGCGTTGACAAATGGTGGGAGCGAATTGCAAATTGGGGAATCAGAGCCTGTGGTTATTGGAGGTATGGTGCTGGACATTCATGCCATACCCTCCATCGCCGCCGTTCCGAGGTCAACTACTCCCGGCAAGGTAATTTACTAATTTTCTCTTGAAATATGAGAGGTTCATCGTGATCGCGATATGAATTTTATTTAAGGGCGAATTGTCACTGAGGTTGATCATCATTATCAGGTACTAGATTGATTGTTTACCTATTGGAGATATAGTACCTCCATGGTCACCTCTTAAGCTATGATCCTTGAAGTTTTTTAATGTTAAATGTTGTGGAATAATAGGGTTCTTTTAGAAGAAGCTGATCATATTCCTAATAGTTATATAAATCATTATAACTGTAAATTGCCCCTATGATTTGTTCAAGTAGGCGAGTTATCCAGTGCTTGTTTAGTTTCACTGCAAAATGTGGCATGGCAAACACATTCAAATATGAAAAATATTATTATCGAACAAGCTCAATTTTATATGAAAGTAAAAAATATTAGAAATGAATAGGTGTCATGTTTCTATTGTTGTCTTTCTTAGTGATTATTATCTTTAAAATTGTTTATGATATTGTATGTTAATTGATAACCATTTTAGCTTCAGTATATAATCTGAAATTACACTTGAAAATTATGTTTGAAAGATTTAGAGAAAATACTCGTCACTATGATATAGTTGAAATTATAACAACGACATGATTTGAATCTTCAAGGAATATGTTCCTATTGTGTATTTAACTTATTGCTTATGTTTATATTCAAGGATGGAAATAAATTTGACTGAACTAATTGTGTGGACTATGGAATATTCCTGGATTACCAAATCCTTGGCAATATATAATTCGTCCTAAATTCAACTATGATATGGCTAATAAGTTATTCTTAAATTTCATAGTTTTGCTTGTATGATAATGATAGAACTACCTTAAATCCTAATTTCATTGTGTTGGTATCTGGTCTTTTGTGGTTTGGCCAGTATCTCAATATTTTTCAATAGGCTGAACTCGTTGGTATCTGTAGCATCACAGTGACAATATTAGCGTAGAGAATCCATTTCTTAGTTTTCTATATTCGAAATTTTACCTTTGAGAATGTGGTGTTTTAGATCAACTATGTATTAGGAGGCGTAGCACGGAATGTTGCTGAATGCATGTCAAAGTTCGGGACCAAACTTTTCCTGATTAGTGTTGTGGGACATGACATGGCAGGTTATTTTATGATCTTTTACACTTTTGTTTCTACACAACTACAATATGGAATGAGGATTTAAGTTTTTTTTTTTCCACAAAGAGAACATACATTTCTTTTCTCTTGAGTTTTTTTCCCTCCGAAAATAGGGAATTTGTTGCTTGAGAATTGGAGGTTGGCAGGGCTATCAACAGAAGGTAAACCTATCTATCTATACTGGTAATGGCATATGGCATTTCATATTTTGTTGTTCTGATGGATATTTTCTTCTATTCTGGAGTTCACCATCCGTGATATTAACCTGCTGGCACTGTTTGTTGCTCCAACTTCAATGTATATGATTTTCTGGACCATGTGCTTGATTCAAGAAGAATGTGTAACCTTAAATAAAAATCTACATCGGATTTATCTTAAGTATAATGGTTATTATCTTACTTTTGATTGACTTGTTTTAGAATGTTCCTGTACCAATCACTGCAACAAATTTTATAGTAATTGTACCTTTGACTTAGAGTCATTCAGATCAGATGTCACAATTTGGTTGTTCCTCTTGAGTAATTCAGTTAGAAGAATATATCTGGTAAAACTTAATGATAGTGACGTGGTGCAAGTTCCATAAACAGCATCTTGATCAATCTGCTTGAAATCTTTTTTCTGAAATGAACTCTTGTATAGAATATTGCCAGCTGCTGGGTAGAGATACAATCAATTTCCCTTGCTGATTTCTTTATCTTTGATGAAAGTTCTTGAAGTAAAAACAAAATCATTAAACTAGGTCCATGGTAACCCTTTCGTTTTTGGTTTTTTGTTTTTAAAATTAACCTACAAATAGTAATCCCACCCATGTGTTTATTTTTTTTGTTATCTACTTTTTAAAAACTTTTGCAAAATCTAAGCTACTTTTTTGAGGAAAAAAAAGTTAACTTTTTTCCCCTTGAAATTTAGCTAAAAATTCATATGTATTTTCAAGGAATATGGGAAACATACTAAGAAATGGTAAGAAATCAAACAATTTTTAAAAACAAAATAAAAAATAAAATGTTTATCAAACGAGACCTTAATTAAACTTAGGTGCTGTTATATTCAATGTTCAGTGGACTTCATATTTTGAATAGGCATTCGGAAGCACCAAGATGTTAGCACTGCTGTTGTGTGTGGCATAGTTGATGTCAATGGAGAATTGGCAGCTGCGGTGGCAAGTGTGGAAGCTATTGTAAGTGGATCTTTATAAATAAATTTATATTATTCCGGGTATTTAGTATTGTGATTCTTCTATATGATTAAAGAAGTACCACGAGGAACCTGAAACTAGCCTTGCACAGGAAAAATTTCTCACACCTGATTGGATTGACCGATTCAAGTGCAATATTCGTGCTGCTCCAATGCTGATGGTTGATGCAAATCTCAATCCTCTTGCTCTAGAAGTTTCTTGCCGAAGTACTACTTTTTCATAGTAGCTGCAGCTTCTTCTACTTTCTCTGTCGTTCCTTGTTAATATGATCTCTGTATATTTTTTTCCTGCGTTTTTTCTTAGTTTCTGCAGAAAATAATACTCCAGTGTGGTTTGAGCCTGTGTCAGTTGCAAAATCAAGAAGGATTGCTTCGGTTGTCAAGTATGTGAGTTCACATCTCCTCCATGCTGGTCTCTTCTCTTCAATTTTTTTTGCGTTTTCTTACTTGTGAGCAAATATTTGTTCCTCCTTTCTTTCCTTTCTGAGGTGTTTCTATTTCCATTTTTGTAAGTTATGGAAATGTTAATGGAACAATTCTTAGGTGTTTTTGAATTGGAATTGCAACCAGACTCTGACTTGCCTGCTCATGTTTGGAGATCTATTGAACATAGTTCAGTTCGTATAACAGTCGATAAAAAATGGGAAAAGATAGAATAAAAAGTTCAAAAATCAGGTACATGTAGATTGATCAGGATTACAGCTTTTATATGTGTTTCCTTTTCCCTTATTATTATGATGGAAAAAAAAACTGAAAATAGGATCTGCTGGTAAATATCATATATACGTTGGGTCAATAGTTCCTTTGTGTTGGTGGATTGATAGGTGAAGGTCTATCTGCCCTTCCTCTGTATCTGGGCTACAACTTCACATCCTATTTGGGTGATGTTTGAGTTTTCTATGCATCTTGCTACAAAATTTACATGTCATGTTGTGTTTTCGTGATATAGAGAATTAGCACTTGATCTCATTCTTACGTTTCATATTCAAAAAGTTCTAATTGACAACCATCTGATTCTCTGCTCTCTTTAATCTTATATAGCTCTTGTCATTTTTGTAGATAACTTTTGCTTCACCTAATGAAGATGAACTTATCGCCATGGCTAATGCATTATCTGGTCGAGATTTGTTTTCTCCTATCAAACGGGATAGTAGCATTACTACATGCTCAATCGAATTGCTTTTCCAACTGCTGAAATCCGCTGTATGGGTTTTGCTCGAGAAAGGCATCAAGATAGTCATTCTGACCGTTGGTTCTCATGGGGTGTTCGTATGTTTTAAAGGAGGACCAAGCTTCATGAAATTTTCTTCAGAAGGAATCAAAAAGAATAGATCCTGCAGCCAACTGTTTAGAACTATAAATTCAAGTTGTCCACCAAATACGTTTTCTGTTTCTCCTGAAAAAGAAAAAAGCTCTGCTCTTTTTGCAATACATTTCCCTGCCCTTTCTGCATCTGTAGTGAGGCTCACAGGCGCCGGAGATTGCTTGGTTGGAGGCATGCTTGCTTCTATTTGTGCAGGCTTAGATATCTTCCAAAGTACAGCCATTGGTATTGCAGCTTCCAAGGCTGCTGTCGAGACGGAAAATAATGTACCTCATGCACTTCATTCGGCTAAAATTGCAGGTACTAGTGTCACTTCTTATTGATGATTTTATTTTAGTTATTCAGTTCGGTTTAGTTTAGTTTAAGATACTTTGATAACGATTATTTACTAACCATTTCTAAATATGTTATAGCGTTATCAAATGGATCTTTAGCTCTAAATTCGTTGACAATATTTTACTTGATGACGTAAAGAACTTATCTGAGAGTTCAAAAATTTTTGCACTCTAAACAAAAATGAAAATAGATTGAATCTCCTGGACAAGAATCTTAGAATAAATAGAAAGACCTTTAAACTAAGAAAAGAAAGAGCACGATGGAAATTTGGGAATTGTGGATTGGAAGAAACATGTACATCAAACTAGAAGATTTCATGTTTTGCTTTTAGGAATGGATTGAAGTGTTTACTTTGTCTGAGTTTTTATTTTTTTATTTTTAATACTACTCCATGACCCGAAGCTTTGATTTATTTTGAGAAGTTATTAATGTCTTAACCAATTAACGGATTGAGCTCTGTTCACGAGGGTAGTATATTCGGTAATATAACATTGTTTTCTGTTTTCCAATTAATTTTAATATTCTCTCCCCACCATTTCTTTTGCAGATGATGCTAAGTCAGTGTACACAGCAGGCAGAATTGTGTTCCATGAACCCATGTACATGTCACATTTGGAATGAGCAAAATTGAAGTTGGAAAATCAGAGTCATACGATTTATGTTAAAACATGAAATTGCAAGTGTTTCTCTTCAATTGAGAATTGTGGCTGAAATTGTTTTTCCAATGAGTATGAATAATTTGATTAGAATGAGTTCAGGATCTTTTTTGAAAAAAGAAAAA

mRNA sequence

GTTTATTTTAATTCTCAAAAAGGAAAAGATGCCTTATTTATTTGGGTGTGAAATATTCAACAAGATCGACAAAATGAAAATGGAATCGATCGTCGTTGTCAACCCTGCAGGAAGAACGTAATTCGACGCTGGTCTACGGGTTTCACAGTTACAGACTTTCATAGCTCCACTGAAAACCTTGGAGATTGGTAACAATGGAGAGCAGTGCTCAGAGGCGTATAAATTTGATTCGCCGGCACCTTCTTCCGCCGCCATGTGAGTTCAACGACGCTCTTAATCTGGCGTTGACAAATGGTGGGAGCGAATTGCAAATTGGGGAATCAGAGCCTGTGGTTATTGGAGGTATGGTGCTGGACATTCATGCCATACCCTCCATCGCCGCCGTTCCGAGGTCAACTACTCCCGGCAAGATCAACTATGTATTAGGAGGCGTAGCACGGAATGTTGCTGAATGCATGTCAAAGTTCGGGACCAAACTTTTCCTGATTAGTGTTGTGGGACATGACATGGCAGGGAATTTGTTGCTTGAGAATTGGAGGTTGGCAGGGCTATCAACAGAAGGCATTCGGAAGCACCAAGATGTTAGCACTGCTGTTGTGTGTGGCATAGTTGATGTCAATGGAGAATTGGCAGCTGCGGTGGCAAGTGTGGAAGCTATTGAAAAATTTCTCACACCTGATTGGATTGACCGATTCAAGTGCAATATTCGTGCTGCTCCAATGCTGATGGTTGATGCAAATCTCAATCCTCTTGCTCTAGAAGTTTCTTGCCGAATTTCTGCAGAAAATAATACTCCAGTGTGGTTTGAGCCTGTGTCAGTTGCAAAATCAAGAAGGATTGCTTCGGTTGTCAAGTATATAACTTTTGCTTCACCTAATGAAGATGAACTTATCGCCATGGCTAATGCATTATCTGGTCGAGATTTGTTTTCTCCTATCAAACGGGATAGTAGCATTACTACATGCTCAATCGAATTGCTTTTCCAACTGCTGAAATCCGCTGTATGGGTTTTGCTCGAGAAAGGCATCAAGATAGTCATTCTGACCGTTGGTTCTCATGGGGTGTTCGTATGTTTTAAAGGAGGACCAAGCTTCATGAAATTTTCTTCAGAAGGAATCAAAAAGAATAGATCCTGCAGCCAACTGTTTAGAACTATAAATTCAAGTTGTCCACCAAATACGTTTTCTGTTTCTCCTGAAAAAGAAAAAAGCTCTGCTCTTTTTGCAATACATTTCCCTGCCCTTTCTGCATCTGTAGTGAGGCTCACAGGCGCCGGAGATTGCTTGGTTGGAGGCATGCTTGCTTCTATTTGTGCAGGCTTAGATATCTTCCAAAGTACAGCCATTGGTATTGCAGCTTCCAAGGCTGCTGTCGAGACGGAAAATAATGTACCTCATGCACTTCATTCGGCTAAAATTGCAGATGATGCTAAGTCAGTGTACACAGCAGGCAGAATTGTGTTCCATGAACCCATGTACATGTCACATTTGGAATGAGCAAAATTGAAGTTGGAAAATCAGAGTCATACGATTTATGTTAAAACATGAAATTGCAAGTGTTTCTCTTCAATTGAGAATTGTGGCTGAAATTGTTTTTCCAATGAGTATGAATAATTTGATTAGAATGAGTTCAGGATCTTTTTTGAAAAAAGAAAAA

Coding sequence (CDS)

ATGGAGAGCAGTGCTCAGAGGCGTATAAATTTGATTCGCCGGCACCTTCTTCCGCCGCCATGTGAGTTCAACGACGCTCTTAATCTGGCGTTGACAAATGGTGGGAGCGAATTGCAAATTGGGGAATCAGAGCCTGTGGTTATTGGAGGTATGGTGCTGGACATTCATGCCATACCCTCCATCGCCGCCGTTCCGAGGTCAACTACTCCCGGCAAGATCAACTATGTATTAGGAGGCGTAGCACGGAATGTTGCTGAATGCATGTCAAAGTTCGGGACCAAACTTTTCCTGATTAGTGTTGTGGGACATGACATGGCAGGGAATTTGTTGCTTGAGAATTGGAGGTTGGCAGGGCTATCAACAGAAGGCATTCGGAAGCACCAAGATGTTAGCACTGCTGTTGTGTGTGGCATAGTTGATGTCAATGGAGAATTGGCAGCTGCGGTGGCAAGTGTGGAAGCTATTGAAAAATTTCTCACACCTGATTGGATTGACCGATTCAAGTGCAATATTCGTGCTGCTCCAATGCTGATGGTTGATGCAAATCTCAATCCTCTTGCTCTAGAAGTTTCTTGCCGAATTTCTGCAGAAAATAATACTCCAGTGTGGTTTGAGCCTGTGTCAGTTGCAAAATCAAGAAGGATTGCTTCGGTTGTCAAGTATATAACTTTTGCTTCACCTAATGAAGATGAACTTATCGCCATGGCTAATGCATTATCTGGTCGAGATTTGTTTTCTCCTATCAAACGGGATAGTAGCATTACTACATGCTCAATCGAATTGCTTTTCCAACTGCTGAAATCCGCTGTATGGGTTTTGCTCGAGAAAGGCATCAAGATAGTCATTCTGACCGTTGGTTCTCATGGGGTGTTCGTATGTTTTAAAGGAGGACCAAGCTTCATGAAATTTTCTTCAGAAGGAATCAAAAAGAATAGATCCTGCAGCCAACTGTTTAGAACTATAAATTCAAGTTGTCCACCAAATACGTTTTCTGTTTCTCCTGAAAAAGAAAAAAGCTCTGCTCTTTTTGCAATACATTTCCCTGCCCTTTCTGCATCTGTAGTGAGGCTCACAGGCGCCGGAGATTGCTTGGTTGGAGGCATGCTTGCTTCTATTTGTGCAGGCTTAGATATCTTCCAAAGTACAGCCATTGGTATTGCAGCTTCCAAGGCTGCTGTCGAGACGGAAAATAATGTACCTCATGCACTTCATTCGGCTAAAATTGCAGATGATGCTAAGTCAGTGTACACAGCAGGCAGAATTGTGTTCCATGAACCCATGTACATGTCACATTTGGAATGA

Protein sequence

MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPSIAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLSTEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVDANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALSGRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSFMKFSSEGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTGAGDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAGRIVFHEPMYMSHLE

Homology

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BLAST of MC04g0488 vs. ExPASy Swiss-Prot
Match: P33020 (Uncharacterized sugar kinase YeiI OS=Escherichia coli (strain K12) OX=83333 GN=yeiI PE=3 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 4.2e-16
Identity = 71/259 (27.41%), Postives = 121/259 (46.72%), Query Frame = 0


Query: 46  VVIGGMVLDIHAIPSIAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDM 105
           VV+G + +DI  +  I     ++ PG I+   GGV RN+A  ++  G  + L+SV+G D 
Sbjct: 61  VVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHNLALLGRDVHLLSVIGDDF 120

Query: 106 AGNLLLENWRLAGLSTEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWID 165
            G +LLE  R AG++  G  +    ST+    I + + +   A+     +E+ LTP  ++
Sbjct: 121 YGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQTVLAINDTHLLEQ-LTPQLLN 180

Query: 166 RFKCNIRAAPMLMVDANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFA 225
             +  +R A +++ D NL   ALE    ++ E   PV+ + VS  K+ +I   + +I   
Sbjct: 181 GSRDLLRHAGVVLADCNLTAEALEWVFTLADE--IPVFVDTVSEFKAGKIKHWLAHIHTL 240

Query: 226 SPNEDELIAMANALSGRDLFSPIKRDSSITTC---SIELLFQLLKSAVWVLLEKGIKIVI 285
            P   EL      L G+ + S   R++++       ++ LF  L        EK  +  +
Sbjct: 241 KPTLPEL----EILWGQAITSDADRNTAVNALHQQGVQQLFVYLPDESVYCSEKDGEQFL 300

Query: 286 LTVGSHGVFVCFKGGPSFM 302
           LT  +H     F     FM
Sbjct: 301 LTAPAHTTVDSFGADDGFM 312

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BLAST of MC04g0488 vs. ExPASy Swiss-Prot
Match: P30235 (Pseudouridine kinase OS=Escherichia coli (strain K12) OX=83333 GN=psuK PE=1 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 3.4e-13
Identity = 54/199 (27.14%), Postives = 97/199 (48.74%), Query Frame = 0


Query: 46  VVIGGMVLDIHAIPSIAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDM 105
           V+IG   +D+      +     + PGKI +  GGV RN+A+ ++  G K +L+S VG D 
Sbjct: 8   VIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLSAVGSDF 67

Query: 106 AGNLLLENWRLAGLSTEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWID 165
            G  LL     +G+  +        +T+    ++D  GE+  A+  +  I   +T +++ 
Sbjct: 68  YGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMN-ISNAITAEYLA 127

Query: 166 RFKCNIRAAPMLMVDANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFA 225
           +    I+ A +++ D N++  AL  +  +    N PV+ +PVS  K  ++   +  I   
Sbjct: 128 QHGEFIQRAKVIVADCNISEEAL--AWILDNAANVPVFVDPVSAWKCVKVRDRLNQIHTL 187

Query: 226 SPNEDELIAMAN-ALSGRD 244
            PN  E   ++  ALSGR+
Sbjct: 188 KPNRLEAETLSGIALSGRE 203

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BLAST of MC04g0488 vs. ExPASy Swiss-Prot
Match: Q8R1Q9 (Ribokinase OS=Mus musculus OX=10090 GN=Rbks PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.4e-06
Identity = 58/265 (21.89%), Postives = 107/265 (40.38%), Query Frame = 0


Query: 46  VVIGGMVLDIHAIPSIAAVPRSTTPGKINYV-LGGVARNVAECMSKFGTKLFLISVVGHD 105
           VV+G  + D+ ++ S       T  G   ++  GG   N     ++ G K  ++  VG+D
Sbjct: 20  VVVGSCMTDLVSLTSRLPKTGETIHGHEFFIGFGGKGANQCVQAARLGAKAAIVCKVGND 79

Query: 106 MAGNLLLENWRLAGLSTEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWI 165
             GN  +EN +   +STE   + +D +T      + VN E    +  V     FL  + +
Sbjct: 80  SFGNDYIENLKQNHISTEFTYQTRDAATGTAS--IIVNNEGQNIIVIVAGANLFLNSEDL 139

Query: 166 DRFKCNIRAAPMLMVDANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITF 225
            +    I  A +++    ++P A   +  ++  +     F P + A +          + 
Sbjct: 140 KKAASVISRAKVMICQLEISPAASLEALTMARRSGVKTLFNP-APAMADLDPQFYTLSSI 199

Query: 226 ASPNEDELIAMANALSGRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILT 285
              NE E    A  L+G  +  P       TT            A  +LLE+G ++V++T
Sbjct: 200 FCCNESE----AEILTGHAVSDP-------TTAG---------KAAMILLERGCQVVVIT 259

Query: 286 VGSHGVFVCFKGGPSFMKFSSEGIK 310
           +G+ G  +  +  P      +E +K
Sbjct: 260 LGASGCVILSQAEPVPKHIPTEAVK 261

[Close]

BLAST of MC04g0488 vs. ExPASy Swiss-Prot
Match: P44331 (Ribokinase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=rbsK PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.3e-06
Identity = 61/249 (24.50%), Postives = 103/249 (41.37%), Query Frame = 0


Query: 61  IAAVPRSTTPGKI----NYVL--GGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENW 120
           + +VP  T PG+     NY +  GG   N A   ++ G K+  IS +G D  G  +   +
Sbjct: 16  VISVPYFTKPGETLTGQNYQIAYGGKGANQAVAAARLGAKVAFISCIGSDSIGKTMKNAF 75

Query: 121 RLAGLSTEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAA 180
              G+ T  I       T +    V  + E +  +AS       L+   + + +  I  +
Sbjct: 76  AQEGIDTTHINTVSQEMTGMAFIQVAKSSENSIVLAS--GANSHLSEMVVRQSEAQIAQS 135

Query: 181 PMLMVDANLNPLALEVSCRISAENNTPVWFEPV-SVAKSRRIASVVKYITFASPNEDELI 240
             L++        +E++ +I+ +N   V   P  +   S  + S++  IT   PNE E  
Sbjct: 136 DCLLMQLETPLSGVELAAQIAKKNGVKVVLNPAPAQILSDELLSLIDIIT---PNETE-- 195

Query: 241 AMANALSGRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVC 300
             A  L+G ++                   Q    A  V  +KGI+ V++T+G+ GVFV 
Sbjct: 196 --AEILTGVEVADE----------------QSAVKAASVFHDKGIETVMITLGAKGVFVS 239

Query: 301 FKGGPSFMK 303
            KG    +K
Sbjct: 256 RKGKSRIIK 239

[Close]

BLAST of MC04g0488 vs. ExPASy Swiss-Prot
Match: P0A9J7 (Ribokinase OS=Escherichia coli O157:H7 OX=83334 GN=rbsK PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 9.8e-05
Identity = 60/268 (22.39%), Postives = 109/268 (40.67%), Query Frame = 0


Query: 31  LTNGGSELQIGESEPVVIGGMVLDIHAIPSIAAVPRSTTPGK-INYVLGGVARNVAECMS 90
           + N GS + +G     +    +L++ + P+    P  T  G       GG   N A    
Sbjct: 1   MQNAGSLVVLGS----INADHILNLQSFPT----PGETVTGNHYQVAFGGKGANQAVAAG 60

Query: 91  KFGTKLFLISVVGHDMAGNLLLENWRLAGLSTEGIRKHQDVSTAVVCGIVDVNGELAAAV 150
           + G  +  I+  G D  G  + +      +    +   +  ST V   ++ VNGE    +
Sbjct: 61  RSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGV--ALIFVNGEGENVI 120

Query: 151 ASVEAIEKFLTPDWIDRFKCNIRAAPMLMVDANLNPLALEVSCRISAENNTPVWFEPVSV 210
                    L+P  ++  +  I  A  L++       ++  + +I+ +N T V   P   
Sbjct: 121 GIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNP--- 180

Query: 211 AKSRRIASVVKYITFASPNEDELIAMANALSGRDLFSPIKRDS-SITTCSIELLFQLLKS 270
           A +R +              DEL+A+       D+ +P + ++  +T   +E      K+
Sbjct: 181 APAREL-------------PDELLALV------DIITPNETEAEKLTGIRVENDEDAAKA 235

Query: 271 AVWVLLEKGIKIVILTVGSHGVFVCFKG 297
           A  VL EKGI+ V++T+GS GV+    G
Sbjct: 241 A-QVLHEKGIRTVLITLGSRGVWASVNG 235

[Close]

BLAST of MC04g0488 vs. NCBI nr
Match: XP_022136240.1 (uncharacterized protein LOC111007986 [Momordica charantia])

HSP 1 Score: 843 bits (2178), Expect = 4.57e-308
Identity = 433/433 (100.00%), Postives = 433/433 (100.00%), Query Frame = 0


Query: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60
           MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS
Sbjct: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60

Query: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120
           IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS
Sbjct: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120

Query: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180
           TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD
Sbjct: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180

Query: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240
           ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS
Sbjct: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240

Query: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300
           GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF
Sbjct: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300

Query: 301 MKFSSEGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTGA 360
           MKFSSEGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTGA
Sbjct: 301 MKFSSEGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTGA 360

Query: 361 GDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAGR 420
           GDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAGR
Sbjct: 361 GDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAGR 420

Query: 421 IVFHEPMYMSHLE 433
           IVFHEPMYMSHLE
Sbjct: 421 IVFHEPMYMSHLE 433

[Close]

BLAST of MC04g0488 vs. NCBI nr
Match: KAG6572020.1 (hypothetical protein SDJN03_28748, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 679 bits (1753), Expect = 2.02e-243
Identity = 345/427 (80.80%), Postives = 387/427 (90.63%), Query Frame = 0


Query: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60
           MESSA+RRINLI RHLL PPCEFN+ALN A TN G++L+IG +EPV++GGMVLDIHAIPS
Sbjct: 1   MESSARRRINLIHRHLLSPPCEFNNALNPAPTNSGNQLEIGVAEPVIVGGMVLDIHAIPS 60

Query: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120
           I+AVPRSTTPGKINY+LGGVARNVAECMSK G+  F+ISVVGHDMAGNLL ENWRLAGLS
Sbjct: 61  ISAVPRSTTPGKINYILGGVARNVAECMSKLGSNPFMISVVGHDMAGNLLFENWRLAGLS 120

Query: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180
           TEGIRKHQD+STAVVC +VDV+GELAAAVASVEAIEKFLTPDWI++F+CNIRAAP++MVD
Sbjct: 121 TEGIRKHQDISTAVVCAVVDVHGELAAAVASVEAIEKFLTPDWIEKFRCNIRAAPVVMVD 180

Query: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240
           ANLNP AL+VSC+I+AE +TPVWFEPVSVAKSRR+ASVVKYITF SPNEDELIAMANALS
Sbjct: 181 ANLNPHALKVSCQIAAEYDTPVWFEPVSVAKSRRVASVVKYITFTSPNEDELIAMANALS 240

Query: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300
           G+DLFSPIKRD++IT+ SIE  F+ LKSAVWVLLEKGIK+VILTVGSHGVFVC KGGPSF
Sbjct: 241 GQDLFSPIKRDNTITS-SIESFFEQLKSAVWVLLEKGIKVVILTVGSHGVFVCSKGGPSF 300

Query: 301 MKFSSEGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTGA 360
           +K  S+ I    S SQLFRT+ +SCPPN FSVSPE EK+S LFA+HFPAL ASVVRLTG 
Sbjct: 301 VKKISKEINNYGSSSQLFRTLATSCPPNMFSVSPETEKNSVLFAMHFPALPASVVRLTGC 360

Query: 361 GDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAGR 420
           GDCLVGGMLAS CAGL+I+QSTAIGIAA+KAAVETENNVP   HS +IADDA+ VYTAGR
Sbjct: 361 GDCLVGGMLASFCAGLNIYQSTAIGIAAAKAAVETENNVPREFHSNEIADDARLVYTAGR 420

Query: 421 IVFHEPM 427
           IVFH PM
Sbjct: 421 IVFHGPM 426

[Close]

BLAST of MC04g0488 vs. NCBI nr
Match: XP_016900345.1 (PREDICTED: pseudouridine kinase [Cucumis melo] >XP_016900346.1 PREDICTED: pseudouridine kinase [Cucumis melo])

HSP 1 Score: 668 bits (1724), Expect = 5.05e-239
Identity = 342/428 (79.91%), Postives = 380/428 (88.79%), Query Frame = 0


Query: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60
           ME+SA RRINLI RHL  PPC+F +ALNLA TNGG++LQ G +EPV++G MVLDIHAIPS
Sbjct: 1   MENSAHRRINLIHRHLHSPPCDFINALNLAQTNGGNQLQTGVAEPVIVGSMVLDIHAIPS 60

Query: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120
            +AVPRSTTPGKINY+LGGVARNVAECMSK G   FLISVVGHDMAGNLL ENWRLAGLS
Sbjct: 61  FSAVPRSTTPGKINYILGGVARNVAECMSKLGANPFLISVVGHDMAGNLLFENWRLAGLS 120

Query: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180
           TEGIRKH+D+STAVVC +VD +GELAAAVASVEAIEKFLTP+WI++FKCNIR AP+LMVD
Sbjct: 121 TEGIRKHKDISTAVVCAVVDFHGELAAAVASVEAIEKFLTPEWIEQFKCNIRDAPVLMVD 180

Query: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240
           ANLN LALEVSC+++AE NTPVWFEPVSVAKSRRIASVVKYI+F SPNEDELIAMANALS
Sbjct: 181 ANLNSLALEVSCQMAAEYNTPVWFEPVSVAKSRRIASVVKYISFTSPNEDELIAMANALS 240

Query: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300
           G+DLFSPIK+D+SIT   IE  F+ LKSAVWVLLEKGIK+V+LTVG+ GVFVC KG PSF
Sbjct: 241 GQDLFSPIKQDNSIT---IESFFEQLKSAVWVLLEKGIKVVVLTVGARGVFVCSKGQPSF 300

Query: 301 MKFSS-EGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTG 360
           MK SS E I K +SCSQLFRT+ +SCPPN F VSP  EKSS LFA+HFPAL ASVVRLTG
Sbjct: 301 MKISSKEEINKYKSCSQLFRTLATSCPPNMFPVSPVTEKSSFLFAMHFPALPASVVRLTG 360

Query: 361 AGDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAG 420
            GDCLVGGMLASICAGL+I+QSTAIGIA +KA VETENNVPH  HSAKIADDA+ VYTAG
Sbjct: 361 CGDCLVGGMLASICAGLNIYQSTAIGIATAKATVETENNVPHEFHSAKIADDARLVYTAG 420

Query: 421 RIVFHEPM 427
           RIVFH+PM
Sbjct: 421 RIVFHDPM 425

[Close]

BLAST of MC04g0488 vs. NCBI nr
Match: KAA0036523.1 (pseudouridine kinase [Cucumis melo var. makuwa])

HSP 1 Score: 667 bits (1722), Expect = 1.02e-238
Identity = 342/428 (79.91%), Postives = 380/428 (88.79%), Query Frame = 0


Query: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60
           ME+SA RRINLI RHL  PPC+F +ALNLA TNGG++LQ G +EPV++GGMVLDIHAIPS
Sbjct: 1   MENSAHRRINLIHRHLHSPPCDFINALNLAQTNGGNQLQTGVAEPVIVGGMVLDIHAIPS 60

Query: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120
            +AVPRSTTPGKINY+LGGVARNVAECMSK G   FLISVVGHDMAGNLL ENWRLAGLS
Sbjct: 61  FSAVPRSTTPGKINYILGGVARNVAECMSKLGANPFLISVVGHDMAGNLLFENWRLAGLS 120

Query: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180
           TEGIRKH+D+STAVVC +VD +GELAAAVASVEAIEKFLTP+WI++FKCNIR AP+LMVD
Sbjct: 121 TEGIRKHKDISTAVVCAVVDFHGELAAAVASVEAIEKFLTPEWIEQFKCNIRDAPVLMVD 180

Query: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240
           ANLN LALEVSC+++AE NTPVWFEPVSVAKSRRIASVVKYI+F SPNEDELIAMANALS
Sbjct: 181 ANLNSLALEVSCQMAAEYNTPVWFEPVSVAKSRRIASVVKYISFTSPNEDELIAMANALS 240

Query: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300
           G+DLFSPIK+D+SIT   IE  F+ LKSAVWVLLEKGIK+V+LTVG+ GVFVC KG PSF
Sbjct: 241 GQDLFSPIKQDNSIT---IESFFEQLKSAVWVLLEKGIKVVVLTVGARGVFVCSKGQPSF 300

Query: 301 MKFSS-EGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTG 360
           MK SS E I K +SCSQLFRT+ +SCPPN F VSP  EKSS LFA+HFPAL ASVVRLTG
Sbjct: 301 MKISSKEEINKYKSCSQLFRTLATSCPPNMFPVSPVTEKSSFLFAMHFPALPASVVRLTG 360

Query: 361 AGDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAG 420
            GDCLVGGMLASICAGL+I+QSTAIGIA +KA VETENNVPH  HSAKIADDA+ VYTAG
Sbjct: 361 CGDCLVGGMLASICAGLNIYQSTAIGIATAKATVETENNVPHEFHSAKIADDARLVYTAG 420

Query: 421 RIVFHEPM 427
           RIVFH+ M
Sbjct: 421 RIVFHDAM 425

[Close]

BLAST of MC04g0488 vs. NCBI nr
Match: KGN44322.1 (hypothetical protein Csa_016762 [Cucumis sativus])

HSP 1 Score: 652 bits (1682), Expect = 1.25e-232
Identity = 334/428 (78.04%), Postives = 375/428 (87.62%), Query Frame = 0


Query: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60
           ME+SAQRRINLI RH   PP +F + LNLA TNGG++LQ G ++PV++GGMVLDIHAIPS
Sbjct: 1   MENSAQRRINLIHRHFHTPPSQFINTLNLAQTNGGNQLQTGVAQPVIVGGMVLDIHAIPS 60

Query: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120
           I+AVPRSTTPGKINY+LGGVARNVAECMSK G   FLISVVGHDMAGNLL ENWRLAGLS
Sbjct: 61  ISAVPRSTTPGKINYILGGVARNVAECMSKLGANPFLISVVGHDMAGNLLFENWRLAGLS 120

Query: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180
           T+GIRKH+D+ TAVVC +VD +GELAAAVASVEAIEKFLTP+WI++FKCNIR AP+LMVD
Sbjct: 121 TQGIRKHKDIGTAVVCAVVDFHGELAAAVASVEAIEKFLTPEWIEQFKCNIRDAPVLMVD 180

Query: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240
           ANLN LALEVSC+++AE NTPVWFEPVSVAKSRRIASVVKYI+F SPNEDELIAMANALS
Sbjct: 181 ANLNALALEVSCQMAAEYNTPVWFEPVSVAKSRRIASVVKYISFTSPNEDELIAMANALS 240

Query: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300
           G+DLFSPIK+D+SIT   IE  F+ LKSAVWVLLEKGIK+V+LTVGS GVFVC KG PSF
Sbjct: 241 GQDLFSPIKQDNSIT---IESFFEELKSAVWVLLEKGIKVVVLTVGSRGVFVCSKGQPSF 300

Query: 301 MKFSSEG-IKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTG 360
            K SSE  + K +S SQLFRT+ +SCPPN F VSP  EKSS LFAIHFPAL ASVVRLTG
Sbjct: 301 TKISSEEELNKYKSSSQLFRTLATSCPPNMFPVSPVTEKSSVLFAIHFPALPASVVRLTG 360

Query: 361 AGDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAG 420
            GDCLVGGML SICAGL+I+QSTAIGIA +KA VETE+NVPH  H+AKIADDA+ VYTAG
Sbjct: 361 CGDCLVGGMLTSICAGLNIYQSTAIGIATAKATVETEHNVPHEFHAAKIADDARLVYTAG 420

Query: 421 RIVFHEPM 427
           R+VFH PM
Sbjct: 421 RLVFHGPM 425

[Close]

BLAST of MC04g0488 vs. ExPASy TrEMBL
Match: A0A6J1C3C2 (uncharacterized protein LOC111007986 OS=Momordica charantia OX=3673 GN=LOC111007986 PE=4 SV=1)

HSP 1 Score: 843 bits (2178), Expect = 2.21e-308
Identity = 433/433 (100.00%), Postives = 433/433 (100.00%), Query Frame = 0


Query: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60
           MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS
Sbjct: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60

Query: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120
           IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS
Sbjct: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120

Query: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180
           TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD
Sbjct: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180

Query: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240
           ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS
Sbjct: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240

Query: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300
           GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF
Sbjct: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300

Query: 301 MKFSSEGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTGA 360
           MKFSSEGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTGA
Sbjct: 301 MKFSSEGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTGA 360

Query: 361 GDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAGR 420
           GDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAGR
Sbjct: 361 GDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAGR 420

Query: 421 IVFHEPMYMSHLE 433
           IVFHEPMYMSHLE
Sbjct: 421 IVFHEPMYMSHLE 433

[Close]

BLAST of MC04g0488 vs. ExPASy TrEMBL
Match: A0A1S4DWH5 (pseudouridine kinase OS=Cucumis melo OX=3656 GN=LOC103489607 PE=4 SV=1)

HSP 1 Score: 668 bits (1724), Expect = 2.45e-239
Identity = 342/428 (79.91%), Postives = 380/428 (88.79%), Query Frame = 0


Query: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60
           ME+SA RRINLI RHL  PPC+F +ALNLA TNGG++LQ G +EPV++G MVLDIHAIPS
Sbjct: 1   MENSAHRRINLIHRHLHSPPCDFINALNLAQTNGGNQLQTGVAEPVIVGSMVLDIHAIPS 60

Query: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120
            +AVPRSTTPGKINY+LGGVARNVAECMSK G   FLISVVGHDMAGNLL ENWRLAGLS
Sbjct: 61  FSAVPRSTTPGKINYILGGVARNVAECMSKLGANPFLISVVGHDMAGNLLFENWRLAGLS 120

Query: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180
           TEGIRKH+D+STAVVC +VD +GELAAAVASVEAIEKFLTP+WI++FKCNIR AP+LMVD
Sbjct: 121 TEGIRKHKDISTAVVCAVVDFHGELAAAVASVEAIEKFLTPEWIEQFKCNIRDAPVLMVD 180

Query: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240
           ANLN LALEVSC+++AE NTPVWFEPVSVAKSRRIASVVKYI+F SPNEDELIAMANALS
Sbjct: 181 ANLNSLALEVSCQMAAEYNTPVWFEPVSVAKSRRIASVVKYISFTSPNEDELIAMANALS 240

Query: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300
           G+DLFSPIK+D+SIT   IE  F+ LKSAVWVLLEKGIK+V+LTVG+ GVFVC KG PSF
Sbjct: 241 GQDLFSPIKQDNSIT---IESFFEQLKSAVWVLLEKGIKVVVLTVGARGVFVCSKGQPSF 300

Query: 301 MKFSS-EGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTG 360
           MK SS E I K +SCSQLFRT+ +SCPPN F VSP  EKSS LFA+HFPAL ASVVRLTG
Sbjct: 301 MKISSKEEINKYKSCSQLFRTLATSCPPNMFPVSPVTEKSSFLFAMHFPALPASVVRLTG 360

Query: 361 AGDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAG 420
            GDCLVGGMLASICAGL+I+QSTAIGIA +KA VETENNVPH  HSAKIADDA+ VYTAG
Sbjct: 361 CGDCLVGGMLASICAGLNIYQSTAIGIATAKATVETENNVPHEFHSAKIADDARLVYTAG 420

Query: 421 RIVFHEPM 427
           RIVFH+PM
Sbjct: 421 RIVFHDPM 425

[Close]

BLAST of MC04g0488 vs. ExPASy TrEMBL
Match: A0A5A7T0V8 (Pseudouridine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold191G00170 PE=4 SV=1)

HSP 1 Score: 667 bits (1722), Expect = 4.93e-239
Identity = 342/428 (79.91%), Postives = 380/428 (88.79%), Query Frame = 0


Query: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60
           ME+SA RRINLI RHL  PPC+F +ALNLA TNGG++LQ G +EPV++GGMVLDIHAIPS
Sbjct: 1   MENSAHRRINLIHRHLHSPPCDFINALNLAQTNGGNQLQTGVAEPVIVGGMVLDIHAIPS 60

Query: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120
            +AVPRSTTPGKINY+LGGVARNVAECMSK G   FLISVVGHDMAGNLL ENWRLAGLS
Sbjct: 61  FSAVPRSTTPGKINYILGGVARNVAECMSKLGANPFLISVVGHDMAGNLLFENWRLAGLS 120

Query: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180
           TEGIRKH+D+STAVVC +VD +GELAAAVASVEAIEKFLTP+WI++FKCNIR AP+LMVD
Sbjct: 121 TEGIRKHKDISTAVVCAVVDFHGELAAAVASVEAIEKFLTPEWIEQFKCNIRDAPVLMVD 180

Query: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240
           ANLN LALEVSC+++AE NTPVWFEPVSVAKSRRIASVVKYI+F SPNEDELIAMANALS
Sbjct: 181 ANLNSLALEVSCQMAAEYNTPVWFEPVSVAKSRRIASVVKYISFTSPNEDELIAMANALS 240

Query: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300
           G+DLFSPIK+D+SIT   IE  F+ LKSAVWVLLEKGIK+V+LTVG+ GVFVC KG PSF
Sbjct: 241 GQDLFSPIKQDNSIT---IESFFEQLKSAVWVLLEKGIKVVVLTVGARGVFVCSKGQPSF 300

Query: 301 MKFSS-EGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTG 360
           MK SS E I K +SCSQLFRT+ +SCPPN F VSP  EKSS LFA+HFPAL ASVVRLTG
Sbjct: 301 MKISSKEEINKYKSCSQLFRTLATSCPPNMFPVSPVTEKSSFLFAMHFPALPASVVRLTG 360

Query: 361 AGDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAG 420
            GDCLVGGMLASICAGL+I+QSTAIGIA +KA VETENNVPH  HSAKIADDA+ VYTAG
Sbjct: 361 CGDCLVGGMLASICAGLNIYQSTAIGIATAKATVETENNVPHEFHSAKIADDARLVYTAG 420

Query: 421 RIVFHEPM 427
           RIVFH+ M
Sbjct: 421 RIVFHDAM 425

[Close]

BLAST of MC04g0488 vs. ExPASy TrEMBL
Match: A0A0A0K7L6 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G253770 PE=4 SV=1)

HSP 1 Score: 652 bits (1682), Expect = 6.04e-233
Identity = 334/428 (78.04%), Postives = 375/428 (87.62%), Query Frame = 0


Query: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60
           ME+SAQRRINLI RH   PP +F + LNLA TNGG++LQ G ++PV++GGMVLDIHAIPS
Sbjct: 1   MENSAQRRINLIHRHFHTPPSQFINTLNLAQTNGGNQLQTGVAQPVIVGGMVLDIHAIPS 60

Query: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120
           I+AVPRSTTPGKINY+LGGVARNVAECMSK G   FLISVVGHDMAGNLL ENWRLAGLS
Sbjct: 61  ISAVPRSTTPGKINYILGGVARNVAECMSKLGANPFLISVVGHDMAGNLLFENWRLAGLS 120

Query: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180
           T+GIRKH+D+ TAVVC +VD +GELAAAVASVEAIEKFLTP+WI++FKCNIR AP+LMVD
Sbjct: 121 TQGIRKHKDIGTAVVCAVVDFHGELAAAVASVEAIEKFLTPEWIEQFKCNIRDAPVLMVD 180

Query: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240
           ANLN LALEVSC+++AE NTPVWFEPVSVAKSRRIASVVKYI+F SPNEDELIAMANALS
Sbjct: 181 ANLNALALEVSCQMAAEYNTPVWFEPVSVAKSRRIASVVKYISFTSPNEDELIAMANALS 240

Query: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300
           G+DLFSPIK+D+SIT   IE  F+ LKSAVWVLLEKGIK+V+LTVGS GVFVC KG PSF
Sbjct: 241 GQDLFSPIKQDNSIT---IESFFEELKSAVWVLLEKGIKVVVLTVGSRGVFVCSKGQPSF 300

Query: 301 MKFSSEG-IKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTG 360
            K SSE  + K +S SQLFRT+ +SCPPN F VSP  EKSS LFAIHFPAL ASVVRLTG
Sbjct: 301 TKISSEEELNKYKSSSQLFRTLATSCPPNMFPVSPVTEKSSVLFAIHFPALPASVVRLTG 360

Query: 361 AGDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAG 420
            GDCLVGGML SICAGL+I+QSTAIGIA +KA VETE+NVPH  H+AKIADDA+ VYTAG
Sbjct: 361 CGDCLVGGMLTSICAGLNIYQSTAIGIATAKATVETEHNVPHEFHAAKIADDARLVYTAG 420

Query: 421 RIVFHEPM 427
           R+VFH PM
Sbjct: 421 RLVFHGPM 425

[Close]

BLAST of MC04g0488 vs. ExPASy TrEMBL
Match: A0A6J1I9U9 (putative pentatricopeptide repeat-containing protein At3g18840 OS=Cucurbita maxima OX=3661 GN=LOC111470970 PE=4 SV=1)

HSP 1 Score: 654 bits (1687), Expect = 4.52e-224
Identity = 335/433 (77.37%), Postives = 381/433 (87.99%), Query Frame = 0


Query: 1   MESSAQRRINLIRRHLLPPPCEFNDALNLALTNGGSELQIGESEPVVIGGMVLDIHAIPS 60
           MESSA+RRINLI RHLL PPCEFN+ALN A TN G++L+IG +EPV++GGMVLDIHAIPS
Sbjct: 1   MESSARRRINLIHRHLLSPPCEFNNALNPAPTNSGNQLEIGVAEPVIVGGMVLDIHAIPS 60

Query: 61  IAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGHDMAGNLLLENWRLAGLS 120
           I+AVPRSTTPGKINY+LGGVARNVAECMSK G+  F+ISVVGHDMAGNLL ENWRLAGLS
Sbjct: 61  ISAVPRSTTPGKINYILGGVARNVAECMSKLGSTPFMISVVGHDMAGNLLFENWRLAGLS 120

Query: 121 TEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDWIDRFKCNIRAAPMLMVD 180
           TEGIRKHQD+STAVVC +VDV+GELAAAVASVEAIEKFLTPDWI++FKCNIRAAP++MVD
Sbjct: 121 TEGIRKHQDISTAVVCAVVDVHGELAAAVASVEAIEKFLTPDWIEKFKCNIRAAPVVMVD 180

Query: 181 ANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYITFASPNEDELIAMANALS 240
           ANL+P AL+VSC+I+AE +TPVWFEPVSVAKSRR+ASVVKYI+F SPNEDELIAMANALS
Sbjct: 181 ANLSPHALKVSCQIAAEYDTPVWFEPVSVAKSRRVASVVKYISFTSPNEDELIAMANALS 240

Query: 241 GRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVILTVGSHGVFVCFKGGPSF 300
           G+DLFSPIKRD++ITTCSIE  F+ LKSAVWVLLEKG+K+VILTVGS GVFVC KG PSF
Sbjct: 241 GQDLFSPIKRDNTITTCSIESFFEQLKSAVWVLLEKGVKVVILTVGSRGVFVCSKGCPSF 300

Query: 301 MKFSSEGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALFAIHFPALSASVVRLTGA 360
           +K  S+ I    S SQLFRT+ +SCPPN FSVSPE EK+S LFA+HFPAL ASVVRLTG 
Sbjct: 301 VKKISKEINNYGSSSQLFRTLATSCPPNMFSVSPETEKNSVLFAMHFPALPASVVRLTGC 360

Query: 361 GDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHALHSAKIADDAKSVYTAGR 420
           GDCLVGGMLASICAGL+I+QSTAIGIAA+KAAVETENNVPH  HS +IA +     +   
Sbjct: 361 GDCLVGGMLASICAGLNIYQSTAIGIAAAKAAVETENNVPHEFHSNEIAVNLLPALS--- 420

Query: 421 IVFHEPMYMSHLE 433
            V+   M M HL+
Sbjct: 421 -VWQYYMQMKHLK 429

[Close]

BLAST of MC04g0488 vs. TAIR 10
Match: AT1G49350.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.3e-100
Identity = 190/385 (49.35%), Postives = 269/385 (69.87%), Query Frame = 0


Query: 44  EPVVIGGMVLDIHAIPSIAAVPRSTTPGKINYVLGGVARNVAECMSKFGTKLFLISVVGH 103
           EPV+IG ++LD+HA PS   +  +T PG++ +  GGVARNVA+C+ K G   F+I  +G 
Sbjct: 2   EPVIIGALILDVHAKPSTTPISGTTVPGQVLFAPGGVARNVADCIFKLGITPFMIGTLGL 61

Query: 104 DMAGNLLLENWRLAGLSTEGIRKHQDVSTAVVCGIVDVNGELAAAVASVEAIEKFLTPDW 163
           D   N+LL+ W+   LS +GI + +D+ST +V  + D NGE+AA VA V+A+E FLTP+W
Sbjct: 62  DGPANVLLKEWK---LSMKGILRREDISTPIVSLVYDTNGEVAAGVAGVDAVENFLTPEW 121

Query: 164 IDRFKCNIRAAPMLMVDANLNPLALEVSCRISAENNTPVWFEPVSVAKSRRIASVVKYIT 223
           I RF+ NI +A +LMVDANL+ LALE SC+++AE++ PVWFEPVSV KS+RIAS+ KY+T
Sbjct: 122 IQRFEYNISSARLLMVDANLSSLALEASCKLAAESSVPVWFEPVSVTKSQRIASIAKYVT 181

Query: 224 FASPNEDELIAMANALSGRDLFSPIKRDSSITTCSIELLFQLLKSAVWVLLEKGIKIVIL 283
             SPN+DELIAMANAL  ++LF P + D +    SIE +F+ LK A+ VLL+ G+K+VI+
Sbjct: 182 IVSPNQDELIAMANALCAKNLFHPFRSDEN--KLSIEDMFRALKPAILVLLKNGVKVVIV 241

Query: 284 TVGSHGVFVCFKGGPSFMKFSSEGIKKNRSCSQLFRTINSSCPPNTFSVSPEKEKSSALF 343
           T+GS+G  +C KG P   K +    +K     ++F+ + S C PN FS      +S +LF
Sbjct: 242 TLGSNGALLCSKGNP---KKALNIDRKFLRSGEVFKRVQSVCSPNRFS-ELGSNRSPSLF 301

Query: 344 AIHFPALSASVVRLTGAGDCLVGGMLASICAGLDIFQSTAIGIAASKAAVETENNVPHAL 403
           A+HFP + A V +LTGAGDCLVGG +AS+  GLD+ QS A+GIA++KAAVE+++NVP   
Sbjct: 302 AMHFPTIPAKVKKLTGAGDCLVGGTVASLSDGLDLIQSLAVGIASAKAAVESDDNVPPEF 361

Query: 404 HSAKIADDAKSVYTAGR-IVFHEPM 428
               I+ DA+ VY   + ++ H+ M
Sbjct: 362 KLDLISGDAELVYNGAKMLMVHQSM 377

The following BLAST results are available for this feature:

BLAST of MC04g0488 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P33020	4.2e-16	27.41	Uncharacterized sugar kinase YeiI OS=Escherichia coli (strain K12) OX=83333 GN=y...	[more]
P30235	3.4e-13	27.14	Pseudouridine kinase OS=Escherichia coli (strain K12) OX=83333 GN=psuK PE=1 SV=2	[more]
Q8R1Q9	1.4e-06	21.89	Ribokinase OS=Mus musculus OX=10090 GN=Rbks PE=1 SV=1	[more]
P44331	2.3e-06	24.50	Ribokinase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)...	[more]
P0A9J7	9.8e-05	22.39	Ribokinase OS=Escherichia coli O157:H7 OX=83334 GN=rbsK PE=3 SV=1	[more]

BLAST of MC04g0488 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136240.1	4.57e-308	100.00	uncharacterized protein LOC111007986 [Momordica charantia]	[more]
KAG6572020.1	2.02e-243	80.80	hypothetical protein SDJN03_28748, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_016900345.1	5.05e-239	79.91	PREDICTED: pseudouridine kinase [Cucumis melo] >XP_016900346.1 PREDICTED: pseudo...	[more]
KAA0036523.1	1.02e-238	79.91	pseudouridine kinase [Cucumis melo var. makuwa]	[more]
KGN44322.1	1.25e-232	78.04	hypothetical protein Csa_016762 [Cucumis sativus]	[more]

BLAST of MC04g0488 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C3C2	2.21e-308	100.00	uncharacterized protein LOC111007986 OS=Momordica charantia OX=3673 GN=LOC111007...	[more]
A0A1S4DWH5	2.45e-239	79.91	pseudouridine kinase OS=Cucumis melo OX=3656 GN=LOC103489607 PE=4 SV=1	[more]
A0A5A7T0V8	4.93e-239	79.91	Pseudouridine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold191...	[more]
A0A0A0K7L6	6.04e-233	78.04	PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G253770 PE=4 S...	[more]
A0A6J1I9U9	4.52e-224	77.37	putative pentatricopeptide repeat-containing protein At3g18840 OS=Cucurbita maxi...	[more]

BLAST of MC04g0488 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G49350.1	1.3e-100	49.35	pfkB-like carbohydrate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029056	Ribokinase-like	GENE3D	3.40.1190.20		coord: 42..420 e-value: 8.3E-60 score: 204.5
IPR029056	Ribokinase-like	SUPERFAMILY	53613	Ribokinase-like	coord: 46..400
IPR011611	Carbohydrate kinase PfkB	PFAM	PF00294	PfkB	coord: 46..300 e-value: 6.9E-19 score: 68.3
None	No IPR available	PANTHER	PTHR43085	HEXOKINASE FAMILY MEMBER	coord: 1..422
None	No IPR available	PANTHER	PTHR43085:SF26	PFKB-LIKE CARBOHYDRATE KINASE FAMILY PROTEIN	coord: 1..422
None	No IPR available	CDD	cd01941	YeiC_kinase_like	coord: 44..395 e-value: 1.00898E-64 score: 207.551
IPR002173	Carbohydrate/purine kinase, PfkB, conserved site	PROSITE	PS00583	PFKB_KINASES_1	coord: 78..102

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0488.1	MC04g0488.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0016773	phosphotransferase activity, alcohol group as acceptor