MC04g0487 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0487 vs. ExPASy Swiss-Prot
Match: Q9SXA1 (Phosphatidylinositol 4-kinase alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PI4KA1 PE=1 SV=2)

HSP 1 Score: 3023.4 bits (7837), Expect = 0.0e+00
Identity = 1529/2051 (74.55%), Postives = 1753/2051 (85.47%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            MEAL ELCD+IA+NP  FSEKL+WIC RCP +E  LA SP +SRS LNAVLAVAR++SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            P+S+  R KSVV EF+ +IP SF  SFWP S+ +  I+SFY +F++Y S A +LS EF T
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EISGFTSEVVLSAI---DDCSEGSP-ISRAFLVAMSKSFIPILPSDADKLVSCILDRFLI 180
            E++ FT EVV++AI      S+G P IS+AFLVA+S+ F  IL SD DKL++ +LD+F++
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  SEAAPGTPREQIRANS-----EASSSQGSPLSVSHHPLNGG--LSPGNENGQVSGSLSSG 240
            +  AP +P+EQ + NS     + SSSQGSP+S + +P       SPG+E       ++S 
Sbjct: 181  NR-APASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDE-------VASH 240

Query: 241  GSRITDDATSASSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESI 300
            GS +     S+ S  S +MNG S++WKSGVDQL        GG     RQQV  FEDESI
Sbjct: 241  GSNL-----SSKSSSSVVMNGGSIVWKSGVDQLSFGFSEGSGGANPVFRQQVASFEDESI 300

Query: 301  ENLEKQEIAFKLVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLK 360
            E+LEKQEIAF+L+THILD    D++L +Q+R + K+QLQ++  FLK +KRDW+EQG VLK
Sbjct: 301  ESLEKQEIAFRLITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQVLK 360

Query: 361  ARINTKLLVYQAAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRIC 420
             R+N KL VYQAAA++K+K++ SL+ DGK +K+L+ ET ALL+DAA+ACL SVWRK++ C
Sbjct: 361  TRVNAKLSVYQAAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMKAC 420

Query: 421  EELFGSLLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCC 480
            EELF SLL+G+A+IAVARGGQPLRVLLIRLKPLVL VCA  D     QGAM ES+ KT C
Sbjct: 421  EELFDSLLSGIAKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKTSC 480

Query: 481  EIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVN 540
             IIES W KDRAPVD FIMGLA+SIR+RND EEQ D+EKQ   +QLNVIRLLA + VAV 
Sbjct: 481  VIIESAWAKDRAPVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVAVK 540

Query: 541  KSEVVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLS 600
            K EV DMILPLFIESLEEGDASTP  LRLQLLDAVSR+A+LGF+KSYRET+VLMTRSYLS
Sbjct: 541  KPEVADMILPLFIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSYLS 600

Query: 601  KLSSIGSSESRTLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSA 660
            KLSS+GS ES+T APEA TERVETLPAGFL IASGL   KLRS+YRHRLLSLCSDVGL+A
Sbjct: 601  KLSSVGSVESKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGLAA 660

Query: 661  ESKSGRSGADFLGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQ 720
            ESKSG SG DFLGPLLPAVAEICSDFDPTM++EPSLLKLFRNLWFYIALFGLAPPI K+ 
Sbjct: 661  ESKSGGSGVDFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVKTP 720

Query: 721  L-QTKSVSSMLNSVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDE 780
                KS S+ +NSVGSM A ALQAV GPYMW+ QW+ AVQ IAQGTPPLVVSSVKWLEDE
Sbjct: 721  TPPLKSTSNSVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLEDE 780

Query: 781  LELNALHNPGSRQGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEI 840
            LELNALHNPGSR+G+GNEK A  QR ALS ALGGRVD+AAM+TISGVKATYLLAV+FLEI
Sbjct: 781  LELNALHNPGSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFLEI 840

Query: 841  IRFSSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDD 900
            IRF SNGG+LNG S+++ASRSAFSCVFEYLKTPNL PAVSQCLTA+VH AFETAVSWL+D
Sbjct: 841  IRFISNGGILNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWLED 900

Query: 901  RISETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLD 960
            RIS TG +A  R+ T +AH CFLI+SMSQRDEHVRDI+VNLLTQLRDKFPQV+W+SSCLD
Sbjct: 901  RISLTGKDARNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSCLD 960

Query: 961  SLLFSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNT 1020
            SLLFS+H++ PS VV DPAW   VRSLYQ VVREWII SLSYAPCTSQGLLQ+KLCK NT
Sbjct: 961  SLLFSVHDNTPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKANT 1020

Query: 1021 WQRAQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEV 1080
            WQRAQ T DV+SLLSEI+IGTGKN+ W+GI+TANIPAVM AAAAASGA+LK++EAFNLEV
Sbjct: 1021 WQRAQTTTDVVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNLEV 1080

Query: 1081 LGTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQ 1140
            LGTG+VSATVKCNHAGEIAGMRRLYNSIGGFQ+G    T  GFG G QRLISGAF Q PQ
Sbjct: 1081 LGTGVVSATVKCNHAGEIAGMRRLYNSIGGFQSG---STPSGFGGGLQRLISGAFSQAPQ 1140

Query: 1141 NEDDSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIE 1200
             EDDSFN +LI +FV+ LQQFV+ AEKG  ++K +FRETCSQATALLLSNL  ESKTN+E
Sbjct: 1141 PEDDSFNEMLIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTNVE 1200

Query: 1201 GFARLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRG 1260
            GF++L+RLLCWCPAYISTPDA+ETG+FIWTWLVSAAP+L S VLAELVDAW+WTIDTKRG
Sbjct: 1201 GFSQLLRLLCWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTKRG 1260

Query: 1261 LFASEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ 1320
            LFAS+V+YSGPAAKLRPHLSPGEPE  PE DPV+QI+AHR+WLGF IDRFEVVRHNS EQ
Sbjct: 1261 LFASDVRYSGPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSAEQ 1320

Query: 1321 VLLFGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDR 1380
            +LL GR+LQ ST   W F+RHPAA G+FF+LMLLGLKFCSCQ+QGN+Q F++GL+LLEDR
Sbjct: 1321 LLLLGRMLQRSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLEDR 1380

Query: 1381 IYRASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGIS 1440
            IYR SLGWFAH+PEWYDV   NF  SEA SVS+F+H+LS++     Q D+K + RE+G +
Sbjct: 1381 IYRTSLGWFAHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESG-N 1440

Query: 1441 VVNSKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPKLS 1500
            +++  DQYHPVWG ++NY +G+EKR+QLLLMLCQHEADRL+VWAQP + K+S  SR K+S
Sbjct: 1441 LIDVTDQYHPVWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLKIS 1500

Query: 1501 SEKWIEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTP 1560
            SEKW E+ +TAFSVDPRIA S+ SRFP NAS++ E+ QLVQ +I+D+R+IPEALPYFVTP
Sbjct: 1501 SEKWTEYAKTAFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFVTP 1560

Query: 1561 KAVDENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQ 1620
            K V+ENS LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE+VTFFMPQ
Sbjct: 1561 KNVEENSVLLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFMPQ 1620

Query: 1621 LVQALRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALL 1680
            LVQ+LRYD+GRLVEGYLLRA QRSDIFAHILIWHLQGE  V ++ KD +  KN +F  +L
Sbjct: 1621 LVQSLRYDDGRLVEGYLLRATQRSDIFAHILIWHLQGE-DVQETPKDGSIDKNAAFQEIL 1680

Query: 1681 PVVRQHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGE 1740
            P VRQHIIDGF+P ALD+F REF+FFDKVTSISGVLFPLPK+ERRAGIRRELEKIEM+G+
Sbjct: 1681 PQVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIEMQGD 1740

Query: 1741 DLYLPTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDC 1800
            DLYLPTA NKLVRGI+VDSGIPLQSAAKVPIMITFNV++RDGD +D+KPQACIFKVGDDC
Sbjct: 1741 DLYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKVGDDC 1800

Query: 1801 RQDVLALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGG 1860
            RQDVLALQVI+LLRDIFQA G+NLYLFPYGVLPTG  RGIIEVVPNTRSRSQMGETTDGG
Sbjct: 1801 RQDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGETTDGG 1860

Query: 1861 LYEIFQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHID 1920
            LYEIFQQDYGPVGS  FE ARENF+ISSAGYAVASLLLQPKDRHNGNLL D+ GRLVHID
Sbjct: 1861 LYEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHID 1920

Query: 1921 FGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMD 1980
            FGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR MD
Sbjct: 1921 FGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMD 1980

Query: 1981 GIINTVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYD 2039
            GII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FMI VCTDAYNKWTTAGYD
Sbjct: 1981 GIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYD 2028

BLAST of MC04g0487 vs. ExPASy Swiss-Prot
Match: Q9C680 (Phosphatidylinositol 4-kinase alpha 2 OS=Arabidopsis thaliana OX=3702 GN=PI4KA2 PE=3 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 2.4e-202
Identity = 351/420 (83.57%), Postives = 376/420 (89.52%), Query Frame = 0

Query: 1619 LVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVRQHIIDGF 1678
            +VEGYLLRAAQRSDIFAH LIWHLQGE SV +  KD    KN SF  +L  VRQHI+DGF
Sbjct: 115  MVEGYLLRAAQRSDIFAHTLIWHLQGE-SVEEIVKDGAFDKNASFQEILSDVRQHIVDGF 174

Query: 1679 TPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKL 1738
            TPKAL+ F REF+FF+KVTSISG L PLPK+ER AGIRRELEKI+M+GEDLYLPTA NKL
Sbjct: 175  TPKALNFFSREFDFFEKVTSISGALLPLPKEERVAGIRRELEKIKMQGEDLYLPTAPNKL 234

Query: 1739 VRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDVLALQVIA 1798
            VR IQVDSGIPLQSAAKVPIMITFNVV+ DGD ND+KPQACIFKVGDDCRQDVLALQVI+
Sbjct: 235  VRVIQVDSGIPLQSAAKVPIMITFNVVDLDGDHNDVKPQACIFKVGDDCRQDVLALQVIS 294

Query: 1799 LLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGP 1858
            LL DIFQAVG+NLYLFPYGVLPT        VVPNTRSRSQMGETTDGGLYEIFQQ+YG 
Sbjct: 295  LLGDIFQAVGLNLYLFPYGVLPT--------VVPNTRSRSQMGETTDGGLYEIFQQNYGL 354

Query: 1859 VGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFILETSPGG 1918
            VGS  FE AR NF+ISSAGYAVASLLLQPKDRHNGNLL D+ GRLVHIDFGFILETSPGG
Sbjct: 355  VGSTTFETARANFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGG 414

Query: 1919 NMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLD 1978
            NMRFE+AHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR+MD II+TV +ML+
Sbjct: 415  NMRFENAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRYMDEIISTVQMMLE 474

Query: 1979 SGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2038
            SGLPCFSRGDPIGNLRKRFHPEMSEREAA FMI VCTDAYNKWTTAGYDLIQYLQQG+EK
Sbjct: 475  SGLPCFSRGDPIGNLRKRFHPEMSEREAALFMINVCTDAYNKWTTAGYDLIQYLQQGVEK 525

HSP 2 Score: 117.9 bits (294), Expect = 1.4e-24
Identity = 56/117 (47.86%), Postives = 78/117 (66.67%), Query Frame = 0

Query: 1   MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
           ME L ELCD+I QNP  F +KL WIC +CP S++  A SP  S+S LNA+LAV R+LSK 
Sbjct: 1   MERLNELCDIIEQNPKQFLDKLPWICQQCPQSKLLRAESPRFSQSHLNAILAVTRILSKI 60

Query: 61  PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTE 118
            D+     K VV++F++++P SF+ SFWP S   ++I++FY  F+ Y S   +L  E
Sbjct: 61  VDTTDENAKFVVLDFLQTVPKSFHRSFWPYSLSLESISAFYCSFLGYVSCLSQLMVE 117

BLAST of MC04g0487 vs. ExPASy Swiss-Prot
Match: O02811 (Phosphatidylinositol 4-kinase alpha OS=Bos taurus OX=9913 GN=PI4KA PE=2 SV=2)

HSP 1 Score: 601.3 bits (1549), Expect = 4.1e-170
Identity = 477/1699 (28.08%), Postives = 760/1699 (44.73%), Query Frame = 0

Query: 456  SQGAMFESV----LKTCCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQAV 515
            SQ +M+E +    +   C  +++  T D   V+ F+     S+ +R    ++ DK+   +
Sbjct: 557  SQPSMYEQLRDIAIDNVCRCLKAGLTVDPVIVEAFL----ASLSNRLYISQESDKDAHLI 616

Query: 516  PMQLNVIRLLAKMTVAVNKS-EVVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMASL 575
            P   + IR L  + VA+  + +V++ IL +    L++     P  L + ++D +  +   
Sbjct: 617  PD--HTIRALGHIAVALRDTPKVMEPILQI----LQQKFCQPPSPLDVLIIDQLGCLVIT 676

Query: 576  GFEKSYRETIVLMTRSYLSKLSSIGSSESRTLAPEANTERVETLPAGFLLIASGLKGAKL 635
            G +  Y+E   L  +  + K SS+  S ++             +      IA+ ++   L
Sbjct: 677  GNQYIYQEVWNLFQQISV-KASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHL 736

Query: 636  RSEYRHRLLSLCSDVGLSAESKSGR-----------SGADFLGPLLPAVAEICSDFDPTM 695
              E    LL L   +GL  +  S R           S A  LG L+P +A +     P  
Sbjct: 737  VDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIK 796

Query: 696  NIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLNSVGSMGAIALQAVSGPYMW 755
              +P L KLFR+ W Y  L G                               AV G  +W
Sbjct: 797  EPKPRLQKLFRDFWLYSVLMGF------------------------------AVEGSGLW 856

Query: 756  NTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSRQGSGNEKAAVAQRAALSAA 815
              +W   V  IA  +P L   S + L   L+ N+           N+    A+ + L + 
Sbjct: 857  PEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNS--------AMKNDTVTPAELSELRST 916

Query: 816  LGGRVD-----IAAMSTISGVKATYLLAVSFLEIIRFSSNGGVLNGGSNLNASRSAFSCV 875
            +   +D      A +  +    +TYLL+V  LE +R      VL   S+  A      C 
Sbjct: 917  IVNLLDPAPEVSALIGKLDFAMSTYLLSVYRLEYMR------VLR--SSDPARFQVMFCY 976

Query: 876  FEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETGNEAEVRDSTLFAHTCFLIRS 935
            FE          + QC+ A+    F+  ++ + ++     NE E     L  H  FL+ S
Sbjct: 977  FEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEE-----LERHAQFLLVS 1036

Query: 936  MSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL-----------FSMHNDAPSAVV 995
             +   + +R +A   L+ L DKFP ++W+ + L ++L             +H D P   +
Sbjct: 1037 FNHVHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDI 1096

Query: 996  TDPAWVVTVRSLYQGVVREWIIK------------SLSYAPCTSQGLLQEKLCKVNTWQR 1055
             D  + +TV   Y+   RE I+K            ++ +AP  ++  LQE L K   W  
Sbjct: 1097 PDVPYRITVPDTYE--ARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLSKHQNWVS 1156

Query: 1056 --AQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVL 1115
              +QHT   ++  S +    G N   T +        +T   A    D            
Sbjct: 1157 GLSQHTGLAMATESVLHY-AGYNKQSTSLGATQ----LTERPACVKKD-----------Y 1216

Query: 1116 GTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQN 1175
               M S  ++  +AGE+ GM R  ++ G                                
Sbjct: 1217 SNFMASLNLRNRYAGEVYGMIRFSDATG-------------------------------- 1276

Query: 1176 EDDSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEG 1235
                 N +++ +   +L         G G D  ++ +   + TA+L+S+   +       
Sbjct: 1277 HTSDLNKMMVQELKAAL---------GAG-DAQQYTQAMFKLTAMLISSRDCD------- 1336

Query: 1236 FARLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGL 1295
              +L+  LCW P  +     +ET +  W WL++    +    + E+  AW  T++ K GL
Sbjct: 1337 -PQLLHHLCWGPLQMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWQMTVEQKFGL 1396

Query: 1296 FASEVKYSGP-AAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ 1355
            F++E+K + P AA       P  PEV P          H IW+ F + RFE+ ++ S +Q
Sbjct: 1397 FSAEMKEADPLAASEASQPKPCAPEVTP----------HYIWIDFLVQRFEIAKYCSSDQ 1456

Query: 1356 VLLFGRLLQGSTK-----PPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLE 1415
            V +F  LLQ S          + +RH AA G  F L+ LGL         N     T   
Sbjct: 1457 VEIFCSLLQRSLSLSIGGTAGSMNRHVAAIGPRFKLLTLGLSLLHADVLPN----ATIRN 1516

Query: 1416 LLEDRIYRASLGWFAHEPE-----------------------WYDVKHVNFAQ---SEAQ 1475
            +L ++IY  +  +F+  P                        + D K++  +Q    + Q
Sbjct: 1517 VLREKIYSTAFDYFSCPPRFPTQGEKRLREDISVMIKFWTAMFSDKKYLTASQLVPPDNQ 1576

Query: 1476 SVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKDQYHPVWGHLENYAVGREKRRQLL 1535
                 L      R    Q    T    +G+S ++ K                  KRR LL
Sbjct: 1577 DTRSNLDIAVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLL 1636

Query: 1536 LMLCQHEADRLEVWAQP--------------NIKESTPSRPKLSSEKWIEHVRTAFSVDP 1595
            L L   E +RL  W  P              ++         LS ++W ++V  A+S+ P
Sbjct: 1637 LSLLATEIERLITWYNPLSAPELELDQAGESSVANWRSKYISLSEKQWKDNVNLAWSISP 1696

Query: 1596 RIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHW 1655
             +A  + +RF    ++  E+ +LV+L    +  +PEA+ + VT   +D ++  L  +  W
Sbjct: 1697 HLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCW 1756

Query: 1656 AACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGY 1715
            A       L + +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALRYD+   V  Y
Sbjct: 1757 APADPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREY 1816

Query: 1716 LLRAAQRSDIFAHILIWHLQGETSVPDSG--KDANSGKNGSFLALLPVVRQHIIDGFTPK 1775
            +L AA +S + AH  IW+++    V + G  KD + G       LL  + + I    +  
Sbjct: 1817 ILWAASQSQLLAHQFIWNMKTNIYVDEEGHQKDPDIGD------LLEQLVEEITGSLSGP 1876

Query: 1776 ALDLFEREFNFFDKVTSISGVLFPLPK-DERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1835
            A D ++REF+FF+K+T++S ++ P PK DER+      L +++++    YLP+    +V 
Sbjct: 1877 AKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQ-PGCYLPSNPEAIVL 1936

Query: 1836 GIQVDSGIPLQSAAKVPIMITFNVVN-----------------------RDGDPNDIKPQ 1895
             I   SG P+QSAAK P +  F V                         ++ D   I  Q
Sbjct: 1937 DIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDPEEEGSMQEADGQKISWQ 1996

Query: 1896 ACIFKVGDDCRQDVLALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSR 1955
            A IFKVGDDCRQD+LALQ+I L ++IFQ VG++L++FPY V+ T PG G+IE +P+  SR
Sbjct: 1997 AAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSR 2056

Query: 1956 SQMGETTDGGLYEIFQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLI 2015
             Q+G  TD G+Y+ F + YG   +  F+ AR NFI S A Y++   LLQ KDRHNGN+++
Sbjct: 2057 DQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIML 2100

Query: 2016 DNTGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVK 2037
            D  G L+HIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+
Sbjct: 2117 DKKGHLIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVR 2100

BLAST of MC04g0487 vs. ExPASy Swiss-Prot
Match: P42356 (Phosphatidylinositol 4-kinase alpha OS=Homo sapiens OX=9606 GN=PI4KA PE=1 SV=4)

HSP 1 Score: 593.6 bits (1529), Expect = 8.6e-168
Identity = 473/1701 (27.81%), Postives = 756/1701 (44.44%), Query Frame = 0

Query: 456  SQGAMFESV----LKTCCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQAV 515
            SQ +M+E +    +   C  +++  T D   V+ F+     S+ +R    ++ DK+   +
Sbjct: 557  SQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFL----ASLSNRLYISQESDKDAHLI 616

Query: 516  PMQLNVIRLLAKMTVAVNKS-EVVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMASL 575
            P   + IR L  + VA+  + +V++ IL +    L++     P  L + ++D +  +   
Sbjct: 617  PD--HTIRALGHIAVALRDTPKVMEPILQI----LQQKFCQPPSPLDVLIIDQLGCLVIT 676

Query: 576  GFEKSYRETIVLMTRSYLSKLSSIGSSESRTLAPEANTERVETLPAGFLLIASGLKGAKL 635
            G +  Y+E   L  +  + K SS+  S ++             +      IA+ ++   L
Sbjct: 677  GNQYIYQEVWNLFQQISV-KASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHL 736

Query: 636  RSEYRHRLLSLCSDVGLSAESKSGR-----------SGADFLGPLLPAVAEICSDFDPTM 695
              E    LL L   +GL  +  S R           S A  LG L+P +A +     P  
Sbjct: 737  VDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIK 796

Query: 696  NIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLNSVGSMGAIALQAVSGPYMW 755
              +P L KLFR+ W Y  L G                               AV G  +W
Sbjct: 797  EAKPRLQKLFRDFWLYSVLMGF------------------------------AVEGSGLW 856

Query: 756  NTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSRQGSGNEKAAVAQRAALSAA 815
              +W   V  IA  +P L   S + L   L+ N+           N+    A+ + L + 
Sbjct: 857  PEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNS--------AMKNDTVTPAELSELRST 916

Query: 816  LGGRVD-----IAAMSTISGVKATYLLAVSFLEIIRFSSNGGVLNGGSNLNASRSAFSCV 875
            +   +D      A ++ +    +TYLL+V  LE +R      VL   S          C 
Sbjct: 917  IINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMR------VLR--STDPDRFQVMFCY 976

Query: 876  FEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETGNEAEVRDSTLFAHTCFLIRS 935
            FE          + QC+ A+    F+  ++ + D+     NE E     L  H  FL+ +
Sbjct: 977  FEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMADKAKTKENEEE-----LERHAQFLLVN 1036

Query: 936  MSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL-----------FSMHNDAPSAVV 995
             +   + +R +A   L+ L DKFP ++W+ + L ++L             +H D P   +
Sbjct: 1037 FNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDI 1096

Query: 996  TDPAWVVTVRSLYQGVVREWIIK------------SLSYAPCTSQGLLQEKLCKVNTWQR 1055
             D  + +TV   Y+   RE I+K            ++ +AP  ++  LQE L K   W  
Sbjct: 1097 PDAPYRITVPDTYE--ARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVS 1156

Query: 1056 --AQHTPDVISLLSEIRI-GTGKNDCWTG-IQTANIPAVMTAAAAASGADLKLTEAFNLE 1115
              +QHT   ++  S +   G  K +   G  Q +  PA +                   +
Sbjct: 1157 GLSQHTGLAMATESILHFAGYNKQNTTLGATQLSERPACVK------------------K 1216

Query: 1116 VLGTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKP 1175
                 M S  ++  +AGE+ GM R   +     TG  +       Q     +  + PQ  
Sbjct: 1217 DYSNFMASLNLRNRYAGEVYGMIRFSGT-----TGQMSDLNKMMVQDLHSALDRSHPQ-- 1276

Query: 1176 QNEDDSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNI 1235
                                                + +   + TA+L+S+   +     
Sbjct: 1277 -----------------------------------HYTQAMFKLTAMLISSKDCD----- 1336

Query: 1236 EGFARLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKR 1295
                +L+  LCW P  +     +ET +  W WL++    +    + E+  AW  T++ K 
Sbjct: 1337 ---PQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKDGVEVPFMREMAGAWHMTVEQKF 1396

Query: 1296 GLFASEVKYSGP-AAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSV 1355
            GLF++E+K + P AA       P  PEV P          H IW+ F + RFE+ ++ S 
Sbjct: 1397 GLFSAEIKEADPLAASEASQPKPCPPEVTP----------HYIWIDFLVQRFEIAKYCSS 1456

Query: 1356 EQVLLFGRLLQGSTK-----PPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTG 1415
            +QV +F  LLQ S          + +RH AA G  F L+ LGL         N     T 
Sbjct: 1457 DQVEIFSSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPN----ATI 1516

Query: 1416 LELLEDRIYRASLGWFAHEPE-----------------------WYDVKHVNFAQ---SE 1475
              +L ++IY  +  +F+  P+                       + D K++  +Q    +
Sbjct: 1517 RNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPD 1576

Query: 1476 AQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKDQYHPVWGHLENYAVGREKRRQ 1535
             Q     L      R    Q    T    +G+S ++ K                  KRR 
Sbjct: 1577 NQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRT 1636

Query: 1536 LLLMLCQHEADRLEVWAQP--------------NIKESTPSRPKLSSEKWIEHVRTAFSV 1595
            LLL L   E +RL  W  P              ++         LS ++W ++V  A+S+
Sbjct: 1637 LLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSI 1696

Query: 1596 DPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1655
             P +A  + +RF    ++  E+ +LV+L    +  +PEA+ + VT   +D ++  L  + 
Sbjct: 1697 SPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVL 1756

Query: 1656 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1715
             WA       L + +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALRYD+   V 
Sbjct: 1757 CWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVR 1816

Query: 1716 GYLLRAAQRSDIFAHILIWHLQGETSVPDSG--KDANSGKNGSFLALLPVVRQHIIDGFT 1775
             Y+L AA +S + AH  IW+++    + + G  KD + G       LL  + + I    +
Sbjct: 1817 EYILWAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGD------LLDQLVEEITGSLS 1876

Query: 1776 PKALDLFEREFNFFDKVTSISGVLFPLPK-DERRAGIRRELEKIEMEGEDLYLPTATNKL 1835
              A D ++REF+FF+K+T++S ++ P PK DER+      L +++++    YLP+    +
Sbjct: 1877 GPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQ-PGCYLPSNPEAI 1936

Query: 1836 VRGIQVDSGIPLQSAAKVPIMITFNV-----------------------VNRDGDPNDIK 1895
            V  I   SG P+QSAAK P +  F V                         ++ D   I 
Sbjct: 1937 VLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKIS 1996

Query: 1896 PQACIFKVGDDCRQDVLALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTR 1955
             QA IFKVGDDCRQD+LALQ+I L ++IFQ VG++L++FPY V+ T PG G+IE +P+  
Sbjct: 1997 WQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCT 2056

Query: 1956 SRSQMGETTDGGLYEIFQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNL 2015
            SR Q+G  TD G+Y+ F + YG   +  F+ AR NFI S A Y++   LLQ KDRHNGN+
Sbjct: 2057 SRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNI 2100

Query: 2016 LIDNTGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLC 2037
            ++D  G ++HIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +C
Sbjct: 2117 MLDKKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMC 2100

BLAST of MC04g0487 vs. ExPASy Swiss-Prot
Match: O08662 (Phosphatidylinositol 4-kinase alpha OS=Rattus norvegicus OX=10116 GN=Pi4ka PE=1 SV=2)

HSP 1 Score: 589.0 bits (1517), Expect = 2.1e-166
Identity = 469/1698 (27.62%), Postives = 758/1698 (44.64%), Query Frame = 0

Query: 456  SQGAMFESV----LKTCCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQAV 515
            +Q +M+E +    +   C  +++  T D   V+ F+     S+ +R    ++ DK+   +
Sbjct: 551  NQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFL----ASLSNRLYISQESDKDAHLI 610

Query: 516  PMQLNVIRLLAKMTVAVNKS-EVVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMASL 575
            P   + IR L  + VA+  + +V++ IL +    L++     P  L + ++D +  +   
Sbjct: 611  PD--HTIRALGHIAVALRDTPKVMEPILQI----LQQKFCQPPSPLDVLIIDQLGCLVIT 670

Query: 576  GFEKSYRETIVLMTRSYLSKLSSIGSSESRTLAPEANTERVETLPAGFLLIASGLKGAKL 635
            G +  Y+E   L  +  + K SS+  S ++             +      IA+ ++   L
Sbjct: 671  GNQYIYQEVWNLFQQISV-KASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHL 730

Query: 636  RSEYRHRLLSLCSDVGLSAESKSGR-----------SGADFLGPLLPAVAEICSDFDPTM 695
              E    LL L   +GL  +  S R           S A  LG L+P +A +     P  
Sbjct: 731  VDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIK 790

Query: 696  NIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLNSVGSMGAIALQAVSGPYMW 755
              +P L KLFR+ W Y  L G                               AV G  +W
Sbjct: 791  EAKPRLQKLFRDFWLYSVLMGF------------------------------AVEGSGLW 850

Query: 756  NTQWSSAVQFIAQGTPPLVVSSVKWLEDELELN-ALHNPGSRQGSGNEKAAVAQRAALSA 815
              +W   V  IA  +P L   S + L   L+ N A+ N        NE      R+ +  
Sbjct: 851  PEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELNE-----LRSTIIN 910

Query: 816  ALGGRVDIAAM-STISGVKATYLLAVSFLEIIRFSSNGGVLNGGSNLNASRSAFSCVFEY 875
             L    +++A+ + +    +TYLL+V  LE +R      VL   S          C FE 
Sbjct: 911  LLDPPPEVSALINKLDFAMSTYLLSVYRLEYMR------VLR--STDPDRFQVMFCYFED 970

Query: 876  LKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETGNEAEVRDSTLFAHTCFLIRSMSQ 935
                     + QC+ A+    F+  ++ + ++     NE E     L  H  FL+ + + 
Sbjct: 971  KAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEE-----LERHAQFLLVNFNH 1030

Query: 936  RDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL-----------FSMHNDAPSAVVTDP 995
              + +R +A   L+ L DKFP ++W+ + L ++L             +H D P   + D 
Sbjct: 1031 IHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDA 1090

Query: 996  AWVVTVRSLYQGVVREWIIK------------SLSYAPCTSQGLLQEKLCKVNTWQR--A 1055
             + +TV   Y+   RE I+K            ++ +AP  ++  LQE L K   W    +
Sbjct: 1091 PYRITVPDTYE--ARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLS 1150

Query: 1056 QHTPDVISLLSEIRI-GTGKNDCWTGI-QTANIPAVMTAAAAASGADLKLTEAFNLEVLG 1115
            QHT   ++  S +   G  K +   G+ Q    PA +                   +   
Sbjct: 1151 QHTGLAMATESILHFAGYNKQNTTLGVTQLTERPACVK------------------KDYS 1210

Query: 1116 TGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNE 1175
              M S  ++  +AGE+ GM R   + G                                +
Sbjct: 1211 NFMASLNLRNRYAGEVHGMIRFSGATG--------------------------------Q 1270

Query: 1176 DDSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGF 1235
                N +++   + +L                 + +   + TA+L+S+   +        
Sbjct: 1271 MSDLNKMMVQDLITALDH----------SHPQHYTQAMFKLTAMLISSKDCD-------- 1330

Query: 1236 ARLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLF 1295
             +L+  LCW P  +     +ET +  W WL++    +    + E+  AW  T++ K GLF
Sbjct: 1331 PQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGLF 1390

Query: 1296 ASEVKYSGP-AAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQV 1355
            ++E K + P AA       P  PEV P          H IW+ F + RFE+ ++ S +QV
Sbjct: 1391 SAETKEADPLAASEASQPRPCPPEVTP----------HYIWIDFLVQRFEIAKYCSSDQV 1450

Query: 1356 LLFGRLLQGSTK-----PPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLEL 1415
             +F  LLQ S          + +RH AA G  F L+ LGL         N     T   +
Sbjct: 1451 EIFSSLLQRSMSLHIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPN----ATIRNV 1510

Query: 1416 LEDRIYRASLGWFAHEPE-----------------------WYDVKHVNFAQ---SEAQS 1475
            L ++IY  +  +F+  P+                       + D K++  +Q    + Q 
Sbjct: 1511 LREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQD 1570

Query: 1476 VSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKDQYHPVWGHLENYAVGREKRRQLLL 1535
                L      R    Q    T    +G+S ++ K                  KRR LLL
Sbjct: 1571 TRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLL 1630

Query: 1536 MLCQHEADRLEVWAQP--------------NIKESTPSRPKLSSEKWIEHVRTAFSVDPR 1595
             L   E +RL  W  P              ++         LS ++W ++V  A+++ P 
Sbjct: 1631 SLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWTISPY 1690

Query: 1596 IAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHWA 1655
            +A  + +RF    ++  E+ +LV+L    +  +PEA+ + VT   +D ++  L  +  WA
Sbjct: 1691 LAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWA 1750

Query: 1656 ACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGYL 1715
                   L + +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALRYD+   V  Y+
Sbjct: 1751 PTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYI 1810

Query: 1716 LRAAQRSDIFAHILIWHLQGETSVPDSG--KDANSGKNGSFLALLPVVRQHIIDGFTPKA 1775
            L AA +S + AH  IW+++    + + G  KD + G       LL  + + I    +  A
Sbjct: 1811 LWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGD------LLEQLVEEITGSLSGPA 1870

Query: 1776 LDLFEREFNFFDKVTSISGVLFPLPK-DERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1835
             D ++REF+FF+K+T++S ++ P PK DER+      L +++++    YLP+    +V  
Sbjct: 1871 KDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQ-PGCYLPSNPEAIVLD 1930

Query: 1836 IQVDSGIPLQSAAKVPIMITFNVV-----------------------NRDGDPNDIKPQA 1895
            I   SG P+QSAAK P +  F V                         ++ D   I  QA
Sbjct: 1931 IDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLQCRSDTEDECRRQEADGKKICWQA 1990

Query: 1896 CIFKVGDDCRQDVLALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRS 1955
             IFKVGDDCRQD+LALQ+I L ++IFQ VG++L++FPY V+ T PG G+IE +P+  SR 
Sbjct: 1991 AIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRD 2050

Query: 1956 QMGETTDGGLYEIFQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLID 2015
            Q+G  TD G+Y+ F + YG   +  F+ AR NFI S A Y++   LLQ KDRHNGN+++D
Sbjct: 2051 QLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLD 2094

Query: 2016 NTGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKG 2037
              G ++HIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+G
Sbjct: 2111 KKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVRG 2094

BLAST of MC04g0487 vs. NCBI nr
Match: XP_022135807.1 (phosphatidylinositol 4-kinase alpha 1-like [Momordica charantia])

HSP 1 Score: 3962 bits (10276), Expect = 0.0
Identity = 2019/2047 (98.63%), Postives = 2021/2047 (98.73%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT
Sbjct: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120

Query: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180
            EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA
Sbjct: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180

Query: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240
            PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA
Sbjct: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240

Query: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300
            SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300

Query: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660
            TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF
Sbjct: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  QGSGNEKAAVAQRA----ALSAALGGRVDIAAMSTIS-----GVKATYLLAVSFLEIIRF 840
            QGSGNEKAA++        L       V     S IS     GVKATYLLAVSFLEIIRF
Sbjct: 781  QGSGNEKAALSVAYYIVYCLDVFFSRMVFSFLPSQISLFHFVGVKATYLLAVSFLEIIRF 840

Query: 841  SSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRIS 900
            SSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRIS
Sbjct: 841  SSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRIS 900

Query: 901  ETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL 960
            ETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL
Sbjct: 901  ETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL 960

Query: 961  FSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQR 1020
            FSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQR
Sbjct: 961  FSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQR 1020

Query: 1021 AQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGT 1080
            AQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGT
Sbjct: 1021 AQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGT 1080

Query: 1081 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNED 1140
            GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNED
Sbjct: 1081 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNED 1140

Query: 1141 DSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA 1200
            DSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA
Sbjct: 1141 DSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA 1200

Query: 1201 RLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA 1260
            RLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA
Sbjct: 1201 RLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA 1260

Query: 1261 SEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLL 1320
            SEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLL
Sbjct: 1261 SEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLL 1320

Query: 1321 FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYR 1380
            FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYR
Sbjct: 1321 FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYR 1380

Query: 1381 ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVN 1440
            ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVN
Sbjct: 1381 ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVN 1440

Query: 1441 SKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKW 1500
            SKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKW
Sbjct: 1441 SKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKW 1500

Query: 1501 IEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD 1560
            IEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD
Sbjct: 1501 IEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD 1560

Query: 1561 ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA 1620
            ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA
Sbjct: 1561 ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA 1620

Query: 1621 LRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVR 1680
            LRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVR
Sbjct: 1621 LRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVR 1680

Query: 1681 QHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYL 1740
            QHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYL
Sbjct: 1681 QHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYL 1740

Query: 1741 PTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDV 1800
            PTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDV
Sbjct: 1741 PTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDV 1800

Query: 1801 LALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI 1860
            LALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI
Sbjct: 1801 LALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI 1860

Query: 1861 FQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFI 1920
            FQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFI
Sbjct: 1861 FQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFI 1920

Query: 1921 LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIIN 1980
            LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIIN
Sbjct: 1921 LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIIN 1980

Query: 1981 TVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQY 2038
            TVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQY
Sbjct: 1981 TVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQY 2040

BLAST of MC04g0487 vs. NCBI nr
Match: XP_038887703.1 (phosphatidylinositol 4-kinase alpha 1-like [Benincasa hispida])

HSP 1 Score: 3816 bits (9896), Expect = 0.0
Identity = 1924/2038 (94.41%), Postives = 1986/2038 (97.45%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            M+ALIELCDLIAQNPALFSEKLSWICSRCPPSE  LAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            PDSVGLRPKSVV+EF+RSIPLSF+LSFWPQSYGNDAIASF+ EF+NYTSKACELST+FA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAM 120

Query: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180
            EISGF+SEVVLSAI+DC EGS ISRAFLVA+SKSF+PI+PSDADKLVSCILDRFLISEAA
Sbjct: 121  EISGFSSEVVLSAINDCCEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180

Query: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240
            PGTPRE  +ANSEASSSQ SPLSVSH P NGGLSPGNENGQVSGSLSSG SRITDDATSA
Sbjct: 181  PGTPREHNQANSEASSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300
            SSRGSGMMNG+S+LWKSG++QLGMN G SEGGGVA VRQQV  FEDESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLEQLGMNQGFSEGGGVAFVRQQVASFEDESIENLEKQEIAFK 300

Query: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            L+THILDNSSFDARLWEQMRAL KKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKMK V SLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMKTVVSLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480
            +QIAV+RGGQPLRVLLIRLKPLVLTVCAQADTWG+ QGAMFESVL TCCEIIESCWTKDR
Sbjct: 421  SQIAVSRGGQPLRVLLIRLKPLVLTVCAQADTWGN-QGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSE+VDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660
            T+APEA TERVE LPAGFLLIA+ LK AKLR EYRHRLLSLCSDVGL+AESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLLIANSLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVS+MLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720

Query: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGS  AIALQAVSGPY+WNTQWSSAVQFIA+GTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGST-AIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  QGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEIIRFSSNGGVLNG 840
            +GSGNEKAAVAQRAALSAALGGRVD+AAMSTISGVKATYLLAVSFLEIIRFSSNGG+LNG
Sbjct: 781  RGSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNG 840

Query: 841  GSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETGNEAEVR 900
            GSN+NASRSAF CVFEYLKTPNLLPAVSQCLTA+VH AFETAV WL+DRISETGNEAEVR
Sbjct: 841  GSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISETGNEAEVR 900

Query: 901  DSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPS 960
            DSTLFAHTC+LI+SMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPS
Sbjct: 901  DSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPS 960

Query: 961  AVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQRAQHTPDVIS 1020
             V++DPAWVVTVRSLYQ VVREWI+KSLSYAPCT QGLLQEKLCK NTWQR QHTPDVIS
Sbjct: 961  TVISDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRGQHTPDVIS 1020

Query: 1021 LLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGTGMVSATVKC 1080
            LLSEIRIGT KND WTGIQTANIPAV+TAAAAASGADLKLTEAFNLEVL TGMVSATVKC
Sbjct: 1021 LLSEIRIGTSKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKC 1080

Query: 1081 NHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNEDDSFNGILIM 1140
            NHAGEIAGMRRLYNSIGGFQTGVA PTGLGFGQG QRLI+GA PQ+PQNEDDSFNGILIM
Sbjct: 1081 NHAGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGAVPQQPQNEDDSFNGILIM 1140

Query: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFARLIRLLCWC 1200
            KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA+LIRLLCWC
Sbjct: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWC 1200

Query: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260
            PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA
Sbjct: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260

Query: 1261 AKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLLFGRLLQGST 1320
            AKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ+LLFGRLLQGST
Sbjct: 1261 AKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGST 1320

Query: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380
            KPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDR+YRASLGWFAHE
Sbjct: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRLYRASLGWFAHE 1380

Query: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKDQYHPVW 1440
            PEWYDVKHVNFAQS+AQSVSIFLHYLSS+RGN LQ DAK RGREN IS+V+S DQYHPVW
Sbjct: 1381 PEWYDVKHVNFAQSQAQSVSIFLHYLSSERGNSLQSDAKIRGRENSISLVDSNDQYHPVW 1440

Query: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKWIEHVRTAFS 1500
            GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLS+EKWIEH RTAFS
Sbjct: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSAEKWIEHARTAFS 1500

Query: 1501 VDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQL 1560
            VDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIR+IPEALPYFVTPKAVDENSELLRQL
Sbjct: 1501 VDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRTIPEALPYFVTPKAVDENSELLRQL 1560

Query: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV 1620
            PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV
Sbjct: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV 1620

Query: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVRQHIIDGFTP 1680
            EGYLLRAAQRSDIFAHILIWHLQGETS+PDSGKD NSGKNGSFLALLPVVRQHIIDGFTP
Sbjct: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTP 1680

Query: 1681 KALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740
            KALDLF+REF+FFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR
Sbjct: 1681 KALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIM+TFNVV+RDGDPN+IKPQACIFKVGDDCRQDVLALQVI+LL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLL 1800

Query: 1801 RDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDIFQAVG+NLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFILETSPGGNM 1920
            SP+FEAARENFIISSAGYAVASLLLQPKDRHNGNLL DN GRLVHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2038
            LPCFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of MC04g0487 vs. NCBI nr
Match: KAG7011695.1 (Phosphatidylinositol 4-kinase alpha 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 3795 bits (9841), Expect = 0.0
Identity = 1914/2038 (93.92%), Postives = 1974/2038 (96.86%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            M+ALIELCDLIAQNPALFSEKLSWICSRCPPSE  LAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            PDSVGLRPKSVV+EF+RSIPLSF+L+FWPQS+GNDAIASF+ EF+NYTSKACELST+FA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLAFWPQSFGNDAIASFFTEFLNYTSKACELSTDFAM 120

Query: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180
            E+SGFTSEVVLSAI DCSEGS ISRAFLVA+SKSF+PI+PSDADKLVSCILD FLISE  
Sbjct: 121  EVSGFTSEVVLSAISDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240
            PGTPREQI+ANSE SSSQ SPLSVSH P NGGLSPGNENGQVSGSLSSG SRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300
            SSRGSGMMNG+S+LWKSG+DQLGMNLG SE GGVA VRQQV  FEDESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSE-GGVAFVRQQVAFFEDESIENLEKQEIAFK 300

Query: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            L+THILDNSSFDARLWEQMRAL KKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKM  VASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480
            AQIAV+RGGQPLRVLLIRLKPL LTVCAQADTWGS+QGAMFESVL TCCEIIESCWTKDR
Sbjct: 421  AQIAVSRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKM+ AVNKSE+V+MILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMSAAVNKSEIVEMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVL+TRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESR 600

Query: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660
            T+APEA TERVE LPAGFL IASGLK AKLR EYRHRLL LCSDVGL+AESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGS  AIALQAVSGPY+WNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGST-AIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  QGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEIIRFSSNGGVLNG 840
            +GSGNEKAAVAQRAALSAALGGRVD+AAMSTISGVKATYLLAVSFLEIIRFSSNGG+LNG
Sbjct: 781  RGSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNG 840

Query: 841  GSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETGNEAEVR 900
            GSN+NA+RSAFSCVFEYLKTPNLLPAV+QCLTA+VH AFETAVSWL+DRISETGNEAEVR
Sbjct: 841  GSNVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVR 900

Query: 901  DSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPS 960
            DSTL+AHTC+LI+ MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA S
Sbjct: 901  DSTLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASS 960

Query: 961  AVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQRAQHTPDVIS 1020
             VV+DPAWVVTVRSLYQ VVREWI+KSLSYAPCT QGLLQEKLCK NTWQRAQHTPDVIS
Sbjct: 961  TVVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVIS 1020

Query: 1021 LLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGTGMVSATVKC 1080
            LLSEIRIGTGKND WTGIQTANIPAV+TAAAAASGADLKLTEAFNLEVL TGMVSATVKC
Sbjct: 1021 LLSEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKC 1080

Query: 1081 NHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNEDDSFNGILIM 1140
            NHAGEIAGMRRLYNSIGGFQTGVA PTGLGFGQG QRLI+G  PQ+PQNEDDSFNGILIM
Sbjct: 1081 NHAGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGTLPQQPQNEDDSFNGILIM 1140

Query: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFARLIRLLCWC 1200
            KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA+LIRLLCWC
Sbjct: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWC 1200

Query: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260
            PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA
Sbjct: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260

Query: 1261 AKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLLFGRLLQGST 1320
            AKLRPHLSPGEPE QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ+LLFGRLLQGST
Sbjct: 1261 AKLRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGST 1320

Query: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380
            KPPWNFSRHPAATGSFFTLMLLGLKF SCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE
Sbjct: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380

Query: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKDQYHPVW 1440
            PEWYDVKHVNFAQSEAQSVSIFLHYLSS+RGN LQ D KTRGRENGIS+V+S DQYHPVW
Sbjct: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDTKTRGRENGISLVDSNDQYHPVW 1440

Query: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKWIEHVRTAFS 1500
            GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKLS+EKWIEH RTAFS
Sbjct: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPVRPKLSAEKWIEHARTAFS 1500

Query: 1501 VDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQL 1560
            VDPRIAFSMVSRFPTN  LR E+ QLVQLHILDIRSIPEALPYF+TPKAVDENSELLRQL
Sbjct: 1501 VDPRIAFSMVSRFPTNTFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVDENSELLRQL 1560

Query: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV 1620
            PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLV
Sbjct: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLV 1620

Query: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVRQHIIDGFTP 1680
            EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKN SFLALLP+VR HIIDGFTP
Sbjct: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIIDGFTP 1680

Query: 1681 KALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740
            KA DLFEREF+FFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR
Sbjct: 1681 KARDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIMITFNVV+RDGDPN+IKPQACIFKVGDDCRQDVLALQVIALL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALL 1800

Query: 1801 RDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDIFQAVG+NLYLFPYGVLPTGPG+GIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDIFQAVGLNLYLFPYGVLPTGPGKGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFILETSPGGNM 1920
            SP+FEAARENFIISSAGYAVASLLLQPKDRHNGNLL DN GRLVHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2038
            LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of MC04g0487 vs. NCBI nr
Match: KAG6572023.1 (Phosphatidylinositol 4-kinase alpha 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3794 bits (9840), Expect = 0.0
Identity = 1914/2038 (93.92%), Postives = 1973/2038 (96.81%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            M+ALIELCDLIAQNPALFSEKLSWICSRCPPSE  LAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            PDSVGLRPKSVV+EF+RSIPLSF+L+FWPQS+GNDAIASF+ EF+NYTSKACELST+FA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLAFWPQSFGNDAIASFFTEFLNYTSKACELSTDFAM 120

Query: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180
            E+SGFTSEVVLSAI DCSEGS ISRAFLVA+SKSF+PI+PSDADKLVSCILD FLISE  
Sbjct: 121  EVSGFTSEVVLSAISDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240
            PGTPREQI+ANSE SSSQ SPLSVSH P NGGLSPGNENGQVSGSLSSG SRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300
            SSRGSGMMNG+S+LWKSG+DQLGMNLG SE GGVA VRQQV  FEDESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSE-GGVAFVRQQVAFFEDESIENLEKQEIAFK 300

Query: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            L+THILDNSSFDARLWEQMRAL KKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKM  VASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480
            AQIAV RGGQPLRVLLIRLKPL LTVCAQADTWGS+QGAMFESVL TCCEIIESCWTKDR
Sbjct: 421  AQIAVTRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKM+ AVNKSE+V+MILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMSAAVNKSEIVEMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVL+TRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESR 600

Query: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660
            T+APEA TERVE LPAGFL IASGLK AKLR EYRHRLL LCSDVGL+AESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGS  AIALQAVSGPY+WNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGST-AIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  QGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEIIRFSSNGGVLNG 840
            +GSGNEKAAVAQRAALSAALGGRVD+AAMSTISGVKATYLLAVSFLEIIRFSSNGG+LNG
Sbjct: 781  RGSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNG 840

Query: 841  GSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETGNEAEVR 900
            GSN+NA+RSAFSCVFEYLKTPNLLPAV+QCLTA+VH AFETAVSWL+DRISETGNEAEVR
Sbjct: 841  GSNVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVR 900

Query: 901  DSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPS 960
            DSTL+AHTC+LI+ MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA S
Sbjct: 901  DSTLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASS 960

Query: 961  AVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQRAQHTPDVIS 1020
             VV+DPAWVVTVRSLYQ VVREWI+KSLSYAPCT QGLLQEKLCK NTWQRAQHTPDVIS
Sbjct: 961  TVVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVIS 1020

Query: 1021 LLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGTGMVSATVKC 1080
            LLSEIRIGTGKND WTGIQTANIPAV+TAAAAASGADLKLTEAFNLEVL TGMVSATVKC
Sbjct: 1021 LLSEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKC 1080

Query: 1081 NHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNEDDSFNGILIM 1140
            NHAGEIAGMRRLYNSIGGFQTGVA PTGLGFGQG QRLI+G  PQ+PQNEDDSFNGILIM
Sbjct: 1081 NHAGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGTLPQQPQNEDDSFNGILIM 1140

Query: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFARLIRLLCWC 1200
            KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA+LIRLLCWC
Sbjct: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWC 1200

Query: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260
            PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA
Sbjct: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260

Query: 1261 AKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLLFGRLLQGST 1320
            AKLRPHLSPGEPE QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ+LLFGRLLQGST
Sbjct: 1261 AKLRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGST 1320

Query: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380
            KPPWNFSRHPAATGSFFTLMLLGLKF SCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE
Sbjct: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380

Query: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKDQYHPVW 1440
            PEWYDVKHVNFAQSEAQSVSIFLHYLSS+RGN LQ D KTRGRENGIS+V+S DQYHPVW
Sbjct: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDTKTRGRENGISLVDSNDQYHPVW 1440

Query: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKWIEHVRTAFS 1500
            GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKLS+EKWIEH RTAFS
Sbjct: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPVRPKLSAEKWIEHARTAFS 1500

Query: 1501 VDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQL 1560
            VDPRIAFSMVSRFPTN  LR E+ QLVQLHILDIRSIPEALPYF+TPKAVDENSELLRQL
Sbjct: 1501 VDPRIAFSMVSRFPTNTFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVDENSELLRQL 1560

Query: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV 1620
            PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLV
Sbjct: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLV 1620

Query: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVRQHIIDGFTP 1680
            EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKN SFLALLP+VR HIIDGFTP
Sbjct: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIIDGFTP 1680

Query: 1681 KALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740
            KA DLFEREF+FFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR
Sbjct: 1681 KARDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIMITFNVV+RDGDPN+IKPQACIFKVGDDCRQDVLALQVIALL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALL 1800

Query: 1801 RDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDIFQAVG+NLYLFPYGVLPTGPG+GIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDIFQAVGLNLYLFPYGVLPTGPGKGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFILETSPGGNM 1920
            SP+FEAARENFIISSAGYAVASLLLQPKDRHNGNLL DN GRLVHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2038
            LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of MC04g0487 vs. NCBI nr
Match: XP_022952154.1 (phosphatidylinositol 4-kinase alpha 1-like [Cucurbita moschata])

HSP 1 Score: 3793 bits (9835), Expect = 0.0
Identity = 1912/2038 (93.82%), Postives = 1974/2038 (96.86%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            M+ALIELCDLIAQNPALFSEKLSWICSRCPPSE  LAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            PDSVGLRPKSVV+EF+RSIPLSF+LSFWPQS+GNDAIASF+ EF+NYTS+ACELST+FA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSFGNDAIASFFTEFLNYTSQACELSTDFAM 120

Query: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180
            E+SGFTSEVVLSAI DCSEGS IS AFLVA+SKSF+PI+PSDADKLVSCILD FLISE  
Sbjct: 121  EVSGFTSEVVLSAISDCSEGSAISSAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240
            PGTPREQI+ANSE SSSQ SPLSVSH PLNGGLSPGNENGQVSGSLSSG SRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPLNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300
            SSRGSGMMNG+S+LWKSG+DQLGMNLG SE GG+A VRQQV  FEDESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSE-GGLAFVRQQVAFFEDESIENLEKQEIAFK 300

Query: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            L+THILDNSSFDARLWEQMRAL KKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKM  VASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480
            AQIAV RGGQPLRVLLIRLKPL LTVCAQADTWGS+QGAMFESVL TCCEIIESCWTKDR
Sbjct: 421  AQIAVTRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKM+ AVNKSE+V+MILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMSAAVNKSEIVEMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVL+TRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESR 600

Query: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660
            T+APEA TERVE LPAGFL IASGLK AKLR EYRHRLL LCSDVGL+AESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGS  AIALQAVSGPY+WNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGST-AIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  QGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEIIRFSSNGGVLNG 840
            +GSGNEKAAVAQRAALSAALGGRVD+AAMSTISGVKATYLLAVSFLEIIRFSSNGG+LNG
Sbjct: 781  RGSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNG 840

Query: 841  GSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETGNEAEVR 900
            GSN+NA+RSAFSCVFEYLKTPNLLPAV+QCLTA+VH AFETAVSWL+DRISETGNEAEVR
Sbjct: 841  GSNVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVR 900

Query: 901  DSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPS 960
            DSTL+AHTC+LI+ MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA S
Sbjct: 901  DSTLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASS 960

Query: 961  AVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQRAQHTPDVIS 1020
             VV+DPAWVVTVRSLYQ VVREWI+KSLSYAPCT QGLLQEKLCK NTWQRAQHTPDVIS
Sbjct: 961  TVVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVIS 1020

Query: 1021 LLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGTGMVSATVKC 1080
            LLSEIRIGTGKND WTGIQTANIPAV+TAAAAASGADLKLTEAFNLEVL TGMVSATVKC
Sbjct: 1021 LLSEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKC 1080

Query: 1081 NHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNEDDSFNGILIM 1140
            NHAGEIAGMRRLYNSIGGFQTGVA PTGLGFGQG QRLI+G  PQ+PQNEDDSFNGILIM
Sbjct: 1081 NHAGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGTLPQQPQNEDDSFNGILIM 1140

Query: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFARLIRLLCWC 1200
            KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA+LIRLLCWC
Sbjct: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWC 1200

Query: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260
            PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA
Sbjct: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260

Query: 1261 AKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLLFGRLLQGST 1320
            AKLRPHLSPGEPE QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ+LLFGRLLQGST
Sbjct: 1261 AKLRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGST 1320

Query: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380
            KPPWNFSRHPAATGSFFTLMLLGLKF SCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE
Sbjct: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380

Query: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKDQYHPVW 1440
            PEWYDVKHVNFAQSEAQSVSIFLHYLSS+RGN LQ D KTRGRENGIS+V+S DQYHPVW
Sbjct: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDTKTRGRENGISLVDSNDQYHPVW 1440

Query: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKWIEHVRTAFS 1500
            GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP+RPKLS+EKWIEH RTAFS
Sbjct: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPARPKLSAEKWIEHARTAFS 1500

Query: 1501 VDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQL 1560
            VDPRIAFSMVSRFPTN  LR E+ QLVQLHILDIRSIPEALPYF+TPKAV+ENSELLRQL
Sbjct: 1501 VDPRIAFSMVSRFPTNGFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVEENSELLRQL 1560

Query: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV 1620
            PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLV
Sbjct: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLV 1620

Query: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVRQHIIDGFTP 1680
            EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKN SFLALLP+VR HIIDGFTP
Sbjct: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIIDGFTP 1680

Query: 1681 KALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740
            KA DLFEREF+FFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR
Sbjct: 1681 KARDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIMITFNVV+RDGDPN+IKPQACIFKVGDDCRQDVLALQVIALL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALL 1800

Query: 1801 RDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDIFQAVG+NLYLFPYGVLPTGPG+GIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDIFQAVGLNLYLFPYGVLPTGPGKGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFILETSPGGNM 1920
            SP+FEAARENFIISSAGYAVASLLLQPKDRHNGNLL DN GRLVHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2038
            LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of MC04g0487 vs. ExPASy TrEMBL
Match: A0A6J1C3T4 (phosphatidylinositol 4-kinase alpha 1-like OS=Momordica charantia OX=3673 GN=LOC111007670 PE=4 SV=1)

HSP 1 Score: 3962 bits (10276), Expect = 0.0
Identity = 2019/2047 (98.63%), Postives = 2021/2047 (98.73%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT
Sbjct: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120

Query: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180
            EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA
Sbjct: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180

Query: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240
            PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA
Sbjct: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240

Query: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300
            SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300

Query: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660
            TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF
Sbjct: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  QGSGNEKAAVAQRA----ALSAALGGRVDIAAMSTIS-----GVKATYLLAVSFLEIIRF 840
            QGSGNEKAA++        L       V     S IS     GVKATYLLAVSFLEIIRF
Sbjct: 781  QGSGNEKAALSVAYYIVYCLDVFFSRMVFSFLPSQISLFHFVGVKATYLLAVSFLEIIRF 840

Query: 841  SSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRIS 900
            SSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRIS
Sbjct: 841  SSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRIS 900

Query: 901  ETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL 960
            ETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL
Sbjct: 901  ETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL 960

Query: 961  FSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQR 1020
            FSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQR
Sbjct: 961  FSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQR 1020

Query: 1021 AQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGT 1080
            AQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGT
Sbjct: 1021 AQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGT 1080

Query: 1081 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNED 1140
            GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNED
Sbjct: 1081 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNED 1140

Query: 1141 DSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA 1200
            DSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA
Sbjct: 1141 DSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA 1200

Query: 1201 RLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA 1260
            RLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA
Sbjct: 1201 RLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA 1260

Query: 1261 SEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLL 1320
            SEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLL
Sbjct: 1261 SEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLL 1320

Query: 1321 FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYR 1380
            FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYR
Sbjct: 1321 FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYR 1380

Query: 1381 ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVN 1440
            ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVN
Sbjct: 1381 ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVN 1440

Query: 1441 SKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKW 1500
            SKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKW
Sbjct: 1441 SKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKW 1500

Query: 1501 IEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD 1560
            IEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD
Sbjct: 1501 IEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD 1560

Query: 1561 ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA 1620
            ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA
Sbjct: 1561 ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA 1620

Query: 1621 LRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVR 1680
            LRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVR
Sbjct: 1621 LRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVR 1680

Query: 1681 QHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYL 1740
            QHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYL
Sbjct: 1681 QHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYL 1740

Query: 1741 PTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDV 1800
            PTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDV
Sbjct: 1741 PTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDV 1800

Query: 1801 LALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI 1860
            LALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI
Sbjct: 1801 LALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI 1860

Query: 1861 FQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFI 1920
            FQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFI
Sbjct: 1861 FQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFI 1920

Query: 1921 LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIIN 1980
            LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIIN
Sbjct: 1921 LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIIN 1980

Query: 1981 TVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQY 2038
            TVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQY
Sbjct: 1981 TVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQY 2040

BLAST of MC04g0487 vs. ExPASy TrEMBL
Match: A0A6J1GKT2 (phosphatidylinositol 4-kinase alpha 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454915 PE=4 SV=1)

HSP 1 Score: 3793 bits (9835), Expect = 0.0
Identity = 1912/2038 (93.82%), Postives = 1974/2038 (96.86%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            M+ALIELCDLIAQNPALFSEKLSWICSRCPPSE  LAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            PDSVGLRPKSVV+EF+RSIPLSF+LSFWPQS+GNDAIASF+ EF+NYTS+ACELST+FA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSFGNDAIASFFTEFLNYTSQACELSTDFAM 120

Query: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180
            E+SGFTSEVVLSAI DCSEGS IS AFLVA+SKSF+PI+PSDADKLVSCILD FLISE  
Sbjct: 121  EVSGFTSEVVLSAISDCSEGSAISSAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240
            PGTPREQI+ANSE SSSQ SPLSVSH PLNGGLSPGNENGQVSGSLSSG SRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPLNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300
            SSRGSGMMNG+S+LWKSG+DQLGMNLG SE GG+A VRQQV  FEDESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSE-GGLAFVRQQVAFFEDESIENLEKQEIAFK 300

Query: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            L+THILDNSSFDARLWEQMRAL KKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKM  VASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480
            AQIAV RGGQPLRVLLIRLKPL LTVCAQADTWGS+QGAMFESVL TCCEIIESCWTKDR
Sbjct: 421  AQIAVTRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKM+ AVNKSE+V+MILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMSAAVNKSEIVEMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVL+TRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESR 600

Query: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660
            T+APEA TERVE LPAGFL IASGLK AKLR EYRHRLL LCSDVGL+AESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGS  AIALQAVSGPY+WNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGST-AIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  QGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEIIRFSSNGGVLNG 840
            +GSGNEKAAVAQRAALSAALGGRVD+AAMSTISGVKATYLLAVSFLEIIRFSSNGG+LNG
Sbjct: 781  RGSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNG 840

Query: 841  GSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETGNEAEVR 900
            GSN+NA+RSAFSCVFEYLKTPNLLPAV+QCLTA+VH AFETAVSWL+DRISETGNEAEVR
Sbjct: 841  GSNVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVR 900

Query: 901  DSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPS 960
            DSTL+AHTC+LI+ MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA S
Sbjct: 901  DSTLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASS 960

Query: 961  AVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQRAQHTPDVIS 1020
             VV+DPAWVVTVRSLYQ VVREWI+KSLSYAPCT QGLLQEKLCK NTWQRAQHTPDVIS
Sbjct: 961  TVVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVIS 1020

Query: 1021 LLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGTGMVSATVKC 1080
            LLSEIRIGTGKND WTGIQTANIPAV+TAAAAASGADLKLTEAFNLEVL TGMVSATVKC
Sbjct: 1021 LLSEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKC 1080

Query: 1081 NHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNEDDSFNGILIM 1140
            NHAGEIAGMRRLYNSIGGFQTGVA PTGLGFGQG QRLI+G  PQ+PQNEDDSFNGILIM
Sbjct: 1081 NHAGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGTLPQQPQNEDDSFNGILIM 1140

Query: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFARLIRLLCWC 1200
            KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA+LIRLLCWC
Sbjct: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWC 1200

Query: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260
            PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA
Sbjct: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260

Query: 1261 AKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLLFGRLLQGST 1320
            AKLRPHLSPGEPE QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ+LLFGRLLQGST
Sbjct: 1261 AKLRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGST 1320

Query: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380
            KPPWNFSRHPAATGSFFTLMLLGLKF SCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE
Sbjct: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380

Query: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKDQYHPVW 1440
            PEWYDVKHVNFAQSEAQSVSIFLHYLSS+RGN LQ D KTRGRENGIS+V+S DQYHPVW
Sbjct: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDTKTRGRENGISLVDSNDQYHPVW 1440

Query: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKWIEHVRTAFS 1500
            GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP+RPKLS+EKWIEH RTAFS
Sbjct: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPARPKLSAEKWIEHARTAFS 1500

Query: 1501 VDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQL 1560
            VDPRIAFSMVSRFPTN  LR E+ QLVQLHILDIRSIPEALPYF+TPKAV+ENSELLRQL
Sbjct: 1501 VDPRIAFSMVSRFPTNGFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVEENSELLRQL 1560

Query: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV 1620
            PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLV
Sbjct: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLV 1620

Query: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVRQHIIDGFTP 1680
            EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKN SFLALLP+VR HIIDGFTP
Sbjct: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIIDGFTP 1680

Query: 1681 KALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740
            KA DLFEREF+FFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR
Sbjct: 1681 KARDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIMITFNVV+RDGDPN+IKPQACIFKVGDDCRQDVLALQVIALL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALL 1800

Query: 1801 RDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDIFQAVG+NLYLFPYGVLPTGPG+GIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDIFQAVGLNLYLFPYGVLPTGPGKGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFILETSPGGNM 1920
            SP+FEAARENFIISSAGYAVASLLLQPKDRHNGNLL DN GRLVHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2038
            LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of MC04g0487 vs. ExPASy TrEMBL
Match: A0A1S3BGG8 (phosphatidylinositol 4-kinase alpha 1-like OS=Cucumis melo OX=3656 GN=LOC103489597 PE=4 SV=1)

HSP 1 Score: 3792 bits (9833), Expect = 0.0
Identity = 1905/2038 (93.47%), Postives = 1978/2038 (97.06%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            M+ALIELCDLIAQNPALFSEKLSWICSRCPPSE  LAGSP ISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPTISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            PDSVGLRPKSVV+EF+RSIPLSF+LSFWPQSYG+DAIASF+ EF+NYTSKACELST+FAT
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGHDAIASFFNEFLNYTSKACELSTDFAT 120

Query: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180
            E+SGF+SEVVLSAI+DCSEGS ISRAFLVA++KSF+PI+PSDADKLVSCILDRFLISEA 
Sbjct: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALTKSFLPIIPSDADKLVSCILDRFLISEAI 180

Query: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240
            P TPRE  +ANSE SSSQ SPLSVSH P NGGLSPGNENGQVSGSLSSG SRITDDATSA
Sbjct: 181  PETPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300
            SSRGSGMMNG+S+LWKSG++QLGMNLG SEGGGVA VRQQV +FEDESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFEDESIENLEKQEIAFK 300

Query: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            L+THILDNSSFDARLWEQM+ L KKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMKTLAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKMK VASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVC QADTWG++QGAMFESVL TCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKMTVAVNKSE+VDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660
            T+APEA TERVE LPAGFL IA+GLK AKLR EYRHRLLSLCSDVGL+AESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLHIANGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVS+MLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720

Query: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGS  AIALQAVSGPY+WNTQWSSAVQFIA+GTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGST-AIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  QGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEIIRFSSNGGVLNG 840
            +GSGNEKAA+AQRAALSAALGGRVD+AAMSTISGVKATYLLAVSFLEIIRFSSNGG+LNG
Sbjct: 781  RGSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNG 840

Query: 841  GSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETGNEAEVR 900
            GSN+NASRSAF CVFEYLKTPNLLPAVSQCLTA+VH AFETAVSWL+DRIS+TGNEAE R
Sbjct: 841  GSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEAR 900

Query: 901  DSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPS 960
            DSTLFAHTC+LI+SMSQRDEHVRDIAVNLLTQLR+KFPQVMWNSSCLDSLLFSMHNDAPS
Sbjct: 901  DSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLREKFPQVMWNSSCLDSLLFSMHNDAPS 960

Query: 961  AVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQRAQHTPDVIS 1020
             VV+DPAWVVTVRSLYQ VVREWI+KSLSYAPCT QGLLQEKLCK NTWQRAQHTPDVIS
Sbjct: 961  TVVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVIS 1020

Query: 1021 LLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGTGMVSATVKC 1080
            LLSEIRIGT KN+ WTG QTANIPAV+TAAAAASGADLKLTEAFNLEVL TGMVSATVKC
Sbjct: 1021 LLSEIRIGTSKNEHWTGTQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKC 1080

Query: 1081 NHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNEDDSFNGILIM 1140
            NHAGEIAGMRRLYNSIGGFQTGVA  TGLGFGQG QRLI+GA PQ+PQNEDDSFNGILIM
Sbjct: 1081 NHAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIM 1140

Query: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFARLIRLLCWC 1200
            KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNL SESKTNIEGFA+LIRLLCWC
Sbjct: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLDSESKTNIEGFAQLIRLLCWC 1200

Query: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260
            PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFAS++KYSGPA
Sbjct: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDIKYSGPA 1260

Query: 1261 AKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLLFGRLLQGST 1320
            A LRPHLSPGEPE+QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ+LLFGRLLQGST
Sbjct: 1261 AMLRPHLSPGEPEMQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGST 1320

Query: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380
            KPPWNFSRHPAATGSFFTLMLLGLKFCSCQ+QGNLQNFKTGLELLEDRIYRASLGWFAHE
Sbjct: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380

Query: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKDQYHPVW 1440
            PEWYDVKHVNFAQSEAQSVSIFLHYLSS+RGN LQ DAK RGRENGIS+++S D YHPVW
Sbjct: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKIRGRENGISLIDSNDHYHPVW 1440

Query: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKWIEHVRTAFS 1500
            GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKL++EKWIEH RTAFS
Sbjct: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPYRPKLTAEKWIEHARTAFS 1500

Query: 1501 VDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQL 1560
            VDPRIAFSMVSRFPTNA LRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQL
Sbjct: 1501 VDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQL 1560

Query: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV 1620
            PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV
Sbjct: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV 1620

Query: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVRQHIIDGFTP 1680
            EGYLLRAA+RSDIFAHILIWHLQGETS PDSGKD NSGKNGSFLALLPVVRQHIIDGFTP
Sbjct: 1621 EGYLLRAAKRSDIFAHILIWHLQGETSSPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTP 1680

Query: 1681 KALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740
            KALDLF REF+FFDKVTSISGVLFPLPKDERRAGIRRELEKIEM+GEDLYLPTATNKLVR
Sbjct: 1681 KALDLFRREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMDGEDLYLPTATNKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIM+TFNVV+RDGDPN+IKPQACIFKVGDDCRQDVLALQVI+LL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLL 1800

Query: 1801 RDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDIFQAVG+NLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFILETSPGGNM 1920
            SPNFEAARENFI+SSAGYAVASLLLQPKDRHNGNLL DN GRLVHIDFGFILETSPGGNM
Sbjct: 1861 SPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2038
            LPCFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2037

BLAST of MC04g0487 vs. ExPASy TrEMBL
Match: A0A6J1I6A0 (phosphatidylinositol 4-kinase alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471091 PE=4 SV=1)

HSP 1 Score: 3787 bits (9820), Expect = 0.0
Identity = 1910/2038 (93.72%), Postives = 1973/2038 (96.81%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            M+ALIELCDLIAQNPALFSEKLSWICSRCPPSE  LAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            P+SVGLRPKSVV+EF+RSIPLSF+LSFWPQS+GNDAIASF+ EF+NYTSKACELST+FA 
Sbjct: 61   PESVGLRPKSVVLEFLRSIPLSFSLSFWPQSFGNDAIASFFTEFLNYTSKACELSTDFAM 120

Query: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180
            E+SGFTSEVVLSAI+DCSEGS ISRAFLVA+SKSF+PI+PSDADKLVSCILD FLISE  
Sbjct: 121  EVSGFTSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240
            PGTPREQI+ANSE SSSQ SPLSVSH P NGGLSPGNENGQVSGSLSSG SRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300
            SSRGSGMMNG+S+LWKSG+DQLGMNLG SE GGVA VRQQV  FEDESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSE-GGVAFVRQQVAFFEDESIENLEKQEIAFK 300

Query: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            L+THILDNSSFDARLWEQMRAL KKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKM  VASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480
            AQIAV RGGQPLRVLLIRLKPL LTVCAQADTWGS+QGAMFESVL TCCEIIESCWTKDR
Sbjct: 421  AQIAVTRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQAVP QLNVIRLLAKM+ AVNKSE+V+MILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPTQLNVIRLLAKMSAAVNKSEIVEMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVL+TRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESR 600

Query: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660
            T+APEA TERVE LPAGFL IASGLK AKLR EYRHRLL LCSDVGL+AESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGS  AIALQAVSGPY+WNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGST-AIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  QGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEIIRFSSNGGVLNG 840
            +GSGNEKAAVAQRAALSAALGGRVD+AAMSTISGVKATYLLAVSFLEIIRFSSNGG+LNG
Sbjct: 781  RGSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNG 840

Query: 841  GSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETGNEAEVR 900
            GSN+NA+RSAFSCVFEYLKTPNLLPAV+QCLTA+VH AFETAVSWL+DRISETGNEAEVR
Sbjct: 841  GSNVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVR 900

Query: 901  DSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPS 960
            DSTL+AHTC+LI+ MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA S
Sbjct: 901  DSTLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASS 960

Query: 961  AVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQRAQHTPDVIS 1020
             VV+DP+WVVTVRSLYQ VVREWI+KSLSYAPCT QGLLQEKLCK NTWQRAQHTPDVIS
Sbjct: 961  TVVSDPSWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVIS 1020

Query: 1021 LLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGTGMVSATVKC 1080
            LLSEIRIGTGKND WTGIQTANIPAV+TAAAAASGADLKLTEAFNLEVL TGMVSATVKC
Sbjct: 1021 LLSEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKC 1080

Query: 1081 NHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNEDDSFNGILIM 1140
            NHAGEIAGMRRLYNSIGGFQTGV  PTGLGFGQG QRLI+GA PQ+ QNEDDSFNGILIM
Sbjct: 1081 NHAGEIAGMRRLYNSIGGFQTGVTGPTGLGFGQGLQRLITGALPQQSQNEDDSFNGILIM 1140

Query: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFARLIRLLCWC 1200
            KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASE+KTNIEGFA+LIRLLCWC
Sbjct: 1141 KFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASEAKTNIEGFAQLIRLLCWC 1200

Query: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260
            PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA
Sbjct: 1201 PAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPA 1260

Query: 1261 AKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLLFGRLLQGST 1320
            AKLRPHLSPGEPE QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ+LLFGRLLQGST
Sbjct: 1261 AKLRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGST 1320

Query: 1321 KPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380
            KPPWNFS HPAATGSFFTLMLLGLKF SCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE
Sbjct: 1321 KPPWNFSHHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHE 1380

Query: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKDQYHPVW 1440
            PEWYDVKHVNFAQSEAQSVSIFLHYLSS+RGN LQ DAKTRGRENGIS+V+S DQYHPVW
Sbjct: 1381 PEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKTRGRENGISLVDSNDQYHPVW 1440

Query: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKWIEHVRTAFS 1500
            GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP+RPKLS+EKWIEH RTAFS
Sbjct: 1441 GHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPARPKLSAEKWIEHARTAFS 1500

Query: 1501 VDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQL 1560
            VDPRIAFSMVSRFPTN  LR E+ QLVQLHILDIRSIPEALPYF+TPKAVDENSELLRQL
Sbjct: 1501 VDPRIAFSMVSRFPTNVFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVDENSELLRQL 1560

Query: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLV 1620
            PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLV
Sbjct: 1561 PHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLV 1620

Query: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVRQHIIDGFTP 1680
            EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKN SFLALLP+VR HII+GFTP
Sbjct: 1621 EGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIINGFTP 1680

Query: 1681 KALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740
            KA DLFEREF+FFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR
Sbjct: 1681 KARDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIMITFNVV+RDGDPN+IKPQACIFKVGDDCRQDVLALQVIALL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALL 1800

Query: 1801 RDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDIFQAVG+NLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFILETSPGGNM 1920
            SP+FEAARENFIISSAGYAVASLLLQPKDRHNGNLL DN GRLVHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2038
            LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of MC04g0487 vs. ExPASy TrEMBL
Match: A0A6P3YUH8 (phosphatidylinositol 4-kinase alpha 1 OS=Ziziphus jujuba OX=326968 GN=LOC107404942 PE=4 SV=1)

HSP 1 Score: 3301 bits (8559), Expect = 0.0
Identity = 1662/2045 (81.27%), Postives = 1837/2045 (89.83%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            MEALIELCDLIAQNP  FSEKLSWIC RCPP E  L GSP +SRSQLNAVLAVAR LSKC
Sbjct: 1    MEALIELCDLIAQNPVQFSEKLSWICGRCPPPESLLEGSPRVSRSQLNAVLAVARFLSKC 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
             DS  LRPKSVV+EF+R IP SFN SFWPQS+GNDAIASF+A+F+ Y SKA ELS +FAT
Sbjct: 61   QDSADLRPKSVVLEFLRFIPASFNQSFWPQSFGNDAIASFFADFLGYVSKAPELSPDFAT 120

Query: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEA- 180
            EI+GFT +VV+SAI + S    ISR FL A+S+SF PI  SDA++LV+ +LD+   S   
Sbjct: 121  EIAGFTGDVVVSAISNASADLGISRVFLTALSQSFPPISASDAERLVNLLLDQLAASGTI 180

Query: 181  ---APGTPREQIRANSEASSSQGSPLSVSH-HPLNGGLSPGNENGQVSGSLSSGGSRITD 240
               +P TPREQI ANSE SSSQ SPLS++H  P  G  SPGNE   VSGS  S  SRI D
Sbjct: 181  VAQSPATPREQITANSETSSSQSSPLSLNHSQPHGGSSSPGNEASNVSGSSGSAASRIAD 240

Query: 241  DATSASSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQ 300
            DATSASSRGS MMNG S+LWKSGVDQLG+N G ++GGG   +RQQV+ FE+ES+E+LEKQ
Sbjct: 241  DATSASSRGSMMMNGGSILWKSGVDQLGVNFGYNDGGGAMLLRQQVSSFEEESVESLEKQ 300

Query: 301  EIAFKLVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTK 360
            EIAFKL+ HIL+    D  L EQ+R + K+QLQ+L  FLKI+KRDW+E G++LKARINTK
Sbjct: 301  EIAFKLIAHILEKCCIDMGLLEQVRFIAKRQLQSLTVFLKIRKRDWNEHGTLLKARINTK 360

Query: 361  LLVYQAAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGS 420
            LLVY+AAA+L +K +     DGK AKKL  ET AL MDAAE+CLLSVWRKLRICEELFGS
Sbjct: 361  LLVYKAAAKLTLKTLDCFHSDGKLAKKLAHETLALFMDAAESCLLSVWRKLRICEELFGS 420

Query: 421  LLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESC 480
            LL GL+QIAV RGGQ LR+LLIRLKP++LTVC QADTW +SQGAMFESV+KT C+IIESC
Sbjct: 421  LLAGLSQIAVHRGGQSLRILLIRLKPVILTVCTQADTWATSQGAMFESVMKTTCQIIESC 480

Query: 481  WTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQAVP-MQLNVIRLLAKMTVAVNKSEVV 540
            W K+RAPVDTFIMGLATSIR+RND EEQ DK+K+ +P MQLNVIRLLA + VAVNKSEVV
Sbjct: 481  WAKERAPVDTFIMGLATSIRERNDYEEQVDKDKETIPVMQLNVIRLLADLNVAVNKSEVV 540

Query: 541  DMILPLFIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSI 600
            DMILPLFIESLEEGDASTP LLRL+LLDAVSRMASLGFEKSYRET+VLMTRSYL+KLSS+
Sbjct: 541  DMILPLFIESLEEGDASTPSLLRLRLLDAVSRMASLGFEKSYRETVVLMTRSYLNKLSSV 600

Query: 601  GSSESRTLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSG 660
            GS+ES+T+A EA TERVETLP+GF LIASGL   KLR++YRHRLLSLCSDVGL+AESKSG
Sbjct: 601  GSAESKTVAAEATTERVETLPSGFHLIASGLTNTKLRADYRHRLLSLCSDVGLAAESKSG 660

Query: 661  RSGADFLGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKS 720
            RSGADFLGPLLPAVAEICSDFDP++++EPSLLKLFRNLWFYIALFGLAPPIQK Q  TK 
Sbjct: 661  RSGADFLGPLLPAVAEICSDFDPSVDVEPSLLKLFRNLWFYIALFGLAPPIQKIQHPTKP 720

Query: 721  VSSMLNSVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNAL 780
            VS+ LNSVGSMG IALQAV GPYMWNTQWSSAVQ IAQGTP LVVSSVKWLEDELELNAL
Sbjct: 721  VSTTLNSVGSMGTIALQAVGGPYMWNTQWSSAVQQIAQGTPSLVVSSVKWLEDELELNAL 780

Query: 781  HNPGSRQGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEIIRFSSN 840
            HNPGSR+GSGNEKAAV+QRAALSAALGGRVD+ +M+TISGVKATYLLAV+FLEIIRFSSN
Sbjct: 781  HNPGSRRGSGNEKAAVSQRAALSAALGGRVDVGSMNTISGVKATYLLAVAFLEIIRFSSN 840

Query: 841  GGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRISETG 900
            GGV+NGG++LNASRSAFSCVFEYLK+PNL+PAV QCL A+VH AFETAV WL+DRISETG
Sbjct: 841  GGVINGGTSLNASRSAFSCVFEYLKSPNLMPAVFQCLMAIVHRAFETAVLWLEDRISETG 900

Query: 901  NEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSM 960
             +AE R+STL AH CFLI+SMSQR+EH+RD+AVNLL QLRDKFPQV+WNSSC DSLLFSM
Sbjct: 901  MKAEYRESTLSAHACFLIKSMSQREEHIRDVAVNLLAQLRDKFPQVLWNSSCFDSLLFSM 960

Query: 961  HNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQRAQH 1020
            HND+PS VV DP+W+VTVRSLYQ +VREWIIKSLS+APCT+QGLLQ+KLCK NTWQRAQ 
Sbjct: 961  HNDSPSVVVNDPSWMVTVRSLYQKIVREWIIKSLSHAPCTTQGLLQDKLCKANTWQRAQP 1020

Query: 1021 TPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGTGMV 1080
            T DVISLLSEIRIGT KN+CWTGIQTANIPAVM AAAAASGA+ KLTEAFNLEVL TG+V
Sbjct: 1021 TTDVISLLSEIRIGTMKNECWTGIQTANIPAVMAAAAAASGANFKLTEAFNLEVLSTGIV 1080

Query: 1081 SATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNEDDSF 1140
            SATVKCNHAGEIAGMRRLYNSIGGFQ+G     G G G G QRLISGAFPQ+P  EDDSF
Sbjct: 1081 SATVKCNHAGEIAGMRRLYNSIGGFQSGTTTG-GFGIGIGLQRLISGAFPQQPLAEDDSF 1140

Query: 1141 NGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFARLI 1200
            NG+L+ KFV+ LQQFV+ +EKG  +DKL FRETCSQATALLLSNL S+SK+NIEGF++L+
Sbjct: 1141 NGMLLAKFVRMLQQFVNISEKGGDVDKLHFRETCSQATALLLSNLGSDSKSNIEGFSQLL 1200

Query: 1201 RLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEV 1260
            RLLCWCPAYISTPDA+ETGVFIWTWLVSAAPELGS VLAELVDAWLWTIDTKRGLFAS+V
Sbjct: 1201 RLLCWCPAYISTPDAMETGVFIWTWLVSAAPELGSLVLAELVDAWLWTIDTKRGLFASDV 1260

Query: 1261 KYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLLFGR 1320
            KYSGPAAKLRPHLSPGEPE QPEIDPVEQI+AHRIWLGFFIDRFEVVRHNSVEQ+LL GR
Sbjct: 1261 KYSGPAAKLRPHLSPGEPEGQPEIDPVEQIVAHRIWLGFFIDRFEVVRHNSVEQLLLLGR 1320

Query: 1321 LLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYRASL 1380
            +LQG+TK PWNFSRHPAA G+FFT+MLLGLKFCSCQSQGNL NFKTGL+LLEDRIYRASL
Sbjct: 1321 MLQGTTKLPWNFSRHPAAAGTFFTVMLLGLKFCSCQSQGNLHNFKTGLQLLEDRIYRASL 1380

Query: 1381 GWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVNSKD 1440
            GWFA+E EWYD+ ++NF+QSEAQSVS+F+HYLS++R +G Q D+K RGRENG ++V++  
Sbjct: 1381 GWFAYEAEWYDINNINFSQSEAQSVSVFVHYLSNERVDGGQSDSKVRGRENGSTLVDANH 1440

Query: 1441 QYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPKLSSEKWIE 1500
            QYHPVWG +ENYAVGREKR+QLLLMLCQHEADRLEVWAQP N K+ST SR K+SSEKWIE
Sbjct: 1441 QYHPVWGQMENYAVGREKRKQLLLMLCQHEADRLEVWAQPTNSKDSTSSRSKISSEKWIE 1500

Query: 1501 HVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDEN 1560
            + RTAFSVDPRIA S+ SRFPTN  L+ E+  LVQ +ILDIR IPEALPYFVTPKAVDEN
Sbjct: 1501 YARTAFSVDPRIALSLASRFPTNMFLKAEVTHLVQSNILDIRGIPEALPYFVTPKAVDEN 1560

Query: 1561 SELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALR 1620
            S LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQ+LR
Sbjct: 1561 SALLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQSLR 1620

Query: 1621 YDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVRQH 1680
            YD+ RLVEGYLLRAAQRSD+FAHILIWHLQGET VP+SGK+A SGKN SF  LLP+VR  
Sbjct: 1621 YDDARLVEGYLLRAAQRSDLFAHILIWHLQGETCVPESGKEAISGKNTSFYELLPLVRDR 1680

Query: 1681 IIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPT 1740
            IIDGF+PKAL+LF+REF+FFDKVTSISGVLFPLPKDERRAGIRRELEKIE++GEDLYLPT
Sbjct: 1681 IIDGFSPKALELFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEVDGEDLYLPT 1740

Query: 1741 ATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDVLA 1800
            A +KLVRGIQVDSGIPLQSAAKVPIMITFNVV+R GD ND+KPQACIFKVGDDCRQDVLA
Sbjct: 1741 APSKLVRGIQVDSGIPLQSAAKVPIMITFNVVDRYGDRNDVKPQACIFKVGDDCRQDVLA 1800

Query: 1801 LQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQ 1860
            LQVIALL DIF+AVGINLYL+PYGVLPTGP RGIIEVVPNTRSRSQMGETTDGGLYEIFQ
Sbjct: 1801 LQVIALLSDIFKAVGINLYLYPYGVLPTGPERGIIEVVPNTRSRSQMGETTDGGLYEIFQ 1860

Query: 1861 QDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFILE 1920
            QD+GPVG+P+FEAARENFIISSAGYAVASLLLQPKDRHNGNLL DN GRLVHIDFGFILE
Sbjct: 1861 QDHGPVGTPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILE 1920

Query: 1921 TSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTV 1980
            TSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR+MDGIINTV
Sbjct: 1921 TSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWHQFVSLCVKGYLAARRYMDGIINTV 1980

Query: 1981 LLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQ 2038
            LLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMI VCTDAYNKWTTAGYDLIQYLQ
Sbjct: 1981 LLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIHVCTDAYNKWTTAGYDLIQYLQ 2040

BLAST of MC04g0487 vs. TAIR 10
Match: AT1G49340.2 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 3023.4 bits (7837), Expect = 0.0e+00
Identity = 1529/2051 (74.55%), Postives = 1753/2051 (85.47%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            MEAL ELCD+IA+NP  FSEKL+WIC RCP +E  LA SP +SRS LNAVLAVAR++SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            P+S+  R KSVV EF+ +IP SF  SFWP S+ +  I+SFY +F++Y S A +LS EF T
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EISGFTSEVVLSAI---DDCSEGSP-ISRAFLVAMSKSFIPILPSDADKLVSCILDRFLI 180
            E++ FT EVV++AI      S+G P IS+AFLVA+S+ F  IL SD DKL++ +LD+F++
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  SEAAPGTPREQIRANS-----EASSSQGSPLSVSHHPLNGG--LSPGNENGQVSGSLSSG 240
            +  AP +P+EQ + NS     + SSSQGSP+S + +P       SPG+E       ++S 
Sbjct: 181  NR-APASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDE-------VASH 240

Query: 241  GSRITDDATSASSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESI 300
            GS +     S+ S  S +MNG S++WKSGVDQL        GG     RQQV  FEDESI
Sbjct: 241  GSNL-----SSKSSSSVVMNGGSIVWKSGVDQLSFGFSEGSGGANPVFRQQVASFEDESI 300

Query: 301  ENLEKQEIAFKLVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLK 360
            E+LEKQEIAF+L+THILD    D++L +Q+R + K+QLQ++  FLK +KRDW+EQG VLK
Sbjct: 301  ESLEKQEIAFRLITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQVLK 360

Query: 361  ARINTKLLVYQAAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRIC 420
             R+N KL VYQAAA++K+K++ SL+ DGK +K+L+ ET ALL+DAA+ACL SVWRK++ C
Sbjct: 361  TRVNAKLSVYQAAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMKAC 420

Query: 421  EELFGSLLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCC 480
            EELF SLL+G+A+IAVARGGQPLRVLLIRLKPLVL VCA  D     QGAM ES+ KT C
Sbjct: 421  EELFDSLLSGIAKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKTSC 480

Query: 481  EIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVN 540
             IIES W KDRAPVD FIMGLA+SIR+RND EEQ D+EKQ   +QLNVIRLLA + VAV 
Sbjct: 481  VIIESAWAKDRAPVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVAVK 540

Query: 541  KSEVVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLS 600
            K EV DMILPLFIESLEEGDASTP  LRLQLLDAVSR+A+LGF+KSYRET+VLMTRSYLS
Sbjct: 541  KPEVADMILPLFIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSYLS 600

Query: 601  KLSSIGSSESRTLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSA 660
            KLSS+GS ES+T APEA TERVETLPAGFL IASGL   KLRS+YRHRLLSLCSDVGL+A
Sbjct: 601  KLSSVGSVESKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGLAA 660

Query: 661  ESKSGRSGADFLGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQ 720
            ESKSG SG DFLGPLLPAVAEICSDFDPTM++EPSLLKLFRNLWFYIALFGLAPPI K+ 
Sbjct: 661  ESKSGGSGVDFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVKTP 720

Query: 721  L-QTKSVSSMLNSVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDE 780
                KS S+ +NSVGSM A ALQAV GPYMW+ QW+ AVQ IAQGTPPLVVSSVKWLEDE
Sbjct: 721  TPPLKSTSNSVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLEDE 780

Query: 781  LELNALHNPGSRQGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEI 840
            LELNALHNPGSR+G+GNEK A  QR ALS ALGGRVD+AAM+TISGVKATYLLAV+FLEI
Sbjct: 781  LELNALHNPGSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFLEI 840

Query: 841  IRFSSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDD 900
            IRF SNGG+LNG S+++ASRSAFSCVFEYLKTPNL PAVSQCLTA+VH AFETAVSWL+D
Sbjct: 841  IRFISNGGILNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWLED 900

Query: 901  RISETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLD 960
            RIS TG +A  R+ T +AH CFLI+SMSQRDEHVRDI+VNLLTQLRDKFPQV+W+SSCLD
Sbjct: 901  RISLTGKDARNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSCLD 960

Query: 961  SLLFSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNT 1020
            SLLFS+H++ PS VV DPAW   VRSLYQ VVREWII SLSYAPCTSQGLLQ+KLCK NT
Sbjct: 961  SLLFSVHDNTPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKANT 1020

Query: 1021 WQRAQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEV 1080
            WQRAQ T DV+SLLSEI+IGTGKN+ W+GI+TANIPAVM AAAAASGA+LK++EAFNLEV
Sbjct: 1021 WQRAQTTTDVVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNLEV 1080

Query: 1081 LGTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQ 1140
            LGTG+VSATVKCNHAGEIAGMRRLYNSIGGFQ+G    T  GFG G QRLISGAF Q PQ
Sbjct: 1081 LGTGVVSATVKCNHAGEIAGMRRLYNSIGGFQSG---STPSGFGGGLQRLISGAFSQAPQ 1140

Query: 1141 NEDDSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIE 1200
             EDDSFN +LI +FV+ LQQFV+ AEKG  ++K +FRETCSQATALLLSNL  ESKTN+E
Sbjct: 1141 PEDDSFNEMLIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTNVE 1200

Query: 1201 GFARLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRG 1260
            GF++L+RLLCWCPAYISTPDA+ETG+FIWTWLVSAAP+L S VLAELVDAW+WTIDTKRG
Sbjct: 1201 GFSQLLRLLCWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTKRG 1260

Query: 1261 LFASEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ 1320
            LFAS+V+YSGPAAKLRPHLSPGEPE  PE DPV+QI+AHR+WLGF IDRFEVVRHNS EQ
Sbjct: 1261 LFASDVRYSGPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSAEQ 1320

Query: 1321 VLLFGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDR 1380
            +LL GR+LQ ST   W F+RHPAA G+FF+LMLLGLKFCSCQ+QGN+Q F++GL+LLEDR
Sbjct: 1321 LLLLGRMLQRSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLEDR 1380

Query: 1381 IYRASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGIS 1440
            IYR SLGWFAH+PEWYDV   NF  SEA SVS+F+H+LS++     Q D+K + RE+G +
Sbjct: 1381 IYRTSLGWFAHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESG-N 1440

Query: 1441 VVNSKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPKLS 1500
            +++  DQYHPVWG ++NY +G+EKR+QLLLMLCQHEADRL+VWAQP + K+S  SR K+S
Sbjct: 1441 LIDVTDQYHPVWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLKIS 1500

Query: 1501 SEKWIEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTP 1560
            SEKW E+ +TAFSVDPRIA S+ SRFP NAS++ E+ QLVQ +I+D+R+IPEALPYFVTP
Sbjct: 1501 SEKWTEYAKTAFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFVTP 1560

Query: 1561 KAVDENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQ 1620
            K V+ENS LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE+VTFFMPQ
Sbjct: 1561 KNVEENSVLLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFMPQ 1620

Query: 1621 LVQALRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALL 1680
            LVQ+LRYD+GRLVEGYLLRA QRSDIFAHILIWHLQGE  V ++ KD +  KN +F  +L
Sbjct: 1621 LVQSLRYDDGRLVEGYLLRATQRSDIFAHILIWHLQGE-DVQETPKDGSIDKNAAFQEIL 1680

Query: 1681 PVVRQHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGE 1740
            P VRQHIIDGF+P ALD+F REF+FFDKVTSISGVLFPLPK+ERRAGIRRELEKIEM+G+
Sbjct: 1681 PQVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIEMQGD 1740

Query: 1741 DLYLPTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDC 1800
            DLYLPTA NKLVRGI+VDSGIPLQSAAKVPIMITFNV++RDGD +D+KPQACIFKVGDDC
Sbjct: 1741 DLYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKVGDDC 1800

Query: 1801 RQDVLALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGG 1860
            RQDVLALQVI+LLRDIFQA G+NLYLFPYGVLPTG  RGIIEVVPNTRSRSQMGETTDGG
Sbjct: 1801 RQDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGETTDGG 1860

Query: 1861 LYEIFQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHID 1920
            LYEIFQQDYGPVGS  FE ARENF+ISSAGYAVASLLLQPKDRHNGNLL D+ GRLVHID
Sbjct: 1861 LYEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHID 1920

Query: 1921 FGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMD 1980
            FGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR MD
Sbjct: 1921 FGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMD 1980

Query: 1981 GIINTVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYD 2039
            GII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FMI VCTDAYNKWTTAGYD
Sbjct: 1981 GIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYD 2028

BLAST of MC04g0487 vs. TAIR 10
Match: AT1G49340.1 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 3023.4 bits (7837), Expect = 0.0e+00
Identity = 1529/2051 (74.55%), Postives = 1753/2051 (85.47%), Query Frame = 0

Query: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
            MEAL ELCD+IA+NP  FSEKL+WIC RCP +E  LA SP +SRS LNAVLAVAR++SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120
            P+S+  R KSVV EF+ +IP SF  SFWP S+ +  I+SFY +F++Y S A +LS EF T
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EISGFTSEVVLSAI---DDCSEGSP-ISRAFLVAMSKSFIPILPSDADKLVSCILDRFLI 180
            E++ FT EVV++AI      S+G P IS+AFLVA+S+ F  IL SD DKL++ +LD+F++
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  SEAAPGTPREQIRANS-----EASSSQGSPLSVSHHPLNGG--LSPGNENGQVSGSLSSG 240
            +  AP +P+EQ + NS     + SSSQGSP+S + +P       SPG+E       ++S 
Sbjct: 181  NR-APASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDE-------VASH 240

Query: 241  GSRITDDATSASSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESI 300
            GS +     S+ S  S +MNG S++WKSGVDQL        GG     RQQV  FEDESI
Sbjct: 241  GSNL-----SSKSSSSVVMNGGSIVWKSGVDQLSFGFSEGSGGANPVFRQQVASFEDESI 300

Query: 301  ENLEKQEIAFKLVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLK 360
            E+LEKQEIAF+L+THILD    D++L +Q+R + K+QLQ++  FLK +KRDW+EQG VLK
Sbjct: 301  ESLEKQEIAFRLITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQVLK 360

Query: 361  ARINTKLLVYQAAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRIC 420
             R+N KL VYQAAA++K+K++ SL+ DGK +K+L+ ET ALL+DAA+ACL SVWRK++ C
Sbjct: 361  TRVNAKLSVYQAAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMKAC 420

Query: 421  EELFGSLLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCC 480
            EELF SLL+G+A+IAVARGGQPLRVLLIRLKPLVL VCA  D     QGAM ES+ KT C
Sbjct: 421  EELFDSLLSGIAKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKTSC 480

Query: 481  EIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVN 540
             IIES W KDRAPVD FIMGLA+SIR+RND EEQ D+EKQ   +QLNVIRLLA + VAV 
Sbjct: 481  VIIESAWAKDRAPVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVAVK 540

Query: 541  KSEVVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLS 600
            K EV DMILPLFIESLEEGDASTP  LRLQLLDAVSR+A+LGF+KSYRET+VLMTRSYLS
Sbjct: 541  KPEVADMILPLFIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSYLS 600

Query: 601  KLSSIGSSESRTLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSA 660
            KLSS+GS ES+T APEA TERVETLPAGFL IASGL   KLRS+YRHRLLSLCSDVGL+A
Sbjct: 601  KLSSVGSVESKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGLAA 660

Query: 661  ESKSGRSGADFLGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQ 720
            ESKSG SG DFLGPLLPAVAEICSDFDPTM++EPSLLKLFRNLWFYIALFGLAPPI K+ 
Sbjct: 661  ESKSGGSGVDFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVKTP 720

Query: 721  L-QTKSVSSMLNSVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDE 780
                KS S+ +NSVGSM A ALQAV GPYMW+ QW+ AVQ IAQGTPPLVVSSVKWLEDE
Sbjct: 721  TPPLKSTSNSVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLEDE 780

Query: 781  LELNALHNPGSRQGSGNEKAAVAQRAALSAALGGRVDIAAMSTISGVKATYLLAVSFLEI 840
            LELNALHNPGSR+G+GNEK A  QR ALS ALGGRVD+AAM+TISGVKATYLLAV+FLEI
Sbjct: 781  LELNALHNPGSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFLEI 840

Query: 841  IRFSSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDD 900
            IRF SNGG+LNG S+++ASRSAFSCVFEYLKTPNL PAVSQCLTA+VH AFETAVSWL+D
Sbjct: 841  IRFISNGGILNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWLED 900

Query: 901  RISETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLD 960
            RIS TG +A  R+ T +AH CFLI+SMSQRDEHVRDI+VNLLTQLRDKFPQV+W+SSCLD
Sbjct: 901  RISLTGKDARNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSCLD 960

Query: 961  SLLFSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNT 1020
            SLLFS+H++ PS VV DPAW   VRSLYQ VVREWII SLSYAPCTSQGLLQ+KLCK NT
Sbjct: 961  SLLFSVHDNTPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKANT 1020

Query: 1021 WQRAQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEV 1080
            WQRAQ T DV+SLLSEI+IGTGKN+ W+GI+TANIPAVM AAAAASGA+LK++EAFNLEV
Sbjct: 1021 WQRAQTTTDVVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNLEV 1080

Query: 1081 LGTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQ 1140
            LGTG+VSATVKCNHAGEIAGMRRLYNSIGGFQ+G    T  GFG G QRLISGAF Q PQ
Sbjct: 1081 LGTGVVSATVKCNHAGEIAGMRRLYNSIGGFQSG---STPSGFGGGLQRLISGAFSQAPQ 1140

Query: 1141 NEDDSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIE 1200
             EDDSFN +LI +FV+ LQQFV+ AEKG  ++K +FRETCSQATALLLSNL  ESKTN+E
Sbjct: 1141 PEDDSFNEMLIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTNVE 1200

Query: 1201 GFARLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRG 1260
            GF++L+RLLCWCPAYISTPDA+ETG+FIWTWLVSAAP+L S VLAELVDAW+WTIDTKRG
Sbjct: 1201 GFSQLLRLLCWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTKRG 1260

Query: 1261 LFASEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ 1320
            LFAS+V+YSGPAAKLRPHLSPGEPE  PE DPV+QI+AHR+WLGF IDRFEVVRHNS EQ
Sbjct: 1261 LFASDVRYSGPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSAEQ 1320

Query: 1321 VLLFGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDR 1380
            +LL GR+LQ ST   W F+RHPAA G+FF+LMLLGLKFCSCQ+QGN+Q F++GL+LLEDR
Sbjct: 1321 LLLLGRMLQRSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLEDR 1380

Query: 1381 IYRASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGIS 1440
            IYR SLGWFAH+PEWYDV   NF  SEA SVS+F+H+LS++     Q D+K + RE+G +
Sbjct: 1381 IYRTSLGWFAHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESG-N 1440

Query: 1441 VVNSKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPKLS 1500
            +++  DQYHPVWG ++NY +G+EKR+QLLLMLCQHEADRL+VWAQP + K+S  SR K+S
Sbjct: 1441 LIDVTDQYHPVWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLKIS 1500

Query: 1501 SEKWIEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTP 1560
            SEKW E+ +TAFSVDPRIA S+ SRFP NAS++ E+ QLVQ +I+D+R+IPEALPYFVTP
Sbjct: 1501 SEKWTEYAKTAFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFVTP 1560

Query: 1561 KAVDENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQ 1620
            K V+ENS LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE+VTFFMPQ
Sbjct: 1561 KNVEENSVLLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFMPQ 1620

Query: 1621 LVQALRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALL 1680
            LVQ+LRYD+GRLVEGYLLRA QRSDIFAHILIWHLQGE  V ++ KD +  KN +F  +L
Sbjct: 1621 LVQSLRYDDGRLVEGYLLRATQRSDIFAHILIWHLQGE-DVQETPKDGSIDKNAAFQEIL 1680

Query: 1681 PVVRQHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGE 1740
            P VRQHIIDGF+P ALD+F REF+FFDKVTSISGVLFPLPK+ERRAGIRRELEKIEM+G+
Sbjct: 1681 PQVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIEMQGD 1740

Query: 1741 DLYLPTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDC 1800
            DLYLPTA NKLVRGI+VDSGIPLQSAAKVPIMITFNV++RDGD +D+KPQACIFKVGDDC
Sbjct: 1741 DLYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKVGDDC 1800

Query: 1801 RQDVLALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGG 1860
            RQDVLALQVI+LLRDIFQA G+NLYLFPYGVLPTG  RGIIEVVPNTRSRSQMGETTDGG
Sbjct: 1801 RQDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGETTDGG 1860

Query: 1861 LYEIFQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHID 1920
            LYEIFQQDYGPVGS  FE ARENF+ISSAGYAVASLLLQPKDRHNGNLL D+ GRLVHID
Sbjct: 1861 LYEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHID 1920

Query: 1921 FGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMD 1980
            FGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR MD
Sbjct: 1921 FGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMD 1980

Query: 1981 GIINTVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYD 2039
            GII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FMI VCTDAYNKWTTAGYD
Sbjct: 1981 GIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYD 2028

BLAST of MC04g0487 vs. TAIR 10
Match: AT1G51040.1 (Protein kinase superfamily protein )

HSP 1 Score: 708.4 bits (1827), Expect = 1.7e-203
Identity = 351/420 (83.57%), Postives = 376/420 (89.52%), Query Frame = 0

Query: 1619 LVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVRQHIIDGF 1678
            +VEGYLLRAAQRSDIFAH LIWHLQGE SV +  KD    KN SF  +L  VRQHI+DGF
Sbjct: 115  MVEGYLLRAAQRSDIFAHTLIWHLQGE-SVEEIVKDGAFDKNASFQEILSDVRQHIVDGF 174

Query: 1679 TPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKL 1738
            TPKAL+ F REF+FF+KVTSISG L PLPK+ER AGIRRELEKI+M+GEDLYLPTA NKL
Sbjct: 175  TPKALNFFSREFDFFEKVTSISGALLPLPKEERVAGIRRELEKIKMQGEDLYLPTAPNKL 234

Query: 1739 VRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDVLALQVIA 1798
            VR IQVDSGIPLQSAAKVPIMITFNVV+ DGD ND+KPQACIFKVGDDCRQDVLALQVI+
Sbjct: 235  VRVIQVDSGIPLQSAAKVPIMITFNVVDLDGDHNDVKPQACIFKVGDDCRQDVLALQVIS 294

Query: 1799 LLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGP 1858
            LL DIFQAVG+NLYLFPYGVLPT        VVPNTRSRSQMGETTDGGLYEIFQQ+YG 
Sbjct: 295  LLGDIFQAVGLNLYLFPYGVLPT--------VVPNTRSRSQMGETTDGGLYEIFQQNYGL 354

Query: 1859 VGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFILETSPGG 1918
            VGS  FE AR NF+ISSAGYAVASLLLQPKDRHNGNLL D+ GRLVHIDFGFILETSPGG
Sbjct: 355  VGSTTFETARANFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGG 414

Query: 1919 NMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLD 1978
            NMRFE+AHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR+MD II+TV +ML+
Sbjct: 415  NMRFENAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRYMDEIISTVQMMLE 474

Query: 1979 SGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2038
            SGLPCFSRGDPIGNLRKRFHPEMSEREAA FMI VCTDAYNKWTTAGYDLIQYLQQG+EK
Sbjct: 475  SGLPCFSRGDPIGNLRKRFHPEMSEREAALFMINVCTDAYNKWTTAGYDLIQYLQQGVEK 525

HSP 2 Score: 117.9 bits (294), Expect = 9.8e-26
Identity = 56/117 (47.86%), Postives = 78/117 (66.67%), Query Frame = 0

Query: 1   MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60
           ME L ELCD+I QNP  F +KL WIC +CP S++  A SP  S+S LNA+LAV R+LSK 
Sbjct: 1   MERLNELCDIIEQNPKQFLDKLPWICQQCPQSKLLRAESPRFSQSHLNAILAVTRILSKI 60

Query: 61  PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTE 118
            D+     K VV++F++++P SF+ SFWP S   ++I++FY  F+ Y S   +L  E
Sbjct: 61  VDTTDENAKFVVLDFLQTVPKSFHRSFWPYSLSLESISAFYCSFLGYVSCLSQLMVE 117

BLAST of MC04g0487 vs. TAIR 10
Match: AT5G64070.1 (phosphatidylinositol 4-OH kinase beta1 )

HSP 1 Score: 211.5 bits (537), Expect = 6.5e-54
Identity = 113/266 (42.48%), Postives = 165/266 (62.03%), Query Frame = 0

Query: 1777 QACIFKVGDDCRQDVLALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRS 1836
            ++ I K GDDCRQ+ LA+Q+I+   DIFQ  G+ L+L PY VL T     +IE +P+T S
Sbjct: 859  RSIIVKSGDDCRQEHLAVQLISHFYDIFQEAGLPLWLRPYEVLVTSSYTALIETIPDTAS 918

Query: 1837 ----RSQMGETTDGGLYEIFQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHN 1896
                +S+    T   L + F   Y    SP+F+ A+ NF+ S AGY++   LLQ KDRHN
Sbjct: 919  IHSIKSRYPNIT--SLRDFFVAKY-KENSPSFKLAQRNFVESMAGYSLVCYLLQVKDRHN 978

Query: 1897 GNLLIDNTGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLD-PSGVMKSETWNLF 1956
            GNLL+D  G ++HIDFGF+L  SPGG + FESA FKL+ E+ +++D  +  + SE ++ F
Sbjct: 979  GNLLLDEEGHIIHIDFGFMLSNSPGG-VNFESAPFKLTRELLEVMDSDADGVPSEFFDYF 1038

Query: 1957 VSLCVKGYLAARRHMDGIINTVLLMLDSGLPCFSRGD-PIGNLRKRFHPEMSEREAANFM 2016
              LC++G+L  R+H + II  V ++ DSG PCF  G   I NLRKRFH  ++E +  + +
Sbjct: 1039 KVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLV 1098

Query: 2017 IRVCTDAYNKWTTAGYDLIQYLQQGI 2037
            + + + + + W T  YD  Q +  GI
Sbjct: 1099 LSLISSSLDAWRTRQYDYYQRVLNGI 1120

BLAST of MC04g0487 vs. TAIR 10
Match: AT5G09350.1 (phosphatidylinositol 4-OH kinase beta2 )

HSP 1 Score: 210.3 bits (534), Expect = 1.5e-53
Identity = 112/266 (42.11%), Postives = 164/266 (61.65%), Query Frame = 0

Query: 1777 QACIFKVGDDCRQDVLALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRS 1836
            ++ I K GDDCRQ+ LA+Q+I+   DIFQ  G+ L+L PY VL T     +IE +P+T S
Sbjct: 854  RSIIVKSGDDCRQEHLAVQLISHFFDIFQEAGLPLWLRPYEVLVTSSYTALIETIPDTAS 913

Query: 1837 ----RSQMGETTDGGLYEIFQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHN 1896
                +S+    T   L + F   +    SP+F+ A+ NF+ S AGY++   LLQ KDRHN
Sbjct: 914  IHSIKSRYPNIT--SLRDFFDAKF-KENSPSFKLAQRNFVESMAGYSLVCYLLQIKDRHN 973

Query: 1897 GNLLIDNTGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGV-MKSETWNLF 1956
            GNLL+D  G ++HIDFGF+L  SPGG + FESA FKL+ E+ +++D     + SE ++ F
Sbjct: 974  GNLLMDEEGHIIHIDFGFMLSNSPGG-VNFESAPFKLTRELLEVMDSDAEGLPSEFFDYF 1033

Query: 1957 VSLCVKGYLAARRHMDGIINTVLLMLDSGLPCFSRGD-PIGNLRKRFHPEMSEREAANFM 2016
              LC++G+L  R+H + II  V ++ DSG PCF  G   I NLRKRFH  ++E +  + +
Sbjct: 1034 KVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLV 1093

Query: 2017 IRVCTDAYNKWTTAGYDLIQYLQQGI 2037
            + + + + + W T  YD  Q +  GI
Sbjct: 1094 LSLISSSLDAWRTRQYDYYQRVLNGI 1115