MC04g0453 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0453
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Cysteine Protease
Location	MC04: 3643466 .. 3643573 (-)
RNA-Seq Expression	MC04g0453
Synteny	MC04g0453

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATCTTGCGGGTCCTGTACAACTGGTGCTCTAGAAGGTGCCAATTTTCTTTCAAAAGGCGAACTTGTTAGCATAAGCGAACAGCAGCCGGTAGATTGCGATCACGAG

mRNA sequence

GGATCTTGCGGGTCCTGTACAACTGGTGCTCTAGAAGGTGCCAATTTTCTTTCAAAAGGCGAACTTGTTAGCATAAGCGAACAGCAGCCGGTAGATTGCGATCACGAG

Coding sequence (CDS)

GGATCTTGCGGGTCCTGTACAACTGGTGCTCTAGAAGGTGCCAATTTTCTTTCAAAAGGCGAACTTGTTAGCATAAGCGAACAGCAGCCGGTAGATTGCGATCACGAG

Protein sequence

GSCGSCTTGALEGANFLSKGELVSISEQQPVDCDHE

Homology

[Close]

BLAST of MC04g0453 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 4.6e-09
Identity = 30/40 (75.00%), Postives = 33/40 (82.50%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 37
           GSCGSC     TGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 154 GSCGSCWSFSATGALEGANFLATGKLVSLSEQQLVDCDHE 193

[Close]

BLAST of MC04g0453 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 4.6e-09
Identity = 30/40 (75.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 37
           GSCGSC    TTGALEGA+FL+ G+LVS+SEQQ VDCDHE
Sbjct: 151 GSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQLVDCDHE 190

[Close]

BLAST of MC04g0453 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.9e-08
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDH 36
           GSCGSC    TTGALEGA++L+ G+LVS+SEQQ VDCDH
Sbjct: 151 GSCGSCWAFSTTGALEGAHYLATGKLVSLSEQQLVDCDH 189

[Close]

BLAST of MC04g0453 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 9.6e-07
Identity = 27/40 (67.50%), Postives = 30/40 (75.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 37
           G CGSC      GALEGA+FL+  ELVS+SEQQ VDCDHE
Sbjct: 159 GMCGSCWSFSAIGALEGAHFLATKELVSLSEQQLVDCDHE 198

[Close]

BLAST of MC04g0453 vs. ExPASy Swiss-Prot
Match: Q8VYS0 (Probable cysteine protease RD19D OS=Arabidopsis thaliana OX=3702 GN=RD19D PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.8e-06
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCD 35
           G+CGSC    TTGA EGA+F+S G+L+S+SEQQ VDCD
Sbjct: 156 GACGSCWAFSTTGAAEGAHFVSTGKLLSLSEQQLVDCD 193

[Close]

BLAST of MC04g0453 vs. NCBI nr
Match: MBL2417152.1 (hypothetical protein [Klebsiella pneumoniae])

HSP 1 Score: 63.9 bits (154), Expect = 5.76e-12
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1  GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 36
          GSCGSC    TTGALEGANFLS G+LVS+SEQQ VDCDHE
Sbjct: 2  GSCGSCWSFSTTGALEGANFLSTGKLVSLSEQQLVDCDHE 41

[Close]

BLAST of MC04g0453 vs. NCBI nr
Match: KAA8523797.1 (hypothetical protein F0562_010220 [Nyssa sinensis])

HSP 1 Score: 62.8 bits (151), Expect = 1.53e-11
Identity = 31/40 (77.50%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1  GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 36
          GSCGSC    TTGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 31 GSCGSCWSFSTTGALEGANFLATGKLVSLSEQQLVDCDHE 70

[Close]

BLAST of MC04g0453 vs. NCBI nr
Match: TYI83701.1 (hypothetical protein E1A91_D05G311700v1, partial [Gossypium mustelinum])

HSP 1 Score: 62.8 bits (151), Expect = 4.97e-11
Identity = 31/40 (77.50%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 36
           GSCGSC    TTGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 91  GSCGSCWSFITTGALEGANFLATGKLVSLSEQQLVDCDHE 130

[Close]

BLAST of MC04g0453 vs. NCBI nr
Match: TYH73316.1 (hypothetical protein ES332_D05G320600v1, partial [Gossypium tomentosum])

HSP 1 Score: 62.8 bits (151), Expect = 5.54e-11
Identity = 31/40 (77.50%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 36
           GSCGSC    TTGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 96  GSCGSCWSFITTGALEGANFLATGKLVSLSEQQLVDCDHE 135

[Close]

BLAST of MC04g0453 vs. NCBI nr
Match: KJB60399.1 (hypothetical protein B456_009G303700, partial [Gossypium raimondii])

HSP 1 Score: 62.8 bits (151), Expect = 5.54e-11
Identity = 31/40 (77.50%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 36
           GSCGSC    TTGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 96  GSCGSCWSFITTGALEGANFLATGKLVSLSEQQLVDCDHE 135

[Close]

BLAST of MC04g0453 vs. ExPASy TrEMBL
Match: A0A5J5A3A1 (Pept_C1 domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_010220 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 7.41e-12
Identity = 31/40 (77.50%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1  GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 36
          GSCGSC    TTGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 31 GSCGSCWSFSTTGALEGANFLATGKLVSLSEQQLVDCDHE 70

[Close]

BLAST of MC04g0453 vs. ExPASy TrEMBL
Match: A0A5K1AX81 (Pept_C1 domain-containing protein (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS12654 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.65e-11
Identity = 30/40 (75.00%), Postives = 33/40 (82.50%), Query Frame = 0


Query: 1  GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 36
          GSCGSC     TGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 1  GSCGSCWSFSATGALEGANFLATGKLVSLSEQQLVDCDHE 40

[Close]

BLAST of MC04g0453 vs. ExPASy TrEMBL
Match: A0A5K1GZ44 (Pept_C1 domain-containing protein (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS28103 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.65e-11
Identity = 30/40 (75.00%), Postives = 33/40 (82.50%), Query Frame = 0


Query: 1  GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 36
          GSCGSC     TGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 1  GSCGSCWTFSATGALEGANFLATGKLVSLSEQQLVDCDHE 40

[Close]

BLAST of MC04g0453 vs. ExPASy TrEMBL
Match: A0A5D2V310 (Pept_C1 domain-containing protein (Fragment) OS=Gossypium mustelinum OX=34275 GN=E1A91_D05G311700v1 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.41e-11
Identity = 31/40 (77.50%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 36
           GSCGSC    TTGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 91  GSCGSCWSFITTGALEGANFLATGKLVSLSEQQLVDCDHE 130

[Close]

BLAST of MC04g0453 vs. ExPASy TrEMBL
Match: A0A5D2L2M3 (Pept_C1 domain-containing protein (Fragment) OS=Gossypium tomentosum OX=34277 GN=ES332_D05G320600v1 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.68e-11
Identity = 31/40 (77.50%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 36
           GSCGSC    TTGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 96  GSCGSCWSFITTGALEGANFLATGKLVSLSEQQLVDCDHE 135

[Close]

BLAST of MC04g0453 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 60.5 bits (145), Expect = 3.3e-10
Identity = 30/40 (75.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 37
           GSCGSC    TTGALEGA+FL+ G+LVS+SEQQ VDCDHE
Sbjct: 151 GSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQLVDCDHE 190

[Close]

BLAST of MC04g0453 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 60.5 bits (145), Expect = 3.3e-10
Identity = 30/40 (75.00%), Postives = 33/40 (82.50%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 37
           GSCGSC     TGALEGANFL+ G+LVS+SEQQ VDCDHE
Sbjct: 154 GSCGSCWSFSATGALEGANFLATGKLVSLSEQQLVDCDHE 193

[Close]

BLAST of MC04g0453 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 52.8 bits (125), Expect = 6.8e-08
Identity = 27/40 (67.50%), Postives = 30/40 (75.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCDHE 37
           G CGSC      GALEGA+FL+  ELVS+SEQQ VDCDHE
Sbjct: 159 GMCGSCWSFSAIGALEGAHFLATKELVSLSEQQLVDCDHE 198

[Close]

BLAST of MC04g0453 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 51.2 bits (121), Expect = 2.0e-07
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCD 35
           G+CGSC    TTGA EGA+F+S G+L+S+SEQQ VDCD
Sbjct: 156 GACGSCWAFSTTGAAEGAHFVSTGKLLSLSEQQLVDCD 193

[Close]

BLAST of MC04g0453 vs. TAIR 10
Match: AT3G43960.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 46.6 bits (109), Expect = 4.9e-06
Identity = 22/38 (57.89%), Postives = 28/38 (73.68%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLSKGELVSISEQQPVDCD 35
           G CGSC     TGA+EG N ++ GELVS+SEQ+ +DCD
Sbjct: 147 GECGSCWAFAATGAVEGINQITTGELVSLSEQELIDCD 184

The following BLAST results are available for this feature:

BLAST of MC04g0453 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43296	4.6e-09	75.00	Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1	[more]
P43295	4.6e-09	75.00	Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S...	[more]
P25804	3.9e-08	71.79	Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
Q9SUL1	9.6e-07	67.50	Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S...	[more]
Q8VYS0	2.8e-06	65.79	Probable cysteine protease RD19D OS=Arabidopsis thaliana OX=3702 GN=RD19D PE=2 S...	[more]

BLAST of MC04g0453 vs. NCBI nr


Match Name	E-value	Identity	Description
MBL2417152.1	5.76e-12	80.00	hypothetical protein [Klebsiella pneumoniae]	[more]
KAA8523797.1	1.53e-11	77.50	hypothetical protein F0562_010220 [Nyssa sinensis]	[more]
TYI83701.1	4.97e-11	77.50	hypothetical protein E1A91_D05G311700v1, partial [Gossypium mustelinum]	[more]
TYH73316.1	5.54e-11	77.50	hypothetical protein ES332_D05G320600v1, partial [Gossypium tomentosum]	[more]
KJB60399.1	5.54e-11	77.50	hypothetical protein B456_009G303700, partial [Gossypium raimondii]	[more]

BLAST of MC04g0453 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5J5A3A1	7.41e-12	77.50	Pept_C1 domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_010220 PE...	[more]
A0A5K1AX81	1.65e-11	75.00	Pept_C1 domain-containing protein (Fragment) OS=Nymphaea colorata OX=210225 GN=N...	[more]
A0A5K1GZ44	1.65e-11	75.00	Pept_C1 domain-containing protein (Fragment) OS=Nymphaea colorata OX=210225 GN=N...	[more]
A0A5D2V310	2.41e-11	77.50	Pept_C1 domain-containing protein (Fragment) OS=Gossypium mustelinum OX=34275 GN...	[more]
A0A5D2L2M3	2.68e-11	77.50	Pept_C1 domain-containing protein (Fragment) OS=Gossypium tomentosum OX=34277 GN...	[more]

BLAST of MC04g0453 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21430.1	3.3e-10	75.00	Papain family cysteine protease	[more]
AT4G39090.1	3.3e-10	75.00	Papain family cysteine protease	[more]
AT4G16190.1	6.8e-08	67.50	Papain family cysteine protease	[more]
AT3G54940.2	2.0e-07	65.79	Papain family cysteine protease	[more]
AT3G43960.1	4.9e-06	57.89	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 1..36 e-value: 8.7E-8 score: 34.0
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 1..35 e-value: 1.9E-6 score: 28.1
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 1..35

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0453.1	MC04g0453.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008234	cysteine-type peptidase activity