MC04g0422 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0422
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	DUF2431 domain-containing protein
Location	MC04: 3348608 .. 3349547 (-)
RNA-Seq Expression	MC04g0422
Synteny	MC04g0422

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAATGCATTACAGCAGCAACCAGAAGATACTGCTTGCGGGAGAGGGAGATTTCTCCTTTTCAGCTTGCTTAGCCACAGCCTTTGGCTCTGCTACTAAAATATTTGCCACTTCTCTTGACTCCAAAGGTATTAAGAATGACCATATAATGGTTGCAAATTCATGTTCTCACTCTCTTTTTCTTGGGCATAACTGTTGGTCTATGTTTTTAAATGTGTAAAATAAAAAGAGTCGCTATTGAGGAAGTATGCACAAGCTGAGAGACACTTGAAACACTTGGAGGTCCTCGGTTGTACTGTTGTTCATGAAGTGGACGCAGGAAGCATGAGCCAACACCTCCTTCTCGGACTCGAGTCATTCGATCGGATTGCTTCAACTTCCCACATGCTGGTTTTGACCTCCGAGAGCGAGACGATGTTCAAATCTGTGAGTGAATGATTAACTTAAGCATCTGGTTTTAATGATAGTTTAACATGGTATCAGAGCACGAACCATCTTATTTGTTGGCCTTGTGCTGGTTTCTTAGCTCATAAGCAAGGGGAGGGTTAGGAATTGGAATAAAAGATTAAACTAAAAGATATGCAAAACTCACTCTAAATTGATTCTAATCAATCAGCTTAAAGCTCCTGAGTTTGATGTTAGTTTAATAGTGTGGTTTTGTATTGGGATGTGATACTCACAAAGTTTTAGTGGGAAAGTTTTTGAGGAATGCAAAGGAAATGCTGGCTGAAGATGGAGAAATTCACATTACCCACAAGACCTTGTATCCTTTTAGTGAATGGAACATTGAGAAACTAGCAGCAAGGGTGGGTCTCATTCGGATGGAGAGGGAATATTTTCGTAAAAGGGACTATCCAGGTTACGAAAACAAGAGAGGCGATGGGCCCGATAGCGATGCCACTTTCCACATTGGATCATGCAGCACATTCAAGTTTGCAAAG

mRNA sequence

ATAATGCATTACAGCAGCAACCAGAAGATACTGCTTGCGGGAGAGGGAGATTTCTCCTTTTCAGCTTGCTTAGCCACAGCCTTTGGCTCTGCTACTAAAATATTTGCCACTTCTCTTGACTCCAAAGAGTCGCTATTGAGGAAGTATGCACAAGCTGAGAGACACTTGAAACACTTGGAGGTCCTCGGTTGTACTGTTGTTCATGAAGTGGACGCAGGAAGCATGAGCCAACACCTCCTTCTCGGACTCGAGTCATTCGATCGGATTTTCAACTTCCCACATGCTGGTTTTGACCTCCGAGAGCGAGACGATGTTCAAATCTTTTTAGTGGGAAAGTTTTTGAGGAATGCAAAGGAAATGCTGGCTGAAGATGGAGAAATTCACATTACCCACAAGACCTTGTATCCTTTTAGTGAATGGAACATTGAGAAACTAGCAGCAAGGGTGGGTCTCATTCGGATGGAGAGGGAATATTTTCGTAAAAGGGACTATCCAGGTTACGAAAACAAGAGAGGCGATGGGCCCGATAGCGATGCCACTTTCCACATTGGATCATGCAGCACATTCAAGTTTGCAAAG

Coding sequence (CDS)

ATAATGCATTACAGCAGCAACCAGAAGATACTGCTTGCGGGAGAGGGAGATTTCTCCTTTTCAGCTTGCTTAGCCACAGCCTTTGGCTCTGCTACTAAAATATTTGCCACTTCTCTTGACTCCAAAGAGTCGCTATTGAGGAAGTATGCACAAGCTGAGAGACACTTGAAACACTTGGAGGTCCTCGGTTGTACTGTTGTTCATGAAGTGGACGCAGGAAGCATGAGCCAACACCTCCTTCTCGGACTCGAGTCATTCGATCGGATTTTCAACTTCCCACATGCTGGTTTTGACCTCCGAGAGCGAGACGATGTTCAAATCTTTTTAGTGGGAAAGTTTTTGAGGAATGCAAAGGAAATGCTGGCTGAAGATGGAGAAATTCACATTACCCACAAGACCTTGTATCCTTTTAGTGAATGGAACATTGAGAAACTAGCAGCAAGGGTGGGTCTCATTCGGATGGAGAGGGAATATTTTCGTAAAAGGGACTATCCAGGTTACGAAAACAAGAGAGGCGATGGGCCCGATAGCGATGCCACTTTCCACATTGGATCATGCAGCACATTCAAGTTTGCAAAG

Protein sequence

IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLEVLGCTVVHEVDAGSMSQHLLLGLESFDRIFNFPHAGFDLRERDDVQIFLVGKFLRNAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGPDSDATFHIGSCSTFKFAK

Homology

[Close]

BLAST of MC04g0422 vs. ExPASy Swiss-Prot
Match: F4I1X0 (Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX=3702 GN=HIPP41 PE=3 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.9e-49
Identity = 104/196 (53.06%), Postives = 131/196 (66.84%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           + HYSSN +ILL GEGDFSFS  LAT FGSA+ I A+SLDS + ++RKY +A  +LK L+
Sbjct: 21  VKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKYKKARSNLKTLK 80

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDR-IFNFPHAGFDLRERDDVQI----FLVGKFLR 120
            LG  ++H VDA ++  H  L    FDR IFNFPHAGF  RE D   I     LV  F  
Sbjct: 81  RLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKHRELVFGFFN 140

Query: 121 NAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
            A  +L  +GE+H++HK   PFSEWN+E+LA+R  L+ ++R  F K +YPGYENKRGDG 
Sbjct: 141 GASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYPGYENKRGDGR 200

Query: 181 DSDATFHIGSCSTFKF 192
             D  F +G CSTFKF
Sbjct: 201 RCDQPFLLGECSTFKF 216

[Close]

BLAST of MC04g0422 vs. ExPASy Swiss-Prot
Match: P0C8L4 (Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 1.8e-46
Identity = 108/201 (53.73%), Postives = 127/201 (63.18%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           I HYSS  KILL GEG+FSFS CLA+AFGSA  I ATSLDS++ L  KY  A  ++  L+
Sbjct: 8   IRHYSSTHKILLVGEGNFSFSLCLASAFGSAMNITATSLDSEDELSIKYMDAVDNINILK 67

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRI-FNFPHAG--FDLRERDDVQI----FLVGKF 120
             GC + HEVD  +MS    L L+ +DRI FNFPHAG  F  RE     I     LV  F
Sbjct: 68  RYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFNFPHAGSRFFGRELSSRAIESHKELVRGF 127

Query: 121 LRNAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGD 180
           L NAKEML EDGEIHITHKT YPFS+W I+KL    GL  +++  F    YPGY  KRG 
Sbjct: 128 LENAKEMLEEDGEIHITHKTTYPFSDWGIKKLGKGEGLKLLKKSKFELSHYPGYITKRGS 187

Query: 181 -GPDSDATFHIGSCSTFKFAK 194
            G  SD  F +G CST+ F +
Sbjct: 188 GGRRSDDYFPVGECSTYMFTQ 208

[Close]

BLAST of MC04g0422 vs. ExPASy Swiss-Prot
Match: O94480 (25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bmt5 PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.8e-22
Identity = 65/180 (36.11%), Postives = 99/180 (55.00%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFS-ACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHL 60
           ++ +  N + LL GEG+FSF+ + L     S   + ATS DSKE L +KY  A  ++  +
Sbjct: 51  VLPFEKNNRFLLLGEGNFSFAFSLLLHHVSSEGFVLATSYDSKEDLKQKYPDAAEYISKI 110

Query: 61  EVLGCTVVHEVDAGSMSQHLLLGLESFDRIF-NFPHAGFDLRERD----DVQIFLVGKFL 120
           E+ G  V+HE+DA  +  H  L  + FD IF NFPH+G  ++++D    D Q  L+  F 
Sbjct: 111 EINGGKVMHEIDATKLHLHKKLKTQKFDTIFWNFPHSGKGIKDQDRNILDNQKMLLA-FF 170

Query: 121 RNAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDG 175
           + +K +L+E G I IT     P++ WN++ LA   G   +  E F    YP Y ++R  G
Sbjct: 171 KASKFLLSEKGVIVITLAETKPYTLWNLKGLAKDAGYTSLMTEKFDSSFYPEYSHRRTIG 229

[Close]

BLAST of MC04g0422 vs. ExPASy Swiss-Prot
Match: Q3UY23 (Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus musculus OX=10090 GN=Fdxacb1 PE=2 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 9.1e-11
Identity = 58/198 (29.29%), Postives = 85/198 (42.93%), Query Frame = 0


Query: 8   QKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLEVLGCTVV 67
           +++LL GEG+FSF+A L      +  + AT    + +L       E +LK L   G  V 
Sbjct: 4   RRLLLVGEGNFSFAASLIDGLDPSVSVTATGFQHRAALEGDPVALE-NLKRLRERGVEVR 63

Query: 68  HEVDAGSMSQHLLLGLESFDRI-FNFPHAGFDLRERDDVQIFLVGKFLRNAKEMLAEDGE 127
             VD   +S  L      FDRI FNFPH G       + +  L+ KF ++  ++LA+ GE
Sbjct: 64  FGVDCTQLSHALPADDRDFDRIYFNFPHCGRKAGVAKNRE--LLAKFFQSCADVLAKAGE 123

Query: 128 IHIT-----------HKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 187
           +H+T                  + W +  +AA  G I  +   F     PGY  K     
Sbjct: 124 VHVTLCRGQGGTPADKPQREWHNSWQVVAMAALGGFILSDVCPFSCEAVPGY--KCTGYR 183

Query: 188 DSDATFHIGSCSTFKFAK 194
             D  FHI    T+ F +
Sbjct: 184 SQDRPFHIEGALTYIFTQ 196

[Close]

BLAST of MC04g0422 vs. ExPASy Swiss-Prot
Match: Q9BRP7 (Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX=9606 GN=FDXACB1 PE=1 SV=3)

HSP 1 Score: 63.9 bits (154), Expect = 2.2e-09
Identity = 54/188 (28.72%), Postives = 85/188 (45.21%), Query Frame = 0


Query: 8   QKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLEVLGCTVV 67
           +++LL GEG+FSF+A L+     +T++ AT L     L R    A  +L+ L   G  V 
Sbjct: 4   RRLLLVGEGNFSFAAALSETLDQSTQLTATCLQRPAELARD-PLAWENLQCLRERGIDVR 63

Query: 68  HEVDAGSMSQHLLLGLESFDRI-FNFPHAGFDLRERDDVQIFLVGKFLRNAKEMLAEDGE 127
             VD   ++    L    FD+I F FPH G       + +  L+ KF ++  ++LAE+GE
Sbjct: 64  FGVDCTQLADVFELHEREFDQIYFIFPHCGRKAGVAKNRE--LLAKFFQSCADVLAEEGE 123

Query: 128 IHIT-----------HKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 184
           +H+                   + W +  +AA  GLI  +   F  +   GY  K     
Sbjct: 124 VHVALCRGQGGTPADKPQREWHNSWQVVAMAALGGLILSDVYPFSCKAVAGY--KCTGYR 183

[Close]

BLAST of MC04g0422 vs. NCBI nr
Match: XP_022135928.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 348 bits (892), Expect = 1.19e-119
Identity = 177/205 (86.34%), Postives = 179/205 (87.32%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE
Sbjct: 8   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 67

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRIFNFPHAGFDLRERD------------DVQIF 120
           VLGCTVVHEVDAGSMSQHLLLGLESFDRI +  H      E +            D    
Sbjct: 68  VLGCTVVHEVDAGSMSQHLLLGLESFDRIASTSHMLVLTSESETMFKSCGFVLGCDTHKV 127

Query: 121 LVGKFLRNAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYE 180
           LVGKFLRNAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYE
Sbjct: 128 LVGKFLRNAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYE 187

Query: 181 NKRGDGPDSDATFHIGSCSTFKFAK 193
           NKRGDGPDSDATFHIGSCSTFKFAK
Sbjct: 188 NKRGDGPDSDATFHIGSCSTFKFAK 212

[Close]

BLAST of MC04g0422 vs. NCBI nr
Match: XP_028081873.1 (uncharacterized protein At4g26485 [Camellia sinensis])

HSP 1 Score: 240 bits (612), Expect = 3.99e-77
Identity = 118/198 (59.60%), Postives = 146/198 (73.74%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           I HYSS+QKILL GEGDFSF+ACLATAFGSA  + ATSLDS+ESL+ KY  A  +LK L+
Sbjct: 19  IKHYSSSQKILLVGEGDFSFAACLATAFGSALNMVATSLDSQESLMMKYPTASDNLKQLQ 78

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRI-FNFPHAGFDLRERDDVQIFL----VGKFLR 120
            LGCT++HE+D  +MS H  LG + FDRI FNFPHAGF L E   +QI L    V  FL 
Sbjct: 79  DLGCTILHEIDGTTMSLHPRLGFQLFDRIVFNFPHAGFTLSEHSSIQIMLHQDVVKGFLS 138

Query: 121 NAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
           +A  ML ++GE+H+THKT +PF+ W I+KLA  VGL  +E  +F + DYPGY NKRGDG 
Sbjct: 139 SASGMLTDNGEVHVTHKTAHPFNLWEIDKLAEEVGLCLVEEAWFSRYDYPGYVNKRGDGH 198

Query: 181 DSDATFHIGSCSTFKFAK 193
            S+ TF +G+C+TFKFAK
Sbjct: 199 RSNDTFPVGACTTFKFAK 216

[Close]

BLAST of MC04g0422 vs. NCBI nr
Match: XP_022139691.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 241 bits (614), Expect = 4.50e-77
Identity = 125/197 (63.45%), Postives = 140/197 (71.07%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           I HYSS+  ILL GEGDFSFS CLAT FGSA  + ATSLDSKESLL KY     +LK LE
Sbjct: 13  IKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVATNLKELE 72

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRI-FNFPHAGFDLRERDDVQI----FLVGKFLR 120
            LGCTV H+VDA SMSQH LL  +SFDR+ FNFPHAGF  +E D  QI     LV  FLR
Sbjct: 73  ELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRHLVRGFLR 132

Query: 121 NAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
           NAKE L E+GEIHITHKT +PFS W I +LA   GL+  E   F   +YP YENKRGDG 
Sbjct: 133 NAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYENKRGDGW 192

Query: 181 DSDATFHIGSCSTFKFA 192
           +SD TF +G+C TFKFA
Sbjct: 193 NSDGTFPVGACRTFKFA 209

[Close]

BLAST of MC04g0422 vs. NCBI nr
Match: KAF7141876.1 (hypothetical protein RHSIM_Rhsim06G0233700 [Rhododendron simsii])

HSP 1 Score: 237 bits (605), Expect = 4.62e-76
Identity = 124/198 (62.63%), Postives = 144/198 (72.73%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           I HYSSNQKILL GEGDFSF+ACLATAFGSA  + ATSLDSKE L+ K+  A  +L+ LE
Sbjct: 19  IKHYSSNQKILLVGEGDFSFAACLATAFGSAENMVATSLDSKELLMIKHQTASDNLEKLE 78

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRI-FNFPHAGFDLRERDDVQIFL----VGKFLR 120
            LGCT++HEVDA +MS    L  + FDRI FNFPHAGF LRE D  QI L    V  FLR
Sbjct: 79  GLGCTILHEVDAKTMSHDPRLVSKLFDRIVFNFPHAGFTLREHDAYQIELHQNVVKGFLR 138

Query: 121 NAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
           +A  ML +DGEIHITHKT YPF++W I+KLA  VGL  ++   F +  YPGY NKRGDG 
Sbjct: 139 SAHHMLEKDGEIHITHKTAYPFNKWEIKKLAEEVGLCLVDEIGFSRYHYPGYVNKRGDGS 198

Query: 181 DSDATFHIGSCSTFKFAK 193
            SD TF +G CSTF+FAK
Sbjct: 199 RSDCTFPVGECSTFRFAK 216

[Close]

BLAST of MC04g0422 vs. NCBI nr
Match: ONI11898.1 (hypothetical protein PRUPE_4G133300 [Prunus persica])

HSP 1 Score: 231 bits (588), Expect = 7.77e-73
Identity = 122/198 (61.62%), Postives = 141/198 (71.21%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           I HYSS QKILLAGEGDFSFS CLA AFG A  + ATSLDS+ESL+  Y++A  ++K LE
Sbjct: 10  IKHYSSYQKILLAGEGDFSFSVCLARAFGLAVNMVATSLDSRESLMLNYSKAMSNVKELE 69

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRI-FNFPHAGFDLRERDDVQIF----LVGKFLR 120
             GC V+HEVD  SMSQH  L    FDRI +NFPHAGF   ER+ +QI+    LV  FL+
Sbjct: 70  ARGCKVLHEVDVHSMSQHPFLIRVCFDRIIYNFPHAGFFSSERNRLQIWFHRDLVRGFLK 129

Query: 121 NAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
           NA EML   GEIH+THKT +PFSEW I +LA  VGL  ++ E F   DYPGYENKRG G 
Sbjct: 130 NACEMLTAIGEIHVTHKTTFPFSEWKIVELAKEVGLYLVDEEQFSLLDYPGYENKRGAGM 189

Query: 181 DSDATFHIGSCSTFKFAK 193
             D TFH+G CSTFKFAK
Sbjct: 190 -CDKTFHVGMCSTFKFAK 206

[Close]

BLAST of MC04g0422 vs. ExPASy TrEMBL
Match: A0A6J1C681 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111007760 PE=4 SV=1)

HSP 1 Score: 348 bits (892), Expect = 5.74e-120
Identity = 177/205 (86.34%), Postives = 179/205 (87.32%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE
Sbjct: 8   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 67

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRIFNFPHAGFDLRERD------------DVQIF 120
           VLGCTVVHEVDAGSMSQHLLLGLESFDRI +  H      E +            D    
Sbjct: 68  VLGCTVVHEVDAGSMSQHLLLGLESFDRIASTSHMLVLTSESETMFKSCGFVLGCDTHKV 127

Query: 121 LVGKFLRNAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYE 180
           LVGKFLRNAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYE
Sbjct: 128 LVGKFLRNAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYE 187

Query: 181 NKRGDGPDSDATFHIGSCSTFKFAK 193
           NKRGDGPDSDATFHIGSCSTFKFAK
Sbjct: 188 NKRGDGPDSDATFHIGSCSTFKFAK 212

[Close]

BLAST of MC04g0422 vs. ExPASy TrEMBL
Match: A0A6J1CDH1 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111010537 PE=4 SV=1)

HSP 1 Score: 241 bits (614), Expect = 2.18e-77
Identity = 125/197 (63.45%), Postives = 140/197 (71.07%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           I HYSS+  ILL GEGDFSFS CLAT FGSA  + ATSLDSKESLL KY     +LK LE
Sbjct: 13  IKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVATNLKELE 72

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRI-FNFPHAGFDLRERDDVQI----FLVGKFLR 120
            LGCTV H+VDA SMSQH LL  +SFDR+ FNFPHAGF  +E D  QI     LV  FLR
Sbjct: 73  ELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRHLVRGFLR 132

Query: 121 NAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
           NAKE L E+GEIHITHKT +PFS W I +LA   GL+  E   F   +YP YENKRGDG 
Sbjct: 133 NAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYENKRGDGW 192

Query: 181 DSDATFHIGSCSTFKFA 192
           +SD TF +G+C TFKFA
Sbjct: 193 NSDGTFPVGACRTFKFA 209

[Close]

BLAST of MC04g0422 vs. ExPASy TrEMBL
Match: A0A251PK16 (DUF2431 domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_4G133300 PE=4 SV=1)

HSP 1 Score: 231 bits (588), Expect = 3.76e-73
Identity = 122/198 (61.62%), Postives = 141/198 (71.21%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           I HYSS QKILLAGEGDFSFS CLA AFG A  + ATSLDS+ESL+  Y++A  ++K LE
Sbjct: 10  IKHYSSYQKILLAGEGDFSFSVCLARAFGLAVNMVATSLDSRESLMLNYSKAMSNVKELE 69

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRI-FNFPHAGFDLRERDDVQIF----LVGKFLR 120
             GC V+HEVD  SMSQH  L    FDRI +NFPHAGF   ER+ +QI+    LV  FL+
Sbjct: 70  ARGCKVLHEVDVHSMSQHPFLIRVCFDRIIYNFPHAGFFSSERNRLQIWFHRDLVRGFLK 129

Query: 121 NAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
           NA EML   GEIH+THKT +PFSEW I +LA  VGL  ++ E F   DYPGYENKRG G 
Sbjct: 130 NACEMLTAIGEIHVTHKTTFPFSEWKIVELAKEVGLYLVDEEQFSLLDYPGYENKRGAGM 189

Query: 181 DSDATFHIGSCSTFKFAK 193
             D TFH+G CSTFKFAK
Sbjct: 190 -CDKTFHVGMCSTFKFAK 206

[Close]

BLAST of MC04g0422 vs. ExPASy TrEMBL
Match: A0A0A0K734 (DUF2431 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G193720 PE=4 SV=1)

HSP 1 Score: 229 bits (583), Expect = 4.04e-73
Identity = 121/196 (61.73%), Postives = 140/196 (71.43%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           IMHYSS+  ILL GEGDFSFSACLATAF SA+ I ATSLDS++ L+ KYA+A R++K LE
Sbjct: 10  IMHYSSSHNILLVGEGDFSFSACLATAFASASNIIATSLDSRDELVMKYARAARNVKILE 69

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRI-FNFPHAGFDLRERDDVQIFL----VGKFLR 120
            LG TV+HEVDA +MSQH LL    FDRI FNFPHAGF  +E +  QI L    V  FLR
Sbjct: 70  ELGGTVLHEVDATTMSQHPLLHDMLFDRIVFNFPHAGFVYKESNTAQIELHRNLVRVFLR 129

Query: 121 NAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
           NAK M+  +GEIHITHKT +PFS+W I  LA   GL   E E F    YP YENKRG G 
Sbjct: 130 NAKSMIDVEGEIHITHKTSHPFSKWGIVNLACDEGLCLKESEIFHAWQYPNYENKRGHGQ 189

Query: 181 DSDATFHIGSCSTFKF 191
           + D TF +G+CSTFKF
Sbjct: 190 NPDGTFPVGACSTFKF 205

[Close]

BLAST of MC04g0422 vs. ExPASy TrEMBL
Match: M5X5P3 (Uncharacterized protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa026654mg PE=4 SV=1)

HSP 1 Score: 231 bits (588), Expect = 5.49e-73
Identity = 122/198 (61.62%), Postives = 141/198 (71.21%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           I HYSS QKILLAGEGDFSFS CLA AFG A  + ATSLDS+ESL+  Y++A  ++K LE
Sbjct: 10  IKHYSSYQKILLAGEGDFSFSVCLARAFGLAVNMVATSLDSRESLMLNYSKAMSNVKELE 69

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRI-FNFPHAGFDLRERDDVQIF----LVGKFLR 120
             GC V+HEVD  SMSQH  L    FDRI +NFPHAGF   ER+ +QI+    LV  FL+
Sbjct: 70  ARGCKVLHEVDVHSMSQHPFLIRVCFDRIIYNFPHAGFFSSERNRLQIWFHRDLVRGFLK 129

Query: 121 NAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
           NA EML   GEIH+THKT +PFSEW I +LA  VGL  ++ E F   DYPGYENKRG G 
Sbjct: 130 NACEMLTAIGEIHVTHKTTFPFSEWKIVELAKEVGLYLVDEEQFSLLDYPGYENKRGAGM 189

Query: 181 DSDATFHIGSCSTFKFAK 193
             D TFH+G CSTFKFAK
Sbjct: 190 -CDKTFHVGMCSTFKFAK 206

[Close]

BLAST of MC04g0422 vs. TAIR 10
Match: AT1G55790.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 196.4 bits (498), Expect = 2.1e-50
Identity = 104/196 (53.06%), Postives = 131/196 (66.84%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           + HYSSN +ILL GEGDFSFS  LAT FGSA+ I A+SLDS + ++RKY +A  +LK L+
Sbjct: 21  VKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKYKKARSNLKTLK 80

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDR-IFNFPHAGFDLRERDDVQI----FLVGKFLR 120
            LG  ++H VDA ++  H  L    FDR IFNFPHAGF  RE D   I     LV  F  
Sbjct: 81  RLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKHRELVFGFFN 140

Query: 121 NAKEMLAEDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
            A  +L  +GE+H++HK   PFSEWN+E+LA+R  L+ ++R  F K +YPGYENKRGDG 
Sbjct: 141 GASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYPGYENKRGDGR 200

Query: 181 DSDATFHIGSCSTFKF 192
             D  F +G CSTFKF
Sbjct: 201 RCDQPFLLGECSTFKF 216

[Close]

BLAST of MC04g0422 vs. TAIR 10
Match: AT5G56060.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 164.9 bits (416), Expect = 6.6e-41
Identity = 93/188 (49.47%), Postives = 117/188 (62.23%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           +  YS+ QKILL GEGDFSFS  LA  FGSAT I ATSLD++E L  KY   + +++ LE
Sbjct: 9   LQQYSNKQKILLVGEGDFSFSLSLARVFGSATNITATSLDTREELGIKYTDGKANVEGLE 68

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDR-IFNFPHAGFDLRERDDVQIF-----LVGKFL 120
           + GCTVVH V+  SMS    LG   +DR IFNFPH+G       D+        LV  FL
Sbjct: 69  LFGCTVVHGVNVHSMSSDYRLG--RYDRIIFNFPHSGLGFGSEHDIFFIMLHQGLVRGFL 128

Query: 121 RNAKEMLA-EDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGD 180
            +A++ML  EDGEIH+THKT  PF+ W IE LA   GL  +    F K  +PGY NK+G 
Sbjct: 129 ESARKMLKDEDGEIHVTHKTTDPFNRWGIETLAGEKGLRLIGEIEFHKWAFPGYSNKKGG 188

Query: 181 GPDSDATF 182
           G + ++TF
Sbjct: 189 GSNCNSTF 194

[Close]

BLAST of MC04g0422 vs. TAIR 10
Match: AT5G56075.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 150.2 bits (378), Expect = 1.7e-36
Identity = 86/198 (43.43%), Postives = 122/198 (61.62%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           + HY++ QKILL GEGDFSFS  LA AFGSA+ + ATSLD++  L +K+   + +++ LE
Sbjct: 67  LRHYTNKQKILLVGEGDFSFSLSLARAFGSASNLTATSLDTQGELEQKFKNGKANVEELE 126

Query: 61  VLGCTVVHEVDAGSM-SQHLLLGLESFDR-IFNFPHAGFDLRERDDVQIFLVGKFLRNAK 120
            LGC+VV+ V+  SM ++  + G   +DR IFNFP               LV  F+++A+
Sbjct: 127 RLGCSVVYGVNVHSMITKPSVGGSAIYDRVIFNFPTHE------------LVRGFMKSAR 186

Query: 121 EMLAED---GEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGP 180
            ++ ++   GEIH+ HKT YPFSEW ++ L  + GL  +    F    YPGY NKRG G 
Sbjct: 187 VLVKDEDKGGEIHVIHKTEYPFSEWKLKTLGEKEGLDLIREVEFCLSHYPGYFNKRGSGG 246

Query: 181 DSDATFHIGSCSTFKFAK 194
            SD++F +G  STF F K
Sbjct: 247 YSDSSFPVGKSSTFMFTK 252

[Close]

BLAST of MC04g0422 vs. TAIR 10
Match: AT5G25030.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 144.8 bits (364), Expect = 7.1e-35
Identity = 88/194 (45.36%), Postives = 115/194 (59.28%), Query Frame = 0


Query: 1   IMHYSSNQKILLAGEGDFSFSACLATAFGSATKIFATSLDSKESLLRKYAQAERHLKHLE 60
           +  YS+ QKIL+ GEG+FSFS  LA A GSAT I A SLD +E L R Y   + +++ LE
Sbjct: 9   LSRYSNEQKILVVGEGEFSFSLSLAKALGSATNITAISLDIREDLGRNYNNGKGNVEELE 68

Query: 61  VLGCTVVHEVDAGSMSQHLLLGLESFDRIFNFPHAGFDLRERDDVQIFLVGKFLRNAKEM 120
            LGCTVV  V+  SM     L       IFNFPHAG    +R+ V     G F+ +A+EM
Sbjct: 69  RLGCTVVRGVNVHSMKSDDRLAHYDI-IIFNFPHAG----KRNKV----FGGFMESAREM 128

Query: 121 LA-EDGEIHITHKTLYPFSEWNIEKLAARVGLIRMEREYFRKRDYPGYENKRGDGPDSDA 180
           +  EDGEIHIT  TL PF++W+++ LA   GL  ++R  F K  +P   NKR  G + D 
Sbjct: 129 MKDEDGEIHITLNTLNPFNKWDLKALAEESGLRLIQRMQFIKWAFPSSSNKRESGSNCDF 188

Query: 181 TFHIGSCSTFKFAK 194
            + IGS  T+ F K
Sbjct: 189 IYPIGSAITYMFKK 193

[Close]

BLAST of MC04g0422 vs. TAIR 10
Match: AT4G26485.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 141.4 bits (355), Expect = 7.8e-34
Identity = 84/168 (50.00%), Postives = 101/168 (60.12%), Query Frame = 0


Query: 34  IFATSLDSKESLLRKYAQAERHLKHLEVLGCTVVHEVDAGSMSQHLLLGLESFDRI-FNF 93
           I ATSLDS++ L  KY  A  ++  L+  GC + HEVD  +MS    L L+ +DRI FNF
Sbjct: 3   ITATSLDSEDELSIKYMDAVDNINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFNF 62

Query: 94  PHAG--FDLRERDDVQI----FLVGKFLRNAKEMLAEDGEIHITHKTLYPFSEWNIEKLA 153
           PHAG  F  RE     I     LV  FL NAKEML EDGEIHITHKT YPFS+W I+KL 
Sbjct: 63  PHAGSRFFGRELSSRAIESHKELVRGFLENAKEMLEEDGEIHITHKTTYPFSDWGIKKLG 122

Query: 154 ARVGLIRMEREYFRKRDYPGYENKRGD-GPDSDATFHIGSCSTFKFAK 194
              GL  +++  F    YPGY  KRG  G  SD  F +G CST+ F +
Sbjct: 123 KGEGLKLLKKSKFELSHYPGYITKRGSGGRRSDDYFPVGECSTYMFTQ 170

The following BLAST results are available for this feature:

BLAST of MC04g0422 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I1X0	2.9e-49	53.06	Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX...	[more]
P0C8L4	1.8e-46	53.73	Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 P...	[more]
O94480	1.8e-22	36.11	25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 9...	[more]
Q3UY23	9.1e-11	29.29	Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus mus...	[more]
Q9BRP7	2.2e-09	28.72	Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX...	[more]

BLAST of MC04g0422 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135928.1	1.19e-119	86.34	uncharacterized protein At4g26485-like [Momordica charantia]	[more]
XP_028081873.1	3.99e-77	59.60	uncharacterized protein At4g26485 [Camellia sinensis]	[more]
XP_022139691.1	4.50e-77	63.45	uncharacterized protein At4g26485-like [Momordica charantia]	[more]
KAF7141876.1	4.62e-76	62.63	hypothetical protein RHSIM_Rhsim06G0233700 [Rhododendron simsii]	[more]
ONI11898.1	7.77e-73	61.62	hypothetical protein PRUPE_4G133300 [Prunus persica]	[more]

BLAST of MC04g0422 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C681	5.74e-120	86.34	uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1CDH1	2.18e-77	63.45	uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A251PK16	3.76e-73	61.62	DUF2431 domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_4G133300 PE...	[more]
A0A0A0K734	4.04e-73	61.73	DUF2431 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G193720 PE=...	[more]
M5X5P3	5.49e-73	61.62	Uncharacterized protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa026654m...	[more]

BLAST of MC04g0422 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55790.1	2.1e-50	53.06	Domain of unknown function (DUF2431)	[more]
AT5G56060.1	6.6e-41	49.47	Domain of unknown function (DUF2431)	[more]
AT5G56075.1	1.7e-36	43.43	Domain of unknown function (DUF2431)	[more]
AT5G25030.1	7.1e-35	45.36	Domain of unknown function (DUF2431)	[more]
AT4G26485.1	7.8e-34	50.00	Domain of unknown function (DUF2431)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 42..62
None	No IPR available	PANTHER	PTHR11538:SF66	DUF2431 DOMAIN PROTEIN	coord: 1..193
None	No IPR available	PANTHER	PTHR11538	PHENYLALANYL-TRNA SYNTHETASE	coord: 1..193
IPR019446	Domain of unknown function DUF2431	PFAM	PF10354	DUF2431	coord: 11..171 e-value: 1.2E-41 score: 142.8
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 47..162

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0422.1	MC04g0422.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070475	rRNA base methylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0070042	rRNA (uridine-N3-)-methyltransferase activity