MC04g0390 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0390 vs. ExPASy Swiss-Prot
Match: Q07404 (Urease accessory protein UreH OS=Bacillus sp. (strain TB-90) OX=36824 GN=ureH PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.0e-10
Identity = 57/222 (25.68%), Postives = 94/222 (42.34%), Query Frame = 0

Query: 146 QSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIGCNRM-ESAAVGALWGCGHDAGQVIF 205
           Q +LL+    GF  G  H +  PDH+ A++ +     ++  S+  G  WG GH +  +IF
Sbjct: 3   QVDLLSILTLGFVLGIKHAME-PDHVIAVSTIVCQSKKLWRSSLAGVFWGIGHTSTLLIF 62

Query: 206 GLLFLLLKDKLHIEILRTWG---TVVVGVTLFIIGVMGIREASEVRTPCVALENGECDVG 265
           G+  +L+K K+  E    W      +VG+ L   G+  I    +      +  +   D  
Sbjct: 63  GMTIILMKKKISQE----WSMSLEFLVGIILVYFGISAILSLKKTHEHSHSRLHLHTDHP 122

Query: 266 IFETLDKPMVGKKKKLGFATFATGVVHGLQPDALMIVLPALALPSRIAGAAFLVMFLVGT 325
           I+     P V         +   G++HGL   A M++L    +     G  +++ F  GT
Sbjct: 123 IYTYKGIPYV--------KSLFIGIIHGLAGSAAMVLLTMSTVEKAWEGLLYILFFGAGT 182

Query: 326 VISMGSYTAFIGSFSEALKDRVPRITERLTWAASFVAIALGL 364
           V+ M S+T  IG     L  R  RI         F++   G+
Sbjct: 183 VLGMLSFTTLIG-IPFTLSARKIRIHNAFIQITGFISTVFGI 210

BLAST of MC04g0390 vs. NCBI nr
Match: XP_022135872.1 (uncharacterized protein LOC111007714 [Momordica charantia])

HSP 1 Score: 718 bits (1854), Expect = 1.28e-260
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60
           MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS
Sbjct: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60

Query: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120
           SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP
Sbjct: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120

Query: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240
           CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI
Sbjct: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240

Query: 241 REASEVRTPCVALENGECDVGIFETLDKPMVGKKKKLGFATFATGVVHGLQPDALMIVLP 300
           REASEVRTPCVALENGECDVGIFETLDKPMVGKKKKLGFATFATGVVHGLQPDALMIVLP
Sbjct: 241 REASEVRTPCVALENGECDVGIFETLDKPMVGKKKKLGFATFATGVVHGLQPDALMIVLP 300

Query: 301 ALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASFVAIA 360
           ALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASFVAIA
Sbjct: 301 ALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASFVAIA 360

Query: 361 LGLAIIISQFLGYSLY 376
           LGLAIIISQFLGYSLY
Sbjct: 361 LGLAIIISQFLGYSLY 376

BLAST of MC04g0390 vs. NCBI nr
Match: XP_023529731.1 (uncharacterized protein LOC111792465 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 623 bits (1606), Expect = 9.76e-223
Identity = 331/382 (86.65%), Postives = 349/382 (91.36%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60
           M+RLLYSPSPTS KINP+  L LPRFR I SAKLNFP+PTRHGLR +GL S K EKP + 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKTEKPSMP 60

Query: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120
            I  LS  NSSSDSI  SDSRA SPK DLLEHF GK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRAGSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AA ASF NAAKTG PAAAAVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240
           C+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGT+VVGVTLF+IGV+GI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240

Query: 241 REASEVRTPCVA-LENGECDVGIFETLDKPMV-----GKKKKLGFATFATGVVHGLQPDA 300
           REASEVRTPCV  LENGECDVGI++TL+KPMV      KKKK+GFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360
           LMIVLPALALPSR+AGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 376
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of MC04g0390 vs. NCBI nr
Match: XP_022989236.1 (uncharacterized protein LOC111486376 [Cucurbita maxima])

HSP 1 Score: 621 bits (1602), Expect = 3.97e-222
Identity = 330/382 (86.39%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60
           M+RLLYSPSPTS KINP+  L LPRFR I S KLNFP+PTRHGLR +GL+S K EKP + 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSVKLNFPLPTRHGLRPLGLLSSKVEKPSMP 60

Query: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120
            I  LS  NSSSDSI  SDSRA+SPK DLLEHF GK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRASSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AA ASF NAAKTG PAAAAVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240
           C+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGT+VVGVTLF+IGV+GI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240

Query: 241 REASEVRTPCVA-LENGECDVGIFETLDKPMV-----GKKKKLGFATFATGVVHGLQPDA 300
           REASEVRTPCV  LENGECDVGI++TL+KPMV      KKKK+GFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360
           LMIVLPALALPSR+AGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 376
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of MC04g0390 vs. NCBI nr
Match: KAG6589014.1 (hypothetical protein SDJN03_17579, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015277.1 ureH [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617 bits (1592), Expect = 1.32e-220
Identity = 328/382 (85.86%), Postives = 348/382 (91.10%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60
           M+RLLYSPSPTS KINP+  L LPRFR I SAKLNFP+PTRHGLR +GL S K +KP + 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60

Query: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120
            I  LS  NSSSDSI  SDSRA SPK DLLEHF GK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AA ASF NAAKTG PAAAAVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240
           C+RMESAAVGALWGCGHDAGQ+IFGLLFLLLKDKLHIEILRTWGT+VVGVTLF+IGV+GI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240

Query: 241 REASEVRTPCVA-LENGECDVGIFETLDKPMV-----GKKKKLGFATFATGVVHGLQPDA 300
           REASEVRTPCV  LENGECDVGI++TL+ PMV      KKKK+GFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360
           LMIVLPALALPSR+AGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 376
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of MC04g0390 vs. NCBI nr
Match: XP_022928346.1 (uncharacterized protein LOC111435202 [Cucurbita moschata])

HSP 1 Score: 614 bits (1583), Expect = 3.10e-219
Identity = 326/382 (85.34%), Postives = 347/382 (90.84%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60
           M+RLLYSPSPTS KINP+  L LPRFR I SAKLNFP+PTRHGLR +GL S K +KP + 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60

Query: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120
            I  LS  NS SDSI  S+SRA SPK DLLEHF GK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSLSDSISTSNSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AA ASF NAAKTG PAAAAVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240
           C+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGT+VVGVTLF+IG++GI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGILGI 240

Query: 241 REASEVRTPCVA-LENGECDVGIFETLDKPMV-----GKKKKLGFATFATGVVHGLQPDA 300
           REASEVRTPCV  LENGECDVGI++TL+ PMV      KKKK+GFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360
           LMIVLPALALPSR+AGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 376
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of MC04g0390 vs. ExPASy TrEMBL
Match: A0A6J1C3Z9 (uncharacterized protein LOC111007714 OS=Momordica charantia OX=3673 GN=LOC111007714 PE=4 SV=1)

HSP 1 Score: 718 bits (1854), Expect = 6.21e-261
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60
           MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS
Sbjct: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60

Query: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120
           SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP
Sbjct: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120

Query: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240
           CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI
Sbjct: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240

Query: 241 REASEVRTPCVALENGECDVGIFETLDKPMVGKKKKLGFATFATGVVHGLQPDALMIVLP 300
           REASEVRTPCVALENGECDVGIFETLDKPMVGKKKKLGFATFATGVVHGLQPDALMIVLP
Sbjct: 241 REASEVRTPCVALENGECDVGIFETLDKPMVGKKKKLGFATFATGVVHGLQPDALMIVLP 300

Query: 301 ALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASFVAIA 360
           ALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASFVAIA
Sbjct: 301 ALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASFVAIA 360

Query: 361 LGLAIIISQFLGYSLY 376
           LGLAIIISQFLGYSLY
Sbjct: 361 LGLAIIISQFLGYSLY 376

BLAST of MC04g0390 vs. ExPASy TrEMBL
Match: A0A6J1JJH7 (uncharacterized protein LOC111486376 OS=Cucurbita maxima OX=3661 GN=LOC111486376 PE=4 SV=1)

HSP 1 Score: 621 bits (1602), Expect = 1.92e-222
Identity = 330/382 (86.39%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60
           M+RLLYSPSPTS KINP+  L LPRFR I S KLNFP+PTRHGLR +GL+S K EKP + 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSVKLNFPLPTRHGLRPLGLLSSKVEKPSMP 60

Query: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120
            I  LS  NSSSDSI  SDSRA+SPK DLLEHF GK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRASSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AA ASF NAAKTG PAAAAVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240
           C+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGT+VVGVTLF+IGV+GI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240

Query: 241 REASEVRTPCVA-LENGECDVGIFETLDKPMV-----GKKKKLGFATFATGVVHGLQPDA 300
           REASEVRTPCV  LENGECDVGI++TL+KPMV      KKKK+GFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360
           LMIVLPALALPSR+AGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 376
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of MC04g0390 vs. ExPASy TrEMBL
Match: A0A6J1EK24 (uncharacterized protein LOC111435202 OS=Cucurbita moschata OX=3662 GN=LOC111435202 PE=4 SV=1)

HSP 1 Score: 614 bits (1583), Expect = 1.50e-219
Identity = 326/382 (85.34%), Postives = 347/382 (90.84%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60
           M+RLLYSPSPTS KINP+  L LPRFR I SAKLNFP+PTRHGLR +GL S K +KP + 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60

Query: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120
            I  LS  NS SDSI  S+SRA SPK DLLEHF GK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSLSDSISTSNSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AA ASF NAAKTG PAAAAVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240
           C+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGT+VVGVTLF+IG++GI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGILGI 240

Query: 241 REASEVRTPCVA-LENGECDVGIFETLDKPMV-----GKKKKLGFATFATGVVHGLQPDA 300
           REASEVRTPCV  LENGECDVGI++TL+ PMV      KKKK+GFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360
           LMIVLPALALPSR+AGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 376
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of MC04g0390 vs. ExPASy TrEMBL
Match: A0A6J1I158 (uncharacterized protein LOC111468477 OS=Cucurbita maxima OX=3661 GN=LOC111468477 PE=4 SV=1)

HSP 1 Score: 594 bits (1532), Expect = 8.13e-212
Identity = 321/380 (84.47%), Postives = 337/380 (88.68%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60
           MERLLYSPSPTSLKIN + SL L     I S KLNFP+PT HGL+ +GL  CKNEKP +S
Sbjct: 1   MERLLYSPSPTSLKINLNSSLTLLWSGRIDSLKLNFPLPTLHGLKPLGLTFCKNEKPSMS 60

Query: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120
           S   LS  N  SDSI PSDS A SPK D LEHF GKEKVKRSL VLSAL LILIQP  AP
Sbjct: 61  SFCCLSSSNWLSDSIFPSDSLAGSPKPDFLEHFAGKEKVKRSLCVLSALVLILIQPGLAP 120

Query: 121 AASASFPNAA-KTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSI 180
           AA ASF NAA KTG PAAA VGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 AAFASFQNAAAKTGGPAAAVVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 181 GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMG 240
           GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGT+VVG+TLFIIGVMG
Sbjct: 181 GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGMTLFIIGVMG 240

Query: 241 IREASEVRTPCVALENGECDVGIFETLDKPMV---GKKKKLGFATFATGVVHGLQPDALM 300
           IRE SEVRTPCVALENGECDVGI+ETL+KPMV    KKKK+GFATFATGVVHGLQPDALM
Sbjct: 241 IREVSEVRTPCVALENGECDVGIYETLEKPMVVGKKKKKKIGFATFATGVVHGLQPDALM 300

Query: 301 IVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASF 360
           IVLPALALPSR+AGAAFL+MFLVGTV+SMGSYTAFIGSFSEALKDRVPRITERLTWAASF
Sbjct: 301 IVLPALALPSRVAGAAFLLMFLVGTVLSMGSYTAFIGSFSEALKDRVPRITERLTWAASF 360

Query: 361 VAIALGLAIIISQFLGYSLY 376
           VAIALGLAIII+ FLG+SLY
Sbjct: 361 VAIALGLAIIITHFLGFSLY 380

BLAST of MC04g0390 vs. ExPASy TrEMBL
Match: A0A1S3BJI9 (uncharacterized protein LOC103490284 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490284 PE=4 SV=1)

HSP 1 Score: 585 bits (1507), Expect = 5.59e-208
Identity = 317/382 (82.98%), Postives = 338/382 (88.48%), Query Frame = 0

Query: 1   MERLL-YSPSPTSLKINPSRSLFLPRF-RLIGSAKLNFPIPTRHGLRSIGLISCKNEKPP 60
           MERLL YS SPTSL INP+ SL LPRF R+I S + NFP+PTRHG   +G +S KN+KP 
Sbjct: 1   MERLLLYSSSPTSLNINPNSSLVLPRFARIIHSLRPNFPLPTRHGFHPLGFLSFKNDKPS 60

Query: 61  LSSIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVF 120
            SSI   S  NSS+DSI  SDS   SPK D L+ F  KEKVK SLFVLSALALILIQPVF
Sbjct: 61  FSSIPCFSSPNSSADSIFQSDSPPDSPKPDFLKPFARKEKVKSSLFVLSALALILIQPVF 120

Query: 121 APAASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAA ASF NAAKTG PAA AVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVM 240
           IGC+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGT++VGVTLF+IG+M
Sbjct: 181 IGCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIM 240

Query: 241 GIREASEVRTP-CVALENGECDVGIFETLDKPMV---GKKKKLGFATFATGVVHGLQPDA 300
           GIREASE+RTP  VAL+NGECDVGI+ETL+KPMV    K KKLGFATFATGVVHGLQPDA
Sbjct: 241 GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360
           LMIVLPALALPSR+AGAAFL+MFLVGTVISMGSYTAFIG+FSEALKDRVPRITERLTW A
Sbjct: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGTFSEALKDRVPRITERLTWVA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 376
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of MC04g0390 vs. TAIR 10
Match: AT2G16800.1 (high-affinity nickel-transport family protein )

HSP 1 Score: 391.3 bits (1004), Expect = 8.5e-109
Identity = 229/387 (59.17%), Postives = 277/387 (71.58%), Query Frame = 0

Query: 1   MERLLYSPS-----PTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNE 60
           M+RLL  PS     P+  +  PS  L     RL  +    FP   R   R I  ISC   
Sbjct: 1   MDRLLQPPSSHSIAPSKFQSRPSPLLL---HRLHSTNLSTFPSSRRLESRRISSISCFFR 60

Query: 61  KPPLSSIRSLSLFNSSSDSICPSDSRAASPKLDLLEH----FGGKEKVKRSLFVL-SALA 120
           + P S   S  L  SS+  I  S + A+ P    ++     F  ++ +   + +L SA+A
Sbjct: 61  QNP-SPDTSPGLNQSSNFLIASSQTDASKPNPGFIQRIVSSFEQRKTISAGMVILVSAIA 120

Query: 121 LILIQPVFAPAASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDH 180
            +L+ P+  P A ASF  A  +G   AA VG +L+++E+L SAWTGFFAGCLHTLSGPDH
Sbjct: 121 ALLLNPILVPPAFASFQTATNSG--GAAVVGGKLLRTEVLTSAWTGFFAGCLHTLSGPDH 180

Query: 181 LAALAPLSIGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGV 240
           LAALAPLSIG  RMESAAVGALWGCGHDAGQ+IFGLLFLLLKD+LHIE++RTWGT VVG+
Sbjct: 181 LAALAPLSIGRTRMESAAVGALWGCGHDAGQLIFGLLFLLLKDRLHIEVIRTWGTRVVGL 240

Query: 241 TLFIIGVMGIREASEVRTPC-VALENGECDVGIFETLDKPMVGKKKKLGFATFATGVVHG 300
           TL +IG MGI+EASE+  PC V LENGE D       +K +  KKKK+GFATFATG+VHG
Sbjct: 241 TLLVIGAMGIKEASEMPEPCVVTLENGETD-------EKSL--KKKKIGFATFATGIVHG 300

Query: 301 LQPDALMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITER 360
           LQPDALM+VLPALALPSRIAGA+FL+MFL+GTVI+MGSYT FIGS SEALK++VPRITE+
Sbjct: 301 LQPDALMMVLPALALPSRIAGASFLIMFLIGTVIAMGSYTVFIGSCSEALKEKVPRITEK 360

Query: 361 LTWAASFVAIALGLAIIISQFLGYSLY 377
           LTWA+S VAI LGLAII+SQF G+SLY
Sbjct: 361 LTWASSLVAIGLGLAIIVSQFFGFSLY 372

BLAST of MC04g0390 vs. TAIR 10
Match: AT4G35080.1 (high-affinity nickel-transport family protein )

HSP 1 Score: 379.0 bits (972), Expect = 4.4e-105
Identity = 225/387 (58.14%), Postives = 274/387 (70.80%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSR-----SLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNE 60
           MERLL  PS +S  I+PS+     S FLPR R   S+ L+F    R   R +  ISC + 
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLR---SSGLSFVSTHRPESRRVSSISCNSS 60

Query: 61  K-PPLSSIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKR----SLFVLSALA 120
           + P L +    +  N+S +    SD    +P           E+ K     ++ ++SA+A
Sbjct: 61  QIPSLYTPIGSNTTNNSFNGSPKSDESKPNPGFLTRIATSASEQRKTLSTGTVILISAVA 120

Query: 121 LILIQPVFAPAASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDH 180
           ++L+ P+ AP A ASF  AAK+G                L SAWTGF AGCLHTLSGPDH
Sbjct: 121 VLLLNPLLAPPAFASFQTAAKSG---------------WLTSAWTGFLAGCLHTLSGPDH 180

Query: 181 LAALAPLSIGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGV 240
           LAALAPLSIG ++MESAAVGALWGCGHDAGQVIFGLLFLLLKD+LHIE+L+TWGT +VG+
Sbjct: 181 LAALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQTWGTRIVGL 240

Query: 241 TLFIIGVMGIREASEVRTPCVALENGECDVGIFETLDKPM-VGKKKKLGFATFATGVVHG 300
           TL IIG MGI+EASE+  PCVALE    D+ +  T  + + + KKKK+GFATFATGVVHG
Sbjct: 241 TLVIIGAMGIKEASEIPEPCVALET---DISMVSTEKEALPLPKKKKIGFATFATGVVHG 300

Query: 301 LQPDALMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITER 360
           LQPDALMIVLPALALPSR+AG+AFL+MFLVGTVI+MGSYTAFIGS SEALK++VPRITE+
Sbjct: 301 LQPDALMIVLPALALPSRLAGSAFLIMFLVGTVIAMGSYTAFIGSCSEALKEKVPRITEK 360

Query: 361 LTWAASFVAIALGLAIIISQFLGYSLY 377
           LTW +S VAI LGL I+IS F G+SLY
Sbjct: 361 LTWVSSLVAIGLGLGIVISPFFGFSLY 365

BLAST of MC04g0390 vs. TAIR 10
Match: AT4G35080.3 (high-affinity nickel-transport family protein )

HSP 1 Score: 374.8 bits (961), Expect = 8.2e-104
Identity = 229/411 (55.72%), Postives = 283/411 (68.86%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSR-----SLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNE 60
           MERLL  PS +S  I+PS+     S FLPR R   S+ L+F    R   R +  ISC + 
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLR---SSGLSFVSTHRPESRRVSSISCNSS 60

Query: 61  K-----PPLSSIRSLSLFNSS--SDSICPSD-------SRAASPKLDLLEHFGG---KEK 120
           +      P+ S  + + FN S  SD   P+        + A+  + +  E F     ++K
Sbjct: 61  QIPSLYTPIGSNTTNNSFNGSPKSDESKPNPGFLTRIATSASEQRKETYEKFVYSLLEKK 120

Query: 121 VKR------------SLFVLSALALILIQPVFAPAASASFPNAAKTGAPAAAAVGRRLIQ 180
           + R            ++ ++SA+A++L+ P+ AP A ASF  AAK+G             
Sbjct: 121 IPRITKSSNFTLSTGTVILISAVAVLLLNPLLAPPAFASFQTAAKSG------------- 180

Query: 181 SELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIGCNRMESAAVGALWGCGHDAGQVIFGL 240
              L SAWTGF AGCLHTLSGPDHLAALAPLSIG ++MESAAVGALWGCGHDAGQVIFGL
Sbjct: 181 --WLTSAWTGFLAGCLHTLSGPDHLAALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGL 240

Query: 241 LFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGIREASEVRTPCVALENGECDVGIFETL 300
           LFLLLKD+LHIE+L+TWGT +VG+TL IIG MGI+EASE+  PCVALE    D+ +  T 
Sbjct: 241 LFLLLKDRLHIEVLQTWGTRIVGLTLVIIGAMGIKEASEIPEPCVALET---DISMVSTE 300

Query: 301 DKPM-VGKKKKLGFATFATGVVHGLQPDALMIVLPALALPSRIAGAAFLVMFLVGTVISM 360
            + + + KKKK+GFATFATGVVHGLQPDALMIVLPALALPSR+AG+AFL+MFLVGTVI+M
Sbjct: 301 KEALPLPKKKKIGFATFATGVVHGLQPDALMIVLPALALPSRLAGSAFLIMFLVGTVIAM 360

Query: 361 GSYTAFIGSFSEALKDRVPRITERLTWAASFVAIALGLAIIISQFLGYSLY 377
           GSYTAFIGS SEALK++VPRITE+LTW +S VAI LGL I+IS F G+SLY
Sbjct: 361 GSYTAFIGSCSEALKEKVPRITEKLTWVSSLVAIGLGLGIVISPFFGFSLY 389

BLAST of MC04g0390 vs. TAIR 10
Match: AT4G35080.2 (high-affinity nickel-transport family protein )

HSP 1 Score: 320.1 bits (819), Expect = 2.4e-87
Identity = 201/387 (51.94%), Postives = 246/387 (63.57%), Query Frame = 0

Query: 1   MERLLYSPSPTSLKINPSR-----SLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNE 60
           MERLL  PS +S  I+PS+     S FLPR R   S+ L+F    R   R +  ISC + 
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLR---SSGLSFVSTHRPESRRVSSISCNSS 60

Query: 61  K-PPLSSIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKR----SLFVLSALA 120
           + P L +    +  N+S +    SD    +P           E+ K     ++ ++SA+A
Sbjct: 61  QIPSLYTPIGSNTTNNSFNGSPKSDESKPNPGFLTRIATSASEQRKTLSTGTVILISAVA 120

Query: 121 LILIQPVFAPAASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDH 180
           ++L+ P+ AP A ASF  AAK+G                L SAWTGF AGCLHTLSGPDH
Sbjct: 121 VLLLNPLLAPPAFASFQTAAKSG---------------WLTSAWTGFLAGCLHTLSGPDH 180

Query: 181 LAALAPLSIGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGV 240
           LAALAPLSIG ++MESAAVGALWGCGHDAGQVIFGLLFLLLKD+LHIE+L+TWGT +VG+
Sbjct: 181 LAALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQTWGTRIVGL 240

Query: 241 TLFIIGVMGIREASEVRTPCVALENGECDVGIFETLDKPM-VGKKKKLGFATFATGVVHG 300
           TL IIG MGI+EASE+  PCVALE    D+ +  T  + + + KKKK+GFATFATGVVHG
Sbjct: 241 TLVIIGAMGIKEASEIPEPCVALET---DISMVSTEKEALPLPKKKKIGFATFATGVVHG 300

Query: 301 LQPDALMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITER 360
           LQPDALMIVLPALALPSR                             EALK++VPRITE+
Sbjct: 301 LQPDALMIVLPALALPSR-----------------------------EALKEKVPRITEK 336

Query: 361 LTWAASFVAIALGLAIIISQFLGYSLY 377
           LTW +S VAI LGL I+IS F G+SLY
Sbjct: 361 LTWVSSLVAIGLGLGIVISPFFGFSLY 336