MC04g0389 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0389
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionGlycerate dehydrogenase
LocationMC04: 3113647 .. 3116753 (-)
RNA-Seq ExpressionMC04g0389
SyntenyMC04g0389
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCAGCTTATCCAAGAGCCCATTGTATAAGGCCCAACATTTTGGACCAAAACCATAGGCAAGGATTGGGTTTAAAAGGCCCAATAGAGCCGGGGGTATCTTCGTCATTTTGATCGATAAGGATATAGCAGGGACTAACGTGGCGCGAATTTAAAAGGCCATATCGTTATTATCCATTTTCCACCAAAACGAGAGGTCCAACAACTCCCATCCTGAGGCAAAGCAGAGACACAAATTCCCCCTTCCATAATCCTCTACAACAGAGCCCATCCAAATCACCCTCCATCCCAATCAGGTCAAACAGTCAAACAGTCAAACATACCAAAAGTCAACAGTCAGCATTTCCGCAAAAATTCCACCGCTCATCGGAATTTCCCTCACGTACTCCGCGTCGCACTTGATTATTATCCGGTACTCGCCACGAGCCACCCGAAATTTTAAAACCGAACTGAAAGCTTCAGCAGATGGCACGCTTCAGTGGAGAAGAGCATCGAAAAATTTGTCTCCCATGGCGAAGCCCGTTCAAATCGAAGTGTGGAACCCGAATGGGAAGTACAGGGTTGTGAGCACCAAGCCCATGCCTGGAACTCGCTGGATCAATCTCTTGATTGAGCAAGATTGCCGACTCGAAGTAATTTTTTTCTTTTCTCTCTTTGTTGTTTTCGCCTATTTCTGATTCTTTCGCCGGGAATTTTGTCCGACGGTGTTCATTGAAGTTTTTGCATCCGGTTTGCGGTTTTAGATTTGTACTGAGAAGAAGACGATTCTGTCTGTGGATGATATTCTCGCTCTCATCGGCGATAAGTGCGATGGCGTTATTGGACAGGTACCGACGGCTTCCTTGTTGGAACCAATTCTTGTTAATCAGAAAGTTTTCATGCATTTCAATTAATGAGAACCCTAGACAGTAAGAATTTTGAATTAGAAGTAACATTTCAATACAATAACTAGTTAGATGGCTTCTCCAGATGATGAGTAGAAGTTGGAAAAATTACAAATGTGTGTCTGTATTTGTGATTCTGAGATTTGTTTTCTTATATGTTACAGTTGACTGAAGATTGGGGAGAGGTGCTGTTTTCTGCACTGAGCAGAGCAGGAGGGAAAGCTTTTAGTAATATGGCTGTTGGTTACAATAATGTTGATGTTAATGCTGCTAATAAGTATGGTGTTGCTGTAGGAAACACTCCAGTAAGTTTATTTTTGATGTACAAAATGCTACAGGCTCCCCTGCTGATCCCTTGAATACCTGAATTTGATATTGGGCATTTCTTAATTTTAATGCTCTGTTACGTATTTACATATTTGATCCTTAGACAGGCATTTAGATCATTCATTTCTTACTTGTTTAGGGAGTGCTTACAGAAACTACAGCAGAGTTGGCAGCTTCACTTTCTCTTGCAGCTGCAAGAAGGATAGTTGAAGCTGATGAGTTCATGAGGGCAGGTCTCTATGAGGGATGGCTTCCCAATTTGTATGATTATCTACCATGAGTTGAATTTTTCTTTAGAAATAGGCAACCAAAATGGCCCAACAAATTTGAAATAATTTCTTTTGAATAGGTTTGTTGGAAACTTGCTGAAAGGGCAGACTGTGGGTGTGATTGGAGCGGGTCGTATTGGATCTGCTTATGCAAGAATGATGGTACTTTTACTAACTCAATTAATCATGAATTTGATTATTCAACTTTTCAACCGGAAATTCTTTTGTTTCTAAATCTGATTCCAAAAATGTTGTTGTCTTTCCCGAGGAAGAGGATCGTTAGGATTTCAAATGAAATGTTGTCATCAATAATTGGTCTTGGATTAAAAGGTATAATGACTCTTTTCCCTTGTTGATGATTTCTCGTTCTATTCTTGCAGGTAGAAGGATTCAAGATGAACTTAATCTACTATGATCTTTACCAGGCAACCAGACTCGAAAAGTTCGTTACAGGCATGTCTCGATCAACAATTCTTGAAAGCAACGAGTTTGAATTTCTTATGCTGAAATGCTTGTTGAATTGCAGCCTATGGTGAGTTCCTGGTAGCCAACGGTGAGGCACCCGTGTCATGGAAAAGAGCAACATCCATGGATGAGGTGCTCCGAGAGGCCGATGTGGTATGACTTTGATACAATCAATTAACTTGGTTTTGATGAGCTCTTGTTTCCATGTATTATGTATATAAATTAGTTATTGTGTTCTCCTTTGAGTTTCTAGATAAGTCTTCATCCAGTACTCGATAAAACCACTTACCATCTGGTGAACAAAGAAAGTCTTAAAGCCATGAAGAAGGTGAAGTTTGCTAAAGCTCTTCTCTCCCTTCAATTATTCTGTTGATCTCCCCAATCCCATGACAGGGATTCCTGATTGATTGCATTTAAACTTGTGTTTCTTTAAACTATGTTCTTTTTTGTTTTTTTTTTCTTTGGAGTACAATTCTTCAAACTATGTTAAAAATGCAGGATGCAATCCTTATCAACTGTAGTAGGGGACCTGTCATCGACGAAGTAGCCCTTGTCGAGCATTTAAAAGAGAATCCAATGTTTCGAGTAGGCCTTGATGTCTTTGAGGTATTTCTACTCATAAATCCCTCGGCTTCTCATCTTAAAAATAGACTCACAACTCACTATGATCATAATCAAGTGAATACGCGTCCTGATTCATCACTCTACTGCATTAGGATGAACCATACATGAAACCCGGGCTTGCTGATATGAAGAACGCGGTTATTGTTCCTCACATTGCTTCCGCTTCCAAGGTAAATCTCTGCCTAGCCTTACAGTGAAATGAAGATCGACTTACAGAAGCATTAACCCCGCTTTAATAACTATCATGTAGTGGACTCGTGAAGGAATGGCGACATTGGCTGCTCTTAATGTCCTGGTGCGTGCAACCTGGAAATTTATACTCGCCTCAGAAAAACATTAGATTTCATTTGGATTCTTCTTCTGATCTTGCTTGCATTTTGAGTTCAAAACACAACTAAAACTCTGGCTCTCTAACCACAGGGAACGATCAAAGATTATCCTGTTTGGGCCGATCCAAACCGGGTAGAACAGTTCCTTGACGAGAACATTTCCCCTCCAGCTGCATCTCCGAGCATTGTGAATGCAAAAGCCTTGGGTAAT

mRNA sequence

GTCAGCTTATCCAAGAGCCCATTGTATAAGGCCCAACATTTTGGACCAAAACCATAGGCAAGGATTGGGTTTAAAAGGCCCAATAGAGCCGGGGGTATCTTCGTCATTTTGATCGATAAGGATATAGCAGGGACTAACGTGGCGCGAATTTAAAAGGCCATATCGTTATTATCCATTTTCCACCAAAACGAGAGGTCCAACAACTCCCATCCTGAGGCAAAGCAGAGACACAAATTCCCCCTTCCATAATCCTCTACAACAGAGCCCATCCAAATCACCCTCCATCCCAATCAGGTCAAACAGTCAAACAGTCAAACATACCAAAAGTCAACAGTCAGCATTTCCGCAAAAATTCCACCGCTCATCGGAATTTCCCTCACGTACTCCGCGTCGCACTTGATTATTATCCGGTACTCGCCACGAGCCACCCGAAATTTTAAAACCGAACTGAAAGCTTCAGCAGATGGCACGCTTCAGTGGAGAAGAGCATCGAAAAATTTGTCTCCCATGGCGAAGCCCGTTCAAATCGAAGTGTGGAACCCGAATGGGAAGTACAGGGTTGTGAGCACCAAGCCCATGCCTGGAACTCGCTGGATCAATCTCTTGATTGAGCAAGATTGCCGACTCGAAATTTGTACTGAGAAGAAGACGATTCTGTCTGTGGATGATATTCTCGCTCTCATCGGCGATAAGTGCGATGGCGTTATTGGACAGTTGACTGAAGATTGGGGAGAGGTGCTGTTTTCTGCACTGAGCAGAGCAGGAGGGAAAGCTTTTAGTAATATGGCTGTTGGTTACAATAATGTTGATGTTAATGCTGCTAATAAGTATGGTGTTGCTGTAGGAAACACTCCAGGAGTGCTTACAGAAACTACAGCAGAGTTGGCAGCTTCACTTTCTCTTGCAGCTGCAAGAAGGATAGTTGAAGCTGATGAGTTCATGAGGGCAGGTCTCTATGAGGGATGGCTTCCCAATTTGTTTGTTGGAAACTTGCTGAAAGGGCAGACTGTGGGTGTGATTGGAGCGGGTCGTATTGGATCTGCTTATGCAAGAATGATGGTAGAAGGATTCAAGATGAACTTAATCTACTATGATCTTTACCAGGCAACCAGACTCGAAAAGTTCGTTACAGCCTATGGTGAGTTCCTGGTAGCCAACGGTGAGGCACCCGTGTCATGGAAAAGAGCAACATCCATGGATGAGGTGCTCCGAGAGGCCGATGTGATAAGTCTTCATCCAGTACTCGATAAAACCACTTACCATCTGGTGAACAAAGAAAGTCTTAAAGCCATGAAGAAGGATGCAATCCTTATCAACTGTAGTAGGGGACCTGTCATCGACGAAGTAGCCCTTGTCGAGCATTTAAAAGAGAATCCAATGTTTCGAGTAGGCCTTGATGTCTTTGAGGATGAACCATACATGAAACCCGGGCTTGCTGATATGAAGAACGCGGTTATTGTTCCTCACATTGCTTCCGCTTCCAAGTGGACTCGTGAAGGAATGGCGACATTGGCTGCTCTTAATGTCCTGGGAACGATCAAAGATTATCCTGTTTGGGCCGATCCAAACCGGGTAGAACAGTTCCTTGACGAGAACATTTCCCCTCCAGCTGCATCTCCGAGCATTGTGAATGCAAAAGCCTTGGGTAAT

Coding sequence (CDS)

ATGGCGAAGCCCGTTCAAATCGAAGTGTGGAACCCGAATGGGAAGTACAGGGTTGTGAGCACCAAGCCCATGCCTGGAACTCGCTGGATCAATCTCTTGATTGAGCAAGATTGCCGACTCGAAATTTGTACTGAGAAGAAGACGATTCTGTCTGTGGATGATATTCTCGCTCTCATCGGCGATAAGTGCGATGGCGTTATTGGACAGTTGACTGAAGATTGGGGAGAGGTGCTGTTTTCTGCACTGAGCAGAGCAGGAGGGAAAGCTTTTAGTAATATGGCTGTTGGTTACAATAATGTTGATGTTAATGCTGCTAATAAGTATGGTGTTGCTGTAGGAAACACTCCAGGAGTGCTTACAGAAACTACAGCAGAGTTGGCAGCTTCACTTTCTCTTGCAGCTGCAAGAAGGATAGTTGAAGCTGATGAGTTCATGAGGGCAGGTCTCTATGAGGGATGGCTTCCCAATTTGTTTGTTGGAAACTTGCTGAAAGGGCAGACTGTGGGTGTGATTGGAGCGGGTCGTATTGGATCTGCTTATGCAAGAATGATGGTAGAAGGATTCAAGATGAACTTAATCTACTATGATCTTTACCAGGCAACCAGACTCGAAAAGTTCGTTACAGCCTATGGTGAGTTCCTGGTAGCCAACGGTGAGGCACCCGTGTCATGGAAAAGAGCAACATCCATGGATGAGGTGCTCCGAGAGGCCGATGTGATAAGTCTTCATCCAGTACTCGATAAAACCACTTACCATCTGGTGAACAAAGAAAGTCTTAAAGCCATGAAGAAGGATGCAATCCTTATCAACTGTAGTAGGGGACCTGTCATCGACGAAGTAGCCCTTGTCGAGCATTTAAAAGAGAATCCAATGTTTCGAGTAGGCCTTGATGTCTTTGAGGATGAACCATACATGAAACCCGGGCTTGCTGATATGAAGAACGCGGTTATTGTTCCTCACATTGCTTCCGCTTCCAAGTGGACTCGTGAAGGAATGGCGACATTGGCTGCTCTTAATGTCCTGGGAACGATCAAAGATTATCCTGTTTGGGCCGATCCAAACCGGGTAGAACAGTTCCTTGACGAGAACATTTCCCCTCCAGCTGCATCTCCGAGCATTGTGAATGCAAAAGCCTTGGGTAAT

Protein sequence

MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIGDKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVISLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFEDEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFLDENISPPAASPSIVNAKALGN
Homology
BLAST of MC04g0389 vs. ExPASy Swiss-Prot
Match: P13443 (Glycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=HPR-A PE=2 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 8.4e-210
Identity = 360/381 (94.49%), Postives = 375/381 (98.43%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCR+EICTEKKTILSV+DILALIG
Sbjct: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRVEICTEKKTILSVEDILALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAG Y+GWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGRYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYGEFL ANGEAPV+W+RA+SMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGEFLKANGEAPVTWRRASSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTT+HLVNKESLKAMKKDAILINCSRGPVIDE ALV+HL++NPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTFHLVNKESLKAMKKDAILINCSRGPVIDEAALVDHLRDNPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNA+IVPHIASASKWTREGMATLAALNVLG IK YPVW+DPNRVE FL
Sbjct: 301 DEPYMKPGLADMKNAIIVPHIASASKWTREGMATLAALNVLGKIKGYPVWSDPNRVEPFL 360

Query: 361 DENISPPAASPSIVNAKALGN 382
           DEN+SPPAASPSIVNAKALGN
Sbjct: 361 DENVSPPAASPSIVNAKALGN 381

BLAST of MC04g0389 vs. ExPASy Swiss-Prot
Match: Q9C9W5 (Glycerate dehydrogenase HPR, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=HPR PE=1 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 7.6e-195
Identity = 332/380 (87.37%), Postives = 359/380 (94.47%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPV IEV+NPNGKYRVVSTKPMPGTRWINLL++Q CR+EIC  KKTILSV+DI+ LIG
Sbjct: 1   MAKPVSIEVYNPNGKYRVVSTKPMPGTRWINLLVDQGCRVEICHLKKTILSVEDIIDLIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGE LFSALS+AGGKAFSNMAVGYNNVDV AANKYG+AVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGETLFSALSKAGGKAFSNMAVGYNNVDVEAANKYGIAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMR GLYEGWLP+LFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRGGLYEGWLPHLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYG+FL ANGE PV+WKRA+SM+EVLREAD+I
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGQFLKANGEQPVTWKRASSMEEVLREADLI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTTYHLVNKE L  MKK+AIL+NCSRGPVIDE ALVEHLKENPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTYHLVNKERLAMMKKEAILVNCSRGPVIDEAALVEHLKENPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           +EP+MKPGLAD KNA++VPHIASASKWTREGMATLAALNVLG +K YP+W DPNRV+ FL
Sbjct: 301 EEPFMKPGLADTKNAIVVPHIASASKWTREGMATLAALNVLGRVKGYPIWHDPNRVDPFL 360

Query: 361 DENISPPAASPSIVNAKALG 381
           +EN SPP ASPSIVN+KALG
Sbjct: 361 NENASPPNASPSIVNSKALG 380

BLAST of MC04g0389 vs. ExPASy Swiss-Prot
Match: Q9C4M5 (Glyoxylate reductase OS=Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) OX=523849 GN=gyaR PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.0e-53
Identity = 129/302 (42.72%), Postives = 181/302 (59.93%), Query Frame = 0

Query: 49  ILSVDDILALIGDKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKY 108
           +  VD ++ L+ DK D    +L E+  ++          K  +  AVGY+N+D+  A K 
Sbjct: 43  VREVDALVTLVTDKVD---KELLENAPKL----------KIIAQYAVGYDNIDIEEATKR 102

Query: 109 GVAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRAGLYE----GWLPNLFVGNLLK 168
           G+ V NTPGVLT+ TA+LA +L LA ARRIVEAD F+R+G ++    GW P +F+G  LK
Sbjct: 103 GIYVTNTPGVLTDATADLAFALLLAVARRIVEADAFVRSGEWKKSEVGWHPLMFLGYGLK 162

Query: 169 GQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVTA-YGEFLVANGEAPVS 228
           G+T+G++G GRIG A A+   +GF M +IYY   +    E+ + A Y +F          
Sbjct: 163 GKTLGIVGFGRIGQALAK-RAKGFGMKIIYYSRTRKPEAEEEIGAEYVDF---------- 222

Query: 229 WKRATSMDEVLREADVISLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALV 288
                  + +L+E+D ISLH  L K TYH++ ++ LK MK +AILIN SRG V+D  AL+
Sbjct: 223 -------ETLLKESDFISLHVPLTKETYHMIGEKELKLMKPNAILINTSRGAVVDTNALI 282

Query: 289 EHLKENPMFRVGLDVFEDEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGT 346
           + LKE  +   GLDVFE+EPY    L  +KN V+ PHI SA+   REGMA L A N++  
Sbjct: 283 KALKEGWIAGAGLDVFEEEPYYNEELFKLKNVVLAPHIGSATHEAREGMAELVAKNLIAF 313

BLAST of MC04g0389 vs. ExPASy Swiss-Prot
Match: Q9UYR1 (Glyoxylate reductase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=gyaR PE=3 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.2e-51
Identity = 125/313 (39.94%), Postives = 184/313 (58.79%), Query Frame = 0

Query: 33  LIEQDCRLEICTEKKTILSVDDILALIGDKCDGVIGQLTEDWGEVLFSALSRAGGKAFSN 92
           ++E++  +E+  +++ I    +IL       D ++  L+E     +F    R   +  +N
Sbjct: 19  MLEKEFEVEVWEDEREI--PREILLEKVKDVDALVTMLSERIDREVFERAPRL--RIVAN 78

Query: 93  MAVGYNNVDVNAANKYGVAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRAGLYE- 152
            AVGY+N+DV  A K G+ V NTPGVLT+ TA+LA +L LA AR +V+ D+F R+G ++ 
Sbjct: 79  YAVGYDNIDVEEATKRGIYVTNTPGVLTDATADLAFALLLATARHLVKGDKFTRSGEWKK 138

Query: 153 ---GWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVT 212
               W P  F+G  + G+T+G+IG GRIG A A+    GF M ++YY   +   +EK + 
Sbjct: 139 RGVAWHPKWFLGYDVYGKTIGIIGFGRIGQAIAK-RARGFDMRILYYSRTRKPEVEKELN 198

Query: 213 AYGEFLVANGEAPVSWKRATSMDEVLREADVISLHPVLDKTTYHLVNKESLKAMKKDAIL 272
           A  EF                +DE+LRE+D + L   L+K TYH++N+E LK MK+ AIL
Sbjct: 199 A--EF--------------KPLDELLRESDFVVLAVPLNKETYHMINEERLKMMKRTAIL 258

Query: 273 INCSRGPVIDEVALVEHLKENPMFRVGLDVFEDEPYMKPGLADMKNAVIVPHIASASKWT 332
           IN +RG VID  AL++ LKE  +   GLDV+E+EPY    L  + N V+ PHI SA+   
Sbjct: 259 INVARGKVIDTKALIKALKEGWIAGAGLDVYEEEPYYNEELFSLDNVVLTPHIGSATFGA 310

Query: 333 REGMATLAALNVL 342
           REGMA L A N++
Sbjct: 319 REGMAKLVAENLI 310

BLAST of MC04g0389 vs. ExPASy Swiss-Prot
Match: O58320 (Glyoxylate reductase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=gyaR PE=1 SV=2)

HSP 1 Score: 202.6 bits (514), Expect = 8.0e-51
Identity = 123/313 (39.30%), Postives = 184/313 (58.79%), Query Frame = 0

Query: 33  LIEQDCRLEICTEKKTILSVDDILALIGDKCDGVIGQLTEDWGEVLFSALSRAGGKAFSN 92
           ++E +  +E+  ++K I    +IL     + D ++  L+E   + +F    +   +  +N
Sbjct: 18  MLEDEFEVEVWGDEKEI--PREILLKKVKEVDALVTMLSERIDKEVFENAPKL--RIVAN 77

Query: 93  MAVGYNNVDVNAANKYGVAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRAGLYE- 152
            AVGY+N+D+  A K G+ V NTP VLT+ TA+LA +L LA AR +V+ D F+R+G ++ 
Sbjct: 78  YAVGYDNIDIEEATKRGIYVTNTPDVLTDATADLAFALLLATARHVVKGDRFVRSGEWKK 137

Query: 153 ---GWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVT 212
               W P  F+G  + G+T+G+IG GRIG A A+   +GF M ++YY   +   +E+ + 
Sbjct: 138 RGVAWHPKWFLGYDVYGKTIGIIGLGRIGQAIAK-RAKGFNMRILYYSRTRKEEVERELN 197

Query: 213 AYGEFLVANGEAPVSWKRATSMDEVLREADVISLHPVLDKTTYHLVNKESLKAMKKDAIL 272
           A  EF                ++++LRE+D + L   L + TYHL+N+E LK MKK AIL
Sbjct: 198 A--EF--------------KPLEDLLRESDFVVLAVPLTRETYHLINEERLKLMKKTAIL 257

Query: 273 INCSRGPVIDEVALVEHLKENPMFRVGLDVFEDEPYMKPGLADMKNAVIVPHIASASKWT 332
           IN +RG V+D  ALV+ LKE  +   GLDVFE+EPY    L  + N V+ PHI SAS   
Sbjct: 258 INIARGKVVDTNALVKALKEGWIAGAGLDVFEEEPYYNEELFKLDNVVLTPHIGSASFGA 309

Query: 333 REGMATLAALNVL 342
           REGMA L A N++
Sbjct: 318 REGMAELVAKNLI 309

BLAST of MC04g0389 vs. NCBI nr
Match: XP_022135871.1 (glycerate dehydrogenase [Momordica charantia])

HSP 1 Score: 756 bits (1952), Expect = 2.64e-275
Identity = 379/380 (99.74%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSV+DILALIG
Sbjct: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVEDILALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL
Sbjct: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360

Query: 361 DENISPPAASPSIVNAKALG 380
           DENISPPAASPSIVNAKALG
Sbjct: 361 DENISPPAASPSIVNAKALG 380

BLAST of MC04g0389 vs. NCBI nr
Match: XP_038877465.1 (glycerate dehydrogenase isoform X2 [Benincasa hispida])

HSP 1 Score: 731 bits (1888), Expect = 1.29e-265
Identity = 361/381 (94.75%), Postives = 375/381 (98.43%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCR+EICTEKKTILSV+DI+ALIG
Sbjct: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRVEICTEKKTILSVEDIIALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAG Y+GWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGRYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYGEFL ANGEAPV+W+RA+SMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGEFLKANGEAPVTWRRASSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTT+HLVNKESLKAMKKDAILINCSRGPVIDE ALVEHLKENPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTFHLVNKESLKAMKKDAILINCSRGPVIDEAALVEHLKENPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNA++VPHIASASKWTREGMATLAALNVLG IK YPVW+DPNRVE FL
Sbjct: 301 DEPYMKPGLADMKNAIVVPHIASASKWTREGMATLAALNVLGKIKGYPVWSDPNRVEPFL 360

Query: 361 DENISPPAASPSIVNAKALGN 381
           DEN+SPPAASPSIVNAKALGN
Sbjct: 361 DENVSPPAASPSIVNAKALGN 381

BLAST of MC04g0389 vs. NCBI nr
Match: NP_001267567.1 (glycerate dehydrogenase [Cucumis sativus] >P13443.1 RecName: Full=Glycerate dehydrogenase; Short=GDH; AltName: Full=NADH-dependent hydroxypyruvate reductase; Short=HPR [Cucumis sativus] >KGN43256.1 hypothetical protein Csa_020156 [Cucumis sativus] >CAA32764.1 unnamed protein product [Cucumis sativus] >CAA41434.1 NADH-dependent hydroxypyruvate reductase [Cucumis sativus])

HSP 1 Score: 729 bits (1882), Expect = 1.06e-264
Identity = 360/381 (94.49%), Postives = 375/381 (98.43%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCR+EICTEKKTILSV+DILALIG
Sbjct: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRVEICTEKKTILSVEDILALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAG Y+GWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGRYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYGEFL ANGEAPV+W+RA+SMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGEFLKANGEAPVTWRRASSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTT+HLVNKESLKAMKKDAILINCSRGPVIDE ALV+HL++NPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTFHLVNKESLKAMKKDAILINCSRGPVIDEAALVDHLRDNPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNA+IVPHIASASKWTREGMATLAALNVLG IK YPVW+DPNRVE FL
Sbjct: 301 DEPYMKPGLADMKNAIIVPHIASASKWTREGMATLAALNVLGKIKGYPVWSDPNRVEPFL 360

Query: 361 DENISPPAASPSIVNAKALGN 381
           DEN+SPPAASPSIVNAKALGN
Sbjct: 361 DENVSPPAASPSIVNAKALGN 381

BLAST of MC04g0389 vs. NCBI nr
Match: XP_008447953.1 (PREDICTED: glycerate dehydrogenase isoform X2 [Cucumis melo] >KAA0049757.1 glycerate dehydrogenase isoform X2 [Cucumis melo var. makuwa] >TYK12121.1 glycerate dehydrogenase isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 729 bits (1882), Expect = 1.06e-264
Identity = 360/381 (94.49%), Postives = 375/381 (98.43%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLI+QDCR+EICTEKKTILSV+DILALIG
Sbjct: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIQQDCRVEICTEKKTILSVEDILALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAG Y+GWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGRYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYGEFL ANGEAPV+W+RA+SMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGEFLKANGEAPVTWRRASSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTT+HLVNKESLKAMKKDAILINCSRGPVIDE ALV+HL+ENPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTFHLVNKESLKAMKKDAILINCSRGPVIDEAALVDHLRENPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNA+IVPHIASASKWTREGMATLAALNVLG IK YPVW+DPNRVE FL
Sbjct: 301 DEPYMKPGLADMKNAIIVPHIASASKWTREGMATLAALNVLGKIKGYPVWSDPNRVEPFL 360

Query: 361 DENISPPAASPSIVNAKALGN 381
           DEN+SPPAASPSIVNAKALGN
Sbjct: 361 DENVSPPAASPSIVNAKALGN 381

BLAST of MC04g0389 vs. NCBI nr
Match: XP_038877464.1 (glycerate dehydrogenase isoform X1 [Benincasa hispida])

HSP 1 Score: 729 bits (1882), Expect = 1.24e-264
Identity = 360/380 (94.74%), Postives = 374/380 (98.42%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCR+EICTEKKTILSV+DI+ALIG
Sbjct: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRVEICTEKKTILSVEDIIALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAG Y+GWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGRYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYGEFL ANGEAPV+W+RA+SMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGEFLKANGEAPVTWRRASSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTT+HLVNKESLKAMKKDAILINCSRGPVIDE ALVEHLKENPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTFHLVNKESLKAMKKDAILINCSRGPVIDEAALVEHLKENPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNA++VPHIASASKWTREGMATLAALNVLG IK YPVW+DPNRVE FL
Sbjct: 301 DEPYMKPGLADMKNAIVVPHIASASKWTREGMATLAALNVLGKIKGYPVWSDPNRVEPFL 360

Query: 361 DENISPPAASPSIVNAKALG 380
           DEN+SPPAASPSIVNAKALG
Sbjct: 361 DENVSPPAASPSIVNAKALG 380

BLAST of MC04g0389 vs. ExPASy TrEMBL
Match: A0A6J1C1Y4 (glycerate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111007713 PE=3 SV=1)

HSP 1 Score: 756 bits (1952), Expect = 1.28e-275
Identity = 379/380 (99.74%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSV+DILALIG
Sbjct: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVEDILALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL
Sbjct: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360

Query: 361 DENISPPAASPSIVNAKALG 380
           DENISPPAASPSIVNAKALG
Sbjct: 361 DENISPPAASPSIVNAKALG 380

BLAST of MC04g0389 vs. ExPASy TrEMBL
Match: A0A5D3CJY4 (Glycerate dehydrogenase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00550 PE=3 SV=1)

HSP 1 Score: 729 bits (1882), Expect = 5.14e-265
Identity = 360/381 (94.49%), Postives = 375/381 (98.43%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLI+QDCR+EICTEKKTILSV+DILALIG
Sbjct: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIQQDCRVEICTEKKTILSVEDILALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAG Y+GWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGRYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYGEFL ANGEAPV+W+RA+SMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGEFLKANGEAPVTWRRASSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTT+HLVNKESLKAMKKDAILINCSRGPVIDE ALV+HL+ENPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTFHLVNKESLKAMKKDAILINCSRGPVIDEAALVDHLRENPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNA+IVPHIASASKWTREGMATLAALNVLG IK YPVW+DPNRVE FL
Sbjct: 301 DEPYMKPGLADMKNAIIVPHIASASKWTREGMATLAALNVLGKIKGYPVWSDPNRVEPFL 360

Query: 361 DENISPPAASPSIVNAKALGN 381
           DEN+SPPAASPSIVNAKALGN
Sbjct: 361 DENVSPPAASPSIVNAKALGN 381

BLAST of MC04g0389 vs. ExPASy TrEMBL
Match: A0A0A0K5Z2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G012940 PE=3 SV=1)

HSP 1 Score: 729 bits (1882), Expect = 5.14e-265
Identity = 360/381 (94.49%), Postives = 375/381 (98.43%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCR+EICTEKKTILSV+DILALIG
Sbjct: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRVEICTEKKTILSVEDILALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAG Y+GWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGRYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYGEFL ANGEAPV+W+RA+SMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGEFLKANGEAPVTWRRASSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTT+HLVNKESLKAMKKDAILINCSRGPVIDE ALV+HL++NPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTFHLVNKESLKAMKKDAILINCSRGPVIDEAALVDHLRDNPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNA+IVPHIASASKWTREGMATLAALNVLG IK YPVW+DPNRVE FL
Sbjct: 301 DEPYMKPGLADMKNAIIVPHIASASKWTREGMATLAALNVLGKIKGYPVWSDPNRVEPFL 360

Query: 361 DENISPPAASPSIVNAKALGN 381
           DEN+SPPAASPSIVNAKALGN
Sbjct: 361 DENVSPPAASPSIVNAKALGN 381

BLAST of MC04g0389 vs. ExPASy TrEMBL
Match: A0A1S3BJ84 (glycerate dehydrogenase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490283 PE=3 SV=1)

HSP 1 Score: 729 bits (1882), Expect = 5.14e-265
Identity = 360/381 (94.49%), Postives = 375/381 (98.43%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLI+QDCR+EICTEKKTILSV+DILALIG
Sbjct: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIQQDCRVEICTEKKTILSVEDILALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAG Y+GWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGRYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYGEFL ANGEAPV+W+RA+SMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGEFLKANGEAPVTWRRASSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTT+HLVNKESLKAMKKDAILINCSRGPVIDE ALV+HL+ENPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTFHLVNKESLKAMKKDAILINCSRGPVIDEAALVDHLRENPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNA+IVPHIASASKWTREGMATLAALNVLG IK YPVW+DPNRVE FL
Sbjct: 301 DEPYMKPGLADMKNAIIVPHIASASKWTREGMATLAALNVLGKIKGYPVWSDPNRVEPFL 360

Query: 361 DENISPPAASPSIVNAKALGN 381
           DEN+SPPAASPSIVNAKALGN
Sbjct: 361 DENVSPPAASPSIVNAKALGN 381

BLAST of MC04g0389 vs. ExPASy TrEMBL
Match: A0A6J1HXX7 (glycerate dehydrogenase isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111468468 PE=3 SV=1)

HSP 1 Score: 724 bits (1870), Expect = 3.34e-263
Identity = 358/380 (94.21%), Postives = 371/380 (97.63%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPVQ+EVWNPNGKYRVVSTKPMPGTRWINLLIEQDCR+EICTEKKTILSV+DI+ALIG
Sbjct: 1   MAKPVQVEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRVEICTEKKTILSVEDIVALIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYG+AVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGIAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMRAG Y+GWLPNLFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRAGHYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYGEFL ANGE PV+W+RA+SMDEVLREADVI
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGEFLKANGEVPVTWRRASSMDEVLREADVI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTT+HLVNKESLKAMKKDAILINCSRGPVIDE ALVEHLKENPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTFHLVNKESLKAMKKDAILINCSRGPVIDEAALVEHLKENPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           DEPYMKPGLADMKNA+IVPHIASASKWTREGMATLAALNVLG IK YPVWADPNRVE FL
Sbjct: 301 DEPYMKPGLADMKNAIIVPHIASASKWTREGMATLAALNVLGKIKQYPVWADPNRVEPFL 360

Query: 361 DENISPPAASPSIVNAKALG 380
           DEN  PPAASPSIVNAKALG
Sbjct: 361 DENAPPPAASPSIVNAKALG 380

BLAST of MC04g0389 vs. TAIR 10
Match: AT1G68010.1 (hydroxypyruvate reductase )

HSP 1 Score: 681.0 bits (1756), Expect = 5.4e-196
Identity = 332/380 (87.37%), Postives = 359/380 (94.47%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPV IEV+NPNGKYRVVSTKPMPGTRWINLL++Q CR+EIC  KKTILSV+DI+ LIG
Sbjct: 1   MAKPVSIEVYNPNGKYRVVSTKPMPGTRWINLLVDQGCRVEICHLKKTILSVEDIIDLIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGE LFSALS+AGGKAFSNMAVGYNNVDV AANKYG+AVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGETLFSALSKAGGKAFSNMAVGYNNVDVEAANKYGIAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMR GLYEGWLP+LFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRGGLYEGWLPHLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYG+FL ANGE PV+WKRA+SM+EVLREAD+I
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGQFLKANGEQPVTWKRASSMEEVLREADLI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFE 300
           SLHPVLDKTTYHLVNKE L  MKK+AIL+NCSRGPVIDE ALVEHLKENPMFRVGLDVFE
Sbjct: 241 SLHPVLDKTTYHLVNKERLAMMKKEAILVNCSRGPVIDEAALVEHLKENPMFRVGLDVFE 300

Query: 301 DEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQFL 360
           +EP+MKPGLAD KNA++VPHIASASKWTREGMATLAALNVLG +K YP+W DPNRV+ FL
Sbjct: 301 EEPFMKPGLADTKNAIVVPHIASASKWTREGMATLAALNVLGRVKGYPIWHDPNRVDPFL 360

Query: 361 DENISPPAASPSIVNAKALG 381
           +EN SPP ASPSIVN+KALG
Sbjct: 361 NENASPPNASPSIVNSKALG 380

BLAST of MC04g0389 vs. TAIR 10
Match: AT1G68010.2 (hydroxypyruvate reductase )

HSP 1 Score: 676.4 bits (1744), Expect = 1.3e-194
Identity = 332/381 (87.14%), Postives = 359/381 (94.23%), Query Frame = 0

Query: 1   MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRLEICTEKKTILSVDDILALIG 60
           MAKPV IEV+NPNGKYRVVSTKPMPGTRWINLL++Q CR+EIC  KKTILSV+DI+ LIG
Sbjct: 1   MAKPVSIEVYNPNGKYRVVSTKPMPGTRWINLLVDQGCRVEICHLKKTILSVEDIIDLIG 60

Query: 61  DKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLT 120
           DKCDGVIGQLTEDWGE LFSALS+AGGKAFSNMAVGYNNVDV AANKYG+AVGNTPGVLT
Sbjct: 61  DKCDGVIGQLTEDWGETLFSALSKAGGKAFSNMAVGYNNVDVEAANKYGIAVGNTPGVLT 120

Query: 121 ETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAY 180
           ETTAELAASLSLAAARRIVEADEFMR GLYEGWLP+LFVGNLLKGQTVGVIGAGRIGSAY
Sbjct: 121 ETTAELAASLSLAAARRIVEADEFMRGGLYEGWLPHLFVGNLLKGQTVGVIGAGRIGSAY 180

Query: 181 ARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVI 240
           ARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYG+FL ANGE PV+WKRA+SM+EVLREAD+I
Sbjct: 181 ARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGQFLKANGEQPVTWKRASSMEEVLREADLI 240

Query: 241 SLHPVLDKTTYHLVNKESLKAMKK-DAILINCSRGPVIDEVALVEHLKENPMFRVGLDVF 300
           SLHPVLDKTTYHLVNKE L  MKK +AIL+NCSRGPVIDE ALVEHLKENPMFRVGLDVF
Sbjct: 241 SLHPVLDKTTYHLVNKERLAMMKKVEAILVNCSRGPVIDEAALVEHLKENPMFRVGLDVF 300

Query: 301 EDEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNVLGTIKDYPVWADPNRVEQF 360
           E+EP+MKPGLAD KNA++VPHIASASKWTREGMATLAALNVLG +K YP+W DPNRV+ F
Sbjct: 301 EEEPFMKPGLADTKNAIVVPHIASASKWTREGMATLAALNVLGRVKGYPIWHDPNRVDPF 360

Query: 361 LDENISPPAASPSIVNAKALG 381
           L+EN SPP ASPSIVN+KALG
Sbjct: 361 LNENASPPNASPSIVNSKALG 381

BLAST of MC04g0389 vs. TAIR 10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 132.9 bits (333), Expect = 5.5e-31
Identity = 90/299 (30.10%), Postives = 149/299 (49.83%), Query Frame = 0

Query: 45  EKKTILSV--DDILALIGDKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDV 104
           EK  +L    + I A++G+   G   QL  D   +          +  S+ +VG + +D+
Sbjct: 34  EKSVLLETHRNSIRAVVGNASAGADAQLISDLPNL----------EIVSSFSVGLDKIDL 93

Query: 105 NAANKYGVAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLF-VGN 164
               + G+ V NTP VLTE  A+LA  L LA  RR+ E D ++R+G    W    F +  
Sbjct: 94  GKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDRYVRSG---KWKQGEFQLTT 153

Query: 165 LLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAP 224
              G++VG+IG GRIG+A A+   E F   + YY                       +  
Sbjct: 154 KFSGKSVGIIGLGRIGTAIAK-RAEAFSCPINYYS-------------------RTIKPD 213

Query: 225 VSWKRATSMDEVLREADVISLHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVA 284
           V++K   ++ ++ + +D++ +   L + T H+V+++ + A+    +LIN  RGP +DE  
Sbjct: 214 VAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQE 273

Query: 285 LVEHLKENPMFRVGLDVFEDEPYMKPGLADMKNAVIVPHIASASKWTREGMATLAALNV 341
           L++ L E  +    LDVFE EP++   L  ++N V++PH+ S +  TR  MA L   N+
Sbjct: 274 LIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNL 299

BLAST of MC04g0389 vs. TAIR 10
Match: AT1G17745.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 130.6 bits (327), Expect = 2.7e-30
Identity = 111/343 (32.36%), Postives = 163/343 (47.52%), Query Frame = 0

Query: 10  WNPN-GKYRVVSTKPMPGTRWINLLIE---QDCRLEICTE--KKTILSVDDILALIGDKC 69
           +NP   K R++ T+ + G   +NLL E    DC  ++  E  KK +   D ++   G K 
Sbjct: 76  YNPTLPKPRILVTEKL-GEAGVNLLREFGDVDCSYDLSPEDLKKKVAESDALIVRSGTKV 135

Query: 70  DGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLTETT 129
                       EV  +A  R   K      VG +NVD+ AA ++G  V N P   T   
Sbjct: 136 T----------REVFEAAKGRL--KVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAA 195

Query: 130 AELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAYARM 189
           AE   +L  + AR + +AD  ++AG    W  + +VG  L G+T+ V+G G++G+  AR 
Sbjct: 196 AEHGIALLASMARNVAQADASIKAG---KWERSKYVGVSLVGKTLAVMGFGKVGTEVAR- 255

Query: 190 MVEGFKMNLIYYDLYQATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVISLH 249
             +G  M +I +D Y      +   A G  LV             S D+ +  AD +SLH
Sbjct: 256 RAKGLGMTVISHDPYAPADRAR---ALGVDLV-------------SFDQAISTADFVSLH 315

Query: 250 PVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFEDEP 309
             L   T  + N E+   MKK   LIN +RG VIDE ALV  L    + +  LDVF +EP
Sbjct: 316 MPLTPATKKVFNDETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEP 375

Query: 310 YMKPG-LADMKNAVIVPHIASASKWTREGMATLAALNVLGTIK 346
             K   L   +N  + PH+ +++K  +EG+A   A  V G +K
Sbjct: 376 PSKDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVAGALK 385

BLAST of MC04g0389 vs. TAIR 10
Match: AT4G34200.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 128.3 bits (321), Expect = 1.4e-29
Identity = 98/284 (34.51%), Postives = 140/284 (49.30%), Query Frame = 0

Query: 63  CDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLTET 122
           CD +I +     G  +F + S    K      VG +NVD++AA ++G  V N P   T  
Sbjct: 102 CDALIVRSGTKVGREVFES-SHGRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIA 161

Query: 123 TAELAASLSLAAARRIVEADEFMRAGLYEGWLPNLFVGNLLKGQTVGVIGAGRIGSAYAR 182
            AE   +L  A AR + +AD  ++AG    W  N +VG  L G+T+ V+G G++G+  AR
Sbjct: 162 AAEHGIALMAAMARNVAQADASVKAG---EWKRNKYVGVSLVGKTLAVLGFGKVGTEVAR 221

Query: 183 MMVEGFKMNLIYYDLY-QATRLEKFVTAYGEFLVANGEAPVSWKRATSMDEVLREADVIS 242
              +G  M +I +D Y  A R      A G  LV             S DE L  AD IS
Sbjct: 222 -RAKGLGMRVIAHDPYAPADRAH----AIGVDLV-------------SFDEALATADFIS 281

Query: 243 LHPVLDKTTYHLVNKESLKAMKKDAILINCSRGPVIDEVALVEHLKENPMFRVGLDVFED 302
           LH  L  TT  ++N E+   MKK   ++N +RG VIDE ALV  L    + +  LDVF  
Sbjct: 282 LHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDALVRALDAGIVAQAALDVFTK 341

Query: 303 EPYMKPG-LADMKNAVIVPHIASASKWTREGMATLAALNVLGTI 345
           EP  K   L   +   + PH+ +++   +EG+A   A  V+G +
Sbjct: 342 EPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVVGAL 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P134438.4e-21094.49Glycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=HPR-A PE=2 SV=1[more]
Q9C9W57.6e-19587.37Glycerate dehydrogenase HPR, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=HPR ... [more]
Q9C4M51.0e-5342.72Glyoxylate reductase OS=Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / J... [more]
Q9UYR11.2e-5139.94Glyoxylate reductase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=gyaR... [more]
O583208.0e-5139.30Glyoxylate reductase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / ... [more]
Match NameE-valueIdentityDescription
XP_022135871.12.64e-27599.74glycerate dehydrogenase [Momordica charantia][more]
XP_038877465.11.29e-26594.75glycerate dehydrogenase isoform X2 [Benincasa hispida][more]
NP_001267567.11.06e-26494.49glycerate dehydrogenase [Cucumis sativus] >P13443.1 RecName: Full=Glycerate dehy... [more]
XP_008447953.11.06e-26494.49PREDICTED: glycerate dehydrogenase isoform X2 [Cucumis melo] >KAA0049757.1 glyce... [more]
XP_038877464.11.24e-26494.74glycerate dehydrogenase isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1C1Y41.28e-27599.74glycerate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111007713 PE=3 SV=1[more]
A0A5D3CJY45.14e-26594.49Glycerate dehydrogenase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0K5Z25.14e-26594.49Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G012940 PE=3 SV=1[more]
A0A1S3BJ845.14e-26594.49glycerate dehydrogenase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490283 PE=3 ... [more]
A0A6J1HXX73.34e-26394.21glycerate dehydrogenase isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111468468 P... [more]
Match NameE-valueIdentityDescription
AT1G68010.15.4e-19687.37hydroxypyruvate reductase [more]
AT1G68010.21.3e-19487.14hydroxypyruvate reductase [more]
AT1G79870.15.5e-3130.10D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G17745.12.7e-3032.36D-3-phosphoglycerate dehydrogenase [more]
AT4G34200.11.4e-2934.51D-3-phosphoglycerate dehydrogenase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 130..322
e-value: 1.2E-46
score: 158.3
NoneNo IPR availableGENE3D3.40.50.720coord: 118..321
e-value: 2.8E-98
score: 330.7
NoneNo IPR availableGENE3D3.40.50.720coord: 18..345
e-value: 2.8E-98
score: 330.7
NoneNo IPR availablePANTHERPTHR10996:SF257ZGC:136493coord: 28..347
NoneNo IPR availablePANTHERPTHR109962-HYDROXYACID DEHYDROGENASE-RELATEDcoord: 28..347
NoneNo IPR availableCDDcd05301GDHcoord: 16..341
e-value: 3.13618E-120
score: 348.615
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 17..156
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 35..345
e-value: 5.0E-16
score: 58.9
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00671D_2_HYDROXYACID_DH_3coord: 262..278
IPR029752D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1PROSITEPS00065D_2_HYDROXYACID_DH_1coord: 168..196
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 120..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0389.1MC04g0389.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009854 oxidative photosynthetic carbon pathway
cellular_component GO:0005829 cytosol
cellular_component GO:0005777 peroxisome
molecular_function GO:0008465 glycerate dehydrogenase activity
molecular_function GO:0030267 glyoxylate reductase (NADP+) activity
molecular_function GO:0016618 hydroxypyruvate reductase activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor