MC04g0369 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0369
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDDB1- and CUL4-associated factor 8
LocationMC04: 2956691 .. 2960033 (+)
RNA-Seq ExpressionMC04g0369
SyntenyMC04g0369
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCACGTGCCTATCAATATAAACACTATTATAAAAGTTAGGGACAAACTTGGAAATTTGTAATTACGGATAAAAAAGAAATTAAGAAAAAAAAGAGGAGAAAAGCGAAAAATGTATTGGACGTGCCTTAATCATTGATAATCGTAAATAAGAAAGAGCGGTGAGATAAGCTCAAGAGGAAGATTGATTTGATGGTTGTGTGTGTGAGCGACTGCGATGCTCTGCTTCTGCTTAAAGCGGTGAACGCCGAAATAACTGTTCTGTTCTGTTCATCTTCATAGGCTAACAATTAACAAAATCCAGCGAATAGCGATGCCCTTCTTGATTCCGTAACGCCCTCTCTCTGCCCGCTTCCCTGATTTTGATCCCATCGAACTCAGATTCGTTCATCTTCGATTCCACAATGAACAAGAGGGCCAGAATTGATGATTCCAATAAGGCACTCGTCAATGTCTGGAAGCGGGAGGTCGGCGAGCTTTCCACCAGGAATTTCGCTCACCGTCTTGGTGCCTCCCAGGTATTGCTCCCGGATTTTCCCTTATTACTTTTTGTTTTATCAATAATTTTTTCTGTGTTTATAAATGATCCTCTCAGGTTAGTTGTTCTGATTTTTAAATGTCATCGTTTGTTGTTCGATCACAACCGAATGGACCCAGTAGAAAGAATCGGATTGTGTTGCATTTTTCGAAAGGATTTTGTTTTGATTAAAAAAAATTATACGGGTTTCGGTGGATAGTCTACTAGCTTGAAGCATTTTTGTACTTGTTTCTGGTATTGGGTGGCTTCTAGTATCTGTGCTTTTAAAGGTGTTTTAATGTTTCGTTTCATTAGCTGAGGATCGCTTGTGTTACTGCTATTTCAGGATCTTGTGCTGCGACTTGATATTTACAGGAAATTGGAGAGGCACAAAGGTTGTGTCAACACTGTGAGCTTTAATGCTGCTGGTGACGTTCTAGTTTCGGGTTCTGATGATCGGCGGGTAATCCTCTGGAATTGGGAGACCGGGCAAGTCAAGCTCTCTTTTCAGTCTGGTCATAATAACAATGTTTTTCAAGCAAAAATCATGCCTTACACGGATGACCGATGCATTGTGACTTGTGCTGCGGATGGGCAGGTATGTCATCTAGTTGAAGGGCAGGATTTTTCTAGACAATATGGCAAAGAATTAGACCCTTGTGGTCTAACATGGTTCTTATTTAGTTTTCAAGTCAAGAGCTCTTGTTCCCCCTCCCTCCCTCCCTCCTTATTCATTTAGGATGCTGTACATTTAGTTGAAAATCATCAAAGCTCTTGTAATTATCATTAAAAATTAATATTGTTTCTCTATAGTCAAAGGGACCATAAAAGTGCTTCGGTTGGACCTTTCCACAATTATTTAGCGTTGCTTTTGAGCCGCCACCTGAGGCGATTCTAGGGGCCAATCTGCAATGTCATTAGGGAAACAACAAATTAGTACAAAAGTTTTTGAAAGGGATAACCACCTAGCCACAAACTTATGATGAATAAGGAGGTGATTCGTAGTCTCTTCATCCTTTTTACATTTTCGGGGAAAATCTGAGGCTAGTACAAAGTAAGTGATTCCTTATTTGAGTTCTATTTGATTGTTGAGACTAGCTTTAGTCTTCTTTTTGTTCCACCTTTCAAGTTTACACATTGAAACTTCCTAATAGTGACCGTTCTTTATTGAAGCCTTTGATGAGACACCAGATTTGTTAGGGTGAAAAAGATTGATAATATAAGTTTTTTGCAGGTGTCTTTGTCTATTATCAAGAAATCTTCCCTTGTTATCGCTGTAGTAACATCTTGTGATGATTATCACTTTCTGTTTATTTCTCCGATCTCCTGTCGGCATAGAGACTCTCCTTTGACCTTTCCGATCAATTTTGCTGTTGTTTACTTCTCTTTCCTGCCTAATAAAGTTTTCTTCACCGGAGCTCTTTTTTCTTCCCCTTCAAAGAATCTTTTAATCAGCCCAAAAAATTCCTCTGGATCTGAATTATTTTCTCTCTGGGATCACTATTCAATCTCTTTCCACTACTTTGAATGTAAGTAATCTTAATTAAATCCTGTGTCTTATTGAAATTTGCTGATCCAAATGAAGCCATTTTCACAGTCAATCTTCCAAAAGCAACTTTACATTAAAAGGGGGGTTTAAAAGGAGGGTTTCCAGGCATTTGCTATCCAAATTGCTTTTCTTCATTCCACCGATATCTCATCAATGTCTTAGCAATTATAAATTATCTCATCAGTAGCCACCTCTACGCTTCATTGTTGCTGATGAGACTATTTCCTTATTAAAATCACAGAAGTGAAGTTGATTTTTGTGTATATGGTATTTTTGTTGTCGGTCCTCTGTACATATCATAGCCAATCATCAAGTGGGAGGATCAAGCAATATGCTAGATGGAAATATATGTGTTTTTCCCCCAATTTTGTTCTTTACTGGCATTGATTAATAAATTAATGTTTAAAAACGAGTTGCATTTTTTTCTCAATTCAAGCAAAACTGCCTGTTTATTTTCTGTCGGCAGAATGTTTCCTTTGTTTTCTCCCACTAGAAAGCTTGTTTATCTGTGCTATGTACCAAAAACATTCATTCCTTAATAAGAGTATTATTGGCTGAAATTCAGGTGAGACATGCTCAGATTTTGAATAGTGGGCGTGTGGAAACTTCATTACTTGGCAGTCATCAAGGGCGAGCTCATAAATTGGCCATTGAACCTGGGAGCCCTTATATTTTTTATACTTGTGGTGAAGATGGATTGGTGCAACATGTGAGTTTTTATCGTTCATTCTGTTGTTATTTTAAGTTCCATCAACATTCCAAGTATTACTGCAACTTGATAAGTTATATGCTTATTCATGGCCAAAGTACATGACATTTTTTGAAGATTTGTAACTAAACTTAAAGCATGAAATTATATTTTTTTTCCAAAGAACATTCTTCTTTAATATCGCTGAATTGCAGTGTGATACTGTATTTTTCCTTTTAATAGTTTGATCTGAGAACTGGGGCTGCCAGTGAGCTGTTCACTTGCCAATCAATTGACAATAGGGCAGGTTACATGTCATCGATTCAGCTAAATGCAATTACTATTGATCCAAGAAATCCAAATCTCTTTGTTGTTGCTGGTTCAGATGAGTACGCTCGGTTGTATGATATTCGCAAGTACAAGGGGGAAGGATCAACCGATTTTGGTCAACCTACAGACTACTATTGCCCCCCTCACTTGATTGGTGATGAACAAGTGGGAATAACAGGTTTAGCCTACTCAGAGCTGAGCGAACTTCTTGTCTCATACAATGATGAATCCATTTATCTCTTTAATAGGGATATGGGTTTG

mRNA sequence

GTCACGTGCCTATCAATATAAACACTATTATAAAAGTTAGGGACAAACTTGGAAATTTGTAATTACGGATAAAAAAGAAATTAAGAAAAAAAAGAGGAGAAAAGCGAAAAATGTATTGGACGTGCCTTAATCATTGATAATCGTAAATAAGAAAGAGCGGTGAGATAAGCTCAAGAGGAAGATTGATTTGATGGTTGTGTGTGTGAGCGACTGCGATGCTCTGCTTCTGCTTAAAGCGGTGAACGCCGAAATAACTGTTCTGTTCTGTTCATCTTCATAGGCTAACAATTAACAAAATCCAGCGAATAGCGATGCCCTTCTTGATTCCGTAACGCCCTCTCTCTGCCCGCTTCCCTGATTTTGATCCCATCGAACTCAGATTCGTTCATCTTCGATTCCACAATGAACAAGAGGGCCAGAATTGATGATTCCAATAAGGCACTCGTCAATGTCTGGAAGCGGGAGGTCGGCGAGCTTTCCACCAGGAATTTCGCTCACCGTCTTGGTGCCTCCCAGGATCTTGTGCTGCGACTTGATATTTACAGGAAATTGGAGAGGCACAAAGGTTGTGTCAACACTGTGAGCTTTAATGCTGCTGGTGACGTTCTAGTTTCGGGTTCTGATGATCGGCGGGTAATCCTCTGGAATTGGGAGACCGGGCAAGTCAAGCTCTCTTTTCAGTCTGGTCATAATAACAATGTTTTTCAAGCAAAAATCATGCCTTACACGGATGACCGATGCATTGTGACTTGTGCTGCGGATGGGCAGGTGAGACATGCTCAGATTTTGAATAGTGGGCGTGTGGAAACTTCATTACTTGGCAGTCATCAAGGGCGAGCTCATAAATTGGCCATTGAACCTGGGAGCCCTTATATTTTTTATACTTGTGGTGAAGATGGATTGGTGCAACATGTGAGTTTTTATCGTTCATTCTGTTGTTATTTTAAGTTCCATCAACATTCCAATGAGCTGTTCACTTGCCAATCAATTGACAATAGGGCAGGTTACATGTCATCGATTCAGCTAAATGCAATTACTATTGATCCAAGAAATCCAAATCTCTTTGTTGTTGCTGGTTCAGATGAGTACGCTCGGTTGTATGATATTCGCAAGTACAAGGGGGAAGGATCAACCGATTTTGGTCAACCTACAGACTACTATTGCCCCCCTCACTTGATTGGTGATGAACAAGTGGGAATAACAGGTTTAGCCTACTCAGAGCTGAGCGAACTTCTTGTCTCATACAATGATGAATCCATTTATCTCTTTAATAGGGATATGGGTTTG

Coding sequence (CDS)

ATGAACAAGAGGGCCAGAATTGATGATTCCAATAAGGCACTCGTCAATGTCTGGAAGCGGGAGGTCGGCGAGCTTTCCACCAGGAATTTCGCTCACCGTCTTGGTGCCTCCCAGGATCTTGTGCTGCGACTTGATATTTACAGGAAATTGGAGAGGCACAAAGGTTGTGTCAACACTGTGAGCTTTAATGCTGCTGGTGACGTTCTAGTTTCGGGTTCTGATGATCGGCGGGTAATCCTCTGGAATTGGGAGACCGGGCAAGTCAAGCTCTCTTTTCAGTCTGGTCATAATAACAATGTTTTTCAAGCAAAAATCATGCCTTACACGGATGACCGATGCATTGTGACTTGTGCTGCGGATGGGCAGGTGAGACATGCTCAGATTTTGAATAGTGGGCGTGTGGAAACTTCATTACTTGGCAGTCATCAAGGGCGAGCTCATAAATTGGCCATTGAACCTGGGAGCCCTTATATTTTTTATACTTGTGGTGAAGATGGATTGGTGCAACATGTGAGTTTTTATCGTTCATTCTGTTGTTATTTTAAGTTCCATCAACATTCCAATGAGCTGTTCACTTGCCAATCAATTGACAATAGGGCAGGTTACATGTCATCGATTCAGCTAAATGCAATTACTATTGATCCAAGAAATCCAAATCTCTTTGTTGTTGCTGGTTCAGATGAGTACGCTCGGTTGTATGATATTCGCAAGTACAAGGGGGAAGGATCAACCGATTTTGGTCAACCTACAGACTACTATTGCCCCCCTCACTTGATTGGTGATGAACAAGTGGGAATAACAGGTTTAGCCTACTCAGAGCTGAGCGAACTTCTTGTCTCATACAATGATGAATCCATTTATCTCTTTAATAGGGATATGGGTTTG

Protein sequence

MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTVSFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAADGQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCYFKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL
Homology
BLAST of MC04g0369 vs. ExPASy Swiss-Prot
Match: Q8N7N5 (DDB1- and CUL4-associated factor 8 OS=Mus musculus OX=10090 GN=Dcaf8 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.1e-45
Identity = 107/282 (37.94%), Postives = 155/282 (54.96%), Query Frame = 0

Query: 12  KALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTVSFNAAGDVLVS 71
           +AL  + +RE+G  S+  F +    ++  V R  +   LE H GCVNT+ FN  G  L S
Sbjct: 147 QALPALRERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLAS 206

Query: 72  GSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAADGQVRHAQILNS 131
           GSDD +V++W+W   Q  L F+SGH +NVFQAK +P + D  +  CA DGQVR A++  +
Sbjct: 207 GSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSAT 266

Query: 132 GRVE-TSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCYFKFHQHSNEL 191
              + T  +  H+G +HKLA+EP SP  F + GED +V        F    +  + +++L
Sbjct: 267 QCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVV--------FTIDLRQDRPASKL 326

Query: 192 FTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPT 251
              +  + + G      L  I ++P N + F V G D+Y R+YD RK       +     
Sbjct: 327 VVTKEKEKKVG------LYTIYVNPANTHQFAVGGRDQYVRIYDQRKI---DENENNGVL 386

Query: 252 DYYCPPHLIGDE-QVGITGLAYS-ELSELLVSYNDESIYLFN 291
             +CP HL+  E +  IT L YS + +ELL SYNDE IYLFN
Sbjct: 387 KKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFN 409

BLAST of MC04g0369 vs. ExPASy Swiss-Prot
Match: Q5TAQ9 (DDB1- and CUL4-associated factor 8 OS=Homo sapiens OX=9606 GN=DCAF8 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.5e-44
Identity = 106/282 (37.59%), Postives = 155/282 (54.96%), Query Frame = 0

Query: 12  KALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTVSFNAAGDVLVS 71
           +AL  + +RE+G  S+  F +    ++  V R  +   LE H GCVNT+ FN  G  L S
Sbjct: 153 QALPALRERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLAS 212

Query: 72  GSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAADGQVRHAQILNS 131
           GSDD +V++W+W   Q  L F+SGH +NVFQAK +P + D  +  CA DGQVR A++  +
Sbjct: 213 GSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSAT 272

Query: 132 GRVE-TSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCYFKFHQHSNEL 191
              + T  +  H+G +HKLA+EP SP  F + GED +V        F    +  + +++L
Sbjct: 273 QCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVV--------FTIDLRQDRPASKL 332

Query: 192 FTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPT 251
              +  + + G      L  I ++P N + F V G D++ R+YD RK       +     
Sbjct: 333 VVTKEKEKKVG------LYTIYVNPANTHQFAVGGRDQFVRIYDQRKI---DENENNGVL 392

Query: 252 DYYCPPHLIGDE-QVGITGLAYS-ELSELLVSYNDESIYLFN 291
             +CP HL+  E +  IT L YS + +ELL SYNDE IYLFN
Sbjct: 393 KKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFN 415

BLAST of MC04g0369 vs. ExPASy Swiss-Prot
Match: Q5U2M6 (DDB1- and CUL4-associated factor 8 OS=Rattus norvegicus OX=10116 GN=Dcaf8 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.5e-44
Identity = 106/282 (37.59%), Postives = 155/282 (54.96%), Query Frame = 0

Query: 12  KALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTVSFNAAGDVLVS 71
           +AL  + +RE+G  S+  F +    ++  V R  +   LE H GCVNT+ FN  G  L S
Sbjct: 147 QALPALRERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLAS 206

Query: 72  GSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAADGQVRHAQILNS 131
           GSDD +V++W+W   Q  L F+SGH +NVFQAK +P + D  +  CA DGQVR A++  +
Sbjct: 207 GSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSAT 266

Query: 132 GRVE-TSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCYFKFHQHSNEL 191
              + T  +  H+G +HKLA+EP SP  F + GED +V        F    +  + +++L
Sbjct: 267 QCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVV--------FTIDLRQDRPASKL 326

Query: 192 FTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPT 251
              +  + + G      L  I ++P N + F V G D++ R+YD RK       +     
Sbjct: 327 VVTKEKEKKVG------LYTIYVNPANTHQFAVGGRDQFVRIYDQRKI---DENENNGVL 386

Query: 252 DYYCPPHLIGDE-QVGITGLAYS-ELSELLVSYNDESIYLFN 291
             +CP HL+  E +  IT L YS + +ELL SYNDE IYLFN
Sbjct: 387 KKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFN 409

BLAST of MC04g0369 vs. ExPASy Swiss-Prot
Match: P0C7V8 (DDB1- and CUL4-associated factor 8-like protein 2 OS=Homo sapiens OX=9606 GN=DCAF8L2 PE=2 SV=2)

HSP 1 Score: 179.5 bits (454), Expect = 5.6e-44
Identity = 104/275 (37.82%), Postives = 149/275 (54.18%), Query Frame = 0

Query: 19  KREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTVSFNAAGDVLVSGSDDRRV 78
           +R++G  S   F +    ++  V R  +  +L  H GCVNTV FN  G  L S  DD +V
Sbjct: 195 QRQLG--SRPRFVYEACGARAFVQRFRLQYRLADHVGCVNTVHFNQRGTRLASSGDDLKV 254

Query: 79  ILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAADGQVRHAQILNSGRV-ETS 138
           I+W+W   +  L+F+SGH NNVFQAK +P   D  +  CA DGQVR A+++N+     T 
Sbjct: 255 IVWDWVRQRPVLNFESGHTNNVFQAKFLPNCGDSTLAMCARDGQVRVAELINASYFNNTK 314

Query: 139 LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCYFKFHQHSNELFTCQSID 198
            +  H+G AHKLA+EP SPY F T GED +V        F    +  + ++++   +  D
Sbjct: 315 CVAQHRGPAHKLALEPDSPYKFLTSGEDAVV--------FTIDLRQDRPASKVVVTREND 374

Query: 199 NRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPTDYYCPPH 258
            + G      L  IT++P N   F V G D++ R+YD RK   + +         + P H
Sbjct: 375 KKVG------LYTITVNPANTYQFAVGGQDQFVRIYDQRKIDKKENNGV---LKKFTPHH 434

Query: 259 LIG-DEQVGITGLAYS-ELSELLVSYNDESIYLFN 291
           L+  D    IT + YS + +ELL SYND+ IYLFN
Sbjct: 435 LVNCDFPTNITCVVYSHDGTELLASYNDDDIYLFN 450

BLAST of MC04g0369 vs. ExPASy Swiss-Prot
Match: Q6NRH1 (DDB1- and CUL4-associated factor 8 OS=Xenopus laevis OX=8355 GN=dcaf8 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 5.6e-44
Identity = 104/282 (36.88%), Postives = 154/282 (54.61%), Query Frame = 0

Query: 12  KALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTVSFNAAGDVLVS 71
           +AL  + +R++G  S+  F +    ++  V R  +   L+ H GCVNT+ FN  G  L S
Sbjct: 156 RALSALRQRQMG--SSTRFIYEACGARGFVQRFHLLHGLDGHSGCVNTLHFNQRGTCLAS 215

Query: 72  GSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAADGQVRHAQILNS 131
           GSDD +V++W+W   +  L F+SGH +NVFQAK +P + D  +  CA DGQVR A++  +
Sbjct: 216 GSDDLKVVVWDWVRRKPVLEFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSAT 275

Query: 132 GRVE-TSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCYFKFHQHSNEL 191
              + T  +  H+G +HKLA+EP SP  F + GED +V        F    +  + ++ L
Sbjct: 276 HCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVV--------FTIDLRQDRPASRL 335

Query: 192 FTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPT 251
              +  +++ G      L  I ++P N   F V G D++ R+YD RK     +       
Sbjct: 336 VVTKEKESKVG------LYTIYVNPANTYQFAVGGRDQFVRIYDQRKINENVNNGV---L 395

Query: 252 DYYCPPHLIGDE-QVGITGLAYS-ELSELLVSYNDESIYLFN 291
             +CP HL+  E +  IT L YS + SELL SYNDE IYLFN
Sbjct: 396 KKFCPHHLVTSEAKANITCLVYSHDGSELLASYNDEDIYLFN 418

BLAST of MC04g0369 vs. NCBI nr
Match: XP_022136068.1 (DDB1- and CUL4-associated factor 8 [Momordica charantia])

HSP 1 Score: 558 bits (1439), Expect = 3.46e-197
Identity = 277/295 (93.90%), Postives = 279/295 (94.58%), Query Frame = 0

Query: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60
           MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60

Query: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120
           SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD
Sbjct: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120

Query: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCY 180
           GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQH          
Sbjct: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGA--- 180

Query: 181 FKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240
                 ++ELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG
Sbjct: 181 ------ASELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240

Query: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 295
           EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL
Sbjct: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 286

BLAST of MC04g0369 vs. NCBI nr
Match: TYK23393.1 (DDB1- and CUL4-associated factor 8 [Cucumis melo var. makuwa])

HSP 1 Score: 513 bits (1320), Expect = 4.29e-179
Identity = 255/295 (86.44%), Postives = 265/295 (89.83%), Query Frame = 0

Query: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60
           MNKRARIDDSNKA+VNVWKREVGELSTRNFAHRLGA +DLVLRLD+Y+KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120
           SFNAAGD+LVSGSDDRRVILWNWETG+VKLSFQSGHNNNVFQAKIMPYTDDR IVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCY 180
           GQVRHAQILNSG VETSLLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ           
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAV- 180

Query: 181 FKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240
                   ELFTCQS+DNRAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   
Sbjct: 181 --------ELFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGE 240

Query: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 295
           +GSTDFGQ  D YCPPHLIGDEQVGITGLA+SELSELLVSYNDESIYLFNRDMGL
Sbjct: 241 DGSTDFGQLVDSYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGL 286

BLAST of MC04g0369 vs. NCBI nr
Match: XP_008447917.1 (PREDICTED: DDB1- and CUL4-associated factor 8 [Cucumis melo])

HSP 1 Score: 513 bits (1320), Expect = 4.29e-179
Identity = 255/295 (86.44%), Postives = 265/295 (89.83%), Query Frame = 0

Query: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60
           MNKRARIDDSNKA+VNVWKREVGELSTRNFAHRLGA +DLVLRLD+Y+KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120
           SFNAAGD+LVSGSDDRRVILWNWETG+VKLSFQSGHNNNVFQAKIMPYTDDR IVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCY 180
           GQVRHAQILNSG VETSLLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ           
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAV- 180

Query: 181 FKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240
                   ELFTCQS+DNRAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   
Sbjct: 181 --------ELFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGE 240

Query: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 295
           +GSTDFGQ  D YCPPHLIGDEQVGITGLA+SELSELLVSYNDESIYLFNRDMGL
Sbjct: 241 DGSTDFGQLADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGL 286

BLAST of MC04g0369 vs. NCBI nr
Match: XP_038887987.1 (DDB1- and CUL4-associated factor 8-like [Benincasa hispida])

HSP 1 Score: 512 bits (1318), Expect = 8.94e-179
Identity = 254/295 (86.10%), Postives = 264/295 (89.49%), Query Frame = 0

Query: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60
           MNKRARIDDSNKA+VNVWKREVGELSTRNFAHRLGA +DLVLRLD+YRKLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYRKLERHKGCVNTV 60

Query: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120
           SFNAAGD+LVSGSDDRRVILWNWETG VKLSFQSGHNNNVFQAKIMPYTDDR IVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGHVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCY 180
           GQVRHAQILNSGRVET+LLGSH GRAHKLA EPGSPY+FYTCGEDGLVQ           
Sbjct: 121 GQVRHAQILNSGRVETALLGSHLGRAHKLAFEPGSPYMFYTCGEDGLVQRFDLRTGAAV- 180

Query: 181 FKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240
                   ELFTCQS+DNRAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   
Sbjct: 181 --------ELFTCQSVDNRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKSGE 240

Query: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 295
           +GSTDFGQ  D YCPPHLIGDEQVGITGLA+SE+SELLVSYNDESIYLFNRDMGL
Sbjct: 241 DGSTDFGQLVDCYCPPHLIGDEQVGITGLAFSEMSELLVSYNDESIYLFNRDMGL 286

BLAST of MC04g0369 vs. NCBI nr
Match: XP_004144864.1 (DDB1- and CUL4-associated factor 8 [Cucumis sativus] >KGN43277.1 hypothetical protein Csa_020173 [Cucumis sativus])

HSP 1 Score: 510 bits (1313), Expect = 4.97e-178
Identity = 253/295 (85.76%), Postives = 266/295 (90.17%), Query Frame = 0

Query: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60
           MNKRARIDDSNKA+VNVWKREVGELSTRNFAHRLGA +DLVLRLD+Y+KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120
           SFNAAGD+LVSGSDDRRVILWNWETG+VKLSFQSGHNNNVFQAKIMPYTDD+ IVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDQSIVTCAAD 120

Query: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCY 180
           GQVRHAQILNSG VETSLLG+H GRAHKLAIEPGSPY+FYTCGEDGLVQ           
Sbjct: 121 GQVRHAQILNSGHVETSLLGNHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTG---- 180

Query: 181 FKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240
                 + ELFTCQS+DNRAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   
Sbjct: 181 -----DAVELFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGE 240

Query: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 295
           +GSTDFGQ  D YCPPHLIGDEQVGITGLA+SELSELLVSYNDESIYLFNRDMGL
Sbjct: 241 DGSTDFGQLADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGL 286

BLAST of MC04g0369 vs. ExPASy TrEMBL
Match: A0A6J1C6L2 (DDB1- and CUL4-associated factor 8 OS=Momordica charantia OX=3673 GN=LOC111007852 PE=4 SV=1)

HSP 1 Score: 558 bits (1439), Expect = 1.68e-197
Identity = 277/295 (93.90%), Postives = 279/295 (94.58%), Query Frame = 0

Query: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60
           MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60

Query: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120
           SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD
Sbjct: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120

Query: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCY 180
           GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQH          
Sbjct: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGA--- 180

Query: 181 FKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240
                 ++ELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG
Sbjct: 181 ------ASELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240

Query: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 295
           EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL
Sbjct: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 286

BLAST of MC04g0369 vs. ExPASy TrEMBL
Match: A0A5D3DIH7 (DDB1-and CUL4-associated factor 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004590 PE=4 SV=1)

HSP 1 Score: 513 bits (1320), Expect = 2.08e-179
Identity = 255/295 (86.44%), Postives = 265/295 (89.83%), Query Frame = 0

Query: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60
           MNKRARIDDSNKA+VNVWKREVGELSTRNFAHRLGA +DLVLRLD+Y+KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120
           SFNAAGD+LVSGSDDRRVILWNWETG+VKLSFQSGHNNNVFQAKIMPYTDDR IVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCY 180
           GQVRHAQILNSG VETSLLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ           
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAV- 180

Query: 181 FKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240
                   ELFTCQS+DNRAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   
Sbjct: 181 --------ELFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGE 240

Query: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 295
           +GSTDFGQ  D YCPPHLIGDEQVGITGLA+SELSELLVSYNDESIYLFNRDMGL
Sbjct: 241 DGSTDFGQLVDSYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGL 286

BLAST of MC04g0369 vs. ExPASy TrEMBL
Match: A0A1S3BIJ4 (DDB1- and CUL4-associated factor 8 OS=Cucumis melo OX=3656 GN=LOC103490261 PE=4 SV=1)

HSP 1 Score: 513 bits (1320), Expect = 2.08e-179
Identity = 255/295 (86.44%), Postives = 265/295 (89.83%), Query Frame = 0

Query: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60
           MNKRARIDDSNKA+VNVWKREVGELSTRNFAHRLGA +DLVLRLD+Y+KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120
           SFNAAGD+LVSGSDDRRVILWNWETG+VKLSFQSGHNNNVFQAKIMPYTDDR IVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCY 180
           GQVRHAQILNSG VETSLLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ           
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAV- 180

Query: 181 FKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240
                   ELFTCQS+DNRAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   
Sbjct: 181 --------ELFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGE 240

Query: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 295
           +GSTDFGQ  D YCPPHLIGDEQVGITGLA+SELSELLVSYNDESIYLFNRDMGL
Sbjct: 241 DGSTDFGQLADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGL 286

BLAST of MC04g0369 vs. ExPASy TrEMBL
Match: A0A0A0K4R4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G016620 PE=4 SV=1)

HSP 1 Score: 510 bits (1313), Expect = 2.40e-178
Identity = 253/295 (85.76%), Postives = 266/295 (90.17%), Query Frame = 0

Query: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60
           MNKRARIDDSNKA+VNVWKREVGELSTRNFAHRLGA +DLVLRLD+Y+KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120
           SFNAAGD+LVSGSDDRRVILWNWETG+VKLSFQSGHNNNVFQAKIMPYTDD+ IVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDQSIVTCAAD 120

Query: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCY 180
           GQVRHAQILNSG VETSLLG+H GRAHKLAIEPGSPY+FYTCGEDGLVQ           
Sbjct: 121 GQVRHAQILNSGHVETSLLGNHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTG---- 180

Query: 181 FKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240
                 + ELFTCQS+DNRAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   
Sbjct: 181 -----DAVELFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGE 240

Query: 241 EGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 295
           +GSTDFGQ  D YCPPHLIGDEQVGITGLA+SELSELLVSYNDESIYLFNRDMGL
Sbjct: 241 DGSTDFGQLADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGL 286

BLAST of MC04g0369 vs. ExPASy TrEMBL
Match: A0A6J1HVC3 (DDB1- and CUL4-associated factor 8-like OS=Cucurbita maxima OX=3661 GN=LOC111468181 PE=4 SV=1)

HSP 1 Score: 480 bits (1235), Expect = 1.88e-166
Identity = 241/297 (81.14%), Postives = 256/297 (86.20%), Query Frame = 0

Query: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60
           MNKRAR DDS+KA+VN  KREVGE+STRNFA+RLGA +DLVLRL + RKLERHKGCVNTV
Sbjct: 1   MNKRARFDDSSKAVVNFLKREVGEISTRNFANRLGALEDLVLRLGLLRKLERHKGCVNTV 60

Query: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120
           SFNAAGDVLVSGSDDRRVILWNWE GQV++SFQSGHNNNVFQAK MPYTDDR IVTCAAD
Sbjct: 61  SFNAAGDVLVSGSDDRRVILWNWEAGQVEISFQSGHNNNVFQAKFMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCY 180
           GQVRHAQ+LN GRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQ           
Sbjct: 121 GQVRHAQVLNGGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQRFDLRTGAAV- 180

Query: 181 FKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKG 240
                   ELFTCQS+ NRAGYMS++QLNAI +DPRNPNL VVAGSDEYARLYDIRK  G
Sbjct: 181 --------ELFTCQSVGNRAGYMSTVQLNAIAMDPRNPNLVVVAGSDEYARLYDIRKCGG 240

Query: 241 EGST--DFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 295
           EGST  D G+P D YCPPHLI DEQVGITGLA+SELSELLVSYNDE IYLFNRDMGL
Sbjct: 241 EGSTSTDSGKPADCYCPPHLIADEQVGITGLAFSELSELLVSYNDEYIYLFNRDMGL 288

BLAST of MC04g0369 vs. TAIR 10
Match: AT4G35140.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 398.7 bits (1023), Expect = 4.2e-111
Identity = 193/300 (64.33%), Postives = 230/300 (76.67%), Query Frame = 0

Query: 1   MNKRARIDDSN-----KALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKG 60
           M+++AR   SN       +V+ W+REVG +S+RNF++R  AS++LVLRL+IY+KLE+HKG
Sbjct: 1   MSEKARRGRSNFNGLHHPVVDFWRREVGGISSRNFSNRFSASENLVLRLEIYKKLEKHKG 60

Query: 61  CVNTVSFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIV 120
           CVNTVSFNA GDVL+SGSDDRRV+LW+W+ G VKLSF SGH NNVFQAK MP++DDR IV
Sbjct: 61  CVNTVSFNAEGDVLISGSDDRRVVLWDWQLGNVKLSFHSGHANNVFQAKFMPFSDDRTIV 120

Query: 121 TCAADGQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYR 180
           TCAADG VR A IL   +VETS LG HQGRAHKL IEPG+P+IFYTCGEDGLVQ      
Sbjct: 121 TCAADGMVRRASILEGDKVETSFLGLHQGRAHKLCIEPGNPHIFYTCGEDGLVQRFDLRT 180

Query: 181 SFCCYFKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDI 240
                    +   ELFTC+S+D R   M +IQLNAI IDPRN NLF V G +EYARLYDI
Sbjct: 181 ---------EAPTELFTCRSVDPRRRNMDAIQLNAIAIDPRNSNLFAVGGMEEYARLYDI 240

Query: 241 RKYKGEGSTDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 296
           R+++GEG   F +  D++CPPHLIG+E VGITGLA+SE SELLVSYNDE IYLF   MGL
Sbjct: 241 RRFQGEGLNGFTRAADHFCPPHLIGNEDVGITGLAFSEQSELLVSYNDEFIYLFTPGMGL 291

BLAST of MC04g0369 vs. TAIR 10
Match: AT4G38480.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 362.8 bits (930), Expect = 2.5e-100
Identity = 176/293 (60.07%), Postives = 219/293 (74.74%), Query Frame = 0

Query: 3   KRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTVSF 62
           KR R D S   +VNVW+RE+G L  R+F++R  AS+DL+ RL + +KL++HKGCVNTVSF
Sbjct: 7   KRPRTDHS---VVNVWERELGLLPNRSFSNRFSASEDLLRRLGLDKKLDKHKGCVNTVSF 66

Query: 63  NAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAADGQ 122
           NA GD+L+SGSDDR+VILW+W+T  VKLSF SGH NN+FQAK MP++DDR IVT AAD Q
Sbjct: 67  NADGDILLSGSDDRQVILWDWQTASVKLSFDSGHFNNIFQAKFMPFSDDRTIVTSAADKQ 126

Query: 123 VRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCYFK 182
           VR+++IL SG+VETSLLG HQG  HKLA+EPGSP+ FYTCGEDG V+H            
Sbjct: 127 VRYSKILESGQVETSLLGKHQGPVHKLAVEPGSPFSFYTCGEDGAVKHFDLRT------- 186

Query: 183 FHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEG 242
             + +  LFTC+     A +   + L+AI +DPRNP L  VAG DEYAR+YDIR Y+ EG
Sbjct: 187 --RVATNLFTCK----EAKFNLVVYLHAIAVDPRNPGLLAVAGMDEYARVYDIRSYRSEG 246

Query: 243 STDFGQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGL 296
             +F QP D++CP HLIGD+ VGITGLA+S+ SELL SY+DE IYLF  DMGL
Sbjct: 247 WYNFTQPIDHFCPGHLIGDDHVGITGLAFSDQSELLASYSDEFIYLFTPDMGL 283

BLAST of MC04g0369 vs. TAIR 10
Match: AT3G45620.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 6.9e-74
Identity = 141/294 (47.96%), Postives = 180/294 (61.22%), Query Frame = 0

Query: 4   RARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTVSFN 63
           R R  +       ++ RE+G       + R+ AS+  V +LD+Y KL  H+GCVN V FN
Sbjct: 5   RRRNTEIGDGFTEIFNREIGFSHPITISRRISASEGRVKKLDLYGKLNGHEGCVNAVEFN 64

Query: 64  AAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAADGQV 123
           + GDVLVSGSDDR+++LWNW +G  KLS+ SGH  NVFQ K +P+TDDR I+T  ADGQV
Sbjct: 65  STGDVLVSGSDDRQIMLWNWLSGSRKLSYPSGHCENVFQTKFIPFTDDRTIITSGADGQV 124

Query: 124 RHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCYFKF 183
           R  QIL +G+VET  LG H GR +KLA+ PG P +FY+CGEDG VQH     +      +
Sbjct: 125 RLGQILENGKVETKRLGRHHGRVYKLAVLPGDPNVFYSCGEDGFVQHFDIRSNSATMVLY 184

Query: 184 HQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGS 243
                +   C+    R    S I+LN+I IDPRN     V GSDEYAR+YD R+ +    
Sbjct: 185 SSPFTQ--GCR----RHHSSSRIRLNSIAIDPRNSYYLAVGGSDEYARVYDTRRVQLAPV 244

Query: 244 TDF---GQPTDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMG 295
                   P + +CP HL     V ITGLAYS+  ELLVSYNDE IYLF ++MG
Sbjct: 245 CRHVLPDAPVNTFCPRHLRETNSVHITGLAYSKAGELLVSYNDELIYLFEKNMG 292

BLAST of MC04g0369 vs. TAIR 10
Match: AT3G45620.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 6.1e-70
Identity = 133/257 (51.75%), Postives = 165/257 (64.20%), Query Frame = 0

Query: 41  VLRLDIYRKLERHKGCVNTVSFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNV 100
           V +LD+Y KL  H+GCVN V FN+ GDVLVSGSDDR+++LWNW +G  KLS+ SGH  NV
Sbjct: 76  VKKLDLYGKLNGHEGCVNAVEFNSTGDVLVSGSDDRQIMLWNWLSGSRKLSYPSGHCENV 135

Query: 101 FQAKIMPYTDDRCIVTCAADGQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFY 160
           FQ K +P+TDDR I+T  ADGQVR  QIL +G+VET  LG H GR +KLA+ PG P +FY
Sbjct: 136 FQTKFIPFTDDRTIITSGADGQVRLGQILENGKVETKRLGRHHGRVYKLAVLPGDPNVFY 195

Query: 161 TCGEDGLVQHVSFYRSFCCYFKFHQHSNELFTCQSIDNRAGYMSSIQLNAITIDPRNPNL 220
           +CGEDG VQH     +      +     +   C+    R    S I+LN+I IDPRN   
Sbjct: 196 SCGEDGFVQHFDIRSNSATMVLYSSPFTQ--GCR----RHHSSSRIRLNSIAIDPRNSYY 255

Query: 221 FVVAGSDEYARLYDIRKYKGEGSTDF---GQPTDYYCPPHLIGDEQVGITGLAYSELSEL 280
             V GSDEYAR+YD R+ +            P + +CP HL     V ITGLAYS+  EL
Sbjct: 256 LAVGGSDEYARVYDTRRVQLAPVCRHVLPDAPVNTFCPRHLRETNSVHITGLAYSKAGEL 315

Query: 281 LVSYNDESIYLFNRDMG 295
           LVSYNDE IYLF ++MG
Sbjct: 316 LVSYNDELIYLFEKNMG 326

BLAST of MC04g0369 vs. TAIR 10
Match: AT5G10940.1 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 152.9 bits (385), Expect = 4.0e-37
Identity = 96/288 (33.33%), Postives = 149/288 (51.74%), Query Frame = 0

Query: 33  RLGASQDLVLRLDIYRKLERHKGCVNTVSFNAAGDVLVSGSDDRRVILWNWETGQVKLSF 92
           R+     LV RL   ++LE H+GCVN +++N+ G +L+SGSDD R+ +WN+ + ++  S 
Sbjct: 29  RMQFHSSLVRRLSQEQELEGHQGCVNALAWNSNGSLLISGSDDLRINIWNYSSRKLLHSI 88

Query: 93  QSGHNNNVFQAKIMPYTDDRCIVTCAADGQVRHAQILN-SGRVE-------TSLLGSHQG 152
            +GH  N+F  K +P T D  +V+ A D +VR       SGR E       ++L   H  
Sbjct: 89  DTGHTANIFCTKFVPETSDELVVSGAGDAEVRLFNTSRLSGRAEDDNAIIPSALYQCHTR 148

Query: 153 RAHKLAIEPGSPYIFYTCGEDGLVQHVSFYRSFCCYFKFHQHSNELFTCQSI--DNRAGY 212
           R  KLA+EPG+P + ++  EDG ++   F  S  C      H      C+S+  D R+G 
Sbjct: 149 RVKKLAVEPGNPNVVWSASEDGTLRQHDFRESTSCPPAGTAHQE----CRSVLLDLRSGA 208

Query: 213 --------MSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP----T 272
                     ++ L +  I    P+L +V GSD +ARLYD R      S+    P     
Sbjct: 209 KRALADPPKQTLSLKSCDISATRPHLLLVGGSDAFARLYDRRMLPPLASSRKRMPPPPCV 268

Query: 273 DYYCPPHLI--GDEQVGITGLAYS-ELSELLVSYNDESIYLFNRDMGL 296
           +Y+CP HL   G   + +T + +S    E+L+SY+ E +YL N + G+
Sbjct: 269 NYFCPMHLSERGRTNLHLTHVTFSPNGEEVLLSYSGEHVYLMNVNNGI 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8N7N55.1e-4537.94DDB1- and CUL4-associated factor 8 OS=Mus musculus OX=10090 GN=Dcaf8 PE=1 SV=1[more]
Q5TAQ91.5e-4437.59DDB1- and CUL4-associated factor 8 OS=Homo sapiens OX=9606 GN=DCAF8 PE=1 SV=1[more]
Q5U2M61.5e-4437.59DDB1- and CUL4-associated factor 8 OS=Rattus norvegicus OX=10116 GN=Dcaf8 PE=1 S... [more]
P0C7V85.6e-4437.82DDB1- and CUL4-associated factor 8-like protein 2 OS=Homo sapiens OX=9606 GN=DCA... [more]
Q6NRH15.6e-4436.88DDB1- and CUL4-associated factor 8 OS=Xenopus laevis OX=8355 GN=dcaf8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022136068.13.46e-19793.90DDB1- and CUL4-associated factor 8 [Momordica charantia][more]
TYK23393.14.29e-17986.44DDB1- and CUL4-associated factor 8 [Cucumis melo var. makuwa][more]
XP_008447917.14.29e-17986.44PREDICTED: DDB1- and CUL4-associated factor 8 [Cucumis melo][more]
XP_038887987.18.94e-17986.10DDB1- and CUL4-associated factor 8-like [Benincasa hispida][more]
XP_004144864.14.97e-17885.76DDB1- and CUL4-associated factor 8 [Cucumis sativus] >KGN43277.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
A0A6J1C6L21.68e-19793.90DDB1- and CUL4-associated factor 8 OS=Momordica charantia OX=3673 GN=LOC11100785... [more]
A0A5D3DIH72.08e-17986.44DDB1-and CUL4-associated factor 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BIJ42.08e-17986.44DDB1- and CUL4-associated factor 8 OS=Cucumis melo OX=3656 GN=LOC103490261 PE=4 ... [more]
A0A0A0K4R42.40e-17885.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G016620 PE=4 SV=1[more]
A0A6J1HVC31.88e-16681.14DDB1- and CUL4-associated factor 8-like OS=Cucurbita maxima OX=3661 GN=LOC111468... [more]
Match NameE-valueIdentityDescription
AT4G35140.14.2e-11164.33Transducin/WD40 repeat-like superfamily protein [more]
AT4G38480.12.5e-10060.07Transducin/WD40 repeat-like superfamily protein [more]
AT3G45620.16.9e-7447.96Transducin/WD40 repeat-like superfamily protein [more]
AT3G45620.26.1e-7051.75Transducin/WD40 repeat-like superfamily protein [more]
AT5G10940.14.0e-3733.33transducin family protein / WD-40 repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 131..172
e-value: 9.1
score: 11.0
coord: 85..127
e-value: 1.9
score: 15.2
coord: 251..290
e-value: 160.0
score: 3.2
coord: 43..82
e-value: 9.5E-6
score: 35.1
coord: 176..234
e-value: 11.0
score: 10.5
IPR001680WD40 repeatPFAMPF00400WD40coord: 49..82
e-value: 1.0E-4
score: 23.0
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 50..91
score: 14.017362
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 14..294
e-value: 7.8E-30
score: 105.9
IPR045151DDB1- and CUL4-associated factor 8-likePANTHERPTHR15574WD REPEAT DOMAIN-CONTAINING FAMILYcoord: 8..294
NoneNo IPR availablePANTHERPTHR15574:SF62WD40/YVTN REPEAT-LIKE-CONTAINING DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 8..294
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 50..87
score: 11.022865
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 42..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0369.1MC04g0369.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0080008 Cul4-RING E3 ubiquitin ligase complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding