MC04g0321 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0321 vs. ExPASy Swiss-Prot
Match: Q9HGL2 (Uncharacterized calcium-binding protein C800.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC800.10c PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 5.3e-15
Identity = 121/453 (26.71%), Postives = 180/453 (39.74%), Query Frame = 0

Query: 14  FDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQIGFLGRAEFYNALR 73
           FD  F+ AD    G I+G EAV F + SGLA QVL QIW +AD    GFL  + F  A+R
Sbjct: 13  FDQLFKIADKQDIGVITGEEAVPFLEKSGLAPQVLGQIWQIADAENRGFLTFSGFVIAMR 72

Query: 74  LVTVAQSKRELTPEIVKAALYSPAAAKIP-APQINVNIQPASQFNSTAALPPTPQSGIVA 133
           LV +AQ K     +          + KIP    I+++   +S+F             +  
Sbjct: 73  LVALAQEKLPFDYK---------KSGKIPYFADIHISGVDSSKF-------------VQL 132

Query: 134 PTPSQ-STGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQGFPGAGTASGPP 193
             P+  S+G GS       + LPP+ S E    +    T                     
Sbjct: 133 NRPNNVSSGDGSD-----GSFLPPISSDEMTRYQQMFTT-------------------VC 192

Query: 194 PTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTTSLPPRPQSAPG 253
           PT+  +  D AS   G              +P  T++       A +   +    + A  
Sbjct: 193 PTDGLMDGDRASSIFGR-------------APLSTEI------LARVWNLVDTHKRGALD 252

Query: 254 AKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDKLSASNKTSTSVTAPVSSVA 313
            +   T      +  +  NG+   P                             PVS   
Sbjct: 253 IREFNTG---MHIINLLLNGSLKSP-----------------------------PVSISP 312

Query: 314 QPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLSTGAHNSVSGQSQ 373
             IA A+S  S+      P L     R+ PV  PN      P S                
Sbjct: 313 SFIASAASTSSVSAPSQYPGLS----RSPPVQAPN-----IPVSD--------------- 342

Query: 374 RPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLKQVWDLSDQDND 433
            PW   +Q D+  + ++F  VDK   G ++G EA + FL+ +LP +VL Q+WDLSD +++
Sbjct: 373 -PWAIPSQ-DLTSFCQLFSNVDKAHKGYVSGSEAYSFFLASKLPEDVLAQIWDLSDTNSN 342

Query: 434 SMLSVREFCIALYLLERHREGHILPAMLPSNIM 465
             L++ EFCI+LYL++    G  LP +LPS+++
Sbjct: 433 GKLNIGEFCISLYLIKLKLSGKELPKVLPSSML 342

BLAST of MC04g0321 vs. ExPASy Swiss-Prot
Match: B3LF48 (EH domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=EHD2 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.4e-12
Identity = 35/81 (43.21%), Postives = 57/81 (70.37%), Query Frame = 0

Query: 13 LFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQIGFLGRAEFYNAL 72
          ++  +F  AD D DGR+SG +A  FF  S L++Q L Q+WA+AD ++ GFLG +EF  A+
Sbjct: 19 IYKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQVWAVADSKRQGFLGLSEFITAM 78

Query: 73 RLVTVAQSKRELTPEIVKAAL 94
          +LV++AQ   E+T +++K ++
Sbjct: 79 KLVSLAQEGHEITSDLLKGSI 99

BLAST of MC04g0321 vs. ExPASy Swiss-Prot
Match: O54916 (RalBP1-associated Eps domain-containing protein 1 OS=Mus musculus OX=10090 GN=Reps1 PE=1 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 7.4e-09
Identity = 109/440 (24.77%), Postives = 170/440 (38.64%), Query Frame = 0

Query: 29  ISGAEAVAFFQGSGLAKQVLAQIWALADPRQIGFLGRAEFYNALRLVTVAQSKRELTPEI 88
           +     +  F+ + L   V+ QI  L    ++G+ GR++FY AL+LV VAQS   L  E 
Sbjct: 30  VVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVES 89

Query: 89  VKAALYSPAAAKIPAPQINVNIQPASQFNSTAALPPTPQSGIVAPTPSQSTGLGSQVPRN 148
           +      P   +  A +     + A+ ++S +    +  SG++ P P +  G   + P +
Sbjct: 90  INTVKDLP-LPRFVASKNEQESRLAASYSSDSENQGS-YSGVIPPPPGR--GQVKKGPGS 149

Query: 149 VNANLPPVPSREGQSVRPPLATSNSAFRPAQGFPGAGTASGPPPTNSSISTDWASDGAGG 208
            +A + P PS E Q    P+ +      P Q           PPT+       +   +GG
Sbjct: 150 HDA-VQPRPSAEQQEPVSPVVS------PQQS----------PPTSPHTWRKHSRHPSGG 209

Query: 209 VQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTTSLPPRPQSAPGAKSSTTSPAESKVQGIS 268
               +S+ P  G  P  +    FG + AG          SA  A  S  SP   +   +S
Sbjct: 210 ----NSERPLTGPGPFWS---PFGDAQAG---------SSAGDAVWSGQSPPPPQDNWVS 269

Query: 269 GNGTASGPYFGGDIFSATPVTSDKLS---ASNKTSTSV-TAPVSSVAQPIARASSLDSLQ 328
                         F+ TP TS  L+   AS +  T+V T   ++ A  I R SS     
Sbjct: 270 --------------FADTPPTSALLTMHPASVQDQTTVRTVASAATANEIRRQSS----- 329

Query: 329 NSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLSTGAHNSVSGQSQRPWPRMTQTDVQK 388
                                                         + PW ++T    Q 
Sbjct: 330 --------------------------------------------SYEDPW-KITDEQRQY 368

Query: 389 YTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLKQVWDLSDQDNDSMLSVREFCIALY 448
           Y   F  +  D +G I G  A+  F   +LP   L  +W+LSD D D  L++ EFC A +
Sbjct: 390 YVNQFKTIQPDLNGFIPGSAAKEFFTKSKLPILELSHIWELSDFDKDGALTLDEFCAAFH 368

Query: 449 LLERHREGHILPAMLPSNIM 465
           L+   + G+ LP  LP ++M
Sbjct: 450 LVVARKNGYDLPEKLPESLM 368

BLAST of MC04g0321 vs. ExPASy Swiss-Prot
Match: Q6BNL1 (Actin cytoskeleton-regulatory complex protein PAN1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=PAN1 PE=3 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 1.2e-06
Identity = 115/468 (24.57%), Postives = 181/468 (38.68%), Query Frame = 0

Query: 14  FDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQIGFLGRAEFYNALR 73
           F+  FR A    +  ISG  A      SGL    LA+IW+LAD  + G L   EF  AL 
Sbjct: 132 FEHLFRTAVPKGEQAISGDSARDILLRSGLQPITLAEIWSLADTNKSGSLLFPEFALALH 191

Query: 74  LVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTAALPPTPQSGIVAP 133
           L  ++  K +  P ++     +   + + A   +V   PA+  ++T    P   SG  + 
Sbjct: 192 LCNLS-LKGDPLPTMLPEKWSNEVKSFVDAISFSVPENPANILSNT----PFASSG-ASS 251

Query: 134 TPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQGFPGAGTASGPPPT 193
            P  +  +  Q     N+   P  S + Q          + F  +Q      T + P P 
Sbjct: 252 NPINNDWMAPQATGFNNSGAVPSTSFQAQP---------TGFGASQEMMAQRTGNPPLPQ 311

Query: 194 ------NSSISTDWASDGAGGV------QGTSSQPPNRGVSPAGTQVGAFGQSSAGLTTS 253
                 +++++        GG       Q TS+  P +   P   Q   F   +   T  
Sbjct: 312 QATGFGSNNVAPLLPQRTGGGTLIPLQPQQTSNLIPAQKTGPLQPQTTGFQTQNPHQTGP 371

Query: 254 LPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDKLSASNKTST 313
              +PQS   A+     P +++  G     T   P   G      P ++     S     
Sbjct: 372 GALQPQSTGFAQRMNNGPLQAQTTGFQQQTTGFQPQSTG----FQPQSTGFQPQSTGFQP 431

Query: 314 SVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQ---PASSGL 373
             T P+   AQP  +       Q  F+  P G         G P    + Q   P+S   
Sbjct: 432 QQTGPLQ--AQPTGKPG-----QWGFVSTPTG---------GIPGMNAMEQHFLPSSQLP 491

Query: 374 STGAHNSVSG--QSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPR 433
           +    N++ G  ++   W  +T+ + Q Y  VF   D    G I GE A N+F    L R
Sbjct: 492 TNNLQNAMGGSLKTNVTW-SITKQEKQIYDGVFSAWDSRNKGFIDGEVAINIFGKSGLAR 551

Query: 434 EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIM 465
             L+ +W+L+D +N   L+  EF +A++L+ R   G  LP  LP  ++
Sbjct: 552 PDLESIWNLADTNNRGKLNKDEFAVAMHLVYRRLNGFDLPLRLPPELV 563

BLAST of MC04g0321 vs. ExPASy Swiss-Prot
Match: A7EKZ0 (Actin cytoskeleton-regulatory complex protein pan1 OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) OX=665079 GN=pan1 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.0e-06
Identity = 73/295 (24.75%), Postives = 120/295 (40.68%), Query Frame = 0

Query: 223 PAGTQVGAFG--QSSAGLTTSLPPRPQSA------------PGAKSSTTSPAESKVQGIS 282
           PAGT   +F   QS+A   T   P+P  +            PG +++ +   +S+  G  
Sbjct: 285 PAGTNAPSFDSRQSTATPPTIQQPQPMPSNSALLTAQMTGFPGQQNNFSGGFQSQPTGFQ 344

Query: 283 GNGTASGPYFGGDIFSATPVTSDKLSASNKTSTSVTAPVSSV-AQPIARASSLDSLQNSF 342
            +     P   G +      T    + SN  +T  T P   +   P   +S+L   Q   
Sbjct: 345 SSMQTGFPGQQGGL--QPQPTGFSQNMSNPQATGYTGPRPPMPPMPSNFSSNLSPAQTGM 404

Query: 343 MKPPLGNQSLRNQPVGKPNQQGIVQPASSGL------------------STGAHNSVSGQ 402
               +    L +QP G P Q G+V   ++GL                         ++G 
Sbjct: 405 QGGMIA--PLNSQPTGVPGQWGLVNAPATGLPNIDLLQSRMMPQQGREQGNFTTAGITGN 464

Query: 403 SQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLKQVWDLSDQD 462
           +  PW  +T+ +  +Y  VF   D    G I G+ A  +F    L +  L+++W LSD  
Sbjct: 465 AVIPW-AVTKEEKTRYDSVFKAWDGFGKGFIGGDVAIEVFGQSGLEKPDLERIWTLSDHG 524

Query: 463 NDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAASNFSNA 485
           N   L++ EF +A++L+ R   G+ LPA LP  +          V P+  NF+++
Sbjct: 525 NKGKLNMDEFAVAMHLIYRKLNGYPLPAQLPPEL----------VPPSTRNFNDS 564

BLAST of MC04g0321 vs. NCBI nr
Match: XP_022135768.1 (epidermal growth factor receptor substrate 15-like 1 [Momordica charantia])

HSP 1 Score: 2086 bits (5405), Expect = 0.0
Identity = 1085/1118 (97.05%), Postives = 1085/1118 (97.05%), Query Frame = 0

Query: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60
            MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI
Sbjct: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60

Query: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120
            GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120

Query: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180
            ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG
Sbjct: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180

Query: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240
            FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT
Sbjct: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240

Query: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDKLSASNKTS 300
            SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDKLSASNKTS
Sbjct: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDKLSASNKTS 300

Query: 301  TSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLST 360
            TSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLST
Sbjct: 301  TSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLST 360

Query: 361  GAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLK 420
            GAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLK
Sbjct: 361  GAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLK 420

Query: 421  QVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAASN 480
            QVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAASN
Sbjct: 421  QVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAASN 480

Query: 481  FSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVLE 540
            FSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVLE
Sbjct: 481  FSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVLE 540

Query: 541  KNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEYFRTKMQ 600
            KNLVSQLSTEEQKSLNSKFQEAADAEKK         VEELEKEILDSRQKIEYFRTKMQ
Sbjct: 541  KNLVSQLSTEEQKSLNSKFQEAADAEKK---------VEELEKEILDSRQKIEYFRTKMQ 600

Query: 601  ELV------------------------DSLAKKYEEKYKQSGDVASRLTVEESTFRDIQE 660
            ELV                        DSLAKKYEEKYKQSGDVASRLTVEESTFRDIQE
Sbjct: 601  ELVLYKSRCDNRLNEISERVSSDKREVDSLAKKYEEKYKQSGDVASRLTVEESTFRDIQE 660

Query: 661  KKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTELP 720
            KKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTELP
Sbjct: 661  KKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTELP 720

Query: 721  FGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVDS 780
            FGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVDS
Sbjct: 721  FGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVDS 780

Query: 781  QKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAPNS 840
            QKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAPNS
Sbjct: 781  QKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAPNS 840

Query: 841  PFASSAIGSPKESMDSNFGKAAGFDASPRDKDAHSDHGGVGSVFSGDKSYDEPAWGRFDT 900
            PFASSAIGSPKESMDSNFGKAAGFDASPRDKDAHSDHGGVGSVFSGDKSYDEPAWGRFDT
Sbjct: 841  PFASSAIGSPKESMDSNFGKAAGFDASPRDKDAHSDHGGVGSVFSGDKSYDEPAWGRFDT 900

Query: 901  NDDVDSVWGFNAGGSTKTDNDLNRDSYFFDSGELGLNPIRTDPFPAKRSTFAFDESVPST 960
            NDDVDSVWGFNAGGSTKTDNDLNRDSYFFDSGELGLNPIRTDPFPAKRSTFAFDESVPST
Sbjct: 901  NDDVDSVWGFNAGGSTKTDNDLNRDSYFFDSGELGLNPIRTDPFPAKRSTFAFDESVPST 960

Query: 961  PLFNSGNSPHNFREGSEPSFDSFLRFDSFGMHDSGIFPPQDNTFSRFDSMRSSRDFDQGH 1020
            PLFNSGNSPHNFREGSEPSFDSFLRFDSFGMHDSGIFPPQDNTFSRFDSMRSSRDFDQGH
Sbjct: 961  PLFNSGNSPHNFREGSEPSFDSFLRFDSFGMHDSGIFPPQDNTFSRFDSMRSSRDFDQGH 1020

Query: 1021 GFSSFGPPDTPHSSRDFNRGHGPSSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQG 1080
            GFSSFGPPDTPHSSRDFNRGHGPSSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQG
Sbjct: 1021 GFSSFGPPDTPHSSRDFNRGHGPSSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQG 1080

Query: 1081 HGFPSFDDAADPFGATGPFRASLDNQTPKRGSDNWSAF 1094
            HGFPSFDDAADPFGATGPFRASLDNQTPKRGSDNWSAF
Sbjct: 1081 HGFPSFDDAADPFGATGPFRASLDNQTPKRGSDNWSAF 1109

BLAST of MC04g0321 vs. NCBI nr
Match: XP_038887522.1 (epidermal growth factor receptor substrate 15-like 1 [Benincasa hispida] >XP_038887523.1 epidermal growth factor receptor substrate 15-like 1 [Benincasa hispida])

HSP 1 Score: 1697 bits (4395), Expect = 0.0
Identity = 922/1158 (79.62%), Postives = 971/1158 (83.85%), Query Frame = 0

Query: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60
            MAS QN APNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGL KQVLAQIWAL+DPRQI
Sbjct: 1    MASVQNPAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60

Query: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120
            GFLGRAEFYNALRLVTVAQSKRELTP+IVKAAL+SPAAAKIPAPQIN N QPASQFNSTA
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPDIVKAALFSPAAAKIPAPQINFNTQPASQFNSTA 120

Query: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180
            A P TPQSGIVA TPSQS+GL SQVPRNV  N+PPV SRE QSVRPP ATSNSA R AQG
Sbjct: 121  AAP-TPQSGIVAQTPSQSSGLDSQVPRNVTTNVPPVSSRESQSVRPPSATSNSALRSAQG 180

Query: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240
            FPG GT SGPPPTNSSIS DW S+   GVQGT SQPPNRGVSPAGTQVG FGQSSAGLT 
Sbjct: 181  FPGVGTVSGPPPTNSSISNDWVSERTSGVQGTPSQPPNRGVSPAGTQVG-FGQSSAGLTA 240

Query: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDK-LSASNKT 300
            SLPPRPQSAPG   +  SP ESKVQGISGNGTA G YFG D F+ATP+ S + ++A NKT
Sbjct: 241  SLPPRPQSAPGVTLAAPSPVESKVQGISGNGTAPGSYFGRDAFAATPIPSKQDVTADNKT 300

Query: 301  STSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLS 360
            S     PVS+V QPI RASSLDSLQ+SFMKPPL NQ+ RNQ +GKPNQQGI+Q ASSGL 
Sbjct: 301  SPLAAVPVSTVTQPIVRASSLDSLQSSFMKPPLANQAQRNQALGKPNQQGILQSASSGLP 360

Query: 361  TGAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVL 420
            TG+ NSVSGQSQRPWPRMTQ DVQKYTKVFVEVDKDRDGKITG EARNLFLSWRLPREVL
Sbjct: 361  TGSQNSVSGQSQRPWPRMTQNDVQKYTKVFVEVDKDRDGKITGLEARNLFLSWRLPREVL 420

Query: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAAS 480
            KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAAS
Sbjct: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAAS 480

Query: 481  NFSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVL 540
            N+SNAAWRPTAGYQQHQG+PGSGN  GA T GV+PP PA  S  E EP T++PKSKVPVL
Sbjct: 481  NYSNAAWRPTAGYQQHQGVPGSGNLQGARTVGVRPPIPATASSVEGEPQTSQPKSKVPVL 540

Query: 541  EKNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEYFRTKM 600
            EKNLVSQLSTEEQ SLNSKFQEAADAEKK         VEELEKEIL+SRQKIEY+RTKM
Sbjct: 541  EKNLVSQLSTEEQNSLNSKFQEAADAEKK---------VEELEKEILESRQKIEYYRTKM 600

Query: 601  QELV------------------------DSLAKKYEEKYKQSGDVASRLTVEESTFRDIQ 660
            QELV                        +SLAKKYEEKYKQSGDVASRLTVEE+TFRDIQ
Sbjct: 601  QELVLYKSRCDNRLNEISERVSSDKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQ 660

Query: 661  EKKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTEL 720
            EKKME+YQAIVKM QDGGADGVLQARADRIQSDIEELVK LNERCK+YGL AKPITL EL
Sbjct: 661  EKKMELYQAIVKMEQDGGADGVLQARADRIQSDIEELVKSLNERCKTYGLWAKPITLNEL 720

Query: 721  PFGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVD 780
            PFGWQPGIQVGAADWDEDWDKFEDEGF+VVKELTLDVQNVIAPPKQKSKS QK K   +D
Sbjct: 721  PFGWQPGIQVGAADWDEDWDKFEDEGFSVVKELTLDVQNVIAPPKQKSKSFQKGK---LD 780

Query: 781  SQKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAPN 840
            SQ V PA D DTKE    G S P+ DTK EK  SMDETAVENGS HDNKSE  SAKSAPN
Sbjct: 781  SQNVTPAADDDTKE----GDSAPNADTKREKHASMDETAVENGSAHDNKSEDGSAKSAPN 840

Query: 841  SPF--------------------ASSAIGSPKESMDSNFGKAAGFDASPRDKDAHSDHGG 900
            SPF                    ASS IGSPKE MDSNFGKAAGFDASPRDKD  SDHGG
Sbjct: 841  SPFTAKSAPNSPFAPKSAPSSPFASSVIGSPKEYMDSNFGKAAGFDASPRDKDTLSDHGG 900

Query: 901  VGSVFSGDKSYDEPAWGRFDTNDDVDSVWGFNAGGSTKTDNDLNRDSYFFDSGELGLNPI 960
             GSVFSGDKSYDEPAWG FDTNDD+DSVWGFNAGGSTKTD+D+NRD+YFFDSG+LGLNPI
Sbjct: 901  AGSVFSGDKSYDEPAWGTFDTNDDMDSVWGFNAGGSTKTDSDVNRDNYFFDSGDLGLNPI 960

Query: 961  RTDPFPAKRSTFAFDESVPSTPLFNSGNSPHNFREGSEPSFDSFLRFDSFGMHDSGIFPP 1020
            RTDPF AKRSTFAFDESVPSTP+FNSGNSPHN+ EGSEPSFDSF RFD+  +HDSG FPP
Sbjct: 961  RTDPFQAKRSTFAFDESVPSTPIFNSGNSPHNYHEGSEPSFDSFSRFDTSSVHDSGFFPP 1020

Query: 1021 QDNTFSRFDSMRSSRDFDQGHGFSSFGPPDTPHSSRDFNRGHGPSSLSRFDSMRSSKDFD 1080
            +D  FSRFDSMRSSRDFDQG GFSSFG  DT HSSRDF++  GPSSL++FDSMRSSKDFD
Sbjct: 1021 RD-AFSRFDSMRSSRDFDQGPGFSSFGQFDTTHSSRDFDQS-GPSSLTQFDSMRSSKDFD 1080

Query: 1081 QGFPSLARFDS-------------------MRSSKDFDQGHGFPSFDDAADPFGATGPFR 1094
            QGFPSL+RFDS                   MRSSKDFDQGHGFPSFDD  DPFG+TGPFR
Sbjct: 1081 QGFPSLSRFDSTRSSRDFDQGFPSFSRFDSMRSSKDFDQGHGFPSFDDP-DPFGSTGPFR 1137

BLAST of MC04g0321 vs. NCBI nr
Match: KAG6572175.1 (hypothetical protein SDJN03_28903, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1681 bits (4354), Expect = 0.0
Identity = 912/1167 (78.15%), Postives = 965/1167 (82.69%), Query Frame = 0

Query: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60
            MASAQN APNVDLFDAYFRRADLDRDGRISGAEAV+FFQGSGL K VL QIWAL+DPRQI
Sbjct: 1    MASAQNPAPNVDLFDAYFRRADLDRDGRISGAEAVSFFQGSGLPKPVLFQIWALSDPRQI 60

Query: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120
            GFLGRAEFYNALRLVTVAQSKRELTP+IVKAAL+SPAA+KIPAPQIN N QPA QFNSTA
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPDIVKAALFSPAASKIPAPQINFNTQPAPQFNSTA 120

Query: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180
             +  TP SGI  PTPS+S+GL  QVP+NV  N+P V SRE Q VRPPLATSNSAFRPAQG
Sbjct: 121  PVS-TPLSGIGTPTPSRSSGLELQVPQNVTTNVPLVSSRESQLVRPPLATSNSAFRPAQG 180

Query: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240
            F G GT SGPPPTNS IS DW S+ A G+QGT SQPPNRGVSPAG QVG FGQSSAGLTT
Sbjct: 181  FSGVGTVSGPPPTNSIISNDWVSERASGIQGTPSQPPNRGVSPAGMQVG-FGQSSAGLTT 240

Query: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDK-LSASNKT 300
            S P RPQSAPG   + +SP ESKVQGISGNGTASG YFG D F ATPV+S + + A NKT
Sbjct: 241  SQPQRPQSAPGVMPAASSPVESKVQGISGNGTASGSYFGRDAFPATPVSSRQDVPAGNKT 300

Query: 301  STSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLS 360
            STSVT PVSSV QPI RA+SLDSLQNSFMKPPL NQ+LRNQP  KPNQQ I+QPASSGLS
Sbjct: 301  STSVTVPVSSVTQPIVRATSLDSLQNSFMKPPLANQALRNQPHVKPNQQSILQPASSGLS 360

Query: 361  TGAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVL 420
            TG  NSV GQSQRPWPRMTQ DVQKYTKVFVEVDKDRDGKIT +EARNLFLSWRLPREVL
Sbjct: 361  TGLQNSVYGQSQRPWPRMTQNDVQKYTKVFVEVDKDRDGKITAQEARNLFLSWRLPREVL 420

Query: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAAS 480
             QVWDLSDQDNDSMLSVREFCIALYLLERHREGH+LPAMLPSNIMFDF SNGHPVTPAAS
Sbjct: 421  MQVWDLSDQDNDSMLSVREFCIALYLLERHREGHVLPAMLPSNIMFDFPSNGHPVTPAAS 480

Query: 481  NFSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVL 540
            N SNAAWR TAG+QQHQG+PGSGN HGAPT GV+PP PAA    EDEP TN+PKSKVPVL
Sbjct: 481  NHSNAAWRTTAGHQQHQGVPGSGNLHGAPTIGVRPPIPAAALPVEDEPQTNQPKSKVPVL 540

Query: 541  EKNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEYFRTKM 600
            +KNLVSQLSTEEQ SLNSKFQEA DAEKK         VEELEKEILDSRQKIEY+RTKM
Sbjct: 541  DKNLVSQLSTEEQNSLNSKFQEAEDAEKK---------VEELEKEILDSRQKIEYYRTKM 600

Query: 601  QEL------------------------VDSLAKKYEEKYKQSGDVASRLTVEESTFRDIQ 660
            QEL                        V++LAKKYEEKYKQSGDVASRLTVEE+TFRDIQ
Sbjct: 601  QELILYKSRCDNRLNEISERVSSEKREVETLAKKYEEKYKQSGDVASRLTVEEATFRDIQ 660

Query: 661  EKKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTEL 720
            EKKME+Y  IVKM QD   DGVLQARADRIQSDIEELVK LNERCKSYGLRAKPITLTEL
Sbjct: 661  EKKMELYHTIVKMEQDISEDGVLQARADRIQSDIEELVKVLNERCKSYGLRAKPITLTEL 720

Query: 721  PFGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVD 780
            PFGWQPGIQVGAADWDEDWDKFE+EGF+VVKELTLDVQNVIAPPKQKSKSVQKEKG+ VD
Sbjct: 721  PFGWQPGIQVGAADWDEDWDKFEEEGFSVVKELTLDVQNVIAPPKQKSKSVQKEKGSVVD 780

Query: 781  SQKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAPN 840
            SQ V PA DADTKE    GGS PD DTKGEKPPS+DETAVENGS HDNKSE  SAKSAPN
Sbjct: 781  SQNVTPAADADTKE----GGSAPDADTKGEKPPSIDETAVENGSAHDNKSENGSAKSAPN 840

Query: 841  SPFA------------------------------SSAIGSPKESMDSNFGKAAGFDASPR 900
            SPFA                              SS IGSPKE MDS+FGKAAGFDASPR
Sbjct: 841  SPFAPKSAPSSPFATKSAPSSPFAPKSAPSSPFASSVIGSPKEYMDSSFGKAAGFDASPR 900

Query: 901  DKDAHSDHGGVGSVFSGDKSYDEPAWGRFDTNDDVDSVWGFNAGGSTKTDNDLNRDSYFF 960
             KDA SDHGG GSVFSGDKSYDEPAWG FD NDD+DSVWGFNAGGSTK DND+ RD+Y+F
Sbjct: 901  AKDALSDHGGAGSVFSGDKSYDEPAWGAFDANDDIDSVWGFNAGGSTKADNDVTRDNYYF 960

Query: 961  DSGELGLNPIRTDPFPAKRSTFAFDESVPSTPLFNSGNSPHNFREGSEPSFDSFLRFDSF 1020
            DSG+ GLNPIRTDPF AKRSTFAFDESVPSTPL NSGNSP+N+ +GSEPSFDSF RFDS 
Sbjct: 961  DSGDFGLNPIRTDPFQAKRSTFAFDESVPSTPLVNSGNSPNNYHDGSEPSFDSFSRFDSS 1020

Query: 1021 GMHDSGIFPPQDNTFSRFDSMRSSRDFDQGHGFSSFGPPDTPHSSRDFNRG--------- 1080
             +HDSG FPP+D TF+RFDSMRSSRDFDQG GFSSF   DT +S+RDFN+G         
Sbjct: 1021 SVHDSGFFPPKD-TFTRFDSMRSSRDFDQGPGFSSFSQFDTTYSNRDFNQGGSSSLTRFD 1080

Query: 1081 ---------HGPSSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQGHGFPSFDDAAD 1094
                      G  SLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQGHGFPSFDD  D
Sbjct: 1081 SMRSSKDFDQGSPSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQGHGFPSFDDP-D 1140

BLAST of MC04g0321 vs. NCBI nr
Match: XP_022952345.1 (epidermal growth factor receptor substrate 15-like 1 [Cucurbita moschata])

HSP 1 Score: 1678 bits (4346), Expect = 0.0
Identity = 910/1167 (77.98%), Postives = 964/1167 (82.60%), Query Frame = 0

Query: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60
            MASAQN APNVDLFDAYFRRADLDRDGRISGAEAV+FFQGSGL K VL QIWAL+DPRQI
Sbjct: 1    MASAQNPAPNVDLFDAYFRRADLDRDGRISGAEAVSFFQGSGLPKPVLFQIWALSDPRQI 60

Query: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120
            GFLGRAEFYNALRLVTVAQSKRELTP+IVKAAL+SPAA+KIPAPQIN N QPA QFNSTA
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPDIVKAALFSPAASKIPAPQINFNTQPAPQFNSTA 120

Query: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180
             +  TP SGI  PTPS+S+GL  QVPRNV  N+P V SRE Q VRPPLATSNSAFRPAQG
Sbjct: 121  PVS-TPLSGIGTPTPSRSSGLELQVPRNVTTNVPLVSSRESQLVRPPLATSNSAFRPAQG 180

Query: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240
            F G GT SGPPPTNS IS DW S+ A G+QGT SQPPNRGVSPAG QVG FGQSSAGLTT
Sbjct: 181  FSGVGTVSGPPPTNSIISNDWVSERASGIQGTPSQPPNRGVSPAGMQVG-FGQSSAGLTT 240

Query: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDK-LSASNKT 300
            S P RPQSAPG   + +SP ESKVQGISGNGTASG YFG D F ATPV+S + + A NKT
Sbjct: 241  SQPQRPQSAPGVMPAASSPVESKVQGISGNGTASGSYFGRDAFPATPVSSKQDVPAGNKT 300

Query: 301  STSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLS 360
            STSVT PVSSV QPI RA+SLDSLQNSFMKPPL NQ+LRNQP  KPNQQ I+QPASSGLS
Sbjct: 301  STSVTVPVSSVTQPIVRATSLDSLQNSFMKPPLANQALRNQPHVKPNQQSILQPASSGLS 360

Query: 361  TGAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVL 420
            TG  NSV GQSQRPWPRMTQ DVQKYTKVFVEVDKDRDGKIT +EARNLFLSWRLPREVL
Sbjct: 361  TGLQNSVYGQSQRPWPRMTQNDVQKYTKVFVEVDKDRDGKITAQEARNLFLSWRLPREVL 420

Query: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAAS 480
             QVWDLSDQDNDSMLSVREFCIALYLLERHREGH+LPAMLPSNIMFDF SNGHPVTPAAS
Sbjct: 421  MQVWDLSDQDNDSMLSVREFCIALYLLERHREGHVLPAMLPSNIMFDFPSNGHPVTPAAS 480

Query: 481  NFSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVL 540
            N SNAAWR TAG+QQHQG+PGSGN HGAPT GV+PP PAA    EDEP TN+PKSKVP+L
Sbjct: 481  NHSNAAWRTTAGHQQHQGVPGSGNLHGAPTIGVRPPIPAAALPVEDEPQTNQPKSKVPLL 540

Query: 541  EKNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEYFRTKM 600
            +KNLVSQLSTEEQ SLNSKFQEA DAEKK         VEELEKEILDSRQKIEY+RTKM
Sbjct: 541  DKNLVSQLSTEEQNSLNSKFQEAEDAEKK---------VEELEKEILDSRQKIEYYRTKM 600

Query: 601  QEL------------------------VDSLAKKYEEKYKQSGDVASRLTVEESTFRDIQ 660
            QEL                        V+SLAKKYEEKYKQSGDVASRLTVEE+TFRDIQ
Sbjct: 601  QELILYKSRCDNRLNEISERVSSEKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQ 660

Query: 661  EKKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTEL 720
            EKKME+Y  IVKM QD   DGVLQARADRIQSDIEELVK LNERCKSYGLRAKPITLTEL
Sbjct: 661  EKKMELYHTIVKMEQDISEDGVLQARADRIQSDIEELVKVLNERCKSYGLRAKPITLTEL 720

Query: 721  PFGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVD 780
            PFGWQPGIQVGAADWDEDWDKFE+EGF+VVKELTLDVQNVIAPPKQKSKSVQKEKG+ VD
Sbjct: 721  PFGWQPGIQVGAADWDEDWDKFEEEGFSVVKELTLDVQNVIAPPKQKSKSVQKEKGSTVD 780

Query: 781  SQKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAPN 840
            SQ V PA DAD KE    GGS PD DTKGEKPPS+DETAVENGS HDNKSE  SAKSAPN
Sbjct: 781  SQNVTPAADADAKE----GGSAPDADTKGEKPPSIDETAVENGSAHDNKSENGSAKSAPN 840

Query: 841  SPFA------------------------------SSAIGSPKESMDSNFGKAAGFDASPR 900
            SPFA                              SS IGSPKE MDS+FGKAAGFDASPR
Sbjct: 841  SPFAPKSAPSSPFATKSAPSSPFAPKSAPSSPFASSVIGSPKEYMDSSFGKAAGFDASPR 900

Query: 901  DKDAHSDHGGVGSVFSGDKSYDEPAWGRFDTNDDVDSVWGFNAGGSTKTDNDLNRDSYFF 960
             KDA SDHGG GSVFSGDKSYDEPAWG FD NDD+DSVWGFNAGGSTK DND+ RD+Y+F
Sbjct: 901  AKDALSDHGGAGSVFSGDKSYDEPAWGAFDANDDIDSVWGFNAGGSTKADNDVTRDNYYF 960

Query: 961  DSGELGLNPIRTDPFPAKRSTFAFDESVPSTPLFNSGNSPHNFREGSEPSFDSFLRFDSF 1020
            DSG+ GLNPIRTDPF AKRSTFAFDESVPSTPL NSGNSP+N+ +GSEPSFDSF RFDS 
Sbjct: 961  DSGDFGLNPIRTDPFQAKRSTFAFDESVPSTPLVNSGNSPNNYHDGSEPSFDSFSRFDSS 1020

Query: 1021 GMHDSGIFPPQDNTFSRFDSMRSSRDFDQGHGFSSFGPPDTPHSSRDFNRG--------- 1080
             +HDSG FPP+D TF+RFDSMRSSRDFDQG GFSSF   DT +++RDF++G         
Sbjct: 1021 SVHDSGFFPPKD-TFTRFDSMRSSRDFDQGPGFSSFSQFDTTYNNRDFDQGGSSSLTRFD 1080

Query: 1081 ---------HGPSSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQGHGFPSFDDAAD 1094
                      G  SLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQGHGFPSFDD  D
Sbjct: 1081 SMRSSKDFDQGSPSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQGHGFPSFDDP-D 1140

BLAST of MC04g0321 vs. NCBI nr
Match: KAG7022768.1 (hypothetical protein SDJN02_16504 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1678 bits (4345), Expect = 0.0
Identity = 912/1165 (78.28%), Postives = 963/1165 (82.66%), Query Frame = 0

Query: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60
            MASAQN APNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGL KQVLAQIWAL+DPRQI
Sbjct: 1    MASAQNSAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60

Query: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120
            GFLGRAEF NALRLVTVAQSKRELTPEIVKAAL+SPAA+KIPAPQIN N  PASQFNST 
Sbjct: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120

Query: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180
            ALP TPQ+GI  PTPSQ +GL SQV R+V     PVPSRE QSVRPPLATSNSAF P QG
Sbjct: 121  ALP-TPQTGIGTPTPSQGSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQG 180

Query: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240
            F G G  SGPPPTNSSIS DW SD AGGVQGT SQPPNRG SPAGTQVG FGQSSAGL T
Sbjct: 181  FSGVGPVSGPPPTNSSISNDWVSDHAGGVQGTPSQPPNRGFSPAGTQVG-FGQSSAGLIT 240

Query: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTS------DKLS 300
            SLPPRPQ+APG KS+T SP +S VQG SGNG ASG YFGG  F+A+ V S      DKLS
Sbjct: 241  SLPPRPQTAPGIKSATPSPVDSNVQGFSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLS 300

Query: 301  ASNKTSTSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPA 360
              N +STS T PV S  QPI RA SLDSLQNSFMKPPL NQ  RNQP+GKPNQQ I  PA
Sbjct: 301  TGNISSTSATVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPA 360

Query: 361  SSGLSTGAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRL 420
            SSGLSTG+ NSVSGQSQRPWPRMTQTDVQKYTKVFV VDKDRDGKITGEEARNLFLSWRL
Sbjct: 361  SSGLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRL 420

Query: 421  PREVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPV 480
            PREVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSN++FDFSSNGHPV
Sbjct: 421  PREVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPV 480

Query: 481  TPAASNFSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKS 540
            TPAASN+SNAAWRPTAGYQQHQG+PGSGN H AP  GVQPP PAA S  EDEP +NKPKS
Sbjct: 481  TPAASNYSNAAWRPTAGYQQHQGVPGSGNVHVAPVVGVQPPTPAAASPVEDEPQSNKPKS 540

Query: 541  KVPVLEKNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEY 600
            KVPVLEKNLVSQLSTEEQ SLNSKFQEAADAEKK         VEELEKEI +SRQKIEY
Sbjct: 541  KVPVLEKNLVSQLSTEEQNSLNSKFQEAADAEKK---------VEELEKEISESRQKIEY 600

Query: 601  FRTKMQELV------------------------DSLAKKYEEKYKQSGDVASRLTVEEST 660
            +RTKMQELV                        +SLAKKYEEKYKQSGDVASRLTVEE+T
Sbjct: 601  YRTKMQELVLYKSRCDNRLNEISERVSSEKREVESLAKKYEEKYKQSGDVASRLTVEEAT 660

Query: 661  FRDIQEKKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPI 720
            FRDIQEKKME+Y AIVKM QD   DG++Q RADRIQ+DIEELVK LNERCKSYGLRAKPI
Sbjct: 661  FRDIQEKKMELYHAIVKMEQDVSEDGIIQVRADRIQADIEELVKSLNERCKSYGLRAKPI 720

Query: 721  TLTELPFGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKS--VQK 780
            TL+ELPFGWQPGIQVGAADWDEDWDKFE+EGFTVVKELTLDVQNVIAPPKQKSKS  VQK
Sbjct: 721  TLSELPFGWQPGIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQK 780

Query: 781  EKGAAVDSQKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGE 840
            E+G+A+DSQKV PAPD DTK+    GGS PD D+KGEKPPSMDE AVENGS HDNKSE  
Sbjct: 781  EQGSAIDSQKVTPAPDVDTKD----GGSTPDADSKGEKPPSMDEKAVENGSVHDNKSEDG 840

Query: 841  SAKSAPNSPFASSAIGSPKESMDSNFGKAAGFDASPRDKD-------------------- 900
            SA+SAPNSPFASS I SPKE  DSNFGKAAGFDASPRDKD                    
Sbjct: 841  SARSAPNSPFASSVIESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAGFDASPRD 900

Query: 901  --AHSDHGGVGSVFSGDKSYDEPAWGRFDTNDDVDSVWGFNAGGSTKTDNDLNRDSYFFD 960
              A SDHGG GSVFSGDKSYDEPAWG FD NDDVDSVWGFNAGGSTKTD D+NRD+YFFD
Sbjct: 901  KDALSDHGGAGSVFSGDKSYDEPAWGTFDMNDDVDSVWGFNAGGSTKTDTDVNRDNYFFD 960

Query: 961  SGELGLNPIRTDPFPAKRSTFAFDESVPSTPLFNSGNSPHNFREGSEPSFDSFLRFDSFG 1020
            SG+LGLNPIRT+PF AKRSTFAFDESVPSTPLFNSGNSP+N+ EGSEPSF+SF RFDS  
Sbjct: 961  SGDLGLNPIRTEPFQAKRSTFAFDESVPSTPLFNSGNSPNNYHEGSEPSFESFSRFDSSS 1020

Query: 1021 MHDSGIFPPQDNTFSRFDSMRSSRDFDQGHGFSSFGPPDTPHSSRDFNRG---------- 1080
            +H+ G FPPQD TF+RFDSMRSS+DFDQG GFS FG  DT  SSRDF++G          
Sbjct: 1021 VHEGG-FPPQD-TFARFDSMRSSKDFDQGPGFSPFGQFDTSRSSRDFDQGGSSLTRFDSM 1080

Query: 1081 -------HGPSSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQGHGFPSFDDAADPF 1094
                    G  S SRFDSMRSSKDFDQ FPSL+RFDSMRSSKDFDQGHGFPSFDDA   F
Sbjct: 1081 RSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLSRFDSMRSSKDFDQGHGFPSFDDA--DF 1140

BLAST of MC04g0321 vs. ExPASy TrEMBL
Match: A0A6J1C5U1 (epidermal growth factor receptor substrate 15-like 1 OS=Momordica charantia OX=3673 GN=LOC111007648 PE=4 SV=1)

HSP 1 Score: 2086 bits (5405), Expect = 0.0
Identity = 1085/1118 (97.05%), Postives = 1085/1118 (97.05%), Query Frame = 0

Query: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60
            MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI
Sbjct: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60

Query: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120
            GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120

Query: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180
            ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG
Sbjct: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180

Query: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240
            FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT
Sbjct: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240

Query: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDKLSASNKTS 300
            SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDKLSASNKTS
Sbjct: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDKLSASNKTS 300

Query: 301  TSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLST 360
            TSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLST
Sbjct: 301  TSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLST 360

Query: 361  GAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLK 420
            GAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLK
Sbjct: 361  GAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLK 420

Query: 421  QVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAASN 480
            QVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAASN
Sbjct: 421  QVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAASN 480

Query: 481  FSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVLE 540
            FSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVLE
Sbjct: 481  FSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVLE 540

Query: 541  KNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEYFRTKMQ 600
            KNLVSQLSTEEQKSLNSKFQEAADAEKK         VEELEKEILDSRQKIEYFRTKMQ
Sbjct: 541  KNLVSQLSTEEQKSLNSKFQEAADAEKK---------VEELEKEILDSRQKIEYFRTKMQ 600

Query: 601  ELV------------------------DSLAKKYEEKYKQSGDVASRLTVEESTFRDIQE 660
            ELV                        DSLAKKYEEKYKQSGDVASRLTVEESTFRDIQE
Sbjct: 601  ELVLYKSRCDNRLNEISERVSSDKREVDSLAKKYEEKYKQSGDVASRLTVEESTFRDIQE 660

Query: 661  KKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTELP 720
            KKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTELP
Sbjct: 661  KKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTELP 720

Query: 721  FGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVDS 780
            FGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVDS
Sbjct: 721  FGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVDS 780

Query: 781  QKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAPNS 840
            QKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAPNS
Sbjct: 781  QKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAPNS 840

Query: 841  PFASSAIGSPKESMDSNFGKAAGFDASPRDKDAHSDHGGVGSVFSGDKSYDEPAWGRFDT 900
            PFASSAIGSPKESMDSNFGKAAGFDASPRDKDAHSDHGGVGSVFSGDKSYDEPAWGRFDT
Sbjct: 841  PFASSAIGSPKESMDSNFGKAAGFDASPRDKDAHSDHGGVGSVFSGDKSYDEPAWGRFDT 900

Query: 901  NDDVDSVWGFNAGGSTKTDNDLNRDSYFFDSGELGLNPIRTDPFPAKRSTFAFDESVPST 960
            NDDVDSVWGFNAGGSTKTDNDLNRDSYFFDSGELGLNPIRTDPFPAKRSTFAFDESVPST
Sbjct: 901  NDDVDSVWGFNAGGSTKTDNDLNRDSYFFDSGELGLNPIRTDPFPAKRSTFAFDESVPST 960

Query: 961  PLFNSGNSPHNFREGSEPSFDSFLRFDSFGMHDSGIFPPQDNTFSRFDSMRSSRDFDQGH 1020
            PLFNSGNSPHNFREGSEPSFDSFLRFDSFGMHDSGIFPPQDNTFSRFDSMRSSRDFDQGH
Sbjct: 961  PLFNSGNSPHNFREGSEPSFDSFLRFDSFGMHDSGIFPPQDNTFSRFDSMRSSRDFDQGH 1020

Query: 1021 GFSSFGPPDTPHSSRDFNRGHGPSSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQG 1080
            GFSSFGPPDTPHSSRDFNRGHGPSSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQG
Sbjct: 1021 GFSSFGPPDTPHSSRDFNRGHGPSSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQG 1080

Query: 1081 HGFPSFDDAADPFGATGPFRASLDNQTPKRGSDNWSAF 1094
            HGFPSFDDAADPFGATGPFRASLDNQTPKRGSDNWSAF
Sbjct: 1081 HGFPSFDDAADPFGATGPFRASLDNQTPKRGSDNWSAF 1109

BLAST of MC04g0321 vs. ExPASy TrEMBL
Match: A0A6J1GLI1 (epidermal growth factor receptor substrate 15-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111455055 PE=4 SV=1)

HSP 1 Score: 1678 bits (4346), Expect = 0.0
Identity = 910/1167 (77.98%), Postives = 964/1167 (82.60%), Query Frame = 0

Query: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60
            MASAQN APNVDLFDAYFRRADLDRDGRISGAEAV+FFQGSGL K VL QIWAL+DPRQI
Sbjct: 1    MASAQNPAPNVDLFDAYFRRADLDRDGRISGAEAVSFFQGSGLPKPVLFQIWALSDPRQI 60

Query: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120
            GFLGRAEFYNALRLVTVAQSKRELTP+IVKAAL+SPAA+KIPAPQIN N QPA QFNSTA
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPDIVKAALFSPAASKIPAPQINFNTQPAPQFNSTA 120

Query: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180
             +  TP SGI  PTPS+S+GL  QVPRNV  N+P V SRE Q VRPPLATSNSAFRPAQG
Sbjct: 121  PVS-TPLSGIGTPTPSRSSGLELQVPRNVTTNVPLVSSRESQLVRPPLATSNSAFRPAQG 180

Query: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240
            F G GT SGPPPTNS IS DW S+ A G+QGT SQPPNRGVSPAG QVG FGQSSAGLTT
Sbjct: 181  FSGVGTVSGPPPTNSIISNDWVSERASGIQGTPSQPPNRGVSPAGMQVG-FGQSSAGLTT 240

Query: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDK-LSASNKT 300
            S P RPQSAPG   + +SP ESKVQGISGNGTASG YFG D F ATPV+S + + A NKT
Sbjct: 241  SQPQRPQSAPGVMPAASSPVESKVQGISGNGTASGSYFGRDAFPATPVSSKQDVPAGNKT 300

Query: 301  STSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLS 360
            STSVT PVSSV QPI RA+SLDSLQNSFMKPPL NQ+LRNQP  KPNQQ I+QPASSGLS
Sbjct: 301  STSVTVPVSSVTQPIVRATSLDSLQNSFMKPPLANQALRNQPHVKPNQQSILQPASSGLS 360

Query: 361  TGAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVL 420
            TG  NSV GQSQRPWPRMTQ DVQKYTKVFVEVDKDRDGKIT +EARNLFLSWRLPREVL
Sbjct: 361  TGLQNSVYGQSQRPWPRMTQNDVQKYTKVFVEVDKDRDGKITAQEARNLFLSWRLPREVL 420

Query: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAAS 480
             QVWDLSDQDNDSMLSVREFCIALYLLERHREGH+LPAMLPSNIMFDF SNGHPVTPAAS
Sbjct: 421  MQVWDLSDQDNDSMLSVREFCIALYLLERHREGHVLPAMLPSNIMFDFPSNGHPVTPAAS 480

Query: 481  NFSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVL 540
            N SNAAWR TAG+QQHQG+PGSGN HGAPT GV+PP PAA    EDEP TN+PKSKVP+L
Sbjct: 481  NHSNAAWRTTAGHQQHQGVPGSGNLHGAPTIGVRPPIPAAALPVEDEPQTNQPKSKVPLL 540

Query: 541  EKNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEYFRTKM 600
            +KNLVSQLSTEEQ SLNSKFQEA DAEKK         VEELEKEILDSRQKIEY+RTKM
Sbjct: 541  DKNLVSQLSTEEQNSLNSKFQEAEDAEKK---------VEELEKEILDSRQKIEYYRTKM 600

Query: 601  QEL------------------------VDSLAKKYEEKYKQSGDVASRLTVEESTFRDIQ 660
            QEL                        V+SLAKKYEEKYKQSGDVASRLTVEE+TFRDIQ
Sbjct: 601  QELILYKSRCDNRLNEISERVSSEKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQ 660

Query: 661  EKKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTEL 720
            EKKME+Y  IVKM QD   DGVLQARADRIQSDIEELVK LNERCKSYGLRAKPITLTEL
Sbjct: 661  EKKMELYHTIVKMEQDISEDGVLQARADRIQSDIEELVKVLNERCKSYGLRAKPITLTEL 720

Query: 721  PFGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVD 780
            PFGWQPGIQVGAADWDEDWDKFE+EGF+VVKELTLDVQNVIAPPKQKSKSVQKEKG+ VD
Sbjct: 721  PFGWQPGIQVGAADWDEDWDKFEEEGFSVVKELTLDVQNVIAPPKQKSKSVQKEKGSTVD 780

Query: 781  SQKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAPN 840
            SQ V PA DAD KE    GGS PD DTKGEKPPS+DETAVENGS HDNKSE  SAKSAPN
Sbjct: 781  SQNVTPAADADAKE----GGSAPDADTKGEKPPSIDETAVENGSAHDNKSENGSAKSAPN 840

Query: 841  SPFA------------------------------SSAIGSPKESMDSNFGKAAGFDASPR 900
            SPFA                              SS IGSPKE MDS+FGKAAGFDASPR
Sbjct: 841  SPFAPKSAPSSPFATKSAPSSPFAPKSAPSSPFASSVIGSPKEYMDSSFGKAAGFDASPR 900

Query: 901  DKDAHSDHGGVGSVFSGDKSYDEPAWGRFDTNDDVDSVWGFNAGGSTKTDNDLNRDSYFF 960
             KDA SDHGG GSVFSGDKSYDEPAWG FD NDD+DSVWGFNAGGSTK DND+ RD+Y+F
Sbjct: 901  AKDALSDHGGAGSVFSGDKSYDEPAWGAFDANDDIDSVWGFNAGGSTKADNDVTRDNYYF 960

Query: 961  DSGELGLNPIRTDPFPAKRSTFAFDESVPSTPLFNSGNSPHNFREGSEPSFDSFLRFDSF 1020
            DSG+ GLNPIRTDPF AKRSTFAFDESVPSTPL NSGNSP+N+ +GSEPSFDSF RFDS 
Sbjct: 961  DSGDFGLNPIRTDPFQAKRSTFAFDESVPSTPLVNSGNSPNNYHDGSEPSFDSFSRFDSS 1020

Query: 1021 GMHDSGIFPPQDNTFSRFDSMRSSRDFDQGHGFSSFGPPDTPHSSRDFNRG--------- 1080
             +HDSG FPP+D TF+RFDSMRSSRDFDQG GFSSF   DT +++RDF++G         
Sbjct: 1021 SVHDSGFFPPKD-TFTRFDSMRSSRDFDQGPGFSSFSQFDTTYNNRDFDQGGSSSLTRFD 1080

Query: 1081 ---------HGPSSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQGHGFPSFDDAAD 1094
                      G  SLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQGHGFPSFDD  D
Sbjct: 1081 SMRSSKDFDQGSPSLSRFDSMRSSKDFDQGFPSLARFDSMRSSKDFDQGHGFPSFDDP-D 1140

BLAST of MC04g0321 vs. ExPASy TrEMBL
Match: A0A1S3BIC5 (epidermal growth factor receptor substrate 15-like 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490209 PE=4 SV=1)

HSP 1 Score: 1677 bits (4342), Expect = 0.0
Identity = 913/1159 (78.77%), Postives = 968/1159 (83.52%), Query Frame = 0

Query: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60
            MASAQN APNVDLFDAYFRRADLDRDGRISGAEAV+FFQGSGL KQVLAQIW+L+DPRQI
Sbjct: 1    MASAQNPAPNVDLFDAYFRRADLDRDGRISGAEAVSFFQGSGLPKQVLAQIWSLSDPRQI 60

Query: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120
            GFLGRAEFYNALRLVTVAQSKRELTP+IVKAAL+SPAAAKIPAPQIN N QPASQFNSTA
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPDIVKAALFSPAAAKIPAPQINFNAQPASQFNSTA 120

Query: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180
            A+P TPQSG+VA TPS S+G          AN+PPV SRE QSVRPPLA  NSAFRPAQG
Sbjct: 121  AVP-TPQSGVVAQTPSPSSG----------ANVPPVSSRENQSVRPPLAAPNSAFRPAQG 180

Query: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240
            FPG G  SGPPPTNSSIS DW S+ A GVQGT SQPPNRGVSPAGTQVG FGQSSAGLT 
Sbjct: 181  FPGVGAVSGPPPTNSSISNDWVSERASGVQGTPSQPPNRGVSPAGTQVG-FGQSSAGLTA 240

Query: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDK-LSASNKT 300
            SLPPRPQSAPG   +T SP ESKVQGI+GNGT SG YFG D F ATPV+S + + A NKT
Sbjct: 241  SLPPRPQSAPGVTPATPSPLESKVQGITGNGTVSGSYFGRDAFGATPVSSKQDVPAGNKT 300

Query: 301  STSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLS 360
            STSV  PVSSV QPI RASSLDSLQ+SFMKPPL NQ+ RNQ +GK NQQ ++Q ASS LS
Sbjct: 301  STSVAVPVSSVTQPIVRASSLDSLQSSFMKPPLANQAQRNQALGKSNQQSVLQSASSVLS 360

Query: 361  TGAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVL 420
             G+ NSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITG+EARNLFLSWRLPREVL
Sbjct: 361  AGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGQEARNLFLSWRLPREVL 420

Query: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAAS 480
            KQVWDLSDQDNDSMLSVREFCIALYLLERHREGH+LPAMLPSNIMFDFSSNGHPVTPAAS
Sbjct: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHVLPAMLPSNIMFDFSSNGHPVTPAAS 480

Query: 481  NFSNAAWRP-TAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPV 540
            N+SNA WRP TAGYQQHQG+PGSGN  GAPT G +PP PA  S  E E  T++PKSKVPV
Sbjct: 481  NYSNAGWRPPTAGYQQHQGVPGSGNLQGAPTVGGRPPIPATASPVEGEQQTSQPKSKVPV 540

Query: 541  LEKNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEYFRTK 600
            LEKNL+SQLSTEEQ SLNSKFQEAADAEKK         VEELEKEIL+SRQKIEY+RTK
Sbjct: 541  LEKNLISQLSTEEQNSLNSKFQEAADAEKK---------VEELEKEILESRQKIEYYRTK 600

Query: 601  MQELV------------------------DSLAKKYEEKYKQSGDVASRLTVEESTFRDI 660
            MQELV                        +SLAKKYEEKYKQSGDVASRLTVEE+TFRDI
Sbjct: 601  MQELVLYKSRCDNRLNEISERVSSDKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDI 660

Query: 661  QEKKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTE 720
            QEKKME+YQAIVKM QDG ADGVLQARADRIQSDIEELVK LNERCKSYGLRAKPITLTE
Sbjct: 661  QEKKMELYQAIVKMEQDGSADGVLQARADRIQSDIEELVKSLNERCKSYGLRAKPITLTE 720

Query: 721  LPFGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAV 780
            LPFGWQPGIQVGAADWDEDWDKFEDEGF+VVKELTLDVQNVIAPPKQKSKSVQK K   V
Sbjct: 721  LPFGWQPGIQVGAADWDEDWDKFEDEGFSVVKELTLDVQNVIAPPKQKSKSVQKGK---V 780

Query: 781  DSQKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAP 840
            DSQ V PA D DTKE    G S P+ DTK +KPPSMDE AVENGS HDNKSE  SAKSAP
Sbjct: 781  DSQNVTPAADDDTKE----GDSAPNADTKRDKPPSMDEAAVENGSAHDNKSEDGSAKSAP 840

Query: 841  NSPFA--------------------SSAIGSPKESMDSNFGKAAGFDASPRDKDAHSDHG 900
            NSPFA                    SS IGSPKE MDS+FGK AGFD+SPRDKDA SDHG
Sbjct: 841  NSPFALKSAPGSPFAPKSAPGSPFASSIIGSPKEYMDSHFGKTAGFDSSPRDKDALSDHG 900

Query: 901  GVGSVFSGDKSYDEPAWGRFDTNDDVDSVWGFNAGGSTKTDNDLNRDSYFFDSGELGLNP 960
            G GSVFSGDKSYDEPAWG FD NDD+DSVWGFNAGGSTKTDND+NRD+YFFDSG+LGLNP
Sbjct: 901  GAGSVFSGDKSYDEPAWGTFDANDDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNP 960

Query: 961  IRTDPFPAKRSTFAFDESVPSTPLFNSGNSPHNFREGSEPSFDSFLRFDSFGMHDSGIFP 1020
            IRTDPF AKRSTFAFDESVPSTPLFNSGNSPHN+ EGSE +FDSF RFD+  +HDSG FP
Sbjct: 961  IRTDPFQAKRSTFAFDESVPSTPLFNSGNSPHNYHEGSEANFDSFSRFDTSSVHDSGFFP 1020

Query: 1021 PQDNTFSRFDSMRSSRDFDQGHGFSSFGPPDTPHSSRDFNRGHGPSSLSRFDSMRSSKDF 1080
            P++ TFSRFDSMRSSRDFDQG GFSSFG  DT HSSRDF++  GPSSL+RFDSMRSSKDF
Sbjct: 1021 PRE-TFSRFDSMRSSRDFDQGSGFSSFGQFDTTHSSRDFDQS-GPSSLTRFDSMRSSKDF 1080

Query: 1081 DQGFPSLARFDSM-------------------RSSKDFDQGHGFPSFDDAADPFGATGPF 1094
            DQGFPSL+RFDSM                   RSSKDFDQGHGFPSFDD  DPFG+TGPF
Sbjct: 1081 DQGFPSLSRFDSMQSSKDFDQGFPSFSRFDSMRSSKDFDQGHGFPSFDDP-DPFGSTGPF 1128

BLAST of MC04g0321 vs. ExPASy TrEMBL
Match: A0A5D3DI91 (Epidermal growth factor receptor substrate 15-like 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G003400 PE=4 SV=1)

HSP 1 Score: 1673 bits (4332), Expect = 0.0
Identity = 913/1169 (78.10%), Postives = 968/1169 (82.81%), Query Frame = 0

Query: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60
            MASAQN APNVDLFDAYFRRADLDRDGRISGAEAV+FFQGSGL KQVLAQIW+L+DPRQI
Sbjct: 1    MASAQNPAPNVDLFDAYFRRADLDRDGRISGAEAVSFFQGSGLPKQVLAQIWSLSDPRQI 60

Query: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120
            GFLGRAEFYNALRLVTVAQSKRELTP+IVKAAL+SPAAAKIPAPQIN N QPASQFNSTA
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPDIVKAALFSPAAAKIPAPQINFNAQPASQFNSTA 120

Query: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180
            A+P TPQSG+VA TPS S+G          AN+PPV SRE QSVRPPLA  NSAFRPAQG
Sbjct: 121  AVP-TPQSGVVAQTPSPSSG----------ANVPPVSSRENQSVRPPLAAPNSAFRPAQG 180

Query: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240
            FPG G  SGPPPTNSSIS DW S+ A GVQGT SQPPNRGVSPAGTQVG FGQSSAGLT 
Sbjct: 181  FPGVGAVSGPPPTNSSISNDWVSERASGVQGTPSQPPNRGVSPAGTQVG-FGQSSAGLTA 240

Query: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDK-LSASNKT 300
            SLPPRPQSAPG   +T SP ESKVQGI+GNGT SG YFG D F ATPV+S + + A NKT
Sbjct: 241  SLPPRPQSAPGVTPATPSPLESKVQGITGNGTVSGSYFGRDAFGATPVSSKQDVPAGNKT 300

Query: 301  STSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLS 360
            STSV  PVSSV QPI RASSLDSLQ+SFMKPPL NQ+ RNQ +GK NQQ ++Q ASS LS
Sbjct: 301  STSVAVPVSSVTQPIVRASSLDSLQSSFMKPPLANQAQRNQALGKSNQQSVLQSASSVLS 360

Query: 361  TGAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVL 420
             G+ NSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITG+EARNLFLSWRLPREVL
Sbjct: 361  AGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGQEARNLFLSWRLPREVL 420

Query: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAAS 480
            KQVWDLSDQDNDSMLSVREFCIALYLLERHREGH+LPAMLPSNIMFDFSSNGHPVTPAAS
Sbjct: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHVLPAMLPSNIMFDFSSNGHPVTPAAS 480

Query: 481  NFSNAAWRP-TAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPV 540
            N+SNA WRP TAGYQQHQG+PGSGN  GAPT G +PP PA  S  E E  T++PKSKVPV
Sbjct: 481  NYSNAGWRPPTAGYQQHQGVPGSGNLQGAPTVGGRPPIPATASPVEGEQQTSQPKSKVPV 540

Query: 541  LEKNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEYFRTK 600
            LEKNL+SQLSTEEQ SLNSKFQEAADAEKK         VEELEKEIL+SRQKIEY+RTK
Sbjct: 541  LEKNLISQLSTEEQNSLNSKFQEAADAEKK---------VEELEKEILESRQKIEYYRTK 600

Query: 601  MQELV------------------------DSLAKKYEEKYKQSGDVASRLTVEESTFRDI 660
            MQELV                        +SLAKKYEEKYKQSGDVASRLTVEE+TFRDI
Sbjct: 601  MQELVLYKSRCDNRLNEISERVSSDKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDI 660

Query: 661  QEKKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTE 720
            QEKKME+YQAIVKM QDG ADGVLQARADRIQSDIEELVK LNERCKSYGLRAKPITLTE
Sbjct: 661  QEKKMELYQAIVKMEQDGSADGVLQARADRIQSDIEELVKSLNERCKSYGLRAKPITLTE 720

Query: 721  LPFGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAV 780
            LPFGWQPGIQVGAADWDEDWDKFEDEGF+VVKELTLDVQNVIAPPKQKSKSVQK K   V
Sbjct: 721  LPFGWQPGIQVGAADWDEDWDKFEDEGFSVVKELTLDVQNVIAPPKQKSKSVQKGK---V 780

Query: 781  DSQKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAP 840
            DSQ V PA D DTKE    G S P+ DTK +KPPSMDE AVENGS HDNKSE  SAKSAP
Sbjct: 781  DSQNVTPAADDDTKE----GDSAPNADTKRDKPPSMDEAAVENGSAHDNKSEDGSAKSAP 840

Query: 841  NSPF------------------------------ASSAIGSPKESMDSNFGKAAGFDASP 900
            NSPF                              ASS IGSPKE MDS+FGK AGFD+SP
Sbjct: 841  NSPFTAKSAPNSPFALKSAPGSPFAPKSAPGSPFASSIIGSPKEYMDSHFGKTAGFDSSP 900

Query: 901  RDKDAHSDHGGVGSVFSGDKSYDEPAWGRFDTNDDVDSVWGFNAGGSTKTDNDLNRDSYF 960
            RDKDA SDHGG GSVFSGDKSYDEPAWG FD NDD+DSVWGFNAGGSTKTDND+NRD+YF
Sbjct: 901  RDKDALSDHGGAGSVFSGDKSYDEPAWGTFDANDDIDSVWGFNAGGSTKTDNDVNRDNYF 960

Query: 961  FDSGELGLNPIRTDPFPAKRSTFAFDESVPSTPLFNSGNSPHNFREGSEPSFDSFLRFDS 1020
            FDSG+LGLNPIRTDPF AKRSTFAFDESVPSTPLFNSGNSPHN+ EGSE +FDSF RFD+
Sbjct: 961  FDSGDLGLNPIRTDPFQAKRSTFAFDESVPSTPLFNSGNSPHNYHEGSEANFDSFSRFDT 1020

Query: 1021 FGMHDSGIFPPQDNTFSRFDSMRSSRDFDQGHGFSSFGPPDTPHSSRDFNRGHGPSSLSR 1080
              +HDSG FPP++ TFSRFDSMRSSRDFDQG GFSSFG  DT HSSRDF++  GPSSL+R
Sbjct: 1021 SSVHDSGFFPPRE-TFSRFDSMRSSRDFDQGSGFSSFGQFDTTHSSRDFDQS-GPSSLTR 1080

Query: 1081 FDSMRSSKDFDQGFPSLARFDSM-------------------RSSKDFDQGHGFPSFDDA 1094
            FDSMRSSKDFDQGFPSL+RFDSM                   RSSKDFDQGHGFPSFDD 
Sbjct: 1081 FDSMRSSKDFDQGFPSLSRFDSMQSSKDFDQGFPSFSRFDSMRSSKDFDQGHGFPSFDDP 1138

BLAST of MC04g0321 vs. ExPASy TrEMBL
Match: A0A1S3BHS4 (epidermal growth factor receptor substrate 15-like 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490209 PE=4 SV=1)

HSP 1 Score: 1673 bits (4332), Expect = 0.0
Identity = 913/1169 (78.10%), Postives = 968/1169 (82.81%), Query Frame = 0

Query: 1    MASAQNQAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQI 60
            MASAQN APNVDLFDAYFRRADLDRDGRISGAEAV+FFQGSGL KQVLAQIW+L+DPRQI
Sbjct: 1    MASAQNPAPNVDLFDAYFRRADLDRDGRISGAEAVSFFQGSGLPKQVLAQIWSLSDPRQI 60

Query: 61   GFLGRAEFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTA 120
            GFLGRAEFYNALRLVTVAQSKRELTP+IVKAAL+SPAAAKIPAPQIN N QPASQFNSTA
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPDIVKAALFSPAAAKIPAPQINFNAQPASQFNSTA 120

Query: 121  ALPPTPQSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQG 180
            A+P TPQSG+VA TPS S+G          AN+PPV SRE QSVRPPLA  NSAFRPAQG
Sbjct: 121  AVP-TPQSGVVAQTPSPSSG----------ANVPPVSSRENQSVRPPLAAPNSAFRPAQG 180

Query: 181  FPGAGTASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGTQVGAFGQSSAGLTT 240
            FPG G  SGPPPTNSSIS DW S+ A GVQGT SQPPNRGVSPAGTQVG FGQSSAGLT 
Sbjct: 181  FPGVGAVSGPPPTNSSISNDWVSERASGVQGTPSQPPNRGVSPAGTQVG-FGQSSAGLTA 240

Query: 241  SLPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDK-LSASNKT 300
            SLPPRPQSAPG   +T SP ESKVQGI+GNGT SG YFG D F ATPV+S + + A NKT
Sbjct: 241  SLPPRPQSAPGVTPATPSPLESKVQGITGNGTVSGSYFGRDAFGATPVSSKQDVPAGNKT 300

Query: 301  STSVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLS 360
            STSV  PVSSV QPI RASSLDSLQ+SFMKPPL NQ+ RNQ +GK NQQ ++Q ASS LS
Sbjct: 301  STSVAVPVSSVTQPIVRASSLDSLQSSFMKPPLANQAQRNQALGKSNQQSVLQSASSVLS 360

Query: 361  TGAHNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVL 420
             G+ NSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITG+EARNLFLSWRLPREVL
Sbjct: 361  AGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGQEARNLFLSWRLPREVL 420

Query: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFDFSSNGHPVTPAAS 480
            KQVWDLSDQDNDSMLSVREFCIALYLLERHREGH+LPAMLPSNIMFDFSSNGHPVTPAAS
Sbjct: 421  KQVWDLSDQDNDSMLSVREFCIALYLLERHREGHVLPAMLPSNIMFDFSSNGHPVTPAAS 480

Query: 481  NFSNAAWRP-TAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPV 540
            N+SNA WRP TAGYQQHQG+PGSGN  GAPT G +PP PA  S  E E  T++PKSKVPV
Sbjct: 481  NYSNAGWRPPTAGYQQHQGVPGSGNLQGAPTVGGRPPIPATASPVEGEQQTSQPKSKVPV 540

Query: 541  LEKNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEYFRTK 600
            LEKNL+SQLSTEEQ SLNSKFQEAADAEKK         VEELEKEIL+SRQKIEY+RTK
Sbjct: 541  LEKNLISQLSTEEQNSLNSKFQEAADAEKK---------VEELEKEILESRQKIEYYRTK 600

Query: 601  MQELV------------------------DSLAKKYEEKYKQSGDVASRLTVEESTFRDI 660
            MQELV                        +SLAKKYEEKYKQSGDVASRLTVEE+TFRDI
Sbjct: 601  MQELVLYKSRCDNRLNEISERVSSDKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDI 660

Query: 661  QEKKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTE 720
            QEKKME+YQAIVKM QDG ADGVLQARADRIQSDIEELVK LNERCKSYGLRAKPITLTE
Sbjct: 661  QEKKMELYQAIVKMEQDGSADGVLQARADRIQSDIEELVKSLNERCKSYGLRAKPITLTE 720

Query: 721  LPFGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAV 780
            LPFGWQPGIQVGAADWDEDWDKFEDEGF+VVKELTLDVQNVIAPPKQKSKSVQK K   V
Sbjct: 721  LPFGWQPGIQVGAADWDEDWDKFEDEGFSVVKELTLDVQNVIAPPKQKSKSVQKGK---V 780

Query: 781  DSQKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGESAKSAP 840
            DSQ V PA D DTKE    G S P+ DTK +KPPSMDE AVENGS HDNKSE  SAKSAP
Sbjct: 781  DSQNVTPAADDDTKE----GDSAPNADTKRDKPPSMDEAAVENGSAHDNKSEDGSAKSAP 840

Query: 841  NSPF------------------------------ASSAIGSPKESMDSNFGKAAGFDASP 900
            NSPF                              ASS IGSPKE MDS+FGK AGFD+SP
Sbjct: 841  NSPFTAKSAPNSPFALKSAPGSPFAPKSAPGSPFASSIIGSPKEYMDSHFGKTAGFDSSP 900

Query: 901  RDKDAHSDHGGVGSVFSGDKSYDEPAWGRFDTNDDVDSVWGFNAGGSTKTDNDLNRDSYF 960
            RDKDA SDHGG GSVFSGDKSYDEPAWG FD NDD+DSVWGFNAGGSTKTDND+NRD+YF
Sbjct: 901  RDKDALSDHGGAGSVFSGDKSYDEPAWGTFDANDDIDSVWGFNAGGSTKTDNDVNRDNYF 960

Query: 961  FDSGELGLNPIRTDPFPAKRSTFAFDESVPSTPLFNSGNSPHNFREGSEPSFDSFLRFDS 1020
            FDSG+LGLNPIRTDPF AKRSTFAFDESVPSTPLFNSGNSPHN+ EGSE +FDSF RFD+
Sbjct: 961  FDSGDLGLNPIRTDPFQAKRSTFAFDESVPSTPLFNSGNSPHNYHEGSEANFDSFSRFDT 1020

Query: 1021 FGMHDSGIFPPQDNTFSRFDSMRSSRDFDQGHGFSSFGPPDTPHSSRDFNRGHGPSSLSR 1080
              +HDSG FPP++ TFSRFDSMRSSRDFDQG GFSSFG  DT HSSRDF++  GPSSL+R
Sbjct: 1021 SSVHDSGFFPPRE-TFSRFDSMRSSRDFDQGSGFSSFGQFDTTHSSRDFDQS-GPSSLTR 1080

Query: 1081 FDSMRSSKDFDQGFPSLARFDSM-------------------RSSKDFDQGHGFPSFDDA 1094
            FDSMRSSKDFDQGFPSL+RFDSM                   RSSKDFDQGHGFPSFDD 
Sbjct: 1081 FDSMRSSKDFDQGFPSLSRFDSMQSSKDFDQGFPSFSRFDSMRSSKDFDQGHGFPSFDDP 1138

BLAST of MC04g0321 vs. TAIR 10
Match: AT1G20760.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 685.3 bits (1767), Expect = 8.3e-197
Identity = 492/1135 (43.35%), Postives = 634/1135 (55.86%), Query Frame = 0

Query: 7    QAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQIGFLGRA 66
            Q PN+D F+AYF+RADLD DGRISGAEAV FFQGSGL+KQVLAQIW+L+D    GFL R 
Sbjct: 4    QNPNMDQFEAYFKRADLDGDGRISGAEAVGFFQGSGLSKQVLAQIWSLSDRSHSGFLDRQ 63

Query: 67   EFYNALRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTAALPPTP 126
             FYN+LRLVTVAQSKR+LTPEIV AAL +PAAAKIP P+IN++  PA +        P P
Sbjct: 64   NFYNSLRLVTVAQSKRDLTPEIVNAALNTPAAAKIPPPKINLSAIPAPR--------PNP 123

Query: 127  QSGIVAPTPSQSTGLGSQVPRNVNANLPPVPSREGQSVRPPLATSNSAFRPAQGFPGAGT 186
             +  V P     +G G     N   N    P ++ Q +RP    S        G      
Sbjct: 124  AATTVGPV----SGFGGPGAPNAIVNQNYFPPQQNQQMRPNQGIS--------GLTSLRP 183

Query: 187  ASGPPPTNSSISTDWASDGAGGVQGTSSQPPNRGVSPAGT-----QVGAFGQSSAGLTTS 246
            A+GP    S++S  +     G V       P     P  +      + A   +++G ++ 
Sbjct: 184  AAGPEYRPSALSGQFQPVPVGSVTHPPQPVPTSVSGPGSSTFNLNSLYAGAGNTSGYSSG 243

Query: 247  LPPRPQSAPGAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSATPVTSDKLSASNKTST 306
                  +AP       S  + K   +SGN        GGD+FS+     +  + SN + +
Sbjct: 244  FGGGSLAAPSPGLKQESHIDPKALVVSGN--------GGDMFSSFQQKQEP-TLSNSSIS 303

Query: 307  SVTAPVSSVAQPIARASSLDSLQNSFMKPPLGNQSLRNQPVGKPNQQGIVQPASSGLSTG 366
            S   P S+  QP  + ++LDSLQ++F   P GNQ  + +P          Q  SSGL  G
Sbjct: 304  SAIVPTSAGIQPPTKPNALDSLQSTFSMLPSGNQLQQPRPAASSQPAVSSQGPSSGLPPG 363

Query: 367  A-----HNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPR 426
            +     H++ +G +Q PWP+M  +DVQKYTKVF+EVD D+DGKITGE+ARNLFLSWRLPR
Sbjct: 364  SAVGSGHSTPAGNNQPPWPKMKPSDVQKYTKVFMEVDSDKDGKITGEQARNLFLSWRLPR 423

Query: 427  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNIMFD---FSSNGHP 486
            EVLK VW+LSDQDND+MLS+REFCI+LYL+ER+REG  LP  LPS+IMFD    S +G P
Sbjct: 424  EVLKHVWELSDQDNDTMLSLREFCISLYLMERYREGRPLPTALPSSIMFDETLLSISGAP 483

Query: 487  VTPAASNFSNAAWRPTAGYQQHQGIPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPK 546
                +  ++NA W    G+ Q    PG G R   PT G++PP PA   Q       N+P+
Sbjct: 484  ----SHGYANAGWGSGQGFVQQ---PGMGARPITPTTGMRPPVPAPGPQPGSGIPPNQPR 543

Query: 547  SKVPVLEKNLVSQLSTEEQKSLNSKFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIE 606
            ++ PVL+    + L      S N   + AAD EK          V+E +   +DSR+K++
Sbjct: 544  NQAPVLDAPFANHLGNGYSASSNLP-EAAADEEK----------VDEKQNAYMDSREKLD 603

Query: 607  YFRTKMQELV------------------------DSLAKKYEEKYKQSGDVASRLTVEES 666
            Y+RTKMQ++V                        ++LAKKYEEKYKQ  ++ S+LT+EE+
Sbjct: 604  YYRTKMQDIVLYKSRCDNRLNEISERASADKREAETLAKKYEEKYKQVAEIGSKLTIEEA 663

Query: 667  TFRDIQEKKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKP 726
             FR+I+ +KME+ QAIV M Q G ADG+LQ RADRIQSD+EEL+K L ERCK +GL    
Sbjct: 664  RFREIEGRKMELSQAIVNMEQGGSADGLLQVRADRIQSDLEELMKALTERCKKHGLEVNS 723

Query: 727  ITLTELPFGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKE 786
              L +LP GWQPGIQ GAA WDE+WDKFEDEGF    E+T D        K+++ S +KE
Sbjct: 724  KALVDLPAGWQPGIQEGAALWDEEWDKFEDEGFG--NEITFD------KSKEQNSSGEKE 783

Query: 787  KGAAVDSQKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNKSEGES 846
             G   D    +  PD+ T      G      D   E          E G+TH   SE  S
Sbjct: 784  NGTVDDG---SGPPDSPTHVEDNYGPFSETSDRHHES---------EYGTTH---SEDRS 843

Query: 847  AKSAPNSPFASSAIGSPKESMDSNFGKAAGFDASPRDKDAHSDHGGVGSVFSGDKSYDEP 906
             + +P S  A+  + SP  S   N                              + +D+ 
Sbjct: 844  PRDSPVSRNATE-VPSPDYSQGKN-----------------------------SEFFDDS 903

Query: 907  AW-GRFDTNDDVDSVWGFNAGGSTKTDNDLNRDSYFFDSGELGLNPIRTD-----PFPAK 966
             W   FDTNDDVDSVWGF+A  S   D       YF    + G N  R D      F A+
Sbjct: 904  NWASAFDTNDDVDSVWGFDASKSQDGD-------YFGSGDDFGGNTARADSPSSRSFGAQ 963

Query: 967  R-STFAFDESVPSTPLFNSGNSPHNFREGS--EPSFDSFLRFDSFGMHDSGI-FPPQDNT 1026
            R S FAFD+SVPSTPL   GNSP  F + S  + +FDSF RFDSF   ++G  F  Q   
Sbjct: 964  RKSQFAFDDSVPSTPLSRFGNSPPRFSDASARDNNFDSFSRFDSFNTSEAGAGFSSQPER 1019

Query: 1027 FSRFDSMRSSRDFDQGHGFSSFGPPDTPHSSRDFNRGHGPSSLSRFDSMRSSKDFDQGFP 1086
             SRFDS+ SS+DF  G  FS F   D+ +SSRD     G    SRFDS+ SSKDF  G P
Sbjct: 1024 LSRFDSINSSKDFG-GAAFSRF---DSINSSRDVT---GAEKFSRFDSINSSKDF--GGP 1019

Query: 1087 SLARFDSMRSSKDFDQGHGFPSFDDAADPFGATGPFRASLDNQTPKRGSDNWSAF 1095
            SL+RFDSM S+KDF   HG+ SFDD ADPFG+TGPF+ S D ++P + SDNW++F
Sbjct: 1084 SLSRFDSMNSTKDFSGSHGY-SFDD-ADPFGSTGPFKVSSD-ESPTKRSDNWNSF 1019

BLAST of MC04g0321 vs. TAIR 10
Match: AT1G21630.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 654.8 bits (1688), Expect = 1.2e-187
Identity = 508/1235 (41.13%), Postives = 654/1235 (52.96%), Query Frame = 0

Query: 12   DLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQIGFLGRAEFYNA 71
            DLFD YFRRADLD DG ISGAEAVAFFQGS L K VLAQ+W+ AD ++ G+LGRAEFYNA
Sbjct: 11   DLFDTYFRRADLDGDGHISGAEAVAFFQGSNLPKHVLAQVWSYADSKKAGYLGRAEFYNA 70

Query: 72   LRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTAALPPTPQSGIV 131
            L+LVTVAQS+RELT EIVKAA+YSPA+A IPAP+IN+   P+ Q      LP T   G+ 
Sbjct: 71   LKLVTVAQSRRELTAEIVKAAIYSPASANIPAPKINLAATPSPQ--PRGVLPATQAQGVT 130

Query: 132  APTPSQSTGL-GSQVPRNVN-ANLPPVPSREGQSVRPPLATSNSAFRPAQGFPGAGTASG 191
            +  PS + G+ G  +   V+ +N   VP ++ Q    P + +   F+ + G P  GT + 
Sbjct: 131  S-MPSVAAGVRGPHMGGTVSTSNQQVVPGQQNQFTGIPPSQTQQNFQ-SPGMPAGGT-NA 190

Query: 192  PPPTNSSISTDWASDGAGGVQG-TSSQPPNR----GVSPAGTQVGAFGQS--SAGLTTSL 251
            P P N  + +DW S  + G  G  +SQ P+     G++   +      +   +  +T+S 
Sbjct: 191  PRPANQPMPSDWLSGRSVGPSGNVNSQIPSSQSTYGLTAPNSTANHITKPHITPAVTSST 250

Query: 252  PPRPQ-SAP----------------GAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSA 311
              RPQ SAP                   S+   P + K    SGNG  S   F GD+FS 
Sbjct: 251  TTRPQESAPVHNPQESSATFGSRVSNVPSNQLVPKDPKELAASGNGFTSDSLF-GDVFSV 310

Query: 312  T---PVTSDKLSASNKTSTSVTAPV---SSVAQPIARASSLDSLQNSFMKPPLGNQSLRN 371
            T   P      SAS    +SVT        + Q + R SS+   Q S  +  +G Q+   
Sbjct: 311  TSTQPKQHPTGSASTTGISSVTTGTVAGPEITQSVVRQSSIPQ-QGSLSQHAVGVQTQLT 370

Query: 372  QPVGKPNQQGIVQPASSGLSTGAHNSVS-------------------GQSQRPWPRMTQT 431
               G+P           G + G   S +                   GQSQ PWP+MT  
Sbjct: 371  GNSGQPYTSSGAASGPPGSTVGVGISATSQLAQRPPHPHSQPQPRPQGQSQPPWPKMTPA 430

Query: 432  DVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLKQVWDLSDQDNDSMLSVREFC 491
            DVQKYTKVFV+VD DRDGKITG +ARNLFLSWRLPR+ LKQVWDLSDQDNDSMLS+REFC
Sbjct: 431  DVQKYTKVFVQVDTDRDGKITGNQARNLFLSWRLPRDALKQVWDLSDQDNDSMLSLREFC 490

Query: 492  IALYLLERHREGHILPAMLPSNIMFD---FSSNGHPVTPAASNFSNAAWRPTAGYQQHQG 551
            IA+YL+ER+REG  LP + PS+I+     F+S G  V P      NA+W    G+QQ Q 
Sbjct: 491  IAVYLMERYREGRPLPPVFPSSIIHSESMFTSPGQSVAP----HGNASWGHPQGFQQ-QP 550

Query: 552  IPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVLEKNLVSQLSTEEQKSLNS 611
             PG G R  A   G +PP+P  +S  +      +PK K+PVLEK LV QLS EEQ SLN+
Sbjct: 551  HPG-GLRPPAGPKG-KPPRPVPLSPSDGMVQPTQPKRKMPVLEKPLVDQLSKEEQDSLNT 610

Query: 612  KFQEAADAEKKAPLLLFNALVEELEKEILDSRQKIEYFRTKMQELV-------------- 671
            KF+EA   +KK         V+ELEKEI DS+QKI++FR KMQELV              
Sbjct: 611  KFEEATAVDKK---------VDELEKEIADSKQKIDFFRAKMQELVLYKSRCDNRYNEIA 670

Query: 672  ----------DSLAKKYEEKYKQSGDVASRLTVEESTFRDIQEKKMEVYQAIVKMGQDGG 731
                      +SLAKKYEEKYK+SG+V S+LT+EE+TFRDIQEKKME+YQAIVK  +   
Sbjct: 671  ERVLGDKRELESLAKKYEEKYKKSGNVGSKLTIEEATFRDIQEKKMELYQAIVKFEEGKL 730

Query: 732  ADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITLTELPFGWQPGIQVGAADWDED 791
             D +++ R + IQS +EEL+K LNERCK YG+R KP +L ELPFGWQPGIQ GAADWDED
Sbjct: 731  DDSIVKERTEHIQSGLEELIKNLNERCKQYGVRGKPTSLVELPFGWQPGIQEGAADWDED 790

Query: 792  WDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGAAVDSQKVAPAPDADTKEGTKE 851
            WDK EDEGFT VKELTLD+QNVIAPPK+KS + +KE   +    +     DAD+K G K+
Sbjct: 791  WDKLEDEGFTFVKELTLDIQNVIAPPKEKSSAWRKEVDVSSKEGEDVSFSDADSKTGKKQ 850

Query: 852  GGSEPDPDTKGEKPPSMDETAVENGSTHDNK-SEGESAKSAPNSPFASSAIGSPKESMDS 911
               E D +    K   +D    +NGS  D+K  +G  A S+P +    S  G       +
Sbjct: 851  SSGEEDSEQSEGKTSDVD-ARDKNGSLDDSKVRKGIEADSSPRTKDTRSENGHDDGESTA 910

Query: 912  NFGKAAGFDASPR---------DKDAHSDHGGVGSVFSGDKSYDEPAWGRFDTNDDV--- 971
            + GK   +D+            D     DHG   S F     +D+ +     T   +   
Sbjct: 911  SAGKTVNYDSHDETDSVSSVNPDNGKDKDHGKYDSGFGFGFGFDDFSIKPIKTGSTISND 970

Query: 972  ----------DSVWG--FNAGGSTKTDNDLNRDSY----------------FFDSGELGL 1031
                      DSV     NA   + T   L  DS                 FFD      
Sbjct: 971  FLPPKLSIFADSVPSPPANASDVSPTKPSLFADSVPSTPATNNASYPGQKSFFDDSVPST 1030

Query: 1032 NPIRTDPFPAKRSTF--------------------AFDESVPSTPLFNSGNSPHNFREGS 1091
                 + F  K+S F                     FD+SVPSTP +++ +         
Sbjct: 1031 PAYPGNLFAEKKSFFDDSVPSTPAYPGNLFAEKKSYFDDSVPSTPAYSTSDFGGKPFASE 1090

Query: 1092 EPSFDSFLRFDSFGMHDSGIFPP------QDNTFSRFDSMRSSRD----FDQGHGFSSFG 1095
             P  D+     S  M DS    P       +N+FSRFDS  S+ +      +     S  
Sbjct: 1091 TPRSDNLFPGRSPFMFDSVPSTPAAHDDFSNNSFSRFDSFNSNNNDAFSLSRTDSMRSTS 1150

BLAST of MC04g0321 vs. TAIR 10
Match: AT1G21630.2 (Calcium-binding EF hand family protein )

HSP 1 Score: 652.5 bits (1682), Expect = 5.9e-187
Identity = 511/1256 (40.68%), Postives = 658/1256 (52.39%), Query Frame = 0

Query: 12   DLFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQIGFLGRAEFYNA 71
            DLFD YFRRADLD DG ISGAEAVAFFQGS L K VLAQ+W+ AD ++ G+LGRAEFYNA
Sbjct: 11   DLFDTYFRRADLDGDGHISGAEAVAFFQGSNLPKHVLAQVWSYADSKKAGYLGRAEFYNA 70

Query: 72   LRLVTVAQSKRELTPEIVKAALYSPAAAKIPAPQINVNIQPASQFNSTAALPPTPQSGIV 131
            L+LVTVAQS+RELT EIVKAA+YSPA+A IPAP+IN+   P+ Q      LP T   G+ 
Sbjct: 71   LKLVTVAQSRRELTAEIVKAAIYSPASANIPAPKINLAATPSPQ--PRGVLPATQAQGVT 130

Query: 132  APTPSQSTGL-GSQVPRNVN-ANLPPVPSREGQSVRPPLATSNSAFRPAQGFPGAGTASG 191
            +  PS + G+ G  +   V+ +N   VP ++ Q    P + +   F+ + G P  GT + 
Sbjct: 131  S-MPSVAAGVRGPHMGGTVSTSNQQVVPGQQNQFTGIPPSQTQQNFQ-SPGMPAGGT-NA 190

Query: 192  PPPTNSSISTDWASDGAGGVQG-TSSQPPNR----GVSPAGTQVGAFGQS--SAGLTTSL 251
            P P N  + +DW S  + G  G  +SQ P+     G++   +      +   +  +T+S 
Sbjct: 191  PRPANQPMPSDWLSGRSVGPSGNVNSQIPSSQSTYGLTAPNSTANHITKPHITPAVTSST 250

Query: 252  PPRPQ-SAP----------------GAKSSTTSPAESKVQGISGNGTASGPYFGGDIFSA 311
              RPQ SAP                   S+   P + K    SGNG  S   F GD+FS 
Sbjct: 251  TTRPQESAPVHNPQESSATFGSRVSNVPSNQLVPKDPKELAASGNGFTSDSLF-GDVFSV 310

Query: 312  T---PVTSDKLSASNKTSTSVTAPV---SSVAQPIARASSLDSLQNSFMKPPLGNQSLRN 371
            T   P      SAS    +SVT        + Q + R SS+   Q S  +  +G Q+   
Sbjct: 311  TSTQPKQHPTGSASTTGISSVTTGTVAGPEITQSVVRQSSIPQ-QGSLSQHAVGVQTQLT 370

Query: 372  QPVGKPNQQGIVQPASSGLSTGAHNSVS-------------------GQSQRPWPRMTQT 431
               G+P           G + G   S +                   GQSQ PWP+MT  
Sbjct: 371  GNSGQPYTSSGAASGPPGSTVGVGISATSQLAQRPPHPHSQPQPRPQGQSQPPWPKMTPA 430

Query: 432  DVQKYTKVFVEVDKDRDGKITGEEARNLFLSWRLPREVLKQVWDLSDQDNDSMLSVREFC 491
            DVQKYTKVFV+VD DRDGKITG +ARNLFLSWRLPR+ LKQVWDLSDQDNDSMLS+REFC
Sbjct: 431  DVQKYTKVFVQVDTDRDGKITGNQARNLFLSWRLPRDALKQVWDLSDQDNDSMLSLREFC 490

Query: 492  IALYLLERHREGHILPAMLPSNIMFD---FSSNGHPVTPAASNFSNAAWRPTAGYQQHQG 551
            IA+YL+ER+REG  LP + PS+I+     F+S G  V P      NA+W    G+QQ Q 
Sbjct: 491  IAVYLMERYREGRPLPPVFPSSIIHSESMFTSPGQSVAP----HGNASWGHPQGFQQ-QP 550

Query: 552  IPGSGNRHGAPTAGVQPPKPAAVSQFEDEPHTNKPKSKVPVLEKNLVSQLSTEEQKSLNS 611
             PG G R  A   G +PP+P  +S  +      +PK K+PVLEK LV QLS EEQ SLN+
Sbjct: 551  HPG-GLRPPAGPKG-KPPRPVPLSPSDGMVQPTQPKRKMPVLEKPLVDQLSKEEQDSLNT 610

Query: 612  KFQEAADAEKKAPLLLFN---------------------ALVEELEKEILDSRQKIEYFR 671
            KF+EA   +KK     F+                     A+V+ELEKEI DS+QKI++FR
Sbjct: 611  KFEEATAVDKKFSFFAFSIRRYLLPLPYGIYMKYLWICVAIVDELEKEIADSKQKIDFFR 670

Query: 672  TKMQELV------------------------DSLAKKYEEKYKQSGDVASRLTVEESTFR 731
             KMQELV                        +SLAKKYEEKYK+SG+V S+LT+EE+TFR
Sbjct: 671  AKMQELVLYKSRCDNRYNEIAERVLGDKRELESLAKKYEEKYKKSGNVGSKLTIEEATFR 730

Query: 732  DIQEKKMEVYQAIVKMGQDGGADGVLQARADRIQSDIEELVKGLNERCKSYGLRAKPITL 791
            DIQEKKME+YQAIVK  ++G  D  +  R + IQS +EEL+K LNERCK YG+R KP +L
Sbjct: 731  DIQEKKMELYQAIVKF-EEGKLDDSIVKRTEHIQSGLEELIKNLNERCKQYGVRGKPTSL 790

Query: 792  TELPFGWQPGIQVGAADWDEDWDKFEDEGFTVVKELTLDVQNVIAPPKQKSKSVQKEKGA 851
             ELPFGWQPGIQ GAADWDEDWDK EDEGFT VKELTLD+QNVIAPPK+KS + +KE   
Sbjct: 791  VELPFGWQPGIQEGAADWDEDWDKLEDEGFTFVKELTLDIQNVIAPPKEKSSAWRKEVDV 850

Query: 852  AVDSQKVAPAPDADTKEGTKEGGSEPDPDTKGEKPPSMDETAVENGSTHDNK-SEGESAK 911
            +    +     DAD+K G K+   E D +    K   +D    +NGS  D+K  +G  A 
Sbjct: 851  SSKEGEDVSFSDADSKTGKKQSSGEEDSEQSEGKTSDVD-ARDKNGSLDDSKVRKGIEAD 910

Query: 912  SAPNSPFASSAIGSPKESMDSNFGKAAGFDASPR---------DKDAHSDHGGVGSVFSG 971
            S+P +    S  G       ++ GK   +D+            D     DHG   S F  
Sbjct: 911  SSPRTKDTRSENGHDDGESTASAGKTVNYDSHDETDSVSSVNPDNGKDKDHGKYDSGFGF 970

Query: 972  DKSYDEPAWGRFDTNDDV-------------DSVWG--FNAGGSTKTDNDLNRDSY---- 1031
               +D+ +     T   +             DSV     NA   + T   L  DS     
Sbjct: 971  GFGFDDFSIKPIKTGSTISNDFLPPKLSIFADSVPSPPANASDVSPTKPSLFADSVPSTP 1030

Query: 1032 ------------FFDSGELGLNPIRTDPFPAKRSTF--------------------AFDE 1091
                        FFD           + F  K+S F                     FD+
Sbjct: 1031 ATNNASYPGQKSFFDDSVPSTPAYPGNLFAEKKSFFDDSVPSTPAYPGNLFAEKKSYFDD 1090

Query: 1092 SVPSTPLFNSGNSPHNFREGSEPSFDSFLRFDSFGMHDSGIFPP------QDNTFSRFDS 1095
            SVPSTP +++ +          P  D+     S  M DS    P       +N+FSRFDS
Sbjct: 1091 SVPSTPAYSTSDFGGKPFASETPRSDNLFPGRSPFMFDSVPSTPAAHDDFSNNSFSRFDS 1150

BLAST of MC04g0321 vs. TAIR 10
Match: AT4G05520.1 (EPS15 homology domain 2 )

HSP 1 Score: 77.0 bits (188), Expect = 1.0e-13
Identity = 35/81 (43.21%), Postives = 57/81 (70.37%), Query Frame = 0

Query: 13 LFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQIGFLGRAEFYNAL 72
          ++  +F  AD D DGR+SG +A  FF  S L++Q L Q+WA+AD ++ GFLG +EF  A+
Sbjct: 19 IYKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQVWAVADSKRQGFLGLSEFITAM 78

Query: 73 RLVTVAQSKRELTPEIVKAAL 94
          +LV++AQ   E+T +++K ++
Sbjct: 79 KLVSLAQEGHEITSDLLKGSI 99

BLAST of MC04g0321 vs. TAIR 10
Match: AT4G05520.2 (EPS15 homology domain 2 )

HSP 1 Score: 77.0 bits (188), Expect = 1.0e-13
Identity = 35/81 (43.21%), Postives = 57/81 (70.37%), Query Frame = 0

Query: 13 LFDAYFRRADLDRDGRISGAEAVAFFQGSGLAKQVLAQIWALADPRQIGFLGRAEFYNAL 72
          ++  +F  AD D DGR+SG +A  FF  S L++Q L Q+WA+AD ++ GFLG +EF  A+
Sbjct: 19 IYKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQVWAVADSKRQGFLGLSEFITAM 78

Query: 73 RLVTVAQSKRELTPEIVKAAL 94
          +LV++AQ   E+T +++K ++
Sbjct: 79 KLVSLAQEGHEITSDLLKGSI 99