MC04g0302 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0302
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionbrefeldin A-inhibited guanine nucleotide-exchange protein 1-like
LocationMC04: 2396180 .. 2406676 (-)
RNA-Seq ExpressionMC04g0302
SyntenyMC04g0302
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGTGTCGCAAACCCTAGGTGGAACGTCCAGATGCGGCCGCGCGATCGGCCCTTCGCTCGATAAGATTGTCAAGAACGCGGCCTGGCGTAAGCATTCTCACCTCGTTTCTTCCTGTAAATTGGTTCTCGATAAGCTTGATTCTCTAGGGGAGGCTCCTCTTGATCCTGCCTCCGGACTCGCTGGCCTTTCACCGACCGATGCAGAATTCGTGCTCCAACCTCTTCTTTTGGCCCTTGATGCTGCATACGTCAAGGTTGCCGAGCCCGCACTTGAGTGCCTCTTCAAATTGTTCTCCCGCGGTCTTTTTCGTGGCGAGATCGAGCGGCCGGAGAGCGAGGCGAATTCCAGTCCCAGTACCATTGTGTATAAGATCGTTGAGTCTGTTTGCAAGTCTGGAGGTCTCGGCGATGAAGGAATTGAGCTCACTTCTCTCAGAGTATTGCTCTCTGCGGTTCGAAGCCCTTGTGTATTGATCCGCGGGGATTGTCTGGTCAATGTTGTTCGGACTTGTTATAATGTGTATCTCGGTGGCTTAACTGGGACGAATCAGATCTGTGCAAAATCCGTTCTAGCTCAAATTATGGTCGTTGTTTTCAGTAGGGTGGAGGAGGACTCAATGGATGCTCCTATGAGGATAATTTCTGTGAGTGAATTATTGGAATTTACCGATAAGAACTTGAATGAAGGAAATTCTATATATTTTTGCCAGAACTTCGTTAACGAGGTAATGGATGCCAGTGAAGGGATTGCGGAGAAGAAACTATATTCGTTTTCGTCACAATTACAGAATGGCAATGCTTCGCCACTGAAAGTTGACAATAAAGGTGAATCTGATGTAGGAGAAACGGAAGATGGAGCCGAATCAGGTGGTTGTAGTAAGATTAGAGAGGATGGATTCCATTTGTTTAAGAATTTGTGTAAATTGTCAATGAAATTCTCATCACAGGAGCACCCGGATGATCAAATCCTTTTAAGGGGGAAAGTATTGTCTCTGGAGCTGCTAAAGGTTGTTATGGATAATGCAGGTCCAGTTTGGCGTTCTCACGAGAGGCAAGCATTTCATTCTCCATTGATCATTTTAGTGTTACTCACGAACACATCTTTAGAAGTGCCTTTAACTCCTCAGGTCATGCAAAATGTTGACCAAATTAGTTTTCTACCTTTTAATCATGCAATTTTATCTTATTAAACTTGGTATTTTCATTGGGAGAAATTACTTCCGGCAGTCTGAAAATTGTCTCATTTTGTCGGGTACTTTATAGTAAGCTATTGTTGATTCGATAAACTGTGACAGATTCTGTCACTGTTGGTTTCAGGTTTCTCAACGCTATCAAGCAATATCTCTGCTTATCATTATTAAAAAACAGTGCATTATCAGTGATGGCCATCTTCCAGCTTCAATGTTCTATTTTTACGAGCTTGTTAACAAAATTCAGATCGGGATTGAAAGCAGAAATGGGTATCTTCTTTCCCATGCTTGTTCTCCGGGTGCTTGAGAATGTACTTCAGCCTAGTTTCTTGCAGAAAATGACCGTGCTCAATTTATTGGATAAGATATCCCAAGATTCGCAGATTATGGTGGACATTTTTGTCAACTATGATTGCGATGTTGATTCTCCAAATATTTTTGAGAGGTACTGATTTTGCCCATACCTTTTGTTTTACTCTAGTTTAAATTATAGTTTTCCTTTGTTTTCTCAAGGGTTGATATTTTTCTTCCTCTCCTCTGCATGCGAGAATGGGACTGGGGGGGGGGGGGTTTTGTNGTTTGTGGAGGGGGATTTTAGTTTTCATAATGTGATGTTAACCTCATAAGTCACACCCCATTGAAATATTTGCAGGATTGTGAATGGCCTTTTGAAAACTGCTCTTGGACCACCTTCTGGTTCGACCACAGCATTGTCTCCAGCGCAGGATATCACATTTAGGCATGAATCTGTCAAGTGTTTGGTAAGCATAATTAAATCAATGGGAACTTGGATGGACCAACAACTGAAGCTAGATGATACTTCTTTACCCAAGATTCTTGAGAATGATGCTTCACCTGAGAGTCATTCGATTATAAGTGGGGAAGAAACAGCTGCTGTTGACTCTGAGCTCAATACGGATGGAAATTCTGAGTCTTCAGATACTGCTACACTTGAGCAACGAAGAGCTTACAAAATCGAACTCCAGGTTTGAGCATCATCTTATCTTTAGTTAATGCCATTTTATTTATGTAGAGGGTGGGTGACAAGTGGGGCCTCCAATTGTGGTAATGGTAATATTATAATTCAAATTAGTAGGGTTTAAGATGAAGGTTCAAGTTAGGATAAGATGTACAAATTCTATATGGCAATTTCTTGGGAGATTTTTGGAAAGATATGTAACAAAATTTGTGAAGACAACTAGAATGAGGTGGAGTTTTTTTTTGGGGATACGGATAGAGTTTTTGGTTATGGTCTCCTATGGTCATTTATCTCAAGGAAGTTTTGTTAGTGGCTGTTATATTGACAAGTTTGAGAGCCTTTTCGTGGATCTCTTTATTTTGTTTTGTATAAGGTTTATTTAGTCATCTTGTTTGCAACTATCTTTTGCTAGAGACGAAAGTTAAGTTTCTTATATTAAAAAAAACTATCTTTTGCTAGAGATGAAAGTTGTTAATGTTGTTGATGGTTTTATTTATTTATTTGGGTGATAAACCATTTCGTCAAGAAGAGAAAATAAAATAGAGGAAGGGAGATGGGGCATTCTTCCTAGGTTAGATTCATTAGAAGAGATAACAAAAAGACTTCCCAGTTGACATTGGTAAAAAAAAAAAAAACTCTTTTTTTTGAGGTGCAGGTTGATTGTGGTTGGATAGTTCATAGTATAAAATCATTAAAAGTTTCATCTTCTTTTGTGCAGTTGTTTTGAAATATTTTATTATGAATGGTTTGTATTATTTGTTGGCGTCAATAATGATGTACTATAGTTCTTGAACCGCCATGGGTTGATTTAGTGGTTTATAAAGCCAATGTGAATAAAAAAACTCAGAAGGAATGAGTTCAAGTCAATGTGGCCTGGCCACCTACGCAGAATTTAATATTATTCGACTTACCTTAGCAACCAAATATTATAGGGTCAGATAGTTGTCCTAAGAGAATGATCAAGATGCATGCAAATCTGGCTTGAACAATCACATATATCAATGAAAAAAAGATGTACTATATTTCTTGAGAATCATTGAATTCTGTGTTTCTTTTTTCTTTTTTATTTGCCTTTTGGTCTAGGACACCTCATTCTTGGATATTTTTTTGAACTCAAACATTCTGTGTTTTTGCTGGTAAAAAAAAAAAAGGAAAACATTAGCTGTTCCTTATCAAAAAAGAAAAAAAAATTCATGTATGTGCACATAAGTGGACTTTATTGCTCTAATCGGATATTTGGAACTCATTAAAAGCCTGAAGAGAAAAATATATGGTTCACTGTGATGAAGATCCTTTTTAACTGTTCCTTTTTCAGATTTGATTATTTTTTAGTTTCAGATTTTTAGTCATTTGGGCCTATTTAAAGGCTTGTTCTTCGAACTGATTTCATAATCTCTTGGGAATAAGAATAACAAATTTGAGTATTACAATTTACATTACACTATATAAAGTTTAAAGGGTTAGAAAATTTTGCTCGTTTGTTGATTGCATGTTGGCCAATTAGAAAGCACTTTGCTTCTTGTAATTTGCCTTTCCTCCTTGGTTGTTCCTTACTAATTGTTCCTGTTAATCGGATTGGCAATTTCCTCAAATCTTGCTTGGTTAGTGTATATTCATCATATAATCTATTATTTTCAACACATTTCCATCTTAGAGGTAATTAGCCTGAAGCACATTGTTCTAATAACTTTCCTGATTTATGTTCAGAAAGGTATTTCGCTGTTCAATAGGAAACCTTCCAGGGGTATTGAGTTTCTGATGAGCACAAAAAAAGTTGGTGGTTCCCCTGAAGAGGTGGCTTCTTTTCTCAAGAACACTAGCGGCCTGAATGAAACGGTCATTGGGGATTATTTGGGTGAAAGGGAGGAATTCCCCTTGAAAGTTATGCATGCTTATGTAGATTCATTTAATTTTAAAGCGATGGATTTTGGTGAAGCAATAAGATTTTTCCTACGAGGCTTCAGGTTACCAGGAGAGGCACAGAAGATTGACCGCATTATGGAGAAGTTTGCTGAGCGGTACTGTAAATGTAACCCTGGTTCATTTGCAAGGGCAGATACTGCTTATGTTCTTGCTTATTCTGTAATAATGCTGAACACAGATGCTCATAATAACATGGTGAAAGATAAGGTTTGCCTCTGACTTATGCACTCGATCGTATATTGCATGTTTAACAGCCTGCATGTAAAGCTAAGGATGTTATTTAGGTTACCATCTATGACTCTGATCCTGTTAACTATAGTCTATATGTCTTTTTCGATTAGTTCCTTTTGTGGAGCTTTCATATCTAATCTCCTTGGCTTGGCCAAAAGGTCCAAAGATTGGATGGTGAAGATGTTATCGATTTTGAACCAGTGTCCGTTATTTACTCAGAAGGGGTAGAAAAAATTTTCGAAAAAAAAAACAAATGCAATATCTACCGTATATAAACACATTGGGATTATATTTGGCTACTATGTCCATTGTCCATGGAAGCAAAACATTAAACGGGCCAGAACAGTCCTCAAGTACCTAAAAGAGCCAGTCATTCTGACCTAATTAATCATGTGCATTTCCCCTTGGGCCTTTTCTATATGCTTTCTATTTATACTAATATGGTTCTGATCCGAAAAAAATTGTAGCCTGGGGATCTGTCTCTTCATCATGAGAACAGTACTGCCCCATCTAATAGGTTCATTTTAATTGCAAAATTGTAAAGTTTTTCATCGTTTTATGTGGGAGATGTGAACGGAGGCTAATGCATGCTTCGTGAATGATTTCACAGAAGTAATATTGTCAGACTCATTGCATGTTTAAGATTCACAGAACTTGATTTCTGGAGTTTTTTGCCTTCTGGTGGCCGGTGGATGTATGCAACTATTTCTGAACATATAGCATATACTGCTTCTTTTCATTGTTAAGTTTTGTTTACACATTGATAAAAGAAGTTTGAGTTGTTTTTTGAGTTTTACACAAATCATAAAACGTATTTAGACGTTTTTCTGACTTCTGTTATGCATTGGTTGGATATTCAGATGACAAAAGCCGATTTTATCCGGAATAATCGAGGAATTGATGACGGCAAGGATTTACCTGATGAGTATCTTGGTGCTCTTTATGATCAGATTATTAGGAATGAAATTAAGATGAATTCTGATTCTTCAGCATCACAAAGTAAGCAGGCAACTAGCATCAACAAACTTTTGGGTTTGGATGGTATACTCAATCTAGTTTCTTGGAAGCAGACAGAAGAAAAAGCCGTTGGTGCAAATGGGCTTCTTATAAGGCACATACAGGAGCAGTTTAAGGCGAAGTCTGGAAAGTCTGAGTAAGCTATGAAATGGAATTTTATCTGTTATTTCTGTTCTGCTATTGAGAATATGAGTTTTTTTTGGTGGCAATAGTTTTCCCTTAGTAACTTGAATGTGCAAAGCTCTTCTGGTTCATTATTGGCTCTATCTGTTGTATTAGATAGAGTGATATGGCTTTCCTTTTTGCTCATTTTCTGTTAGTTTATTGTGGATTTTCATCGAATTGATCTGCCATGTATATGTTGTAAAATATATATTTGTAATTTGTCAATAGAATATTTTATTTTTTCGCCTAAAAGATTAGAACTTACTCTTATTGAAAAAGGAACTTATTTTCATCTGCATTTGCAGATCTGTTTATCATGCTGTGACTGATGTAACAATATTGAGGTTTATGGTGGAAGTATGCTGGGGTCCTATGTTGGCGGCATTTAGCGTGACTCTTGACCAAAGCGATGATAAGCTTGCTACTTCTCAATGCTTGCTAGGCTTTCGGCATGCTGTGCATGTTACAGCCATGATGGGCTTGCAGACTCAAAGAGATGCTTTTGTGACATCCATGGCTAAGTTTACCTATCTTCATTGTGCTGCAGATATGAAACAAAAAAATGTTGACGCTGTGAAGGTATGCATATTCTTATCATTGAGACAGCATGTGTTTTTTTTTCCTTGAAAAAAATTTCTCACGAAGGAAGCTTAAACTGTAGGTACATTTTTCTGTATTACCCGATGAAATTAAGAAAAGATCTAATACATGGTGACTTGTATAGGCTACAAGAACTAAAAAGGATGCTCATTTTTCCACCCCTTAGAAGCACATGCTTTATCTCAAAACACTTGAACCTTTCAAATTAGGTCTAATGGGCAAGCACTGTGTTAAGGAAAAAGCCTGTTCTTAGAAGGGAAGACAATAATTTATGGATGTGTTTAGTTTCTCGGTTCATATTGAATTATACATGAAGAACTAACTACATACTGTGTGGTCTTTTAAATTCTGTTGCATGGTCAACTCTTGCAAGTCGACGAGTTTCTTTCTTTTTTTTTTTTTTGAAAATAAATGGTCCTTTTTCCTTCTCTGAATGTGCACGTGCAGGCAATAATATCAATTGCCATAGAGGATGGTAATTTCCTACAAGAGGCATGGGAACACATATTTACATGTCTCTCTAGAATTGAGAATTTACAATTGTTGGGAGAGGGTGCACCACCCGATGCATCGTTTTTGACTTCATCAATTATTGAAACTGACGAGAAAACAATGAAGTCAGTGGGTTTATCATCTCTGAAGAAAAAAGGAGGTCTCCAAAATCCAGCTGTTATGGCTGTTGTTCGAGGAGGTTCATACGATAGCACCTCTCTTGGAGTGAATTCTCCAGGACCAGTAACTCCGGAACAAATTAACCACCTTATTTCAAACTTGAATTTGCTTGATCAAATAGGCAATTTTGAGTTGAACCACGTATTTGCTCATAGTCAAAGCTTGAACAGTGAAGCAATAGTAGCATTTGTGAAGGCACTTTGCAAAGTTTCTATTGCAGAGTTGCAGTCTCCAACAGACCCACGTGTGTTCAGCCTCACTAAATTAGTTGAAGTGGCGTAAGATCTTTGTCTTTACTATGAGTTACTGCATCTGCATAATATTTAATGTCCTAAAGTAATGAAATATTTAGGTTTAAAGGACTATTTATATAATAGTATTTATTGTATCTAGGGTTCCATGACACTCCAGTTAGGTAATGCTTCAATTTTTCAAAATGCGTCCCCTCTACAGTCTCTCTGAATTACCCATCATAATAAGCCTATTGGACAACATCTCTATGATTTATGCGAACATTTAGAAATGGAATGGGAAAATCATCACAAAAAATTGTTTCTTTTGCAGGCATTACAACATGAACCGCATCAGATTGGTGTGGTCTCGCATGTGGAATGTTCTCTCTGATTTCTTTGTCTCAGTTGGCTTATCAGAAAATCTGTCAGTCGCAATATTTGTGATGGACTCACTGAGGCAGCTCGCAATGAAATTTTTGGAGCGTGAGGAGCTTGCAAATTATAACTTCCAGAACGAATTTCTGAGACCCTTCGTAATTGTTATGCAGAAAAGCAGCTCCACAGAAATCAGGGAATTAATAGTTCGGTGCATTTCACAGATGGTTCTCAGTCGCGTCAATAATGTGAAATCTGGATGGAAAAGTGTCTTTTTGGTACGTTCCAAACATTAGTAATAAGATTGGGTGCTACAATACTAACATATTGCTAGAACAGAAAGTTTAAACAATTGGGTTGGGTAGATCTATTCACGTCTTTAAATTTCAGTGCCCCTTTTGTGTCACTGCGAACTTTACCTTTTACTCACATATGGACTGTTAATTCAAGTAGGGAAGGGATGTCAATGCAAAAAGTTGACTAAAATTCCAAATTACACAATTTTAAAATTGAAGCTATTAGGTTCGGATTAATTTGATCCTGTGTTATTTCTTTAACATATTATGATGCATTCTTCCAGACAAGTAATATTAAATAGATTAAAAAAAAGATCTTAGGAGTCCTCCCTCCCAATTACGATTTTTTACAATGAGCACAATTGAGTCGTCATCTTATATGATCACCTTACAACTCACCTATTGTCTAAGGTATTCACTGCTGCTGCTGCTGATGAGCGGAAGAATATTGTCTTATTGGCCTTTGAAACCATGGAAAAAATAGTTCGAGAGTACTTTCCTTATATAACTGAGACTGAAACTACAACATTTACCGATTGCGTCCGATGTCTCATTACCTTCACGAATAGCAGATTTAATAGTGATGTCAGTCTCAATGCCATTGCGTTTCTTCGGTTTTGTGCTGTTAAACTTGCAGAAGGGGGACTTGTTTGTTATGAGATGGTTGTAGATGATGATTCATCCAAAGCTCCAGATGCACCTACATCCACAGATAAAGATGATTATGCTTCCTATTGGGTTCCTTTGCTTGCAGGTAAAACTGAATTTTTTGCTCGATTGGGTGCAATTGTTTTCATTATACGTACTGGGGAAGTCACCTTTTTTTATGCATTTTTCTAGTATCTTTTGTTATGGGGATAAATTTTTTTTGTGATTGTTATTTGCTATGCTATATCAGCTGTTAGTGTTTTCATCCCTTCCACGGATGATTGTGTCCTTGCATCAAAATCAATGAGCAAATTTACATTTTTCCTCTTCACACTGATTGAATAGCAGAAACATACATAGCTGTCAGTTTTGTTGCTTTGAGATGTATATGTGAGACTTTCATGTTTATATCCTGTGAAGACATAATTTGGGTTTGGAATCTACATAATATTTTTTTGTTATCCTTTCAGGGCTATCAAAGTTAACATCTGATCCTAGATCACCAATCAGAAAAAGTTCCTTGGAAGTACTTTTCAATATCCTAAAGGACCACGGTCATCTTTTCTCGCGTCAATTTTGGGTTGGTGTTATCAATACTGTAGTTTTCCCCATATTCAGTTCTCTTTATGGCAAGAAAGAAGTGGACATCAATGAAAGTGATGAACATCCAGAAGGAAGTACATGGGATTCTGATACCTGTGTTGTTGCTGCAGAGTGTTTAGTAGATCTGTTCATTAGTTTTTTCAATGTAATAAGGTCTCAACTACCGGGCGTGGTTGCGATTCTGACAGGATTCATTAGGAGTCCCATCCATGGTCCTGCAAGCACGGGCGTTTCTGCGCTAATGCGTTTGGCAGGCGAATTGGGCAGCAGGCTTTCAGGAAGTGAGTGGAGAGAGATTTTTCTTGCTTTGAAGGAAGCGGCAACATCAACCGTGCCTGATTTTATGAAGGTCCTGAAAACCATGGATGATGTCAATGTTCCCGGGATTTCTCAGTCTTCTTATGATGTCGATGTGGCTTCTGATCAAGGTTTGTCAACTGATGGTCTCGATGACGATGATCTGCAAACGGCCTCATACATAGTTTCAAGAATGAAGAGTCATATCGGCATGCAACTCCTCATCATACAGGTTAGCTTCCTTCTCATGTTTCAAAAAGCCTTTTCTTTTACAGAATATAACATTTTCCCCTTATGTCTTTCATCAGGTCATTACTGATCTGTACAAGAATCATGCCCAACCTTTCTCGGAAGACAACATTTCCATCATTCTTGAGATCTTTTCATCCATTTCCACACATGCTCAGAAACTGAATTCTGAGATAATCCTTCAGAAGAAGCTTCAGAAAGCTTGCTCCATCCTTGAGATATCCGACCCGCCAGTAGTTCACTTCGAAAACGAGTCATATCAAAGCTACCTCAATTTTCTCCAGAATATGCTCATGGATACTTCGTCCCTGACCAATCCAACACTTGTAGAATCAGAACTTGTCGCAGTATGTGAACAAATTTTGCGCATTTACTTGAAGTGTACCAGGACGCAGGCCGAGCGGAAAGAAACCAACAACCCTGTGCTACATTGGATTCTTCCATTGGGATCGGCCAAGAAGGAGGAACTGGCTGCCCGGACGTCTCTCGTTGTCTCGGCATTGCGGGTTCTATCTGGGCTCCAAAGGGATCTATTCAAAAGGTATGTTTCACAACTTTTTCCATTGTTGGTAGATCTTGTAAGGAGTGAGCATAGCTCTGGAGAAGTTCAGCTTGTTTTGAGCAGCATATTTCAATCATGTATAGGCCCAATAATTATTATGCAATAATAAGATATCCAAAAAATATATATAATATATATATATTCTTTGAGGTATACTTAGCATGCCATTGTAAATCAATTTTTAACTAGGTGGCTTGAGGGGTGGAGCCAGCCACTTCGTTTTGTAGAATATGCCAAGTCCCAAAATTTTTGCCTAGTGAAAGAGGATGTATTTGCCTAGTATTGATAATATATATATATATAAATATGAGAGGAAATTTTTTTGATTTATTGCATCCATATTTTGGGTTTTTAATTCCATATGGTTATCATTTTTAGGTTTCTTTTTCCTTAAATAAACTCCATTGCTGAGGATTTGAGCCATTTTTTCGATATTTTTTCTTGGAAATTCTC

mRNA sequence

ATGTCGGTGTCGCAAACCCTAGGTGGAACGTCCAGATGCGGCCGCGCGATCGGCCCTTCGCTCGATAAGATTGTCAAGAACGCGGCCTGGCGTAAGCATTCTCACCTCGTTTCTTCCTGTAAATTGGTTCTCGATAAGCTTGATTCTCTAGGGGAGGCTCCTCTTGATCCTGCCTCCGGACTCGCTGGCCTTTCACCGACCGATGCAGAATTCGTGCTCCAACCTCTTCTTTTGGCCCTTGATGCTGCATACGTCAAGGTTGCCGAGCCCGCACTTGAGTGCCTCTTCAAATTGTTCTCCCGCGGTCTTTTTCGTGGCGAGATCGAGCGGCCGGAGAGCGAGGCGAATTCCAGTCCCAGTACCATTGTGTATAAGATCGTTGAGTCTGTTTGCAAGTCTGGAGGTCTCGGCGATGAAGGAATTGAGCTCACTTCTCTCAGAGTATTGCTCTCTGCGGTTCGAAGCCCTTGTGTATTGATCCGCGGGGATTGTCTGGTCAATGTTGTTCGGACTTGTTATAATGTGTATCTCGGTGGCTTAACTGGGACGAATCAGATCTGTGCAAAATCCGTTCTAGCTCAAATTATGGTCGTTGTTTTCAGTAGGGTGGAGGAGGACTCAATGGATGCTCCTATGAGGATAATTTCTGTGAGTGAATTATTGGAATTTACCGATAAGAACTTGAATGAAGGAAATTCTATATATTTTTGCCAGAACTTCGTTAACGAGGTAATGGATGCCAGTGAAGGGATTGCGGAGAAGAAACTATATTCGTTTTCGTCACAATTACAGAATGGCAATGCTTCGCCACTGAAAGTTGACAATAAAGGTGAATCTGATGTAGGAGAAACGGAAGATGGAGCCGAATCAGGTGGTTGTAGTAAGATTAGAGAGGATGGATTCCATTTGTTTAAGAATTTGTGTAAATTGTCAATGAAATTCTCATCACAGGAGCACCCGGATGATCAAATCCTTTTAAGGGGGAAAGTATTGTCTCTGGAGCTGCTAAAGGTTGTTATGGATAATGCAGGTCCAGTTTGGCATTCTGTCACTGTTGGTTTCAGGTTTCTCAACGCTATCAAGCAATATCTCTGCTTATCATTATTAAAAAACAGTGCATTATCAGTGATGGCCATCTTCCAGCTTCAATGTTCTATTTTTACGAGCTTGTTAACAAAATTCAGATCGGGATTGAAAGCAGAAATGGGTATCTTCTTTCCCATGCTTGTTCTCCGGGTGCTTGAGAATGTACTTCAGCCTAGTTTCTTGCAGAAAATGACCGTGCTCAATTTATTGGATAAGATATCCCAAGATTCGCAGATTATGGTGGACATTTTTGTCAACTATGATTGCGATGTTGATTCTCCAAATATTTTTGAGAGGATTGTGAATGGCCTTTTGAAAACTGCTCTTGGACCACCTTCTGGTTCGACCACAGCATTGTCTCCAGCGCAGGATATCACATTTAGGCATGAATCTGTCAAGTGTTTGGTAAGCATAATTAAATCAATGGGAACTTGGATGGACCAACAACTGAAGCTAGATGATACTTCTTTACCCAAGATTCTTGAGAATGATGCTTCACCTGAGAGTCATTCGATTATAAGTGGGGAAGAAACAGCTGCTGTTGACTCTGAGCTCAATACGGATGGAAATTCTGAGTCTTCAGATACTGCTACACTTGAGCAACGAAGAGCTTACAAAATCGAACTCCAGAAAGGTATTTCGCTGTTCAATAGGAAACCTTCCAGGGGTATTGAGTTTCTGATGAGCACAAAAAAAGTTGGTGGTTCCCCTGAAGAGGTGGCTTCTTTTCTCAAGAACACTAGCGGCCTGAATGAAACGGTCATTGGGGATTATTTGGGTGAAAGGGAGGAATTCCCCTTGAAAGTTATGCATGCTTATGTAGATTCATTTAATTTTAAAGCGATGGATTTTGGTGAAGCAATAAGATTTTTCCTACGAGGCTTCAGGTTACCAGGAGAGGCACAGAAGATTGACCGCATTATGGAGAAGTTTGCTGAGCGGTACTGTAAATGTAACCCTGGTTCATTTGCAAGGGCAGATACTGCTTATGTTCTTGCTTATTCTGTAATAATGCTGAACACAGATGCTCATAATAACATGGTGAAAGATAAGATGACAAAAGCCGATTTTATCCGGAATAATCGAGGAATTGATGACGGCAAGGATTTACCTGATGAGTATCTTGGTGCTCTTTATGATCAGATTATTAGGAATGAAATTAAGATGAATTCTGATTCTTCAGCATCACAAAGTAAGCAGGCAACTAGCATCAACAAACTTTTGGGTTTGGATGGTATACTCAATCTAGTTTCTTGGAAGCAGACAGAAGAAAAAGCCGTTGGTGCAAATGGGCTTCTTATAAGGCACATACAGGAGCAGTTTAAGGCGAAGTCTGGAAAGTCTGAATCTGTTTATCATGCTGTGACTGATGTAACAATATTGAGGTTTATGGTGGAAGTATGCTGGGGTCCTATGTTGGCGGCATTTAGCGTGACTCTTGACCAAAGCGATGATAAGCTTGCTACTTCTCAATGCTTGCTAGGCTTTCGGCATGCTGTGCATGTTACAGCCATGATGGGCTTGCAGACTCAAAGAGATGCTTTTGTGACATCCATGGCTAAGTTTACCTATCTTCATTGTGCTGCAGATATGAAACAAAAAAATGTTGACGCTGTGAAGGCAATAATATCAATTGCCATAGAGGATGGTAATTTCCTACAAGAGGCATGGGAACACATATTTACATGTCTCTCTAGAATTGAGAATTTACAATTGTTGGGAGAGGGTGCACCACCCGATGCATCGTTTTTGACTTCATCAATTATTGAAACTGACGAGAAAACAATGAAGTCAGTGGGTTTATCATCTCTGAAGAAAAAAGGAGGTCTCCAAAATCCAGCTGTTATGGCTGTTGTTCGAGGAGGTTCATACGATAGCACCTCTCTTGGAGTGAATTCTCCAGGACCAGTAACTCCGGAACAAATTAACCACCTTATTTCAAACTTGAATTTGCTTGATCAAATAGGCAATTTTGAGTTGAACCACGTATTTGCTCATAGTCAAAGCTTGAACAGTGAAGCAATAGTAGCATTTGTGAAGGCACTTTGCAAAGTTTCTATTGCAGAGTTGCAGTCTCCAACAGACCCACGTGTGTTCAGCCTCACTAAATTAGTTGAAGTGGCGCATTACAACATGAACCGCATCAGATTGGTGTGGTCTCGCATGTGGAATGTTCTCTCTGATTTCTTTGTCTCAGTTGGCTTATCAGAAAATCTGTCAGTCGCAATATTTGTGATGGACTCACTGAGGCAGCTCGCAATGAAATTTTTGGAGCGTGAGGAGCTTGCAAATTATAACTTCCAGAACGAATTTCTGAGACCCTTCGTAATTGTTATGCAGAAAAGCAGCTCCACAGAAATCAGGGAATTAATAGTTCGGTGCATTTCACAGATGGTTCTCAGTCGCGTCAATAATGTGAAATCTGGATGGAAAAGTGTCTTTTTGGTATTCACTGCTGCTGCTGCTGATGAGCGGAAGAATATTGTCTTATTGGCCTTTGAAACCATGGAAAAAATAGTTCGAGAGTACTTTCCTTATATAACTGAGACTGAAACTACAACATTTACCGATTGCGTCCGATGTCTCATTACCTTCACGAATAGCAGATTTAATAGTGATGTCAGTCTCAATGCCATTGCGTTTCTTCGGTTTTGTGCTGTTAAACTTGCAGAAGGGGGACTTGTTTGTTATGAGATGGTTGTAGATGATGATTCATCCAAAGCTCCAGATGCACCTACATCCACAGATAAAGATGATTATGCTTCCTATTGGGTTCCTTTGCTTGCAGGGCTATCAAAGTTAACATCTGATCCTAGATCACCAATCAGAAAAAGTTCCTTGGAAGTACTTTTCAATATCCTAAAGGACCACGGTCATCTTTTCTCGCGTCAATTTTGGGTTGGTGTTATCAATACTGTAGTTTTCCCCATATTCAGTTCTCTTTATGGCAAGAAAGAAGTGGACATCAATGAAAGTGATGAACATCCAGAAGGAAGTACATGGGATTCTGATACCTGTGTTGTTGCTGCAGAGTGTTTAGTAGATCTGTTCATTAGTTTTTTCAATGTAATAAGGTCTCAACTACCGGGCGTGGTTGCGATTCTGACAGGATTCATTAGGAGTCCCATCCATGGTCCTGCAAGCACGGGCGTTTCTGCGCTAATGCGTTTGGCAGGCGAATTGGGCAGCAGGCTTTCAGGAAGTGAGTGGAGAGAGATTTTTCTTGCTTTGAAGGAAGCGGCAACATCAACCGTGCCTGATTTTATGAAGGTCCTGAAAACCATGGATGATGTCAATGTTCCCGGGATTTCTCAGTCTTCTTATGATGTCGATGTGGCTTCTGATCAAGGTTTGTCAACTGATGGTCTCGATGACGATGATCTGCAAACGGCCTCATACATAGTTTCAAGAATGAAGAGTCATATCGGCATGCAACTCCTCATCATACAGGTCATTACTGATCTGTACAAGAATCATGCCCAACCTTTCTCGGAAGACAACATTTCCATCATTCTTGAGATCTTTTCATCCATTTCCACACATGCTCAGAAACTGAATTCTGAGATAATCCTTCAGAAGAAGCTTCAGAAAGCTTGCTCCATCCTTGAGATATCCGACCCGCCAGTAGTTCACTTCGAAAACGAGTCATATCAAAGCTACCTCAATTTTCTCCAGAATATGCTCATGGATACTTCGTCCCTGACCAATCCAACACTTGTAGAATCAGAACTTGTCGCAGTATGTGAACAAATTTTGCGCATTTACTTGAAGTGTACCAGGACGCAGGCCGAGCGGAAAGAAACCAACAACCCTGTGCTACATTGGATTCTTCCATTGGGATCGGCCAAGAAGGAGGAACTGGCTGCCCGGACGTCTCTCGTTGTCTCGGCATTGCGGGTTCTATCTGGGCTCCAAAGGGATCTATTCAAAAGGTATGTTTCACAACTTTTTCCATTGTTGGTAGATCTTGTAAGGAGTGAGCATAGCTCTGGAGAAGTTCAGCTTGTTTTGAGCAGCATATTTCAATCATGTATAGGCCCAATAATTATTATGCAATAATAAGATATCCAAAAAATATATATAATATATATATATTCTTTGAGGTATACTTAGCATGCCATTGTAAATCAATTTTTAACTAGGTGGCTTGAGGGGTGGAGCCAGCCACTTCGTTTTGTAGAATATGCCAAGTCCCAAAATTTTTGCCTAGTGAAAGAGGATGTATTTGCCTAGTATTGATAATATATATATATATAAATATGAGAGGAAATTTTTTTGATTTATTGCATCCATATTTTGGGTTTTTAATTCCATATGGTTATCATTTTTAGGTTTCTTTTTCCTTAAATAAACTCCATTGCTGAGGATTTGAGCCATTTTTTCGATATTTTTTCTTGGAAATTCTC

Coding sequence (CDS)

ATGTCGGTGTCGCAAACCCTAGGTGGAACGTCCAGATGCGGCCGCGCGATCGGCCCTTCGCTCGATAAGATTGTCAAGAACGCGGCCTGGCGTAAGCATTCTCACCTCGTTTCTTCCTGTAAATTGGTTCTCGATAAGCTTGATTCTCTAGGGGAGGCTCCTCTTGATCCTGCCTCCGGACTCGCTGGCCTTTCACCGACCGATGCAGAATTCGTGCTCCAACCTCTTCTTTTGGCCCTTGATGCTGCATACGTCAAGGTTGCCGAGCCCGCACTTGAGTGCCTCTTCAAATTGTTCTCCCGCGGTCTTTTTCGTGGCGAGATCGAGCGGCCGGAGAGCGAGGCGAATTCCAGTCCCAGTACCATTGTGTATAAGATCGTTGAGTCTGTTTGCAAGTCTGGAGGTCTCGGCGATGAAGGAATTGAGCTCACTTCTCTCAGAGTATTGCTCTCTGCGGTTCGAAGCCCTTGTGTATTGATCCGCGGGGATTGTCTGGTCAATGTTGTTCGGACTTGTTATAATGTGTATCTCGGTGGCTTAACTGGGACGAATCAGATCTGTGCAAAATCCGTTCTAGCTCAAATTATGGTCGTTGTTTTCAGTAGGGTGGAGGAGGACTCAATGGATGCTCCTATGAGGATAATTTCTGTGAGTGAATTATTGGAATTTACCGATAAGAACTTGAATGAAGGAAATTCTATATATTTTTGCCAGAACTTCGTTAACGAGGTAATGGATGCCAGTGAAGGGATTGCGGAGAAGAAACTATATTCGTTTTCGTCACAATTACAGAATGGCAATGCTTCGCCACTGAAAGTTGACAATAAAGGTGAATCTGATGTAGGAGAAACGGAAGATGGAGCCGAATCAGGTGGTTGTAGTAAGATTAGAGAGGATGGATTCCATTTGTTTAAGAATTTGTGTAAATTGTCAATGAAATTCTCATCACAGGAGCACCCGGATGATCAAATCCTTTTAAGGGGGAAAGTATTGTCTCTGGAGCTGCTAAAGGTTGTTATGGATAATGCAGGTCCAGTTTGGCATTCTGTCACTGTTGGTTTCAGGTTTCTCAACGCTATCAAGCAATATCTCTGCTTATCATTATTAAAAAACAGTGCATTATCAGTGATGGCCATCTTCCAGCTTCAATGTTCTATTTTTACGAGCTTGTTAACAAAATTCAGATCGGGATTGAAAGCAGAAATGGGTATCTTCTTTCCCATGCTTGTTCTCCGGGTGCTTGAGAATGTACTTCAGCCTAGTTTCTTGCAGAAAATGACCGTGCTCAATTTATTGGATAAGATATCCCAAGATTCGCAGATTATGGTGGACATTTTTGTCAACTATGATTGCGATGTTGATTCTCCAAATATTTTTGAGAGGATTGTGAATGGCCTTTTGAAAACTGCTCTTGGACCACCTTCTGGTTCGACCACAGCATTGTCTCCAGCGCAGGATATCACATTTAGGCATGAATCTGTCAAGTGTTTGGTAAGCATAATTAAATCAATGGGAACTTGGATGGACCAACAACTGAAGCTAGATGATACTTCTTTACCCAAGATTCTTGAGAATGATGCTTCACCTGAGAGTCATTCGATTATAAGTGGGGAAGAAACAGCTGCTGTTGACTCTGAGCTCAATACGGATGGAAATTCTGAGTCTTCAGATACTGCTACACTTGAGCAACGAAGAGCTTACAAAATCGAACTCCAGAAAGGTATTTCGCTGTTCAATAGGAAACCTTCCAGGGGTATTGAGTTTCTGATGAGCACAAAAAAAGTTGGTGGTTCCCCTGAAGAGGTGGCTTCTTTTCTCAAGAACACTAGCGGCCTGAATGAAACGGTCATTGGGGATTATTTGGGTGAAAGGGAGGAATTCCCCTTGAAAGTTATGCATGCTTATGTAGATTCATTTAATTTTAAAGCGATGGATTTTGGTGAAGCAATAAGATTTTTCCTACGAGGCTTCAGGTTACCAGGAGAGGCACAGAAGATTGACCGCATTATGGAGAAGTTTGCTGAGCGGTACTGTAAATGTAACCCTGGTTCATTTGCAAGGGCAGATACTGCTTATGTTCTTGCTTATTCTGTAATAATGCTGAACACAGATGCTCATAATAACATGGTGAAAGATAAGATGACAAAAGCCGATTTTATCCGGAATAATCGAGGAATTGATGACGGCAAGGATTTACCTGATGAGTATCTTGGTGCTCTTTATGATCAGATTATTAGGAATGAAATTAAGATGAATTCTGATTCTTCAGCATCACAAAGTAAGCAGGCAACTAGCATCAACAAACTTTTGGGTTTGGATGGTATACTCAATCTAGTTTCTTGGAAGCAGACAGAAGAAAAAGCCGTTGGTGCAAATGGGCTTCTTATAAGGCACATACAGGAGCAGTTTAAGGCGAAGTCTGGAAAGTCTGAATCTGTTTATCATGCTGTGACTGATGTAACAATATTGAGGTTTATGGTGGAAGTATGCTGGGGTCCTATGTTGGCGGCATTTAGCGTGACTCTTGACCAAAGCGATGATAAGCTTGCTACTTCTCAATGCTTGCTAGGCTTTCGGCATGCTGTGCATGTTACAGCCATGATGGGCTTGCAGACTCAAAGAGATGCTTTTGTGACATCCATGGCTAAGTTTACCTATCTTCATTGTGCTGCAGATATGAAACAAAAAAATGTTGACGCTGTGAAGGCAATAATATCAATTGCCATAGAGGATGGTAATTTCCTACAAGAGGCATGGGAACACATATTTACATGTCTCTCTAGAATTGAGAATTTACAATTGTTGGGAGAGGGTGCACCACCCGATGCATCGTTTTTGACTTCATCAATTATTGAAACTGACGAGAAAACAATGAAGTCAGTGGGTTTATCATCTCTGAAGAAAAAAGGAGGTCTCCAAAATCCAGCTGTTATGGCTGTTGTTCGAGGAGGTTCATACGATAGCACCTCTCTTGGAGTGAATTCTCCAGGACCAGTAACTCCGGAACAAATTAACCACCTTATTTCAAACTTGAATTTGCTTGATCAAATAGGCAATTTTGAGTTGAACCACGTATTTGCTCATAGTCAAAGCTTGAACAGTGAAGCAATAGTAGCATTTGTGAAGGCACTTTGCAAAGTTTCTATTGCAGAGTTGCAGTCTCCAACAGACCCACGTGTGTTCAGCCTCACTAAATTAGTTGAAGTGGCGCATTACAACATGAACCGCATCAGATTGGTGTGGTCTCGCATGTGGAATGTTCTCTCTGATTTCTTTGTCTCAGTTGGCTTATCAGAAAATCTGTCAGTCGCAATATTTGTGATGGACTCACTGAGGCAGCTCGCAATGAAATTTTTGGAGCGTGAGGAGCTTGCAAATTATAACTTCCAGAACGAATTTCTGAGACCCTTCGTAATTGTTATGCAGAAAAGCAGCTCCACAGAAATCAGGGAATTAATAGTTCGGTGCATTTCACAGATGGTTCTCAGTCGCGTCAATAATGTGAAATCTGGATGGAAAAGTGTCTTTTTGGTATTCACTGCTGCTGCTGCTGATGAGCGGAAGAATATTGTCTTATTGGCCTTTGAAACCATGGAAAAAATAGTTCGAGAGTACTTTCCTTATATAACTGAGACTGAAACTACAACATTTACCGATTGCGTCCGATGTCTCATTACCTTCACGAATAGCAGATTTAATAGTGATGTCAGTCTCAATGCCATTGCGTTTCTTCGGTTTTGTGCTGTTAAACTTGCAGAAGGGGGACTTGTTTGTTATGAGATGGTTGTAGATGATGATTCATCCAAAGCTCCAGATGCACCTACATCCACAGATAAAGATGATTATGCTTCCTATTGGGTTCCTTTGCTTGCAGGGCTATCAAAGTTAACATCTGATCCTAGATCACCAATCAGAAAAAGTTCCTTGGAAGTACTTTTCAATATCCTAAAGGACCACGGTCATCTTTTCTCGCGTCAATTTTGGGTTGGTGTTATCAATACTGTAGTTTTCCCCATATTCAGTTCTCTTTATGGCAAGAAAGAAGTGGACATCAATGAAAGTGATGAACATCCAGAAGGAAGTACATGGGATTCTGATACCTGTGTTGTTGCTGCAGAGTGTTTAGTAGATCTGTTCATTAGTTTTTTCAATGTAATAAGGTCTCAACTACCGGGCGTGGTTGCGATTCTGACAGGATTCATTAGGAGTCCCATCCATGGTCCTGCAAGCACGGGCGTTTCTGCGCTAATGCGTTTGGCAGGCGAATTGGGCAGCAGGCTTTCAGGAAGTGAGTGGAGAGAGATTTTTCTTGCTTTGAAGGAAGCGGCAACATCAACCGTGCCTGATTTTATGAAGGTCCTGAAAACCATGGATGATGTCAATGTTCCCGGGATTTCTCAGTCTTCTTATGATGTCGATGTGGCTTCTGATCAAGGTTTGTCAACTGATGGTCTCGATGACGATGATCTGCAAACGGCCTCATACATAGTTTCAAGAATGAAGAGTCATATCGGCATGCAACTCCTCATCATACAGGTCATTACTGATCTGTACAAGAATCATGCCCAACCTTTCTCGGAAGACAACATTTCCATCATTCTTGAGATCTTTTCATCCATTTCCACACATGCTCAGAAACTGAATTCTGAGATAATCCTTCAGAAGAAGCTTCAGAAAGCTTGCTCCATCCTTGAGATATCCGACCCGCCAGTAGTTCACTTCGAAAACGAGTCATATCAAAGCTACCTCAATTTTCTCCAGAATATGCTCATGGATACTTCGTCCCTGACCAATCCAACACTTGTAGAATCAGAACTTGTCGCAGTATGTGAACAAATTTTGCGCATTTACTTGAAGTGTACCAGGACGCAGGCCGAGCGGAAAGAAACCAACAACCCTGTGCTACATTGGATTCTTCCATTGGGATCGGCCAAGAAGGAGGAACTGGCTGCCCGGACGTCTCTCGTTGTCTCGGCATTGCGGGTTCTATCTGGGCTCCAAAGGGATCTATTCAAAAGGTATGTTTCACAACTTTTTCCATTGTTGGTAGATCTTGTAAGGAGTGAGCATAGCTCTGGAGAAGTTCAGCTTGTTTTGAGCAGCATATTTCAATCATGTATAGGCCCAATAATTATTATGCAATAA

Protein sequence

MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASGLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPSTIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGLTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNFVNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDGFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAIKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQPSFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTALSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEETAAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPEEVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLPGEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFIRNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVSWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSVTLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDAVKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKSVGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNFELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYEMVVDDDSSKAPDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNILKDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLVDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIFLALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYIVSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKKLQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRIYLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVSQLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPIIIMQ
Homology
BLAST of MC04g0302 vs. ExPASy Swiss-Prot
Match: F4JSZ5 (Brefeldin A-inhibited guanine nucleotide-exchange protein 1 OS=Arabidopsis thaliana OX=3702 GN=BIG1 PE=2 SV=1)

HSP 1 Score: 2290.0 bits (5933), Expect = 0.0e+00
Identity = 1190/1731 (68.75%), Postives = 1415/1731 (81.74%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60
            MS SQ LGG +RCGR IGPSLDKI+KNAAWRKH+ LVS+CK VLDKL++L ++P DP+S 
Sbjct: 1    MSSSQNLGGATRCGRVIGPSLDKIIKNAAWRKHTFLVSACKSVLDKLEALSDSP-DPSSP 60

Query: 61   LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120
            L GL+ +DA+ VLQPLLL+LD  Y KV EPAL+C FKLFS  L RGE+       +SSP 
Sbjct: 61   LFGLTTSDADAVLQPLLLSLDTGYAKVIEPALDCSFKLFSLSLLRGEV------CSSSPD 120

Query: 121  TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180
            +++YK++ ++CK  G+G+E IEL  LRVLL+AVRSP +LIRGDCL+++VRTCYNVYLGG 
Sbjct: 121  SLLYKLIHAICKVCGIGEESIELAVLRVLLAAVRSPRILIRGDCLLHLVRTCYNVYLGGF 180

Query: 181  TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240
             GTNQICAKSVLAQIM++VF+R E +SMDA ++ ++V++LL  TDKN+NEGNS++ CQ F
Sbjct: 181  NGTNQICAKSVLAQIMLIVFTRSEANSMDASLKTVNVNDLLAITDKNVNEGNSVHICQGF 240

Query: 241  VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG 300
            +N+V+ A E  A    ++     + G +S              TED    G  SKIREDG
Sbjct: 241  INDVITAGEA-APPPDFALVQPPEEGASS--------------TED---EGTGSKIREDG 300

Query: 301  FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAI 360
            F LFKNLCKLSMKFSSQE+ DDQIL+RGK LSLELLKV++DN GP+W S     RFLNAI
Sbjct: 301  FLLFKNLCKLSMKFSSQENTDDQILVRGKTLSLELLKVIIDNGGPIWLS---DERFLNAI 360

Query: 361  KQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQP 420
            KQ LCLSLLKNSALSVM+IFQLQC+IFT+LL K+RSG+K+E+GIFFPMLVLRVLENVLQP
Sbjct: 361  KQLLCLSLLKNSALSVMSIFQLQCAIFTTLLRKYRSGMKSEVGIFFPMLVLRVLENVLQP 420

Query: 421  SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTA 480
            SF+QKMTVL+LL+ I  D  +++DIFVN+DCDV+SPNIFERIVNGLLKTALGPP GS+T 
Sbjct: 421  SFVQKMTVLSLLENICHDPNLIIDIFVNFDCDVESPNIFERIVNGLLKTALGPPPGSSTI 480

Query: 481  LSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEET 540
            LSP QDITFRHESVKCLVSIIK+MGTWMDQQL + D+ LPK LEN+A   +HS  + E+ 
Sbjct: 481  LSPVQDITFRHESVKCLVSIIKAMGTWMDQQLSVGDSLLPKSLENEAPANNHSNSNEEDG 540

Query: 541  AAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPE 600
              +D + + D N ESSD ATLEQRRAYKIE QKG++LFNRKPS+GIEFL+S+KKVG SP+
Sbjct: 541  TTIDHDFHPDLNPESSDAATLEQRRAYKIERQKGVTLFNRKPSKGIEFLISSKKVGNSPD 600

Query: 601  EVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP 660
            EV SFL+NT+GLN T+IGDYLGERE+FP+KVMHAYVDSF+FK M+FGEAIRFFLRGFRLP
Sbjct: 601  EVVSFLRNTTGLNATMIGDYLGEREDFPMKVMHAYVDSFDFKEMNFGEAIRFFLRGFRLP 660

Query: 661  GEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI 720
            GEAQKIDRIMEKFAER+CKCNP SF+ ADTAYVLAYSVIMLNTDAHN MVK+KMTKADFI
Sbjct: 661  GEAQKIDRIMEKFAERFCKCNPNSFSSADTAYVLAYSVIMLNTDAHNIMVKEKMTKADFI 720

Query: 721  RNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS 780
            RNNRGIDDGKDLP+EYLGALYDQ++ NEIKM+SDSSA +S+Q+  +NKLLGLDGILNLV 
Sbjct: 721  RNNRGIDDGKDLPEEYLGALYDQVVINEIKMSSDSSAPESRQSNGLNKLLGLDGILNLVY 780

Query: 781  WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSV 840
            W QTEEKAVGANGLLI+ IQE+F++KSGKSES YH VTDV ILRFMVEV WGPMLAAFSV
Sbjct: 781  WTQTEEKAVGANGLLIKDIQEKFRSKSGKSESAYHVVTDVAILRFMVEVSWGPMLAAFSV 840

Query: 841  TLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDA 900
            TLDQSDD+LA  +CL GFR+AVHVTA+MG+QTQRDAFVTSMAKFT LHCA DMKQKNVDA
Sbjct: 841  TLDQSDDRLAAVECLRGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNLHCAGDMKQKNVDA 900

Query: 901  VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKS 960
            VKAIISIAIEDGN LQ+AWEHI TCLSRIE+LQLLGEGAP DAS+  S+  ET+EK  K+
Sbjct: 901  VKAIISIAIEDGNHLQDAWEHILTCLSRIEHLQLLGEGAPSDASYFAST--ETEEK--KA 960

Query: 961  VGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNF 1020
            +G  +LKKKG LQNP +MAVVRGGSYDS+++G N PG V  +QIN+ I+NLNLLDQIG+F
Sbjct: 961  LGFPNLKKKGALQNPVMMAVVRGGSYDSSTIGPNMPGLVKQDQINNFIANLNLLDQIGSF 1020

Query: 1021 ELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV 1080
            +LN+V+AHSQ L +EAIVAFVKALCKVS++ELQSPTDPRVFSLTKLVE+AHYNMNRIRLV
Sbjct: 1021 QLNNVYAHSQRLKTEAIVAFVKALCKVSMSELQSPTDPRVFSLTKLVEIAHYNMNRIRLV 1080

Query: 1081 WSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV 1140
            WSR+W++LSDFFVSVGLSENLSVAIFVMDSLRQL+MKFLEREELANYNFQNEFLRPFVIV
Sbjct: 1081 WSRIWSILSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANYNFQNEFLRPFVIV 1140

Query: 1141 MQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEK 1200
            MQKSSS EIRELIVRCISQMVLSRV+NVKSGWKSVF VFT AAADERKNIVLLAFETMEK
Sbjct: 1141 MQKSSSAEIRELIVRCISQMVLSRVSNVKSGWKSVFKVFTTAAADERKNIVLLAFETMEK 1200

Query: 1201 IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE 1260
            IVREYF YITETE TTFTDCVRCLITFTNS F SDVSLNAIAFLRFCA+KLA+GGLV  E
Sbjct: 1201 IVREYFSYITETEATTFTDCVRCLITFTNSTFTSDVSLNAIAFLRFCALKLADGGLVWNE 1260

Query: 1261 M---------VVDDDSSKAPDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEV 1320
                      V DD S   P      D D+  SYWVPLL GLSKLTSD RS IRKSSLEV
Sbjct: 1261 KGRSSSPSTPVTDDHS---PSTQNFMDADENISYWVPLLTGLSKLTSDSRSAIRKSSLEV 1320

Query: 1321 LFNILKDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDE----------HPEGS 1380
            LFNILKDHGH+FSR FW+GV ++V++PIF+S++G  E D+   DE          HP   
Sbjct: 1321 LFNILKDHGHIFSRTFWIGVFSSVIYPIFNSVWG--ENDLLSKDEHSSFPSTFSSHPSEV 1380

Query: 1381 TWDSDTCVVAAECLVDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAG 1440
            +WD++T  +AA+ LVDLF+SFF VIRSQL  VV++L G IRSP  GP   GV AL+RLA 
Sbjct: 1381 SWDAETSAMAAQYLVDLFVSFFTVIRSQLSSVVSLLAGLIRSPAQGPTVAGVGALLRLAD 1440

Query: 1441 ELGSRLSGSEWREIFLALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLS 1500
            ELG R S +EW+EIFLA+ EAA+ T+  FMK L+TMDD+          D D  SDQ  S
Sbjct: 1441 ELGDRFSENEWKEIFLAVNEAASLTLSSFMKTLRTMDDIP---------DEDTLSDQDFS 1500

Query: 1501 T-DGLDDDDLQTASYIVSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSI 1560
              D +D+D LQT SY+V+R KSHI +QL ++QV+TDLY+ H Q     ++++ILEI SSI
Sbjct: 1501 NEDDIDEDSLQTMSYVVARTKSHITVQLQVVQVVTDLYRIHQQSLLASHVTVILEILSSI 1560

Query: 1561 STHAQKLNSEIILQKKLQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPT 1620
            S+HA +LNS++ILQKK+++ACSILE+S+PP++HFEN+++Q+YL+ LQ ++ +   ++   
Sbjct: 1561 SSHAHQLNSDLILQKKVRRACSILELSEPPMLHFENDTFQNYLDILQAIVTNNPGVSLEL 1620

Query: 1621 LVESELVAVCEQILRIYLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSAL 1680
             VES+L+ VC QIL++YLKCT  Q +  E      +WILP+G+A KEE AAR+ LVV+ L
Sbjct: 1621 NVESQLMTVCMQILKMYLKCTLFQGDELEETRQPKNWILPMGAASKEEAAARSPLVVAVL 1680

Query: 1681 RVLSGLQRDLFKRYVSQLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1712
            + L  L+RD FKRY    FPLLV+LVRSEHSS +V  VLS++F +C+G ++
Sbjct: 1681 KALRELKRDSFKRYAPNFFPLLVELVRSEHSSSQVPQVLSTVFHTCMGAMM 1685

BLAST of MC04g0302 vs. ExPASy Swiss-Prot
Match: F4JN05 (Brefeldin A-inhibited guanine nucleotide-exchange protein 4 OS=Arabidopsis thaliana OX=3702 GN=BIG4 PE=3 SV=1)

HSP 1 Score: 2137.8 bits (5538), Expect = 0.0e+00
Identity = 1108/1726 (64.19%), Postives = 1362/1726 (78.91%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60
            MS SQTLGG +RCGR IGPSLDKI+KNAAWRKH++LVSSCK VLDKL+SL +   DP+S 
Sbjct: 1    MSTSQTLGGATRCGRIIGPSLDKIIKNAAWRKHTYLVSSCKSVLDKLESLPDDFHDPSSV 60

Query: 61   LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120
            ++GL+ +DA+ VLQP LL+L+ AY KV EP+L+C FKLFS  + RGEI+      +S   
Sbjct: 61   VSGLAASDADSVLQPFLLSLETAYSKVVEPSLDCAFKLFSLSILRGEIQ------SSKQD 120

Query: 121  TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180
            +I++K+V +V K G + +E I+L  LRVLL+AVRSPC+LIRGDCL++VV+TCYN+YLGGL
Sbjct: 121  SILFKLVNAVSKVGAIAEEPIQLAVLRVLLAAVRSPCILIRGDCLLHVVKTCYNIYLGGL 180

Query: 181  TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240
            +GT QICAKSVLAQ+M+V+F+R EEDS+D  ++ I V+ELL FTDK++NEG+S+YFCQ F
Sbjct: 181  SGTTQICAKSVLAQMMLVIFTRSEEDSLDVSVKTIYVNELLTFTDKSVNEGSSVYFCQGF 240

Query: 241  VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKG--ESDVGETEDGAESGGCSKIRE 300
            VNEVM A +G            LQN     +   +       V   E  +E+G  SK+R+
Sbjct: 241  VNEVMAAGQGSPLPPPDVIQILLQNPETETVMTPDSPSFRGYVANGEGDSETGDMSKVRQ 300

Query: 301  DGFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLN 360
            D F LFKNLCKLSM+FSS+E+ DDQI++RGK LSLELLKV++DN G VW +      F+N
Sbjct: 301  DAFLLFKNLCKLSMRFSSKENNDDQIMVRGKTLSLELLKVIIDNGGSVWRT---NESFIN 360

Query: 361  AIKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVL 420
            A+KQYLCLSLLKNSA+S+M+IFQLQC+IF SLL+K RS LKAE+GIFFPM+VLRVLENVL
Sbjct: 361  AVKQYLCLSLLKNSAVSIMSIFQLQCAIFMSLLSKLRSVLKAEIGIFFPMIVLRVLENVL 420

Query: 421  QPSFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGST 480
            QPS+LQKMTVLNLLDK+SQD Q+MVDIFVNYDCDV+S NI ERIVNGLLKTALGPP+GS+
Sbjct: 421  QPSYLQKMTVLNLLDKMSQDPQLMVDIFVNYDCDVESSNILERIVNGLLKTALGPPTGSS 480

Query: 481  TALSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESH-SIISG 540
            T LSPAQD TFR++SVKCLV++ K+MG WMDQQLK+++T  PK  +  AS +S+ S IS 
Sbjct: 481  TTLSPAQDSTFRNDSVKCLVNLAKAMGNWMDQQLKVNETVWPKGSQVYASMDSNASQISE 540

Query: 541  EETAAVDSELNTD-GNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVG 600
             E    D +   D  N E+ D + LEQRRAYKIELQKGISLFNRKPS+G+EFL+STKK+G
Sbjct: 541  LEGTISDCDSQPDTSNPEAYDASMLEQRRAYKIELQKGISLFNRKPSKGVEFLISTKKIG 600

Query: 601  GSPEEVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRG 660
             SPEEVASFL  T+GLN TVIGDYLGER+E PLKVMHAYVDSFNF+  DF EAIRFFLRG
Sbjct: 601  SSPEEVASFLMKTAGLNGTVIGDYLGERDELPLKVMHAYVDSFNFEKKDFVEAIRFFLRG 660

Query: 661  FRLPGEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTK 720
            FRLPGEAQKIDRIMEKFAE Y KCNPGSF  ADTAYVLAYSVIMLNTDAHNNMVKDKMTK
Sbjct: 661  FRLPGEAQKIDRIMEKFAEHYWKCNPGSFTSADTAYVLAYSVIMLNTDAHNNMVKDKMTK 720

Query: 721  ADFIRNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGIL 780
            ADF+RNNRGIDDGKDLP+EYLG+LYD++++ EI+MNSD+ A Q+KQ   +NKLLGLDGIL
Sbjct: 721  ADFVRNNRGIDDGKDLPEEYLGSLYDRVVKEEIRMNSDTLAPQNKQVNGLNKLLGLDGIL 780

Query: 781  NLVSWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLA 840
            NLVSW Q +EK  GANG LIR IQEQF+AK  KSESVYH VTD++ILRF++EV WGPMLA
Sbjct: 781  NLVSWMQPDEKPHGANGRLIRDIQEQFQAKPEKSESVYHTVTDISILRFILEVSWGPMLA 840

Query: 841  AFSVTLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQK 900
            AFSVT+DQSDD+LATS CL GFR+AVHVTA+MG+QTQRDAFVTSMAKFT LHCAADMKQK
Sbjct: 841  AFSVTIDQSDDRLATSLCLQGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNLHCAADMKQK 900

Query: 901  NVDAVKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEK 960
            NVDAVKAII+IAIEDGN L  +WEHI TCLSRIE+LQLLGE +P +  ++ +   E D+K
Sbjct: 901  NVDAVKAIITIAIEDGNHLHGSWEHILTCLSRIEHLQLLGEVSPSEKRYVPTKKAEVDDK 960

Query: 961  TMKSVGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQ 1020
              K++G  +LKK+G  QNP+VMAVVRGGSYDSTSL  + P  VTPEQI   I+NLNLLDQ
Sbjct: 961  --KALGFPNLKKRGSFQNPSVMAVVRGGSYDSTSLVKSVPKLVTPEQIKSFIANLNLLDQ 1020

Query: 1021 IGNFELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNR 1080
            IGNFELNHV+A+SQ LNSEAIV+FVKALCKVS++ELQSPTDPRVFSLTKLVE AHYNMNR
Sbjct: 1021 IGNFELNHVYANSQRLNSEAIVSFVKALCKVSMSELQSPTDPRVFSLTKLVETAHYNMNR 1080

Query: 1081 IRLVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRP 1140
            IRLVWSR+WNVLSDFFVSVGLSENLSVAIFVMDSLRQL+MKFLEREELANY+FQ+EFLRP
Sbjct: 1081 IRLVWSRIWNVLSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANYHFQHEFLRP 1140

Query: 1141 FVIVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFE 1200
            FV+VMQKSSS EIRELIVRC+SQMVLSRV+NVKSGWK+VF VFT AA DERKNIVLLAFE
Sbjct: 1141 FVVVMQKSSSAEIRELIVRCVSQMVLSRVSNVKSGWKNVFTVFTTAALDERKNIVLLAFE 1200

Query: 1201 TMEKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGL 1260
            T+EKIVR++F  I ETE T + DC+RCLITFTNS+F  D+  N I FLRFCA+KL EGGL
Sbjct: 1201 TIEKIVRDHFHCIIETEITVYADCIRCLITFTNSKFEGDIGFNTIEFLRFCALKLEEGGL 1260

Query: 1261 VCYEMVVDDDSSKA----PDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVL 1320
            V  E + ++  S       D  + TD D+  SYW+PLL GL K  SDPR  IRK S+EVL
Sbjct: 1261 VLNEKLKNNTISALKEDFSDTQSFTDLDEQVSYWIPLLTGLCKQVSDPRPAIRKRSIEVL 1320

Query: 1321 FNILKDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHP-------EGSTWDS 1380
            F+IL DHGHLF+R FW G+ ++++ P+F+++  K ++   ES + P       E +TWD 
Sbjct: 1321 FHILMDHGHLFTRPFWTGIFSSIILPVFNNIRSKTDMLFEESVDSPSSASLDTEETTWDV 1380

Query: 1381 DTCVVAAECLVDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGS 1440
            +T  +A + LVDL + FF  +RSQLP VV+I+ GFI+SP  G   +G+S L+ LA  L  
Sbjct: 1381 ETSTLALQLLVDLLVKFFRSVRSQLPSVVSIIVGFIKSPFQGSTGSGISVLLHLADGLAR 1440

Query: 1441 RLSGSEWREIFLALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGL 1500
              S  EWREIFLALKEAA+ T   FMKVL+TMDD+          DV+  S Q ++   L
Sbjct: 1441 SASEDEWREIFLALKEAASLTFAGFMKVLRTMDDIE---------DVETLSGQSVNIGDL 1500

Query: 1501 DDDDLQTASYIVSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQ 1560
            DDD L   SY+VSR K HI +   I++V++DLY+ +    S  ++ I+ +IFS I++HAQ
Sbjct: 1501 DDDSLHIMSYVVSRTKKHIDVLSQIVEVVSDLYRRNQFSLSASHVDILADIFSCIASHAQ 1560

Query: 1561 KLNSEIILQKKLQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESE 1620
            +LN++ +L++K ++ACS+  +++P +++FENE+Y+SY+ FLQ+M+    +++    +ES 
Sbjct: 1561 QLNTDTVLRRKFKRACSVQNLTEPQLLNFENEAYKSYMMFLQDMVTCNPNVSKELDLESR 1620

Query: 1621 LVAVCEQILRIYLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSG 1680
            LV  C +I++IYLKCT  Q + ++   PVL W+LP+ S + EE  ARTSL+VS+L  L  
Sbjct: 1621 LVTECAKIVKIYLKCTDPQQQEQQQRKPVL-WVLPMESDRVEEATARTSLLVSSLEALCS 1680

Query: 1681 LQRDLFKRYVSQLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1712
            L+ +  K++VS  FPLLVDLVR+EH S +V  VLS++ +SCIGPI+
Sbjct: 1681 LEAESLKKHVSSFFPLLVDLVRTEHCSPQVPYVLSNVLKSCIGPIL 1705

BLAST of MC04g0302 vs. ExPASy Swiss-Prot
Match: Q9LZX8 (Brefeldin A-inhibited guanine nucleotide-exchange protein 2 OS=Arabidopsis thaliana OX=3702 GN=BIG2 PE=2 SV=1)

HSP 1 Score: 1598.2 bits (4137), Expect = 0.0e+00
Identity = 877/1796 (48.83%), Postives = 1194/1796 (66.48%), Query Frame = 0

Query: 11   SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPAS----------- 70
            SR  R + P+L+KIVKNA+WRKHS L + CK V+++L+SL ++P   +S           
Sbjct: 8    SRLSRVVTPALEKIVKNASWRKHSKLANECKAVIERLNSLQKSPPPSSSAATDSESESSV 67

Query: 71   -------GLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPE 130
                   G    S  D+E +  PL+ A      K+ EPA++C+ KL + G  RGE     
Sbjct: 68   PGPLNDGGSIEYSLADSELIFSPLINACGTGLAKIIEPAIDCIQKLIAHGYIRGE----S 127

Query: 131  SEANSSPSTIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTC 190
              +  + S +++K+++SVCK   LGDE IEL  L+ LLSA+ S  + I G CL+ VVRTC
Sbjct: 128  DPSGGAESLLLFKLIDSVCKCHDLGDESIELPVLKTLLSAINSISLRIHGKCLLLVVRTC 187

Query: 191  YNVYLGGLTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGN 250
            Y++YLG     NQ  AK+ L QI+V+VF R+E DS   P++ I V+EL+E  +K+  +G 
Sbjct: 188  YDIYLGSKNVVNQTTAKASLIQILVIVFRRMEADSSTVPIQPIVVAELMEPLEKSDADGT 247

Query: 251  SIYFCQNFVNEVMDASEGIAEKKLYSFSSQLQNG------NASPLKVDN----------- 310
               F Q F+ ++M   +G+    +    S   +G        + ++  N           
Sbjct: 248  MTQFVQGFITKIMQDIDGVLNPTMSGSGSGSGSGGQDGAYGTTTVETTNPTDLLDSTDKD 307

Query: 311  -------------------KGESDVGETE--DGAESGGCSKIREDGFHLFKNLCKLSMKF 370
                               KGE   G+ E  D  E    +K+R D   +F+ LCKLSMK 
Sbjct: 308  MLDAKYWEISMYKSALEGRKGELTDGDAERDDDLEVQIENKLRRDACLVFRALCKLSMKA 367

Query: 371  SSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAIKQYLCLSLLKNSAL 430
              +E   D   +RGK+L+LELLK++++NAG V+ +     +F   IKQ+LCLSLLKNSA 
Sbjct: 368  PPKESSADPQSMRGKILALELLKILLENAGAVFRT---SEKFSADIKQFLCLSLLKNSAS 427

Query: 431  SVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQPSFLQKMTVLNLLDK 490
            ++M IFQL CSIF SL+ +FR+GLKAE+G+FFPM+VLRV+ENV QP+F QKM VL  LDK
Sbjct: 428  TLMIIFQLSCSIFISLVARFRAGLKAEIGVFFPMIVLRVVENVAQPNFQQKMIVLRFLDK 487

Query: 491  ISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTALSPAQDITFRHESV 550
            +  DSQI+VDIF+NYDCDV+S NIFER+VNGLLKTA G P G+ T L P Q+   + E++
Sbjct: 488  LCLDSQILVDIFLNYDCDVNSSNIFERMVNGLLKTAQGVPPGTATTLMPPQEAAMKLEAM 547

Query: 551  KCLVSIIKSMGTWMDQQLKLD-DTSLPK--ILENDASPESHSIISGEETAAVD-SELNTD 610
            KCLV+I+KSMG W+++QL+L    SL K  ++E D  P S  + +G    + D S+  ++
Sbjct: 548  KCLVAILKSMGDWLNKQLRLPVSNSLNKSDVIEIDLGPGSPQLANGNADESADGSDTYSE 607

Query: 611  GNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPEEVASFLKNTS 670
             +  +SD   +EQRRAYK+ELQ+GISLFNRKP++GIEFL++  KVG SPEE+A FLK+ S
Sbjct: 608  SSGGTSDALAIEQRRAYKLELQEGISLFNRKPTKGIEFLINAGKVGESPEEIAGFLKDAS 667

Query: 671  GLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLPGEAQKIDRIM 730
            GLN+T+IGDYLGERE+  LKVMHAYVDSF+F+ M+F EAIR FL GFRLPGEAQKIDRIM
Sbjct: 668  GLNKTLIGDYLGEREDLALKVMHAYVDSFDFRGMEFDEAIRTFLEGFRLPGEAQKIDRIM 727

Query: 731  EKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFIRNNRGIDDGK 790
            EKFAERYCKCNP  F  AD+AYVLAYSVIMLNTDAHN MVK+KM+  DFIRNNRGIDDGK
Sbjct: 728  EKFAERYCKCNPKVFTSADSAYVLAYSVIMLNTDAHNPMVKNKMSADDFIRNNRGIDDGK 787

Query: 791  DLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVSWKQTEEKAVG 850
            DLP +Y+ +LY++I ++EIKM  D    Q KQ  + N++LGLDGILN+V  KQ  +    
Sbjct: 788  DLPADYMRSLYERITKHEIKMKEDDLRLQQKQYANSNRMLGLDGILNIVIRKQWGDSYAE 847

Query: 851  ANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSVTLDQSDDKLA 910
             +  L++H+QEQFK K+ KSES Y+A TDV ILRFM+E CW PMLAAFSV LDQSDD + 
Sbjct: 848  TSDDLMKHMQEQFKEKARKSESTYYAATDVVILRFMIEACWAPMLAAFSVPLDQSDDLIV 907

Query: 911  TSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDAVKAIISIAIE 970
             + CL GF HA+H T++M ++T RDAFVTS+AKFT LH  AD+KQ+N++A+KAI+ +A E
Sbjct: 908  INICLEGFHHAIHATSLMSMKTHRDAFVTSLAKFTSLHSPADIKQRNIEAIKAILRLADE 967

Query: 971  DGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDE-KTMKSVGLSSLKKK 1030
            +GN+LQ+AWEHI TC+SR E L LLGEGAPPDA+F  S   E+++ K  K   L  LK+K
Sbjct: 968  EGNYLQDAWEHILTCVSRFEQLHLLGEGAPPDATFFASKQNESEKSKQPKQYILPVLKRK 1027

Query: 1031 G-GLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNFELNHVFAH 1090
            G G    A   V+R GSYDS SLG      V  EQ++ ++SNLNLL+Q+G  E+N VF+ 
Sbjct: 1028 GPGKSQYAATGVLR-GSYDSMSLGGKGSKNVRQEQMSSIVSNLNLLEQVG--EMNQVFSQ 1087

Query: 1091 SQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLVWSRMWNVL 1150
            SQ LNSEAI+ FVKALCKVS+ EL+SP++PRVFSLTK+VE+AHYNMNRIRLVWS +W VL
Sbjct: 1088 SQKLNSEAIIDFVKALCKVSMDELRSPSNPRVFSLTKIVEIAHYNMNRIRLVWSSIWQVL 1147

Query: 1151 SDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIVMQKSSSTE 1210
            S FFV++G SENLS+AIF MDSLRQL+MKFLEREELANYNFQNEF+ PFVIVM++S+  E
Sbjct: 1148 SGFFVTIGCSENLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMTPFVIVMRRSNDVE 1207

Query: 1211 IRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEKIVREYFPY 1270
            IRELI+RC+SQMVLSRVNNVKSGWKS+F+VFT AA D+ KNIV L+FE +EKI+REYFPY
Sbjct: 1208 IRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVFLSFEIIEKIIREYFPY 1267

Query: 1271 ITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYEM-VVDDDS 1330
            ITETETTTFTDCV CL+ FTN+RF+ D+SL++IAFLR+CA KLAEG L           S
Sbjct: 1268 ITETETTTFTDCVNCLVAFTNNRFSKDISLSSIAFLRYCATKLAEGDLNSPSTNKYKGTS 1327

Query: 1331 SKAPDAPTSTDK-----------DDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1390
             K P +   + K           +++  +W PLL+GLS+L+ DPR  IRKS+L+++F+ L
Sbjct: 1328 GKIPQSSLHSGKSGKQENGEIVNNNHLYFWFPLLSGLSELSFDPRPEIRKSALQIMFDTL 1387

Query: 1391 KDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEH-PEGST-----------WD 1450
            ++HGHLFS   W  V  +V+FPIF   Y +  +D +  DE   +GS+           W 
Sbjct: 1388 RNHGHLFSLPLWEKVFESVLFPIFD--YVRHSIDPSGEDESADQGSSGGEVDELDHDAWL 1447

Query: 1451 SDTCVVAAECLVDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELG 1510
             +TC +A + +VDLF+ F+  +   L  V+ +L  FI+ P    A  G++A +RL  +  
Sbjct: 1448 YETCTLALQLVVDLFVKFYTTVNPLLEKVLMLLVSFIKRPHQSLAGIGIAAFVRLMSDAD 1507

Query: 1511 SRLSGSEWREIFLALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDG 1570
               S  +W E+  ALKEAA +T PDF   L           + +  + +  S    +TDG
Sbjct: 1508 GLFSEEKWLEVVSALKEAAKTTCPDFSYFLSEEYVARSQRSALNIQNSNAESAAPTATDG 1567

Query: 1571 LDDDDLQTASYI---VSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSIS 1630
             +++  +TA+++   +S  K    +QLL+IQ + ++Y  +    S  N  ++++    ++
Sbjct: 1568 -NEESQRTATHLYAAISDAKCRAAVQLLLIQAVMEIYNMYRPQLSAKNTLVLVDALHGVA 1627

Query: 1631 THAQKLNSEIILQKKLQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSL---TN 1690
             HA  +NS  IL+ +LQ+   + ++ DPP++  ENESYQ  L FLQN++ D +       
Sbjct: 1628 LHAHGINSNTILRSRLQELGPMTQMQDPPLLRLENESYQICLTFLQNLVADKTKKEEEEE 1687

Query: 1691 PTLVESELVAVCEQILRIYLKCTRT--QAERKETNNPVLHWILPLGSAKKEELAARTSLV 1713
               +ES LV +C+++L  Y++ + +  + + + +      W +PLGS K+ EL+AR  L+
Sbjct: 1688 EEEIESLLVNICQEVLNFYIETSSSAKKLQSESSRASEYRWRIPLGSGKRRELSARAPLI 1747

BLAST of MC04g0302 vs. ExPASy Swiss-Prot
Match: Q9LPC5 (Brefeldin A-inhibited guanine nucleotide-exchange protein 3 OS=Arabidopsis thaliana OX=3702 GN=BIG3 PE=1 SV=1)

HSP 1 Score: 1596.3 bits (4132), Expect = 0.0e+00
Identity = 874/1776 (49.21%), Postives = 1201/1776 (67.62%), Query Frame = 0

Query: 11   SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDS-----------LGEAPLDPA- 70
            SR GR + P+LDK++KNA+WRKHS L   CK V+++L S            G +   P  
Sbjct: 8    SRLGRVVIPALDKVIKNASWRKHSKLAHECKSVIERLRSPENSSPVADSESGSSIPGPLH 67

Query: 71   -SGLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANS 130
              G A  S  ++E +L PL+ A     +K+ +PA++C+ KL + G  RGE +        
Sbjct: 68   DGGAAEYSLAESEIILSPLINASSTGVLKIVDPAVDCIQKLIAHGYVRGEAD----PTGG 127

Query: 131  SPSTIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYL 190
              + ++ K++E++CK   L DEG+EL  L+ LL+AV S  + I GD L+ +VRTCY +YL
Sbjct: 128  PEALLLSKLIETICKCHELDDEGLELLVLKTLLTAVTSISLRIHGDSLLQIVRTCYGIYL 187

Query: 191  GGLTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFC 250
            G     NQ  AK+ L Q+ V+VF R+E DS   P++ I V+EL+E  DK+ ++ ++    
Sbjct: 188  GSRNVVNQATAKASLVQMSVIVFRRMEADSSTVPIQPIVVAELMEPMDKSESDPSTTQSV 247

Query: 251  QNFVNEVMDASEGIAE----KKLY-----SFSSQLQNGNASPLKV--------------- 310
            Q F+ ++M   +G+      K  +     +F + L  G A+P  +               
Sbjct: 248  QGFITKIMQDIDGVFNSANAKGTFGGHDGAFETSLP-GTANPTDLLDSTDKDMLDAKYWE 307

Query: 311  ---------DNKGESDVGETE--DGAESGGCSKIREDGFHLFKNLCKLSMKFSSQEHPDD 370
                       KGE   GE E  D +E    +K+R D F +F+ LCKLSMK   +E P+ 
Sbjct: 308  ISMYKSALEGRKGELADGEVEKDDDSEVQIGNKLRRDAFLVFRALCKLSMKTPPKEDPE- 367

Query: 371  QILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAIKQYLCLSLLKNSALSVMAIFQL 430
              L+RGK+++LELLK++++NAG V+ +     RFL AIKQYLCLSLLKNSA ++M IFQL
Sbjct: 368  --LMRGKIVALELLKILLENAGAVFRT---SDRFLGAIKQYLCLSLLKNSASNLMIIFQL 427

Query: 431  QCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQPSFLQKMTVLNLLDKISQDSQIM 490
             CSI  SL+++FR+GLKAE+G+FFPM+VLRVLENV QP F QKM VL  LDK+  DSQI+
Sbjct: 428  SCSILLSLVSRFRAGLKAEIGVFFPMIVLRVLENVAQPDFQQKMIVLRFLDKLCVDSQIL 487

Query: 491  VDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTALSPAQDITFRHESVKCLVSIIK 550
            VDIF+NYDCDV+S NIFER+VNGLLKTA G P G+ T L P Q+   + E++KCLV++++
Sbjct: 488  VDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGTVTTLLPPQEAAMKLEAMKCLVAVLR 547

Query: 551  SMGTWMDQQLKLDDTSLPKILE---NDASPESHSIISGEETAAVDSELNTDGNSE----S 610
            SMG W+++QL+L D    K+LE    +    SH + +G+          +D  SE    +
Sbjct: 548  SMGDWVNKQLRLPDPYSAKMLEIVDRNLEEGSHPVENGKGDGGHGGFERSDSQSELSSGN 607

Query: 611  SDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPEEVASFLKNTSGLNET 670
            SD   +EQRRAYK+ELQ+GIS+FN+KP +GIEFL+   KVG SPEE+A+FLK+ SGLN+T
Sbjct: 608  SDALAIEQRRAYKLELQEGISIFNQKPKKGIEFLIKANKVGDSPEEIAAFLKDASGLNKT 667

Query: 671  VIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLPGEAQKIDRIMEKFAE 730
            +IGDYLGERE+  LKVMHAYVDSF F+ M+F EAIR FLRGFRLPGEAQKIDRIMEKFAE
Sbjct: 668  LIGDYLGEREDLSLKVMHAYVDSFEFQGMEFDEAIRAFLRGFRLPGEAQKIDRIMEKFAE 727

Query: 731  RYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFIRNNRGIDDGKDLPDE 790
            R+CKCNP  F+ ADTAYVLAYSVI+LNTDAHN MVK KMT   FIRNNRGIDDGKDLP+E
Sbjct: 728  RFCKCNPKDFSSADTAYVLAYSVILLNTDAHNPMVKSKMTADGFIRNNRGIDDGKDLPEE 787

Query: 791  YLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVSWKQTEEKAVGANGLL 850
            YL ALY++I RNEIKM  D    Q KQ T+ ++LLGLD ILN+V  ++ ++  +  +  L
Sbjct: 788  YLRALYERISRNEIKMKDDGLGPQQKQPTNSSRLLGLDTILNIVVPRRGDDMNMETSDDL 847

Query: 851  IRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSVTLDQSDDKLATSQCL 910
            IRH+QE+FK K+ KSESVY+A +DV ILRFMVEVCW PMLAAFSV LDQSDD + T+ CL
Sbjct: 848  IRHMQERFKEKARKSESVYYAASDVIILRFMVEVCWAPMLAAFSVPLDQSDDAVITTLCL 907

Query: 911  LGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDAVKAIISIAIEDGNFL 970
             GF HA+HVT++M L+T RDAFVTS+AKFT LH  AD+KQKN++A+KAI+ +A E+GN+L
Sbjct: 908  EGFHHAIHVTSVMSLKTHRDAFVTSLAKFTSLHSPADIKQKNIEAIKAIVKLAEEEGNYL 967

Query: 971  QEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTM-KSVGLSSLKKKG-GLQ 1030
            Q+AWEHI TC+SR E+L LLGEGAPPDA+F      E+    + K   + ++K++  G  
Sbjct: 968  QDAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQTESGNSPLAKPNSVPAIKERAPGKL 1027

Query: 1031 NPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNFELNHVFAHSQSLN 1090
              A  A++R GSYD + +   +   VT EQ+N+LISNLNLL+Q+G  +++ +F  SQ LN
Sbjct: 1028 QYAASAMIR-GSYDGSGVAGKASNTVTSEQMNNLISNLNLLEQVG--DMSRIFTRSQRLN 1087

Query: 1091 SEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLVWSRMWNVLSDFFV 1150
            SEAI+ FVKALCKVS+ EL+SP+DPRVFSLTK+VE+AHYNMNRIRLVWS +W+VLSDFFV
Sbjct: 1088 SEAIIDFVKALCKVSMDELRSPSDPRVFSLTKIVEIAHYNMNRIRLVWSSIWHVLSDFFV 1147

Query: 1151 SVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIVMQKSSSTEIRELI 1210
            ++G S+NLS+AIF MDSLRQL+MKFLEREELANYNFQNEF++PFV+VM+KS + EIRELI
Sbjct: 1148 TIGCSDNLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMKPFVVVMRKSGAVEIRELI 1207

Query: 1211 VRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEKIVREYFPYITETE 1270
            +RC+SQMVLSRV+NVKSGWKS+F++FT AA D  KNIV L+FE +EKI+R+YFP+ITETE
Sbjct: 1208 IRCVSQMVLSRVDNVKSGWKSMFMIFTTAAHDAHKNIVFLSFEMVEKIIRDYFPHITETE 1267

Query: 1271 TTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAE---GGLVCYEMVVDDDSSK- 1330
            TTTFTDCV CL+ FTN +F  D+SL AIAFL++CA KLAE   G  +     +     K 
Sbjct: 1268 TTTFTDCVNCLVAFTNCKFEKDISLQAIAFLQYCARKLAEGYVGSSLRRNPPLSPQGGKI 1327

Query: 1331 -APDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNILKDHGHLFSRQFW 1390
               D+    + D++   W PLLAGLS+L+ DPR+ IRK +L+VLF+ L++HG  FS   W
Sbjct: 1328 GKQDSGKFLESDEHLYSWFPLLAGLSELSFDPRAEIRKVALKVLFDTLRNHGDHFSLALW 1387

Query: 1391 VGVINTVVFPIFSSLYGKKEVDINESDE--------HPEGSTWDSDTCVVAAECLVDLFI 1450
              V  +V+F IF   Y +++VD +E D           +  +W  +TC +A + +VDLF+
Sbjct: 1388 ERVFESVLFRIFD--YVRQDVDPSEDDSTDQRGYNGEVDQESWLYETCSLALQLVVDLFV 1447

Query: 1451 SFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIFLALK 1510
            +F+  +   L  V+ +    I+ P    A  G++AL+RL  ++G + S  +W E+   +K
Sbjct: 1448 NFYKTVNPLLKKVLMLFVSLIKRPHQSLAGAGIAALVRLMRDVGHQFSNEQWLEVVSCIK 1507

Query: 1511 EAATSTVPDFMKVLK--TMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYIVS 1570
            EAA +T PDF  V     M+DV+         + D  +D   S D L   + Q  + +V+
Sbjct: 1508 EAADATSPDFSYVTSEDLMEDVS---------NEDETNDN--SNDALRRRNRQLHA-VVT 1567

Query: 1571 RMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKKLQ 1630
              KS   +Q+ +IQ +TD+Y  +    + +++ ++ +    I ++A K+N++++L+ KLQ
Sbjct: 1568 DAKSKASIQIFVIQAVTDIYDMYRMSLTANHMLMLFDAMHGIGSNAHKINADLLLRSKLQ 1627

Query: 1631 KACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRIY- 1690
            +  S LE  + P++  ENES+Q+ + FL N++ D     N   +ES L+++C ++L  Y 
Sbjct: 1628 ELGSSLESQEAPLLRLENESFQTCMTFLDNLISDQPVGYNEAEIESHLISLCREVLEFYI 1687

Query: 1691 -LKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVS 1713
             + C++ Q+ R         W +P GS KK+EL AR  LVV+A++ L  +   LFK+ + 
Sbjct: 1688 NISCSKEQSSR---------WAVPSGSGKKKELTARAPLVVAAIQTLGNMGESLFKKNLP 1746

BLAST of MC04g0302 vs. ExPASy Swiss-Prot
Match: F4IXW2 (Brefeldin A-inhibited guanine nucleotide-exchange protein 5 OS=Arabidopsis thaliana OX=3702 GN=BIG5 PE=1 SV=2)

HSP 1 Score: 812.8 bits (2098), Expect = 7.6e-234
Identity = 583/1774 (32.86%), Postives = 902/1774 (50.85%), Query Frame = 0

Query: 64   LSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPSTIV 123
            L   + E VL+PL LA +   +K+ + AL+CL KL +     G+    +   NS+P T +
Sbjct: 99   LGGAEVELVLKPLRLAFETKNLKIFDAALDCLHKLIAYDHLEGD-PGLDGGKNSAPFTDI 158

Query: 124  YKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGLTGT 183
              +V S C      D  + L  L+VLL+AV S    + G+ L+ V+R CYN+ L   +  
Sbjct: 159  LNMVCS-CVDNSSPDSTV-LQVLKVLLTAVASGKFKVHGEPLLGVIRVCYNIALNSKSPI 218

Query: 184  NQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNFVNE 243
            NQ  +K++L Q++ +VF R+E D + A   +     +   T    NE            E
Sbjct: 219  NQATSKAMLTQMISIVFRRMETDIVSASSTVSQEEHVSGDTSSPKNE------------E 278

Query: 244  VMDASEGIAEKKLYSFSSQLQNGNASPLK--------VDNKG----------ESDVGETE 303
            +  A E   E  L    +Q ++   + ++         D KG            D  + +
Sbjct: 279  ITAADENEKEMTLGDALTQAKDTTLASVEELHTLVGGADIKGLEAALDKAVHLEDGKKIK 338

Query: 304  DGAESGGCSKIREDGFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGP 363
             G E    S  + D   +F+ LCK+ MK  S     D++  + ++LSLELL+ +++    
Sbjct: 339  RGIELESMSIGQRDALLVFRTLCKMGMKEDS-----DEVTTKTRILSLELLQGMLEG--- 398

Query: 364  VWHSVTVGFRFLNAIKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIF 423
            V HS T  F F++++K YL  +LL+ S      IFQ    IF+ LL +FR  LK E+GIF
Sbjct: 399  VSHSFTKNFHFIDSVKAYLSYALLRASVSQSSVIFQYASGIFSVLLLRFRDSLKGEIGIF 458

Query: 424  FPMLVLRVLENVLQPSFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNG 483
            FP++VLR L+N   P+  QKM VL +L+K+ +D Q++VD++VNYDCD+++PN+FER+V  
Sbjct: 459  FPIIVLRSLDNSECPND-QKMGVLRMLEKVCKDPQMLVDVYVNYDCDLEAPNLFERMVTT 518

Query: 484  LLKTALGPPSGSTTALSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILEN 543
            L K A G  S        +Q  + +  S++CLV+++KS+  W  ++++ +          
Sbjct: 519  LSKIAQGSQSADPNPAMASQTASVKGSSLQCLVNVLKSLVDW--EKIRRE---------- 578

Query: 544  DASPESHSIISGEETAAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRG 603
                E+ +  + E++A+    + T   S     +  E+ +A+K  ++  IS FNR   +G
Sbjct: 579  ---AENSTRNANEDSASTGEPIET--KSREDVPSNFEKAKAHKSTMEAAISEFNRNSVKG 638

Query: 604  IEFLMSTKKVGGSPEEVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMD 663
            +E+L++ K V  +P  VA FL++TS L++ +IGDYLG+ EEFPL VMHAYVDS  F  M 
Sbjct: 639  VEYLIANKLVERNPASVAQFLRSTSSLSKVMIGDYLGQHEEFPLAVMHAYVDSMKFSEMK 698

Query: 664  FGEAIRFFLRGFRLPGEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDA 723
            F  AIR FL+GFRLPGEAQKIDRIMEKFAERYC  NPG F  ADTAYVLAY+VIMLNTDA
Sbjct: 699  FHSAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDA 758

Query: 724  HNNMVKDKMTKADFIRNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQ-SKQAT 783
            HN MV  KM+K+DF R N   D     P E L  +YD I++ EIK+  D +  + S Q  
Sbjct: 759  HNPMVWPKMSKSDFTRMNATNDPEDCAPTELLEEIYDSIVQEEIKLKDDDTMKKLSSQRP 818

Query: 784  SINKLLGLDGILNLVSWKQ-TEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTIL 843
               +  GL  ILNL   K+ +   A      ++R  QE F+ K G    V+H V  V I+
Sbjct: 819  GGEERGGLVSILNLGLPKRISAADAKSETEDIVRKTQEIFR-KHGVKRGVFHTVEQVDII 878

Query: 844  RFMVEVCWGPMLAAFSVTLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAK 903
            R MVE    P+LAAFSVT++  D+K     C+ GF+  +H+  ++G+ T R AF+TS+ +
Sbjct: 879  RPMVEAVGWPLLAAFSVTMEVGDNKPRILLCMEGFKAGIHIAYVLGMDTMRYAFLTSLVR 938

Query: 904  FTYLHCAADMKQKNVDAVKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDA 963
            FT+LH   +M+ KNV+A++ ++ +   + + LQ+ W  +  C+SR+E +           
Sbjct: 939  FTFLHAPKEMRSKNVEALRILLGLCDSEPDTLQDTWNAVLECVSRLEFI----------- 998

Query: 964  SFLTSSIIETDEKTMKSVGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQ 1023
                                        +  P + A V  GS   +  GV          
Sbjct: 999  ----------------------------ISTPGIAATVMHGSNQISRDGV---------- 1058

Query: 1024 INHLISNLNLLDQIGNFELNHVFAHSQSLNSEAIVAFVKALCKVSIAEL-QSPTDPRVFS 1083
                   +  L ++       VF +S  L SE++V F  ALC VS  EL QSP   RVFS
Sbjct: 1059 -------VQSLKELAGRPAEQVFVNSVKLPSESVVEFFTALCGVSAEELKQSPA--RVFS 1118

Query: 1084 LTKLVEVAHYNMNRIRLVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLERE 1143
            L KLVE+++YN+ RIR+VW+R+W+VL++ FVS G   +  +A++ +DSLRQL MK+LER 
Sbjct: 1119 LQKLVEISYYNIARIRMVWARIWSVLAEHFVSAGSHHDEKIAMYAIDSLRQLGMKYLERA 1178

Query: 1144 ELANYNFQNEFLRPFVIVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAA 1203
            EL N+ FQN+ L+PFVI+M+ + S  IR LIV CI QM+ S+V ++KSGW+SVF++FTAA
Sbjct: 1179 ELTNFTFQNDILKPFVIIMRNTQSQTIRSLIVDCIVQMIKSKVGSIKSGWRSVFMIFTAA 1238

Query: 1204 AADERKNIVLLAFETMEKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIA 1263
            A DE ++IV  +FE +E+++ E+F  +       F DCV CLI F N++ +  +SL AIA
Sbjct: 1239 ADDEVESIVEKSFENVEQVILEHFDQVI---GDCFMDCVNCLIRFANNKASDRISLKAIA 1298

Query: 1264 FLRFCAVKLAEGGLVCYEMVVDDDSSKAPDAPTSTDKDDYASYWVPLLAGLSKLTSDPRS 1323
             LR C  +LAEG       ++     K  D       D    YW P+LAGLS LTSD R 
Sbjct: 1299 LLRICEDRLAEG-------LIPGGVLKPVDGNEDETFDVTEHYWFPMLAGLSDLTSDYRP 1358

Query: 1324 PIRKSSLEVLFNILKDHGHLFSRQFWVGVINTVVFPIFSSL-YGKKEVDINESDEHPEGS 1383
             +R  +LEVLF++L + G+ FS  FW  + + ++FPIF  + +  KE  I+       G 
Sbjct: 1359 EVRNCALEVLFDLLNERGNKFSTPFWESIFHRILFPIFDHVSHAGKESLIS------SGD 1418

Query: 1384 TWDSDTCVVAAECLVDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAG 1443
                +T + + + L +LF +F+  +   LP ++++L    +       S  + AL+ L  
Sbjct: 1419 VKFRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKKSDQTVVSISLGALVHLIE 1478

Query: 1444 ELGSRLSGSEWREIFLALKEAATSTVP---------------------------DFMKVL 1503
              G + S  +W  +  ++++A+ +T P                           D  +V 
Sbjct: 1479 VGGHQFSEGDWDMLLKSIRDASYTTQPLELLNALSFDNPKKNLVLAGDIEADASDSPRVD 1538

Query: 1504 KTMDDVNVPG--ISQSSYDVDV-------------------ASDQGLSTDGLDDDDLQTA 1563
            +  DD+   G   +Q+S  +                      S  G +   +DD +LQ +
Sbjct: 1539 RNPDDIKDNGKVSAQASPRIGTHGTSLESGIPPKADGSEGRPSSSGRAQKDVDDVNLQRS 1598

Query: 1564 S-------------YIVSRMKSHIG----------------------------------- 1623
                           + S+ KS +                                    
Sbjct: 1599 QTFGQRFMDNLFLRNLTSQPKSSVAEVTVPSSPYKHEDPTEPDSREEESPALGAIRGKCI 1658

Query: 1624 MQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKKLQKACSILE 1683
             QLL++  I  + + +           I++I  S    A   NS   L+ ++      + 
Sbjct: 1659 TQLLLLGAINSIQQKYWSNLKTPQKIAIMDILFSFIEFASSYNSYSNLRTRMNH----IP 1718

Query: 1684 ISDPP--VVHFENESYQSYLNFLQNM---LMDTSSLTNPTL---VESELVAVCEQILRIY 1712
               PP  ++  E E    YL+ LQ     L D +S +   L    E +LV+ CEQ+L+  
Sbjct: 1719 TERPPLNLLRQELEGTTIYLDVLQKTTSGLADDASNSEDRLEGAAEEKLVSFCEQVLK-- 1737

BLAST of MC04g0302 vs. NCBI nr
Match: XP_022136256.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 [Momordica charantia])

HSP 1 Score: 3285 bits (8518), Expect = 0.0
Identity = 1707/1714 (99.59%), Postives = 1707/1714 (99.59%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60
            MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG
Sbjct: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60

Query: 61   LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120
            LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS
Sbjct: 61   LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120

Query: 121  TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180
            TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121  TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181  TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240
            TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181  TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240

Query: 241  VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG 300
            VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG
Sbjct: 241  VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG 300

Query: 301  FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAI 360
            FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVW S     RFLNAI
Sbjct: 301  FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWRS---HERFLNAI 360

Query: 361  KQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQP 420
            KQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQP
Sbjct: 361  KQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQP 420

Query: 421  SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTA 480
            SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTA
Sbjct: 421  SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTA 480

Query: 481  LSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEET 540
            LSP QDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEET
Sbjct: 481  LSPXQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEET 540

Query: 541  AAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPE 600
            AAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPE
Sbjct: 541  AAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPE 600

Query: 601  EVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP 660
            EVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP
Sbjct: 601  EVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP 660

Query: 661  GEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI 720
            GEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI
Sbjct: 661  GEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI 720

Query: 721  RNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS 780
            RNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS
Sbjct: 721  RNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS 780

Query: 781  WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSV 840
            WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSV
Sbjct: 781  WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSV 840

Query: 841  TLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDA 900
            TLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDA
Sbjct: 841  TLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDA 900

Query: 901  VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKS 960
            VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKS
Sbjct: 901  VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKS 960

Query: 961  VGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNF 1020
            VGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNF
Sbjct: 961  VGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNF 1020

Query: 1021 ELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV 1080
            ELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV
Sbjct: 1021 ELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV 1080

Query: 1081 WSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV 1140
            WSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV
Sbjct: 1081 WSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV 1140

Query: 1141 MQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEK 1200
            MQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEK
Sbjct: 1141 MQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEK 1200

Query: 1201 IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE 1260
            IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE
Sbjct: 1201 IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE 1260

Query: 1261 MVVDDDSSKAPDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNILKDHG 1320
            MVVDDDSSKAPDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNILKDHG
Sbjct: 1261 MVVDDDSSKAPDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNILKDHG 1320

Query: 1321 HLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLVDLFI 1380
            HLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLVDLFI
Sbjct: 1321 HLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLVDLFI 1380

Query: 1381 SFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIFLALK 1440
            SFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIFLALK
Sbjct: 1381 SFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIFLALK 1440

Query: 1441 EAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYIVSRM 1500
            EAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYIVSRM
Sbjct: 1441 EAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYIVSRM 1500

Query: 1501 KSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKKLQKA 1560
            KSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKKLQKA
Sbjct: 1501 KSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKKLQKA 1560

Query: 1561 CSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRIYLKC 1620
            CSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRIYLKC
Sbjct: 1561 CSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRIYLKC 1620

Query: 1621 TRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVSQLFP 1680
            TRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVSQLFP
Sbjct: 1621 TRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVSQLFP 1680

Query: 1681 LLVDLVRSEHSSGEVQLVLSSIFQSCIGPIIIMQ 1714
            LLVDLVRSEHSSGEVQLVLSSIFQSCIGPIIIMQ
Sbjct: 1681 LLVDLVRSEHSSGEVQLVLSSIFQSCIGPIIIMQ 1711

BLAST of MC04g0302 vs. NCBI nr
Match: XP_038888749.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 1 [Benincasa hispida])

HSP 1 Score: 2999 bits (7775), Expect = 0.0
Identity = 1556/1716 (90.68%), Postives = 1626/1716 (94.76%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60
            MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCK VLDKLDS+ EA  DPAS 
Sbjct: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIKEAAPDPASP 60

Query: 61   LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120
            LAGLSP DAEFVLQPLLLALDAAYVKVAEPALEC+FKLFSRGLFRGEIERP+ EANSS  
Sbjct: 61   LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSSAI 120

Query: 121  TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180
            +IVYKIVESVCKSGGLGDEGIELT LRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121  SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181  TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240
            +GTNQICAKSVL Q+MV+VFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181  SGTNQICAKSVLGQVMVIVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240

Query: 241  VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG 300
            +NEVMDASEGIA+KKLY+F++QLQNG+ASPLK DNKGESD+GETED       SKIREDG
Sbjct: 241  INEVMDASEGIADKKLYAFAAQLQNGHASPLKADNKGESDIGETEDVY-----SKIREDG 300

Query: 301  FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAI 360
            F+LFKNLCKLSMKFSS EHPDDQILLRGK+LSLELLKVVMDNAGPVW       RFLNAI
Sbjct: 301  FYLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRF---NERFLNAI 360

Query: 361  KQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQP 420
            KQ+LCLSLLKNSALS MAIFQLQCSIFTSLLTKFRSGLKAE+GIFFPMLVLRVLENVLQP
Sbjct: 361  KQFLCLSLLKNSALSAMAIFQLQCSIFTSLLTKFRSGLKAEIGIFFPMLVLRVLENVLQP 420

Query: 421  SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTA 480
            SFLQKMTVLNLLDKISQD QIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 
Sbjct: 421  SFLQKMTVLNLLDKISQDPQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTT 480

Query: 481  LSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEET 540
            LSPAQD+TFR ESVKCLVSIIKSMGTWMDQQ+KLDD +L K  ENDASPE+   +SGEET
Sbjct: 481  LSPAQDVTFRLESVKCLVSIIKSMGTWMDQQMKLDDPNLLKTSENDASPENQ--LSGEET 540

Query: 541  AAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPE 600
            AAVDSEL+ DGNSE SD ATLEQRRAYKIELQKGISLFNRKPSRGIEFL+STKKVGGSPE
Sbjct: 541  AAVDSELHPDGNSEFSDAATLEQRRAYKIELQKGISLFNRKPSRGIEFLISTKKVGGSPE 600

Query: 601  EVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP 660
            EVA+FLKNT+GLNETVIGDYLGEREEFPLKVMHAYVDSFNFK MDFGEAIRFFLRGFRLP
Sbjct: 601  EVAAFLKNTNGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKVMDFGEAIRFFLRGFRLP 660

Query: 661  GEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI 720
            GEAQKIDRIMEKFAERYCKCNPGSF  ADTAYVLAYSVIMLNTDAHNNMVK+KMTKADFI
Sbjct: 661  GEAQKIDRIMEKFAERYCKCNPGSFTSADTAYVLAYSVIMLNTDAHNNMVKEKMTKADFI 720

Query: 721  RNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS 780
            RNNRGIDDGKDLPDEYLGALYDQI+RNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS
Sbjct: 721  RNNRGIDDGKDLPDEYLGALYDQIVRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS 780

Query: 781  WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSV 840
            WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDV+ILRFMVEVCWGPMLAAFSV
Sbjct: 781  WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVSILRFMVEVCWGPMLAAFSV 840

Query: 841  TLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDA 900
            TLDQSDDKLATSQCLLGFRHAVHVTA+MGLQTQRDAFVTSMAKFTYLHCAADMKQKNV+A
Sbjct: 841  TLDQSDDKLATSQCLLGFRHAVHVTAVMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVEA 900

Query: 901  VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKS 960
            VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAP DASFLT+S IET+EK  KS
Sbjct: 901  VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPSDASFLTTSSIETEEKAPKS 960

Query: 961  VGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNS-PGPVTPEQINHLISNLNLLDQIGN 1020
            VGLSSLK+KG LQNPAVMAVVRGGSYDSTSLG+NS PGPVTPEQINHLISNLNLL+QIGN
Sbjct: 961  VGLSSLKRKGSLQNPAVMAVVRGGSYDSTSLGMNSSPGPVTPEQINHLISNLNLLEQIGN 1020

Query: 1021 FELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRL 1080
            FELNHVFAHSQSLNSEAIVAFVKALCKV+IAELQSPTDPRVFSLTKLVEVAHYNMNRIRL
Sbjct: 1021 FELNHVFAHSQSLNSEAIVAFVKALCKVAIAELQSPTDPRVFSLTKLVEVAHYNMNRIRL 1080

Query: 1081 VWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVI 1140
            VWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVI
Sbjct: 1081 VWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVI 1140

Query: 1141 VMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETME 1200
            VMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVF+VFTAAAADERKNIVLLAFETME
Sbjct: 1141 VMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFMVFTAAAADERKNIVLLAFETME 1200

Query: 1201 KIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCY 1260
            KIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCY
Sbjct: 1201 KIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCY 1260

Query: 1261 EMVVDDDSSKAPDAPTST----DKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNI 1320
            EMV D+ SS +PD PT T    DKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNI
Sbjct: 1261 EMVGDNVSSNSPDTPTPTPTPTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNI 1320

Query: 1321 LKDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECL 1380
            LKDHGHLFSRQFWVGVIN+VVFPIFSSL+ KKEVD+NE+DE+ EGSTWDSDTC VAA+CL
Sbjct: 1321 LKDHGHLFSRQFWVGVINSVVFPIFSSLHDKKEVDMNENDEYSEGSTWDSDTCAVAADCL 1380

Query: 1381 VDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREI 1440
            VDLF+SFFNVIRSQL GVV ILTGFIRSPIHGPASTGV+ALMRLAG+L +RL+ +EWREI
Sbjct: 1381 VDLFVSFFNVIRSQLAGVVTILTGFIRSPIHGPASTGVAALMRLAGDLANRLTENEWREI 1440

Query: 1441 FLALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASY 1500
            FLALKEAAT TVP F+KVL+TMDD+N+PGISQS YDVDVASDQGLSTDG DDDDLQTASY
Sbjct: 1441 FLALKEAATLTVPGFLKVLRTMDDINIPGISQSYYDVDVASDQGLSTDGRDDDDLQTASY 1500

Query: 1501 IVSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQK 1560
            IVSRMKSHI MQLL+IQVITDLYKNH QPFSE NISIILEIFSSISTHAQKL+S+ ILQK
Sbjct: 1501 IVSRMKSHISMQLLVIQVITDLYKNHTQPFSEGNISIILEIFSSISTHAQKLSSDTILQK 1560

Query: 1561 KLQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILR 1620
            KLQKACSILEISDPPVVHFENESYQSYLNFLQNML +   +TN TL+ESELV VCEQIL 
Sbjct: 1561 KLQKACSILEISDPPVVHFENESYQSYLNFLQNMLANNPLVTNSTLIESELVTVCEQILH 1620

Query: 1621 IYLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYV 1680
            IYLKCT   +E KETN PVLHWILPLGSA+KEELAARTSLVVSALRVL G +RD+FKRY 
Sbjct: 1621 IYLKCTGMPSEGKETNQPVLHWILPLGSARKEELAARTSLVVSALRVLCGFERDIFKRYA 1680

Query: 1681 SQLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1711
            +QLFPLLV+LVRSEHSSGEVQ+VLS IFQSCIGPII
Sbjct: 1681 AQLFPLLVELVRSEHSSGEVQVVLSIIFQSCIGPII 1706

BLAST of MC04g0302 vs. NCBI nr
Match: XP_008451186.1 (PREDICTED: brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 [Cucumis melo])

HSP 1 Score: 2976 bits (7716), Expect = 0.0
Identity = 1544/1715 (90.03%), Postives = 1620/1715 (94.46%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEA-PLDPAS 60
            MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCK VLDKLDS+ EA P DP S
Sbjct: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIAEAVPPDPTS 60

Query: 61   GLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSP 120
             LAGLSP DA+FVLQPLLLALDAAYVKVAEPALEC+FKLFSRGLFRGEIERP+ +ANS+ 
Sbjct: 61   PLAGLSPADADFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGDANSNA 120

Query: 121  STIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGG 180
            ++IVYKIVESVCKSGGLGDEGIELT LRVLLSAVR PCVLIRGDCLV+VVRTCYNVYLGG
Sbjct: 121  NSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVSVVRTCYNVYLGG 180

Query: 181  LTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240
            L+GTNQICAKSVL Q+MV+VFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN
Sbjct: 181  LSGTNQICAKSVLGQVMVIVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240

Query: 241  FVNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIRED 300
            F+NEVMDASEGIA+KKLY FS++LQNG+ASPLKVDNKGESD+GETED      CSKIRED
Sbjct: 241  FINEVMDASEGIADKKLYEFSAKLQNGHASPLKVDNKGESDIGETEDV-----CSKIRED 300

Query: 301  GFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNA 360
            GFHLFKNLCKLSMKFSS EHPDDQILLRGK+LSLELLKVVMDNAGPVW S     RFLNA
Sbjct: 301  GFHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRS---NERFLNA 360

Query: 361  IKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQ 420
            IKQ+LCLSLLKNSALS MAIFQLQC IFTSLLTKFRSGLKAE+GIFFPMLVLRVLENVLQ
Sbjct: 361  IKQFLCLSLLKNSALSAMAIFQLQCCIFTSLLTKFRSGLKAEVGIFFPMLVLRVLENVLQ 420

Query: 421  PSFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480
            PSFLQKMTVLNLLDKISQDSQ MVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT
Sbjct: 421  PSFLQKMTVLNLLDKISQDSQTMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480

Query: 481  ALSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEE 540
             LSPAQDITFR ESVKCLVSIIKSMGTWMDQQ+KLDDT++ K  ENDASPE+   +SGEE
Sbjct: 481  TLSPAQDITFRLESVKCLVSIIKSMGTWMDQQMKLDDTNILKTSENDASPENQ--LSGEE 540

Query: 541  TAAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSP 600
            TAAVDSEL TDGNSE SD ATLEQRRAYKIELQKGISLFNRKPSRGIEFL+STKKVGGSP
Sbjct: 541  TAAVDSELQTDGNSEFSDAATLEQRRAYKIELQKGISLFNRKPSRGIEFLISTKKVGGSP 600

Query: 601  EEVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRL 660
            EEVASFLKNT+GLNETVIGDYLGEREEF LKVMHAYVDSFNFK MDFGEAIRFFLRGFRL
Sbjct: 601  EEVASFLKNTNGLNETVIGDYLGEREEFSLKVMHAYVDSFNFKVMDFGEAIRFFLRGFRL 660

Query: 661  PGEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADF 720
            PGEAQKIDRIMEKFAERYCKCNPGSF  ADTAYVLAYSVIMLNTDAHNNMVK+KMTKADF
Sbjct: 661  PGEAQKIDRIMEKFAERYCKCNPGSFTSADTAYVLAYSVIMLNTDAHNNMVKEKMTKADF 720

Query: 721  IRNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLV 780
            IRNNRGIDDGKDLPDEYLGALYDQI+RNEIKMNSDSSASQSKQATSINKLLG DGILNLV
Sbjct: 721  IRNNRGIDDGKDLPDEYLGALYDQIVRNEIKMNSDSSASQSKQATSINKLLGFDGILNLV 780

Query: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS 840
            SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS
Sbjct: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS 840

Query: 841  VTLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVD 900
            VTLDQSDDKLATSQCLLGFR+AVHVTA+MGLQTQRDAFVTSMAKFTYLHCAADMKQKNV+
Sbjct: 841  VTLDQSDDKLATSQCLLGFRYAVHVTAVMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVE 900

Query: 901  AVKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMK 960
            AVKAIISIAIEDG+FL EAWEHIFTCLSRIENLQLLGEGAP DASFLT+S IET+EK +K
Sbjct: 901  AVKAIISIAIEDGDFLHEAWEHIFTCLSRIENLQLLGEGAPSDASFLTTSNIETEEKALK 960

Query: 961  SVGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNS-PGPVTPEQINHLISNLNLLDQIG 1020
            + GLSSLK+KG LQNPAVMAVVRGGSYDSTSLG NS PGPVTP+QINHLISNL+LL QIG
Sbjct: 961  TAGLSSLKRKGSLQNPAVMAVVRGGSYDSTSLGANSSPGPVTPDQINHLISNLHLLHQIG 1020

Query: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080
            NFELNHVFAHSQSLNSEAIVAFVKALCKV+IAELQSPTDPRVFSLTKLVEVAHYNMNRIR
Sbjct: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVAIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080

Query: 1081 LVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140
            LVWSRMW+VLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV
Sbjct: 1081 LVWSRMWSVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140

Query: 1141 IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETM 1200
            IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVF+VFTAAAADERKNIVLLAFETM
Sbjct: 1141 IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFMVFTAAAADERKNIVLLAFETM 1200

Query: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260
            EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC
Sbjct: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260

Query: 1261 YEMVVDDDSSKAPD--APTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1320
            YEM  D+ SS +PD  +PT TDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL
Sbjct: 1261 YEMAGDNVSSNSPDEPSPTPTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1320

Query: 1321 KDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLV 1380
            KDHGHLFSRQFWVGVIN+VVFPIF+SL+ KKEVD++E+D++ EGSTWD DTC VAA+CLV
Sbjct: 1321 KDHGHLFSRQFWVGVINSVVFPIFNSLHDKKEVDMDENDKYTEGSTWDPDTCAVAADCLV 1380

Query: 1381 DLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIF 1440
            DLFISFFNVIRSQLPGVV ILTGFIRSPI GPASTGV+ALMRLAG+L +RL+ +EWREIF
Sbjct: 1381 DLFISFFNVIRSQLPGVVTILTGFIRSPIQGPASTGVAALMRLAGDLANRLTENEWREIF 1440

Query: 1441 LALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYI 1500
            LALKEAAT TVP F+KVL+TMDD+NVPGISQS YDVD ASDQGLSTDGLDDDDLQTASYI
Sbjct: 1441 LALKEAATLTVPGFLKVLRTMDDINVPGISQSCYDVDAASDQGLSTDGLDDDDLQTASYI 1500

Query: 1501 VSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKK 1560
            VSRMKSHI MQLL+IQVITDLYKNH QPFSE NISIILEIFSSISTHAQKLNS+ +L KK
Sbjct: 1501 VSRMKSHISMQLLVIQVITDLYKNHTQPFSEGNISIILEIFSSISTHAQKLNSDTVLLKK 1560

Query: 1561 LQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRI 1620
            LQKACSILEISDPP+VHFENESYQSYLNFLQNML +   L+N +LVESELV VCEQIL I
Sbjct: 1561 LQKACSILEISDPPMVHFENESYQSYLNFLQNMLANNPLLSNSSLVESELVTVCEQILHI 1620

Query: 1621 YLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVS 1680
            YLKCT T  E KETN PV HWILPLG+A+KEELAARTSLVVSALRVL G +RDLFKRYV 
Sbjct: 1621 YLKCTGTPNELKETNQPVRHWILPLGAARKEELAARTSLVVSALRVLCGFERDLFKRYVP 1680

Query: 1681 QLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1711
            QLFPLLV+LVRSEHSSGEVQ+VLS IFQSCIGPII
Sbjct: 1681 QLFPLLVELVRSEHSSGEVQVVLSIIFQSCIGPII 1705

BLAST of MC04g0302 vs. NCBI nr
Match: XP_004153755.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 [Cucumis sativus] >KGN43373.1 hypothetical protein Csa_020512 [Cucumis sativus])

HSP 1 Score: 2976 bits (7716), Expect = 0.0
Identity = 1543/1719 (89.76%), Postives = 1621/1719 (94.30%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEA-PLDPAS 60
            MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCK VLDKLDS+ EA P DP S
Sbjct: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIAEAVPPDPTS 60

Query: 61   GLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSP 120
             LAGLSP DA+FVLQPLLLALDAAYVKVAEPALEC+FKLFSRGLFRGEIERP+ +ANS+ 
Sbjct: 61   PLAGLSPADADFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGDANSNA 120

Query: 121  STIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGG 180
            S+IVYKIVESVCKSGGLGDEGIELT LRVLLSAVR PCVLIRGDCLVNVVRTCYNVYLGG
Sbjct: 121  SSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGG 180

Query: 181  LTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240
            L+GTNQICAKSVL Q+MV+VFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN
Sbjct: 181  LSGTNQICAKSVLGQVMVIVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240

Query: 241  FVNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIRED 300
            F+NEVMDASEGIA+KKLY FS++LQNG+ASPLKVDNKGESD+GETED      CSKIRED
Sbjct: 241  FINEVMDASEGIADKKLYEFSAKLQNGHASPLKVDNKGESDIGETEDV-----CSKIRED 300

Query: 301  GFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNA 360
            GFHLFKNLCKLSMKFSS EHPDDQIL+RGK+LSLELLKVVMDNAGPVW S     RFLNA
Sbjct: 301  GFHLFKNLCKLSMKFSSPEHPDDQILIRGKILSLELLKVVMDNAGPVWRS---NERFLNA 360

Query: 361  IKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQ 420
            IKQ+LCLSLLKNSALS MAIFQLQC IFTSLLTKFRSGLKAE+GIFFPMLVLRVLENVLQ
Sbjct: 361  IKQFLCLSLLKNSALSAMAIFQLQCCIFTSLLTKFRSGLKAEVGIFFPMLVLRVLENVLQ 420

Query: 421  PSFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480
            PSFLQKMTVLNLLDKISQDSQ MVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT
Sbjct: 421  PSFLQKMTVLNLLDKISQDSQTMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480

Query: 481  ALSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEE 540
             LSPAQDITFR ESVKCLVSIIKSMGTWMDQQ+KLDDT++ K  E+DASPE+   ISGEE
Sbjct: 481  TLSPAQDITFRLESVKCLVSIIKSMGTWMDQQMKLDDTNILKTSESDASPENQ--ISGEE 540

Query: 541  TAAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSP 600
            TAAVDSEL +DGNSE SD ATLEQRRAYKIELQKGISLFNRKPSRGIEFL+STKKVGGSP
Sbjct: 541  TAAVDSELQSDGNSEFSDAATLEQRRAYKIELQKGISLFNRKPSRGIEFLISTKKVGGSP 600

Query: 601  EEVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRL 660
            EEVASFLKNT+GLNETVIGDYLGEREEFPLKVMHAYVDSFNFK MDFGEAIRFFLRGFRL
Sbjct: 601  EEVASFLKNTNGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKVMDFGEAIRFFLRGFRL 660

Query: 661  PGEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADF 720
            PGEAQKIDRIMEKFAERYCKCNP SF  ADTAYVLAYSVIMLNTDAHNNMVK+KMTKADF
Sbjct: 661  PGEAQKIDRIMEKFAERYCKCNPDSFTSADTAYVLAYSVIMLNTDAHNNMVKEKMTKADF 720

Query: 721  IRNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLV 780
            IRNNRGIDDGKDLPDEYLGALYDQI+RNEIKMNSDSSASQSKQATSINKLLGLDGILNLV
Sbjct: 721  IRNNRGIDDGKDLPDEYLGALYDQIVRNEIKMNSDSSASQSKQATSINKLLGLDGILNLV 780

Query: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS 840
            SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEV WGPMLAAFS
Sbjct: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVFWGPMLAAFS 840

Query: 841  VTLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVD 900
            VTLDQSDDKLATSQCLLGFR+AVHVTA+MGLQTQRDAFVTSMAKFTYLHCAADMKQKNV+
Sbjct: 841  VTLDQSDDKLATSQCLLGFRYAVHVTAVMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVE 900

Query: 901  AVKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMK 960
            AVKAIISIAIEDG+FLQEAWEHIFTCLSRIENLQLLGEGAP DASFLT+S IET+EK +K
Sbjct: 901  AVKAIISIAIEDGDFLQEAWEHIFTCLSRIENLQLLGEGAPSDASFLTTSNIETEEKALK 960

Query: 961  SVGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNS-PGPVTPEQINHLISNLNLLDQIG 1020
            + GLSSLK+KG LQNPAVMAVVRGGSYDSTSLG NS PGPVTP+QINHLISNL+LL QIG
Sbjct: 961  TAGLSSLKRKGSLQNPAVMAVVRGGSYDSTSLGANSSPGPVTPDQINHLISNLHLLHQIG 1020

Query: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080
            NFELNHVFAHSQSLNSEAIVAFVKALCKV+IAELQSPTDPRVFSLTKLVEVAHYNMNRIR
Sbjct: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVAIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080

Query: 1081 LVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140
            LVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV
Sbjct: 1081 LVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140

Query: 1141 IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETM 1200
            IVMQKS STEIRELIVRCISQMVLSRVNNVKSGWKSVF+VFTAAAADERKNIVLLAFETM
Sbjct: 1141 IVMQKSGSTEIRELIVRCISQMVLSRVNNVKSGWKSVFMVFTAAAADERKNIVLLAFETM 1200

Query: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260
            EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC
Sbjct: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260

Query: 1261 YEMVVDDDSSKAPD------APTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVL 1320
            YEM  D+ SS +PD       PT TDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVL
Sbjct: 1261 YEMAGDNVSSNSPDEPTPTPTPTPTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVL 1320

Query: 1321 FNILKDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAA 1380
            FNILKDHGHLFSRQFWVGVIN+VVFPIF+SL+ KKEVD++E+D++ EGSTWDSDTC VAA
Sbjct: 1321 FNILKDHGHLFSRQFWVGVINSVVFPIFNSLHDKKEVDMDENDKYTEGSTWDSDTCAVAA 1380

Query: 1381 ECLVDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEW 1440
            +CLVDLF+SFFNVIRSQLPGVVAILTGFIRSPI GPASTGV+ALMRLAG+L +RL+ +EW
Sbjct: 1381 DCLVDLFVSFFNVIRSQLPGVVAILTGFIRSPIQGPASTGVAALMRLAGDLANRLTENEW 1440

Query: 1441 REIFLALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQT 1500
            REIFLALKEAAT TVP F+KVL+TMDD+NVPGISQS YDVD ASDQGLSTDG DDDDLQT
Sbjct: 1441 REIFLALKEAATLTVPGFLKVLRTMDDINVPGISQSCYDVDAASDQGLSTDGFDDDDLQT 1500

Query: 1501 ASYIVSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEII 1560
            ASYIVSRMKSHI MQLL++QVITDLYKNH QPFS+ NISIILEIFSSISTHAQKLNS+ +
Sbjct: 1501 ASYIVSRMKSHISMQLLVLQVITDLYKNHTQPFSQGNISIILEIFSSISTHAQKLNSDTV 1560

Query: 1561 LQKKLQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQ 1620
            LQKKLQKACSILEISDPP+VHFENESYQSYLNFLQNML +   L+N TL+ESELV VC Q
Sbjct: 1561 LQKKLQKACSILEISDPPMVHFENESYQSYLNFLQNMLANNPLLSNSTLIESELVTVCAQ 1620

Query: 1621 ILRIYLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFK 1680
            IL IYLKCT TQ E KETN PV HWILPLG+A+KEELAARTSLVVSALRVL G ++DLFK
Sbjct: 1621 ILHIYLKCTGTQNELKETNQPVQHWILPLGAARKEELAARTSLVVSALRVLCGFEKDLFK 1680

Query: 1681 RYVSQLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1711
            RYV QLFPLLV+LVRSEHSSGEVQ+VLS IFQSCIGPII
Sbjct: 1681 RYVPQLFPLLVELVRSEHSSGEVQVVLSIIFQSCIGPII 1709

BLAST of MC04g0302 vs. NCBI nr
Match: KAA0059594.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 2975 bits (7712), Expect = 0.0
Identity = 1543/1715 (89.97%), Postives = 1619/1715 (94.40%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEA-PLDPAS 60
            MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCK VLDKLDS+ EA P DP S
Sbjct: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIAEAVPPDPTS 60

Query: 61   GLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSP 120
             LAGLSP DA+FVLQPLLLALDAAYVKVAEPALEC+FKLFSRGLFRGEIERP+ +ANS+ 
Sbjct: 61   PLAGLSPADADFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGDANSNA 120

Query: 121  STIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGG 180
            ++IVYKIVESVCKSGGLGDEGIELT LRVLLSAVR PCVLIRGDCLV+VVRTCYNVYLGG
Sbjct: 121  NSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVSVVRTCYNVYLGG 180

Query: 181  LTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240
            L+GTNQICAKSVL Q+MV+VFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN
Sbjct: 181  LSGTNQICAKSVLGQVMVIVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240

Query: 241  FVNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIRED 300
            F+NEVMDASEGIA+KKLY FS++LQNG+ASPLKVDNKGESD+GETED      CSKIRED
Sbjct: 241  FINEVMDASEGIADKKLYEFSAKLQNGHASPLKVDNKGESDIGETEDV-----CSKIRED 300

Query: 301  GFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNA 360
            GFHLFKNLCKLSMKFSS EHPDDQILLRGK+LSLELLKVVMDNAGPVW S     RFLNA
Sbjct: 301  GFHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRS---NERFLNA 360

Query: 361  IKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQ 420
            IKQ+LCLSLLKNSALS MAIFQLQC IFTSLLTKFRSGLKAE+GIFFPMLVLRVLENVLQ
Sbjct: 361  IKQFLCLSLLKNSALSAMAIFQLQCCIFTSLLTKFRSGLKAEVGIFFPMLVLRVLENVLQ 420

Query: 421  PSFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480
            PSFLQKMTVLNLLDKISQDSQ MVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT
Sbjct: 421  PSFLQKMTVLNLLDKISQDSQTMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480

Query: 481  ALSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEE 540
             LSPAQDITFR ESVKCLVSIIKSMGTWMDQQ+KLDDT++ K  ENDASPE+   +SGEE
Sbjct: 481  TLSPAQDITFRLESVKCLVSIIKSMGTWMDQQMKLDDTNILKTSENDASPENQ--LSGEE 540

Query: 541  TAAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSP 600
            TAAVDSEL TDGNSE SD ATLEQRRAYKIELQKGISLFNRKPSRGIEFL+STKKVGGSP
Sbjct: 541  TAAVDSELQTDGNSEFSDAATLEQRRAYKIELQKGISLFNRKPSRGIEFLISTKKVGGSP 600

Query: 601  EEVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRL 660
            EEVASFLKNT+GLNETVIGDYLGEREEF LKVMHAYVDSFNFK MDFGEAIRFFLRGFRL
Sbjct: 601  EEVASFLKNTNGLNETVIGDYLGEREEFSLKVMHAYVDSFNFKVMDFGEAIRFFLRGFRL 660

Query: 661  PGEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADF 720
            PGEAQKIDRIMEKFAERYCKCNPGSF  ADTAYVLAYSVIMLNTDAHNNMVK+KMTKADF
Sbjct: 661  PGEAQKIDRIMEKFAERYCKCNPGSFTSADTAYVLAYSVIMLNTDAHNNMVKEKMTKADF 720

Query: 721  IRNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLV 780
            IRNNRGIDDGKDLPDEYLGALYDQI+RNEIKMNSDSSASQSKQATSINKLLG DGILNLV
Sbjct: 721  IRNNRGIDDGKDLPDEYLGALYDQIVRNEIKMNSDSSASQSKQATSINKLLGFDGILNLV 780

Query: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS 840
            SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS
Sbjct: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS 840

Query: 841  VTLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVD 900
            VTLDQSDDKLATSQCLLGFR+AVHVTA+MGLQTQRDAFVTSMAKFTYLHCAADMKQKNV+
Sbjct: 841  VTLDQSDDKLATSQCLLGFRYAVHVTAVMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVE 900

Query: 901  AVKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMK 960
            AVKAIISIAIEDG+FL EAWEHIFTCLSRIENLQLLGEGAP DASFLT+S IET+EK +K
Sbjct: 901  AVKAIISIAIEDGDFLHEAWEHIFTCLSRIENLQLLGEGAPSDASFLTTSNIETEEKALK 960

Query: 961  SVGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNS-PGPVTPEQINHLISNLNLLDQIG 1020
            + GLSSLK+KG LQNPAVMAVVRGGSYDSTSLG NS PGPVTP+QINHLISNL+LL QIG
Sbjct: 961  TAGLSSLKRKGSLQNPAVMAVVRGGSYDSTSLGANSSPGPVTPDQINHLISNLHLLHQIG 1020

Query: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080
            NFELNHVFAHSQSLNSEAIVAFVKALCKV+IAELQSPTDPRVFSLTKLVEVAHYNMNRIR
Sbjct: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVAIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080

Query: 1081 LVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140
            LVWSRMW+VLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV
Sbjct: 1081 LVWSRMWSVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140

Query: 1141 IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETM 1200
            IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVF+VFTAAAADERKNIVLLAFETM
Sbjct: 1141 IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFMVFTAAAADERKNIVLLAFETM 1200

Query: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260
            EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC
Sbjct: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260

Query: 1261 YEMVVDDDSSKAPD--APTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1320
            YEM  D+ SS +PD  +PT TDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL
Sbjct: 1261 YEMAGDNVSSNSPDEPSPTPTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1320

Query: 1321 KDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLV 1380
            KDHGHLFSRQFWVGVIN+VVFPIF+SL+ KKEVD++E+D++ EGSTWD DTC VAA+CLV
Sbjct: 1321 KDHGHLFSRQFWVGVINSVVFPIFNSLHDKKEVDMDENDKYTEGSTWDPDTCAVAADCLV 1380

Query: 1381 DLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIF 1440
            DLFISFFNVIRSQLPGVV ILTGFIRSPI GPASTGV+ALMRLAG+L +RL+ +EWREIF
Sbjct: 1381 DLFISFFNVIRSQLPGVVTILTGFIRSPIQGPASTGVAALMRLAGDLANRLTENEWREIF 1440

Query: 1441 LALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYI 1500
            LALKEAAT TVP F+KVL+TMDD+NVPGISQS YDVD ASDQG STDGLDDDDLQTASYI
Sbjct: 1441 LALKEAATLTVPGFLKVLRTMDDINVPGISQSCYDVDAASDQGFSTDGLDDDDLQTASYI 1500

Query: 1501 VSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKK 1560
            VSRMKSHI MQLL+IQVITDLYKNH QPFSE NISIILEIFSSISTHAQKLNS+ +L KK
Sbjct: 1501 VSRMKSHISMQLLVIQVITDLYKNHTQPFSEGNISIILEIFSSISTHAQKLNSDTVLLKK 1560

Query: 1561 LQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRI 1620
            LQKACSILEISDPP+VHFENESYQSYLNFLQNML +   L+N +LVESELV VCEQIL I
Sbjct: 1561 LQKACSILEISDPPMVHFENESYQSYLNFLQNMLANNPLLSNSSLVESELVTVCEQILHI 1620

Query: 1621 YLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVS 1680
            YLKCT T  E KETN PV HWILPLG+A+KEELAARTSLVVSALRVL G +RDLFKRYV 
Sbjct: 1621 YLKCTGTPNELKETNQPVRHWILPLGAARKEELAARTSLVVSALRVLCGFERDLFKRYVP 1680

Query: 1681 QLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1711
            QLFPLLV+LVRSEHSSGEVQ+VLS IFQSCIGPII
Sbjct: 1681 QLFPLLVELVRSEHSSGEVQVVLSIIFQSCIGPII 1705

BLAST of MC04g0302 vs. ExPASy TrEMBL
Match: A0A6J1C309 (brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007998 PE=4 SV=1)

HSP 1 Score: 3285 bits (8518), Expect = 0.0
Identity = 1707/1714 (99.59%), Postives = 1707/1714 (99.59%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60
            MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG
Sbjct: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60

Query: 61   LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120
            LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS
Sbjct: 61   LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120

Query: 121  TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180
            TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121  TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181  TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240
            TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181  TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240

Query: 241  VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG 300
            VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG
Sbjct: 241  VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG 300

Query: 301  FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAI 360
            FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVW S     RFLNAI
Sbjct: 301  FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWRS---HERFLNAI 360

Query: 361  KQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQP 420
            KQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQP
Sbjct: 361  KQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQP 420

Query: 421  SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTA 480
            SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTA
Sbjct: 421  SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTA 480

Query: 481  LSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEET 540
            LSP QDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEET
Sbjct: 481  LSPXQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEET 540

Query: 541  AAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPE 600
            AAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPE
Sbjct: 541  AAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPE 600

Query: 601  EVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP 660
            EVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP
Sbjct: 601  EVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP 660

Query: 661  GEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI 720
            GEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI
Sbjct: 661  GEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI 720

Query: 721  RNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS 780
            RNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS
Sbjct: 721  RNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS 780

Query: 781  WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSV 840
            WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSV
Sbjct: 781  WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSV 840

Query: 841  TLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDA 900
            TLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDA
Sbjct: 841  TLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDA 900

Query: 901  VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKS 960
            VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKS
Sbjct: 901  VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKS 960

Query: 961  VGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNF 1020
            VGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNF
Sbjct: 961  VGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNF 1020

Query: 1021 ELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV 1080
            ELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV
Sbjct: 1021 ELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV 1080

Query: 1081 WSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV 1140
            WSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV
Sbjct: 1081 WSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV 1140

Query: 1141 MQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEK 1200
            MQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEK
Sbjct: 1141 MQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEK 1200

Query: 1201 IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE 1260
            IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE
Sbjct: 1201 IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE 1260

Query: 1261 MVVDDDSSKAPDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNILKDHG 1320
            MVVDDDSSKAPDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNILKDHG
Sbjct: 1261 MVVDDDSSKAPDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNILKDHG 1320

Query: 1321 HLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLVDLFI 1380
            HLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLVDLFI
Sbjct: 1321 HLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLVDLFI 1380

Query: 1381 SFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIFLALK 1440
            SFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIFLALK
Sbjct: 1381 SFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIFLALK 1440

Query: 1441 EAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYIVSRM 1500
            EAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYIVSRM
Sbjct: 1441 EAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYIVSRM 1500

Query: 1501 KSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKKLQKA 1560
            KSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKKLQKA
Sbjct: 1501 KSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKKLQKA 1560

Query: 1561 CSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRIYLKC 1620
            CSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRIYLKC
Sbjct: 1561 CSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRIYLKC 1620

Query: 1621 TRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVSQLFP 1680
            TRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVSQLFP
Sbjct: 1621 TRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVSQLFP 1680

Query: 1681 LLVDLVRSEHSSGEVQLVLSSIFQSCIGPIIIMQ 1714
            LLVDLVRSEHSSGEVQLVLSSIFQSCIGPIIIMQ
Sbjct: 1681 LLVDLVRSEHSSGEVQLVLSSIFQSCIGPIIIMQ 1711

BLAST of MC04g0302 vs. ExPASy TrEMBL
Match: A0A0A0K1N6 (SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G027890 PE=4 SV=1)

HSP 1 Score: 2976 bits (7716), Expect = 0.0
Identity = 1543/1719 (89.76%), Postives = 1621/1719 (94.30%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEA-PLDPAS 60
            MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCK VLDKLDS+ EA P DP S
Sbjct: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIAEAVPPDPTS 60

Query: 61   GLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSP 120
             LAGLSP DA+FVLQPLLLALDAAYVKVAEPALEC+FKLFSRGLFRGEIERP+ +ANS+ 
Sbjct: 61   PLAGLSPADADFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGDANSNA 120

Query: 121  STIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGG 180
            S+IVYKIVESVCKSGGLGDEGIELT LRVLLSAVR PCVLIRGDCLVNVVRTCYNVYLGG
Sbjct: 121  SSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGG 180

Query: 181  LTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240
            L+GTNQICAKSVL Q+MV+VFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN
Sbjct: 181  LSGTNQICAKSVLGQVMVIVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240

Query: 241  FVNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIRED 300
            F+NEVMDASEGIA+KKLY FS++LQNG+ASPLKVDNKGESD+GETED      CSKIRED
Sbjct: 241  FINEVMDASEGIADKKLYEFSAKLQNGHASPLKVDNKGESDIGETEDV-----CSKIRED 300

Query: 301  GFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNA 360
            GFHLFKNLCKLSMKFSS EHPDDQIL+RGK+LSLELLKVVMDNAGPVW S     RFLNA
Sbjct: 301  GFHLFKNLCKLSMKFSSPEHPDDQILIRGKILSLELLKVVMDNAGPVWRS---NERFLNA 360

Query: 361  IKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQ 420
            IKQ+LCLSLLKNSALS MAIFQLQC IFTSLLTKFRSGLKAE+GIFFPMLVLRVLENVLQ
Sbjct: 361  IKQFLCLSLLKNSALSAMAIFQLQCCIFTSLLTKFRSGLKAEVGIFFPMLVLRVLENVLQ 420

Query: 421  PSFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480
            PSFLQKMTVLNLLDKISQDSQ MVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT
Sbjct: 421  PSFLQKMTVLNLLDKISQDSQTMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480

Query: 481  ALSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEE 540
             LSPAQDITFR ESVKCLVSIIKSMGTWMDQQ+KLDDT++ K  E+DASPE+   ISGEE
Sbjct: 481  TLSPAQDITFRLESVKCLVSIIKSMGTWMDQQMKLDDTNILKTSESDASPENQ--ISGEE 540

Query: 541  TAAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSP 600
            TAAVDSEL +DGNSE SD ATLEQRRAYKIELQKGISLFNRKPSRGIEFL+STKKVGGSP
Sbjct: 541  TAAVDSELQSDGNSEFSDAATLEQRRAYKIELQKGISLFNRKPSRGIEFLISTKKVGGSP 600

Query: 601  EEVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRL 660
            EEVASFLKNT+GLNETVIGDYLGEREEFPLKVMHAYVDSFNFK MDFGEAIRFFLRGFRL
Sbjct: 601  EEVASFLKNTNGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKVMDFGEAIRFFLRGFRL 660

Query: 661  PGEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADF 720
            PGEAQKIDRIMEKFAERYCKCNP SF  ADTAYVLAYSVIMLNTDAHNNMVK+KMTKADF
Sbjct: 661  PGEAQKIDRIMEKFAERYCKCNPDSFTSADTAYVLAYSVIMLNTDAHNNMVKEKMTKADF 720

Query: 721  IRNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLV 780
            IRNNRGIDDGKDLPDEYLGALYDQI+RNEIKMNSDSSASQSKQATSINKLLGLDGILNLV
Sbjct: 721  IRNNRGIDDGKDLPDEYLGALYDQIVRNEIKMNSDSSASQSKQATSINKLLGLDGILNLV 780

Query: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS 840
            SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEV WGPMLAAFS
Sbjct: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVFWGPMLAAFS 840

Query: 841  VTLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVD 900
            VTLDQSDDKLATSQCLLGFR+AVHVTA+MGLQTQRDAFVTSMAKFTYLHCAADMKQKNV+
Sbjct: 841  VTLDQSDDKLATSQCLLGFRYAVHVTAVMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVE 900

Query: 901  AVKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMK 960
            AVKAIISIAIEDG+FLQEAWEHIFTCLSRIENLQLLGEGAP DASFLT+S IET+EK +K
Sbjct: 901  AVKAIISIAIEDGDFLQEAWEHIFTCLSRIENLQLLGEGAPSDASFLTTSNIETEEKALK 960

Query: 961  SVGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNS-PGPVTPEQINHLISNLNLLDQIG 1020
            + GLSSLK+KG LQNPAVMAVVRGGSYDSTSLG NS PGPVTP+QINHLISNL+LL QIG
Sbjct: 961  TAGLSSLKRKGSLQNPAVMAVVRGGSYDSTSLGANSSPGPVTPDQINHLISNLHLLHQIG 1020

Query: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080
            NFELNHVFAHSQSLNSEAIVAFVKALCKV+IAELQSPTDPRVFSLTKLVEVAHYNMNRIR
Sbjct: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVAIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080

Query: 1081 LVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140
            LVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV
Sbjct: 1081 LVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140

Query: 1141 IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETM 1200
            IVMQKS STEIRELIVRCISQMVLSRVNNVKSGWKSVF+VFTAAAADERKNIVLLAFETM
Sbjct: 1141 IVMQKSGSTEIRELIVRCISQMVLSRVNNVKSGWKSVFMVFTAAAADERKNIVLLAFETM 1200

Query: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260
            EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC
Sbjct: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260

Query: 1261 YEMVVDDDSSKAPD------APTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVL 1320
            YEM  D+ SS +PD       PT TDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVL
Sbjct: 1261 YEMAGDNVSSNSPDEPTPTPTPTPTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVL 1320

Query: 1321 FNILKDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAA 1380
            FNILKDHGHLFSRQFWVGVIN+VVFPIF+SL+ KKEVD++E+D++ EGSTWDSDTC VAA
Sbjct: 1321 FNILKDHGHLFSRQFWVGVINSVVFPIFNSLHDKKEVDMDENDKYTEGSTWDSDTCAVAA 1380

Query: 1381 ECLVDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEW 1440
            +CLVDLF+SFFNVIRSQLPGVVAILTGFIRSPI GPASTGV+ALMRLAG+L +RL+ +EW
Sbjct: 1381 DCLVDLFVSFFNVIRSQLPGVVAILTGFIRSPIQGPASTGVAALMRLAGDLANRLTENEW 1440

Query: 1441 REIFLALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQT 1500
            REIFLALKEAAT TVP F+KVL+TMDD+NVPGISQS YDVD ASDQGLSTDG DDDDLQT
Sbjct: 1441 REIFLALKEAATLTVPGFLKVLRTMDDINVPGISQSCYDVDAASDQGLSTDGFDDDDLQT 1500

Query: 1501 ASYIVSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEII 1560
            ASYIVSRMKSHI MQLL++QVITDLYKNH QPFS+ NISIILEIFSSISTHAQKLNS+ +
Sbjct: 1501 ASYIVSRMKSHISMQLLVLQVITDLYKNHTQPFSQGNISIILEIFSSISTHAQKLNSDTV 1560

Query: 1561 LQKKLQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQ 1620
            LQKKLQKACSILEISDPP+VHFENESYQSYLNFLQNML +   L+N TL+ESELV VC Q
Sbjct: 1561 LQKKLQKACSILEISDPPMVHFENESYQSYLNFLQNMLANNPLLSNSTLIESELVTVCAQ 1620

Query: 1621 ILRIYLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFK 1680
            IL IYLKCT TQ E KETN PV HWILPLG+A+KEELAARTSLVVSALRVL G ++DLFK
Sbjct: 1621 ILHIYLKCTGTQNELKETNQPVQHWILPLGAARKEELAARTSLVVSALRVLCGFEKDLFK 1680

Query: 1681 RYVSQLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1711
            RYV QLFPLLV+LVRSEHSSGEVQ+VLS IFQSCIGPII
Sbjct: 1681 RYVPQLFPLLVELVRSEHSSGEVQVVLSIIFQSCIGPII 1709

BLAST of MC04g0302 vs. ExPASy TrEMBL
Match: A0A1S3BQB3 (brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492558 PE=4 SV=1)

HSP 1 Score: 2976 bits (7716), Expect = 0.0
Identity = 1544/1715 (90.03%), Postives = 1620/1715 (94.46%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEA-PLDPAS 60
            MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCK VLDKLDS+ EA P DP S
Sbjct: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIAEAVPPDPTS 60

Query: 61   GLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSP 120
             LAGLSP DA+FVLQPLLLALDAAYVKVAEPALEC+FKLFSRGLFRGEIERP+ +ANS+ 
Sbjct: 61   PLAGLSPADADFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGDANSNA 120

Query: 121  STIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGG 180
            ++IVYKIVESVCKSGGLGDEGIELT LRVLLSAVR PCVLIRGDCLV+VVRTCYNVYLGG
Sbjct: 121  NSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVSVVRTCYNVYLGG 180

Query: 181  LTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240
            L+GTNQICAKSVL Q+MV+VFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN
Sbjct: 181  LSGTNQICAKSVLGQVMVIVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240

Query: 241  FVNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIRED 300
            F+NEVMDASEGIA+KKLY FS++LQNG+ASPLKVDNKGESD+GETED      CSKIRED
Sbjct: 241  FINEVMDASEGIADKKLYEFSAKLQNGHASPLKVDNKGESDIGETEDV-----CSKIRED 300

Query: 301  GFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNA 360
            GFHLFKNLCKLSMKFSS EHPDDQILLRGK+LSLELLKVVMDNAGPVW S     RFLNA
Sbjct: 301  GFHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRS---NERFLNA 360

Query: 361  IKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQ 420
            IKQ+LCLSLLKNSALS MAIFQLQC IFTSLLTKFRSGLKAE+GIFFPMLVLRVLENVLQ
Sbjct: 361  IKQFLCLSLLKNSALSAMAIFQLQCCIFTSLLTKFRSGLKAEVGIFFPMLVLRVLENVLQ 420

Query: 421  PSFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480
            PSFLQKMTVLNLLDKISQDSQ MVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT
Sbjct: 421  PSFLQKMTVLNLLDKISQDSQTMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480

Query: 481  ALSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEE 540
             LSPAQDITFR ESVKCLVSIIKSMGTWMDQQ+KLDDT++ K  ENDASPE+   +SGEE
Sbjct: 481  TLSPAQDITFRLESVKCLVSIIKSMGTWMDQQMKLDDTNILKTSENDASPENQ--LSGEE 540

Query: 541  TAAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSP 600
            TAAVDSEL TDGNSE SD ATLEQRRAYKIELQKGISLFNRKPSRGIEFL+STKKVGGSP
Sbjct: 541  TAAVDSELQTDGNSEFSDAATLEQRRAYKIELQKGISLFNRKPSRGIEFLISTKKVGGSP 600

Query: 601  EEVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRL 660
            EEVASFLKNT+GLNETVIGDYLGEREEF LKVMHAYVDSFNFK MDFGEAIRFFLRGFRL
Sbjct: 601  EEVASFLKNTNGLNETVIGDYLGEREEFSLKVMHAYVDSFNFKVMDFGEAIRFFLRGFRL 660

Query: 661  PGEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADF 720
            PGEAQKIDRIMEKFAERYCKCNPGSF  ADTAYVLAYSVIMLNTDAHNNMVK+KMTKADF
Sbjct: 661  PGEAQKIDRIMEKFAERYCKCNPGSFTSADTAYVLAYSVIMLNTDAHNNMVKEKMTKADF 720

Query: 721  IRNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLV 780
            IRNNRGIDDGKDLPDEYLGALYDQI+RNEIKMNSDSSASQSKQATSINKLLG DGILNLV
Sbjct: 721  IRNNRGIDDGKDLPDEYLGALYDQIVRNEIKMNSDSSASQSKQATSINKLLGFDGILNLV 780

Query: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS 840
            SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS
Sbjct: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS 840

Query: 841  VTLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVD 900
            VTLDQSDDKLATSQCLLGFR+AVHVTA+MGLQTQRDAFVTSMAKFTYLHCAADMKQKNV+
Sbjct: 841  VTLDQSDDKLATSQCLLGFRYAVHVTAVMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVE 900

Query: 901  AVKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMK 960
            AVKAIISIAIEDG+FL EAWEHIFTCLSRIENLQLLGEGAP DASFLT+S IET+EK +K
Sbjct: 901  AVKAIISIAIEDGDFLHEAWEHIFTCLSRIENLQLLGEGAPSDASFLTTSNIETEEKALK 960

Query: 961  SVGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNS-PGPVTPEQINHLISNLNLLDQIG 1020
            + GLSSLK+KG LQNPAVMAVVRGGSYDSTSLG NS PGPVTP+QINHLISNL+LL QIG
Sbjct: 961  TAGLSSLKRKGSLQNPAVMAVVRGGSYDSTSLGANSSPGPVTPDQINHLISNLHLLHQIG 1020

Query: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080
            NFELNHVFAHSQSLNSEAIVAFVKALCKV+IAELQSPTDPRVFSLTKLVEVAHYNMNRIR
Sbjct: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVAIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080

Query: 1081 LVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140
            LVWSRMW+VLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV
Sbjct: 1081 LVWSRMWSVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140

Query: 1141 IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETM 1200
            IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVF+VFTAAAADERKNIVLLAFETM
Sbjct: 1141 IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFMVFTAAAADERKNIVLLAFETM 1200

Query: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260
            EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC
Sbjct: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260

Query: 1261 YEMVVDDDSSKAPD--APTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1320
            YEM  D+ SS +PD  +PT TDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL
Sbjct: 1261 YEMAGDNVSSNSPDEPSPTPTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1320

Query: 1321 KDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLV 1380
            KDHGHLFSRQFWVGVIN+VVFPIF+SL+ KKEVD++E+D++ EGSTWD DTC VAA+CLV
Sbjct: 1321 KDHGHLFSRQFWVGVINSVVFPIFNSLHDKKEVDMDENDKYTEGSTWDPDTCAVAADCLV 1380

Query: 1381 DLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIF 1440
            DLFISFFNVIRSQLPGVV ILTGFIRSPI GPASTGV+ALMRLAG+L +RL+ +EWREIF
Sbjct: 1381 DLFISFFNVIRSQLPGVVTILTGFIRSPIQGPASTGVAALMRLAGDLANRLTENEWREIF 1440

Query: 1441 LALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYI 1500
            LALKEAAT TVP F+KVL+TMDD+NVPGISQS YDVD ASDQGLSTDGLDDDDLQTASYI
Sbjct: 1441 LALKEAATLTVPGFLKVLRTMDDINVPGISQSCYDVDAASDQGLSTDGLDDDDLQTASYI 1500

Query: 1501 VSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKK 1560
            VSRMKSHI MQLL+IQVITDLYKNH QPFSE NISIILEIFSSISTHAQKLNS+ +L KK
Sbjct: 1501 VSRMKSHISMQLLVIQVITDLYKNHTQPFSEGNISIILEIFSSISTHAQKLNSDTVLLKK 1560

Query: 1561 LQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRI 1620
            LQKACSILEISDPP+VHFENESYQSYLNFLQNML +   L+N +LVESELV VCEQIL I
Sbjct: 1561 LQKACSILEISDPPMVHFENESYQSYLNFLQNMLANNPLLSNSSLVESELVTVCEQILHI 1620

Query: 1621 YLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVS 1680
            YLKCT T  E KETN PV HWILPLG+A+KEELAARTSLVVSALRVL G +RDLFKRYV 
Sbjct: 1621 YLKCTGTPNELKETNQPVRHWILPLGAARKEELAARTSLVVSALRVLCGFERDLFKRYVP 1680

Query: 1681 QLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1711
            QLFPLLV+LVRSEHSSGEVQ+VLS IFQSCIGPII
Sbjct: 1681 QLFPLLVELVRSEHSSGEVQVVLSIIFQSCIGPII 1705

BLAST of MC04g0302 vs. ExPASy TrEMBL
Match: A0A5A7UWX0 (Brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold54G00150 PE=4 SV=1)

HSP 1 Score: 2975 bits (7712), Expect = 0.0
Identity = 1543/1715 (89.97%), Postives = 1619/1715 (94.40%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEA-PLDPAS 60
            MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCK VLDKLDS+ EA P DP S
Sbjct: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIAEAVPPDPTS 60

Query: 61   GLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSP 120
             LAGLSP DA+FVLQPLLLALDAAYVKVAEPALEC+FKLFSRGLFRGEIERP+ +ANS+ 
Sbjct: 61   PLAGLSPADADFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGDANSNA 120

Query: 121  STIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGG 180
            ++IVYKIVESVCKSGGLGDEGIELT LRVLLSAVR PCVLIRGDCLV+VVRTCYNVYLGG
Sbjct: 121  NSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVSVVRTCYNVYLGG 180

Query: 181  LTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240
            L+GTNQICAKSVL Q+MV+VFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN
Sbjct: 181  LSGTNQICAKSVLGQVMVIVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240

Query: 241  FVNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIRED 300
            F+NEVMDASEGIA+KKLY FS++LQNG+ASPLKVDNKGESD+GETED      CSKIRED
Sbjct: 241  FINEVMDASEGIADKKLYEFSAKLQNGHASPLKVDNKGESDIGETEDV-----CSKIRED 300

Query: 301  GFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNA 360
            GFHLFKNLCKLSMKFSS EHPDDQILLRGK+LSLELLKVVMDNAGPVW S     RFLNA
Sbjct: 301  GFHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRS---NERFLNA 360

Query: 361  IKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQ 420
            IKQ+LCLSLLKNSALS MAIFQLQC IFTSLLTKFRSGLKAE+GIFFPMLVLRVLENVLQ
Sbjct: 361  IKQFLCLSLLKNSALSAMAIFQLQCCIFTSLLTKFRSGLKAEVGIFFPMLVLRVLENVLQ 420

Query: 421  PSFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480
            PSFLQKMTVLNLLDKISQDSQ MVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT
Sbjct: 421  PSFLQKMTVLNLLDKISQDSQTMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTT 480

Query: 481  ALSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEE 540
             LSPAQDITFR ESVKCLVSIIKSMGTWMDQQ+KLDDT++ K  ENDASPE+   +SGEE
Sbjct: 481  TLSPAQDITFRLESVKCLVSIIKSMGTWMDQQMKLDDTNILKTSENDASPENQ--LSGEE 540

Query: 541  TAAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSP 600
            TAAVDSEL TDGNSE SD ATLEQRRAYKIELQKGISLFNRKPSRGIEFL+STKKVGGSP
Sbjct: 541  TAAVDSELQTDGNSEFSDAATLEQRRAYKIELQKGISLFNRKPSRGIEFLISTKKVGGSP 600

Query: 601  EEVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRL 660
            EEVASFLKNT+GLNETVIGDYLGEREEF LKVMHAYVDSFNFK MDFGEAIRFFLRGFRL
Sbjct: 601  EEVASFLKNTNGLNETVIGDYLGEREEFSLKVMHAYVDSFNFKVMDFGEAIRFFLRGFRL 660

Query: 661  PGEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADF 720
            PGEAQKIDRIMEKFAERYCKCNPGSF  ADTAYVLAYSVIMLNTDAHNNMVK+KMTKADF
Sbjct: 661  PGEAQKIDRIMEKFAERYCKCNPGSFTSADTAYVLAYSVIMLNTDAHNNMVKEKMTKADF 720

Query: 721  IRNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLV 780
            IRNNRGIDDGKDLPDEYLGALYDQI+RNEIKMNSDSSASQSKQATSINKLLG DGILNLV
Sbjct: 721  IRNNRGIDDGKDLPDEYLGALYDQIVRNEIKMNSDSSASQSKQATSINKLLGFDGILNLV 780

Query: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS 840
            SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS
Sbjct: 781  SWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFS 840

Query: 841  VTLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVD 900
            VTLDQSDDKLATSQCLLGFR+AVHVTA+MGLQTQRDAFVTSMAKFTYLHCAADMKQKNV+
Sbjct: 841  VTLDQSDDKLATSQCLLGFRYAVHVTAVMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVE 900

Query: 901  AVKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMK 960
            AVKAIISIAIEDG+FL EAWEHIFTCLSRIENLQLLGEGAP DASFLT+S IET+EK +K
Sbjct: 901  AVKAIISIAIEDGDFLHEAWEHIFTCLSRIENLQLLGEGAPSDASFLTTSNIETEEKALK 960

Query: 961  SVGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNS-PGPVTPEQINHLISNLNLLDQIG 1020
            + GLSSLK+KG LQNPAVMAVVRGGSYDSTSLG NS PGPVTP+QINHLISNL+LL QIG
Sbjct: 961  TAGLSSLKRKGSLQNPAVMAVVRGGSYDSTSLGANSSPGPVTPDQINHLISNLHLLHQIG 1020

Query: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080
            NFELNHVFAHSQSLNSEAIVAFVKALCKV+IAELQSPTDPRVFSLTKLVEVAHYNMNRIR
Sbjct: 1021 NFELNHVFAHSQSLNSEAIVAFVKALCKVAIAELQSPTDPRVFSLTKLVEVAHYNMNRIR 1080

Query: 1081 LVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140
            LVWSRMW+VLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV
Sbjct: 1081 LVWSRMWSVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFV 1140

Query: 1141 IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETM 1200
            IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVF+VFTAAAADERKNIVLLAFETM
Sbjct: 1141 IVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFMVFTAAAADERKNIVLLAFETM 1200

Query: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260
            EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC
Sbjct: 1201 EKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVC 1260

Query: 1261 YEMVVDDDSSKAPD--APTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1320
            YEM  D+ SS +PD  +PT TDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL
Sbjct: 1261 YEMAGDNVSSNSPDEPSPTPTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1320

Query: 1321 KDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLV 1380
            KDHGHLFSRQFWVGVIN+VVFPIF+SL+ KKEVD++E+D++ EGSTWD DTC VAA+CLV
Sbjct: 1321 KDHGHLFSRQFWVGVINSVVFPIFNSLHDKKEVDMDENDKYTEGSTWDPDTCAVAADCLV 1380

Query: 1381 DLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIF 1440
            DLFISFFNVIRSQLPGVV ILTGFIRSPI GPASTGV+ALMRLAG+L +RL+ +EWREIF
Sbjct: 1381 DLFISFFNVIRSQLPGVVTILTGFIRSPIQGPASTGVAALMRLAGDLANRLTENEWREIF 1440

Query: 1441 LALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYI 1500
            LALKEAAT TVP F+KVL+TMDD+NVPGISQS YDVD ASDQG STDGLDDDDLQTASYI
Sbjct: 1441 LALKEAATLTVPGFLKVLRTMDDINVPGISQSCYDVDAASDQGFSTDGLDDDDLQTASYI 1500

Query: 1501 VSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKK 1560
            VSRMKSHI MQLL+IQVITDLYKNH QPFSE NISIILEIFSSISTHAQKLNS+ +L KK
Sbjct: 1501 VSRMKSHISMQLLVIQVITDLYKNHTQPFSEGNISIILEIFSSISTHAQKLNSDTVLLKK 1560

Query: 1561 LQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRI 1620
            LQKACSILEISDPP+VHFENESYQSYLNFLQNML +   L+N +LVESELV VCEQIL I
Sbjct: 1561 LQKACSILEISDPPMVHFENESYQSYLNFLQNMLANNPLLSNSSLVESELVTVCEQILHI 1620

Query: 1621 YLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVS 1680
            YLKCT T  E KETN PV HWILPLG+A+KEELAARTSLVVSALRVL G +RDLFKRYV 
Sbjct: 1621 YLKCTGTPNELKETNQPVRHWILPLGAARKEELAARTSLVVSALRVLCGFERDLFKRYVP 1680

Query: 1681 QLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1711
            QLFPLLV+LVRSEHSSGEVQ+VLS IFQSCIGPII
Sbjct: 1681 QLFPLLVELVRSEHSSGEVQVVLSIIFQSCIGPII 1705

BLAST of MC04g0302 vs. ExPASy TrEMBL
Match: A0A6J1JLY0 (brefeldin A-inhibited guanine nucleotide-exchange protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111486404 PE=4 SV=1)

HSP 1 Score: 2968 bits (7694), Expect = 0.0
Identity = 1535/1715 (89.50%), Postives = 1611/1715 (93.94%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60
            MSVSQ LGG SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCK VLDKLDS+ EA LDPAS 
Sbjct: 1    MSVSQALGGKSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSISEASLDPASP 60

Query: 61   LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120
            ++GLSP DAEFVLQPLLLALDAAYVKVAEPALEC+FKLFSRGLFRGEIERP+ EANS  S
Sbjct: 61   ISGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120

Query: 121  TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180
            +IV++IVESVCKSGGLGDEGIELT LRVLLSAVR PCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121  SIVFRIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181  TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240
            +GTNQICAKSVL QIM+++FSR+EEDSMD P  IISV+ELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181  SGTNQICAKSVLGQIMIIIFSRMEEDSMDVPASIISVNELLEFTDKNLNEGNSIYFCQNF 240

Query: 241  VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG 300
            +NEVMDASEG+A+KKL+S   QLQNGNASPLK DNKGESDVGETED AESGGCSKIREDG
Sbjct: 241  INEVMDASEGVADKKLFSSWGQLQNGNASPLKADNKGESDVGETEDVAESGGCSKIREDG 300

Query: 301  FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAI 360
            FHLFKNLCKLSMKFSS EHPDDQILLRGK LSLELLKV+MD AGPVW    +  RFLN+I
Sbjct: 301  FHLFKNLCKLSMKFSSPEHPDDQILLRGKTLSLELLKVIMDKAGPVWR---LNERFLNSI 360

Query: 361  KQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQP 420
            KQ+LCLSLLKNSALS MAIFQ+QCSIF SLLTKFRSGLKAE+GIFFPMLVLRVLENVLQP
Sbjct: 361  KQFLCLSLLKNSALSAMAIFQIQCSIFMSLLTKFRSGLKAEIGIFFPMLVLRVLENVLQP 420

Query: 421  SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTA 480
            SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGST+ 
Sbjct: 421  SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTST 480

Query: 481  LSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEET 540
            LSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDT   K  E+D S E+   +SGEET
Sbjct: 481  LSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTYFLKTSESDVSLEN--CLSGEET 540

Query: 541  AAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPE 600
            AAVDSEL+ DGN E SD ATLEQRRAYKIELQKGISLFNRKPSRGIEFL+STKKVGGSPE
Sbjct: 541  AAVDSELHPDGNPEFSDAATLEQRRAYKIELQKGISLFNRKPSRGIEFLISTKKVGGSPE 600

Query: 601  EVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP 660
            EVASFLKNT+GLNE VIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP
Sbjct: 601  EVASFLKNTNGLNERVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP 660

Query: 661  GEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI 720
            GEAQKIDRIMEKFAERYCKC+PGSF  ADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI
Sbjct: 661  GEAQKIDRIMEKFAERYCKCSPGSFTSADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI 720

Query: 721  RNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS 780
            RNNRGIDDGKDLPDEYLGALYDQI+RNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS
Sbjct: 721  RNNRGIDDGKDLPDEYLGALYDQIVRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS 780

Query: 781  WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSV 840
            WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVE CWGPMLAAFSV
Sbjct: 781  WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEACWGPMLAAFSV 840

Query: 841  TLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDA 900
            TLDQSDDKLATSQCLLGFR AVHVTA++GLQTQRDAFVTSMAKFT LHCAADMKQKNV+A
Sbjct: 841  TLDQSDDKLATSQCLLGFRFAVHVTAVLGLQTQRDAFVTSMAKFTNLHCAADMKQKNVEA 900

Query: 901  VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKS 960
            VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSS IET+EK +KS
Sbjct: 901  VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSNIETEEKVLKS 960

Query: 961  VGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNF 1020
             GLSSLKKKGGLQNPAVMAVVRGGSYDSTS G N+PGPVTPEQINHLISNLNLLDQIGNF
Sbjct: 961  AGLSSLKKKGGLQNPAVMAVVRGGSYDSTSHGANTPGPVTPEQINHLISNLNLLDQIGNF 1020

Query: 1021 ELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV 1080
            ELNHVFAHSQSLNSEAIVAFVKALCKV+IAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV
Sbjct: 1021 ELNHVFAHSQSLNSEAIVAFVKALCKVAIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV 1080

Query: 1081 WSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV 1140
            WSRMW+VLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV
Sbjct: 1081 WSRMWSVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV 1140

Query: 1141 MQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEK 1200
            MQKS+STEIRELIVRCISQMVLSRVNNVKSGWKSVF+VFTAAAADERKNIVLLAFETMEK
Sbjct: 1141 MQKSNSTEIRELIVRCISQMVLSRVNNVKSGWKSVFMVFTAAAADERKNIVLLAFETMEK 1200

Query: 1201 IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE 1260
            IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE
Sbjct: 1201 IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE 1260

Query: 1261 MVVDDDSSKAPDAPTS----TDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1320
            M  D+ SS  PDAP S    TDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL
Sbjct: 1261 MTGDNVSSNTPDAPPSIPVPTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1320

Query: 1321 KDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHPEGSTWDSDTCVVAAECLV 1380
            KDHGHLFSRQFWVGVINT+VFPIFSSL+ KKE+D +E+DE+ EG+TWDSDTC VAA+CLV
Sbjct: 1321 KDHGHLFSRQFWVGVINTIVFPIFSSLHDKKEMDEDENDEYSEGTTWDSDTCTVAADCLV 1380

Query: 1381 DLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIF 1440
            DLFISFFNVIRSQLPGVV+ILTG+IRSPI GPASTGV+ALMRLAG+LG+RL+  EWREIF
Sbjct: 1381 DLFISFFNVIRSQLPGVVSILTGYIRSPIQGPASTGVAALMRLAGDLGNRLTEDEWREIF 1440

Query: 1441 LALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYI 1500
            LALK+AAT TVP FMKVL+TMDDVNV GI+QS YDVDVASDQGLS DGLDDDDLQ ASYI
Sbjct: 1441 LALKQAATLTVPGFMKVLRTMDDVNVLGIAQSYYDVDVASDQGLSADGLDDDDLQMASYI 1500

Query: 1501 VSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKK 1560
            VSRMKSHI MQLLIIQVITDLYK+H QPFSE NISIIL+IFSS++TH+QKLNS  +L KK
Sbjct: 1501 VSRMKSHIAMQLLIIQVITDLYKSHTQPFSEANISIILDIFSSVATHSQKLNSNTVLHKK 1560

Query: 1561 LQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRI 1620
            LQKACSILEISDPPVVHFENESY+SYLNFLQNML ++ SLTN TLVESELV VCEQIL I
Sbjct: 1561 LQKACSILEISDPPVVHFENESYRSYLNFLQNMLAESPSLTNATLVESELVVVCEQILHI 1620

Query: 1621 YLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVS 1680
            YLKCT   +E+KE N PVLHWILPLGSAKKEE+AARTSLVVSAL V+   +RDLFKR V 
Sbjct: 1621 YLKCTGVPSEKKEPNQPVLHWILPLGSAKKEEVAARTSLVVSALEVIRRFERDLFKRCVQ 1680

Query: 1681 QLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1711
            +LFPLLVDLVRSEHSSGEVQLVLSSI QSCIGPII
Sbjct: 1681 RLFPLLVDLVRSEHSSGEVQLVLSSILQSCIGPII 1710

BLAST of MC04g0302 vs. TAIR 10
Match: AT4G38200.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 2290.0 bits (5933), Expect = 0.0e+00
Identity = 1190/1731 (68.75%), Postives = 1415/1731 (81.74%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60
            MS SQ LGG +RCGR IGPSLDKI+KNAAWRKH+ LVS+CK VLDKL++L ++P DP+S 
Sbjct: 1    MSSSQNLGGATRCGRVIGPSLDKIIKNAAWRKHTFLVSACKSVLDKLEALSDSP-DPSSP 60

Query: 61   LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120
            L GL+ +DA+ VLQPLLL+LD  Y KV EPAL+C FKLFS  L RGE+       +SSP 
Sbjct: 61   LFGLTTSDADAVLQPLLLSLDTGYAKVIEPALDCSFKLFSLSLLRGEV------CSSSPD 120

Query: 121  TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180
            +++YK++ ++CK  G+G+E IEL  LRVLL+AVRSP +LIRGDCL+++VRTCYNVYLGG 
Sbjct: 121  SLLYKLIHAICKVCGIGEESIELAVLRVLLAAVRSPRILIRGDCLLHLVRTCYNVYLGGF 180

Query: 181  TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240
             GTNQICAKSVLAQIM++VF+R E +SMDA ++ ++V++LL  TDKN+NEGNS++ CQ F
Sbjct: 181  NGTNQICAKSVLAQIMLIVFTRSEANSMDASLKTVNVNDLLAITDKNVNEGNSVHICQGF 240

Query: 241  VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG 300
            +N+V+ A E  A    ++     + G +S              TED    G  SKIREDG
Sbjct: 241  INDVITAGEA-APPPDFALVQPPEEGASS--------------TED---EGTGSKIREDG 300

Query: 301  FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAI 360
            F LFKNLCKLSMKFSSQE+ DDQIL+RGK LSLELLKV++DN GP+W S     RFLNAI
Sbjct: 301  FLLFKNLCKLSMKFSSQENTDDQILVRGKTLSLELLKVIIDNGGPIWLS---DERFLNAI 360

Query: 361  KQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQP 420
            KQ LCLSLLKNSALSVM+IFQLQC+IFT+LL K+RSG+K+E+GIFFPMLVLRVLENVLQP
Sbjct: 361  KQLLCLSLLKNSALSVMSIFQLQCAIFTTLLRKYRSGMKSEVGIFFPMLVLRVLENVLQP 420

Query: 421  SFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTA 480
            SF+QKMTVL+LL+ I  D  +++DIFVN+DCDV+SPNIFERIVNGLLKTALGPP GS+T 
Sbjct: 421  SFVQKMTVLSLLENICHDPNLIIDIFVNFDCDVESPNIFERIVNGLLKTALGPPPGSSTI 480

Query: 481  LSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESHSIISGEET 540
            LSP QDITFRHESVKCLVSIIK+MGTWMDQQL + D+ LPK LEN+A   +HS  + E+ 
Sbjct: 481  LSPVQDITFRHESVKCLVSIIKAMGTWMDQQLSVGDSLLPKSLENEAPANNHSNSNEEDG 540

Query: 541  AAVDSELNTDGNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPE 600
              +D + + D N ESSD ATLEQRRAYKIE QKG++LFNRKPS+GIEFL+S+KKVG SP+
Sbjct: 541  TTIDHDFHPDLNPESSDAATLEQRRAYKIERQKGVTLFNRKPSKGIEFLISSKKVGNSPD 600

Query: 601  EVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLP 660
            EV SFL+NT+GLN T+IGDYLGERE+FP+KVMHAYVDSF+FK M+FGEAIRFFLRGFRLP
Sbjct: 601  EVVSFLRNTTGLNATMIGDYLGEREDFPMKVMHAYVDSFDFKEMNFGEAIRFFLRGFRLP 660

Query: 661  GEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFI 720
            GEAQKIDRIMEKFAER+CKCNP SF+ ADTAYVLAYSVIMLNTDAHN MVK+KMTKADFI
Sbjct: 661  GEAQKIDRIMEKFAERFCKCNPNSFSSADTAYVLAYSVIMLNTDAHNIMVKEKMTKADFI 720

Query: 721  RNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVS 780
            RNNRGIDDGKDLP+EYLGALYDQ++ NEIKM+SDSSA +S+Q+  +NKLLGLDGILNLV 
Sbjct: 721  RNNRGIDDGKDLPEEYLGALYDQVVINEIKMSSDSSAPESRQSNGLNKLLGLDGILNLVY 780

Query: 781  WKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSV 840
            W QTEEKAVGANGLLI+ IQE+F++KSGKSES YH VTDV ILRFMVEV WGPMLAAFSV
Sbjct: 781  WTQTEEKAVGANGLLIKDIQEKFRSKSGKSESAYHVVTDVAILRFMVEVSWGPMLAAFSV 840

Query: 841  TLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDA 900
            TLDQSDD+LA  +CL GFR+AVHVTA+MG+QTQRDAFVTSMAKFT LHCA DMKQKNVDA
Sbjct: 841  TLDQSDDRLAAVECLRGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNLHCAGDMKQKNVDA 900

Query: 901  VKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKS 960
            VKAIISIAIEDGN LQ+AWEHI TCLSRIE+LQLLGEGAP DAS+  S+  ET+EK  K+
Sbjct: 901  VKAIISIAIEDGNHLQDAWEHILTCLSRIEHLQLLGEGAPSDASYFAST--ETEEK--KA 960

Query: 961  VGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNF 1020
            +G  +LKKKG LQNP +MAVVRGGSYDS+++G N PG V  +QIN+ I+NLNLLDQIG+F
Sbjct: 961  LGFPNLKKKGALQNPVMMAVVRGGSYDSSTIGPNMPGLVKQDQINNFIANLNLLDQIGSF 1020

Query: 1021 ELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLV 1080
            +LN+V+AHSQ L +EAIVAFVKALCKVS++ELQSPTDPRVFSLTKLVE+AHYNMNRIRLV
Sbjct: 1021 QLNNVYAHSQRLKTEAIVAFVKALCKVSMSELQSPTDPRVFSLTKLVEIAHYNMNRIRLV 1080

Query: 1081 WSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIV 1140
            WSR+W++LSDFFVSVGLSENLSVAIFVMDSLRQL+MKFLEREELANYNFQNEFLRPFVIV
Sbjct: 1081 WSRIWSILSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANYNFQNEFLRPFVIV 1140

Query: 1141 MQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEK 1200
            MQKSSS EIRELIVRCISQMVLSRV+NVKSGWKSVF VFT AAADERKNIVLLAFETMEK
Sbjct: 1141 MQKSSSAEIRELIVRCISQMVLSRVSNVKSGWKSVFKVFTTAAADERKNIVLLAFETMEK 1200

Query: 1201 IVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYE 1260
            IVREYF YITETE TTFTDCVRCLITFTNS F SDVSLNAIAFLRFCA+KLA+GGLV  E
Sbjct: 1201 IVREYFSYITETEATTFTDCVRCLITFTNSTFTSDVSLNAIAFLRFCALKLADGGLVWNE 1260

Query: 1261 M---------VVDDDSSKAPDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEV 1320
                      V DD S   P      D D+  SYWVPLL GLSKLTSD RS IRKSSLEV
Sbjct: 1261 KGRSSSPSTPVTDDHS---PSTQNFMDADENISYWVPLLTGLSKLTSDSRSAIRKSSLEV 1320

Query: 1321 LFNILKDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDE----------HPEGS 1380
            LFNILKDHGH+FSR FW+GV ++V++PIF+S++G  E D+   DE          HP   
Sbjct: 1321 LFNILKDHGHIFSRTFWIGVFSSVIYPIFNSVWG--ENDLLSKDEHSSFPSTFSSHPSEV 1380

Query: 1381 TWDSDTCVVAAECLVDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAG 1440
            +WD++T  +AA+ LVDLF+SFF VIRSQL  VV++L G IRSP  GP   GV AL+RLA 
Sbjct: 1381 SWDAETSAMAAQYLVDLFVSFFTVIRSQLSSVVSLLAGLIRSPAQGPTVAGVGALLRLAD 1440

Query: 1441 ELGSRLSGSEWREIFLALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLS 1500
            ELG R S +EW+EIFLA+ EAA+ T+  FMK L+TMDD+          D D  SDQ  S
Sbjct: 1441 ELGDRFSENEWKEIFLAVNEAASLTLSSFMKTLRTMDDIP---------DEDTLSDQDFS 1500

Query: 1501 T-DGLDDDDLQTASYIVSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSI 1560
              D +D+D LQT SY+V+R KSHI +QL ++QV+TDLY+ H Q     ++++ILEI SSI
Sbjct: 1501 NEDDIDEDSLQTMSYVVARTKSHITVQLQVVQVVTDLYRIHQQSLLASHVTVILEILSSI 1560

Query: 1561 STHAQKLNSEIILQKKLQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPT 1620
            S+HA +LNS++ILQKK+++ACSILE+S+PP++HFEN+++Q+YL+ LQ ++ +   ++   
Sbjct: 1561 SSHAHQLNSDLILQKKVRRACSILELSEPPMLHFENDTFQNYLDILQAIVTNNPGVSLEL 1620

Query: 1621 LVESELVAVCEQILRIYLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSAL 1680
             VES+L+ VC QIL++YLKCT  Q +  E      +WILP+G+A KEE AAR+ LVV+ L
Sbjct: 1621 NVESQLMTVCMQILKMYLKCTLFQGDELEETRQPKNWILPMGAASKEEAAARSPLVVAVL 1680

Query: 1681 RVLSGLQRDLFKRYVSQLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1712
            + L  L+RD FKRY    FPLLV+LVRSEHSS +V  VLS++F +C+G ++
Sbjct: 1681 KALRELKRDSFKRYAPNFFPLLVELVRSEHSSSQVPQVLSTVFHTCMGAMM 1685

BLAST of MC04g0302 vs. TAIR 10
Match: AT4G35380.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 2137.8 bits (5538), Expect = 0.0e+00
Identity = 1108/1726 (64.19%), Postives = 1362/1726 (78.91%), Query Frame = 0

Query: 1    MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60
            MS SQTLGG +RCGR IGPSLDKI+KNAAWRKH++LVSSCK VLDKL+SL +   DP+S 
Sbjct: 1    MSTSQTLGGATRCGRIIGPSLDKIIKNAAWRKHTYLVSSCKSVLDKLESLPDDFHDPSSV 60

Query: 61   LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120
            ++GL+ +DA+ VLQP LL+L+ AY KV EP+L+C FKLFS  + RGEI+      +S   
Sbjct: 61   VSGLAASDADSVLQPFLLSLETAYSKVVEPSLDCAFKLFSLSILRGEIQ------SSKQD 120

Query: 121  TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180
            +I++K+V +V K G + +E I+L  LRVLL+AVRSPC+LIRGDCL++VV+TCYN+YLGGL
Sbjct: 121  SILFKLVNAVSKVGAIAEEPIQLAVLRVLLAAVRSPCILIRGDCLLHVVKTCYNIYLGGL 180

Query: 181  TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240
            +GT QICAKSVLAQ+M+V+F+R EEDS+D  ++ I V+ELL FTDK++NEG+S+YFCQ F
Sbjct: 181  SGTTQICAKSVLAQMMLVIFTRSEEDSLDVSVKTIYVNELLTFTDKSVNEGSSVYFCQGF 240

Query: 241  VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKG--ESDVGETEDGAESGGCSKIRE 300
            VNEVM A +G            LQN     +   +       V   E  +E+G  SK+R+
Sbjct: 241  VNEVMAAGQGSPLPPPDVIQILLQNPETETVMTPDSPSFRGYVANGEGDSETGDMSKVRQ 300

Query: 301  DGFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLN 360
            D F LFKNLCKLSM+FSS+E+ DDQI++RGK LSLELLKV++DN G VW +      F+N
Sbjct: 301  DAFLLFKNLCKLSMRFSSKENNDDQIMVRGKTLSLELLKVIIDNGGSVWRT---NESFIN 360

Query: 361  AIKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVL 420
            A+KQYLCLSLLKNSA+S+M+IFQLQC+IF SLL+K RS LKAE+GIFFPM+VLRVLENVL
Sbjct: 361  AVKQYLCLSLLKNSAVSIMSIFQLQCAIFMSLLSKLRSVLKAEIGIFFPMIVLRVLENVL 420

Query: 421  QPSFLQKMTVLNLLDKISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGST 480
            QPS+LQKMTVLNLLDK+SQD Q+MVDIFVNYDCDV+S NI ERIVNGLLKTALGPP+GS+
Sbjct: 421  QPSYLQKMTVLNLLDKMSQDPQLMVDIFVNYDCDVESSNILERIVNGLLKTALGPPTGSS 480

Query: 481  TALSPAQDITFRHESVKCLVSIIKSMGTWMDQQLKLDDTSLPKILENDASPESH-SIISG 540
            T LSPAQD TFR++SVKCLV++ K+MG WMDQQLK+++T  PK  +  AS +S+ S IS 
Sbjct: 481  TTLSPAQDSTFRNDSVKCLVNLAKAMGNWMDQQLKVNETVWPKGSQVYASMDSNASQISE 540

Query: 541  EETAAVDSELNTD-GNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVG 600
             E    D +   D  N E+ D + LEQRRAYKIELQKGISLFNRKPS+G+EFL+STKK+G
Sbjct: 541  LEGTISDCDSQPDTSNPEAYDASMLEQRRAYKIELQKGISLFNRKPSKGVEFLISTKKIG 600

Query: 601  GSPEEVASFLKNTSGLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRG 660
             SPEEVASFL  T+GLN TVIGDYLGER+E PLKVMHAYVDSFNF+  DF EAIRFFLRG
Sbjct: 601  SSPEEVASFLMKTAGLNGTVIGDYLGERDELPLKVMHAYVDSFNFEKKDFVEAIRFFLRG 660

Query: 661  FRLPGEAQKIDRIMEKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTK 720
            FRLPGEAQKIDRIMEKFAE Y KCNPGSF  ADTAYVLAYSVIMLNTDAHNNMVKDKMTK
Sbjct: 661  FRLPGEAQKIDRIMEKFAEHYWKCNPGSFTSADTAYVLAYSVIMLNTDAHNNMVKDKMTK 720

Query: 721  ADFIRNNRGIDDGKDLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGIL 780
            ADF+RNNRGIDDGKDLP+EYLG+LYD++++ EI+MNSD+ A Q+KQ   +NKLLGLDGIL
Sbjct: 721  ADFVRNNRGIDDGKDLPEEYLGSLYDRVVKEEIRMNSDTLAPQNKQVNGLNKLLGLDGIL 780

Query: 781  NLVSWKQTEEKAVGANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLA 840
            NLVSW Q +EK  GANG LIR IQEQF+AK  KSESVYH VTD++ILRF++EV WGPMLA
Sbjct: 781  NLVSWMQPDEKPHGANGRLIRDIQEQFQAKPEKSESVYHTVTDISILRFILEVSWGPMLA 840

Query: 841  AFSVTLDQSDDKLATSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQK 900
            AFSVT+DQSDD+LATS CL GFR+AVHVTA+MG+QTQRDAFVTSMAKFT LHCAADMKQK
Sbjct: 841  AFSVTIDQSDDRLATSLCLQGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNLHCAADMKQK 900

Query: 901  NVDAVKAIISIAIEDGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEK 960
            NVDAVKAII+IAIEDGN L  +WEHI TCLSRIE+LQLLGE +P +  ++ +   E D+K
Sbjct: 901  NVDAVKAIITIAIEDGNHLHGSWEHILTCLSRIEHLQLLGEVSPSEKRYVPTKKAEVDDK 960

Query: 961  TMKSVGLSSLKKKGGLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQ 1020
              K++G  +LKK+G  QNP+VMAVVRGGSYDSTSL  + P  VTPEQI   I+NLNLLDQ
Sbjct: 961  --KALGFPNLKKRGSFQNPSVMAVVRGGSYDSTSLVKSVPKLVTPEQIKSFIANLNLLDQ 1020

Query: 1021 IGNFELNHVFAHSQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNR 1080
            IGNFELNHV+A+SQ LNSEAIV+FVKALCKVS++ELQSPTDPRVFSLTKLVE AHYNMNR
Sbjct: 1021 IGNFELNHVYANSQRLNSEAIVSFVKALCKVSMSELQSPTDPRVFSLTKLVETAHYNMNR 1080

Query: 1081 IRLVWSRMWNVLSDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRP 1140
            IRLVWSR+WNVLSDFFVSVGLSENLSVAIFVMDSLRQL+MKFLEREELANY+FQ+EFLRP
Sbjct: 1081 IRLVWSRIWNVLSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANYHFQHEFLRP 1140

Query: 1141 FVIVMQKSSSTEIRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFE 1200
            FV+VMQKSSS EIRELIVRC+SQMVLSRV+NVKSGWK+VF VFT AA DERKNIVLLAFE
Sbjct: 1141 FVVVMQKSSSAEIRELIVRCVSQMVLSRVSNVKSGWKNVFTVFTTAALDERKNIVLLAFE 1200

Query: 1201 TMEKIVREYFPYITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGL 1260
            T+EKIVR++F  I ETE T + DC+RCLITFTNS+F  D+  N I FLRFCA+KL EGGL
Sbjct: 1201 TIEKIVRDHFHCIIETEITVYADCIRCLITFTNSKFEGDIGFNTIEFLRFCALKLEEGGL 1260

Query: 1261 VCYEMVVDDDSSKA----PDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVL 1320
            V  E + ++  S       D  + TD D+  SYW+PLL GL K  SDPR  IRK S+EVL
Sbjct: 1261 VLNEKLKNNTISALKEDFSDTQSFTDLDEQVSYWIPLLTGLCKQVSDPRPAIRKRSIEVL 1320

Query: 1321 FNILKDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEHP-------EGSTWDS 1380
            F+IL DHGHLF+R FW G+ ++++ P+F+++  K ++   ES + P       E +TWD 
Sbjct: 1321 FHILMDHGHLFTRPFWTGIFSSIILPVFNNIRSKTDMLFEESVDSPSSASLDTEETTWDV 1380

Query: 1381 DTCVVAAECLVDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGS 1440
            +T  +A + LVDL + FF  +RSQLP VV+I+ GFI+SP  G   +G+S L+ LA  L  
Sbjct: 1381 ETSTLALQLLVDLLVKFFRSVRSQLPSVVSIIVGFIKSPFQGSTGSGISVLLHLADGLAR 1440

Query: 1441 RLSGSEWREIFLALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDGL 1500
              S  EWREIFLALKEAA+ T   FMKVL+TMDD+          DV+  S Q ++   L
Sbjct: 1441 SASEDEWREIFLALKEAASLTFAGFMKVLRTMDDIE---------DVETLSGQSVNIGDL 1500

Query: 1501 DDDDLQTASYIVSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQ 1560
            DDD L   SY+VSR K HI +   I++V++DLY+ +    S  ++ I+ +IFS I++HAQ
Sbjct: 1501 DDDSLHIMSYVVSRTKKHIDVLSQIVEVVSDLYRRNQFSLSASHVDILADIFSCIASHAQ 1560

Query: 1561 KLNSEIILQKKLQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESE 1620
            +LN++ +L++K ++ACS+  +++P +++FENE+Y+SY+ FLQ+M+    +++    +ES 
Sbjct: 1561 QLNTDTVLRRKFKRACSVQNLTEPQLLNFENEAYKSYMMFLQDMVTCNPNVSKELDLESR 1620

Query: 1621 LVAVCEQILRIYLKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSG 1680
            LV  C +I++IYLKCT  Q + ++   PVL W+LP+ S + EE  ARTSL+VS+L  L  
Sbjct: 1621 LVTECAKIVKIYLKCTDPQQQEQQQRKPVL-WVLPMESDRVEEATARTSLLVSSLEALCS 1680

Query: 1681 LQRDLFKRYVSQLFPLLVDLVRSEHSSGEVQLVLSSIFQSCIGPII 1712
            L+ +  K++VS  FPLLVDLVR+EH S +V  VLS++ +SCIGPI+
Sbjct: 1681 LEAESLKKHVSSFFPLLVDLVRTEHCSPQVPYVLSNVLKSCIGPIL 1705

BLAST of MC04g0302 vs. TAIR 10
Match: AT3G60860.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 1598.2 bits (4137), Expect = 0.0e+00
Identity = 877/1796 (48.83%), Postives = 1194/1796 (66.48%), Query Frame = 0

Query: 11   SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPAS----------- 70
            SR  R + P+L+KIVKNA+WRKHS L + CK V+++L+SL ++P   +S           
Sbjct: 8    SRLSRVVTPALEKIVKNASWRKHSKLANECKAVIERLNSLQKSPPPSSSAATDSESESSV 67

Query: 71   -------GLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPE 130
                   G    S  D+E +  PL+ A      K+ EPA++C+ KL + G  RGE     
Sbjct: 68   PGPLNDGGSIEYSLADSELIFSPLINACGTGLAKIIEPAIDCIQKLIAHGYIRGE----S 127

Query: 131  SEANSSPSTIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTC 190
              +  + S +++K+++SVCK   LGDE IEL  L+ LLSA+ S  + I G CL+ VVRTC
Sbjct: 128  DPSGGAESLLLFKLIDSVCKCHDLGDESIELPVLKTLLSAINSISLRIHGKCLLLVVRTC 187

Query: 191  YNVYLGGLTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGN 250
            Y++YLG     NQ  AK+ L QI+V+VF R+E DS   P++ I V+EL+E  +K+  +G 
Sbjct: 188  YDIYLGSKNVVNQTTAKASLIQILVIVFRRMEADSSTVPIQPIVVAELMEPLEKSDADGT 247

Query: 251  SIYFCQNFVNEVMDASEGIAEKKLYSFSSQLQNG------NASPLKVDN----------- 310
               F Q F+ ++M   +G+    +    S   +G        + ++  N           
Sbjct: 248  MTQFVQGFITKIMQDIDGVLNPTMSGSGSGSGSGGQDGAYGTTTVETTNPTDLLDSTDKD 307

Query: 311  -------------------KGESDVGETE--DGAESGGCSKIREDGFHLFKNLCKLSMKF 370
                               KGE   G+ E  D  E    +K+R D   +F+ LCKLSMK 
Sbjct: 308  MLDAKYWEISMYKSALEGRKGELTDGDAERDDDLEVQIENKLRRDACLVFRALCKLSMKA 367

Query: 371  SSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAIKQYLCLSLLKNSAL 430
              +E   D   +RGK+L+LELLK++++NAG V+ +     +F   IKQ+LCLSLLKNSA 
Sbjct: 368  PPKESSADPQSMRGKILALELLKILLENAGAVFRT---SEKFSADIKQFLCLSLLKNSAS 427

Query: 431  SVMAIFQLQCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQPSFLQKMTVLNLLDK 490
            ++M IFQL CSIF SL+ +FR+GLKAE+G+FFPM+VLRV+ENV QP+F QKM VL  LDK
Sbjct: 428  TLMIIFQLSCSIFISLVARFRAGLKAEIGVFFPMIVLRVVENVAQPNFQQKMIVLRFLDK 487

Query: 491  ISQDSQIMVDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTALSPAQDITFRHESV 550
            +  DSQI+VDIF+NYDCDV+S NIFER+VNGLLKTA G P G+ T L P Q+   + E++
Sbjct: 488  LCLDSQILVDIFLNYDCDVNSSNIFERMVNGLLKTAQGVPPGTATTLMPPQEAAMKLEAM 547

Query: 551  KCLVSIIKSMGTWMDQQLKLD-DTSLPK--ILENDASPESHSIISGEETAAVD-SELNTD 610
            KCLV+I+KSMG W+++QL+L    SL K  ++E D  P S  + +G    + D S+  ++
Sbjct: 548  KCLVAILKSMGDWLNKQLRLPVSNSLNKSDVIEIDLGPGSPQLANGNADESADGSDTYSE 607

Query: 611  GNSESSDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPEEVASFLKNTS 670
             +  +SD   +EQRRAYK+ELQ+GISLFNRKP++GIEFL++  KVG SPEE+A FLK+ S
Sbjct: 608  SSGGTSDALAIEQRRAYKLELQEGISLFNRKPTKGIEFLINAGKVGESPEEIAGFLKDAS 667

Query: 671  GLNETVIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLPGEAQKIDRIM 730
            GLN+T+IGDYLGERE+  LKVMHAYVDSF+F+ M+F EAIR FL GFRLPGEAQKIDRIM
Sbjct: 668  GLNKTLIGDYLGEREDLALKVMHAYVDSFDFRGMEFDEAIRTFLEGFRLPGEAQKIDRIM 727

Query: 731  EKFAERYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFIRNNRGIDDGK 790
            EKFAERYCKCNP  F  AD+AYVLAYSVIMLNTDAHN MVK+KM+  DFIRNNRGIDDGK
Sbjct: 728  EKFAERYCKCNPKVFTSADSAYVLAYSVIMLNTDAHNPMVKNKMSADDFIRNNRGIDDGK 787

Query: 791  DLPDEYLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVSWKQTEEKAVG 850
            DLP +Y+ +LY++I ++EIKM  D    Q KQ  + N++LGLDGILN+V  KQ  +    
Sbjct: 788  DLPADYMRSLYERITKHEIKMKEDDLRLQQKQYANSNRMLGLDGILNIVIRKQWGDSYAE 847

Query: 851  ANGLLIRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSVTLDQSDDKLA 910
             +  L++H+QEQFK K+ KSES Y+A TDV ILRFM+E CW PMLAAFSV LDQSDD + 
Sbjct: 848  TSDDLMKHMQEQFKEKARKSESTYYAATDVVILRFMIEACWAPMLAAFSVPLDQSDDLIV 907

Query: 911  TSQCLLGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDAVKAIISIAIE 970
             + CL GF HA+H T++M ++T RDAFVTS+AKFT LH  AD+KQ+N++A+KAI+ +A E
Sbjct: 908  INICLEGFHHAIHATSLMSMKTHRDAFVTSLAKFTSLHSPADIKQRNIEAIKAILRLADE 967

Query: 971  DGNFLQEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDE-KTMKSVGLSSLKKK 1030
            +GN+LQ+AWEHI TC+SR E L LLGEGAPPDA+F  S   E+++ K  K   L  LK+K
Sbjct: 968  EGNYLQDAWEHILTCVSRFEQLHLLGEGAPPDATFFASKQNESEKSKQPKQYILPVLKRK 1027

Query: 1031 G-GLQNPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNFELNHVFAH 1090
            G G    A   V+R GSYDS SLG      V  EQ++ ++SNLNLL+Q+G  E+N VF+ 
Sbjct: 1028 GPGKSQYAATGVLR-GSYDSMSLGGKGSKNVRQEQMSSIVSNLNLLEQVG--EMNQVFSQ 1087

Query: 1091 SQSLNSEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLVWSRMWNVL 1150
            SQ LNSEAI+ FVKALCKVS+ EL+SP++PRVFSLTK+VE+AHYNMNRIRLVWS +W VL
Sbjct: 1088 SQKLNSEAIIDFVKALCKVSMDELRSPSNPRVFSLTKIVEIAHYNMNRIRLVWSSIWQVL 1147

Query: 1151 SDFFVSVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIVMQKSSSTE 1210
            S FFV++G SENLS+AIF MDSLRQL+MKFLEREELANYNFQNEF+ PFVIVM++S+  E
Sbjct: 1148 SGFFVTIGCSENLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMTPFVIVMRRSNDVE 1207

Query: 1211 IRELIVRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEKIVREYFPY 1270
            IRELI+RC+SQMVLSRVNNVKSGWKS+F+VFT AA D+ KNIV L+FE +EKI+REYFPY
Sbjct: 1208 IRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVFLSFEIIEKIIREYFPY 1267

Query: 1271 ITETETTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYEM-VVDDDS 1330
            ITETETTTFTDCV CL+ FTN+RF+ D+SL++IAFLR+CA KLAEG L           S
Sbjct: 1268 ITETETTTFTDCVNCLVAFTNNRFSKDISLSSIAFLRYCATKLAEGDLNSPSTNKYKGTS 1327

Query: 1331 SKAPDAPTSTDK-----------DDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNIL 1390
             K P +   + K           +++  +W PLL+GLS+L+ DPR  IRKS+L+++F+ L
Sbjct: 1328 GKIPQSSLHSGKSGKQENGEIVNNNHLYFWFPLLSGLSELSFDPRPEIRKSALQIMFDTL 1387

Query: 1391 KDHGHLFSRQFWVGVINTVVFPIFSSLYGKKEVDINESDEH-PEGST-----------WD 1450
            ++HGHLFS   W  V  +V+FPIF   Y +  +D +  DE   +GS+           W 
Sbjct: 1388 RNHGHLFSLPLWEKVFESVLFPIFD--YVRHSIDPSGEDESADQGSSGGEVDELDHDAWL 1447

Query: 1451 SDTCVVAAECLVDLFISFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELG 1510
             +TC +A + +VDLF+ F+  +   L  V+ +L  FI+ P    A  G++A +RL  +  
Sbjct: 1448 YETCTLALQLVVDLFVKFYTTVNPLLEKVLMLLVSFIKRPHQSLAGIGIAAFVRLMSDAD 1507

Query: 1511 SRLSGSEWREIFLALKEAATSTVPDFMKVLKTMDDVNVPGISQSSYDVDVASDQGLSTDG 1570
               S  +W E+  ALKEAA +T PDF   L           + +  + +  S    +TDG
Sbjct: 1508 GLFSEEKWLEVVSALKEAAKTTCPDFSYFLSEEYVARSQRSALNIQNSNAESAAPTATDG 1567

Query: 1571 LDDDDLQTASYI---VSRMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSIS 1630
             +++  +TA+++   +S  K    +QLL+IQ + ++Y  +    S  N  ++++    ++
Sbjct: 1568 -NEESQRTATHLYAAISDAKCRAAVQLLLIQAVMEIYNMYRPQLSAKNTLVLVDALHGVA 1627

Query: 1631 THAQKLNSEIILQKKLQKACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSL---TN 1690
             HA  +NS  IL+ +LQ+   + ++ DPP++  ENESYQ  L FLQN++ D +       
Sbjct: 1628 LHAHGINSNTILRSRLQELGPMTQMQDPPLLRLENESYQICLTFLQNLVADKTKKEEEEE 1687

Query: 1691 PTLVESELVAVCEQILRIYLKCTRT--QAERKETNNPVLHWILPLGSAKKEELAARTSLV 1713
               +ES LV +C+++L  Y++ + +  + + + +      W +PLGS K+ EL+AR  L+
Sbjct: 1688 EEEIESLLVNICQEVLNFYIETSSSAKKLQSESSRASEYRWRIPLGSGKRRELSARAPLI 1747

BLAST of MC04g0302 vs. TAIR 10
Match: AT1G01960.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 1596.3 bits (4132), Expect = 0.0e+00
Identity = 874/1776 (49.21%), Postives = 1201/1776 (67.62%), Query Frame = 0

Query: 11   SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDS-----------LGEAPLDPA- 70
            SR GR + P+LDK++KNA+WRKHS L   CK V+++L S            G +   P  
Sbjct: 8    SRLGRVVIPALDKVIKNASWRKHSKLAHECKSVIERLRSPENSSPVADSESGSSIPGPLH 67

Query: 71   -SGLAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANS 130
              G A  S  ++E +L PL+ A     +K+ +PA++C+ KL + G  RGE +        
Sbjct: 68   DGGAAEYSLAESEIILSPLINASSTGVLKIVDPAVDCIQKLIAHGYVRGEAD----PTGG 127

Query: 131  SPSTIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYL 190
              + ++ K++E++CK   L DEG+EL  L+ LL+AV S  + I GD L+ +VRTCY +YL
Sbjct: 128  PEALLLSKLIETICKCHELDDEGLELLVLKTLLTAVTSISLRIHGDSLLQIVRTCYGIYL 187

Query: 191  GGLTGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFC 250
            G     NQ  AK+ L Q+ V+VF R+E DS   P++ I V+EL+E  DK+ ++ ++    
Sbjct: 188  GSRNVVNQATAKASLVQMSVIVFRRMEADSSTVPIQPIVVAELMEPMDKSESDPSTTQSV 247

Query: 251  QNFVNEVMDASEGIAE----KKLY-----SFSSQLQNGNASPLKV--------------- 310
            Q F+ ++M   +G+      K  +     +F + L  G A+P  +               
Sbjct: 248  QGFITKIMQDIDGVFNSANAKGTFGGHDGAFETSLP-GTANPTDLLDSTDKDMLDAKYWE 307

Query: 311  ---------DNKGESDVGETE--DGAESGGCSKIREDGFHLFKNLCKLSMKFSSQEHPDD 370
                       KGE   GE E  D +E    +K+R D F +F+ LCKLSMK   +E P+ 
Sbjct: 308  ISMYKSALEGRKGELADGEVEKDDDSEVQIGNKLRRDAFLVFRALCKLSMKTPPKEDPE- 367

Query: 371  QILLRGKVLSLELLKVVMDNAGPVWHSVTVGFRFLNAIKQYLCLSLLKNSALSVMAIFQL 430
              L+RGK+++LELLK++++NAG V+ +     RFL AIKQYLCLSLLKNSA ++M IFQL
Sbjct: 368  --LMRGKIVALELLKILLENAGAVFRT---SDRFLGAIKQYLCLSLLKNSASNLMIIFQL 427

Query: 431  QCSIFTSLLTKFRSGLKAEMGIFFPMLVLRVLENVLQPSFLQKMTVLNLLDKISQDSQIM 490
             CSI  SL+++FR+GLKAE+G+FFPM+VLRVLENV QP F QKM VL  LDK+  DSQI+
Sbjct: 428  SCSILLSLVSRFRAGLKAEIGVFFPMIVLRVLENVAQPDFQQKMIVLRFLDKLCVDSQIL 487

Query: 491  VDIFVNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTALSPAQDITFRHESVKCLVSIIK 550
            VDIF+NYDCDV+S NIFER+VNGLLKTA G P G+ T L P Q+   + E++KCLV++++
Sbjct: 488  VDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGTVTTLLPPQEAAMKLEAMKCLVAVLR 547

Query: 551  SMGTWMDQQLKLDDTSLPKILE---NDASPESHSIISGEETAAVDSELNTDGNSE----S 610
            SMG W+++QL+L D    K+LE    +    SH + +G+          +D  SE    +
Sbjct: 548  SMGDWVNKQLRLPDPYSAKMLEIVDRNLEEGSHPVENGKGDGGHGGFERSDSQSELSSGN 607

Query: 611  SDTATLEQRRAYKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPEEVASFLKNTSGLNET 670
            SD   +EQRRAYK+ELQ+GIS+FN+KP +GIEFL+   KVG SPEE+A+FLK+ SGLN+T
Sbjct: 608  SDALAIEQRRAYKLELQEGISIFNQKPKKGIEFLIKANKVGDSPEEIAAFLKDASGLNKT 667

Query: 671  VIGDYLGEREEFPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLPGEAQKIDRIMEKFAE 730
            +IGDYLGERE+  LKVMHAYVDSF F+ M+F EAIR FLRGFRLPGEAQKIDRIMEKFAE
Sbjct: 668  LIGDYLGEREDLSLKVMHAYVDSFEFQGMEFDEAIRAFLRGFRLPGEAQKIDRIMEKFAE 727

Query: 731  RYCKCNPGSFARADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFIRNNRGIDDGKDLPDE 790
            R+CKCNP  F+ ADTAYVLAYSVI+LNTDAHN MVK KMT   FIRNNRGIDDGKDLP+E
Sbjct: 728  RFCKCNPKDFSSADTAYVLAYSVILLNTDAHNPMVKSKMTADGFIRNNRGIDDGKDLPEE 787

Query: 791  YLGALYDQIIRNEIKMNSDSSASQSKQATSINKLLGLDGILNLVSWKQTEEKAVGANGLL 850
            YL ALY++I RNEIKM  D    Q KQ T+ ++LLGLD ILN+V  ++ ++  +  +  L
Sbjct: 788  YLRALYERISRNEIKMKDDGLGPQQKQPTNSSRLLGLDTILNIVVPRRGDDMNMETSDDL 847

Query: 851  IRHIQEQFKAKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSVTLDQSDDKLATSQCL 910
            IRH+QE+FK K+ KSESVY+A +DV ILRFMVEVCW PMLAAFSV LDQSDD + T+ CL
Sbjct: 848  IRHMQERFKEKARKSESVYYAASDVIILRFMVEVCWAPMLAAFSVPLDQSDDAVITTLCL 907

Query: 911  LGFRHAVHVTAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDAVKAIISIAIEDGNFL 970
             GF HA+HVT++M L+T RDAFVTS+AKFT LH  AD+KQKN++A+KAI+ +A E+GN+L
Sbjct: 908  EGFHHAIHVTSVMSLKTHRDAFVTSLAKFTSLHSPADIKQKNIEAIKAIVKLAEEEGNYL 967

Query: 971  QEAWEHIFTCLSRIENLQLLGEGAPPDASFLTSSIIETDEKTM-KSVGLSSLKKKG-GLQ 1030
            Q+AWEHI TC+SR E+L LLGEGAPPDA+F      E+    + K   + ++K++  G  
Sbjct: 968  QDAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQTESGNSPLAKPNSVPAIKERAPGKL 1027

Query: 1031 NPAVMAVVRGGSYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNFELNHVFAHSQSLN 1090
              A  A++R GSYD + +   +   VT EQ+N+LISNLNLL+Q+G  +++ +F  SQ LN
Sbjct: 1028 QYAASAMIR-GSYDGSGVAGKASNTVTSEQMNNLISNLNLLEQVG--DMSRIFTRSQRLN 1087

Query: 1091 SEAIVAFVKALCKVSIAELQSPTDPRVFSLTKLVEVAHYNMNRIRLVWSRMWNVLSDFFV 1150
            SEAI+ FVKALCKVS+ EL+SP+DPRVFSLTK+VE+AHYNMNRIRLVWS +W+VLSDFFV
Sbjct: 1088 SEAIIDFVKALCKVSMDELRSPSDPRVFSLTKIVEIAHYNMNRIRLVWSSIWHVLSDFFV 1147

Query: 1151 SVGLSENLSVAIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIVMQKSSSTEIRELI 1210
            ++G S+NLS+AIF MDSLRQL+MKFLEREELANYNFQNEF++PFV+VM+KS + EIRELI
Sbjct: 1148 TIGCSDNLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMKPFVVVMRKSGAVEIRELI 1207

Query: 1211 VRCISQMVLSRVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEKIVREYFPYITETE 1270
            +RC+SQMVLSRV+NVKSGWKS+F++FT AA D  KNIV L+FE +EKI+R+YFP+ITETE
Sbjct: 1208 IRCVSQMVLSRVDNVKSGWKSMFMIFTTAAHDAHKNIVFLSFEMVEKIIRDYFPHITETE 1267

Query: 1271 TTTFTDCVRCLITFTNSRFNSDVSLNAIAFLRFCAVKLAE---GGLVCYEMVVDDDSSK- 1330
            TTTFTDCV CL+ FTN +F  D+SL AIAFL++CA KLAE   G  +     +     K 
Sbjct: 1268 TTTFTDCVNCLVAFTNCKFEKDISLQAIAFLQYCARKLAEGYVGSSLRRNPPLSPQGGKI 1327

Query: 1331 -APDAPTSTDKDDYASYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNILKDHGHLFSRQFW 1390
               D+    + D++   W PLLAGLS+L+ DPR+ IRK +L+VLF+ L++HG  FS   W
Sbjct: 1328 GKQDSGKFLESDEHLYSWFPLLAGLSELSFDPRAEIRKVALKVLFDTLRNHGDHFSLALW 1387

Query: 1391 VGVINTVVFPIFSSLYGKKEVDINESDE--------HPEGSTWDSDTCVVAAECLVDLFI 1450
              V  +V+F IF   Y +++VD +E D           +  +W  +TC +A + +VDLF+
Sbjct: 1388 ERVFESVLFRIFD--YVRQDVDPSEDDSTDQRGYNGEVDQESWLYETCSLALQLVVDLFV 1447

Query: 1451 SFFNVIRSQLPGVVAILTGFIRSPIHGPASTGVSALMRLAGELGSRLSGSEWREIFLALK 1510
            +F+  +   L  V+ +    I+ P    A  G++AL+RL  ++G + S  +W E+   +K
Sbjct: 1448 NFYKTVNPLLKKVLMLFVSLIKRPHQSLAGAGIAALVRLMRDVGHQFSNEQWLEVVSCIK 1507

Query: 1511 EAATSTVPDFMKVLK--TMDDVNVPGISQSSYDVDVASDQGLSTDGLDDDDLQTASYIVS 1570
            EAA +T PDF  V     M+DV+         + D  +D   S D L   + Q  + +V+
Sbjct: 1508 EAADATSPDFSYVTSEDLMEDVS---------NEDETNDN--SNDALRRRNRQLHA-VVT 1567

Query: 1571 RMKSHIGMQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQKLNSEIILQKKLQ 1630
              KS   +Q+ +IQ +TD+Y  +    + +++ ++ +    I ++A K+N++++L+ KLQ
Sbjct: 1568 DAKSKASIQIFVIQAVTDIYDMYRMSLTANHMLMLFDAMHGIGSNAHKINADLLLRSKLQ 1627

Query: 1631 KACSILEISDPPVVHFENESYQSYLNFLQNMLMDTSSLTNPTLVESELVAVCEQILRIY- 1690
            +  S LE  + P++  ENES+Q+ + FL N++ D     N   +ES L+++C ++L  Y 
Sbjct: 1628 ELGSSLESQEAPLLRLENESFQTCMTFLDNLISDQPVGYNEAEIESHLISLCREVLEFYI 1687

Query: 1691 -LKCTRTQAERKETNNPVLHWILPLGSAKKEELAARTSLVVSALRVLSGLQRDLFKRYVS 1713
             + C++ Q+ R         W +P GS KK+EL AR  LVV+A++ L  +   LFK+ + 
Sbjct: 1688 NISCSKEQSSR---------WAVPSGSGKKKELTARAPLVVAAIQTLGNMGESLFKKNLP 1746

BLAST of MC04g0302 vs. TAIR 10
Match: AT3G43300.1 (HOPM interactor 7 )

HSP 1 Score: 801.2 bits (2068), Expect = 1.6e-231
Identity = 583/1793 (32.52%), Postives = 902/1793 (50.31%), Query Frame = 0

Query: 64   LSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPSTIV 123
            L   + E VL+PL LA +   +K+ + AL+CL KL +     G+    +   NS+P T +
Sbjct: 99   LGGAEVELVLKPLRLAFETKNLKIFDAALDCLHKLIAYDHLEGD-PGLDGGKNSAPFTDI 158

Query: 124  YKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGLTGT 183
              +V S C      D  + L  L+VLL+AV S    + G+ L+ V+R CYN+ L   +  
Sbjct: 159  LNMVCS-CVDNSSPDSTV-LQVLKVLLTAVASGKFKVHGEPLLGVIRVCYNIALNSKSPI 218

Query: 184  NQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNFVNE 243
            NQ  +K++L Q++ +VF R+E D + A   +     +   T    NE            E
Sbjct: 219  NQATSKAMLTQMISIVFRRMETDIVSASSTVSQEEHVSGDTSSPKNE------------E 278

Query: 244  VMDASEGIAEKKLYSFSSQLQNGNASPLK--------VDNKG----------ESDVGETE 303
            +  A E   E  L    +Q ++   + ++         D KG            D  + +
Sbjct: 279  ITAADENEKEMTLGDALTQAKDTTLASVEELHTLVGGADIKGLEAALDKAVHLEDGKKIK 338

Query: 304  DGAESGGCSKIREDGFHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGP 363
             G E    S  + D   +F+ LCK+ MK  S     D++  + ++LSLELL+ +++    
Sbjct: 339  RGIELESMSIGQRDALLVFRTLCKMGMKEDS-----DEVTTKTRILSLELLQGMLEG--- 398

Query: 364  VWHSVTVGFRFLNAIKQYLCLSLLKNSALSVMAIFQLQCSIFTSLLTKFRSGLK------ 423
            V HS T  F F++++K YL  +LL+ S      IFQ    IF+ LL +FR  LK      
Sbjct: 399  VSHSFTKNFHFIDSVKAYLSYALLRASVSQSSVIFQYASGIFSVLLLRFRDSLKVSMDCY 458

Query: 424  -------------AEMGIFFPMLVLRVLENVLQPSFLQKMTVLNLLDKISQDSQIMVDIF 483
                          E+GIFFP++VLR L+N   P+  QKM VL +L+K+ +D Q++VD++
Sbjct: 459  LSPYFSDPKSHSQGEIGIFFPIIVLRSLDNSECPND-QKMGVLRMLEKVCKDPQMLVDVY 518

Query: 484  VNYDCDVDSPNIFERIVNGLLKTALGPPSGSTTALSPAQDITFRHESVKCLVSIIKSMGT 543
            VNYDCD+++PN+FER+V  L K A G  S        +Q  + +  S++CLV+++KS+  
Sbjct: 519  VNYDCDLEAPNLFERMVTTLSKIAQGSQSADPNPAMASQTASVKGSSLQCLVNVLKSLVD 578

Query: 544  WMDQQLKLDDTSLPKILENDASPESHSIISGEETAAVDSELNTDGNSESSDTATLEQRRA 603
            W  ++++ +              E+ +  + E++A+    + T   S     +  E+ +A
Sbjct: 579  W--EKIRRE-------------AENSTRNANEDSASTGEPIET--KSREDVPSNFEKAKA 638

Query: 604  YKIELQKGISLFNRKPSRGIEFLMSTKKVGGSPEEVASFLKNTSGLNETVIGDYLGEREE 663
            +K  ++  IS FNR   +G+E+L++ K V  +P  VA FL++TS L++ +IGDYLG+ EE
Sbjct: 639  HKSTMEAAISEFNRNSVKGVEYLIANKLVERNPASVAQFLRSTSSLSKVMIGDYLGQHEE 698

Query: 664  FPLKVMHAYVDSFNFKAMDFGEAIRFFLRGFRLPGEAQKIDRIMEKFAERYCKCNPGSFA 723
            FPL VMHAYVDS  F  M F  AIR FL+GFRLPGEAQKIDRIMEKFAERYC  NPG F 
Sbjct: 699  FPLAVMHAYVDSMKFSEMKFHSAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFK 758

Query: 724  RADTAYVLAYSVIMLNTDAHNNMVKDKMTKADFIRNNRGIDDGKDLPDEYLGALYDQIIR 783
             ADTAYVLAY+VIMLNTDAHN MV  KM+K+DF R N   D     P E L  +YD I++
Sbjct: 759  NADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNATNDPEDCAPTELLEEIYDSIVQ 818

Query: 784  NEIKMNSDSSASQ-SKQATSINKLLGLDGILNLVSWKQ-TEEKAVGANGLLIRHIQEQFK 843
             EIK+  D +  + S Q     +  GL  ILNL   K+ +   A      ++R  QE F+
Sbjct: 819  EEIKLKDDDTMKKLSSQRPGGEERGGLVSILNLGLPKRISAADAKSETEDIVRKTQEIFR 878

Query: 844  AKSGKSESVYHAVTDVTILRFMVEVCWGPMLAAFSVTLDQSDDKLATSQCLLGFRHAVHV 903
             K G    V+H V  V I+R MVE    P+LAAFSVT++  D+K     C+ GF+  +H+
Sbjct: 879  -KHGVKRGVFHTVEQVDIIRPMVEAVGWPLLAAFSVTMEVGDNKPRILLCMEGFKAGIHI 938

Query: 904  TAMMGLQTQRDAFVTSMAKFTYLHCAADMKQKNVDAVKAIISIAIEDGNFLQEAWEHIFT 963
              ++G+ T R AF+TS+ +FT+LH   +M+ KNV+A++ ++ +   + + LQ+ W  +  
Sbjct: 939  AYVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRILLGLCDSEPDTLQDTWNAVLE 998

Query: 964  CLSRIENLQLLGEGAPPDASFLTSSIIETDEKTMKSVGLSSLKKKGGLQNPAVMAVVRGG 1023
            C+SR+E +                                       +  P + A V  G
Sbjct: 999  CVSRLEFI---------------------------------------ISTPGIAATVMHG 1058

Query: 1024 SYDSTSLGVNSPGPVTPEQINHLISNLNLLDQIGNFELNHVFAHSQSLNSEAIVAFVKAL 1083
            S   +  GV                 +  L ++       VF +S  L SE++V F  AL
Sbjct: 1059 SNQISRDGV-----------------VQSLKELAGRPAEQVFVNSVKLPSESVVEFFTAL 1118

Query: 1084 CKVSIAEL-QSPTDPRVFSLTKLVEVAHYNMNRIRLVWSRMWNVLSDFFVSVGLSENLSV 1143
            C VS  EL QSP   RVFSL KLVE+++YN+ RIR+VW+R+W+VL++ FVS G   +  +
Sbjct: 1119 CGVSAEELKQSPA--RVFSLQKLVEISYYNIARIRMVWARIWSVLAEHFVSAGSHHDEKI 1178

Query: 1144 AIFVMDSLRQLAMKFLEREELANYNFQNEFLRPFVIVMQKSSSTEIRELIVRCISQMVLS 1203
            A++ +DSLRQL MK+LER EL N+ FQN+ L+PFVI+M+ + S  IR LIV CI QM+ S
Sbjct: 1179 AMYAIDSLRQLGMKYLERAELTNFTFQNDILKPFVIIMRNTQSQTIRSLIVDCIVQMIKS 1238

Query: 1204 RVNNVKSGWKSVFLVFTAAAADERKNIVLLAFETMEKIVREYFPYITETETTTFTDCVRC 1263
            +V ++KSGW+SVF++FTAAA DE ++IV  +FE +E+++ E+F  +       F DCV C
Sbjct: 1239 KVGSIKSGWRSVFMIFTAAADDEVESIVEKSFENVEQVILEHFDQVI---GDCFMDCVNC 1298

Query: 1264 LITFTNSRFNSDVSLNAIAFLRFCAVKLAEGGLVCYEMVVDDDSSKAPDAPTSTDKDDYA 1323
            LI F N++ +  +SL AIA LR C  +LAEG       ++     K  D       D   
Sbjct: 1299 LIRFANNKASDRISLKAIALLRICEDRLAEG-------LIPGGVLKPVDGNEDETFDVTE 1358

Query: 1324 SYWVPLLAGLSKLTSDPRSPIRKSSLEVLFNILKDHGHLFSRQFWVGVINTVVFPIFSSL 1383
             YW P+LAGLS LTSD R  +R  +LEVLF++L + G+ FS  FW  + + ++FPIF  +
Sbjct: 1359 HYWFPMLAGLSDLTSDYRPEVRNCALEVLFDLLNERGNKFSTPFWESIFHRILFPIFDHV 1418

Query: 1384 -YGKKEVDINESDEHPEGSTWDSDTCVVAAECLVDLFISFFNVIRSQLPGVVAILTGFIR 1443
             +  KE  I+       G     +T + + + L +LF +F+  +   LP ++++L    +
Sbjct: 1419 SHAGKESLIS------SGDVKFRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAK 1478

Query: 1444 SPIHGPASTGVSALMRLAGELGSRLSGSEWREIFLALKEAATSTVP-------------- 1503
                   S  + AL+ L    G + S  +W  +  ++++A+ +T P              
Sbjct: 1479 KSDQTVVSISLGALVHLIEVGGHQFSEGDWDMLLKSIRDASYTTQPLELLNALSFDNPKK 1538

Query: 1504 -------------DFMKVLKTMDDVNVPG--ISQSSYDVDV------------------- 1563
                         D  +V +  DD+   G   +Q+S  +                     
Sbjct: 1539 NLVLAGDIEADASDSPRVDRNPDDIKDNGKVSAQASPRIGTHGTSLESGIPPKADGSEGR 1598

Query: 1564 ASDQGLSTDGLDDDDLQTAS-------------YIVSRMKSHIG---------------- 1623
             S  G +   +DD +LQ +               + S+ KS +                 
Sbjct: 1599 PSSSGRAQKDVDDVNLQRSQTFGQRFMDNLFLRNLTSQPKSSVAEVTVPSSPYKHEDPTE 1658

Query: 1624 -------------------MQLLIIQVITDLYKNHAQPFSEDNISIILEIFSSISTHAQK 1683
                                QLL++  I  + + +           I++I  S    A  
Sbjct: 1659 PDSREEESPALGAIRGKCITQLLLLGAINSIQQKYWSNLKTPQKIAIMDILFSFIEFASS 1718

Query: 1684 LNSEIILQKKLQKACSILEISDPP--VVHFENESYQSYLNFLQNM---LMDTSSLTNPTL 1712
             NS   L+ ++      +    PP  ++  E E    YL+ LQ     L D +S +   L
Sbjct: 1719 YNSYSNLRTRMNH----IPTERPPLNLLRQELEGTTIYLDVLQKTTSGLADDASNSEDRL 1756

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JSZ50.0e+0068.75Brefeldin A-inhibited guanine nucleotide-exchange protein 1 OS=Arabidopsis thali... [more]
F4JN050.0e+0064.19Brefeldin A-inhibited guanine nucleotide-exchange protein 4 OS=Arabidopsis thali... [more]
Q9LZX80.0e+0048.83Brefeldin A-inhibited guanine nucleotide-exchange protein 2 OS=Arabidopsis thali... [more]
Q9LPC50.0e+0049.21Brefeldin A-inhibited guanine nucleotide-exchange protein 3 OS=Arabidopsis thali... [more]
F4IXW27.6e-23432.86Brefeldin A-inhibited guanine nucleotide-exchange protein 5 OS=Arabidopsis thali... [more]
Match NameE-valueIdentityDescription
XP_022136256.10.099.59brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 [Momordic... [more]
XP_038888749.10.090.68brefeldin A-inhibited guanine nucleotide-exchange protein 1 [Benincasa hispida][more]
XP_008451186.10.090.03PREDICTED: brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X... [more]
XP_004153755.10.089.76brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 [Cucumis ... [more]
KAA0059594.10.089.97brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 [Cucumis ... [more]
Match NameE-valueIdentityDescription
A0A6J1C3090.099.59brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 OS=Momord... [more]
A0A0A0K1N60.089.76SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G027890 PE=4 S... [more]
A0A1S3BQB30.090.03brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 OS=Cucumi... [more]
A0A5A7UWX00.089.97Brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 OS=Cucumi... [more]
A0A6J1JLY00.089.50brefeldin A-inhibited guanine nucleotide-exchange protein 1-like OS=Cucurbita ma... [more]
Match NameE-valueIdentityDescription
AT4G38200.10.0e+0068.75SEC7-like guanine nucleotide exchange family protein [more]
AT4G35380.10.0e+0064.19SEC7-like guanine nucleotide exchange family protein [more]
AT3G60860.10.0e+0048.83SEC7-like guanine nucleotide exchange family protein [more]
AT1G01960.10.0e+0049.21SEC7-like guanine nucleotide exchange family protein [more]
AT3G43300.11.6e-23132.52HOPM interactor 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000904Sec7 domainSMARTSM00222sec7_5coord: 564..749
e-value: 1.1E-96
score: 337.2
IPR000904Sec7 domainPFAMPF01369Sec7coord: 568..749
e-value: 8.7E-70
score: 234.1
IPR000904Sec7 domainPROSITEPS50190SEC7coord: 560..747
score: 45.469788
IPR000904Sec7 domainCDDcd00171Sec7coord: 567..749
e-value: 5.42297E-86
score: 276.796
IPR023394Sec7, C-terminal domain superfamilyGENE3D1.10.1000.11coord: 642..762
e-value: 3.1E-48
score: 164.6
IPR032817Mon2, C-terminalPFAMPF16206Mon2_Ccoord: 1266..1341
e-value: 1.0E-6
score: 27.3
NoneNo IPR availableGENE3D1.10.220.20coord: 551..641
e-value: 2.3E-25
score: 90.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..290
NoneNo IPR availablePANTHERPTHR10663GUANYL-NUCLEOTIDE EXCHANGE FACTORcoord: 9..1706
NoneNo IPR availablePANTHERPTHR10663:SF356BREFELDIN A-INHIBITED GUANINE NUCLEOTIDE-EXCHANGE PROTEIN 4coord: 9..1706
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 1107..1325
e-value: 2.3E-6
score: 29.7
IPR015403Sec7, C-terminalPFAMPF09324DUF1981coord: 1117..1199
e-value: 1.8E-29
score: 101.5
IPR032691Guanine nucleotide exchange factor, N-terminalPFAMPF12783Sec7_Ncoord: 298..458
e-value: 1.4E-35
score: 122.5
IPR032629Mon2, dimerisation and cyclophilin-binding domainPFAMPF16213DCBcoord: 17..206
e-value: 3.5E-21
score: 75.7
IPR035999Sec7 domain superfamilySUPERFAMILY48425Sec7 domaincoord: 564..755
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 394..1706

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0302.1MC04g0302.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
biological_process GO:0032012 regulation of ARF protein signal transduction
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0005829 cytosol
cellular_component GO:0016020 membrane
cellular_component GO:0005802 trans-Golgi network
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity