MC04g0273 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0273
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionThreonyl-tRNA synthetase
LocationMC04: 2166163 .. 2171525 (+)
RNA-Seq ExpressionMC04g0273
SyntenyMC04g0273
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAACGAGATGAAAAATCGAGGAAAACGATAAAGTGACCCTCATGAATCCTTAACAAAATCGTGCAGAAAAACAACCCCAATAAGAACATTAATACCTTCTCTTCCCGCTTTTGCTCACGAGCCTCGGGATTCCGCTCAATGAAAATGAGGAGCTCACGCGTGTACCGCACGAGCTCCACCGGCGGTCGTTGCTTCGGCTTGAAAAGTCCCTTCATCTCTATTCGGGTTTCGATGAATGCAACTCTTCCTCCTCAAAATTTTTTTCTTCTCGAGAAAAAAAGAAGGGAGATGAGAAAATGGGAAGAGGAGAAAGGGGCGAAGGCGTAGTTGTGTACCGGTTTTGTGGGGGCGGAATTGAAGTAATGAGGCGTTGGTTACAAATATAGAGGAAAAAGGAATCGACTTTTACAGCGGTGGCGCGTGTACTTTTGTTCTCTAATCACAACCGGTAATTCAATCCTGAAATTGGAAAAGACCCACTTAAAATATGCGCCATTAATCGTAGTCCATGATTAAAAATTAAAAATTTATGCGTTTATCAAGATTCATTAAAGGGATACTTTAGTATCATATAAATTCAATTTTCGATCAGAAAAATATCCAAAGTTCCACCACACACAGCATCAGAGGAGAGGAGAGTCATATCTTCTACTCTCTCTTTTCCTCTCGCGCCTTCAAATGTTTATACTTCAAAGAATGGCGAGTTCCACTGGGTTACTTCTAAATCCGCCTCTCGTAAGACCCATTTCGTCGAATGTTTCTCCACATCGTTTCTTCCTTGCAGTTGCAACCCAATTTGCAGGAAATAGGGACAAATCTCTTTTCCATAGCAAGAAGGCAAATGGGTTTTCGAGTTCCGTTGCAGTGGCTACTGATTCCTCAACGACGTCAGTTTCGACCCAGAACGAGGATTTGGTGGAGACCCAGAAGGAGGAAAGCGAGAAGGTCGTTCTTCCAACTAATGAATCGTCGGAGAAGCTTCTCAGAATTCGTCACACGGTAATAGTTAACATACTGGTTGCCATGAACTTGTATTGTAATCTATTTTTTCTGTGATTTCTCATTATGGAGTTTCAGCTGATTATTCTTTGAGTAAGGTTTTAGTTTCTTATCTGGGAATTTGAGGAATTCTGGTGCTTAAACAAGGTTCTTAACGTGGTTTTCCTAAGAAATTTAGGTTTGAACTAGGGAGGAGTTTCATAATTTAAACAGGGTGTTGCTAATCTAACAGGGTTTTCTGAATTAGAATTTGTATCTTAGAGGTAAGCATCAATGTGAAAATTTTCTGGGATTTATGAATCGTGCTTTTGAAATTATCTTCATGAAGGACCTATTTTTATGCCTTCGTTTGAACTGCTTGATATATTTATACAATTGCAGTGTGCCCATGTGATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGGCCATGGATAGAAAATGGGTTTTACTATGATTTTGATATGGAGCCCTTGGGAGATAAAGATTTGAAGAAAATCAAGAAGGAGATGGTTGGTATACAAAACCTTTGAGCACTGAAGTCACTGGACTTTTTCTTCATAATTTAACATCACAAGGTTTGGAATCTATGAATTAGTGTATGCTTTCCGGGTATGGAATTTATTTCTTATATTATTTGTAGGAACGCATTATTAGCAGAAACTTACCACTTCTAAGAGAAGAAGTTTCAAGGGATGAAGCTCAGAGAAGAATTATGGCAATCAACGAACCTTACAAGATGGAGATTCTGGAAAGCATAAAGGAAGATCCCATAACCATCTATCATATTGGTAGGGATTGCAACCATCTTTGTAATTATTTATTGCAACTGTTGAGATTCCTTTGTATTTAATGCTTAACAACTTTAGATATTCTTCTTGAATTGAAGGTTTGACCATTTTATCTTCTTTGTAATTGCAATTTGGCATATGAATACCTTGAGTTTTGACTCTTTTTCTTTTTGTTTCCAGACGGCTATATTTTTTGTTCTATAAATAGGATTTTCTATTAAATTATATCTCAAGTTTTTCAATCAATAGAACATTTTCACTTCAATGTTTTTCTCTCAGTTTGCTGTTGTTTCTGGTTCTGCAACTCTTATTCCTCTTATTCTTCTTATTCCTTTTTTCCCCTTCTAGAATGATGTTAGAAGTTTGTGCATTTGGCTGAAACTTAAAATGGGAGCAAGATTGGGACTTTTCAAGCTAAATATTAAAAACAGTATACTCCAACCAGTTTTACAAGTTTCATATTCATCACCAAATTTTCGTTCTTCTATACACCATAAAAAGGAAGAGCAGGATTGTGTTGGAGATGACTAGATTATCATTATTATTATTTTTTTGAAAAGATGTTAAAGATTACTTTTTCATTTTTGGGGTAGATGGTATATTTATTGTTCTTTACTTGGTTAACAGACCTCTTACGTGGGCTGTTGGAGTCAAAAGTGCTTATGAAGCTTGAACAAATATAAAATTTTCTTGTAAACACTTTGAGGGTTTTTTTTTTATCTATTTGTTATTTATCTTTTTTGTTTTCCTTATGTGGAGAGATTCAAACATGTCAAGAAGGGTGTCCAAGGGATTTGTCCATATTCCTCATTCAAAATGCTATAGATTAATTTATTTGAAGACTAGTAAATTAAGGTAGATCAACTATCTAGTAAATTAAGAACATCCTGTGGTGAGCTTGGTCTATTTTGATCAGCTGCTATTCCCATCCCTTAATTTCATTTGAAGATTAAATATGTAATGCTCTCTTCAGACTTTAATTGGGATTAGGTTGAAGCTTAAAATTTTCCTAATATCTTCATTCATTGTAATGCCCTGTATTCTCCACAGGAAATGAGTGGTGGGACCTTTGTGCTGGCCCTCATGTTGAATCTACTGGTAAAATCAACAAAAGAGCTGTTGAGCTTGAAACCGTTGCCGGTGCATACTGGAGAGGAGATGAGAAGAATCCAATGCTACAGAGGATCTATGGCACTGCATGGGAGACGGAAGAACAATTAAAAGCATACCTTCACTTCAAAGAGGAGGCTAAACGACGCGATCACCGACGCCTTGGGAAAGATCTTGATCTATTTTCCATTCAGGTTTAGATTGCCTCGACTTCTGATTGTTTGCAAAAGTTATGCTCTTAGAATTCGGTGGTCTGAATTATTTTAGAATATTGGCTGAACATAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCATCCAAAGGGTGCCATCGTGAGGAGCATAATTGAAGATTTGTGGAAGAAATTGCACAGGCAACGCGGTTATGATCTGCTCTATACCCCACATGTGGCGAAGGCAGAGCTTTGGAAAACCAGTGGTCATTCAGACTTCTATGGAGAGAATATGTACAATCAGATGGACGTTGAGGATGAGTTATATCAACTTCGACCTATGAATTGCCCCTACCACATTCTATTTTACAAAAGGAGGCTGACCTCATATCAAGATTTTCCTATTCGGGTCGCGGAATTGGGGACGGTTTATAGATATGAGTTGTCTGGTAGCTTGCACGGGCTTTTCCGTGTCAGAGGTTTTACCCAGGTATACATTATGTTGAATGCTGTCGCTCTTTTGCATGTTGAAATTTTACAAGTTGTGATCTGCTAGTTCTGCTCTATGCAAAAGTCGAAATTGACTCGATGTGGCTTTTGCAGGATGATGCTCACATATTTTGTCTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATCTAACAGAAGAATTATTACTTCAATTTGGCTTTAACAAATTTGAAGTCAATCTCTCTACTAGACCACCAAAATCAGTGGGGAATGATGAGATCTGGGAGAAAGCAACATCTGCTCTTAGAGATGCTCTAAGTGATAAGGGGTGGAGCTATCAAATTGATGAAGGTGGTGGAGCCTTTTACGGACCGAAAATCGATATTAAGATCGAGGATGCATTGGGAAGGAAGTGGCAATGCTCAACTATTCAGGCAATGCCTCAACTTAGTATTGCTTTTTATTGGTAGATTCAAGCAGGATTTCTCTCTCTCTATCCTAAAATCTTCTGAATTTGTACAAACTGAAGTTCAAGGCAGTTTCTATTGATAAAATGGCAAACTATCGACTACCATAGGTTGACCCAGTGGCCAATAAAGACCAATATAAATAATAAAAGGCAGAGAGAATGTGTCATCTTCTTAGAATTTAATATCTTATGAGTTACTTTTGCAATCAAATAAATGCTAGGGTCAGGCGGTTGTCCTATGAAAATAGTGGAAGTGTGCGTAAGTTGGTTCAAATAAACAAATATAGGAAACAAAAAAGCAAAGCTATCTTACTTGAGTTTGGTCATGCAAATATGTTTTTTTTCCTTAATATTTAAGTTTTGGTTTTTCAGGTTGATTTTAATCTACCTCAGCGTTTCGACATCACATACGTTAATTCAAACTCGGAAAAGAAGAGACCTATCATGATTCACCGAGCGGTGCTAGGATCCTTGGAGCGATTCTTCGGTATTCTAATTGAAAACTATGCAGGGGATTTTCCACTATGGCTTTCTCCCGTCCAAGTCCGAGTTTTGCCCGTGACGGACTCCCAGGTGATTAGCTATATGTATAAGAATACAACTACCAACGGTTCTTGATTACATTAGTAATCTTGTAGGACTTGTTTGGACTGTCACGAAGAATTTGCATGATCTTGTCTTTATCTTCTATGAAAGTTCATGTCTATGGTGGCATGGCGAGGGTCACTCGGATGGACGGGACCTCGTAAATTTGATTTACTAACTGAACCATGTCTATAGATTCATCTTAGGGATTAAAGTTGTAATCTCTCACTGAAATCTCCATTGTCCATTGTATGTTTATCCCAGCTCCCCTACTGCAAAGAGGTGACCGAAAAACTCAAAGCGGTTGGTGTTCGAGCAGAATTTTGTCACGGGGAGCGGCTGCCTAAGCTTATTAGAAATGCAGAGACCCAAAAGATACCGTTAATGGCGGTTGTTGGCGCCAAGGAAGTCGAGACACAAACTGTGACAGTGAGATCCAGGTTTGGTGGGGAGATAGGGACCATAACAGTTGATGATTTTGTTGGAAAAATCAAGTTTGCCTCGGAGAATAGAACATCATTTTGAAGTTCTTTGGTGGGATGAATTTTGACCACTGTATTTTACTTTTATTTAACTTGAGATTGCCTCATGGAGGTTAATTCTCAGTGTACTTTTTGAATAATTTTTCACTCCATATTTTTACTGTAAATGTCAATGTAATCCTGTAGTTAGAGGAGTTTTCTTTTCCTTTGAAGGGAATAATAAGTAAAGATATACTTCTTATTTTCTTTTTCACGTCA

mRNA sequence

GAAAACGAGATGAAAAATCGAGGAAAACGATAAAGTGACCCTCATGAATCCTTAACAAAATCGTGCAGAAAAACAACCCCAATAAGAACATTAATACCTTCTCTTCCCGCTTTTGCTCACGAGCCTCGGGATTCCGCTCAATGAAAATGAGGAGCTCACGCGTGTACCGCACGAGCTCCACCGGCGGTCGTTGCTTCGGCTTGAAAAGTCCCTTCATCTCTATTCGGGTTTCGATGAATGCAACTCTTCCTCCTCAAAATTTTTTTCTTCTCGAGAAAAAAAGAAGGGAGATGAGAAAATGGGAAGAGGAGAAAGGGGCGAAGGCGTAGTTGTGTACCGGTTTTGTGGGGGCGGAATTGAAGTAATGAGGCGTTGGTTACAAATATAGAGGAAAAAGGAATCGACTTTTACAGCGGTGGCGCGTGTACTTTTGTTCTCTAATCACAACCGGTAATTCAATCCTGAAATTGGAAAAGACCCACTTAAAATATGCGCCATTAATCGTAGTCCATGATTAAAAATTAAAAATTTATGCGTTTATCAAGATTCATTAAAGGGATACTTTAGTATCATATAAATTCAATTTTCGATCAGAAAAATATCCAAAGTTCCACCACACACAGCATCAGAGGAGAGGAGAGTCATATCTTCTACTCTCTCTTTTCCTCTCGCGCCTTCAAATGTTTATACTTCAAAGAATGGCGAGTTCCACTGGGTTACTTCTAAATCCGCCTCTCGTAAGACCCATTTCGTCGAATGTTTCTCCACATCGTTTCTTCCTTGCAGTTGCAACCCAATTTGCAGGAAATAGGGACAAATCTCTTTTCCATAGCAAGAAGGCAAATGGGTTTTCGAGTTCCGTTGCAGTGGCTACTGATTCCTCAACGACGTCAGTTTCGACCCAGAACGAGGATTTGGTGGAGACCCAGAAGGAGGAAAGCGAGAAGGTCGTTCTTCCAACTAATGAATCGTCGGAGAAGCTTCTCAGAATTCGTCACACGTGTGCCCATGTGATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGGCCATGGATAGAAAATGGGTTTTACTATGATTTTGATATGGAGCCCTTGGGAGATAAAGATTTGAAGAAAATCAAGAAGGAGATGGAACGCATTATTAGCAGAAACTTACCACTTCTAAGAGAAGAAGTTTCAAGGGATGAAGCTCAGAGAAGAATTATGGCAATCAACGAACCTTACAAGATGGAGATTCTGGAAAGCATAAAGGAAGATCCCATAACCATCTATCATATTGGAAATGAGTGGTGGGACCTTTGTGCTGGCCCTCATGTTGAATCTACTGGTAAAATCAACAAAAGAGCTGTTGAGCTTGAAACCGTTGCCGGTGCATACTGGAGAGGAGATGAGAAGAATCCAATGCTACAGAGGATCTATGGCACTGCATGGGAGACGGAAGAACAATTAAAAGCATACCTTCACTTCAAAGAGGAGGCTAAACGACGCGATCACCGACGCCTTGGGAAAGATCTTGATCTATTTTCCATTCAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCATCCAAAGGGTGCCATCGTGAGGAGCATAATTGAAGATTTGTGGAAGAAATTGCACAGGCAACGCGGTTATGATCTGCTCTATACCCCACATGTGGCGAAGGCAGAGCTTTGGAAAACCAGTGGTCATTCAGACTTCTATGGAGAGAATATGTACAATCAGATGGACGTTGAGGATGAGTTATATCAACTTCGACCTATGAATTGCCCCTACCACATTCTATTTTACAAAAGGAGGCTGACCTCATATCAAGATTTTCCTATTCGGGTCGCGGAATTGGGGACGGTTTATAGATATGAGTTGTCTGGTAGCTTGCACGGGCTTTTCCGTGTCAGAGGTTTTACCCAGGATGATGCTCACATATTTTGTCTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATCTAACAGAAGAATTATTACTTCAATTTGGCTTTAACAAATTTGAAGTCAATCTCTCTACTAGACCACCAAAATCAGTGGGGAATGATGAGATCTGGGAGAAAGCAACATCTGCTCTTAGAGATGCTCTAAGTGATAAGGGGTGGAGCTATCAAATTGATGAAGGTGGTGGAGCCTTTTACGGACCGAAAATCGATATTAAGATCGAGGATGCATTGGGAAGGAAGTGGCAATGCTCAACTATTCAGGCAATGCCTCAACTTATTTTGGTTTTTCAGGTTGATTTTAATCTACCTCAGCGTTTCGACATCACATACGTTAATTCAAACTCGGAAAAGAAGAGACCTATCATGATTCACCGAGCGGTGCTAGGATCCTTGGAGCGATTCTTCGGTATTCTAATTGAAAACTATGCAGGGGATTTTCCACTATGGCTTTCTCCCGTCCAAGTCCGAGTTTTGCCCGTGACGGACTCCCAGCTCCCCTACTGCAAAGAGGTGACCGAAAAACTCAAAGCGGTTGGTGTTCGAGCAGAATTTTGTCACGGGGAGCGGCTGCCTAAGCTTATTAGAAATGCAGAGACCCAAAAGATACCGTTAATGGCGGTTGTTGGCGCCAAGGAAGTCGAGACACAAACTGTGACAGTGAGATCCAGGTTTGGTGGGGAGATAGGGACCATAACAGTTGATGATTTTGTTGGAAAAATCAAGTTTGCCTCGGAGAATAGAACATCATTTTGAAGTTCTTTGGTGGGATGAATTTTGACCACTGTATTTTACTTTTATTTAACTTGAGATTGCCTCATGGAGGTTAATTCTCAGTGTACTTTTTGAATAATTTTTCACTCCATATTTTTACTGTAAATGTCAATGTAATCCTGTAGTTAGAGGAGTTTTCTTTTCCTTTGAAGGGAATAATAAGTAAAGATATACTTCTTATTTTCTTTTTCACGTCA

Coding sequence (CDS)

ATGTTTATACTTCAAAGAATGGCGAGTTCCACTGGGTTACTTCTAAATCCGCCTCTCGTAAGACCCATTTCGTCGAATGTTTCTCCACATCGTTTCTTCCTTGCAGTTGCAACCCAATTTGCAGGAAATAGGGACAAATCTCTTTTCCATAGCAAGAAGGCAAATGGGTTTTCGAGTTCCGTTGCAGTGGCTACTGATTCCTCAACGACGTCAGTTTCGACCCAGAACGAGGATTTGGTGGAGACCCAGAAGGAGGAAAGCGAGAAGGTCGTTCTTCCAACTAATGAATCGTCGGAGAAGCTTCTCAGAATTCGTCACACGTGTGCCCATGTGATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGGCCATGGATAGAAAATGGGTTTTACTATGATTTTGATATGGAGCCCTTGGGAGATAAAGATTTGAAGAAAATCAAGAAGGAGATGGAACGCATTATTAGCAGAAACTTACCACTTCTAAGAGAAGAAGTTTCAAGGGATGAAGCTCAGAGAAGAATTATGGCAATCAACGAACCTTACAAGATGGAGATTCTGGAAAGCATAAAGGAAGATCCCATAACCATCTATCATATTGGAAATGAGTGGTGGGACCTTTGTGCTGGCCCTCATGTTGAATCTACTGGTAAAATCAACAAAAGAGCTGTTGAGCTTGAAACCGTTGCCGGTGCATACTGGAGAGGAGATGAGAAGAATCCAATGCTACAGAGGATCTATGGCACTGCATGGGAGACGGAAGAACAATTAAAAGCATACCTTCACTTCAAAGAGGAGGCTAAACGACGCGATCACCGACGCCTTGGGAAAGATCTTGATCTATTTTCCATTCAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCATCCAAAGGGTGCCATCGTGAGGAGCATAATTGAAGATTTGTGGAAGAAATTGCACAGGCAACGCGGTTATGATCTGCTCTATACCCCACATGTGGCGAAGGCAGAGCTTTGGAAAACCAGTGGTCATTCAGACTTCTATGGAGAGAATATGTACAATCAGATGGACGTTGAGGATGAGTTATATCAACTTCGACCTATGAATTGCCCCTACCACATTCTATTTTACAAAAGGAGGCTGACCTCATATCAAGATTTTCCTATTCGGGTCGCGGAATTGGGGACGGTTTATAGATATGAGTTGTCTGGTAGCTTGCACGGGCTTTTCCGTGTCAGAGGTTTTACCCAGGATGATGCTCACATATTTTGTCTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATCTAACAGAAGAATTATTACTTCAATTTGGCTTTAACAAATTTGAAGTCAATCTCTCTACTAGACCACCAAAATCAGTGGGGAATGATGAGATCTGGGAGAAAGCAACATCTGCTCTTAGAGATGCTCTAAGTGATAAGGGGTGGAGCTATCAAATTGATGAAGGTGGTGGAGCCTTTTACGGACCGAAAATCGATATTAAGATCGAGGATGCATTGGGAAGGAAGTGGCAATGCTCAACTATTCAGGCAATGCCTCAACTTATTTTGGTTTTTCAGGTTGATTTTAATCTACCTCAGCGTTTCGACATCACATACGTTAATTCAAACTCGGAAAAGAAGAGACCTATCATGATTCACCGAGCGGTGCTAGGATCCTTGGAGCGATTCTTCGGTATTCTAATTGAAAACTATGCAGGGGATTTTCCACTATGGCTTTCTCCCGTCCAAGTCCGAGTTTTGCCCGTGACGGACTCCCAGCTCCCCTACTGCAAAGAGGTGACCGAAAAACTCAAAGCGGTTGGTGTTCGAGCAGAATTTTGTCACGGGGAGCGGCTGCCTAAGCTTATTAGAAATGCAGAGACCCAAAAGATACCGTTAATGGCGGTTGTTGGCGCCAAGGAAGTCGAGACACAAACTGTGACAGTGAGATCCAGGTTTGGTGGGGAGATAGGGACCATAACAGTTGATGATTTTGTTGGAAAAATCAAGTTTGCCTCGGAGAATAGAACATCATTTTGA

Protein sequence

MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSSVAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIKFASENRTSF
Homology
BLAST of MC04g0273 vs. ExPASy Swiss-Prot
Match: F4IFC5 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2761 PE=2 SV=1)

HSP 1 Score: 1068.5 bits (2762), Expect = 3.0e-311
Identity = 522/675 (77.33%), Postives = 587/675 (86.96%), Query Frame = 0

Query: 7   MASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSSVAVATD 66
           MASS  LL +   +   SS  S  R F+ + T+         F  ++ +GFS+  AVAT+
Sbjct: 1   MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPTR--------QFWPRQRHGFSTVFAVATE 60

Query: 67  SSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126
            + +S           +K E   VVLP+NESS+KLL+IRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61  PAISSSG--------PKKAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKVT 120

Query: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEP 186
           IGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPLLREEVSR+EA++RIMAINEP
Sbjct: 121 IGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINEP 180

Query: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246
           YKMEIL+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK  
Sbjct: 181 YKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKRQ 240

Query: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAI 306
           MLQRIYGTAWE+EEQLKAYLHFKEEAKRRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGAI
Sbjct: 241 MLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAI 300

Query: 307 VRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRP 366
           VR+IIE+ WKK+H + GYDL+YTPHVAKA+LWK SGH DFY ENMY+QM++EDELYQLRP
Sbjct: 301 VRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRP 360

Query: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426
           MNCPYHIL Y+R+  SY+D PIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCL+D
Sbjct: 361 MNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLED 420

Query: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWS 486
           QIKDEIRGVLDLTEE+L +FGFNK+EVNLSTRP KSVG D+IWEKAT ALRDAL DKGWS
Sbjct: 421 QIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGWS 480

Query: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSN 546
           Y++DEGGGAFYGPKID+KIEDALGRKWQCSTI          QVDFNLPQRFDITYV++N
Sbjct: 481 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTI----------QVDFNLPQRFDITYVDTN 540

Query: 547 SEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEK 606
           S+KKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPVQVRVLPVTD+QL +CKEV++K
Sbjct: 541 SDKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKK 600

Query: 607 LKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVD 666
           L+A GVRAE CHGERLPKLIRNAETQKIPLMAVVG KEVET TVTVRSRFGGE+GTI VD
Sbjct: 601 LRACGVRAELCHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVD 649

Query: 667 DFVGKIKFASENRTS 682
           D + KI  A E RT+
Sbjct: 661 DLINKINIAVETRTA 649

BLAST of MC04g0273 vs. ExPASy Swiss-Prot
Match: Q0I902 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) OX=64471 GN=thrS PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 9.1e-223
Identity = 366/592 (61.82%), Postives = 455/592 (76.86%), Query Frame = 0

Query: 90  VVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD-MEPLGDKD 149
           VVLP    SE LL+IRH+ +HVMAMAVQKLFP A+VTIGPW E GFYYDFD  EP  + D
Sbjct: 23  VVLPKTSESENLLKIRHSMSHVMAMAVQKLFPKAQVTIGPWTEAGFYYDFDNPEPFTEAD 82

Query: 150 LKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKEDPITIYHIGNE 209
           LK IKKEM +II R LPL R EVSR+EA+RRI A NEPYK+EILE + E PIT+Y +G +
Sbjct: 83  LKAIKKEMGKIIGRKLPLERIEVSREEAERRIKAQNEPYKLEILERLVE-PITLYTLGEQ 142

Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
           WWDLCAGPHV +T ++N +A ELE+VAGAYWRGDE    LQRIYGTAWET +QL  +   
Sbjct: 143 WWDLCAGPHVANTSELNPKAFELESVAGAYWRGDETKAQLQRIYGTAWETADQLSEHKRR 202

Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHRQRGYDLLY 329
           KEEA RRDHRRLGKDLDLFSI+D+AG GLVFWHP+GA +R +IED W++ H + GY+LLY
Sbjct: 203 KEEALRRDHRRLGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262

Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
           TPHVA   LWKTSGH DFY E+M+  M+V++  YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDERQYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 390 RVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGF 449
           R AELGTVYRYE  G +HGL RVRGFTQDDAH+FCL +QI DEI  +L+LTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILRILNLTERILSTFDF 382

Query: 450 NKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPKIDIKIEDA 509
           + +E+NLST+P K++G+D +WE AT  L +AL  KGW+Y+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 SNYEINLSTKPDKAIGDDAVWELATKGLIEALKRKGWAYKIDEGGGAFYGPKIDLKIEDA 442

Query: 510 LGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERFFG 569
           +GR WQCSTI          Q+DFNLP+RF++ Y+ ++  K+RPIMIHRA+ GSLERFFG
Sbjct: 443 IGRMWQCSTI----------QLDFNLPERFELDYIAADGSKQRPIMIHRAIFGSLERFFG 502

Query: 570 ILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCH-GERLPKLIR 629
           I+ ENYAGDFP WL+P Q+R+LPVTD  L Y +E   +LKA G+RA     G+RL KLIR
Sbjct: 503 IMTENYAGDFPFWLAPEQIRLLPVTDEVLGYAEEFQNQLKAAGIRASIDRSGDRLGKLIR 562

Query: 630 NAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIKFASENR 680
             E  KIP++AV+GAKE E    ++RSR  G++G IT +  +   + A+++R
Sbjct: 563 IGEKMKIPVLAVIGAKEAEQGAASLRSRRDGDLGVITKERLIATAQSANQDR 603

BLAST of MC04g0273 vs. ExPASy Swiss-Prot
Match: Q3AYC6 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) OX=316279 GN=thrS PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 1.2e-222
Identity = 366/592 (61.82%), Postives = 455/592 (76.86%), Query Frame = 0

Query: 90  VVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD-MEPLGDKD 149
           VVLP    SE+LL+IRH+ +HVMAMAVQKLFP+A+VTIGPW E GFYYDFD  EP  + D
Sbjct: 23  VVLPKTSESEQLLKIRHSMSHVMAMAVQKLFPNAQVTIGPWTETGFYYDFDNPEPFTEDD 82

Query: 150 LKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKEDPITIYHIGNE 209
           LK IKKEM +II R LPL R EVSRDEA+ RI A NEPYK+EILE ++E PIT+Y +G++
Sbjct: 83  LKAIKKEMGKIIGRKLPLERIEVSRDEAETRIKAQNEPYKLEILERLQE-PITLYTLGDQ 142

Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
           WWDLCAGPHVE+T +++ +A ELE+VAGAYWRGDE    LQRIYGTAWET EQL  +   
Sbjct: 143 WWDLCAGPHVENTKELHPKAFELESVAGAYWRGDETKAQLQRIYGTAWETPEQLAEHKRR 202

Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHRQRGYDLLY 329
           K EA RRDHRRLGKDL+LFSI+D+AG GLVFWHP+GA +R +IED W++ H + GY+LLY
Sbjct: 203 KVEALRRDHRRLGKDLELFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262

Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
           TPHVA   LWKTSGH DFY E+M+  M V++  YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMQVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 390 RVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGF 449
           R AELGTVYRYE  G +HGL RVRGFTQDDAH+FCL DQI DEI  +LDLTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPDQISDEILRILDLTERILSTFDF 382

Query: 450 NKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPKIDIKIEDA 509
           N +E+NLSTRP KS+G++ +WE AT  L +AL  KGW+++IDEGGGAFYGPKID+KIEDA
Sbjct: 383 NHYEINLSTRPEKSIGSEAVWELATKGLTEALERKGWNFKIDEGGGAFYGPKIDLKIEDA 442

Query: 510 LGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERFFG 569
           +GR WQCSTI          Q+DFNLP+RF + YV ++  K++PIMIHRA+ GSLERFFG
Sbjct: 443 IGRMWQCSTI----------QLDFNLPERFGLDYVAADGSKQQPIMIHRAIFGSLERFFG 502

Query: 570 ILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCH-GERLPKLIR 629
           I+ ENYAGDFP WL+P Q+R+LPVTD   PY ++V  +LK  G+RA     G+RL K+IR
Sbjct: 503 IMTENYAGDFPFWLAPEQIRLLPVTDDVQPYAEQVLNQLKTAGIRATIDQSGDRLGKIIR 562

Query: 630 NAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIKFASENR 680
             E  KIP++AV+GAKE E   +++RSR  G++G  +VD  +   + A+  R
Sbjct: 563 TGEQMKIPVLAVIGAKEAEQNAISLRSRRDGDLGVTSVDALLKAAQRANAER 603

BLAST of MC04g0273 vs. ExPASy Swiss-Prot
Match: A5GJT7 (Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) OX=32051 GN=thrS PE=3 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.6e-222
Identity = 366/602 (60.80%), Postives = 459/602 (76.25%), Query Frame = 0

Query: 82  TQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD- 141
           T       VVLP    +E+LLRIRH+ +HVMAMAVQ+LFP+A+VTIGPW E+GFYYDFD 
Sbjct: 15  TTSASPSPVVLPKTSENEQLLRIRHSMSHVMAMAVQQLFPNARVTIGPWTESGFYYDFDN 74

Query: 142 MEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKEDPI 201
            EP  + DLK IKK M +II++ LPL R EVSR EA+ +I A NEPYK+EILE ++E PI
Sbjct: 75  PEPFTEADLKAIKKGMIKIINKKLPLERVEVSRSEAEEKIKAQNEPYKLEILEGLQE-PI 134

Query: 202 TIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEE 261
           T+Y +G +WWDLCAGPHVE+TG++N +A ELE+VAGAYWRGDE    LQRIYGTAWET E
Sbjct: 135 TLYTLGEDWWDLCAGPHVENTGQLNAKAFELESVAGAYWRGDETKAQLQRIYGTAWETAE 194

Query: 262 QLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHR 321
           QL  +   KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H 
Sbjct: 195 QLAEHKRRKEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEEFWRQAHF 254

Query: 322 QRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRL 381
           + GY+LLYTPHVA   LWKTSGH DFY E+M+  M+V++  YQL+PMNCP+H+L Y  +L
Sbjct: 255 EGGYELLYTPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKL 314

Query: 382 TSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTE 441
            SY++ PIR AELGTVYRYE  G +HGL RVRGFTQDDAH+FCL +QI DEI  +LDLTE
Sbjct: 315 RSYRELPIRWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTE 374

Query: 442 ELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPK 501
            +L  F FN +E+NLSTRP KS+G+D +W+ AT  L +AL  KGW+Y+IDEGGGAFYGPK
Sbjct: 375 RILSTFDFNTYEINLSTRPEKSIGDDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPK 434

Query: 502 IDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVL 561
           ID+KIEDA+GR WQCSTI          Q+DFNLP+RF + Y+ ++  K+RPIMIHRA+ 
Sbjct: 435 IDLKIEDAIGRMWQCSTI----------QLDFNLPERFKLDYIAADGSKQRPIMIHRAIF 494

Query: 562 GSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCH-G 621
           GSLERFFGI+ ENYAGD+PLWL+P QVR+LPVTD   PY + + ++L   GVRA     G
Sbjct: 495 GSLERFFGIMTENYAGDYPLWLAPEQVRLLPVTDEVQPYAESLLDQLTQAGVRATIDRSG 554

Query: 622 ERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIKFASENR 681
           +RL KLIR  E  KIP++AV+GAKE E   V++RSR  G++G + V D +   + A+  R
Sbjct: 555 DRLGKLIRTGEQMKIPVLAVIGAKEAEQNAVSLRSRRDGDLGVVAVADLLRAAQNANSQR 605

BLAST of MC04g0273 vs. ExPASy Swiss-Prot
Match: Q3AKU0 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) OX=110662 GN=thrS PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 5.5e-220
Identity = 359/602 (59.63%), Postives = 456/602 (75.75%), Query Frame = 0

Query: 82  TQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD- 141
           T    S  VVLP    S++LL+IRH+ +HVMAMAVQ+LFP A+VTIGPW E GFYYDFD 
Sbjct: 15  TTPAPSAPVVLPKTSESDQLLKIRHSMSHVMAMAVQQLFPKARVTIGPWTETGFYYDFDN 74

Query: 142 MEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKEDPI 201
            +P  + DLK IKK M +II++ LPL R EVSR+EA+ +I A NEPYK+EIL+ + E PI
Sbjct: 75  PDPFTEADLKAIKKGMIKIINKKLPLQRVEVSRNEAEEKIKAQNEPYKLEILQGLHE-PI 134

Query: 202 TIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEE 261
           T+Y +G +WWDLCAGPHV+ TG++N +A ELE++AGAYWRGDE    LQRIYGTAWE+ E
Sbjct: 135 TLYTLGEDWWDLCAGPHVDHTGQLNAKAFELESLAGAYWRGDETKAQLQRIYGTAWESPE 194

Query: 262 QLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHR 321
           QL  +   KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H 
Sbjct: 195 QLAEHKRRKEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARIRLLIEEFWRQAHF 254

Query: 322 QRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRL 381
           + GY+LLYTPHVA   LWKTSGH DFY E+M+  M+V++  YQL+PMNCP+H+L Y  +L
Sbjct: 255 EGGYELLYTPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKL 314

Query: 382 TSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTE 441
            SY++ PIR AELGTVYRYE  G +HGL RVRGFTQDDAH+FCL +QI DEI  +LDLTE
Sbjct: 315 RSYRELPIRWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTE 374

Query: 442 ELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPK 501
            +L  F F+ +E+NLSTRP KS+G D +W+ AT  L +AL  KGW+Y+IDEGGGAFYGPK
Sbjct: 375 RILSAFDFSNYEINLSTRPEKSIGEDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPK 434

Query: 502 IDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVL 561
           ID+KIEDA+GR WQCSTI          Q+DFNLP+RF++ Y+ ++  K+RPIMIHRA+ 
Sbjct: 435 IDLKIEDAIGRMWQCSTI----------QLDFNLPERFELDYIAADGSKQRPIMIHRAIF 494

Query: 562 GSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCH-G 621
           GSLERFFGI+ ENYAGD+P WL+P QVR+LPVTD   PY +++ ++L   GVRA     G
Sbjct: 495 GSLERFFGIMTENYAGDYPFWLAPEQVRLLPVTDEVQPYAEQLLDQLTKAGVRATVDRSG 554

Query: 622 ERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIKFASENR 681
           +RL KLIR  E  KIP++AV+GAKE E   V++RSR  G++G   V D +   + A+  R
Sbjct: 555 DRLGKLIRTGEQMKIPVLAVIGAKEAEQNAVSLRSRRDGDLGVTAVSDLLSAAQMANSER 605

BLAST of MC04g0273 vs. NCBI nr
Match: XP_022135652.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Momordica charantia])

HSP 1 Score: 1350 bits (3495), Expect = 0.0
Identity = 672/682 (98.53%), Postives = 672/682 (98.53%), Query Frame = 0

Query: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
           MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS
Sbjct: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60

Query: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180

Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
           MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
           HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE
Sbjct: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480

Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
           SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ          VDFNLPQRFDI
Sbjct: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540

Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
           TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600

Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
           KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660

Query: 661 GTITVDDFVGKIKFASENRTSF 682
           GTITVDDFVGKIKFASENRTSF
Sbjct: 661 GTITVDDFVGKIKFASENRTSF 672

BLAST of MC04g0273 vs. NCBI nr
Match: XP_023529641.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1295 bits (3351), Expect = 0.0
Identity = 637/682 (93.40%), Postives = 661/682 (96.92%), Query Frame = 0

Query: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
           MF+LQRMA++TGLLL+PPLVRPISSNVS  RFFLAV+TQFAGNR+KSLF  KKA+GFS+S
Sbjct: 1   MFVLQRMATTTGLLLHPPLVRPISSNVSTSRFFLAVSTQFAGNRNKSLFTRKKASGFSTS 60

Query: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           +AVATDSS  S+STQNE+LV+TQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQ+RI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQKRI 180

Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
           HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKAELWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDAL 480

Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
           SDKGW Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ          VDFNLPQRFDI
Sbjct: 481 SDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540

Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
           TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600

Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
           KEVT+KLKAVGVRAE CHGERLPKLIRNAET+KIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTKKLKAVGVRAEVCHGERLPKLIRNAETKKIPLMAVVGAKEVETQTVTVRSRFGGEI 660

Query: 661 GTITVDDFVGKIKFASENRTSF 682
           GTITVDDFVG+IKFASENRT+F
Sbjct: 661 GTITVDDFVGRIKFASENRTTF 672

BLAST of MC04g0273 vs. NCBI nr
Match: XP_022928511.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita moschata])

HSP 1 Score: 1293 bits (3346), Expect = 0.0
Identity = 635/682 (93.11%), Postives = 660/682 (96.77%), Query Frame = 0

Query: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
           MF+LQRMA++TGLLL+PPLVRPIS NVS  RFFLAV+TQFAGNR+KSL+  KKA+GFS+S
Sbjct: 1   MFVLQRMATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNKSLYPRKKASGFSTS 60

Query: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           +AVATDSS  S+STQN++LV+TQKEESEKVVLPTNESSEKLLR+RHTCAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSISTQNDNLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
           HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKAELWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDAL 480

Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
           SDKGW Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ          VDFNLPQRFDI
Sbjct: 481 SDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540

Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
           TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600

Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
           KEVT+KLKAVGVRAE CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTKKLKAVGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660

Query: 661 GTITVDDFVGKIKFASENRTSF 682
           GTITVDDFVG+IKFASENRT+F
Sbjct: 661 GTITVDDFVGRIKFASENRTTF 672

BLAST of MC04g0273 vs. NCBI nr
Match: XP_022952560.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita moschata])

HSP 1 Score: 1292 bits (3343), Expect = 0.0
Identity = 635/682 (93.11%), Postives = 655/682 (96.04%), Query Frame = 0

Query: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
           MF+LQRMAS+TGLLL+PPLVRPIS NVS  RFFLAVATQFAGNRDKSLFH KKANGFS+S
Sbjct: 1   MFVLQRMASTTGLLLHPPLVRPISPNVSTPRFFLAVATQFAGNRDKSLFHRKKANGFSTS 60

Query: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           +AVATDSS  S+ TQNEDLV+T KEESEK+VLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITI+HIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIFHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
           HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKAT AL+DAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480

Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
           SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ          VDFNLPQRFDI
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540

Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
           TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600

Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
           KEVTEKL+A+GVRAE C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEI
Sbjct: 601 KEVTEKLRAIGVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEI 660

Query: 661 GTITVDDFVGKIKFASENRTSF 682
           GTITVDDF G+IKFASENRT F
Sbjct: 661 GTITVDDFAGRIKFASENRTPF 672

BLAST of MC04g0273 vs. NCBI nr
Match: KAG7011877.1 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1291 bits (3341), Expect = 0.0
Identity = 635/682 (93.11%), Postives = 654/682 (95.89%), Query Frame = 0

Query: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
           MF+LQRMAS+TGLLL+PPLVRPIS NVS  RF LAVATQFAGNRDKSLFH KKANGFS+S
Sbjct: 1   MFVLQRMASTTGLLLHPPLVRPISPNVSTPRFLLAVATQFAGNRDKSLFHRKKANGFSTS 60

Query: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           +AVATDSS  S+ TQNEDLV+T KEESEK+VLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
           HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKAT AL+DAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480

Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
           SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ          VDFNLPQRFDI
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540

Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
           TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600

Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
           KEVTEKL+A+GVRAE C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEI
Sbjct: 601 KEVTEKLRAIGVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEI 660

Query: 661 GTITVDDFVGKIKFASENRTSF 682
           GTITVDDF G+IKFASENRT F
Sbjct: 661 GTITVDDFAGRIKFASENRTPF 672

BLAST of MC04g0273 vs. ExPASy TrEMBL
Match: A0A6J1C3B9 (Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111007557 PE=3 SV=1)

HSP 1 Score: 1350 bits (3495), Expect = 0.0
Identity = 672/682 (98.53%), Postives = 672/682 (98.53%), Query Frame = 0

Query: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
           MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS
Sbjct: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60

Query: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180

Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
           MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
           HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE
Sbjct: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480

Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
           SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ          VDFNLPQRFDI
Sbjct: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540

Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
           TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600

Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
           KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660

Query: 661 GTITVDDFVGKIKFASENRTSF 682
           GTITVDDFVGKIKFASENRTSF
Sbjct: 661 GTITVDDFVGKIKFASENRTSF 672

BLAST of MC04g0273 vs. ExPASy TrEMBL
Match: A0A6J1EKH5 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435298 PE=3 SV=1)

HSP 1 Score: 1293 bits (3346), Expect = 0.0
Identity = 635/682 (93.11%), Postives = 660/682 (96.77%), Query Frame = 0

Query: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
           MF+LQRMA++TGLLL+PPLVRPIS NVS  RFFLAV+TQFAGNR+KSL+  KKA+GFS+S
Sbjct: 1   MFVLQRMATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNKSLYPRKKASGFSTS 60

Query: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           +AVATDSS  S+STQN++LV+TQKEESEKVVLPTNESSEKLLR+RHTCAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSISTQNDNLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
           HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKAELWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDAL 480

Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
           SDKGW Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ          VDFNLPQRFDI
Sbjct: 481 SDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540

Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
           TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600

Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
           KEVT+KLKAVGVRAE CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTKKLKAVGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660

Query: 661 GTITVDDFVGKIKFASENRTSF 682
           GTITVDDFVG+IKFASENRT+F
Sbjct: 661 GTITVDDFVGRIKFASENRTTF 672

BLAST of MC04g0273 vs. ExPASy TrEMBL
Match: A0A6J1GM45 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1)

HSP 1 Score: 1292 bits (3343), Expect = 0.0
Identity = 635/682 (93.11%), Postives = 655/682 (96.04%), Query Frame = 0

Query: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
           MF+LQRMAS+TGLLL+PPLVRPIS NVS  RFFLAVATQFAGNRDKSLFH KKANGFS+S
Sbjct: 1   MFVLQRMASTTGLLLHPPLVRPISPNVSTPRFFLAVATQFAGNRDKSLFHRKKANGFSTS 60

Query: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           +AVATDSS  S+ TQNEDLV+T KEESEK+VLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITI+HIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIFHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
           HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKAT AL+DAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480

Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
           SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ          VDFNLPQRFDI
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540

Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
           TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600

Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
           KEVTEKL+A+GVRAE C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEI
Sbjct: 601 KEVTEKLRAIGVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEI 660

Query: 661 GTITVDDFVGKIKFASENRTSF 682
           GTITVDDF G+IKFASENRT F
Sbjct: 661 GTITVDDFAGRIKFASENRTPF 672

BLAST of MC04g0273 vs. ExPASy TrEMBL
Match: A0A6J1HZS8 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468143 PE=3 SV=1)

HSP 1 Score: 1285 bits (3326), Expect = 0.0
Identity = 633/682 (92.82%), Postives = 652/682 (95.60%), Query Frame = 0

Query: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
           MF+LQRMAS+TGLLL+PPLVRPIS NVS  RFFLAVATQFAGNRDKSLFH KKANGFS+S
Sbjct: 1   MFVLQRMASTTGLLLHPPLVRPISPNVSAPRFFLAVATQFAGNRDKSLFHRKKANGFSTS 60

Query: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           +AVATDSS  S+ TQNEDLV+T KEESEK+VLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSR EAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRGEAQRRI 180

Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKN MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNQMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
           HPKGA+VRSIIEDLWK LHR+RGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKTLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKAT AL+DAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480

Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
           SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ          VDFNLPQRFDI
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540

Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
           TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600

Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
           KEVTEKL+A+GVRAE C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEI
Sbjct: 601 KEVTEKLRAIGVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEI 660

Query: 661 GTITVDDFVGKIKFASENRTSF 682
           GTITVDDF G+IKFASENRT F
Sbjct: 661 GTITVDDFAGRIKFASENRTPF 672

BLAST of MC04g0273 vs. ExPASy TrEMBL
Match: A0A6J1JP39 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111486433 PE=3 SV=1)

HSP 1 Score: 1281 bits (3316), Expect = 0.0
Identity = 633/682 (92.82%), Postives = 656/682 (96.19%), Query Frame = 0

Query: 1   MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
           MF+LQRMA++TGLLL+ PLVRPIS NVS  RFFLAV+TQFAG R+KSLF  KKA+G S+S
Sbjct: 1   MFVLQRMATTTGLLLHLPLVRPISPNVSTSRFFLAVSTQFAGIRNKSLFPRKKASGISTS 60

Query: 61  VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           +AVATDSS  S+STQNE+LV+TQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
           HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKAELWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDAL 480

Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
           SDKGW Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ          VDFNLPQRFDI
Sbjct: 481 SDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540

Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
           TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPY 
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYW 600

Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
           KEVT+KLKAVG+RAE CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTKKLKAVGIRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660

Query: 661 GTITVDDFVGKIKFASENRTSF 682
           GTITVDDFVG+IKFASENRT+F
Sbjct: 661 GTITVDDFVGRIKFASENRTTF 672

BLAST of MC04g0273 vs. TAIR 10
Match: AT2G04842.1 (threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative )

HSP 1 Score: 1068.5 bits (2762), Expect = 2.1e-312
Identity = 522/675 (77.33%), Postives = 587/675 (86.96%), Query Frame = 0

Query: 7   MASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSSVAVATD 66
           MASS  LL +   +   SS  S  R F+ + T+         F  ++ +GFS+  AVAT+
Sbjct: 1   MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPTR--------QFWPRQRHGFSTVFAVATE 60

Query: 67  SSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126
            + +S           +K E   VVLP+NESS+KLL+IRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61  PAISSSG--------PKKAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKVT 120

Query: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEP 186
           IGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPLLREEVSR+EA++RIMAINEP
Sbjct: 121 IGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINEP 180

Query: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246
           YKMEIL+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK  
Sbjct: 181 YKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKRQ 240

Query: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAI 306
           MLQRIYGTAWE+EEQLKAYLHFKEEAKRRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGAI
Sbjct: 241 MLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAI 300

Query: 307 VRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRP 366
           VR+IIE+ WKK+H + GYDL+YTPHVAKA+LWK SGH DFY ENMY+QM++EDELYQLRP
Sbjct: 301 VRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRP 360

Query: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426
           MNCPYHIL Y+R+  SY+D PIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCL+D
Sbjct: 361 MNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLED 420

Query: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWS 486
           QIKDEIRGVLDLTEE+L +FGFNK+EVNLSTRP KSVG D+IWEKAT ALRDAL DKGWS
Sbjct: 421 QIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGWS 480

Query: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSN 546
           Y++DEGGGAFYGPKID+KIEDALGRKWQCSTI          QVDFNLPQRFDITYV++N
Sbjct: 481 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTI----------QVDFNLPQRFDITYVDTN 540

Query: 547 SEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEK 606
           S+KKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPVQVRVLPVTD+QL +CKEV++K
Sbjct: 541 SDKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKK 600

Query: 607 LKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVD 666
           L+A GVRAE CHGERLPKLIRNAETQKIPLMAVVG KEVET TVTVRSRFGGE+GTI VD
Sbjct: 601 LRACGVRAELCHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVD 649

Query: 667 DFVGKIKFASENRTS 682
           D + KI  A E RT+
Sbjct: 661 DLINKINIAVETRTA 649

BLAST of MC04g0273 vs. TAIR 10
Match: AT5G26830.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 401.7 bits (1031), Expect = 1.1e-111
Identity = 221/575 (38.43%), Postives = 338/575 (58.78%), Query Frame = 0

Query: 106 HTCAHVMAMAVQKLFPDAKVTIGPWIE--NGFYYDFDMEPLG--DKDLKKIKKEMERIIS 165
           H+ AH++  A+++ +   ++ IGP      GFYYD     LG  D     I+    +   
Sbjct: 147 HSSAHILGQALEQEY-GCQLCIGPCTTRGEGFYYDGFYGELGLSDNHFPSIEAGAAKAAK 206

Query: 166 RNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKED-PITIYHIGNEWWDLCAGPHVES 225
              P  R EV++D+A       N  +K+E++  +  D  IT+Y  G    DLC GPH+ +
Sbjct: 207 EAQPFERIEVTKDQALEMFSENN--FKVELINGLPADMTITVYRCG-PLVDLCRGPHIPN 266

Query: 226 TGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRL 285
           T  +  +A +    + AYW+GD+    LQR+YG ++  ++QLK YL F EEAK+ DHR L
Sbjct: 267 TSFV--KAFKCLRASSAYWKGDKDRESLQRVYGISYPDQKQLKKYLQFLEEAKKYDHRLL 326

Query: 286 GKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKT 345
           G+  +LF        G  F+ P G  V + + D  K  +  RGY  + TP++   ELW+T
Sbjct: 327 GQKQELF-FSHQLSPGSYFFLPLGTRVYNRLMDFIKNQYWHRGYTEVITPNMYNMELWQT 386

Query: 346 SGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYE 405
           SGH+D Y +NM+   ++E + + L+PMNCP H L ++ R+ SY++ P+R+A+ G ++R E
Sbjct: 387 SGHADNYKDNMFT-FNIEKQEFGLKPMNCPGHCLIFQHRVRSYRELPMRLADFGVLHRNE 446

Query: 406 LSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPP 465
            SG+L GL RVR F QDDAHIFC  +Q+K E++GVL+  + +   FGF  +E+ LSTRP 
Sbjct: 447 ASGALSGLTRVRRFQQDDAHIFCTTEQVKGEVQGVLEFIDYVYKVFGFT-YELKLSTRPE 506

Query: 466 KSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQA 525
           K +G+ E W+KA + L++A+   G    ++EG GAFYGPKIDI + DA+ RK+QC+T+  
Sbjct: 507 KYLGDLETWDKAEADLKEAIEAFGKPLVLNEGDGAFYGPKIDITVSDAMNRKFQCATL-- 566

Query: 526 MPQLILVFQVDFNLPQRFDITYVNSNSEKK-RPIMIHRAVLGSLERFFGILIENYAGDFP 585
                   Q+DF LP RF++ Y   +  KK RP+MIHRAVLGS+ER F IL+E+Y G +P
Sbjct: 567 --------QLDFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWP 626

Query: 586 LWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCHGER-LPKLIRNAETQKIPLMA 645
            W+SP Q  V P+++    Y ++V +++K  G   +    +R + K +R A+  +   + 
Sbjct: 627 FWISPRQAIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYIL 686

Query: 646 VVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIK 674
           VVG  E  T  V+VR R        +++D + + K
Sbjct: 687 VVGETEAATGQVSVRVRDNAAHSVKSIEDLLEEFK 702

BLAST of MC04g0273 vs. TAIR 10
Match: AT1G17960.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 235.3 bits (599), Expect = 1.4e-61
Identity = 128/297 (43.10%), Postives = 180/297 (60.61%), Query Frame = 0

Query: 387 PIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQF 446
           PIR+AE G ++R E SG+L G+ RVR F QDDAHIFC  DQ+++E++GVLD  + +   F
Sbjct: 171 PIRMAEFGVLHRNEDSGALGGMTRVRRFVQDDAHIFCRVDQVEEEVKGVLDFIDYVYRIF 230

Query: 447 GFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPKIDIKIE 506
           GF  +E+ LSTRP   +G+ E W KA + L  AL D G  + I EG GAFYGPKIDI + 
Sbjct: 231 GFT-YELTLSTRPKDHIGDLETWAKAENDLEKALDDFGKPWVIKEGDGAFYGPKIDITVS 290

Query: 507 DALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERF 566
           DA  RK+QC+TI          Q+DF LP  F + Y++  +E + P+MIH AVLGS+ER 
Sbjct: 291 DARNRKFQCATI----------QLDFQLPACFKLKYLSEKNEMEAPVMIHSAVLGSIERM 350

Query: 567 FGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCHGER-LPKL 626
           F IL+E+Y G +P WLSP Q  V  +++    Y K+V +++  VG   +    +R L K 
Sbjct: 351 FAILLEHYKGIWPFWLSPRQAIVCSLSEDCSSYAKQVQKQINEVGYYVDIDESDRSLRKK 410

Query: 627 IRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGE----IGTITVDDFVGKIKFASEN 679
           + +A       + VVG KEV T  VTVR R   E    +  ++++  + + KF + N
Sbjct: 411 VADARDAPYNYILVVGQKEVATGQVTVRLREDPEGRKDLPEMSIESLLDEFKFKTVN 456

BLAST of MC04g0273 vs. TAIR 10
Match: AT1G18130.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 80.1 bits (196), Expect = 7.6e-15
Identity = 46/125 (36.80%), Postives = 67/125 (53.60%), Query Frame = 0

Query: 530 VDFNLPQRFDITYVNSNSEK-KRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVR 589
           VDF+L  RF + Y   N    +R ++IHRAVL S+ER F IL+E Y G FP WLSP Q  
Sbjct: 17  VDFHLRDRFKLEYSAENETMIERSVLIHRAVLSSVERLFAILLEQYKGKFPFWLSPRQAI 76

Query: 590 VLPVTDSQLPYCKEVTEKLKAVGVRAEFCHGER-LPKLIRNAETQKIPLMAVVGAKEVET 649
           V  V++    Y  +V +++   G   +    +R +   ++ A+      + VVG +E  T
Sbjct: 77  VCSVSEDYRSYADKVRDQIHEAGYYVDVDTTDRNVSDKVQEAQVAHYNYILVVGDEEART 136

Query: 650 QTVTV 653
             VTV
Sbjct: 137 GQVTV 141

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IFC53.0e-31177.33Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX... [more]
Q0I9029.1e-22361.82Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) OX=64471 GN=thrS PE=... [more]
Q3AYC61.2e-22261.82Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) OX=316279 GN=thrS PE... [more]
A5GJT71.6e-22260.80Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) OX=32051 GN=thrS PE=... [more]
Q3AKU05.5e-22059.63Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) OX=110662 GN=thrS PE... [more]
Match NameE-valueIdentityDescription
XP_022135652.10.098.53threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Momordica charantia][more]
XP_023529641.10.093.40threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita pepo subsp... [more]
XP_022928511.10.093.11threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita moschata][more]
XP_022952560.10.093.11threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita moschata][more]
KAG7011877.10.093.11Threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
A0A6J1C3B90.098.53Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111007557 PE=3 SV=... [more]
A0A6J1EKH50.093.11Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435298 PE=3 SV=1[more]
A0A6J1GM450.093.11Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1[more]
A0A6J1HZS80.092.82Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468143 PE=3 SV=1[more]
A0A6J1JP390.092.82Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111486433 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G04842.12.1e-31277.33threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative [more]
AT5G26830.11.1e-11138.43Threonyl-tRNA synthetase [more]
AT1G17960.11.4e-6143.10Threonyl-tRNA synthetase [more]
AT1G18130.17.6e-1536.80Class II aaRS and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002320Threonine-tRNA ligase, class IIaPRINTSPR01047TRNASYNTHTHRcoord: 364..392
score: 46.55
coord: 397..420
score: 67.11
coord: 552..565
score: 68.62
coord: 579..591
score: 61.26
coord: 499..527
score: 44.83
IPR002320Threonine-tRNA ligase, class IIaTIGRFAMTIGR00418TIGR00418coord: 105..673
e-value: 3.6E-215
score: 714.1
IPR002320Threonine-tRNA ligase, class IIaPANTHERPTHR11451THREONINE-TRNA LIGASEcoord: 63..680
IPR002320Threonine-tRNA ligase, class IIaHAMAPMF_00184Thr_tRNA_synthcoord: 99..678
score: 32.459484
IPR012947Threonyl/alanyl tRNA synthetase, SADSMARTSM00863tRNA_SAD_4coord: 200..252
e-value: 2.4E-21
score: 86.9
IPR012947Threonyl/alanyl tRNA synthetase, SADPFAMPF07973tRNA_SADcoord: 200..252
e-value: 3.2E-13
score: 49.5
NoneNo IPR availableGENE3D3.30.980.10coord: 103..256
e-value: 9.8E-60
score: 203.4
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 258..577
e-value: 4.0E-118
score: 396.1
NoneNo IPR availableGENE3D3.30.54.20coord: 162..218
e-value: 9.8E-60
score: 203.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..87
NoneNo IPR availablePANTHERPTHR11451:SF44THREONINE--TRNA LIGASE, CHLOROPLASTIC/MITOCHONDRIAL 2coord: 63..680
NoneNo IPR availableCDDcd00860ThrRS_anticodoncoord: 584..673
e-value: 4.00828E-34
score: 123.383
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 574..673
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 276..571
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 356..574
e-value: 1.2E-36
score: 126.4
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 586..673
e-value: 8.5E-19
score: 67.5
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 578..681
e-value: 6.8E-27
score: 95.9
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 300..579
score: 24.811901
IPR033728Threonine-tRNA ligase catalytic core domainCDDcd00771ThrRS_corecoord: 276..584
e-value: 4.93412E-180
score: 511.71
IPR018163Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamilySUPERFAMILY55186ThrRS/AlaRS common domaincoord: 97..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0273.1MC04g0273.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006435 threonyl-tRNA aminoacylation
biological_process GO:0043039 tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004829 threonine-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding