Homology
BLAST of MC04g0273 vs. ExPASy Swiss-Prot
Match:
F4IFC5 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2761 PE=2 SV=1)
HSP 1 Score: 1068.5 bits (2762), Expect = 3.0e-311
Identity = 522/675 (77.33%), Postives = 587/675 (86.96%), Query Frame = 0
Query: 7 MASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSSVAVATD 66
MASS LL + + SS S R F+ + T+ F ++ +GFS+ AVAT+
Sbjct: 1 MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPTR--------QFWPRQRHGFSTVFAVATE 60
Query: 67 SSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126
+ +S +K E VVLP+NESS+KLL+IRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61 PAISSSG--------PKKAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKVT 120
Query: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEP 186
IGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPLLREEVSR+EA++RIMAINEP
Sbjct: 121 IGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINEP 180
Query: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246
YKMEIL+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK
Sbjct: 181 YKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKRQ 240
Query: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAI 306
MLQRIYGTAWE+EEQLKAYLHFKEEAKRRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGAI
Sbjct: 241 MLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAI 300
Query: 307 VRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRP 366
VR+IIE+ WKK+H + GYDL+YTPHVAKA+LWK SGH DFY ENMY+QM++EDELYQLRP
Sbjct: 301 VRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRP 360
Query: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426
MNCPYHIL Y+R+ SY+D PIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCL+D
Sbjct: 361 MNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLED 420
Query: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWS 486
QIKDEIRGVLDLTEE+L +FGFNK+EVNLSTRP KSVG D+IWEKAT ALRDAL DKGWS
Sbjct: 421 QIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGWS 480
Query: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSN 546
Y++DEGGGAFYGPKID+KIEDALGRKWQCSTI QVDFNLPQRFDITYV++N
Sbjct: 481 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTI----------QVDFNLPQRFDITYVDTN 540
Query: 547 SEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEK 606
S+KKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPVQVRVLPVTD+QL +CKEV++K
Sbjct: 541 SDKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKK 600
Query: 607 LKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVD 666
L+A GVRAE CHGERLPKLIRNAETQKIPLMAVVG KEVET TVTVRSRFGGE+GTI VD
Sbjct: 601 LRACGVRAELCHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVD 649
Query: 667 DFVGKIKFASENRTS 682
D + KI A E RT+
Sbjct: 661 DLINKINIAVETRTA 649
BLAST of MC04g0273 vs. ExPASy Swiss-Prot
Match:
Q0I902 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) OX=64471 GN=thrS PE=3 SV=1)
HSP 1 Score: 774.6 bits (1999), Expect = 9.1e-223
Identity = 366/592 (61.82%), Postives = 455/592 (76.86%), Query Frame = 0
Query: 90 VVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD-MEPLGDKD 149
VVLP SE LL+IRH+ +HVMAMAVQKLFP A+VTIGPW E GFYYDFD EP + D
Sbjct: 23 VVLPKTSESENLLKIRHSMSHVMAMAVQKLFPKAQVTIGPWTEAGFYYDFDNPEPFTEAD 82
Query: 150 LKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKEDPITIYHIGNE 209
LK IKKEM +II R LPL R EVSR+EA+RRI A NEPYK+EILE + E PIT+Y +G +
Sbjct: 83 LKAIKKEMGKIIGRKLPLERIEVSREEAERRIKAQNEPYKLEILERLVE-PITLYTLGEQ 142
Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
WWDLCAGPHV +T ++N +A ELE+VAGAYWRGDE LQRIYGTAWET +QL +
Sbjct: 143 WWDLCAGPHVANTSELNPKAFELESVAGAYWRGDETKAQLQRIYGTAWETADQLSEHKRR 202
Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHRQRGYDLLY 329
KEEA RRDHRRLGKDLDLFSI+D+AG GLVFWHP+GA +R +IED W++ H + GY+LLY
Sbjct: 203 KEEALRRDHRRLGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262
Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
TPHVA LWKTSGH DFY E+M+ M+V++ YQL+PMNCP+H+L Y +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDERQYQLKPMNCPFHVLTYASKLRSYRELPI 322
Query: 390 RVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGF 449
R AELGTVYRYE G +HGL RVRGFTQDDAH+FCL +QI DEI +L+LTE +L F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILRILNLTERILSTFDF 382
Query: 450 NKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPKIDIKIEDA 509
+ +E+NLST+P K++G+D +WE AT L +AL KGW+Y+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 SNYEINLSTKPDKAIGDDAVWELATKGLIEALKRKGWAYKIDEGGGAFYGPKIDLKIEDA 442
Query: 510 LGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERFFG 569
+GR WQCSTI Q+DFNLP+RF++ Y+ ++ K+RPIMIHRA+ GSLERFFG
Sbjct: 443 IGRMWQCSTI----------QLDFNLPERFELDYIAADGSKQRPIMIHRAIFGSLERFFG 502
Query: 570 ILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCH-GERLPKLIR 629
I+ ENYAGDFP WL+P Q+R+LPVTD L Y +E +LKA G+RA G+RL KLIR
Sbjct: 503 IMTENYAGDFPFWLAPEQIRLLPVTDEVLGYAEEFQNQLKAAGIRASIDRSGDRLGKLIR 562
Query: 630 NAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIKFASENR 680
E KIP++AV+GAKE E ++RSR G++G IT + + + A+++R
Sbjct: 563 IGEKMKIPVLAVIGAKEAEQGAASLRSRRDGDLGVITKERLIATAQSANQDR 603
BLAST of MC04g0273 vs. ExPASy Swiss-Prot
Match:
Q3AYC6 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) OX=316279 GN=thrS PE=3 SV=1)
HSP 1 Score: 774.2 bits (1998), Expect = 1.2e-222
Identity = 366/592 (61.82%), Postives = 455/592 (76.86%), Query Frame = 0
Query: 90 VVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD-MEPLGDKD 149
VVLP SE+LL+IRH+ +HVMAMAVQKLFP+A+VTIGPW E GFYYDFD EP + D
Sbjct: 23 VVLPKTSESEQLLKIRHSMSHVMAMAVQKLFPNAQVTIGPWTETGFYYDFDNPEPFTEDD 82
Query: 150 LKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKEDPITIYHIGNE 209
LK IKKEM +II R LPL R EVSRDEA+ RI A NEPYK+EILE ++E PIT+Y +G++
Sbjct: 83 LKAIKKEMGKIIGRKLPLERIEVSRDEAETRIKAQNEPYKLEILERLQE-PITLYTLGDQ 142
Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
WWDLCAGPHVE+T +++ +A ELE+VAGAYWRGDE LQRIYGTAWET EQL +
Sbjct: 143 WWDLCAGPHVENTKELHPKAFELESVAGAYWRGDETKAQLQRIYGTAWETPEQLAEHKRR 202
Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHRQRGYDLLY 329
K EA RRDHRRLGKDL+LFSI+D+AG GLVFWHP+GA +R +IED W++ H + GY+LLY
Sbjct: 203 KVEALRRDHRRLGKDLELFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262
Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
TPHVA LWKTSGH DFY E+M+ M V++ YQL+PMNCP+H+L Y +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMQVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322
Query: 390 RVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGF 449
R AELGTVYRYE G +HGL RVRGFTQDDAH+FCL DQI DEI +LDLTE +L F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPDQISDEILRILDLTERILSTFDF 382
Query: 450 NKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPKIDIKIEDA 509
N +E+NLSTRP KS+G++ +WE AT L +AL KGW+++IDEGGGAFYGPKID+KIEDA
Sbjct: 383 NHYEINLSTRPEKSIGSEAVWELATKGLTEALERKGWNFKIDEGGGAFYGPKIDLKIEDA 442
Query: 510 LGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERFFG 569
+GR WQCSTI Q+DFNLP+RF + YV ++ K++PIMIHRA+ GSLERFFG
Sbjct: 443 IGRMWQCSTI----------QLDFNLPERFGLDYVAADGSKQQPIMIHRAIFGSLERFFG 502
Query: 570 ILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCH-GERLPKLIR 629
I+ ENYAGDFP WL+P Q+R+LPVTD PY ++V +LK G+RA G+RL K+IR
Sbjct: 503 IMTENYAGDFPFWLAPEQIRLLPVTDDVQPYAEQVLNQLKTAGIRATIDQSGDRLGKIIR 562
Query: 630 NAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIKFASENR 680
E KIP++AV+GAKE E +++RSR G++G +VD + + A+ R
Sbjct: 563 TGEQMKIPVLAVIGAKEAEQNAISLRSRRDGDLGVTSVDALLKAAQRANAER 603
BLAST of MC04g0273 vs. ExPASy Swiss-Prot
Match:
A5GJT7 (Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) OX=32051 GN=thrS PE=3 SV=1)
HSP 1 Score: 773.9 bits (1997), Expect = 1.6e-222
Identity = 366/602 (60.80%), Postives = 459/602 (76.25%), Query Frame = 0
Query: 82 TQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD- 141
T VVLP +E+LLRIRH+ +HVMAMAVQ+LFP+A+VTIGPW E+GFYYDFD
Sbjct: 15 TTSASPSPVVLPKTSENEQLLRIRHSMSHVMAMAVQQLFPNARVTIGPWTESGFYYDFDN 74
Query: 142 MEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKEDPI 201
EP + DLK IKK M +II++ LPL R EVSR EA+ +I A NEPYK+EILE ++E PI
Sbjct: 75 PEPFTEADLKAIKKGMIKIINKKLPLERVEVSRSEAEEKIKAQNEPYKLEILEGLQE-PI 134
Query: 202 TIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEE 261
T+Y +G +WWDLCAGPHVE+TG++N +A ELE+VAGAYWRGDE LQRIYGTAWET E
Sbjct: 135 TLYTLGEDWWDLCAGPHVENTGQLNAKAFELESVAGAYWRGDETKAQLQRIYGTAWETAE 194
Query: 262 QLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHR 321
QL + KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H
Sbjct: 195 QLAEHKRRKEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEEFWRQAHF 254
Query: 322 QRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRL 381
+ GY+LLYTPHVA LWKTSGH DFY E+M+ M+V++ YQL+PMNCP+H+L Y +L
Sbjct: 255 EGGYELLYTPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKL 314
Query: 382 TSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTE 441
SY++ PIR AELGTVYRYE G +HGL RVRGFTQDDAH+FCL +QI DEI +LDLTE
Sbjct: 315 RSYRELPIRWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTE 374
Query: 442 ELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPK 501
+L F FN +E+NLSTRP KS+G+D +W+ AT L +AL KGW+Y+IDEGGGAFYGPK
Sbjct: 375 RILSTFDFNTYEINLSTRPEKSIGDDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPK 434
Query: 502 IDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVL 561
ID+KIEDA+GR WQCSTI Q+DFNLP+RF + Y+ ++ K+RPIMIHRA+
Sbjct: 435 IDLKIEDAIGRMWQCSTI----------QLDFNLPERFKLDYIAADGSKQRPIMIHRAIF 494
Query: 562 GSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCH-G 621
GSLERFFGI+ ENYAGD+PLWL+P QVR+LPVTD PY + + ++L GVRA G
Sbjct: 495 GSLERFFGIMTENYAGDYPLWLAPEQVRLLPVTDEVQPYAESLLDQLTQAGVRATIDRSG 554
Query: 622 ERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIKFASENR 681
+RL KLIR E KIP++AV+GAKE E V++RSR G++G + V D + + A+ R
Sbjct: 555 DRLGKLIRTGEQMKIPVLAVIGAKEAEQNAVSLRSRRDGDLGVVAVADLLRAAQNANSQR 605
BLAST of MC04g0273 vs. ExPASy Swiss-Prot
Match:
Q3AKU0 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) OX=110662 GN=thrS PE=3 SV=1)
HSP 1 Score: 765.4 bits (1975), Expect = 5.5e-220
Identity = 359/602 (59.63%), Postives = 456/602 (75.75%), Query Frame = 0
Query: 82 TQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD- 141
T S VVLP S++LL+IRH+ +HVMAMAVQ+LFP A+VTIGPW E GFYYDFD
Sbjct: 15 TTPAPSAPVVLPKTSESDQLLKIRHSMSHVMAMAVQQLFPKARVTIGPWTETGFYYDFDN 74
Query: 142 MEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKEDPI 201
+P + DLK IKK M +II++ LPL R EVSR+EA+ +I A NEPYK+EIL+ + E PI
Sbjct: 75 PDPFTEADLKAIKKGMIKIINKKLPLQRVEVSRNEAEEKIKAQNEPYKLEILQGLHE-PI 134
Query: 202 TIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEE 261
T+Y +G +WWDLCAGPHV+ TG++N +A ELE++AGAYWRGDE LQRIYGTAWE+ E
Sbjct: 135 TLYTLGEDWWDLCAGPHVDHTGQLNAKAFELESLAGAYWRGDETKAQLQRIYGTAWESPE 194
Query: 262 QLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHR 321
QL + KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H
Sbjct: 195 QLAEHKRRKEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARIRLLIEEFWRQAHF 254
Query: 322 QRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRL 381
+ GY+LLYTPHVA LWKTSGH DFY E+M+ M+V++ YQL+PMNCP+H+L Y +L
Sbjct: 255 EGGYELLYTPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKL 314
Query: 382 TSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTE 441
SY++ PIR AELGTVYRYE G +HGL RVRGFTQDDAH+FCL +QI DEI +LDLTE
Sbjct: 315 RSYRELPIRWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTE 374
Query: 442 ELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPK 501
+L F F+ +E+NLSTRP KS+G D +W+ AT L +AL KGW+Y+IDEGGGAFYGPK
Sbjct: 375 RILSAFDFSNYEINLSTRPEKSIGEDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPK 434
Query: 502 IDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVL 561
ID+KIEDA+GR WQCSTI Q+DFNLP+RF++ Y+ ++ K+RPIMIHRA+
Sbjct: 435 IDLKIEDAIGRMWQCSTI----------QLDFNLPERFELDYIAADGSKQRPIMIHRAIF 494
Query: 562 GSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCH-G 621
GSLERFFGI+ ENYAGD+P WL+P QVR+LPVTD PY +++ ++L GVRA G
Sbjct: 495 GSLERFFGIMTENYAGDYPFWLAPEQVRLLPVTDEVQPYAEQLLDQLTKAGVRATVDRSG 554
Query: 622 ERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIKFASENR 681
+RL KLIR E KIP++AV+GAKE E V++RSR G++G V D + + A+ R
Sbjct: 555 DRLGKLIRTGEQMKIPVLAVIGAKEAEQNAVSLRSRRDGDLGVTAVSDLLSAAQMANSER 605
BLAST of MC04g0273 vs. NCBI nr
Match:
XP_022135652.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Momordica charantia])
HSP 1 Score: 1350 bits (3495), Expect = 0.0
Identity = 672/682 (98.53%), Postives = 672/682 (98.53%), Query Frame = 0
Query: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS
Sbjct: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
Query: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE
Sbjct: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ VDFNLPQRFDI
Sbjct: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540
Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
Query: 661 GTITVDDFVGKIKFASENRTSF 682
GTITVDDFVGKIKFASENRTSF
Sbjct: 661 GTITVDDFVGKIKFASENRTSF 672
BLAST of MC04g0273 vs. NCBI nr
Match:
XP_023529641.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1295 bits (3351), Expect = 0.0
Identity = 637/682 (93.40%), Postives = 661/682 (96.92%), Query Frame = 0
Query: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
MF+LQRMA++TGLLL+PPLVRPISSNVS RFFLAV+TQFAGNR+KSLF KKA+GFS+S
Sbjct: 1 MFVLQRMATTTGLLLHPPLVRPISSNVSTSRFFLAVSTQFAGNRNKSLFTRKKASGFSTS 60
Query: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
+AVATDSS S+STQNE+LV+TQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61 IAVATDSSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQ+RI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQKRI 180
Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKAELWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDAL 480
Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
SDKGW Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ VDFNLPQRFDI
Sbjct: 481 SDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540
Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600
Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
KEVT+KLKAVGVRAE CHGERLPKLIRNAET+KIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTKKLKAVGVRAEVCHGERLPKLIRNAETKKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
Query: 661 GTITVDDFVGKIKFASENRTSF 682
GTITVDDFVG+IKFASENRT+F
Sbjct: 661 GTITVDDFVGRIKFASENRTTF 672
BLAST of MC04g0273 vs. NCBI nr
Match:
XP_022928511.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita moschata])
HSP 1 Score: 1293 bits (3346), Expect = 0.0
Identity = 635/682 (93.11%), Postives = 660/682 (96.77%), Query Frame = 0
Query: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
MF+LQRMA++TGLLL+PPLVRPIS NVS RFFLAV+TQFAGNR+KSL+ KKA+GFS+S
Sbjct: 1 MFVLQRMATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNKSLYPRKKASGFSTS 60
Query: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
+AVATDSS S+STQN++LV+TQKEESEKVVLPTNESSEKLLR+RHTCAHVMAMAVQKLF
Sbjct: 61 IAVATDSSAPSISTQNDNLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKAELWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDAL 480
Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
SDKGW Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ VDFNLPQRFDI
Sbjct: 481 SDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540
Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600
Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
KEVT+KLKAVGVRAE CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTKKLKAVGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
Query: 661 GTITVDDFVGKIKFASENRTSF 682
GTITVDDFVG+IKFASENRT+F
Sbjct: 661 GTITVDDFVGRIKFASENRTTF 672
BLAST of MC04g0273 vs. NCBI nr
Match:
XP_022952560.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita moschata])
HSP 1 Score: 1292 bits (3343), Expect = 0.0
Identity = 635/682 (93.11%), Postives = 655/682 (96.04%), Query Frame = 0
Query: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
MF+LQRMAS+TGLLL+PPLVRPIS NVS RFFLAVATQFAGNRDKSLFH KKANGFS+S
Sbjct: 1 MFVLQRMASTTGLLLHPPLVRPISPNVSTPRFFLAVATQFAGNRDKSLFHRKKANGFSTS 60
Query: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
+AVATDSS S+ TQNEDLV+T KEESEK+VLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61 IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
AINEPYKMEILESIKEDPITI+HIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIFHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKAT AL+DAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480
Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ VDFNLPQRFDI
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540
Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600
Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
KEVTEKL+A+GVRAE C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEI
Sbjct: 601 KEVTEKLRAIGVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEI 660
Query: 661 GTITVDDFVGKIKFASENRTSF 682
GTITVDDF G+IKFASENRT F
Sbjct: 661 GTITVDDFAGRIKFASENRTPF 672
BLAST of MC04g0273 vs. NCBI nr
Match:
KAG7011877.1 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1291 bits (3341), Expect = 0.0
Identity = 635/682 (93.11%), Postives = 654/682 (95.89%), Query Frame = 0
Query: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
MF+LQRMAS+TGLLL+PPLVRPIS NVS RF LAVATQFAGNRDKSLFH KKANGFS+S
Sbjct: 1 MFVLQRMASTTGLLLHPPLVRPISPNVSTPRFLLAVATQFAGNRDKSLFHRKKANGFSTS 60
Query: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
+AVATDSS S+ TQNEDLV+T KEESEK+VLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61 IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKAT AL+DAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480
Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ VDFNLPQRFDI
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540
Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600
Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
KEVTEKL+A+GVRAE C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEI
Sbjct: 601 KEVTEKLRAIGVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEI 660
Query: 661 GTITVDDFVGKIKFASENRTSF 682
GTITVDDF G+IKFASENRT F
Sbjct: 661 GTITVDDFAGRIKFASENRTPF 672
BLAST of MC04g0273 vs. ExPASy TrEMBL
Match:
A0A6J1C3B9 (Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111007557 PE=3 SV=1)
HSP 1 Score: 1350 bits (3495), Expect = 0.0
Identity = 672/682 (98.53%), Postives = 672/682 (98.53%), Query Frame = 0
Query: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS
Sbjct: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
Query: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE
Sbjct: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ VDFNLPQRFDI
Sbjct: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540
Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
Query: 661 GTITVDDFVGKIKFASENRTSF 682
GTITVDDFVGKIKFASENRTSF
Sbjct: 661 GTITVDDFVGKIKFASENRTSF 672
BLAST of MC04g0273 vs. ExPASy TrEMBL
Match:
A0A6J1EKH5 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435298 PE=3 SV=1)
HSP 1 Score: 1293 bits (3346), Expect = 0.0
Identity = 635/682 (93.11%), Postives = 660/682 (96.77%), Query Frame = 0
Query: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
MF+LQRMA++TGLLL+PPLVRPIS NVS RFFLAV+TQFAGNR+KSL+ KKA+GFS+S
Sbjct: 1 MFVLQRMATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNKSLYPRKKASGFSTS 60
Query: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
+AVATDSS S+STQN++LV+TQKEESEKVVLPTNESSEKLLR+RHTCAHVMAMAVQKLF
Sbjct: 61 IAVATDSSAPSISTQNDNLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKAELWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDAL 480
Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
SDKGW Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ VDFNLPQRFDI
Sbjct: 481 SDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540
Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600
Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
KEVT+KLKAVGVRAE CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTKKLKAVGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
Query: 661 GTITVDDFVGKIKFASENRTSF 682
GTITVDDFVG+IKFASENRT+F
Sbjct: 661 GTITVDDFVGRIKFASENRTTF 672
BLAST of MC04g0273 vs. ExPASy TrEMBL
Match:
A0A6J1GM45 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1)
HSP 1 Score: 1292 bits (3343), Expect = 0.0
Identity = 635/682 (93.11%), Postives = 655/682 (96.04%), Query Frame = 0
Query: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
MF+LQRMAS+TGLLL+PPLVRPIS NVS RFFLAVATQFAGNRDKSLFH KKANGFS+S
Sbjct: 1 MFVLQRMASTTGLLLHPPLVRPISPNVSTPRFFLAVATQFAGNRDKSLFHRKKANGFSTS 60
Query: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
+AVATDSS S+ TQNEDLV+T KEESEK+VLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61 IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
AINEPYKMEILESIKEDPITI+HIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIFHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKAT AL+DAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480
Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ VDFNLPQRFDI
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540
Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600
Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
KEVTEKL+A+GVRAE C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEI
Sbjct: 601 KEVTEKLRAIGVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEI 660
Query: 661 GTITVDDFVGKIKFASENRTSF 682
GTITVDDF G+IKFASENRT F
Sbjct: 661 GTITVDDFAGRIKFASENRTPF 672
BLAST of MC04g0273 vs. ExPASy TrEMBL
Match:
A0A6J1HZS8 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468143 PE=3 SV=1)
HSP 1 Score: 1285 bits (3326), Expect = 0.0
Identity = 633/682 (92.82%), Postives = 652/682 (95.60%), Query Frame = 0
Query: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
MF+LQRMAS+TGLLL+PPLVRPIS NVS RFFLAVATQFAGNRDKSLFH KKANGFS+S
Sbjct: 1 MFVLQRMASTTGLLLHPPLVRPISPNVSAPRFFLAVATQFAGNRDKSLFHRKKANGFSTS 60
Query: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
+AVATDSS S+ TQNEDLV+T KEESEK+VLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61 IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSR EAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRGEAQRRI 180
Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKN MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNQMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
HPKGA+VRSIIEDLWK LHR+RGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKTLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKAT AL+DAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480
Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ VDFNLPQRFDI
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540
Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYC
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYC 600
Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
KEVTEKL+A+GVRAE C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEI
Sbjct: 601 KEVTEKLRAIGVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEI 660
Query: 661 GTITVDDFVGKIKFASENRTSF 682
GTITVDDF G+IKFASENRT F
Sbjct: 661 GTITVDDFAGRIKFASENRTPF 672
BLAST of MC04g0273 vs. ExPASy TrEMBL
Match:
A0A6J1JP39 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111486433 PE=3 SV=1)
HSP 1 Score: 1281 bits (3316), Expect = 0.0
Identity = 633/682 (92.82%), Postives = 656/682 (96.19%), Query Frame = 0
Query: 1 MFILQRMASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSS 60
MF+LQRMA++TGLLL+ PLVRPIS NVS RFFLAV+TQFAG R+KSLF KKA+G S+S
Sbjct: 1 MFVLQRMATTTGLLLHLPLVRPISPNVSTSRFFLAVSTQFAGIRNKSLFPRKKASGISTS 60
Query: 61 VAVATDSSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
+AVATDSS S+STQNE+LV+TQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61 IAVATDSSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
Query: 181 MAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360
HPKGA+VRSIIEDLWKKLHR+RGYDLLYTPHVAKAELWKTSGHSDFY ENMYNQMDVEDE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDAL 480
IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KATSALRDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDAL 480
Query: 481 SDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDI 540
SDKGW Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ VDFNLPQRFDI
Sbjct: 481 SDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ----------VDFNLPQRFDI 540
Query: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYC 600
TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPY
Sbjct: 541 TYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYW 600
Query: 601 KEVTEKLKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
KEVT+KLKAVG+RAE CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI
Sbjct: 601 KEVTKKLKAVGIRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEI 660
Query: 661 GTITVDDFVGKIKFASENRTSF 682
GTITVDDFVG+IKFASENRT+F
Sbjct: 661 GTITVDDFVGRIKFASENRTTF 672
BLAST of MC04g0273 vs. TAIR 10
Match:
AT2G04842.1 (threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative )
HSP 1 Score: 1068.5 bits (2762), Expect = 2.1e-312
Identity = 522/675 (77.33%), Postives = 587/675 (86.96%), Query Frame = 0
Query: 7 MASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSSVAVATD 66
MASS LL + + SS S R F+ + T+ F ++ +GFS+ AVAT+
Sbjct: 1 MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPTR--------QFWPRQRHGFSTVFAVATE 60
Query: 67 SSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126
+ +S +K E VVLP+NESS+KLL+IRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61 PAISSSG--------PKKAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKVT 120
Query: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEP 186
IGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPLLREEVSR+EA++RIMAINEP
Sbjct: 121 IGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINEP 180
Query: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246
YKMEIL+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK
Sbjct: 181 YKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKRQ 240
Query: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAI 306
MLQRIYGTAWE+EEQLKAYLHFKEEAKRRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGAI
Sbjct: 241 MLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAI 300
Query: 307 VRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRP 366
VR+IIE+ WKK+H + GYDL+YTPHVAKA+LWK SGH DFY ENMY+QM++EDELYQLRP
Sbjct: 301 VRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRP 360
Query: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426
MNCPYHIL Y+R+ SY+D PIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCL+D
Sbjct: 361 MNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLED 420
Query: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWS 486
QIKDEIRGVLDLTEE+L +FGFNK+EVNLSTRP KSVG D+IWEKAT ALRDAL DKGWS
Sbjct: 421 QIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGWS 480
Query: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSN 546
Y++DEGGGAFYGPKID+KIEDALGRKWQCSTI QVDFNLPQRFDITYV++N
Sbjct: 481 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTI----------QVDFNLPQRFDITYVDTN 540
Query: 547 SEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEK 606
S+KKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPVQVRVLPVTD+QL +CKEV++K
Sbjct: 541 SDKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKK 600
Query: 607 LKAVGVRAEFCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVD 666
L+A GVRAE CHGERLPKLIRNAETQKIPLMAVVG KEVET TVTVRSRFGGE+GTI VD
Sbjct: 601 LRACGVRAELCHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVD 649
Query: 667 DFVGKIKFASENRTS 682
D + KI A E RT+
Sbjct: 661 DLINKINIAVETRTA 649
BLAST of MC04g0273 vs. TAIR 10
Match:
AT5G26830.1 (Threonyl-tRNA synthetase )
HSP 1 Score: 401.7 bits (1031), Expect = 1.1e-111
Identity = 221/575 (38.43%), Postives = 338/575 (58.78%), Query Frame = 0
Query: 106 HTCAHVMAMAVQKLFPDAKVTIGPWIE--NGFYYDFDMEPLG--DKDLKKIKKEMERIIS 165
H+ AH++ A+++ + ++ IGP GFYYD LG D I+ +
Sbjct: 147 HSSAHILGQALEQEY-GCQLCIGPCTTRGEGFYYDGFYGELGLSDNHFPSIEAGAAKAAK 206
Query: 166 RNLPLLREEVSRDEAQRRIMAINEPYKMEILESIKED-PITIYHIGNEWWDLCAGPHVES 225
P R EV++D+A N +K+E++ + D IT+Y G DLC GPH+ +
Sbjct: 207 EAQPFERIEVTKDQALEMFSENN--FKVELINGLPADMTITVYRCG-PLVDLCRGPHIPN 266
Query: 226 TGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRL 285
T + +A + + AYW+GD+ LQR+YG ++ ++QLK YL F EEAK+ DHR L
Sbjct: 267 TSFV--KAFKCLRASSAYWKGDKDRESLQRVYGISYPDQKQLKKYLQFLEEAKKYDHRLL 326
Query: 286 GKDLDLFSIQDDAGGGLVFWHPKGAIVRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKT 345
G+ +LF G F+ P G V + + D K + RGY + TP++ ELW+T
Sbjct: 327 GQKQELF-FSHQLSPGSYFFLPLGTRVYNRLMDFIKNQYWHRGYTEVITPNMYNMELWQT 386
Query: 346 SGHSDFYGENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYE 405
SGH+D Y +NM+ ++E + + L+PMNCP H L ++ R+ SY++ P+R+A+ G ++R E
Sbjct: 387 SGHADNYKDNMFT-FNIEKQEFGLKPMNCPGHCLIFQHRVRSYRELPMRLADFGVLHRNE 446
Query: 406 LSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPP 465
SG+L GL RVR F QDDAHIFC +Q+K E++GVL+ + + FGF +E+ LSTRP
Sbjct: 447 ASGALSGLTRVRRFQQDDAHIFCTTEQVKGEVQGVLEFIDYVYKVFGFT-YELKLSTRPE 506
Query: 466 KSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQA 525
K +G+ E W+KA + L++A+ G ++EG GAFYGPKIDI + DA+ RK+QC+T+
Sbjct: 507 KYLGDLETWDKAEADLKEAIEAFGKPLVLNEGDGAFYGPKIDITVSDAMNRKFQCATL-- 566
Query: 526 MPQLILVFQVDFNLPQRFDITYVNSNSEKK-RPIMIHRAVLGSLERFFGILIENYAGDFP 585
Q+DF LP RF++ Y + KK RP+MIHRAVLGS+ER F IL+E+Y G +P
Sbjct: 567 --------QLDFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWP 626
Query: 586 LWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCHGER-LPKLIRNAETQKIPLMA 645
W+SP Q V P+++ Y ++V +++K G + +R + K +R A+ + +
Sbjct: 627 FWISPRQAIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYIL 686
Query: 646 VVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIK 674
VVG E T V+VR R +++D + + K
Sbjct: 687 VVGETEAATGQVSVRVRDNAAHSVKSIEDLLEEFK 702
BLAST of MC04g0273 vs. TAIR 10
Match:
AT1G17960.1 (Threonyl-tRNA synthetase )
HSP 1 Score: 235.3 bits (599), Expect = 1.4e-61
Identity = 128/297 (43.10%), Postives = 180/297 (60.61%), Query Frame = 0
Query: 387 PIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQF 446
PIR+AE G ++R E SG+L G+ RVR F QDDAHIFC DQ+++E++GVLD + + F
Sbjct: 171 PIRMAEFGVLHRNEDSGALGGMTRVRRFVQDDAHIFCRVDQVEEEVKGVLDFIDYVYRIF 230
Query: 447 GFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWSYQIDEGGGAFYGPKIDIKIE 506
GF +E+ LSTRP +G+ E W KA + L AL D G + I EG GAFYGPKIDI +
Sbjct: 231 GFT-YELTLSTRPKDHIGDLETWAKAENDLEKALDDFGKPWVIKEGDGAFYGPKIDITVS 290
Query: 507 DALGRKWQCSTIQAMPQLILVFQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERF 566
DA RK+QC+TI Q+DF LP F + Y++ +E + P+MIH AVLGS+ER
Sbjct: 291 DARNRKFQCATI----------QLDFQLPACFKLKYLSEKNEMEAPVMIHSAVLGSIERM 350
Query: 567 FGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEFCHGER-LPKL 626
F IL+E+Y G +P WLSP Q V +++ Y K+V +++ VG + +R L K
Sbjct: 351 FAILLEHYKGIWPFWLSPRQAIVCSLSEDCSSYAKQVQKQINEVGYYVDIDESDRSLRKK 410
Query: 627 IRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGE----IGTITVDDFVGKIKFASEN 679
+ +A + VVG KEV T VTVR R E + ++++ + + KF + N
Sbjct: 411 VADARDAPYNYILVVGQKEVATGQVTVRLREDPEGRKDLPEMSIESLLDEFKFKTVN 456
BLAST of MC04g0273 vs. TAIR 10
Match:
AT1G18130.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 80.1 bits (196), Expect = 7.6e-15
Identity = 46/125 (36.80%), Postives = 67/125 (53.60%), Query Frame = 0
Query: 530 VDFNLPQRFDITYVNSNSEK-KRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPVQVR 589
VDF+L RF + Y N +R ++IHRAVL S+ER F IL+E Y G FP WLSP Q
Sbjct: 17 VDFHLRDRFKLEYSAENETMIERSVLIHRAVLSSVERLFAILLEQYKGKFPFWLSPRQAI 76
Query: 590 VLPVTDSQLPYCKEVTEKLKAVGVRAEFCHGER-LPKLIRNAETQKIPLMAVVGAKEVET 649
V V++ Y +V +++ G + +R + ++ A+ + VVG +E T
Sbjct: 77 VCSVSEDYRSYADKVRDQIHEAGYYVDVDTTDRNVSDKVQEAQVAHYNYILVVGDEEART 136
Query: 650 QTVTV 653
VTV
Sbjct: 137 GQVTV 141
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
F4IFC5 | 3.0e-311 | 77.33 | Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX... | [more] |
Q0I902 | 9.1e-223 | 61.82 | Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) OX=64471 GN=thrS PE=... | [more] |
Q3AYC6 | 1.2e-222 | 61.82 | Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) OX=316279 GN=thrS PE... | [more] |
A5GJT7 | 1.6e-222 | 60.80 | Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) OX=32051 GN=thrS PE=... | [more] |
Q3AKU0 | 5.5e-220 | 59.63 | Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) OX=110662 GN=thrS PE... | [more] |
Match Name | E-value | Identity | Description | |
XP_022135652.1 | 0.0 | 98.53 | threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Momordica charantia] | [more] |
XP_023529641.1 | 0.0 | 93.40 | threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita pepo subsp... | [more] |
XP_022928511.1 | 0.0 | 93.11 | threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita moschata] | [more] |
XP_022952560.1 | 0.0 | 93.11 | threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita moschata] | [more] |
KAG7011877.1 | 0.0 | 93.11 | Threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita argyrosperma su... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1C3B9 | 0.0 | 98.53 | Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111007557 PE=3 SV=... | [more] |
A0A6J1EKH5 | 0.0 | 93.11 | Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435298 PE=3 SV=1 | [more] |
A0A6J1GM45 | 0.0 | 93.11 | Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1 | [more] |
A0A6J1HZS8 | 0.0 | 92.82 | Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468143 PE=3 SV=1 | [more] |
A0A6J1JP39 | 0.0 | 92.82 | Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111486433 PE=3 SV=1 | [more] |