MC04g0196 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0196 vs. ExPASy Swiss-Prot
Match: Q9SDQ4 (Glutamate receptor 3.7 OS=Arabidopsis thaliana OX=3702 GN=GLR3.7 PE=2 SV=2)

HSP 1 Score: 968.8 bits (2503), Expect = 4.5e-281
Identity = 490/901 (54.38%), Postives = 649/901 (72.03%), Query Frame = 0

Query: 6   ALALRTLVWLLLTGPIWCQKP-AVNIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDG 65
           ++A+  L+ ++L  P+ CQ+P  VNIGAVF F+SVIGRAAK A+EAA+S +N D   L  
Sbjct: 8   SVAITALIVVILVVPMDCQRPQLVNIGAVFAFDSVIGRAAKVALEAAVSDVNNDKSFLKE 67

Query: 66  TQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGA 125
           T+L+LLMED+ C+ F G+ GA ++LEKEVVA+IGP SS VAH IS I  GL  P VS+ A
Sbjct: 68  TELRLLMEDSACNVFRGSFGAFELLEKEVVAMIGPISSSVAHTISDIAKGLHFPLVSFAA 127

Query: 126 TDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDEL 185
           TDP+LS+LQ PFFLRT  +D++QM+A+ DLI +YGWKEVI++Y DD+ GRNG+S+L DEL
Sbjct: 128 TDPTLSALQFPFFLRTTPNDAHQMSALVDLINFYGWKEVISVYSDDELGRNGVSALDDEL 187

Query: 186 QKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLDMMT 245
            KK  RIS++  L   ++   +T ALN SK +GPRVY++H GPDP LRIF IAQKL MMT
Sbjct: 188 YKKRSRISYKVPLSVHSDEKFLTNALNKSKSIGPRVYILHFGPDPLLRIFDIAQKLQMMT 247

Query: 246 SNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSE-----GKRALADSVMNV 305
             YVW ATDWL+ TLDS S  +  +L  L GVVGLR H P+S        +  ++  MN 
Sbjct: 248 HEYVWLATDWLSVTLDSLS--DKGTLKRLEGVVGLRQHIPESVKMEHFTHKLQSNRSMNA 307

Query: 306 YGLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRII 365
           Y L+AYD+VW++A  +++ L E  NITFS   K+  +  + + L K+K F+ G  LL  +
Sbjct: 308 YALHAYDTVWMIAHGIEELLNEGINITFSYSEKLLHARGTKLHLEKIKFFNSGELLLEKL 367

Query: 366 MGTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQE 425
           +  NFTG++G++QF + RN+I   Y++IN++    HTVG+WS   G  V  P+    +++
Sbjct: 368 LKVNFTGIAGQVQFGSGRNVIGCDYEIINVNKTDVHTVGFWSKNGGFSVVAPKTRHSQKK 427

Query: 426 DYSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVT-QLNNSNVVRG 485
                ++K+  + WPGG  E PRGWVIAD+  PL+I  P+R SFV+FVT + N+S+ ++G
Sbjct: 428 TSFVSDEKLGDITWPGGGREKPRGWVIADSADPLKIVVPRRVSFVEFVTEEKNSSHRIQG 487

Query: 486 YTIDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTK 545
           + IDVF EALKF+PY VPY F PFG+G ++PNY+ L+Q V D V+DAAVGDIAIV +R+K
Sbjct: 488 FCIDVFIEALKFVPYSVPYIFEPFGNGHSSPNYNHLIQMVTDGVYDAAVGDIAIVPSRSK 547

Query: 546 IVDFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRIN 605
           +VDFSQPY +TGL++V P  D  ++ W+FLRPFT  +WCV   SF++I +VIWILEHRIN
Sbjct: 548 LVDFSQPYASTGLVVVIPANDD-NATWIFLRPFTSRLWCVVLVSFLVIAVVIWILEHRIN 607

Query: 606 DHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSI 665
           + FRGPP+RQ+ TM LFSFSTLFK NQE TIS L+RLVM+VWLFLL+V+T+SYTA+LTSI
Sbjct: 608 EDFRGPPRRQLSTMLLFSFSTLFKRNQEDTISNLARLVMIVWLFLLMVLTASYTANLTSI 667

Query: 666 LTIQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALR 725
           LT+QQLPS+ITGID L AS +PIGYQ G+F   YLT SL + RSRLV L + E+YE+AL+
Sbjct: 668 LTVQQLPSAITGIDSLRASEVPIGYQAGTFTLEYLTYSLGMARSRLVPLDSTEEYEKALK 727

Query: 726 VGPKG-GGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAIL 785
           +GP   GGVAAI+DELPY+ELFL+    F ++G+PF   GWGFAF+R+S LA+DMSTAIL
Sbjct: 728 LGPTNWGGVAAIVDELPYIELFLAERTGFKIVGEPFMHRGWGFAFKRDSPLAIDMSTAIL 787

Query: 786 RLSESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLL 845
           +LSE+ KLQE+   W CK  C G+     EP+QLHL SF GLYL+C  I+V+A  VF+L 
Sbjct: 788 KLSETRKLQEIRKKWLCKTNCAGKSNWNPEPNQLHLKSFKGLYLVCIAITVSAFLVFVLR 847

Query: 846 LVRQYIRYKRHRRHFEETTPSPITSHTSCTRS-IQSFISFIDEKEEAIKNLFRGSHGGTQ 898
           ++RQ++RY+R  R       S   S T   R  +  F+ F+DEKEEAIK +FR S     
Sbjct: 848 MIRQFVRYRRMERTSSMPRASWSASPTLRLRELVFDFVEFVDEKEEAIKRMFRRSDDSNN 905

BLAST of MC04g0196 vs. ExPASy Swiss-Prot
Match: Q8GXJ4 (Glutamate receptor 3.4 OS=Arabidopsis thaliana OX=3702 GN=GLR3.4 PE=1 SV=2)

HSP 1 Score: 877.9 bits (2267), Expect = 1.0e-253
Identity = 444/883 (50.28%), Postives = 609/883 (68.97%), Query Frame = 0

Query: 20  PIWCQKPAVNIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDGTQLKLLMEDANCSGF 79
           P+  +  +VN+GA+FT++S IGRAAKPA++AA+  +NAD  +L G +L ++ +D+NCSGF
Sbjct: 53  PLRQRPSSVNVGALFTYDSFIGRAAKPAVKAAMDDVNADQSVLKGIKLNIIFQDSNCSGF 112

Query: 80  LGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGATDPSLSSLQLPFFLR 139
           +GT+GALQ++E +VVA IGPQSS +AH+IS + N L VP +S+GATDP+LSSLQ P+FLR
Sbjct: 113 IGTMGALQLMENKVVAAIGPQSSGIAHMISYVANELHVPLLSFGATDPTLSSLQFPYFLR 172

Query: 140 TALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDELQKKMCRISHQFALPS 199
           T  +D +QM A+AD + Y GW++VIAI++DD+ GRNGIS LGD L KK  RIS++ A+  
Sbjct: 173 TTQNDYFQMHAIADFLSYSGWRQVIAIFVDDECGRNGISVLGDVLAKKRSRISYKAAITP 232

Query: 200 LANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLDMMTSNYVWFATDWLAATL 259
            A+ S I + L +  L+  RV+VVHV PD  L +F +A+ L MM S YVW ATDWL   +
Sbjct: 233 GADSSSIRDLLVSVNLMESRVFVVHVNPDSGLNVFSVAKSLGMMASGYVWIATDWLPTAM 292

Query: 260 DSFSPTNSASLDILHGVVGLRPHTPDSEGKRAL--------ADSVMNVYGLYAYDSVWVV 319
           DS    +S ++D+L GVV  R +T +S  KR           +   N Y +YAYDSVW+V
Sbjct: 293 DSMEHVDSDTMDLLQGVVAFRHYTIESSVKRQFMARWKNLRPNDGFNSYAMYAYDSVWLV 352

Query: 320 ARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRIIMGTNFTGLSGRI 379
           ARA+D F +EN NITFS+   +  ++ S IQL  L VF+ G   ++II+G N TG++G I
Sbjct: 353 ARALDVFFRENNNITFSNDPNLHKTNGSTIQLSALSVFNEGEKFMKIILGMNHTGVTGPI 412

Query: 380 QFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQEDYSRLNQKIESV 439
           QFD+DRN +N +Y+V+N++     TVGYWSN+SGL V  PE L  +  + S  NQ+++ +
Sbjct: 413 QFDSDRNRVNPAYEVLNLEGTAPRTVGYWSNHSGLSVVHPETLYSRPPNTSTANQRLKGI 472

Query: 440 VWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYTIDVFKEALKFI 499
           ++PG  T+ PRGWV  + G+PLRI  P R S+ D+V++  N   VRGY IDVF+ A++ +
Sbjct: 473 IYPGEVTKPPRGWVFPNNGKPLRIGVPNRVSYTDYVSKDKNPPGVRGYCIDVFEAAIELL 532

Query: 500 PYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIVDFSQPYTTTGL 559
           PY VP  ++ +GDG+ NP+YD LV  V    FD AVGDI IVTNRT+ VDF+QP+  +GL
Sbjct: 533 PYPVPRTYILYGDGKRNPSYDNLVNEVVADNFDVAVGDITIVTNRTRYVDFTQPFIESGL 592

Query: 560 IIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDHFRGPPKRQIIT 619
           ++VAPV+++KSS W FL+PFT+EMW VT   F+ +G ++WILEHR N  FRGPP+RQ+IT
Sbjct: 593 VVVAPVKEAKSSPWSFLKPFTIEMWAVTGGFFLFVGAMVWILEHRFNQEFRGPPRRQLIT 652

Query: 620 MCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILTIQQLPSSITGI 679
           +  FSFST+F +++E T+S L R V+++WLF++L+I SSYTASLTSILTI+QL S I GI
Sbjct: 653 IFWFSFSTMFFSHRENTVSSLGRFVLIIWLFVVLIINSSYTASLTSILTIRQLTSRIEGI 712

Query: 680 DDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVGPKGGGVAAIID 739
           D L+ SN PIG Q G+FA +YL + L I  SR+V L   E Y  AL+ GP  GGVAAI+D
Sbjct: 713 DSLVTSNEPIGVQDGTFARNYLINELNILPSRIVPLKDEEQYLSALQRGPNAGGVAAIVD 772

Query: 740 ELPYVELFLSNTK-EFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLSESGKLQELHD 799
           ELPY+E+ L+N+  +F  +GQ FTR+GWGFAFQR+S LAVDMSTAIL+LSE G+L+++H 
Sbjct: 773 ELPYIEVLLTNSNCKFRTVGQEFTRTGWGFAFQRDSPLAVDMSTAILQLSEEGELEKIHR 832

Query: 800 SWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVRQYIRY----K 859
            W         +   SE  QL L SFWGL+L+CGI    AL VF   +  QY R      
Sbjct: 833 KWLNYKHECSMQISNSEDSQLSLKSFWGLFLICGITCFMALTVFFWRVFWQYQRLLPESA 892

Query: 860 RHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFR 890
              R  E + PS  +   S   S +  I  +D++E  IK + +
Sbjct: 893 DEERAGEVSEPSR-SGRGSRAPSFKELIKVVDKREAEIKEILK 934

BLAST of MC04g0196 vs. ExPASy Swiss-Prot
Match: Q9SW97 (Glutamate receptor 3.5 OS=Arabidopsis thaliana OX=3702 GN=GLR3.5 PE=2 SV=2)

HSP 1 Score: 869.8 bits (2246), Expect = 2.8e-251
Identity = 435/904 (48.12%), Postives = 614/904 (67.92%), Query Frame = 0

Query: 27  AVNIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDGTQLKLLMEDANCSGFLGTVGAL 86
           +VN+GA+FT++S IGRAAK A  AAI  INAD  IL GT+L ++ +D NCSGF+GT+GAL
Sbjct: 47  SVNVGALFTYDSFIGRAAKLAFVAAIEDINADQSILRGTKLNIVFQDTNCSGFVGTMGAL 106

Query: 87  QVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGATDPSLSSLQLPFFLRTALSDSY 146
           Q++E +VVA IGPQSS + H+IS + N L VP +S+ ATDP+LSSLQ P+FLRT  +D +
Sbjct: 107 QLMENKVVAAIGPQSSGIGHIISHVANELHVPFLSFAATDPTLSSLQYPYFLRTTQNDYF 166

Query: 147 QMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDELQKKMCRISHQFALPSLANLSQI 206
           QM A+ D + Y+ W+EV+AI++DD+YGRNGIS LGD L KK  +IS++ A P  A+ S I
Sbjct: 167 QMNAITDFVSYFRWREVVAIFVDDEYGRNGISVLGDALAKKRAKISYKAAFPPGADNSSI 226

Query: 207 TEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLDMMTSNYVWFATDWLAATLDSFSPTN 266
           ++ L +  L+  R++VVHV PD  L IF +A+ L MM S YVW  TDWL   LDS  P +
Sbjct: 227 SDLLASVNLMESRIFVVHVNPDSGLNIFSVAKSLGMMGSGYVWITTDWLLTALDSMEPLD 286

Query: 267 SASLDILHGVVGLRPHTPDSEGKRAL--------------ADSVMNVYGLYAYDSVWVVA 326
             +LD+L GVV  R +TP+S+ KR                +D   N Y LYAYDSVW+VA
Sbjct: 287 PRALDLLQGVVAFRHYTPESDNKRQFKGRWKNLRFKESLKSDDGFNSYALYAYDSVWLVA 346

Query: 327 RAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRIIMGTNFTGLSGRIQ 386
           RA+D F  +   +TFS+   +  ++ SGI+L KL +F+ G   L++I+  N+TGL+G+I+
Sbjct: 347 RALDVFFSQGNTVTFSNDPSLRNTNDSGIKLSKLHIFNEGERFLQVILEMNYTGLTGQIE 406

Query: 387 FDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQEDYSRLNQKIESVV 446
           F++++N IN +YD++NI S G   VGYWSN++G  V+PPE L  K  + S  +Q++  ++
Sbjct: 407 FNSEKNRINPAYDILNIKSTGPLRVGYWSNHTGFSVAPPETLYSKPSNTSAKDQRLNEII 466

Query: 447 WPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYTIDVFKEALKFIP 506
           WPG   + PRGWV  + G+PL+I  P R S+ ++ ++  N   V+G+ ID+F+ A++ +P
Sbjct: 467 WPGEVIKPPRGWVFPENGKPLKIGVPNRVSYKNYASKDKNPLGVKGFCIDIFEAAIQLLP 526

Query: 507 YDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIVDFSQPYTTTGLI 566
           Y VP  ++ +GDG+ NP+YD L+  VA ++FD AVGD+ I+TNRTK VDF+QP+  +GL+
Sbjct: 527 YPVPRTYILYGDGKKNPSYDNLISEVAANIFDVAVGDVTIITNRTKFVDFTQPFIESGLV 586

Query: 567 IVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDHFRGPPKRQIITM 626
           +VAPV+ +KSS W FL+PFT+EMW VT A F+ +G VIWILEHR N+ FRGPP+RQIIT+
Sbjct: 587 VVAPVKGAKSSPWSFLKPFTIEMWAVTGALFLFVGAVIWILEHRFNEEFRGPPRRQIITV 646

Query: 627 CLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILTIQQLPSSITGID 686
             FSFST+F +++E T+S L R V+LVWLF++L+I SSYTASLTSILT+QQL S I G+D
Sbjct: 647 FWFSFSTMFFSHRENTVSTLGRFVLLVWLFVVLIINSSYTASLTSILTVQQLTSRIEGMD 706

Query: 687 DLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVGPKGGGVAAIIDE 746
            LIASN PIG Q G+FA+ +L + L I  SR++ L   E+Y  AL+ GP+GGGVAAI+DE
Sbjct: 707 TLIASNEPIGVQDGTFAWKFLVNELNIAPSRIIPLKDEEEYLSALQRGPRGGGVAAIVDE 766

Query: 747 LPYVELFLSNTK-EFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLSESGKLQELHDS 806
           LPY++  LSN+  +F  +GQ FTR+GWGFAFQR+S LAVDMSTAIL+L+E GKL+++   
Sbjct: 767 LPYIKALLSNSNCKFRTVGQEFTRTGWGFAFQRDSPLAVDMSTAILQLAEEGKLEKIRKK 826

Query: 807 WFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVRQYIRYK-RHRR 866
           W         +   +E  Q+ + SFWGL+L+CG++   AL +F   +  QY R +     
Sbjct: 827 WLTYDHECTMQISDTENYQISVQSFWGLFLICGVVWFIALTLFCWKVFWQYQRLRPEESD 886

Query: 867 HFEETTPSPITSHTSCTR--SIQSFISFIDEKEEAIKNLFRGSHG-----GTQNGDNLQK 908
             +  +    +S     R  S +  I  +D++E  IK + +         G  + +N Q 
Sbjct: 887 EVQARSEEAGSSRGKSLRAVSFKDLIKVVDKREAEIKEMLKEKSSKKLKDGQSSAENSQS 946

BLAST of MC04g0196 vs. ExPASy Swiss-Prot
Match: Q93YT1 (Glutamate receptor 3.2 OS=Arabidopsis thaliana OX=3702 GN=GLR3.2 PE=1 SV=2)

HSP 1 Score: 866.7 bits (2238), Expect = 2.4e-250
Identity = 448/873 (51.32%), Postives = 606/873 (69.42%), Query Frame = 0

Query: 28  VNIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDGTQLKLLMEDANCSGFLGTVGALQ 87
           V++GA+F+  ++ G     AM+AA   +N+DP  L G++L++   DA  +GFL  +GALQ
Sbjct: 30  VDVGAIFSLGTLQGEVTNIAMKAAEEDVNSDPSFLGGSKLRITTYDAKRNGFLTIMGALQ 89

Query: 88  VLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGATDPSLSSLQLPFFLRTALSDSYQ 147
            +E + VAIIGPQ+S++AHV+S + N L VP +S+ A DPSLS+LQ PFF++TA SD + 
Sbjct: 90  FMETDAVAIIGPQTSIMAHVLSHLANELSVPMLSFTALDPSLSALQFPFFVQTAPSDLFL 149

Query: 148 MAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDELQKKMCRISHQFALPSLANLSQIT 207
           M A+A++I YYGW EVIA+Y DDD  RNGI++LGDEL+ + C+IS++  LP    ++   
Sbjct: 150 MRAIAEMISYYGWSEVIALYNDDDNSRNGITALGDELEGRRCKISYKAVLPLDVVITSPR 209

Query: 208 EALNN-SKLLG--PRVYVVHVGPDPQLRIFKIAQKLDMMTSNYVWFATDWLAATLDSFSP 267
           E +N   K+ G   RV +V+  P    +IF+ AQKL MM   YVW AT WL + LDS +P
Sbjct: 210 EIINELVKIQGMESRVIIVNTFPKTGKKIFEEAQKLGMMEKGYVWIATTWLTSLLDSVNP 269

Query: 268 TNSASLDILHGVVGLRPHTPDSEGKR-------ALADSV--MNVYGLYAYDSVWVVARAV 327
             + + + L GV+ LR HTP+S+ K+        L++    +NVYGLYAYD+VW++ARAV
Sbjct: 270 LPAKTAESLRGVLTLRIHTPNSKKKKDFVARWNKLSNGTVGLNVYGLYAYDTVWIIARAV 329

Query: 328 DKFLKENGNITFSSQGKVFGSSASG-IQLGKLKVFDGGSDLLRIIMGTNFTGLSGRIQFD 387
            + L    NI+FSS  K+      G + LG L +FD GS  L  I+ TN TG++G+IQF 
Sbjct: 330 KRLLDSRANISFSSDPKLTSMKGGGSLNLGALSIFDQGSQFLDYIVNTNMTGVTGQIQFL 389

Query: 388 ADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQEDYSRLNQKIESVVWP 447
            DR++I  SYD+IN+   GF  +GYWSN+SGL + PPE L  K  + S  NQ + +V WP
Sbjct: 390 PDRSMIQPSYDIINVVDDGFRQIGYWSNHSGLSIIPPESLYKKLSNRSSSNQHLNNVTWP 449

Query: 448 GGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYTIDVFKEALKFIPYD 507
           GG +ETPRGWV  + GR LRI  P R SF +FV++L+ SN V+GY IDVF+ A+K I Y 
Sbjct: 450 GGTSETPRGWVFPNNGRRLRIGVPDRASFKEFVSRLDGSNKVQGYAIDVFEAAVKLISYP 509

Query: 508 VPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIVDFSQPYTTTGLIIV 567
           VP+EFV FGDG  NPN++E V +V   VFDA VGDIAIVT RT+IVDF+QPY  +GL++V
Sbjct: 510 VPHEFVLFGDGLKNPNFNEFVNNVTIGVFDAVVGDIAIVTKRTRIVDFTQPYIESGLVVV 569

Query: 568 APVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDHFRGPPKRQIITMCL 627
           APV     + W FLRPFT  MW VTAA F+I+G VIWILEHRIND FRGPP++QI+T+  
Sbjct: 570 APVTKLNDTPWAFLRPFTPPMWAVTAAFFLIVGSVIWILEHRINDEFRGPPRKQIVTILW 629

Query: 628 FSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILTIQQLPSSITGIDDL 687
           FSFST+F +++E T+S L R V+L+WLF++L+ITSSYTASLTSILT+QQL S I G+D L
Sbjct: 630 FSFSTMFFSHRENTVSTLGRAVLLIWLFVVLIITSSYTASLTSILTVQQLNSPIRGVDTL 689

Query: 688 IASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVGPKGGGVAAIIDELP 747
           I+S+  +G+QVGS+A +Y+ D L I RSRLV L +P++Y  AL    + G VAAI+DE P
Sbjct: 690 ISSSGRVGFQVGSYAENYMIDELNIARSRLVPLGSPKEYAAAL----QNGTVAAIVDERP 749

Query: 748 YVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLSESGKLQELHDSWFC 807
           YV+LFLS    F + GQ FTRSGWGFAF R+S LA+DMSTAIL LSE+G+LQ++HD W  
Sbjct: 750 YVDLFLSEFCGFAIRGQEFTRSGWGFAFPRDSPLAIDMSTAILGLSETGQLQKIHDKWLS 809

Query: 808 KLGCPGERRGKSEPD--QLHLISFWGLYLLCGIISVAALFVFLLLLVRQYIRYKRHRRHF 867
           +  C       S+ D  QL L SFWGL+L+CGI    ALF++   +VR + R+ ++    
Sbjct: 810 RSNCSNLNGSVSDEDSEQLKLRSFWGLFLVCGISCFIALFIYFFKIVRDFFRHGKYDE-- 869

Query: 868 EETTPSPITSHTSCTRSIQSFISFIDEKEEAIK 886
           E T PSP    +S ++S+Q+F+++ DEKE+  K
Sbjct: 870 EATVPSP---ESSRSKSLQTFLAYFDEKEDESK 893

BLAST of MC04g0196 vs. ExPASy Swiss-Prot
Match: Q9C8E7 (Glutamate receptor 3.3 OS=Arabidopsis thaliana OX=3702 GN=GLR3.3 PE=1 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 2.9e-248
Identity = 438/910 (48.13%), Postives = 611/910 (67.14%), Query Frame = 0

Query: 24  QKP-AVNIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDGTQLKLLMEDANCSGFLGT 83
           +KP  V IG++F+F+SVIG+ AK A++ A+  +N++P IL GT+  + M+++NCSGF+G 
Sbjct: 24  EKPKVVKIGSIFSFDSVIGKVAKIAIDEAVKDVNSNPDILSGTKFSVSMQNSNCSGFMGM 83

Query: 84  VGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGATDPSLSSLQLPFFLRTAL 143
           V AL+ +EK++V IIGPQ SVVAH+IS + N L+VP +S+  TDP +S LQ P+F+RT  
Sbjct: 84  VEALRFMEKDIVGIIGPQCSVVAHMISHMANELRVPLLSFAVTDPVMSPLQFPYFIRTTQ 143

Query: 144 SDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDELQKKMCRISHQFAL--PSL 203
           SD YQM A+A ++ +YGWKEVIA+++DDD+GRNG+++L D+L  +  RI+++  L   + 
Sbjct: 144 SDLYQMDAIASIVDFYGWKEVIAVFVDDDFGRNGVAALNDKLASRRLRITYKAGLHPDTA 203

Query: 204 ANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLDMMTSNYVWFATDWLAATLD 263
            N ++I   L    LL PR+ V+HV  +    +FK A+ L MM + YVW ATDWL+  LD
Sbjct: 204 VNKNEIMNMLIKIMLLQPRIVVIHVYSELGFAVFKEAKYLGMMGNGYVWIATDWLSTNLD 263

Query: 264 SFSPTNSASLDILHGVVGLRPHTPDSEGKRAL---------ADSVMNVYGLYAYDSVWVV 323
           S SP  +  L+ + GV+ LRPHTPDS+ KR           A   +N YGLYAYDSV ++
Sbjct: 264 SSSPLPAERLETIQGVLVLRPHTPDSDFKREFFKRWRKMSGASLALNTYGLYAYDSVMLL 323

Query: 324 ARAVDKFLKENGNITFSSQGKVFGSSASG-IQLGKLKVFDGGSDLLRIIMGTNFTGLSGR 383
           AR +DKF K+ GNI+FS+   +     SG + L  + VFDGG  LL+ I+GT   GL+G+
Sbjct: 324 ARGLDKFFKDGGNISFSNHSMLNTLGKSGNLNLEAMTVFDGGEALLKDILGTRMVGLTGQ 383

Query: 384 IQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQEDYSRLNQKIES 443
           +QF  DR+    +YD+IN+   G   +GYWSN+SGL    PE L  K++     + K++ 
Sbjct: 384 LQFTPDRSRTRPAYDIINVAGTGVRQIGYWSNHSGLSTVLPELLYTKEKPNMSTSPKLKH 443

Query: 444 VVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNS-NVVRGYTIDVFKEALK 503
           V+WPG     PRGWV ++ G+ L+I  P R S+ +FV+Q+  + N+ +G+ IDVF  A+ 
Sbjct: 444 VIWPGETFTKPRGWVFSNNGKELKIGVPLRVSYKEFVSQIRGTENMFKGFCIDVFTAAVN 503

Query: 504 FIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIVDFSQPYTTT 563
            +PY VP +F+P+G+G+ NP+Y  +V+ +    FD  VGD+AIVTNRTKIVDF+QPY  +
Sbjct: 504 LLPYAVPVKFIPYGNGKENPSYTHMVEMITTGNFDGVVGDVAIVTNRTKIVDFTQPYAAS 563

Query: 564 GLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDHFRGPPKRQI 623
           GL++VAP +   S AW FLRPF   MW VT   F+ +GIV+WILEHR ND FRGPPKRQ 
Sbjct: 564 GLVVVAPFKKLNSGAWAFLRPFNRLMWAVTGCCFLFVGIVVWILEHRTNDEFRGPPKRQC 623

Query: 624 ITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILTIQQLPSSIT 683
           +T+  FSFST+F A++E T+S L RLV+++WLF++L+I SSYTASLTSILT+QQL S I 
Sbjct: 624 VTILWFSFSTMFFAHRENTVSTLGRLVLIIWLFVVLIINSSYTASLTSILTVQQLSSPIK 683

Query: 684 GIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVGPKGGGVAAI 743
           GI+ L   + PIGYQVGSFA SYL + L I  SRLV L TPE Y +AL+ GP  GGVAAI
Sbjct: 684 GIESLRERDDPIGYQVGSFAESYLRNELNISESRLVPLGTPEAYAKALKDGPSKGGVAAI 743

Query: 744 IDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLSESGKLQELH 803
           +DE PYVELFLS+   + ++GQ FT+SGWGFAF R+S LA+D+STAIL L+E+G LQ +H
Sbjct: 744 VDERPYVELFLSSNCAYRIVGQEFTKSGWGFAFPRDSPLAIDLSTAILELAENGDLQRIH 803

Query: 804 DSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVRQYIRYKRHR 863
           D W  K  C  E   + E D+LHL SFWGL+L+CG+  + ALF++ + ++RQ  +     
Sbjct: 804 DKWLMKNACTLE-NAELESDRLHLKSFWGLFLICGVACLLALFLYFVQIIRQLYKKPTDD 863

Query: 864 RHFEETTPSPITSHTSCTRSIQSFISFIDEKE----EAIKNLFRGSHGGTQNGDNLQKHS 915
               +   +  +S    TR +Q F+S +DEKE    E+ K    GS   T      +   
Sbjct: 864 AIARDQQQNHDSSSMRSTR-LQRFLSLMDEKEESKHESKKRKIDGSMNDTSGSTRSRGFD 923

BLAST of MC04g0196 vs. NCBI nr
Match: XP_022135996.1 (glutamate receptor 3.7-like [Momordica charantia])

HSP 1 Score: 1837 bits (4759), Expect = 0.0
Identity = 926/926 (100.00%), Postives = 926/926 (100.00%), Query Frame = 0

Query: 1   MRKFAALALRTLVWLLLTGPIWCQKPAVNIGAVFTFNSVIGRAAKPAMEAAISHINADPK 60
           MRKFAALALRTLVWLLLTGPIWCQKPAVNIGAVFTFNSVIGRAAKPAMEAAISHINADPK
Sbjct: 1   MRKFAALALRTLVWLLLTGPIWCQKPAVNIGAVFTFNSVIGRAAKPAMEAAISHINADPK 60

Query: 61  ILDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQV 120
           ILDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQV
Sbjct: 61  ILDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQV 120

Query: 121 SYGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSL 180
           SYGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSL
Sbjct: 121 SYGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSL 180

Query: 181 GDELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKL 240
           GDELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKL
Sbjct: 181 GDELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKL 240

Query: 241 DMMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKRALADSVMNVY 300
           DMMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKRALADSVMNVY
Sbjct: 241 DMMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKRALADSVMNVY 300

Query: 301 GLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRIIM 360
           GLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRIIM
Sbjct: 301 GLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRIIM 360

Query: 361 GTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQED 420
           GTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQED
Sbjct: 361 GTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQED 420

Query: 421 YSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYT 480
           YSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYT
Sbjct: 421 YSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYT 480

Query: 481 IDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIV 540
           IDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIV
Sbjct: 481 IDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIV 540

Query: 541 DFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDH 600
           DFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDH
Sbjct: 541 DFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDH 600

Query: 601 FRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILT 660
           FRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILT
Sbjct: 601 FRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILT 660

Query: 661 IQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVG 720
           IQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVG
Sbjct: 661 IQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVG 720

Query: 721 PKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLS 780
           PKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLS
Sbjct: 721 PKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLS 780

Query: 781 ESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVR 840
           ESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVR
Sbjct: 781 ESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVR 840

Query: 841 QYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFRGSHGGTQNGDN 900
           QYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFRGSHGGTQNGDN
Sbjct: 841 QYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFRGSHGGTQNGDN 900

Query: 901 LQKHSREAVNKIDPEVQMGTSSMNQR 926
           LQKHSREAVNKIDPEVQMGTSSMNQR
Sbjct: 901 LQKHSREAVNKIDPEVQMGTSSMNQR 926

BLAST of MC04g0196 vs. NCBI nr
Match: XP_022988680.1 (glutamate receptor 3.7-like [Cucurbita maxima] >XP_022988681.1 glutamate receptor 3.7-like [Cucurbita maxima])

HSP 1 Score: 1489 bits (3855), Expect = 0.0
Identity = 747/936 (79.81%), Postives = 833/936 (89.00%), Query Frame = 0

Query: 4   FAALAL-RTLVWLLLTGPIWCQKPAV-NIGAVFTFNSVIGRAAKPAMEAAISHINADPKI 63
           FAAL L  TL+WL L+G IWCQK AV NIGAVFTFNSVIGRAAKP M+AAI+ INAD  I
Sbjct: 4   FAALPLLHTLIWLFLSGSIWCQKAAVVNIGAVFTFNSVIGRAAKPGMQAAIADINADSNI 63

Query: 64  LDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVS 123
           L GT++KLLMED+NCS FLG+VGAL VLEKE+VAIIGPQSSVVAHVIS++VNGLQ+PQVS
Sbjct: 64  LSGTKVKLLMEDSNCSDFLGSVGALHVLEKEIVAIIGPQSSVVAHVISELVNGLQIPQVS 123

Query: 124 YGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLG 183
           YGATDP+LS+LQLPFFLRT LSDSYQMAA+ADLI YYGWKEVI I+LDDDYGRNGISSLG
Sbjct: 124 YGATDPTLSTLQLPFFLRTTLSDSYQMAAMADLIDYYGWKEVIVIFLDDDYGRNGISSLG 183

Query: 184 DELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLD 243
           DELQKKMCRISH F LPSLANL++ITE LN SKLLGPRVYVVHVGPDPQLRIF IA KL 
Sbjct: 184 DELQKKMCRISHGFGLPSLANLAKITEILNQSKLLGPRVYVVHVGPDPQLRIFTIAHKLG 243

Query: 244 MMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKR----------- 303
           M++SNYVWFATDWLA TLDSFSPT+ ASLDIL+GVVGLRPHT +S+GK+           
Sbjct: 244 MLSSNYVWFATDWLATTLDSFSPTDLASLDILNGVVGLRPHTQESKGKKDLLNRLSKMQP 303

Query: 304 -ALADSVMNVYGLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVF 363
             LA+SV+NVYGLYAYDSVWVVARAVDKFLKENGNITFSS GKVFG+S SGIQLG+LKVF
Sbjct: 304 KGLANSVLNVYGLYAYDSVWVVARAVDKFLKENGNITFSSTGKVFGTSKSGIQLGRLKVF 363

Query: 364 DGGSDLLRIIMGTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVS 423
           +GGSDLLRIIM TN++GLSGRIQF  DRNIINGSYDVINID K   TVGYW NYSG  +S
Sbjct: 364 NGGSDLLRIIMQTNYSGLSGRIQFGEDRNIINGSYDVINIDQKEIRTVGYWFNYSGFSIS 423

Query: 424 PPEDLTLKQEDYSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQ 483
           PPE LTLKQ+D S L+QK++ VVWPGG ++ P GWVIADAG+PLRIA+P+RESFV+FVTQ
Sbjct: 424 PPEALTLKQKD-SPLDQKLDIVVWPGGNSKIPNGWVIADAGKPLRIAYPRRESFVEFVTQ 483

Query: 484 LNNSNVVRGYTIDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGD 543
           +NN+NVV+GY ID+FK ALK IPY+VPY+FVPFGDG+ NP+YDELVQSVAD+VFDAA+GD
Sbjct: 484 VNNTNVVQGYVIDIFKAALKLIPYEVPYKFVPFGDGQVNPSYDELVQSVADNVFDAAIGD 543

Query: 544 IAIVTNRTKIVDFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIV 603
           IAIVTNRTK+VDFSQPY TTGLIIVAPV+DSKSSAWVFL+PFT EMWCVT  SFVIIGIV
Sbjct: 544 IAIVTNRTKVVDFSQPYITTGLIIVAPVKDSKSSAWVFLKPFTAEMWCVTGFSFVIIGIV 603

Query: 604 IWILEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITS 663
           IW+LEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSR+VMLVWLFLLLVITS
Sbjct: 604 IWMLEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRMVMLVWLFLLLVITS 663

Query: 664 SYTASLTSILTIQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYT 723
           SYTASLTSILTIQ+L S I GIDDL+ASNLPIGYQVGSFAY YLT SLFIPRSRLV LY 
Sbjct: 664 SYTASLTSILTIQKLWSPIRGIDDLVASNLPIGYQVGSFAYDYLTQSLFIPRSRLVKLYN 723

Query: 724 PEDYERALRVGPKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLA 783
           P+DYE+ALR+GPKGGGVAAIIDELPY+ELFLS TKEFGMIGQ FTRSGWGFAFQR SRLA
Sbjct: 724 PDDYEKALRLGPKGGGVAAIIDELPYLELFLSKTKEFGMIGQTFTRSGWGFAFQRRSRLA 783

Query: 784 VDMSTAILRLSESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVA 843
           VDMSTAILRLSE+GKLQE+HD+WFCKLGCPG+R G+++PDQLHLISFWGLYLLCGIIS A
Sbjct: 784 VDMSTAILRLSENGKLQEIHDTWFCKLGCPGQRGGEAQPDQLHLISFWGLYLLCGIISSA 843

Query: 844 ALFVFLLLLVRQYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFR 903
           ALF+FLL ++ QYIRY+R  R  E  TP+PI S+T CT++IQSF+ FIDEK+EAIKN FR
Sbjct: 844 ALFMFLLRIIGQYIRYQRQHRRSEVVTPTPIPSNTGCTQTIQSFMRFIDEKDEAIKNFFR 903

Query: 904 GSH-GGTQNGDNLQKHSREAVNKIDPEVQMGTSSMN 924
            +H  G Q+G+ LQ+HS     K D EVQ+GTSSMN
Sbjct: 904 AAHLRGAQSGEQLQRHSGGTKEKADLEVQLGTSSMN 938

BLAST of MC04g0196 vs. NCBI nr
Match: XP_022930646.1 (glutamate receptor 3.7-like [Cucurbita moschata] >XP_022930647.1 glutamate receptor 3.7-like [Cucurbita moschata])

HSP 1 Score: 1482 bits (3836), Expect = 0.0
Identity = 743/936 (79.38%), Postives = 829/936 (88.57%), Query Frame = 0

Query: 4   FAALAL-RTLVWLLLTGPIWCQKPAV-NIGAVFTFNSVIGRAAKPAMEAAISHINADPKI 63
           F AL L  TL+WL L+G IWCQK  V NIGAVFTFNSVIGRAAKPAMEAAI+ INADP I
Sbjct: 4   FGALPLLHTLIWLFLSGSIWCQKAVVVNIGAVFTFNSVIGRAAKPAMEAAIADINADPNI 63

Query: 64  LDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVS 123
           L GT++KLLMED+NCS FLG+VGAL VLEKE+VAIIGPQSSVVAHVIS++VNGLQ+PQVS
Sbjct: 64  LSGTKVKLLMEDSNCSDFLGSVGALHVLEKEIVAIIGPQSSVVAHVISELVNGLQIPQVS 123

Query: 124 YGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLG 183
           YGATDP+LS+LQLPFFLRT LSDSYQMAA+ADLI YYGWKEVI I+LDDDYGRNGISSLG
Sbjct: 124 YGATDPTLSTLQLPFFLRTTLSDSYQMAAMADLIDYYGWKEVIVIFLDDDYGRNGISSLG 183

Query: 184 DELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLD 243
           DELQKKMCRI+H F LPSLANL++ITE LN SKLLGPRVYVVHVGPDPQLRIF IA KL 
Sbjct: 184 DELQKKMCRIAHGFGLPSLANLTKITEILNQSKLLGPRVYVVHVGPDPQLRIFTIAHKLG 243

Query: 244 MMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKR----------- 303
           M++SNYVWFATDWLA TLDSFSPT+ ASLDIL+GVVGLRPHT +S+GK+           
Sbjct: 244 MLSSNYVWFATDWLATTLDSFSPTDLASLDILNGVVGLRPHTQESKGKKDLWNRLSKMQP 303

Query: 304 -ALADSVMNVYGLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVF 363
             LA+SV+NVYGLYAYDSVWVVARAVDKFLKENGNITFSS GKVFG+S SGIQLG+LKVF
Sbjct: 304 KGLANSVLNVYGLYAYDSVWVVARAVDKFLKENGNITFSSTGKVFGTSKSGIQLGRLKVF 363

Query: 364 DGGSDLLRIIMGTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVS 423
           +GGSDLLRIIM TN++GLSGRIQF  DRNIINGSYDVINID K   TVGYW NYSG  +S
Sbjct: 364 NGGSDLLRIIMQTNYSGLSGRIQFGEDRNIINGSYDVINIDQKEIRTVGYWFNYSGFSIS 423

Query: 424 PPEDLTLKQEDYSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQ 483
           PPE LTLKQ+D S L+QK++ VVWPGG ++ P GWVIADAG+PLRIA+P+R SFV+FVTQ
Sbjct: 424 PPEALTLKQKD-SPLDQKLDIVVWPGGNSKIPNGWVIADAGKPLRIAYPRRASFVEFVTQ 483

Query: 484 LNNSNVVRGYTIDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGD 543
           +NN+N+V+GY ID+FK ALK IPY+VPY+FVPFGDG  NP+YDELVQSVAD VFDAAVGD
Sbjct: 484 VNNTNIVQGYVIDIFKAALKLIPYEVPYKFVPFGDGLVNPSYDELVQSVADSVFDAAVGD 543

Query: 544 IAIVTNRTKIVDFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIV 603
           IAIVTNRTK+VDFSQPY TTGLIIVAPV+DSKSSAWVFL+PFT EMWCVT  SFVIIGIV
Sbjct: 544 IAIVTNRTKVVDFSQPYITTGLIIVAPVKDSKSSAWVFLKPFTAEMWCVTGFSFVIIGIV 603

Query: 604 IWILEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITS 663
           IW+LEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSR+VMLVWLFLLLVITS
Sbjct: 604 IWMLEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRMVMLVWLFLLLVITS 663

Query: 664 SYTASLTSILTIQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYT 723
           SYTASLTSILTIQ+L S I GIDDL+ASN+PIGYQVGSFAY YLT SLFIPRSRLV L+ 
Sbjct: 664 SYTASLTSILTIQKLWSPIRGIDDLVASNVPIGYQVGSFAYDYLTQSLFIPRSRLVELHD 723

Query: 724 PEDYERALRVGPKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLA 783
           P+DYE+ALR+GPKGGGVAAIIDELPY+ELFLS TKEFGMIGQ FTRSGWGFAFQR SRLA
Sbjct: 724 PDDYEKALRLGPKGGGVAAIIDELPYLELFLSKTKEFGMIGQTFTRSGWGFAFQRRSRLA 783

Query: 784 VDMSTAILRLSESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVA 843
           VDMSTAILRLSE+GKLQE+HD+WFCKLGCPG+R G+++PDQLHLISFWGLYLLCGIIS  
Sbjct: 784 VDMSTAILRLSENGKLQEIHDTWFCKLGCPGQRGGEAQPDQLHLISFWGLYLLCGIISST 843

Query: 844 ALFVFLLLLVRQYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFR 903
           ALF+FLL ++ QYIRY+R  RH E  TP+PI S+T CT++IQSF+ FIDEK+EAIKN FR
Sbjct: 844 ALFMFLLRIIGQYIRYQRQHRHSEVVTPTPIPSNTGCTQTIQSFMRFIDEKDEAIKNFFR 903

Query: 904 GSH-GGTQNGDNLQKHSREAVNKIDPEVQMGTSSMN 924
            +H  G Q+G+ LQ+HS     K D EVQ+GTSS N
Sbjct: 904 AAHLRGAQSGEQLQRHSGGTKEKADLEVQLGTSSTN 938

BLAST of MC04g0196 vs. NCBI nr
Match: XP_023530192.1 (glutamate receptor 3.7-like [Cucurbita pepo subsp. pepo] >XP_023530194.1 glutamate receptor 3.7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1482 bits (3836), Expect = 0.0
Identity = 743/936 (79.38%), Postives = 830/936 (88.68%), Query Frame = 0

Query: 4   FAALAL-RTLVWLLLTGPIWCQKPAV-NIGAVFTFNSVIGRAAKPAMEAAISHINADPKI 63
           F AL L  TL+WL L+G IWCQK AV NIGAVFTFNSVIGRAAKPAMEAAI+ INADP I
Sbjct: 4   FGALPLLHTLIWLFLSGSIWCQKAAVVNIGAVFTFNSVIGRAAKPAMEAAIADINADPNI 63

Query: 64  LDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVS 123
           L GT++KLLMED+NCS FLG+VGAL VLEKE+VAIIGPQSSVVAHVIS++VNGLQ+PQVS
Sbjct: 64  LSGTKVKLLMEDSNCSDFLGSVGALHVLEKEIVAIIGPQSSVVAHVISELVNGLQIPQVS 123

Query: 124 YGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLG 183
           YGATDP+LS+LQLPFFLRT LSDSYQMAA+ADLI YYGWKEVI I+LDDDYGRNGISSLG
Sbjct: 124 YGATDPTLSTLQLPFFLRTTLSDSYQMAAMADLIDYYGWKEVIVIFLDDDYGRNGISSLG 183

Query: 184 DELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLD 243
           DELQKKMCRI+H F LPSLANL++ITE LN SKLLGPRVYVVHVGPDPQLRIF IA KL 
Sbjct: 184 DELQKKMCRIAHGFGLPSLANLTKITEILNQSKLLGPRVYVVHVGPDPQLRIFTIAHKLG 243

Query: 244 MMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKR----------- 303
           M++SNYVWFATDWLA TLDSFSPT+ ASLDIL+GVVGLRPHT +S+GK+           
Sbjct: 244 MLSSNYVWFATDWLATTLDSFSPTDLASLDILNGVVGLRPHTQESKGKKDLWNRLSKMQP 303

Query: 304 -ALADSVMNVYGLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVF 363
             LA+S +NVYGLYAYDSVWVVARAVDKFLKENGNITFSS GKVFG+S SGIQLG+LKVF
Sbjct: 304 KGLANSALNVYGLYAYDSVWVVARAVDKFLKENGNITFSSTGKVFGTSKSGIQLGRLKVF 363

Query: 364 DGGSDLLRIIMGTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVS 423
           +GGSDLLRIIM TN++GLSGRIQF  DRNIINGSYDVINID K   TVGYW NYSG  +S
Sbjct: 364 NGGSDLLRIIMQTNYSGLSGRIQFGEDRNIINGSYDVINIDQKEIRTVGYWFNYSGFSIS 423

Query: 424 PPEDLTLKQEDYSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQ 483
           PPE LTLKQ+D S L+QK++ VVWPGG +  P GWVIADAG+PLRIA+P+R SFV+FVTQ
Sbjct: 424 PPEALTLKQKD-SPLDQKLDIVVWPGGNSNIPNGWVIADAGKPLRIAYPRRASFVEFVTQ 483

Query: 484 LNNSNVVRGYTIDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGD 543
           +NN+N+V+GY ID+FK ALK IPY+VPY+FVPFGDG+ NP+YDELVQSVAD+VFDAAVGD
Sbjct: 484 VNNTNIVQGYVIDIFKAALKLIPYEVPYKFVPFGDGQVNPSYDELVQSVADNVFDAAVGD 543

Query: 544 IAIVTNRTKIVDFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIV 603
           IAIVTNRTK+VDFSQPY TTGLIIVAPV+DSKSSAWVFL+PFT EMWCVT  SFVIIGIV
Sbjct: 544 IAIVTNRTKVVDFSQPYITTGLIIVAPVKDSKSSAWVFLKPFTAEMWCVTGFSFVIIGIV 603

Query: 604 IWILEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITS 663
           IW+LEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSR+VMLVWLFLLLVITS
Sbjct: 604 IWMLEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRMVMLVWLFLLLVITS 663

Query: 664 SYTASLTSILTIQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYT 723
           SYTASLTSILTIQ+L S I GIDDL+ASN+PIGYQVGSFAY YLT SLFIPRSRLV L+ 
Sbjct: 664 SYTASLTSILTIQKLWSPIRGIDDLVASNVPIGYQVGSFAYDYLTQSLFIPRSRLVELHD 723

Query: 724 PEDYERALRVGPKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLA 783
           P+DYE+ALR+GPKGGGVAAIIDELPY+ELFLS TKEFGMIGQ FTRSGWGFAFQR SRLA
Sbjct: 724 PDDYEKALRLGPKGGGVAAIIDELPYLELFLSKTKEFGMIGQTFTRSGWGFAFQRRSRLA 783

Query: 784 VDMSTAILRLSESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVA 843
           VDMSTAILRLSE+GKLQE+HD+WFCKLGCPG+R G++EPDQLHLISFWGLYLLCGIIS  
Sbjct: 784 VDMSTAILRLSENGKLQEIHDTWFCKLGCPGQRGGEAEPDQLHLISFWGLYLLCGIISST 843

Query: 844 ALFVFLLLLVRQYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFR 903
           ALF+FLL ++ QYIRY+R  R  E  TP+PI S+T CT++IQSF+ FIDEK+EAIKN FR
Sbjct: 844 ALFMFLLRIIGQYIRYQRQHRRSEVVTPTPIPSNTGCTQTIQSFMRFIDEKDEAIKNFFR 903

Query: 904 GSH-GGTQNGDNLQKHSREAVNKIDPEVQMGTSSMN 924
            +H  G Q+G+ LQ+HS     K + EVQ+GTSSMN
Sbjct: 904 AAHLRGAQSGEQLQRHSGGTKEKANLEVQLGTSSMN 938

BLAST of MC04g0196 vs. NCBI nr
Match: KAG6589169.1 (Glutamate receptor 3.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1469 bits (3802), Expect = 0.0
Identity = 736/927 (79.40%), Postives = 821/927 (88.57%), Query Frame = 0

Query: 4    FAALAL-RTLVWLLLTGPIWCQKPAV-NIGAVFTFNSVIGRAAKPAMEAAISHINADPKI 63
            F AL L  TL+WL L+G IWCQK  V NIGAVFTFNSVIGRAAKPAMEAAI+ INADP I
Sbjct: 94   FGALPLLHTLIWLFLSGSIWCQKAVVVNIGAVFTFNSVIGRAAKPAMEAAIADINADPNI 153

Query: 64   LDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVS 123
            L GT++KLLMED+NCS FLG+VGAL VLEKE+VAIIGPQSSVVAHVIS++VNGLQ+PQVS
Sbjct: 154  LSGTKVKLLMEDSNCSDFLGSVGALHVLEKEIVAIIGPQSSVVAHVISELVNGLQIPQVS 213

Query: 124  YGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLG 183
            YGATDP+LS+LQLPFFLRT LSDSYQMAA+ADLI YYGWKEVI I+LDDDYGRNGISSLG
Sbjct: 214  YGATDPTLSTLQLPFFLRTTLSDSYQMAAMADLIDYYGWKEVIVIFLDDDYGRNGISSLG 273

Query: 184  DELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLD 243
            DELQKKMCRI+H F LPSLANL++ITE LN SKLLGPRVYVVHVGPDPQLRIF IA KL 
Sbjct: 274  DELQKKMCRIAHGFGLPSLANLTKITEILNQSKLLGPRVYVVHVGPDPQLRIFTIAHKLG 333

Query: 244  MMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKR----------- 303
            M++SNYVWFATDWLA TLDSFSPT+ ASLDIL+GVVGLRPHT +S+GK+           
Sbjct: 334  MLSSNYVWFATDWLATTLDSFSPTDLASLDILNGVVGLRPHTQESKGKKDLWNRLSKMQP 393

Query: 304  -ALADSVMNVYGLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVF 363
              LA+SV+NVYGLYAYDSVWVVARAVDKFLKENGNITFSS GKVFG+S SGIQLG+LKVF
Sbjct: 394  KGLANSVLNVYGLYAYDSVWVVARAVDKFLKENGNITFSSTGKVFGTSKSGIQLGRLKVF 453

Query: 364  DGGSDLLRIIMGTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVS 423
            +GGSDLLRIIM TN++GLSGRIQF  DRNIINGSYDVINID K   TVGYW NYSG  +S
Sbjct: 454  NGGSDLLRIIMQTNYSGLSGRIQFGEDRNIINGSYDVINIDQKEIRTVGYWFNYSGFSIS 513

Query: 424  PPEDLTLKQEDYSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQ 483
            PPE LTLKQ+D S L+QK++ VVWPGG ++ P GWVIADAG+PLRIA+P+R SFV+FVTQ
Sbjct: 514  PPEALTLKQKD-SPLDQKLDIVVWPGGNSKIPNGWVIADAGKPLRIAYPRRASFVEFVTQ 573

Query: 484  LNNSNVVRGYTIDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGD 543
            +NN+N+V+GY ID+FK ALK IPY+VPY+FVPFGDG  NP+YDELVQSVAD VFDAAVGD
Sbjct: 574  VNNTNIVQGYVIDIFKAALKLIPYEVPYKFVPFGDGLVNPSYDELVQSVADSVFDAAVGD 633

Query: 544  IAIVTNRTKIVDFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIV 603
            IAIVTNRTK+VDFSQPY TTGLIIVAPV+DSKSSAWVFL+PFT EMWCVT  SFVIIGIV
Sbjct: 634  IAIVTNRTKVVDFSQPYITTGLIIVAPVKDSKSSAWVFLKPFTAEMWCVTGFSFVIIGIV 693

Query: 604  IWILEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITS 663
            IW+LEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSR+VMLVWLFLLLVITS
Sbjct: 694  IWMLEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRMVMLVWLFLLLVITS 753

Query: 664  SYTASLTSILTIQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYT 723
            SYTASLTSILTIQ+L S I GIDDL+ASN+PIGYQVGSFAY YLT SLFIPRSRLV L+ 
Sbjct: 754  SYTASLTSILTIQKLWSPIRGIDDLVASNVPIGYQVGSFAYDYLTQSLFIPRSRLVELHD 813

Query: 724  PEDYERALRVGPKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLA 783
            P+DYE+ALR+GPKGGGVAAIIDELPY+ELFLS TKEFGMIGQ FTRSGWGFAFQR SRLA
Sbjct: 814  PDDYEKALRLGPKGGGVAAIIDELPYLELFLSKTKEFGMIGQTFTRSGWGFAFQRRSRLA 873

Query: 784  VDMSTAILRLSESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVA 843
            VDMSTAILRLSE+GKLQE+HD+WFCKLGCPG+R G+++PDQLHLISFWGLYLLCGIIS  
Sbjct: 874  VDMSTAILRLSENGKLQEIHDTWFCKLGCPGQRGGEAQPDQLHLISFWGLYLLCGIISST 933

Query: 844  ALFVFLLLLVRQYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFR 903
            ALF+FLL ++ QYIRY+R  RH E  TP+PI S+T CT++IQSF+ FIDEK+EAIKN FR
Sbjct: 934  ALFMFLLRIIGQYIRYQRQHRHSEVVTPTPIPSNTGCTQTIQSFMRFIDEKDEAIKNFFR 993

Query: 904  GSH-GGTQNGDNLQKHSREAVNKIDPE 915
             +H  G Q+G+ LQ+HS     K D E
Sbjct: 994  AAHLRGAQSGEQLQRHSGGTKEKADLE 1019

BLAST of MC04g0196 vs. ExPASy TrEMBL
Match: A0A6J1C299 (Glutamate receptor OS=Momordica charantia OX=3673 GN=LOC111007805 PE=3 SV=1)

HSP 1 Score: 1837 bits (4759), Expect = 0.0
Identity = 926/926 (100.00%), Postives = 926/926 (100.00%), Query Frame = 0

Query: 1   MRKFAALALRTLVWLLLTGPIWCQKPAVNIGAVFTFNSVIGRAAKPAMEAAISHINADPK 60
           MRKFAALALRTLVWLLLTGPIWCQKPAVNIGAVFTFNSVIGRAAKPAMEAAISHINADPK
Sbjct: 1   MRKFAALALRTLVWLLLTGPIWCQKPAVNIGAVFTFNSVIGRAAKPAMEAAISHINADPK 60

Query: 61  ILDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQV 120
           ILDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQV
Sbjct: 61  ILDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQV 120

Query: 121 SYGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSL 180
           SYGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSL
Sbjct: 121 SYGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSL 180

Query: 181 GDELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKL 240
           GDELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKL
Sbjct: 181 GDELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKL 240

Query: 241 DMMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKRALADSVMNVY 300
           DMMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKRALADSVMNVY
Sbjct: 241 DMMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKRALADSVMNVY 300

Query: 301 GLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRIIM 360
           GLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRIIM
Sbjct: 301 GLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRIIM 360

Query: 361 GTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQED 420
           GTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQED
Sbjct: 361 GTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQED 420

Query: 421 YSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYT 480
           YSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYT
Sbjct: 421 YSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYT 480

Query: 481 IDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIV 540
           IDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIV
Sbjct: 481 IDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIV 540

Query: 541 DFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDH 600
           DFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDH
Sbjct: 541 DFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDH 600

Query: 601 FRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILT 660
           FRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILT
Sbjct: 601 FRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILT 660

Query: 661 IQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVG 720
           IQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVG
Sbjct: 661 IQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVG 720

Query: 721 PKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLS 780
           PKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLS
Sbjct: 721 PKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLS 780

Query: 781 ESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVR 840
           ESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVR
Sbjct: 781 ESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVR 840

Query: 841 QYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFRGSHGGTQNGDN 900
           QYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFRGSHGGTQNGDN
Sbjct: 841 QYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFRGSHGGTQNGDN 900

Query: 901 LQKHSREAVNKIDPEVQMGTSSMNQR 926
           LQKHSREAVNKIDPEVQMGTSSMNQR
Sbjct: 901 LQKHSREAVNKIDPEVQMGTSSMNQR 926

BLAST of MC04g0196 vs. ExPASy TrEMBL
Match: A0A6J1JHX3 (Glutamate receptor OS=Cucurbita maxima OX=3661 GN=LOC111485937 PE=3 SV=1)

HSP 1 Score: 1489 bits (3855), Expect = 0.0
Identity = 747/936 (79.81%), Postives = 833/936 (89.00%), Query Frame = 0

Query: 4   FAALAL-RTLVWLLLTGPIWCQKPAV-NIGAVFTFNSVIGRAAKPAMEAAISHINADPKI 63
           FAAL L  TL+WL L+G IWCQK AV NIGAVFTFNSVIGRAAKP M+AAI+ INAD  I
Sbjct: 4   FAALPLLHTLIWLFLSGSIWCQKAAVVNIGAVFTFNSVIGRAAKPGMQAAIADINADSNI 63

Query: 64  LDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVS 123
           L GT++KLLMED+NCS FLG+VGAL VLEKE+VAIIGPQSSVVAHVIS++VNGLQ+PQVS
Sbjct: 64  LSGTKVKLLMEDSNCSDFLGSVGALHVLEKEIVAIIGPQSSVVAHVISELVNGLQIPQVS 123

Query: 124 YGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLG 183
           YGATDP+LS+LQLPFFLRT LSDSYQMAA+ADLI YYGWKEVI I+LDDDYGRNGISSLG
Sbjct: 124 YGATDPTLSTLQLPFFLRTTLSDSYQMAAMADLIDYYGWKEVIVIFLDDDYGRNGISSLG 183

Query: 184 DELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLD 243
           DELQKKMCRISH F LPSLANL++ITE LN SKLLGPRVYVVHVGPDPQLRIF IA KL 
Sbjct: 184 DELQKKMCRISHGFGLPSLANLAKITEILNQSKLLGPRVYVVHVGPDPQLRIFTIAHKLG 243

Query: 244 MMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKR----------- 303
           M++SNYVWFATDWLA TLDSFSPT+ ASLDIL+GVVGLRPHT +S+GK+           
Sbjct: 244 MLSSNYVWFATDWLATTLDSFSPTDLASLDILNGVVGLRPHTQESKGKKDLLNRLSKMQP 303

Query: 304 -ALADSVMNVYGLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVF 363
             LA+SV+NVYGLYAYDSVWVVARAVDKFLKENGNITFSS GKVFG+S SGIQLG+LKVF
Sbjct: 304 KGLANSVLNVYGLYAYDSVWVVARAVDKFLKENGNITFSSTGKVFGTSKSGIQLGRLKVF 363

Query: 364 DGGSDLLRIIMGTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVS 423
           +GGSDLLRIIM TN++GLSGRIQF  DRNIINGSYDVINID K   TVGYW NYSG  +S
Sbjct: 364 NGGSDLLRIIMQTNYSGLSGRIQFGEDRNIINGSYDVINIDQKEIRTVGYWFNYSGFSIS 423

Query: 424 PPEDLTLKQEDYSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQ 483
           PPE LTLKQ+D S L+QK++ VVWPGG ++ P GWVIADAG+PLRIA+P+RESFV+FVTQ
Sbjct: 424 PPEALTLKQKD-SPLDQKLDIVVWPGGNSKIPNGWVIADAGKPLRIAYPRRESFVEFVTQ 483

Query: 484 LNNSNVVRGYTIDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGD 543
           +NN+NVV+GY ID+FK ALK IPY+VPY+FVPFGDG+ NP+YDELVQSVAD+VFDAA+GD
Sbjct: 484 VNNTNVVQGYVIDIFKAALKLIPYEVPYKFVPFGDGQVNPSYDELVQSVADNVFDAAIGD 543

Query: 544 IAIVTNRTKIVDFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIV 603
           IAIVTNRTK+VDFSQPY TTGLIIVAPV+DSKSSAWVFL+PFT EMWCVT  SFVIIGIV
Sbjct: 544 IAIVTNRTKVVDFSQPYITTGLIIVAPVKDSKSSAWVFLKPFTAEMWCVTGFSFVIIGIV 603

Query: 604 IWILEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITS 663
           IW+LEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSR+VMLVWLFLLLVITS
Sbjct: 604 IWMLEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRMVMLVWLFLLLVITS 663

Query: 664 SYTASLTSILTIQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYT 723
           SYTASLTSILTIQ+L S I GIDDL+ASNLPIGYQVGSFAY YLT SLFIPRSRLV LY 
Sbjct: 664 SYTASLTSILTIQKLWSPIRGIDDLVASNLPIGYQVGSFAYDYLTQSLFIPRSRLVKLYN 723

Query: 724 PEDYERALRVGPKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLA 783
           P+DYE+ALR+GPKGGGVAAIIDELPY+ELFLS TKEFGMIGQ FTRSGWGFAFQR SRLA
Sbjct: 724 PDDYEKALRLGPKGGGVAAIIDELPYLELFLSKTKEFGMIGQTFTRSGWGFAFQRRSRLA 783

Query: 784 VDMSTAILRLSESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVA 843
           VDMSTAILRLSE+GKLQE+HD+WFCKLGCPG+R G+++PDQLHLISFWGLYLLCGIIS A
Sbjct: 784 VDMSTAILRLSENGKLQEIHDTWFCKLGCPGQRGGEAQPDQLHLISFWGLYLLCGIISSA 843

Query: 844 ALFVFLLLLVRQYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFR 903
           ALF+FLL ++ QYIRY+R  R  E  TP+PI S+T CT++IQSF+ FIDEK+EAIKN FR
Sbjct: 844 ALFMFLLRIIGQYIRYQRQHRRSEVVTPTPIPSNTGCTQTIQSFMRFIDEKDEAIKNFFR 903

Query: 904 GSH-GGTQNGDNLQKHSREAVNKIDPEVQMGTSSMN 924
            +H  G Q+G+ LQ+HS     K D EVQ+GTSSMN
Sbjct: 904 AAHLRGAQSGEQLQRHSGGTKEKADLEVQLGTSSMN 938

BLAST of MC04g0196 vs. ExPASy TrEMBL
Match: A0A6J1ERI4 (Glutamate receptor OS=Cucurbita moschata OX=3662 GN=LOC111437050 PE=3 SV=1)

HSP 1 Score: 1482 bits (3836), Expect = 0.0
Identity = 743/936 (79.38%), Postives = 829/936 (88.57%), Query Frame = 0

Query: 4   FAALAL-RTLVWLLLTGPIWCQKPAV-NIGAVFTFNSVIGRAAKPAMEAAISHINADPKI 63
           F AL L  TL+WL L+G IWCQK  V NIGAVFTFNSVIGRAAKPAMEAAI+ INADP I
Sbjct: 4   FGALPLLHTLIWLFLSGSIWCQKAVVVNIGAVFTFNSVIGRAAKPAMEAAIADINADPNI 63

Query: 64  LDGTQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVS 123
           L GT++KLLMED+NCS FLG+VGAL VLEKE+VAIIGPQSSVVAHVIS++VNGLQ+PQVS
Sbjct: 64  LSGTKVKLLMEDSNCSDFLGSVGALHVLEKEIVAIIGPQSSVVAHVISELVNGLQIPQVS 123

Query: 124 YGATDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLG 183
           YGATDP+LS+LQLPFFLRT LSDSYQMAA+ADLI YYGWKEVI I+LDDDYGRNGISSLG
Sbjct: 124 YGATDPTLSTLQLPFFLRTTLSDSYQMAAMADLIDYYGWKEVIVIFLDDDYGRNGISSLG 183

Query: 184 DELQKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLD 243
           DELQKKMCRI+H F LPSLANL++ITE LN SKLLGPRVYVVHVGPDPQLRIF IA KL 
Sbjct: 184 DELQKKMCRIAHGFGLPSLANLTKITEILNQSKLLGPRVYVVHVGPDPQLRIFTIAHKLG 243

Query: 244 MMTSNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSEGKR----------- 303
           M++SNYVWFATDWLA TLDSFSPT+ ASLDIL+GVVGLRPHT +S+GK+           
Sbjct: 244 MLSSNYVWFATDWLATTLDSFSPTDLASLDILNGVVGLRPHTQESKGKKDLWNRLSKMQP 303

Query: 304 -ALADSVMNVYGLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVF 363
             LA+SV+NVYGLYAYDSVWVVARAVDKFLKENGNITFSS GKVFG+S SGIQLG+LKVF
Sbjct: 304 KGLANSVLNVYGLYAYDSVWVVARAVDKFLKENGNITFSSTGKVFGTSKSGIQLGRLKVF 363

Query: 364 DGGSDLLRIIMGTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVS 423
           +GGSDLLRIIM TN++GLSGRIQF  DRNIINGSYDVINID K   TVGYW NYSG  +S
Sbjct: 364 NGGSDLLRIIMQTNYSGLSGRIQFGEDRNIINGSYDVINIDQKEIRTVGYWFNYSGFSIS 423

Query: 424 PPEDLTLKQEDYSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQ 483
           PPE LTLKQ+D S L+QK++ VVWPGG ++ P GWVIADAG+PLRIA+P+R SFV+FVTQ
Sbjct: 424 PPEALTLKQKD-SPLDQKLDIVVWPGGNSKIPNGWVIADAGKPLRIAYPRRASFVEFVTQ 483

Query: 484 LNNSNVVRGYTIDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGD 543
           +NN+N+V+GY ID+FK ALK IPY+VPY+FVPFGDG  NP+YDELVQSVAD VFDAAVGD
Sbjct: 484 VNNTNIVQGYVIDIFKAALKLIPYEVPYKFVPFGDGLVNPSYDELVQSVADSVFDAAVGD 543

Query: 544 IAIVTNRTKIVDFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIV 603
           IAIVTNRTK+VDFSQPY TTGLIIVAPV+DSKSSAWVFL+PFT EMWCVT  SFVIIGIV
Sbjct: 544 IAIVTNRTKVVDFSQPYITTGLIIVAPVKDSKSSAWVFLKPFTAEMWCVTGFSFVIIGIV 603

Query: 604 IWILEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITS 663
           IW+LEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSR+VMLVWLFLLLVITS
Sbjct: 604 IWMLEHRINDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRMVMLVWLFLLLVITS 663

Query: 664 SYTASLTSILTIQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYT 723
           SYTASLTSILTIQ+L S I GIDDL+ASN+PIGYQVGSFAY YLT SLFIPRSRLV L+ 
Sbjct: 664 SYTASLTSILTIQKLWSPIRGIDDLVASNVPIGYQVGSFAYDYLTQSLFIPRSRLVELHD 723

Query: 724 PEDYERALRVGPKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLA 783
           P+DYE+ALR+GPKGGGVAAIIDELPY+ELFLS TKEFGMIGQ FTRSGWGFAFQR SRLA
Sbjct: 724 PDDYEKALRLGPKGGGVAAIIDELPYLELFLSKTKEFGMIGQTFTRSGWGFAFQRRSRLA 783

Query: 784 VDMSTAILRLSESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVA 843
           VDMSTAILRLSE+GKLQE+HD+WFCKLGCPG+R G+++PDQLHLISFWGLYLLCGIIS  
Sbjct: 784 VDMSTAILRLSENGKLQEIHDTWFCKLGCPGQRGGEAQPDQLHLISFWGLYLLCGIISST 843

Query: 844 ALFVFLLLLVRQYIRYKRHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFR 903
           ALF+FLL ++ QYIRY+R  RH E  TP+PI S+T CT++IQSF+ FIDEK+EAIKN FR
Sbjct: 844 ALFMFLLRIIGQYIRYQRQHRHSEVVTPTPIPSNTGCTQTIQSFMRFIDEKDEAIKNFFR 903

Query: 904 GSH-GGTQNGDNLQKHSREAVNKIDPEVQMGTSSMN 924
            +H  G Q+G+ LQ+HS     K D EVQ+GTSS N
Sbjct: 904 AAHLRGAQSGEQLQRHSGGTKEKADLEVQLGTSSTN 938

BLAST of MC04g0196 vs. ExPASy TrEMBL
Match: A0A1S3C0W0 (Glutamate receptor OS=Cucumis melo OX=3656 GN=LOC103495587 PE=3 SV=1)

HSP 1 Score: 1431 bits (3705), Expect = 0.0
Identity = 720/930 (77.42%), Postives = 813/930 (87.42%), Query Frame = 0

Query: 13  VWLL-LTGPIWCQKPAV-NIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDGTQLKLL 72
           +WL  LT PI+CQ P++ NI AVFTF+SVIGRAAKPAMEAAIS INADP IL+ T+L   
Sbjct: 17  IWLFHLTPPIYCQLPSIINIAAVFTFDSVIGRAAKPAMEAAISDINADPNILNATKLNFF 76

Query: 73  MEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGATDPSLS 132
           MED+NCSGFLG+VGALQVLEKE+VA+IGPQSSVVAHVISQIVNGLQ+PQVSY ATDP+LS
Sbjct: 77  MEDSNCSGFLGSVGALQVLEKEIVALIGPQSSVVAHVISQIVNGLQIPQVSYAATDPTLS 136

Query: 133 SLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDELQKKMCR 192
           +LQLP+FLRT +SDSYQMAA+ADLI YYGWKEVIAI+LDDDYGRNGIS LGDELQKKMCR
Sbjct: 137 TLQLPYFLRTTISDSYQMAAMADLIDYYGWKEVIAIFLDDDYGRNGISFLGDELQKKMCR 196

Query: 193 ISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLDMMTSNYVWF 252
           ISH F LPSL NL++IT+ L+NSKLLGPRVYVVHVGPDPQLRIF IA KL M++SNYVWF
Sbjct: 197 ISHAFPLPSLDNLTKITQILDNSKLLGPRVYVVHVGPDPQLRIFTIAHKLGMLSSNYVWF 256

Query: 253 ATDWLAATLDSFSP-TNSASLDILHGVVGLRPHTPDSEGKR------------ALADSVM 312
           ATDWL+ TLDS SP T  ASLD+L+G+VGLRPHTP+S+GKR             L +S +
Sbjct: 257 ATDWLSTTLDSSSPITIGASLDMLNGIVGLRPHTPESKGKRDLWNRISKMQPKGLTNSAL 316

Query: 313 NVYGLYAYDSVWVVARAVDKFLKENGNI-TFSSQGKVFGSSASGIQLGKLKVFDGGSDLL 372
           NVYGLYAYDSVW+VA+AVDKFLKENG I TFS  GKVFGS+ SGIQLGK+KVFD GSDLL
Sbjct: 317 NVYGLYAYDSVWIVAKAVDKFLKENGTIVTFSPTGKVFGSNESGIQLGKVKVFDRGSDLL 376

Query: 373 RIIMGTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTL 432
           RI+M T++ GLSGRIQF  DR+++NGSYDVINI+ +    VG+WSN S            
Sbjct: 377 RILMQTDYNGLSGRIQFGEDRSVVNGSYDVININQRKMKLVGHWSNDSRF---------- 436

Query: 433 KQEDYSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVV 492
               +S L+QK+E+VVWPGGK E PRGWVIAD+G+PLRIAFP+R SFVDFVTQLNN+N+V
Sbjct: 437 ----HSYLDQKLENVVWPGGKKEIPRGWVIADSGKPLRIAFPRRASFVDFVTQLNNTNIV 496

Query: 493 RGYTIDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNR 552
           +GY ID+FKEALKF+PY+VPY+FVPFGDG+ NP+YDELVQSVA++VFDAAVGDIAI+TNR
Sbjct: 497 QGYVIDIFKEALKFVPYEVPYKFVPFGDGKVNPSYDELVQSVANNVFDAAVGDIAIITNR 556

Query: 553 TKIVDFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHR 612
           TK+VDFSQPYTTTGLIIVAPVEDSKSSAWVFL+PFTVEMWC TA SFV+IGIVIW+LEHR
Sbjct: 557 TKVVDFSQPYTTTGLIIVAPVEDSKSSAWVFLKPFTVEMWCATAGSFVVIGIVIWMLEHR 616

Query: 613 INDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLT 672
           INDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLT
Sbjct: 617 INDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLT 676

Query: 673 SILTIQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERA 732
           SILT+QQL S I GIDDL+ASNLPIGYQVGSFAY YLT SLFIP SRL+ L +PEDYE+A
Sbjct: 677 SILTLQQLWSPIRGIDDLVASNLPIGYQVGSFAYDYLTQSLFIPSSRLLRLDSPEDYEKA 736

Query: 733 LRVGPKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAI 792
           LR+GPKGGGVAAIIDELPY+ELFLSNTKEFG+IGQPFTRSGWGFAFQR SRLAVDMSTAI
Sbjct: 737 LRLGPKGGGVAAIIDELPYLELFLSNTKEFGIIGQPFTRSGWGFAFQRGSRLAVDMSTAI 796

Query: 793 LRLSESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLL 852
           L+LSESGKLQE+HDSWFCKLGCPG R GKSEPDQLHLISFWGLYLLCGIIS+AALF+FLL
Sbjct: 797 LKLSESGKLQEIHDSWFCKLGCPGNRGGKSEPDQLHLISFWGLYLLCGIISLAALFLFLL 856

Query: 853 LLVRQYIRYKRH--RRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFRGSHGG 912
            L+RQYIRY RH  RRH EE TP P+ S++SCT++IQ+FI FIDEKEEAIK+ F  SHG 
Sbjct: 857 RLIRQYIRYLRHHRRRHLEEVTPFPVPSNSSCTQTIQNFIDFIDEKEEAIKSFFGASHG- 916

Query: 913 TQNGDNLQKHSREAVNKIDPEVQMGTSSMN 924
           +QNG+ L  HS+ A  K D E+Q+GT  MN
Sbjct: 917 SQNGNQLHNHSQNAKEKADSEIQIGTMGMN 931

BLAST of MC04g0196 vs. ExPASy TrEMBL
Match: A0A0A0K1M2 (Glutamate receptor OS=Cucumis sativus OX=3659 GN=Csa_7G044210 PE=3 SV=1)

HSP 1 Score: 1417 bits (3667), Expect = 0.0
Identity = 720/929 (77.50%), Postives = 806/929 (86.76%), Query Frame = 0

Query: 13  VWLL-LTGPIWCQKPAV-NIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDGTQLKLL 72
           +WL  LT PI+CQ P++ NI AVFTF+SVIGRAAKPAMEAAI  INADP IL+ T+LK  
Sbjct: 17  IWLFHLTPPIYCQIPSILNIAAVFTFDSVIGRAAKPAMEAAIYDINADPNILNATKLKFF 76

Query: 73  MEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGATDPSLS 132
           ME++NCSGFLG+V ALQVLEKE+VA+IGPQSSVVAHVISQIVNGLQ+P VSY ATDP+LS
Sbjct: 77  MENSNCSGFLGSVQALQVLEKEIVAMIGPQSSVVAHVISQIVNGLQIPLVSYAATDPTLS 136

Query: 133 SLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDELQKKMCR 192
           +LQLPFFLRT +SDSYQMAA+ADLI YYGWKEVI I+LDDDYGRNGIS LGDELQKKMCR
Sbjct: 137 TLQLPFFLRTTISDSYQMAAMADLIDYYGWKEVIVIFLDDDYGRNGISFLGDELQKKMCR 196

Query: 193 ISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLDMMTSNYVWF 252
           ISH F LPSL NLS+IT+ LNNSKLLGPRVYVVHVGPDPQLRIF IA KL M++SNYVWF
Sbjct: 197 ISHAFPLPSLDNLSKITQILNNSKLLGPRVYVVHVGPDPQLRIFTIAHKLGMLSSNYVWF 256

Query: 253 ATDWLAATLDSFSP-TNSASLDILHGVVGLRPHTPDSEGKRALAD------------SVM 312
           ATDWL+ TLDS SP TN ASLD+L+GVVGLRPHTP+S+GKR L D            S +
Sbjct: 257 ATDWLSTTLDSSSPITNGASLDMLNGVVGLRPHTPESKGKRDLWDRLRKMQPKGLTNSAL 316

Query: 313 NVYGLYAYDSVWVVARAVDKFLKENGNI-TFSSQGKVFGSSASGIQLGKLKVFDGGSDLL 372
           NVYGLYAYDSVWVVA+AVDKFLKENGNI TFS  GKV GS+ SGIQLG +KVFD GSDLL
Sbjct: 317 NVYGLYAYDSVWVVAKAVDKFLKENGNIITFSPTGKVLGSNESGIQLGNVKVFDRGSDLL 376

Query: 373 RIIMGTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTL 432
           +I+M T++ GLSGRIQF  DR+++NGSYDVINI+ +  + VG+WSN          DL  
Sbjct: 377 KILMQTDYNGLSGRIQFGEDRSVVNGSYDVININQRKMNLVGHWSN----------DLRF 436

Query: 433 KQEDYSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVV 492
               +  L+QK+E VVWPGGK E PRGWVIAD+G+PLRIAFP+R SFVDFVTQLNN+N+V
Sbjct: 437 ----HPNLDQKLEKVVWPGGKEEIPRGWVIADSGKPLRIAFPRRASFVDFVTQLNNTNIV 496

Query: 493 RGYTIDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNR 552
           RGY ID+FKEALKF+PY+VPY+FVPFGDG+ NP+YDELVQSVA++VFDAAVGDIAIVTNR
Sbjct: 497 RGYVIDIFKEALKFVPYEVPYKFVPFGDGKVNPSYDELVQSVANNVFDAAVGDIAIVTNR 556

Query: 553 TKIVDFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHR 612
           TK+VDFSQPYTTTGLIIVAPVEDSKSSAWVFL+PFTVEMWC TA SFV+IGIVIW+LEHR
Sbjct: 557 TKVVDFSQPYTTTGLIIVAPVEDSKSSAWVFLKPFTVEMWCATAGSFVVIGIVIWMLEHR 616

Query: 613 INDHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLT 672
           INDHFRGPPKRQIITMCLFS STLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLT
Sbjct: 617 INDHFRGPPKRQIITMCLFSISTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLT 676

Query: 673 SILTIQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERA 732
           SILT+QQL S I GIDDL+ASNLPIGYQVGSFAY YLT SLFIP SRL  L + EDYE+A
Sbjct: 677 SILTLQQLWSPIRGIDDLVASNLPIGYQVGSFAYDYLTQSLFIPSSRLQRLNSSEDYEKA 736

Query: 733 LRVGPKGGGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAI 792
           LR+GPKGGGVAAIIDELPY+ELFLS TKEFG+IGQPFTRSGWGFAFQR SRLAVDMSTAI
Sbjct: 737 LRLGPKGGGVAAIIDELPYLELFLSKTKEFGIIGQPFTRSGWGFAFQRGSRLAVDMSTAI 796

Query: 793 LRLSESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLL 852
           L+LSESGKLQE+HDSWFCKLGCPG R GKSEPDQLHLISFWGLYLLCGIISVAALF+FLL
Sbjct: 797 LKLSESGKLQEIHDSWFCKLGCPGNRGGKSEPDQLHLISFWGLYLLCGIISVAALFLFLL 856

Query: 853 LLVRQYIRYKRH--RRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFRGSHGG 912
            L+RQYIRY RH  RRH EE TP P+ S+TSCT+ IQ+FI+FIDEKEEAIK+ F  SHG 
Sbjct: 857 RLIRQYIRYIRHHRRRHSEEVTPFPVPSNTSCTQRIQNFINFIDEKEEAIKSFFGASHGA 916

Query: 913 TQNGDNLQKHSREAVNKIDPEVQMGTSSM 923
            QNG+ L  HS++A  K D E+Q+G ++M
Sbjct: 917 -QNGNQLHNHSQKAKEKADSEIQIGPTTM 930

BLAST of MC04g0196 vs. TAIR 10
Match: AT2G32400.1 (glutamate receptor 5 )

HSP 1 Score: 968.8 bits (2503), Expect = 3.2e-282
Identity = 490/901 (54.38%), Postives = 649/901 (72.03%), Query Frame = 0

Query: 6   ALALRTLVWLLLTGPIWCQKP-AVNIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDG 65
           ++A+  L+ ++L  P+ CQ+P  VNIGAVF F+SVIGRAAK A+EAA+S +N D   L  
Sbjct: 8   SVAITALIVVILVVPMDCQRPQLVNIGAVFAFDSVIGRAAKVALEAAVSDVNNDKSFLKE 67

Query: 66  TQLKLLMEDANCSGFLGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGA 125
           T+L+LLMED+ C+ F G+ GA ++LEKEVVA+IGP SS VAH IS I  GL  P VS+ A
Sbjct: 68  TELRLLMEDSACNVFRGSFGAFELLEKEVVAMIGPISSSVAHTISDIAKGLHFPLVSFAA 127

Query: 126 TDPSLSSLQLPFFLRTALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDEL 185
           TDP+LS+LQ PFFLRT  +D++QM+A+ DLI +YGWKEVI++Y DD+ GRNG+S+L DEL
Sbjct: 128 TDPTLSALQFPFFLRTTPNDAHQMSALVDLINFYGWKEVISVYSDDELGRNGVSALDDEL 187

Query: 186 QKKMCRISHQFALPSLANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLDMMT 245
            KK  RIS++  L   ++   +T ALN SK +GPRVY++H GPDP LRIF IAQKL MMT
Sbjct: 188 YKKRSRISYKVPLSVHSDEKFLTNALNKSKSIGPRVYILHFGPDPLLRIFDIAQKLQMMT 247

Query: 246 SNYVWFATDWLAATLDSFSPTNSASLDILHGVVGLRPHTPDSE-----GKRALADSVMNV 305
             YVW ATDWL+ TLDS S  +  +L  L GVVGLR H P+S        +  ++  MN 
Sbjct: 248 HEYVWLATDWLSVTLDSLS--DKGTLKRLEGVVGLRQHIPESVKMEHFTHKLQSNRSMNA 307

Query: 306 YGLYAYDSVWVVARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRII 365
           Y L+AYD+VW++A  +++ L E  NITFS   K+  +  + + L K+K F+ G  LL  +
Sbjct: 308 YALHAYDTVWMIAHGIEELLNEGINITFSYSEKLLHARGTKLHLEKIKFFNSGELLLEKL 367

Query: 366 MGTNFTGLSGRIQFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQE 425
           +  NFTG++G++QF + RN+I   Y++IN++    HTVG+WS   G  V  P+    +++
Sbjct: 368 LKVNFTGIAGQVQFGSGRNVIGCDYEIINVNKTDVHTVGFWSKNGGFSVVAPKTRHSQKK 427

Query: 426 DYSRLNQKIESVVWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVT-QLNNSNVVRG 485
                ++K+  + WPGG  E PRGWVIAD+  PL+I  P+R SFV+FVT + N+S+ ++G
Sbjct: 428 TSFVSDEKLGDITWPGGGREKPRGWVIADSADPLKIVVPRRVSFVEFVTEEKNSSHRIQG 487

Query: 486 YTIDVFKEALKFIPYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTK 545
           + IDVF EALKF+PY VPY F PFG+G ++PNY+ L+Q V D V+DAAVGDIAIV +R+K
Sbjct: 488 FCIDVFIEALKFVPYSVPYIFEPFGNGHSSPNYNHLIQMVTDGVYDAAVGDIAIVPSRSK 547

Query: 546 IVDFSQPYTTTGLIIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRIN 605
           +VDFSQPY +TGL++V P  D  ++ W+FLRPFT  +WCV   SF++I +VIWILEHRIN
Sbjct: 548 LVDFSQPYASTGLVVVIPANDD-NATWIFLRPFTSRLWCVVLVSFLVIAVVIWILEHRIN 607

Query: 606 DHFRGPPKRQIITMCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSI 665
           + FRGPP+RQ+ TM LFSFSTLFK NQE TIS L+RLVM+VWLFLL+V+T+SYTA+LTSI
Sbjct: 608 EDFRGPPRRQLSTMLLFSFSTLFKRNQEDTISNLARLVMIVWLFLLMVLTASYTANLTSI 667

Query: 666 LTIQQLPSSITGIDDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALR 725
           LT+QQLPS+ITGID L AS +PIGYQ G+F   YLT SL + RSRLV L + E+YE+AL+
Sbjct: 668 LTVQQLPSAITGIDSLRASEVPIGYQAGTFTLEYLTYSLGMARSRLVPLDSTEEYEKALK 727

Query: 726 VGPKG-GGVAAIIDELPYVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAIL 785
           +GP   GGVAAI+DELPY+ELFL+    F ++G+PF   GWGFAF+R+S LA+DMSTAIL
Sbjct: 728 LGPTNWGGVAAIVDELPYIELFLAERTGFKIVGEPFMHRGWGFAFKRDSPLAIDMSTAIL 787

Query: 786 RLSESGKLQELHDSWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLL 845
           +LSE+ KLQE+   W CK  C G+     EP+QLHL SF GLYL+C  I+V+A  VF+L 
Sbjct: 788 KLSETRKLQEIRKKWLCKTNCAGKSNWNPEPNQLHLKSFKGLYLVCIAITVSAFLVFVLR 847

Query: 846 LVRQYIRYKRHRRHFEETTPSPITSHTSCTRS-IQSFISFIDEKEEAIKNLFRGSHGGTQ 898
           ++RQ++RY+R  R       S   S T   R  +  F+ F+DEKEEAIK +FR S     
Sbjct: 848 MIRQFVRYRRMERTSSMPRASWSASPTLRLRELVFDFVEFVDEKEEAIKRMFRRSDDSNN 905

BLAST of MC04g0196 vs. TAIR 10
Match: AT1G05200.1 (glutamate receptor 3.4 )

HSP 1 Score: 877.9 bits (2267), Expect = 7.4e-255
Identity = 444/883 (50.28%), Postives = 609/883 (68.97%), Query Frame = 0

Query: 20  PIWCQKPAVNIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDGTQLKLLMEDANCSGF 79
           P+  +  +VN+GA+FT++S IGRAAKPA++AA+  +NAD  +L G +L ++ +D+NCSGF
Sbjct: 53  PLRQRPSSVNVGALFTYDSFIGRAAKPAVKAAMDDVNADQSVLKGIKLNIIFQDSNCSGF 112

Query: 80  LGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGATDPSLSSLQLPFFLR 139
           +GT+GALQ++E +VVA IGPQSS +AH+IS + N L VP +S+GATDP+LSSLQ P+FLR
Sbjct: 113 IGTMGALQLMENKVVAAIGPQSSGIAHMISYVANELHVPLLSFGATDPTLSSLQFPYFLR 172

Query: 140 TALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDELQKKMCRISHQFALPS 199
           T  +D +QM A+AD + Y GW++VIAI++DD+ GRNGIS LGD L KK  RIS++ A+  
Sbjct: 173 TTQNDYFQMHAIADFLSYSGWRQVIAIFVDDECGRNGISVLGDVLAKKRSRISYKAAITP 232

Query: 200 LANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLDMMTSNYVWFATDWLAATL 259
            A+ S I + L +  L+  RV+VVHV PD  L +F +A+ L MM S YVW ATDWL   +
Sbjct: 233 GADSSSIRDLLVSVNLMESRVFVVHVNPDSGLNVFSVAKSLGMMASGYVWIATDWLPTAM 292

Query: 260 DSFSPTNSASLDILHGVVGLRPHTPDSEGKRAL--------ADSVMNVYGLYAYDSVWVV 319
           DS    +S ++D+L GVV  R +T +S  KR           +   N Y +YAYDSVW+V
Sbjct: 293 DSMEHVDSDTMDLLQGVVAFRHYTIESSVKRQFMARWKNLRPNDGFNSYAMYAYDSVWLV 352

Query: 320 ARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRIIMGTNFTGLSGRI 379
           ARA+D F +EN NITFS+   +  ++ S IQL  L VF+ G   ++II+G N TG++G I
Sbjct: 353 ARALDVFFRENNNITFSNDPNLHKTNGSTIQLSALSVFNEGEKFMKIILGMNHTGVTGPI 412

Query: 380 QFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQEDYSRLNQKIESV 439
           QFD+DRN +N +Y+V+N++     TVGYWSN+SGL V  PE L  +  + S  NQ+++ +
Sbjct: 413 QFDSDRNRVNPAYEVLNLEGTAPRTVGYWSNHSGLSVVHPETLYSRPPNTSTANQRLKGI 472

Query: 440 VWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYTIDVFKEALKFI 499
           ++PG  T+ PRGWV  + G+PLRI  P R S+ D+V++  N   VRGY IDVF+ A++ +
Sbjct: 473 IYPGEVTKPPRGWVFPNNGKPLRIGVPNRVSYTDYVSKDKNPPGVRGYCIDVFEAAIELL 532

Query: 500 PYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIVDFSQPYTTTGL 559
           PY VP  ++ +GDG+ NP+YD LV  V    FD AVGDI IVTNRT+ VDF+QP+  +GL
Sbjct: 533 PYPVPRTYILYGDGKRNPSYDNLVNEVVADNFDVAVGDITIVTNRTRYVDFTQPFIESGL 592

Query: 560 IIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDHFRGPPKRQIIT 619
           ++VAPV+++KSS W FL+PFT+EMW VT   F+ +G ++WILEHR N  FRGPP+RQ+IT
Sbjct: 593 VVVAPVKEAKSSPWSFLKPFTIEMWAVTGGFFLFVGAMVWILEHRFNQEFRGPPRRQLIT 652

Query: 620 MCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILTIQQLPSSITGI 679
           +  FSFST+F +++E T+S L R V+++WLF++L+I SSYTASLTSILTI+QL S I GI
Sbjct: 653 IFWFSFSTMFFSHRENTVSSLGRFVLIIWLFVVLIINSSYTASLTSILTIRQLTSRIEGI 712

Query: 680 DDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVGPKGGGVAAIID 739
           D L+ SN PIG Q G+FA +YL + L I  SR+V L   E Y  AL+ GP  GGVAAI+D
Sbjct: 713 DSLVTSNEPIGVQDGTFARNYLINELNILPSRIVPLKDEEQYLSALQRGPNAGGVAAIVD 772

Query: 740 ELPYVELFLSNTK-EFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLSESGKLQELHD 799
           ELPY+E+ L+N+  +F  +GQ FTR+GWGFAFQR+S LAVDMSTAIL+LSE G+L+++H 
Sbjct: 773 ELPYIEVLLTNSNCKFRTVGQEFTRTGWGFAFQRDSPLAVDMSTAILQLSEEGELEKIHR 832

Query: 800 SWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVRQYIRY----K 859
            W         +   SE  QL L SFWGL+L+CGI    AL VF   +  QY R      
Sbjct: 833 KWLNYKHECSMQISNSEDSQLSLKSFWGLFLICGITCFMALTVFFWRVFWQYQRLLPESA 892

Query: 860 RHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFR 890
              R  E + PS  +   S   S +  I  +D++E  IK + +
Sbjct: 893 DEERAGEVSEPSR-SGRGSRAPSFKELIKVVDKREAEIKEILK 934

BLAST of MC04g0196 vs. TAIR 10
Match: AT1G05200.2 (glutamate receptor 3.4 )

HSP 1 Score: 877.9 bits (2267), Expect = 7.4e-255
Identity = 444/883 (50.28%), Postives = 609/883 (68.97%), Query Frame = 0

Query: 20  PIWCQKPAVNIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDGTQLKLLMEDANCSGF 79
           P+  +  +VN+GA+FT++S IGRAAKPA++AA+  +NAD  +L G +L ++ +D+NCSGF
Sbjct: 53  PLRQRPSSVNVGALFTYDSFIGRAAKPAVKAAMDDVNADQSVLKGIKLNIIFQDSNCSGF 112

Query: 80  LGTVGALQVLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGATDPSLSSLQLPFFLR 139
           +GT+GALQ++E +VVA IGPQSS +AH+IS + N L VP +S+GATDP+LSSLQ P+FLR
Sbjct: 113 IGTMGALQLMENKVVAAIGPQSSGIAHMISYVANELHVPLLSFGATDPTLSSLQFPYFLR 172

Query: 140 TALSDSYQMAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDELQKKMCRISHQFALPS 199
           T  +D +QM A+AD + Y GW++VIAI++DD+ GRNGIS LGD L KK  RIS++ A+  
Sbjct: 173 TTQNDYFQMHAIADFLSYSGWRQVIAIFVDDECGRNGISVLGDVLAKKRSRISYKAAITP 232

Query: 200 LANLSQITEALNNSKLLGPRVYVVHVGPDPQLRIFKIAQKLDMMTSNYVWFATDWLAATL 259
            A+ S I + L +  L+  RV+VVHV PD  L +F +A+ L MM S YVW ATDWL   +
Sbjct: 233 GADSSSIRDLLVSVNLMESRVFVVHVNPDSGLNVFSVAKSLGMMASGYVWIATDWLPTAM 292

Query: 260 DSFSPTNSASLDILHGVVGLRPHTPDSEGKRAL--------ADSVMNVYGLYAYDSVWVV 319
           DS    +S ++D+L GVV  R +T +S  KR           +   N Y +YAYDSVW+V
Sbjct: 293 DSMEHVDSDTMDLLQGVVAFRHYTIESSVKRQFMARWKNLRPNDGFNSYAMYAYDSVWLV 352

Query: 320 ARAVDKFLKENGNITFSSQGKVFGSSASGIQLGKLKVFDGGSDLLRIIMGTNFTGLSGRI 379
           ARA+D F +EN NITFS+   +  ++ S IQL  L VF+ G   ++II+G N TG++G I
Sbjct: 353 ARALDVFFRENNNITFSNDPNLHKTNGSTIQLSALSVFNEGEKFMKIILGMNHTGVTGPI 412

Query: 380 QFDADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQEDYSRLNQKIESV 439
           QFD+DRN +N +Y+V+N++     TVGYWSN+SGL V  PE L  +  + S  NQ+++ +
Sbjct: 413 QFDSDRNRVNPAYEVLNLEGTAPRTVGYWSNHSGLSVVHPETLYSRPPNTSTANQRLKGI 472

Query: 440 VWPGGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYTIDVFKEALKFI 499
           ++PG  T+ PRGWV  + G+PLRI  P R S+ D+V++  N   VRGY IDVF+ A++ +
Sbjct: 473 IYPGEVTKPPRGWVFPNNGKPLRIGVPNRVSYTDYVSKDKNPPGVRGYCIDVFEAAIELL 532

Query: 500 PYDVPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIVDFSQPYTTTGL 559
           PY VP  ++ +GDG+ NP+YD LV  V    FD AVGDI IVTNRT+ VDF+QP+  +GL
Sbjct: 533 PYPVPRTYILYGDGKRNPSYDNLVNEVVADNFDVAVGDITIVTNRTRYVDFTQPFIESGL 592

Query: 560 IIVAPVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDHFRGPPKRQIIT 619
           ++VAPV+++KSS W FL+PFT+EMW VT   F+ +G ++WILEHR N  FRGPP+RQ+IT
Sbjct: 593 VVVAPVKEAKSSPWSFLKPFTIEMWAVTGGFFLFVGAMVWILEHRFNQEFRGPPRRQLIT 652

Query: 620 MCLFSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILTIQQLPSSITGI 679
           +  FSFST+F +++E T+S L R V+++WLF++L+I SSYTASLTSILTI+QL S I GI
Sbjct: 653 IFWFSFSTMFFSHRENTVSSLGRFVLIIWLFVVLIINSSYTASLTSILTIRQLTSRIEGI 712

Query: 680 DDLIASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVGPKGGGVAAIID 739
           D L+ SN PIG Q G+FA +YL + L I  SR+V L   E Y  AL+ GP  GGVAAI+D
Sbjct: 713 DSLVTSNEPIGVQDGTFARNYLINELNILPSRIVPLKDEEQYLSALQRGPNAGGVAAIVD 772

Query: 740 ELPYVELFLSNTK-EFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLSESGKLQELHD 799
           ELPY+E+ L+N+  +F  +GQ FTR+GWGFAFQR+S LAVDMSTAIL+LSE G+L+++H 
Sbjct: 773 ELPYIEVLLTNSNCKFRTVGQEFTRTGWGFAFQRDSPLAVDMSTAILQLSEEGELEKIHR 832

Query: 800 SWFCKLGCPGERRGKSEPDQLHLISFWGLYLLCGIISVAALFVFLLLLVRQYIRY----K 859
            W         +   SE  QL L SFWGL+L+CGI    AL VF   +  QY R      
Sbjct: 833 KWLNYKHECSMQISNSEDSQLSLKSFWGLFLICGITCFMALTVFFWRVFWQYQRLLPESA 892

Query: 860 RHRRHFEETTPSPITSHTSCTRSIQSFISFIDEKEEAIKNLFR 890
              R  E + PS  +   S   S +  I  +D++E  IK + +
Sbjct: 893 DEERAGEVSEPSR-SGRGSRAPSFKELIKVVDKREAEIKEILK 934

BLAST of MC04g0196 vs. TAIR 10
Match: AT4G35290.2 (glutamate receptor 2 )

HSP 1 Score: 866.7 bits (2238), Expect = 1.7e-251
Identity = 448/873 (51.32%), Postives = 606/873 (69.42%), Query Frame = 0

Query: 28  VNIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDGTQLKLLMEDANCSGFLGTVGALQ 87
           V++GA+F+  ++ G     AM+AA   +N+DP  L G++L++   DA  +GFL  +GALQ
Sbjct: 30  VDVGAIFSLGTLQGEVTNIAMKAAEEDVNSDPSFLGGSKLRITTYDAKRNGFLTIMGALQ 89

Query: 88  VLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGATDPSLSSLQLPFFLRTALSDSYQ 147
            +E + VAIIGPQ+S++AHV+S + N L VP +S+ A DPSLS+LQ PFF++TA SD + 
Sbjct: 90  FMETDAVAIIGPQTSIMAHVLSHLANELSVPMLSFTALDPSLSALQFPFFVQTAPSDLFL 149

Query: 148 MAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDELQKKMCRISHQFALPSLANLSQIT 207
           M A+A++I YYGW EVIA+Y DDD  RNGI++LGDEL+ + C+IS++  LP    ++   
Sbjct: 150 MRAIAEMISYYGWSEVIALYNDDDNSRNGITALGDELEGRRCKISYKAVLPLDVVITSPR 209

Query: 208 EALNN-SKLLG--PRVYVVHVGPDPQLRIFKIAQKLDMMTSNYVWFATDWLAATLDSFSP 267
           E +N   K+ G   RV +V+  P    +IF+ AQKL MM   YVW AT WL + LDS +P
Sbjct: 210 EIINELVKIQGMESRVIIVNTFPKTGKKIFEEAQKLGMMEKGYVWIATTWLTSLLDSVNP 269

Query: 268 TNSASLDILHGVVGLRPHTPDSEGKR-------ALADSV--MNVYGLYAYDSVWVVARAV 327
             + + + L GV+ LR HTP+S+ K+        L++    +NVYGLYAYD+VW++ARAV
Sbjct: 270 LPAKTAESLRGVLTLRIHTPNSKKKKDFVARWNKLSNGTVGLNVYGLYAYDTVWIIARAV 329

Query: 328 DKFLKENGNITFSSQGKVFGSSASG-IQLGKLKVFDGGSDLLRIIMGTNFTGLSGRIQFD 387
            + L    NI+FSS  K+      G + LG L +FD GS  L  I+ TN TG++G+IQF 
Sbjct: 330 KRLLDSRANISFSSDPKLTSMKGGGSLNLGALSIFDQGSQFLDYIVNTNMTGVTGQIQFL 389

Query: 388 ADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQEDYSRLNQKIESVVWP 447
            DR++I  SYD+IN+   GF  +GYWSN+SGL + PPE L  K  + S  NQ + +V WP
Sbjct: 390 PDRSMIQPSYDIINVVDDGFRQIGYWSNHSGLSIIPPESLYKKLSNRSSSNQHLNNVTWP 449

Query: 448 GGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYTIDVFKEALKFIPYD 507
           GG +ETPRGWV  + GR LRI  P R SF +FV++L+ SN V+GY IDVF+ A+K I Y 
Sbjct: 450 GGTSETPRGWVFPNNGRRLRIGVPDRASFKEFVSRLDGSNKVQGYAIDVFEAAVKLISYP 509

Query: 508 VPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIVDFSQPYTTTGLIIV 567
           VP+EFV FGDG  NPN++E V +V   VFDA VGDIAIVT RT+IVDF+QPY  +GL++V
Sbjct: 510 VPHEFVLFGDGLKNPNFNEFVNNVTIGVFDAVVGDIAIVTKRTRIVDFTQPYIESGLVVV 569

Query: 568 APVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDHFRGPPKRQIITMCL 627
           APV     + W FLRPFT  MW VTAA F+I+G VIWILEHRIND FRGPP++QI+T+  
Sbjct: 570 APVTKLNDTPWAFLRPFTPPMWAVTAAFFLIVGSVIWILEHRINDEFRGPPRKQIVTILW 629

Query: 628 FSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILTIQQLPSSITGIDDL 687
           FSFST+F +++E T+S L R V+L+WLF++L+ITSSYTASLTSILT+QQL S I G+D L
Sbjct: 630 FSFSTMFFSHRENTVSTLGRAVLLIWLFVVLIITSSYTASLTSILTVQQLNSPIRGVDTL 689

Query: 688 IASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVGPKGGGVAAIIDELP 747
           I+S+  +G+QVGS+A +Y+ D L I RSRLV L +P++Y  AL    + G VAAI+DE P
Sbjct: 690 ISSSGRVGFQVGSYAENYMIDELNIARSRLVPLGSPKEYAAAL----QNGTVAAIVDERP 749

Query: 748 YVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLSESGKLQELHDSWFC 807
           YV+LFLS    F + GQ FTRSGWGFAF R+S LA+DMSTAIL LSE+G+LQ++HD W  
Sbjct: 750 YVDLFLSEFCGFAIRGQEFTRSGWGFAFPRDSPLAIDMSTAILGLSETGQLQKIHDKWLS 809

Query: 808 KLGCPGERRGKSEPD--QLHLISFWGLYLLCGIISVAALFVFLLLLVRQYIRYKRHRRHF 867
           +  C       S+ D  QL L SFWGL+L+CGI    ALF++   +VR + R+ ++    
Sbjct: 810 RSNCSNLNGSVSDEDSEQLKLRSFWGLFLVCGISCFIALFIYFFKIVRDFFRHGKYDE-- 869

Query: 868 EETTPSPITSHTSCTRSIQSFISFIDEKEEAIK 886
           E T PSP    +S ++S+Q+F+++ DEKE+  K
Sbjct: 870 EATVPSP---ESSRSKSLQTFLAYFDEKEDESK 893

BLAST of MC04g0196 vs. TAIR 10
Match: AT4G35290.1 (glutamate receptor 2 )

HSP 1 Score: 866.7 bits (2238), Expect = 1.7e-251
Identity = 448/873 (51.32%), Postives = 606/873 (69.42%), Query Frame = 0

Query: 28  VNIGAVFTFNSVIGRAAKPAMEAAISHINADPKILDGTQLKLLMEDANCSGFLGTVGALQ 87
           V++GA+F+  ++ G     AM+AA   +N+DP  L G++L++   DA  +GFL  +GALQ
Sbjct: 30  VDVGAIFSLGTLQGEVTNIAMKAAEEDVNSDPSFLGGSKLRITTYDAKRNGFLTIMGALQ 89

Query: 88  VLEKEVVAIIGPQSSVVAHVISQIVNGLQVPQVSYGATDPSLSSLQLPFFLRTALSDSYQ 147
            +E + VAIIGPQ+S++AHV+S + N L VP +S+ A DPSLS+LQ PFF++TA SD + 
Sbjct: 90  FMETDAVAIIGPQTSIMAHVLSHLANELSVPMLSFTALDPSLSALQFPFFVQTAPSDLFL 149

Query: 148 MAAVADLIVYYGWKEVIAIYLDDDYGRNGISSLGDELQKKMCRISHQFALPSLANLSQIT 207
           M A+A++I YYGW EVIA+Y DDD  RNGI++LGDEL+ + C+IS++  LP    ++   
Sbjct: 150 MRAIAEMISYYGWSEVIALYNDDDNSRNGITALGDELEGRRCKISYKAVLPLDVVITSPR 209

Query: 208 EALNN-SKLLG--PRVYVVHVGPDPQLRIFKIAQKLDMMTSNYVWFATDWLAATLDSFSP 267
           E +N   K+ G   RV +V+  P    +IF+ AQKL MM   YVW AT WL + LDS +P
Sbjct: 210 EIINELVKIQGMESRVIIVNTFPKTGKKIFEEAQKLGMMEKGYVWIATTWLTSLLDSVNP 269

Query: 268 TNSASLDILHGVVGLRPHTPDSEGKR-------ALADSV--MNVYGLYAYDSVWVVARAV 327
             + + + L GV+ LR HTP+S+ K+        L++    +NVYGLYAYD+VW++ARAV
Sbjct: 270 LPAKTAESLRGVLTLRIHTPNSKKKKDFVARWNKLSNGTVGLNVYGLYAYDTVWIIARAV 329

Query: 328 DKFLKENGNITFSSQGKVFGSSASG-IQLGKLKVFDGGSDLLRIIMGTNFTGLSGRIQFD 387
            + L    NI+FSS  K+      G + LG L +FD GS  L  I+ TN TG++G+IQF 
Sbjct: 330 KRLLDSRANISFSSDPKLTSMKGGGSLNLGALSIFDQGSQFLDYIVNTNMTGVTGQIQFL 389

Query: 388 ADRNIINGSYDVINIDSKGFHTVGYWSNYSGLLVSPPEDLTLKQEDYSRLNQKIESVVWP 447
            DR++I  SYD+IN+   GF  +GYWSN+SGL + PPE L  K  + S  NQ + +V WP
Sbjct: 390 PDRSMIQPSYDIINVVDDGFRQIGYWSNHSGLSIIPPESLYKKLSNRSSSNQHLNNVTWP 449

Query: 448 GGKTETPRGWVIADAGRPLRIAFPKRESFVDFVTQLNNSNVVRGYTIDVFKEALKFIPYD 507
           GG +ETPRGWV  + GR LRI  P R SF +FV++L+ SN V+GY IDVF+ A+K I Y 
Sbjct: 450 GGTSETPRGWVFPNNGRRLRIGVPDRASFKEFVSRLDGSNKVQGYAIDVFEAAVKLISYP 509

Query: 508 VPYEFVPFGDGRANPNYDELVQSVADHVFDAAVGDIAIVTNRTKIVDFSQPYTTTGLIIV 567
           VP+EFV FGDG  NPN++E V +V   VFDA VGDIAIVT RT+IVDF+QPY  +GL++V
Sbjct: 510 VPHEFVLFGDGLKNPNFNEFVNNVTIGVFDAVVGDIAIVTKRTRIVDFTQPYIESGLVVV 569

Query: 568 APVEDSKSSAWVFLRPFTVEMWCVTAASFVIIGIVIWILEHRINDHFRGPPKRQIITMCL 627
           APV     + W FLRPFT  MW VTAA F+I+G VIWILEHRIND FRGPP++QI+T+  
Sbjct: 570 APVTKLNDTPWAFLRPFTPPMWAVTAAFFLIVGSVIWILEHRINDEFRGPPRKQIVTILW 629

Query: 628 FSFSTLFKANQEATISPLSRLVMLVWLFLLLVITSSYTASLTSILTIQQLPSSITGIDDL 687
           FSFST+F +++E T+S L R V+L+WLF++L+ITSSYTASLTSILT+QQL S I G+D L
Sbjct: 630 FSFSTMFFSHRENTVSTLGRAVLLIWLFVVLIITSSYTASLTSILTVQQLNSPIRGVDTL 689

Query: 688 IASNLPIGYQVGSFAYSYLTDSLFIPRSRLVMLYTPEDYERALRVGPKGGGVAAIIDELP 747
           I+S+  +G+QVGS+A +Y+ D L I RSRLV L +P++Y  AL    + G VAAI+DE P
Sbjct: 690 ISSSGRVGFQVGSYAENYMIDELNIARSRLVPLGSPKEYAAAL----QNGTVAAIVDERP 749

Query: 748 YVELFLSNTKEFGMIGQPFTRSGWGFAFQRESRLAVDMSTAILRLSESGKLQELHDSWFC 807
           YV+LFLS    F + GQ FTRSGWGFAF R+S LA+DMSTAIL LSE+G+LQ++HD W  
Sbjct: 750 YVDLFLSEFCGFAIRGQEFTRSGWGFAFPRDSPLAIDMSTAILGLSETGQLQKIHDKWLS 809

Query: 808 KLGCPGERRGKSEPD--QLHLISFWGLYLLCGIISVAALFVFLLLLVRQYIRYKRHRRHF 867
           +  C       S+ D  QL L SFWGL+L+CGI    ALF++   +VR + R+ ++    
Sbjct: 810 RSNCSNLNGSVSDEDSEQLKLRSFWGLFLVCGISCFIALFIYFFKIVRDFFRHGKYDE-- 869

Query: 868 EETTPSPITSHTSCTRSIQSFISFIDEKEEAIK 886
           E T PSP    +S ++S+Q+F+++ DEKE+  K
Sbjct: 870 EATVPSP---ESSRSKSLQTFLAYFDEKEDESK 893