MC04g0156 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0156
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionNADH:ubiquinone reductase (non-electrogenic)
LocationMC04: 1210582 .. 1214971 (+)
RNA-Seq ExpressionMC04g0156
SyntenyMC04g0156
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGTTTGTCTTGACTGCAGTAGCGGAGGTCTATTGGCATATGCAGAATCACGATCAGACACTGGCAATCCTATTGAGGAGTGCCCAAAAAAGAGAGTTCTGGTGCTTGGAACAGGATGGGCCGGTATCAGTTTCCTCAAGGATCTGGATGCTTCAATATATGATGTTCAGGTTGTTTCGCCTCAGAATTATTTCTCATTTACTCCTTTGTTACCTAGTGTTACATGTGGAACAGTTGAAGCACGGAGCATTGTCGAACCAGTTCGAAACATCGTGAAGAAGGTAATTTGTGTGATACAACTCAATTTGATCAATAGCATAGTATGTACTTAATTTATGAGGAGATTTGTTGTTTAATTTGTGTTAAAAATATTGTCCTGTTTGTAATTACTTAATTGGAAATTATGAAGCCGCCTAATTACAACTGTATTACTTCTGCAACAGAGAAAAGGTGAAATTAAATTCTGGGAAGCTGAATGCATTAAGATTGATGCTGCAAACAAAAAGGTTTTCTGCCGGTCTAATGTTGACAATAACTTGGTTGGAAATAGGGAGTTCGATTTAGAATATGACTATTTGGTAATAGCAATGGGAGCACAAGTAAATACTTTTAATACGCCTGGTGTCAAGGAGAACTGTCATTTTCTCAAGGTAATCAGTTTTCTTTCATAGTCCGACATACATCTCTTTGGGCTTAGCCATCTTTCTGAAATTTTGTTAGGCAATTTTTCTATCTCGGTTCTTGCTTCTTTTGTTGATAGCCATATATTCCCATGTTATGATGATTGGGAAACCATTCCATGTCATGCAGGAAGTGGAAGATGCTCTGAAGATCCGCAGAAGTGTTATAGATTGCTTTGAAATGGCTGTTATTCCCAGTCTTAGTGACGAAGAGAGAAGGGAAAATCTTCATTTCGTTATTGTTGGAGGGGGTCCCACTGGGATTGAATTTGCTGCAGAGCTTAATGACTTTTTGGAAGAAGATTTAGTTAATCTATATCCTACGGTTAAAGATCTGGTGAAAATTTCAGTGATTCAATCAGGAGATCACATCTTGAATGCGTAAGGAAAGAATTAAAGTTTACACTAAGCCTGCCTTTTCTGGCACGCGAGAAAATTAATTCAGAACTCACAATTCTGTCAATCTTTTTGGAGCGTACAATGCTTTTGTGTAGCAAGAGAACCTTGACAAAATTAATGCATAAGATACATGCTGTAACCTTCAAGACGATTACTTTTAAAAAAATCTCACTGTCGATTAATATTGATCTATTGGTTAGACAAGTTGATGTAATCTTGCTTGCTTAATGCAGGTTTGATGAAAGAATTAGTTCATTTGCGGAACAAAAGTTCCAAAGAGATGGTATCGATGTGTACACAGGATGTCGGGTTGTCAGTGTATCTGATAAGGACATTAAGATGAAGGTGAAATCAACTGGAGAATTCTGTTCAATACCGCATGGACTAATTGTGTGGTCTACGGGCATCATGACTCGTCCTGTTGTGAAGGATTTCATGGAGCAGATTGGCCAGGTTTGTTGGTTTGAGATTATGCGTTTGTGAGTATGATGGGCATGGAAGAACGGATAAAAACAAAATTATGGATTTCGCTAGACTTGACATGCATTCTTTCTAACTGGGCTTGTATGTTTCACTTGATGCATCGCTTTTCATGGGTTGTTATTCTATGCTTAACTATTTATGTCGCAAGTTCTTTTTGACATACAGGGCAAGAGACGTATTCTAGCTACTGATGAATGGCTTCAAGTGAAGGGCACTCAGAATGTGTATGCCATTGGTGATTGTGCTACAATTGACCAACGCAAAATTATGGTATATGATACTATCACTGCTCAATTATATTTTACTACAAGGTCTGTCTGACAATCTGATTTTGATGAGAGCAATTTTTTACAGCAGTCCTACACTTATCAAATTTGATCGATGTTTTGAAGCTCTTAAATTTTCAACCATTTTATTAATTCACTGAGGCTCTTAAATTCTGTTCATTGTTGAAGTACTTGACTTTTGGGAATCTAACCTCTGGATTTCTCTTTGGCTGCTGAGGAATTTCTCACAAGAGTAGTTTTGTAATTAGTAACTTCAGTTCTTGATTACTTAGTGATTTTAATATAATGTTTTCATTAGGAAGATATCTCCACCATATTTAAAGCTGCAGATAAAGATGATTCTGGAACCTTGACAGTTAAAGAATTTCAAGATGTTATAGATGATATCCTCATAAGGTATCCTCAAGTGGAAATATATTTGAGGAGCAAACATCTACGCGATGTTAAAGATCTGTTGAAAGATTCTCAGGGGCATGAGAACGGTGTAGATATTGAAGGATTTAAGTCAGCTCTTTCTCACGCTGACTCGCAGATGAAAAGTCTTCCTGCTACTGCACAGGTAAATGCACATATTTGTTAATGTCGACTTGTGACATGTTCAACTCAGCTCTCATGTGAGCTTGCAATCTACATAGGAAAGACTCTCAAGTCATTTAATGAATCCTTCCCCGTTCAGGTTGCTGCCCAGCAGGGTGCGTATCTTTCCAGGTGCTTCAACCGCAGGGAGCATTGCACTGAGAATCCTGAAGGCCCACGACAGTTTAAGAGTTCTGGACGTCATCTATTTCTTCCCTTTCGGTATGCCTGCAGCTTCAATCCTTATCCTTTCTATCCCATGTTCCATGCCGGATGAAGTAGTTTAAGTTAACATCTTAATGAAGTTCAATTGTTATGCTTTAAGTGTGTGTGTGTGTGAAACCTGCTATAAATGAAAGTACGAAAGACAAGGACATCAAAAAGAGCCCATTGAACTGAAACCAACAAAGAAAGAAGCAGAAATAAAAATAATTTCATTAGGACTCTTCAATTGTTTAGGATCTTGGATGAGTGACAACTACCAAAAAAGAAAAAAGAAAAAAAGTCGAAGGAAGCACCCAAAAAGAGGCATGGAAGTGAGCTGCGCTCTAAACCTCCGCCCAAGAAATCTAACCCTCATCGAGATTATTTTATCTCTCTATCCAGATATATATCAAAAAATGTTGCTACAGAAGTGTCCTCCATTACAAAAGATGTGGTGAAGAAACACCTCCTCCATACTATCCCAACAATCCCTATTAGAATTCAAGCAAACGCCAAATGTTAAGGAAATCTATCCCAAAATATCAAAACAAAATGACAAAGTATGGAGAAAGGACAATTAACTAAGTTTCAAATCTCAAGGGGCAAGCACTGAGGTATAAATGCCATAAGTGCTAGTTTTTAGCTAGACATAGTAGTTGATGCCAAATTCATGTCTGAGGAACTCAATTAATCTGTTTAAGAAACACCTTCAGAATTTCTAGCAAATAGATCATCCTTTATGAAACTGGTATTTAGTACCTTCTTTGACATGTTATAAAGAGATTTTATATTGCTATCTCATGGGGCACGATTAGATAACAAAAAAGTTCATTAATAGTTGCACCGTAACCCTTCTCTCTGCATCTGGGCCAAAGAGTTTGCATTCACCATGACTTACAGGTACAAGCACTTGGGACAGTTCGCTCCTTTAGGAGGAGAACAAGCTGCTGCAGAACTCCCTGGAGACTTGGTTTCCATGGGTCGCAGCACTCAGTGGCTCTGGTATTCTGTATATGCAAGGTGATTTAACTGCTGCTTTTCATCAGAAATTTTCAACTTATTTACACAGTTTAGTTGTAACTCAATGCTTGATCTGAATTCTTCTGTTTTCGTACCCAGCAAGCAGGTCAGCTGGCGCACAAGGTTTCTAGTAGTATCAGACTGGACAAGAAAGTTTATCTTCGGGAGAGACTCAAGCCGCATCTGAAACTAACCACCATTTAAAGGGCTTCAACATATCTTCAAGTTCAAGGCTTTTGAACAATCACTTCTGCGTGTTCGCTCGATCTTCGGTGGCCCTCACTATTTTAACTCTACGCCTGATTTCCATGCTTGGTTTCAACGTAATTGTTCAGTTTTTTCTTTTTGAACAAAAATAACTTTTTATTGATAAAAAGTTGTAATTGTTCATTAGCAGATGGCAAAGGAAATAACAGTTCGTATTGGTACAAAATGTTATTTAGCTTCACGTTTCAATTGCTGAGTGGCTCAATGAAAGATAATTTTTTAAGATATTTTCACCAGTGAAGCGAGTAACAGCAACGCCCTTTCAGAAGATGCCGTGTAGCCATGGATGTTAGATTTCAAGCTAACCCTTCACAATAAGTTGCAGGTTCTTCTCAGGCTCTTAACTCTGAACCATTATTATTCTCTATCTTAGATATAATTAAATTTATCCAACTCATCAAGTTAAACTTTTGGATTCTATAGTAGTTTATTTATTCTTAAGATGATATTAGAACGGGAGATTCTATGTTCAA

mRNA sequence

GTGGTTTGTCTTGACTGCAGTAGCGGAGGTCTATTGGCATATGCAGAATCACGATCAGACACTGGCAATCCTATTGAGGAGTGCCCAAAAAAGAGAGTTCTGGTGCTTGGAACAGGATGGGCCGGTATCAGTTTCCTCAAGGATCTGGATGCTTCAATATATGATGTTCAGGTTGTTTCGCCTCAGAATTATTTCTCATTTACTCCTTTGTTACCTAGTGTTACATGTGGAACAGTTGAAGCACGGAGCATTGTCGAACCAGTTCGAAACATCGTGAAGAAGAGAAAAGGTGAAATTAAATTCTGGGAAGCTGAATGCATTAAGATTGATGCTGCAAACAAAAAGGTTTTCTGCCGGTCTAATGTTGACAATAACTTGGTTGGAAATAGGGAGTTCGATTTAGAATATGACTATTTGGTAATAGCAATGGGAGCACAAGTAAATACTTTTAATACGCCTGGTGTCAAGGAGAACTGTCATTTTCTCAAGGAAGTGGAAGATGCTCTGAAGATCCGCAGAAGTGTTATAGATTGCTTTGAAATGGCTGTTATTCCCAGTCTTAGTGACGAAGAGAGAAGGGAAAATCTTCATTTCGTTATTGTTGGAGGGGGTCCCACTGGGATTGAATTTGCTGCAGAGCTTAATGACTTTTTGGAAGAAGATTTAGTTAATCTATATCCTACGGTTAAAGATCTGGTGAAAATTTCAGTGATTCAATCAGGAGATCACATCTTGAATGCGTTTGATGAAAGAATTAGTTCATTTGCGGAACAAAAGTTCCAAAGAGATGGTATCGATGTGTACACAGGATGTCGGGTTGTCAGTGTATCTGATAAGGACATTAAGATGAAGGTGAAATCAACTGGAGAATTCTGTTCAATACCGCATGGACTAATTGTGTGGTCTACGGGCATCATGACTCGTCCTGTTGTGAAGGATTTCATGGAGCAGATTGGCCAGGGCAAGAGACGTATTCTAGCTACTGATGAATGGCTTCAAGTGAAGGGCACTCAGAATGTGTATGCCATTGGTGATTGTGCTACAATTGACCAACGCAAAATTATGGAAGATATCTCCACCATATTTAAAGCTGCAGATAAAGATGATTCTGGAACCTTGACAGTTAAAGAATTTCAAGATGTTATAGATGATATCCTCATAAGGTATCCTCAAGTGGAAATATATTTGAGGAGCAAACATCTACGCGATGTTAAAGATCTGTTGAAAGATTCTCAGGGGCATGAGAACGGTGTAGATATTGAAGGATTTAAGTCAGCTCTTTCTCACGCTGACTCGCAGATGAAAAGTCTTCCTGCTACTGCACAGGTTGCTGCCCAGCAGGGTGCGTATCTTTCCAGGTGCTTCAACCGCAGGGAGCATTGCACTGAGAATCCTGAAGGCCCACGACAGTTTAAGAGTTCTGGACGTCATCTATTTCTTCCCTTTCGGTACAAGCACTTGGGACAGTTCGCTCCTTTAGGAGGAGAACAAGCTGCTGCAGAACTCCCTGGAGACTTGGTTTCCATGGGTCGCAGCACTCAGTGGCTCTGGTATTCTGTATATGCAAGCAAGCAGGTCAGCTGGCGCACAAGGTTTCTAGTAGTATCAGACTGGACAAGAAAGTTTATCTTCGGGAGAGACTCAAGCCGCATCTGAAACTAACCACCATTTAAAGGGCTTCAACATATCTTCAAGTTCAAGGCTTTTGAACAATCACTTCTGCGTGTTCGCTCGATCTTCGGTGGCCCTCACTATTTTAACTCTACGCCTGATTTCCATGCTTGGTTTCAACGTAATTGTTCAGTTTTTTCTTTTTGAACAAAAATAACTTTTTATTGATAAAAAGTTGTAATTGTTCATTAGCAGATGGCAAAGGAAATAACAGTTCGTATTGGTACAAAATGTTATTTAGCTTCACGTTTCAATTGCTGAGTGGCTCAATGAAAGATAATTTTTTAAGATATTTTCACCAGTGAAGCGAGTAACAGCAACGCCCTTTCAGAAGATGCCGTGTAGCCATGGATGTTAGATTTCAAGCTAACCCTTCACAATAAGTTGCAGGTTCTTCTCAGGCTCTTAACTCTGAACCATTATTATTCTCTATCTTAGATATAATTAAATTTATCCAACTCATCAAGTTAAACTTTTGGATTCTATAGTAGTTTATTTATTCTTAAGATGATATTAGAACGGGAGATTCTATGTTCAA

Coding sequence (CDS)

GTGGTTTGTCTTGACTGCAGTAGCGGAGGTCTATTGGCATATGCAGAATCACGATCAGACACTGGCAATCCTATTGAGGAGTGCCCAAAAAAGAGAGTTCTGGTGCTTGGAACAGGATGGGCCGGTATCAGTTTCCTCAAGGATCTGGATGCTTCAATATATGATGTTCAGGTTGTTTCGCCTCAGAATTATTTCTCATTTACTCCTTTGTTACCTAGTGTTACATGTGGAACAGTTGAAGCACGGAGCATTGTCGAACCAGTTCGAAACATCGTGAAGAAGAGAAAAGGTGAAATTAAATTCTGGGAAGCTGAATGCATTAAGATTGATGCTGCAAACAAAAAGGTTTTCTGCCGGTCTAATGTTGACAATAACTTGGTTGGAAATAGGGAGTTCGATTTAGAATATGACTATTTGGTAATAGCAATGGGAGCACAAGTAAATACTTTTAATACGCCTGGTGTCAAGGAGAACTGTCATTTTCTCAAGGAAGTGGAAGATGCTCTGAAGATCCGCAGAAGTGTTATAGATTGCTTTGAAATGGCTGTTATTCCCAGTCTTAGTGACGAAGAGAGAAGGGAAAATCTTCATTTCGTTATTGTTGGAGGGGGTCCCACTGGGATTGAATTTGCTGCAGAGCTTAATGACTTTTTGGAAGAAGATTTAGTTAATCTATATCCTACGGTTAAAGATCTGGTGAAAATTTCAGTGATTCAATCAGGAGATCACATCTTGAATGCGTTTGATGAAAGAATTAGTTCATTTGCGGAACAAAAGTTCCAAAGAGATGGTATCGATGTGTACACAGGATGTCGGGTTGTCAGTGTATCTGATAAGGACATTAAGATGAAGGTGAAATCAACTGGAGAATTCTGTTCAATACCGCATGGACTAATTGTGTGGTCTACGGGCATCATGACTCGTCCTGTTGTGAAGGATTTCATGGAGCAGATTGGCCAGGGCAAGAGACGTATTCTAGCTACTGATGAATGGCTTCAAGTGAAGGGCACTCAGAATGTGTATGCCATTGGTGATTGTGCTACAATTGACCAACGCAAAATTATGGAAGATATCTCCACCATATTTAAAGCTGCAGATAAAGATGATTCTGGAACCTTGACAGTTAAAGAATTTCAAGATGTTATAGATGATATCCTCATAAGGTATCCTCAAGTGGAAATATATTTGAGGAGCAAACATCTACGCGATGTTAAAGATCTGTTGAAAGATTCTCAGGGGCATGAGAACGGTGTAGATATTGAAGGATTTAAGTCAGCTCTTTCTCACGCTGACTCGCAGATGAAAAGTCTTCCTGCTACTGCACAGGTTGCTGCCCAGCAGGGTGCGTATCTTTCCAGGTGCTTCAACCGCAGGGAGCATTGCACTGAGAATCCTGAAGGCCCACGACAGTTTAAGAGTTCTGGACGTCATCTATTTCTTCCCTTTCGGTACAAGCACTTGGGACAGTTCGCTCCTTTAGGAGGAGAACAAGCTGCTGCAGAACTCCCTGGAGACTTGGTTTCCATGGGTCGCAGCACTCAGTGGCTCTGGTATTCTGTATATGCAAGCAAGCAGGTCAGCTGGCGCACAAGGTTTCTAGTAGTATCAGACTGGACAAGAAAGTTTATCTTCGGGAGAGACTCAAGCCGCATCTGA

Protein sequence

VVCLDCSSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNLVGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPSLSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILNAFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIMTRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAADKDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKHLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGRDSSRI
Homology
BLAST of MC04g0156 vs. ExPASy Swiss-Prot
Match: M1BYJ7 (External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial OS=Solanum tuberosum OX=4113 GN=NDB1 PE=3 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 1.4e-253
Identity = 417/557 (74.87%), Postives = 482/557 (86.54%), Query Frame = 0

Query: 1   VVCLDCSSGGLLAYAESRSDTGNPI-----EECPKKRVLVLGTGWAGISFLKDLDASIYD 60
           +V    S+GGLL YAES  ++G  +      E  KKRV+VLGTGW G SFLKD+D S YD
Sbjct: 21  LVLCSVSTGGLLVYAESNVESGKQVVEQNQPESKKKRVVVLGTGWGGTSFLKDVDISSYD 80

Query: 61  VQVVSPQNYFSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKK 120
           VQVVSP+NYF+FTPLLPSVTCGTVEARSIVEPVRNI+KKR GEI+FWEAEC+KID  N+ 
Sbjct: 81  VQVVSPRNYFAFTPLLPSVTCGTVEARSIVEPVRNIIKKRSGEIQFWEAECLKIDPENRT 140

Query: 121 VFCRSNVDNNLVGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSV 180
           V CRS +++NL G  +F L+YDYLV+A+GAQVNTFNTPGV E+CHFLKEVEDA +IRR+V
Sbjct: 141 VSCRSGINDNLAGQNDFSLQYDYLVVAVGAQVNTFNTPGVMEHCHFLKEVEDAQRIRRTV 200

Query: 181 IDCFEMAVIPSLSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKI 240
           IDCFE +VIP LS+EERR NLHFVIVGGGPTG+EFAAEL+D++ EDLV +YP+VKD VKI
Sbjct: 201 IDCFEKSVIPGLSEEERRTNLHFVIVGGGPTGVEFAAELHDYVYEDLVKIYPSVKDFVKI 260

Query: 241 SVIQSGDHILNAFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIP 300
           +VIQSGDHILN FDERISSFAEQKFQRDGI+V TGCRV SVSD  I MKVKSTG+   +P
Sbjct: 261 TVIQSGDHILNTFDERISSFAEQKFQRDGIEVSTGCRVTSVSDHFINMKVKSTGKHVEVP 320

Query: 301 HGLIVWSTGIMTRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIM 360
           +G++VWSTG+ TRP VKDFMEQ+GQ KRRILATDEWL+VKG  NVYA+GDCA+IDQRK+M
Sbjct: 321 YGMVVWSTGVGTRPFVKDFMEQVGQEKRRILATDEWLRVKGCSNVYALGDCASIDQRKVM 380

Query: 361 EDISTIFKAADKDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHE 420
           EDIS IFKAADKDDSGTL+++EF+DV++DI+IRYPQV++YL++KHL + KDL +DS+G+E
Sbjct: 381 EDISAIFKAADKDDSGTLSIEEFRDVLEDIIIRYPQVDLYLKNKHLLEAKDLFRDSEGNE 440

Query: 421 -NGVDIEGFKSALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSS 480
              VDIEGFK ALSH DSQMKSLPATAQVAAQQG YLSRC NR + C  NPEGPR FKSS
Sbjct: 441 REEVDIEGFKLALSHVDSQMKSLPATAQVAAQQGTYLSRCLNRWDQCKSNPEGPRHFKSS 500

Query: 481 GRHLFLPFRYKHLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLV 540
           GRH FLPF Y+HLGQFAPLGG+QAAAELPGD VSMG STQWLWYSVYASKQVSWRTR+LV
Sbjct: 501 GRHEFLPFEYRHLGQFAPLGGDQAAAELPGDWVSMGHSTQWLWYSVYASKQVSWRTRYLV 560

Query: 541 VSDWTRKFIFGRDSSRI 552
           V DW R++IFGRDSSRI
Sbjct: 561 VGDWVRRYIFGRDSSRI 577

BLAST of MC04g0156 vs. ExPASy Swiss-Prot
Match: Q9ST62 (External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial OS=Solanum tuberosum OX=4113 GN=NDB1 PE=1 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 4.0e-253
Identity = 415/557 (74.51%), Postives = 484/557 (86.89%), Query Frame = 0

Query: 1   VVCLDCSSGGLLAYAESRSDTGNPI-----EECPKKRVLVLGTGWAGISFLKDLDASIYD 60
           +V    S+GGLL YAES  ++G  +      E  KKRV+VLGTGW G SFLKD+D S YD
Sbjct: 21  LVLCSVSTGGLLVYAESNVESGKQVVEQNQPESKKKRVVVLGTGWGGTSFLKDVDISSYD 80

Query: 61  VQVVSPQNYFSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKK 120
           VQVVSP+NYF+FTPLLPSVTCGTVEARSIVEPVRNI+KKR GEI+FWEAEC+KID  N+ 
Sbjct: 81  VQVVSPRNYFAFTPLLPSVTCGTVEARSIVEPVRNIIKKRSGEIQFWEAECLKIDPVNRT 140

Query: 121 VFCRSNVDNNLVGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSV 180
           V CRS +++NL G+ +F L+YDYLV+A+GAQVNTFNTPGV E+CHFLKEVEDA +IRR+V
Sbjct: 141 VSCRSGINDNLAGHNDFSLQYDYLVVAVGAQVNTFNTPGVMEHCHFLKEVEDAQRIRRTV 200

Query: 181 IDCFEMAVIPSLSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKI 240
           IDCFE +VIP LS+EERR NLHFVIVGGGPTG+EFAAEL+D++ EDLV +YP+VKD VKI
Sbjct: 201 IDCFEKSVIPGLSEEERRTNLHFVIVGGGPTGVEFAAELHDYVYEDLVKIYPSVKDFVKI 260

Query: 241 SVIQSGDHILNAFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIP 300
           +VIQSGDHILN FDERISSFAEQKFQRDGI+V TGCRV SVSD  I MKVKSTG+   +P
Sbjct: 261 TVIQSGDHILNTFDERISSFAEQKFQRDGIEVSTGCRVTSVSDHFINMKVKSTGKHVEVP 320

Query: 301 HGLIVWSTGIMTRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIM 360
           +G++VWSTG+ TRP VKDFMEQ+GQ KRRILATDEWL+VKG  NVYA+GDCA++DQ K+M
Sbjct: 321 YGMVVWSTGVGTRPFVKDFMEQVGQEKRRILATDEWLRVKGCSNVYALGDCASVDQHKVM 380

Query: 361 EDISTIFKAADKDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHE 420
           EDISTIF+AADKDDSGTL+V+EF+DV++DI+IRYPQV++YL++KHL + KDL +DS+G+E
Sbjct: 381 EDISTIFEAADKDDSGTLSVEEFRDVLEDIIIRYPQVDLYLKNKHLLEAKDLFRDSEGNE 440

Query: 421 -NGVDIEGFKSALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSS 480
              VDIEGFK ALSH DSQMKSLPATAQVAAQQG YL+RC NR + C  NPEGPR+FKSS
Sbjct: 441 REEVDIEGFKLALSHVDSQMKSLPATAQVAAQQGTYLARCLNRWDQCKSNPEGPRRFKSS 500

Query: 481 GRHLFLPFRYKHLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLV 540
           GRH FLPF Y+HLGQFAPLGG+QAAAELPGD VSMG STQWLWYSVYASKQVSWRTR+LV
Sbjct: 501 GRHEFLPFEYRHLGQFAPLGGDQAAAELPGDWVSMGHSTQWLWYSVYASKQVSWRTRYLV 560

Query: 541 VSDWTRKFIFGRDSSRI 552
           V DW R++IFGRDSSRI
Sbjct: 561 VGDWVRRYIFGRDSSRI 577

BLAST of MC04g0156 vs. ExPASy Swiss-Prot
Match: Q1JPL4 (External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NDB1 PE=1 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 3.4e-236
Identity = 389/546 (71.25%), Postives = 465/546 (85.16%), Query Frame = 0

Query: 7   SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 66
           S G ++AYA++ ++  N  EE  KK+V+VLGTGWAGISFLKDLD + YDVQVVSPQNYF+
Sbjct: 27  SGGSIVAYADA-NEEANKKEEHKKKKVVVLGTGWAGISFLKDLDITSYDVQVVSPQNYFA 86

Query: 67  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 126
           FTPLLPSVTCGTVEARSIVE VRNI KK+ GEI+ WEA+C KID  N+KV CR    ++ 
Sbjct: 87  FTPLLPSVTCGTVEARSIVESVRNITKKKNGEIELWEADCFKIDHVNQKVHCRPVFKDDP 146

Query: 127 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 186
             ++EF L YDYL++A+GAQVNTF TPGV ENCHFLKEVEDA +IRR VIDCFE A++P 
Sbjct: 147 EASQEFSLGYDYLIVAVGAQVNTFGTPGVLENCHFLKEVEDAQRIRRGVIDCFEKAILPG 206

Query: 187 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 246
           L++E+RR  LHFVIVGGGPTG+EFAAEL+DF+ ED+  +YP+VK+LVKI++IQSGDHILN
Sbjct: 207 LTEEQRRRKLHFVIVGGGPTGVEFAAELHDFIIEDITKIYPSVKELVKITLIQSGDHILN 266

Query: 247 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 306
            FDERISSFAEQKF RDGIDV TG RV+SV+DKDI +KVKS+GE  SIPHGLI+WSTG+ 
Sbjct: 267 TFDERISSFAEQKFTRDGIDVQTGMRVMSVTDKDITVKVKSSGELVSIPHGLILWSTGVG 326

Query: 307 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 366
           TRPV+ DFMEQ+GQG RR +AT+EWLQV G +NVYA+GDCA+I QRKI+ DI+ IFKAAD
Sbjct: 327 TRPVISDFMEQVGQGGRRAVATNEWLQVTGCENVYAVGDCASIAQRKILGDIANIFKAAD 386

Query: 367 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGH-ENGVDIEGFKS 426
            D+SGTLT++E + V+DDI++RYPQVE+YL+SKH+R + DLL DS+G+    VDIE FK 
Sbjct: 387 ADNSGTLTMEELEGVVDDIIVRYPQVELYLKSKHMRHINDLLADSEGNARKEVDIEAFKL 446

Query: 427 ALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYK 486
           ALS ADSQMK+LPATAQVAAQQGAYL++CFNR E C E PEGP++F++ G H F PF+YK
Sbjct: 447 ALSEADSQMKTLPATAQVAAQQGAYLAKCFNRMEQCKELPEGPKRFRTGGHHQFRPFQYK 506

Query: 487 HLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFG 546
           H GQFAPLGG+QAAAELPGD VS G+S QWLWYSVYASKQVSWRTR LVVSDWTR++IFG
Sbjct: 507 HFGQFAPLGGDQAAAELPGDWVSAGKSAQWLWYSVYASKQVSWRTRALVVSDWTRRYIFG 566

Query: 547 RDSSRI 552
           RDSSRI
Sbjct: 567 RDSSRI 571

BLAST of MC04g0156 vs. ExPASy Swiss-Prot
Match: Q94BV7 (External alternative NAD(P)H-ubiquinone oxidoreductase B2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NDB2 PE=1 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 7.4e-215
Identity = 362/555 (65.23%), Postives = 448/555 (80.72%), Query Frame = 0

Query: 7   SSGGLLAYAESRSD---TGNPIEE--CPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSP 66
           S GGL+AY+E+ +     G  + E    KK+V++LGTGWAG SFLK+L+ S Y+VQ++SP
Sbjct: 30  SGGGLIAYSEANASYGANGGAVVETGTKKKKVVLLGTGWAGTSFLKNLNNSQYEVQIISP 89

Query: 67  QNYFSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSN 126
           +NYF+FTPLLPSVTCGTVEARS+VEP+RNI +K   +  + EAEC KID A+KKV+CRS 
Sbjct: 90  RNYFAFTPLLPSVTCGTVEARSVVEPIRNIGRKNV-DTSYLEAECFKIDPASKKVYCRSK 149

Query: 127 VDNNLVGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEM 186
              +  G +EF ++YDYLVIA GAQ NTFN PGV+ENCHFLKEVEDA +IR++VID FE 
Sbjct: 150 QGLSSNGKKEFSVDYDYLVIATGAQSNTFNIPGVEENCHFLKEVEDAQRIRKTVIDSFEK 209

Query: 187 AVIPSLSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSG 246
           A +P LSDEER+  LHFV+VGGGPTG+EFAAEL+DF+ EDLV+LYP  K  V+I+++++ 
Sbjct: 210 ASLPELSDEERKRILHFVVVGGGPTGVEFAAELHDFVTEDLVSLYPRAKGSVRITLLEAA 269

Query: 247 DHILNAFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVW 306
           DHIL  FD+RI+ FAE+KF RDGIDV  G  V  V++KDI  K K  GE  SIP+G+IVW
Sbjct: 270 DHILTMFDKRITEFAEEKFSRDGIDVKLGSMVTKVNEKDISAKTKG-GEVSSIPYGMIVW 329

Query: 307 STGIMTRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTI 366
           STGI TRPV+KDFM+QIGQG RR LATDEWL+V+GT N+YA+GDCATI+QRK+MED+S I
Sbjct: 330 STGIGTRPVIKDFMKQIGQGNRRALATDEWLRVEGTDNIYALGDCATINQRKVMEDVSAI 389

Query: 367 FKAADKDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHE---NGV 426
           F  ADKD SGTLT+KEFQ+ +DDI +RYPQVE+YL+SK +R + DLLK+++  +   N +
Sbjct: 390 FSKADKDKSGTLTLKEFQEAMDDICVRYPQVELYLKSKRMRGIADLLKEAETDDVSKNNI 449

Query: 427 D--IEGFKSALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGR 486
           +  IE FKSALS  DSQ+K LPATAQVAAQQGAYL++CF+R E C ++PEGP + +  GR
Sbjct: 450 ELKIEEFKSALSQVDSQVKFLPATAQVAAQQGAYLAKCFDRMEECEKSPEGPIRMRGEGR 509

Query: 487 HLFLPFRYKHLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVS 546
           H F PFRY+HLGQFAPLGGEQ AA+LPGD VS+G S+QWLWYSVYASKQVSWRTR LVVS
Sbjct: 510 HRFRPFRYRHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRVLVVS 569

Query: 547 DWTRKFIFGRDSSRI 552
           DW R+FIFGRDSS I
Sbjct: 570 DWMRRFIFGRDSSSI 582

BLAST of MC04g0156 vs. ExPASy Swiss-Prot
Match: F4JJJ3 (External alternative NAD(P)H-ubiquinone oxidoreductase B3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NDB3 PE=2 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 9.6e-215
Identity = 361/553 (65.28%), Postives = 447/553 (80.83%), Query Frame = 0

Query: 7   SSGGLLAYAESR-SDTGNPIE-ECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNY 66
           S GGL+ Y+E+  S + N +E +  K++V++LGTGWAG SFLK L+ S Y+VQV+SP+NY
Sbjct: 30  SGGGLIVYSEANPSYSNNGVETKTRKRKVVLLGTGWAGASFLKTLNNSSYEVQVISPRNY 89

Query: 67  FSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDN 126
           F+FTPLLPSVTCGTVEARS+VEP+RNI +K+  E+ F EAEC KID  +KKV+CRS    
Sbjct: 90  FAFTPLLPSVTCGTVEARSVVEPIRNIARKQNVEMSFLEAECFKIDPGSKKVYCRSKQGV 149

Query: 127 NLVGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVI 186
           N  G +EFD++YDYLVIA GAQ NTFN PGV+ENCHFLKEVEDA +IR +VID FE A +
Sbjct: 150 NSKGKKEFDVDYDYLVIATGAQSNTFNIPGVEENCHFLKEVEDAQRIRSTVIDSFEKASL 209

Query: 187 PSLSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHI 246
           P L+++ER+  LHFV+VGGGPTG+EFA+EL+DF+ EDLV LYP  K+LV+I+++++ DHI
Sbjct: 210 PGLNEQERKRMLHFVVVGGGPTGVEFASELHDFVNEDLVKLYPKAKNLVQITLLEAADHI 269

Query: 247 LNAFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTG 306
           L  FD+RI+ FAE+KF RDGIDV  G  VV V+DK+I  K K+ GE  +IP+G+IVWSTG
Sbjct: 270 LTMFDKRITEFAEEKFTRDGIDVKLGSMVVKVNDKEISAKTKA-GEVSTIPYGMIVWSTG 329

Query: 307 IMTRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKA 366
           I TRPV+KDFM+QIGQG RR LATDEWL+V+G  N+YA+GDCATI+QRK+MEDI+ IFK 
Sbjct: 330 IGTRPVIKDFMKQIGQGNRRALATDEWLRVEGCDNIYALGDCATINQRKVMEDIAAIFKK 389

Query: 367 ADKDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENG------V 426
           ADK++SGTLT+KEF +V+ DI  RYPQVE+YL+SK +  + DLLK +Q  ENG      +
Sbjct: 390 ADKENSGTLTMKEFHEVMSDICDRYPQVELYLKSKGMHGITDLLKQAQA-ENGSNKSVEL 449

Query: 427 DIEGFKSALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHL 486
           DIE  KSAL   DSQ+K LPAT QVAAQQG YL++CF+R E C +NPEGP + +  GRH 
Sbjct: 450 DIEELKSALCQVDSQVKLLPATGQVAAQQGTYLAKCFDRMEVCEKNPEGPIRIRGEGRHR 509

Query: 487 FLPFRYKHLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDW 546
           F PFRY+HLGQFAPLGGEQ AA+LPGD VS+G S+QWLWYSVYASKQVSWRTR LVVSDW
Sbjct: 510 FRPFRYRHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRVLVVSDW 569

Query: 547 TRKFIFGRDSSRI 552
            R+FIFGRDSSRI
Sbjct: 570 MRRFIFGRDSSRI 580

BLAST of MC04g0156 vs. NCBI nr
Match: XP_022148080.1 (external alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 7   SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 66
           SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS
Sbjct: 30  SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 89

Query: 67  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 126
           FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL
Sbjct: 90  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 149

Query: 127 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 186
           VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS
Sbjct: 150 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 209

Query: 187 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 246
           LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN
Sbjct: 210 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 269

Query: 247 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 306
           AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM
Sbjct: 270 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 329

Query: 307 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 366
           TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD
Sbjct: 330 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 389

Query: 367 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA 426
           KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA
Sbjct: 390 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA 449

Query: 427 LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH 486
           LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH
Sbjct: 450 LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH 509

Query: 487 LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR 546
           LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR
Sbjct: 510 LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR 569

Query: 547 DSSRI 551
           DSSRI
Sbjct: 570 DSSRI 574

BLAST of MC04g0156 vs. NCBI nr
Match: XP_022148081.1 (external alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 1097 bits (2836), Expect = 0.0
Identity = 544/544 (100.00%), Postives = 544/544 (100.00%), Query Frame = 0

Query: 8   SGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFSF 67
           SGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFSF
Sbjct: 9   SGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFSF 68

Query: 68  TPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNLV 127
           TPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNLV
Sbjct: 69  TPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNLV 128

Query: 128 GNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPSL 187
           GNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPSL
Sbjct: 129 GNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPSL 188

Query: 188 SDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILNA 247
           SDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILNA
Sbjct: 189 SDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILNA 248

Query: 248 FDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIMT 307
           FDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIMT
Sbjct: 249 FDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIMT 308

Query: 308 RPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAADK 367
           RPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAADK
Sbjct: 309 RPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAADK 368

Query: 368 DDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSAL 427
           DDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSAL
Sbjct: 369 DDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSAL 428

Query: 428 SHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKHL 487
           SHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKHL
Sbjct: 429 SHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKHL 488

Query: 488 GQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGRD 547
           GQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGRD
Sbjct: 489 GQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGRD 548

Query: 548 SSRI 551
           SSRI
Sbjct: 549 SSRI 552

BLAST of MC04g0156 vs. NCBI nr
Match: XP_038888314.1 (external alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 1022 bits (2642), Expect = 0.0
Identity = 503/545 (92.29%), Postives = 525/545 (96.33%), Query Frame = 0

Query: 7   SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 66
           SSGG+LAYA+S+SD  +PIEECPKKRVLVLGTGWAG SFLKDLDAS YDVQVVSPQNYFS
Sbjct: 30  SSGGVLAYADSQSDVASPIEECPKKRVLVLGTGWAGTSFLKDLDASKYDVQVVSPQNYFS 89

Query: 67  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 126
           FTPLLPSVTCG+VEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL
Sbjct: 90  FTPLLPSVTCGSVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 149

Query: 127 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 186
           VGNREF LEYDYLVIAMGAQVNTFNTPGVKENC+FLKEVEDA KIRRSVIDCFEMAVIPS
Sbjct: 150 VGNREFALEYDYLVIAMGAQVNTFNTPGVKENCYFLKEVEDAQKIRRSVIDCFEMAVIPS 209

Query: 187 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 246
           LS+EERR NLHFVIVGGGPTG+EFAAEL+DF  EDLVNLYP+VKDLVKISVIQSGDHILN
Sbjct: 210 LSEEERRRNLHFVIVGGGPTGVEFAAELHDFFVEDLVNLYPSVKDLVKISVIQSGDHILN 269

Query: 247 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 306
           AFDERISSFAEQKF RDGIDVY GCRVVSVSDK+IKMKVKSTGE CS+PHGLIVWSTGIM
Sbjct: 270 AFDERISSFAEQKFLRDGIDVYIGCRVVSVSDKEIKMKVKSTGELCSMPHGLIVWSTGIM 329

Query: 307 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 366
           TRPVVKDFMEQIGQG RRILATDEWLQVKG QNVYAIGDCATIDQRKIMEDI+ IFKAAD
Sbjct: 330 TRPVVKDFMEQIGQGSRRILATDEWLQVKGAQNVYAIGDCATIDQRKIMEDIAAIFKAAD 389

Query: 367 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA 426
           +D+SGTLTV EFQDV+DDILIRYPQVEI+LRSKHLRDVKDLLKDSQGHEN +DIEGFKSA
Sbjct: 390 RDNSGTLTVAEFQDVLDDILIRYPQVEIFLRSKHLRDVKDLLKDSQGHENEIDIEGFKSA 449

Query: 427 LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH 486
           LSHAD+QMKSLPATAQVAAQQGAYLSRCFNRRE+CTENPEGPR+FKSSGRH FLPFRYKH
Sbjct: 450 LSHADTQMKSLPATAQVAAQQGAYLSRCFNRREYCTENPEGPRRFKSSGRHQFLPFRYKH 509

Query: 487 LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR 546
           LGQFAPLGGEQAAAELPGD VSMG STQWLWYSVYASKQVSWRTR+LVVSDWTRKFIFGR
Sbjct: 510 LGQFAPLGGEQAAAELPGDWVSMGHSTQWLWYSVYASKQVSWRTRYLVVSDWTRKFIFGR 569

Query: 547 DSSRI 551
           DSSRI
Sbjct: 570 DSSRI 574

BLAST of MC04g0156 vs. NCBI nr
Match: XP_008455351.1 (PREDICTED: external alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial [Cucumis melo])

HSP 1 Score: 1016 bits (2628), Expect = 0.0
Identity = 500/545 (91.74%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 7   SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 66
           S GGLLAYA+S+SD G  IEE PKKRV+VLGTGWAG SFLKDLDAS YDVQVVSPQNYFS
Sbjct: 31  SGGGLLAYADSQSDVGGSIEESPKKRVVVLGTGWAGTSFLKDLDASKYDVQVVSPQNYFS 90

Query: 67  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 126
           FTPLLPSVTCG+VEARSIVEPVRNIVKKRKGEIKFWEAEC KIDAANKKVFC+S+VDNNL
Sbjct: 91  FTPLLPSVTCGSVEARSIVEPVRNIVKKRKGEIKFWEAECFKIDAANKKVFCQSHVDNNL 150

Query: 127 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 186
           VGNREF LEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDA KIRRSVIDCFEMAVIPS
Sbjct: 151 VGNREFSLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDAQKIRRSVIDCFEMAVIPS 210

Query: 187 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 246
           LS+EERR NLHFVIVGGGPTG+EFAAEL+DF EEDLVNLYP+VKDLVKISVIQSGDHILN
Sbjct: 211 LSEEERRRNLHFVIVGGGPTGVEFAAELHDFFEEDLVNLYPSVKDLVKISVIQSGDHILN 270

Query: 247 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 306
           AFDERIS FAEQKF RDGIDVYTGCRVVSVSDK+I+MKVKSTGE CS+PHGLIVWSTGIM
Sbjct: 271 AFDERISLFAEQKFLRDGIDVYTGCRVVSVSDKEIEMKVKSTGESCSMPHGLIVWSTGIM 330

Query: 307 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 366
           TRPVVKDFMEQIGQG RRILATDEWLQVKG QNVYAIGDCATIDQRKIMEDI+TIFKAAD
Sbjct: 331 TRPVVKDFMEQIGQGSRRILATDEWLQVKGAQNVYAIGDCATIDQRKIMEDIATIFKAAD 390

Query: 367 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA 426
           KD+SGTLTV EFQDV+DDILIRYPQVEI+LRSKHLRDVKDLL+DSQGHEN +DIEGFKSA
Sbjct: 391 KDNSGTLTVAEFQDVLDDILIRYPQVEIFLRSKHLRDVKDLLRDSQGHENEIDIEGFKSA 450

Query: 427 LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH 486
           LSHAD+QMK+LPATAQVAAQQGAYLSRCFNRREHCTENPEGPR FKSSGRH FLPFRYKH
Sbjct: 451 LSHADTQMKNLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRHFKSSGRHQFLPFRYKH 510

Query: 487 LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR 546
           LGQFAPLGGEQAAAELPGD VSMG STQWLWYSVYASKQVSWRTR+LVVSDWTRKFIFGR
Sbjct: 511 LGQFAPLGGEQAAAELPGDWVSMGHSTQWLWYSVYASKQVSWRTRYLVVSDWTRKFIFGR 570

Query: 547 DSSRI 551
           DSSRI
Sbjct: 571 DSSRI 575

BLAST of MC04g0156 vs. NCBI nr
Match: KAG7011952.1 (External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1016 bits (2627), Expect = 0.0
Identity = 500/551 (90.74%), Postives = 527/551 (95.64%), Query Frame = 0

Query: 1   VVCLDCSSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVS 60
           +VC   SSGGLLAYA+S+S+  +PIEECPKKRVLVLGTGWAG SFLKDLDAS YDVQVVS
Sbjct: 1   MVCFGFSSGGLLAYADSQSEDSSPIEECPKKRVLVLGTGWAGTSFLKDLDASKYDVQVVS 60

Query: 61  PQNYFSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRS 120
           PQNYFSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGE+KFWEAEC KIDAANKKVFCRS
Sbjct: 61  PQNYFSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEVKFWEAECTKIDAANKKVFCRS 120

Query: 121 NVDNNLVGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFE 180
            VDNNLVGNREF LEYDYLVIAMGAQVNTFNTPGVKENCHFLKEV+DA KIR+SVIDCFE
Sbjct: 121 LVDNNLVGNREFALEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVQDAQKIRKSVIDCFE 180

Query: 181 MAVIPSLSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQS 240
           MAVIPSLSDEERR NLHFVIVGGGPTG+EFAAEL+DF +EDLV LYPTVKDLVKISVIQS
Sbjct: 181 MAVIPSLSDEERRRNLHFVIVGGGPTGVEFAAELHDFFKEDLVYLYPTVKDLVKISVIQS 240

Query: 241 GDHILNAFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIV 300
           GDHILNAFDERISSFAEQKFQRDGIDV+TGCRV+SVSDK+IKMKVKS+GE CSIPHGLIV
Sbjct: 241 GDHILNAFDERISSFAEQKFQRDGIDVHTGCRVISVSDKEIKMKVKSSGEVCSIPHGLIV 300

Query: 301 WSTGIMTRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDIST 360
           WSTGIMTRPVVKDFMEQIGQG RRIL TDEWL+VKGTQNVYAIGDCATIDQRKIM+DI+ 
Sbjct: 301 WSTGIMTRPVVKDFMEQIGQGSRRILTTDEWLRVKGTQNVYAIGDCATIDQRKIMKDIAA 360

Query: 361 IFKAADKDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDI 420
           IF+AADKD+SGTLTV EFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHE+ VDI
Sbjct: 361 IFEAADKDNSGTLTVTEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHEDEVDI 420

Query: 421 EGFKSALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFL 480
           EGFKSALSHAD+QMK+LPATAQVAAQQGAYLSRCFNRRE CTENPEGPR+FKSSGR+ FL
Sbjct: 421 EGFKSALSHADTQMKNLPATAQVAAQQGAYLSRCFNRREQCTENPEGPRRFKSSGRYQFL 480

Query: 481 PFRYKHLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTR 540
           PFRYKHLGQFAPLGGEQAAAELPGD VSMGRSTQWLWYSVYASKQVSWRTR+LVVSDWTR
Sbjct: 481 PFRYKHLGQFAPLGGEQAAAELPGDWVSMGRSTQWLWYSVYASKQVSWRTRYLVVSDWTR 540

Query: 541 KFIFGRDSSRI 551
           KF+FGRDSS I
Sbjct: 541 KFVFGRDSSGI 551

BLAST of MC04g0156 vs. ExPASy TrEMBL
Match: A0A6J1D1Y0 (NADH:ubiquinone reductase (non-electrogenic) OS=Momordica charantia OX=3673 GN=LOC111016845 PE=3 SV=1)

HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 7   SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 66
           SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS
Sbjct: 30  SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 89

Query: 67  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 126
           FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL
Sbjct: 90  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 149

Query: 127 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 186
           VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS
Sbjct: 150 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 209

Query: 187 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 246
           LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN
Sbjct: 210 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 269

Query: 247 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 306
           AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM
Sbjct: 270 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 329

Query: 307 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 366
           TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD
Sbjct: 330 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 389

Query: 367 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA 426
           KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA
Sbjct: 390 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA 449

Query: 427 LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH 486
           LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH
Sbjct: 450 LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH 509

Query: 487 LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR 546
           LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR
Sbjct: 510 LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR 569

Query: 547 DSSRI 551
           DSSRI
Sbjct: 570 DSSRI 574

BLAST of MC04g0156 vs. ExPASy TrEMBL
Match: A0A6J1D4A5 (NADH:ubiquinone reductase (non-electrogenic) OS=Momordica charantia OX=3673 GN=LOC111016845 PE=3 SV=1)

HSP 1 Score: 1097 bits (2836), Expect = 0.0
Identity = 544/544 (100.00%), Postives = 544/544 (100.00%), Query Frame = 0

Query: 8   SGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFSF 67
           SGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFSF
Sbjct: 9   SGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFSF 68

Query: 68  TPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNLV 127
           TPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNLV
Sbjct: 69  TPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNLV 128

Query: 128 GNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPSL 187
           GNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPSL
Sbjct: 129 GNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPSL 188

Query: 188 SDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILNA 247
           SDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILNA
Sbjct: 189 SDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILNA 248

Query: 248 FDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIMT 307
           FDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIMT
Sbjct: 249 FDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIMT 308

Query: 308 RPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAADK 367
           RPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAADK
Sbjct: 309 RPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAADK 368

Query: 368 DDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSAL 427
           DDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSAL
Sbjct: 369 DDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSAL 428

Query: 428 SHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKHL 487
           SHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKHL
Sbjct: 429 SHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKHL 488

Query: 488 GQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGRD 547
           GQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGRD
Sbjct: 489 GQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGRD 548

Query: 548 SSRI 551
           SSRI
Sbjct: 549 SSRI 552

BLAST of MC04g0156 vs. ExPASy TrEMBL
Match: A0A1S3C0A0 (NADH:ubiquinone reductase (non-electrogenic) OS=Cucumis melo OX=3656 GN=LOC103495538 PE=3 SV=1)

HSP 1 Score: 1016 bits (2628), Expect = 0.0
Identity = 500/545 (91.74%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 7   SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 66
           S GGLLAYA+S+SD G  IEE PKKRV+VLGTGWAG SFLKDLDAS YDVQVVSPQNYFS
Sbjct: 31  SGGGLLAYADSQSDVGGSIEESPKKRVVVLGTGWAGTSFLKDLDASKYDVQVVSPQNYFS 90

Query: 67  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 126
           FTPLLPSVTCG+VEARSIVEPVRNIVKKRKGEIKFWEAEC KIDAANKKVFC+S+VDNNL
Sbjct: 91  FTPLLPSVTCGSVEARSIVEPVRNIVKKRKGEIKFWEAECFKIDAANKKVFCQSHVDNNL 150

Query: 127 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 186
           VGNREF LEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDA KIRRSVIDCFEMAVIPS
Sbjct: 151 VGNREFSLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDAQKIRRSVIDCFEMAVIPS 210

Query: 187 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 246
           LS+EERR NLHFVIVGGGPTG+EFAAEL+DF EEDLVNLYP+VKDLVKISVIQSGDHILN
Sbjct: 211 LSEEERRRNLHFVIVGGGPTGVEFAAELHDFFEEDLVNLYPSVKDLVKISVIQSGDHILN 270

Query: 247 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 306
           AFDERIS FAEQKF RDGIDVYTGCRVVSVSDK+I+MKVKSTGE CS+PHGLIVWSTGIM
Sbjct: 271 AFDERISLFAEQKFLRDGIDVYTGCRVVSVSDKEIEMKVKSTGESCSMPHGLIVWSTGIM 330

Query: 307 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 366
           TRPVVKDFMEQIGQG RRILATDEWLQVKG QNVYAIGDCATIDQRKIMEDI+TIFKAAD
Sbjct: 331 TRPVVKDFMEQIGQGSRRILATDEWLQVKGAQNVYAIGDCATIDQRKIMEDIATIFKAAD 390

Query: 367 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA 426
           KD+SGTLTV EFQDV+DDILIRYPQVEI+LRSKHLRDVKDLL+DSQGHEN +DIEGFKSA
Sbjct: 391 KDNSGTLTVAEFQDVLDDILIRYPQVEIFLRSKHLRDVKDLLRDSQGHENEIDIEGFKSA 450

Query: 427 LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH 486
           LSHAD+QMK+LPATAQVAAQQGAYLSRCFNRREHCTENPEGPR FKSSGRH FLPFRYKH
Sbjct: 451 LSHADTQMKNLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRHFKSSGRHQFLPFRYKH 510

Query: 487 LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR 546
           LGQFAPLGGEQAAAELPGD VSMG STQWLWYSVYASKQVSWRTR+LVVSDWTRKFIFGR
Sbjct: 511 LGQFAPLGGEQAAAELPGDWVSMGHSTQWLWYSVYASKQVSWRTRYLVVSDWTRKFIFGR 570

Query: 547 DSSRI 551
           DSSRI
Sbjct: 571 DSSRI 575

BLAST of MC04g0156 vs. ExPASy TrEMBL
Match: A0A5A7SLV8 (NADH:ubiquinone reductase (non-electrogenic) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003680 PE=3 SV=1)

HSP 1 Score: 1015 bits (2624), Expect = 0.0
Identity = 499/545 (91.56%), Postives = 521/545 (95.60%), Query Frame = 0

Query: 7   SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 66
           S GGLLAYA+S+SD G  IEE PKKRV+VLGTGWAG SFLKDLDAS YDVQVVSPQNYFS
Sbjct: 31  SGGGLLAYADSQSDVGGSIEESPKKRVVVLGTGWAGTSFLKDLDASKYDVQVVSPQNYFS 90

Query: 67  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 126
           FTPLLPSVTCG+VEARSIVEPVRNIVKKRKGEIKFWEAEC KIDAANKKVFC+S+VDNNL
Sbjct: 91  FTPLLPSVTCGSVEARSIVEPVRNIVKKRKGEIKFWEAECFKIDAANKKVFCQSHVDNNL 150

Query: 127 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 186
           VGNREF LEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDA KIRRSVIDCFEMAVIPS
Sbjct: 151 VGNREFSLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDAQKIRRSVIDCFEMAVIPS 210

Query: 187 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 246
           LS+EERR NLHFVIVGGGPTG+EF AEL+DF EEDLVNLYP+VKDLVKISVIQSGDHILN
Sbjct: 211 LSEEERRRNLHFVIVGGGPTGVEFVAELHDFFEEDLVNLYPSVKDLVKISVIQSGDHILN 270

Query: 247 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 306
           AFDERIS FAEQKF RDGIDVYTGCRVVSVSDK+I+MKVKSTGE CS+PHGLIVWSTGIM
Sbjct: 271 AFDERISLFAEQKFLRDGIDVYTGCRVVSVSDKEIEMKVKSTGESCSMPHGLIVWSTGIM 330

Query: 307 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 366
           TRPVVKDFMEQIGQG RRILATDEWLQVKG QNVYAIGDCATIDQRKIMEDI+TIFKAAD
Sbjct: 331 TRPVVKDFMEQIGQGSRRILATDEWLQVKGAQNVYAIGDCATIDQRKIMEDIATIFKAAD 390

Query: 367 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA 426
           KD+SGTLTV EFQDV+DDILIRYPQVEI+LRSKHLRDVKDLL+DSQGHEN +DIEGFKSA
Sbjct: 391 KDNSGTLTVAEFQDVLDDILIRYPQVEIFLRSKHLRDVKDLLRDSQGHENEIDIEGFKSA 450

Query: 427 LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH 486
           LSHAD+QMK+LPATAQVAAQQGAYLSRCFNRREHCTENPEGPR FKSSGRH FLPFRYKH
Sbjct: 451 LSHADTQMKNLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRHFKSSGRHQFLPFRYKH 510

Query: 487 LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR 546
           LGQFAPLGGEQAAAELPGD VSMG STQWLWYSVYASKQVSWRTR+LVVSDWTRKFIFGR
Sbjct: 511 LGQFAPLGGEQAAAELPGDWVSMGHSTQWLWYSVYASKQVSWRTRYLVVSDWTRKFIFGR 570

Query: 547 DSSRI 551
           DSSRI
Sbjct: 571 DSSRI 575

BLAST of MC04g0156 vs. ExPASy TrEMBL
Match: A0A6J1GML5 (NADH:ubiquinone reductase (non-electrogenic) OS=Cucurbita moschata OX=3662 GN=LOC111455345 PE=3 SV=1)

HSP 1 Score: 1014 bits (2622), Expect = 0.0
Identity = 499/545 (91.56%), Postives = 524/545 (96.15%), Query Frame = 0

Query: 7   SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 66
           SSGGLLAYA+S+SD  +PIEECPKKRVLVLGTGWAG SFLKDLDAS YDVQVVSPQNYFS
Sbjct: 30  SSGGLLAYADSQSDASSPIEECPKKRVLVLGTGWAGTSFLKDLDASKYDVQVVSPQNYFS 89

Query: 67  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 126
           FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGE+KFWEAEC KIDAANKKVFCRS VDNNL
Sbjct: 90  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEVKFWEAECTKIDAANKKVFCRSLVDNNL 149

Query: 127 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 186
           VGNREF LEYDYLVIAMGAQVNTFNTPGVKENCHFLKEV+DA KIR+SVIDCFEMAVIPS
Sbjct: 150 VGNREFALEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVQDAQKIRKSVIDCFEMAVIPS 209

Query: 187 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 246
           LSDEERR NLHFVIVGGGPTG+EFAAEL+DF +EDLV LYPTVKDLVKISVIQSGDHILN
Sbjct: 210 LSDEERRRNLHFVIVGGGPTGVEFAAELHDFFKEDLVYLYPTVKDLVKISVIQSGDHILN 269

Query: 247 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 306
           AFDERISSFAEQKFQRDGIDV+TGCRV+SVSDK+IKMKVKS+GE CSIPHGLIVWSTGIM
Sbjct: 270 AFDERISSFAEQKFQRDGIDVHTGCRVISVSDKEIKMKVKSSGEVCSIPHGLIVWSTGIM 329

Query: 307 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 366
           TRPVVKDFMEQIGQG RRIL TDEWL+VKGTQNVYAIGDCATIDQRKIM+DI+ IF+AAD
Sbjct: 330 TRPVVKDFMEQIGQGSRRILTTDEWLRVKGTQNVYAIGDCATIDQRKIMKDIAAIFEAAD 389

Query: 367 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENGVDIEGFKSA 426
           KD+SGTLTV EFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHE+ VDIEGFKSA
Sbjct: 390 KDNSGTLTVTEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHEDEVDIEGFKSA 449

Query: 427 LSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYKH 486
           LSHAD+QMK+LPATAQVAAQQGAYLSRCFNRRE CTENPEGPR+FKSSGR+ FLPFRYKH
Sbjct: 450 LSHADTQMKNLPATAQVAAQQGAYLSRCFNRREQCTENPEGPRRFKSSGRYQFLPFRYKH 509

Query: 487 LGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGR 546
           LGQFAPLGGEQAAAELPGD VSMGRSTQWLWYSVYASKQVSWRTR+LVVSDWTRKF+FGR
Sbjct: 510 LGQFAPLGGEQAAAELPGDWVSMGRSTQWLWYSVYASKQVSWRTRYLVVSDWTRKFVFGR 569

Query: 547 DSSRI 551
           DSS I
Sbjct: 570 DSSGI 574

BLAST of MC04g0156 vs. TAIR 10
Match: AT4G28220.1 (NAD(P)H dehydrogenase B1 )

HSP 1 Score: 818.9 bits (2114), Expect = 2.4e-237
Identity = 389/546 (71.25%), Postives = 465/546 (85.16%), Query Frame = 0

Query: 7   SSGGLLAYAESRSDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNYFS 66
           S G ++AYA++ ++  N  EE  KK+V+VLGTGWAGISFLKDLD + YDVQVVSPQNYF+
Sbjct: 27  SGGSIVAYADA-NEEANKKEEHKKKKVVVLGTGWAGISFLKDLDITSYDVQVVSPQNYFA 86

Query: 67  FTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDNNL 126
           FTPLLPSVTCGTVEARSIVE VRNI KK+ GEI+ WEA+C KID  N+KV CR    ++ 
Sbjct: 87  FTPLLPSVTCGTVEARSIVESVRNITKKKNGEIELWEADCFKIDHVNQKVHCRPVFKDDP 146

Query: 127 VGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVIPS 186
             ++EF L YDYL++A+GAQVNTF TPGV ENCHFLKEVEDA +IRR VIDCFE A++P 
Sbjct: 147 EASQEFSLGYDYLIVAVGAQVNTFGTPGVLENCHFLKEVEDAQRIRRGVIDCFEKAILPG 206

Query: 187 LSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHILN 246
           L++E+RR  LHFVIVGGGPTG+EFAAEL+DF+ ED+  +YP+VK+LVKI++IQSGDHILN
Sbjct: 207 LTEEQRRRKLHFVIVGGGPTGVEFAAELHDFIIEDITKIYPSVKELVKITLIQSGDHILN 266

Query: 247 AFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTGIM 306
            FDERISSFAEQKF RDGIDV TG RV+SV+DKDI +KVKS+GE  SIPHGLI+WSTG+ 
Sbjct: 267 TFDERISSFAEQKFTRDGIDVQTGMRVMSVTDKDITVKVKSSGELVSIPHGLILWSTGVG 326

Query: 307 TRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKAAD 366
           TRPV+ DFMEQ+GQG RR +AT+EWLQV G +NVYA+GDCA+I QRKI+ DI+ IFKAAD
Sbjct: 327 TRPVISDFMEQVGQGGRRAVATNEWLQVTGCENVYAVGDCASIAQRKILGDIANIFKAAD 386

Query: 367 KDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGH-ENGVDIEGFKS 426
            D+SGTLT++E + V+DDI++RYPQVE+YL+SKH+R + DLL DS+G+    VDIE FK 
Sbjct: 387 ADNSGTLTMEELEGVVDDIIVRYPQVELYLKSKHMRHINDLLADSEGNARKEVDIEAFKL 446

Query: 427 ALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHLFLPFRYK 486
           ALS ADSQMK+LPATAQVAAQQGAYL++CFNR E C E PEGP++F++ G H F PF+YK
Sbjct: 447 ALSEADSQMKTLPATAQVAAQQGAYLAKCFNRMEQCKELPEGPKRFRTGGHHQFRPFQYK 506

Query: 487 HLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFG 546
           H GQFAPLGG+QAAAELPGD VS G+S QWLWYSVYASKQVSWRTR LVVSDWTR++IFG
Sbjct: 507 HFGQFAPLGGDQAAAELPGDWVSAGKSAQWLWYSVYASKQVSWRTRALVVSDWTRRYIFG 566

Query: 547 RDSSRI 552
           RDSSRI
Sbjct: 567 RDSSRI 571

BLAST of MC04g0156 vs. TAIR 10
Match: AT4G05020.1 (NAD(P)H dehydrogenase B2 )

HSP 1 Score: 748.0 bits (1930), Expect = 5.2e-216
Identity = 362/555 (65.23%), Postives = 448/555 (80.72%), Query Frame = 0

Query: 7   SSGGLLAYAESRSD---TGNPIEE--CPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSP 66
           S GGL+AY+E+ +     G  + E    KK+V++LGTGWAG SFLK+L+ S Y+VQ++SP
Sbjct: 30  SGGGLIAYSEANASYGANGGAVVETGTKKKKVVLLGTGWAGTSFLKNLNNSQYEVQIISP 89

Query: 67  QNYFSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSN 126
           +NYF+FTPLLPSVTCGTVEARS+VEP+RNI +K   +  + EAEC KID A+KKV+CRS 
Sbjct: 90  RNYFAFTPLLPSVTCGTVEARSVVEPIRNIGRKNV-DTSYLEAECFKIDPASKKVYCRSK 149

Query: 127 VDNNLVGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEM 186
              +  G +EF ++YDYLVIA GAQ NTFN PGV+ENCHFLKEVEDA +IR++VID FE 
Sbjct: 150 QGLSSNGKKEFSVDYDYLVIATGAQSNTFNIPGVEENCHFLKEVEDAQRIRKTVIDSFEK 209

Query: 187 AVIPSLSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSG 246
           A +P LSDEER+  LHFV+VGGGPTG+EFAAEL+DF+ EDLV+LYP  K  V+I+++++ 
Sbjct: 210 ASLPELSDEERKRILHFVVVGGGPTGVEFAAELHDFVTEDLVSLYPRAKGSVRITLLEAA 269

Query: 247 DHILNAFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVW 306
           DHIL  FD+RI+ FAE+KF RDGIDV  G  V  V++KDI  K K  GE  SIP+G+IVW
Sbjct: 270 DHILTMFDKRITEFAEEKFSRDGIDVKLGSMVTKVNEKDISAKTKG-GEVSSIPYGMIVW 329

Query: 307 STGIMTRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTI 366
           STGI TRPV+KDFM+QIGQG RR LATDEWL+V+GT N+YA+GDCATI+QRK+MED+S I
Sbjct: 330 STGIGTRPVIKDFMKQIGQGNRRALATDEWLRVEGTDNIYALGDCATINQRKVMEDVSAI 389

Query: 367 FKAADKDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHE---NGV 426
           F  ADKD SGTLT+KEFQ+ +DDI +RYPQVE+YL+SK +R + DLLK+++  +   N +
Sbjct: 390 FSKADKDKSGTLTLKEFQEAMDDICVRYPQVELYLKSKRMRGIADLLKEAETDDVSKNNI 449

Query: 427 D--IEGFKSALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGR 486
           +  IE FKSALS  DSQ+K LPATAQVAAQQGAYL++CF+R E C ++PEGP + +  GR
Sbjct: 450 ELKIEEFKSALSQVDSQVKFLPATAQVAAQQGAYLAKCFDRMEECEKSPEGPIRMRGEGR 509

Query: 487 HLFLPFRYKHLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVS 546
           H F PFRY+HLGQFAPLGGEQ AA+LPGD VS+G S+QWLWYSVYASKQVSWRTR LVVS
Sbjct: 510 HRFRPFRYRHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRVLVVS 569

Query: 547 DWTRKFIFGRDSSRI 552
           DW R+FIFGRDSS I
Sbjct: 570 DWMRRFIFGRDSSSI 582

BLAST of MC04g0156 vs. TAIR 10
Match: AT4G21490.1 (NAD(P)H dehydrogenase B3 )

HSP 1 Score: 747.7 bits (1929), Expect = 6.8e-216
Identity = 361/553 (65.28%), Postives = 447/553 (80.83%), Query Frame = 0

Query: 7   SSGGLLAYAESR-SDTGNPIE-ECPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSPQNY 66
           S GGL+ Y+E+  S + N +E +  K++V++LGTGWAG SFLK L+ S Y+VQV+SP+NY
Sbjct: 30  SGGGLIVYSEANPSYSNNGVETKTRKRKVVLLGTGWAGASFLKTLNNSSYEVQVISPRNY 89

Query: 67  FSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSNVDN 126
           F+FTPLLPSVTCGTVEARS+VEP+RNI +K+  E+ F EAEC KID  +KKV+CRS    
Sbjct: 90  FAFTPLLPSVTCGTVEARSVVEPIRNIARKQNVEMSFLEAECFKIDPGSKKVYCRSKQGV 149

Query: 127 NLVGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEMAVI 186
           N  G +EFD++YDYLVIA GAQ NTFN PGV+ENCHFLKEVEDA +IR +VID FE A +
Sbjct: 150 NSKGKKEFDVDYDYLVIATGAQSNTFNIPGVEENCHFLKEVEDAQRIRSTVIDSFEKASL 209

Query: 187 PSLSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSGDHI 246
           P L+++ER+  LHFV+VGGGPTG+EFA+EL+DF+ EDLV LYP  K+LV+I+++++ DHI
Sbjct: 210 PGLNEQERKRMLHFVVVGGGPTGVEFASELHDFVNEDLVKLYPKAKNLVQITLLEAADHI 269

Query: 247 LNAFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVWSTG 306
           L  FD+RI+ FAE+KF RDGIDV  G  VV V+DK+I  K K+ GE  +IP+G+IVWSTG
Sbjct: 270 LTMFDKRITEFAEEKFTRDGIDVKLGSMVVKVNDKEISAKTKA-GEVSTIPYGMIVWSTG 329

Query: 307 IMTRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTIFKA 366
           I TRPV+KDFM+QIGQG RR LATDEWL+V+G  N+YA+GDCATI+QRK+MEDI+ IFK 
Sbjct: 330 IGTRPVIKDFMKQIGQGNRRALATDEWLRVEGCDNIYALGDCATINQRKVMEDIAAIFKK 389

Query: 367 ADKDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHENG------V 426
           ADK++SGTLT+KEF +V+ DI  RYPQVE+YL+SK +  + DLLK +Q  ENG      +
Sbjct: 390 ADKENSGTLTMKEFHEVMSDICDRYPQVELYLKSKGMHGITDLLKQAQA-ENGSNKSVEL 449

Query: 427 DIEGFKSALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGRHL 486
           DIE  KSAL   DSQ+K LPAT QVAAQQG YL++CF+R E C +NPEGP + +  GRH 
Sbjct: 450 DIEELKSALCQVDSQVKLLPATGQVAAQQGTYLAKCFDRMEVCEKNPEGPIRIRGEGRHR 509

Query: 487 FLPFRYKHLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDW 546
           F PFRY+HLGQFAPLGGEQ AA+LPGD VS+G S+QWLWYSVYASKQVSWRTR LVVSDW
Sbjct: 510 FRPFRYRHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRVLVVSDW 569

Query: 547 TRKFIFGRDSSRI 552
            R+FIFGRDSSRI
Sbjct: 570 MRRFIFGRDSSRI 580

BLAST of MC04g0156 vs. TAIR 10
Match: AT4G05020.2 (NAD(P)H dehydrogenase B2 )

HSP 1 Score: 729.6 bits (1882), Expect = 1.9e-210
Identity = 362/592 (61.15%), Postives = 448/592 (75.68%), Query Frame = 0

Query: 7   SSGGLLAYAESRSD---TGNPIEE--CPKKRVLVLGTGWAGISFLKDLDASIYDVQVVSP 66
           S GGL+AY+E+ +     G  + E    KK+V++LGTGWAG SFLK+L+ S Y+VQ++SP
Sbjct: 30  SGGGLIAYSEANASYGANGGAVVETGTKKKKVVLLGTGWAGTSFLKNLNNSQYEVQIISP 89

Query: 67  QNYFSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKVFCRSN 126
           +NYF+FTPLLPSVTCGTVEARS+VEP+RNI +K   +  + EAEC KID A+KKV+CRS 
Sbjct: 90  RNYFAFTPLLPSVTCGTVEARSVVEPIRNIGRKNV-DTSYLEAECFKIDPASKKVYCRSK 149

Query: 127 VDNNLVGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVIDCFEM 186
              +  G +EF ++YDYLVIA GAQ NTFN PGV+ENCHFLKEVEDA +IR++VID FE 
Sbjct: 150 QGLSSNGKKEFSVDYDYLVIATGAQSNTFNIPGVEENCHFLKEVEDAQRIRKTVIDSFEK 209

Query: 187 AVIPSLSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKISVIQSG 246
           A +P LSDEER+  LHFV+VGGGPTG+EFAAEL+DF+ EDLV+LYP  K  V+I+++++ 
Sbjct: 210 ASLPELSDEERKRILHFVVVGGGPTGVEFAAELHDFVTEDLVSLYPRAKGSVRITLLEAA 269

Query: 247 DHILNAFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPHGLIVW 306
           DHIL  FD+RI+ FAE+KF RDGIDV  G  V  V++KDI  K K  GE  SIP+G+IVW
Sbjct: 270 DHILTMFDKRITEFAEEKFSRDGIDVKLGSMVTKVNEKDISAKTKG-GEVSSIPYGMIVW 329

Query: 307 STGIMTRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIMEDISTI 366
           STGI TRPV+KDFM+QIGQG RR LATDEWL+V+GT N+YA+GDCATI+QRK+MED+S I
Sbjct: 330 STGIGTRPVIKDFMKQIGQGNRRALATDEWLRVEGTDNIYALGDCATINQRKVMEDVSAI 389

Query: 367 FKAADKDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHE---NGV 426
           F  ADKD SGTLT+KEFQ+ +DDI +RYPQVE+YL+SK +R + DLLK+++  +   N +
Sbjct: 390 FSKADKDKSGTLTLKEFQEAMDDICVRYPQVELYLKSKRMRGIADLLKEAETDDVSKNNI 449

Query: 427 D--IEGFKSALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGR 486
           +  IE FKSALS  DSQ+K LPATAQVAAQQGAYL++CF+R E C ++PEGP + +  GR
Sbjct: 450 ELKIEEFKSALSQVDSQVKFLPATAQVAAQQGAYLAKCFDRMEECEKSPEGPIRMRGEGR 509

Query: 487 HLFLPF-------------------------------------RYKHLGQFAPLGGEQAA 546
           H F PF                                     RY+HLGQFAPLGGEQ A
Sbjct: 510 HRFRPFRIWKLRGISKTLSQDTTESCALDHKGVGKMELIPYIIRYRHLGQFAPLGGEQTA 569

Query: 547 AELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVSDWTRKFIFGRDSSRI 552
           A+LPGD VS+G S+QWLWYSVYASKQVSWRTR LVVSDW R+FIFGRDSS I
Sbjct: 570 AQLPGDWVSIGHSSQWLWYSVYASKQVSWRTRVLVVSDWMRRFIFGRDSSSI 619

BLAST of MC04g0156 vs. TAIR 10
Match: AT2G20800.1 (NAD(P)H dehydrogenase B4 )

HSP 1 Score: 713.0 bits (1839), Expect = 1.9e-205
Identity = 337/555 (60.72%), Postives = 437/555 (78.74%), Query Frame = 0

Query: 9   GGLLAYAESR------------SDTGNPIEECPKKRVLVLGTGWAGISFLKDLDASIYDV 68
           GG+L Y++S                GNPI    KK+V+VLG+GW+G SFL  L+   YDV
Sbjct: 33  GGVLVYSDSNPLKRILHADATLDSDGNPIR---KKKVVVLGSGWSGYSFLSYLNNPNYDV 92

Query: 69  QVVSPQNYFSFTPLLPSVTCGTVEARSIVEPVRNIVKKRKGEIKFWEAECIKIDAANKKV 128
           QVVSP+N+F FTPLLPSVT GTVEARSIVEP+R +++K+  E K  EAEC+KIDA+NKK+
Sbjct: 93  QVVSPRNFFLFTPLLPSVTNGTVEARSIVEPIRGLMRKKGFEYK--EAECVKIDASNKKI 152

Query: 129 FCRSNVDNNLVGNREFDLEYDYLVIAMGAQVNTFNTPGVKENCHFLKEVEDALKIRRSVI 188
            CRS   ++L G  EFD++YD L++A+GA+ NTFNTPGV+E+ +FLKE EDAL IR SVI
Sbjct: 153 HCRSKEGSSLKGTTEFDMDYDILILAVGAKPNTFNTPGVEEHAYFLKEAEDALNIRHSVI 212

Query: 189 DCFEMAVIPSLSDEERRENLHFVIVGGGPTGIEFAAELNDFLEEDLVNLYPTVKDLVKIS 248
           DCFE A +P+L++EER++ LHFV+VGGGPTG+EF+AEL+DFL +D+  +YP V++  KI+
Sbjct: 213 DCFERASLPNLTEEERKKILHFVVVGGGPTGVEFSAELHDFLVQDVAKIYPKVQEFTKIT 272

Query: 249 VIQSGDHILNAFDERISSFAEQKFQRDGIDVYTGCRVVSVSDKDIKMKVKSTGEFCSIPH 308
           ++++GDHILN FD+RI++FAE+KFQRDGID+ TG  VV V+  +I  K + TG+  S P+
Sbjct: 273 LLEAGDHILNMFDKRITAFAEEKFQRDGIDLKTGSMVVGVTADEISTKERETGKIVSEPY 332

Query: 309 GLIVWSTGIMTRPVVKDFMEQIGQGKRRILATDEWLQVKGTQNVYAIGDCATIDQRKIME 368
           G++VWSTGI +RPV+KDFM+QIGQG+RR+LATDEWL+V+G   VYA+GD ATI+QR++ME
Sbjct: 333 GMVVWSTGIGSRPVIKDFMQQIGQGQRRVLATDEWLRVEGCDGVYALGDTATINQRRVME 392

Query: 369 DISTIFKAADKDDSGTLTVKEFQDVIDDILIRYPQVEIYLRSKHLRDVKDLLKDSQGHEN 428
           DI+ IF  ADK ++GTL  K+F  V+ DI  RYPQVE+YL+   L+++ +LLK + G + 
Sbjct: 393 DIAAIFNKADKGNTGTLKKKDFNSVVKDICQRYPQVELYLKKNKLKNIANLLKSANGEDT 452

Query: 429 GVDIEGFKSALSHADSQMKSLPATAQVAAQQGAYLSRCFNRREHCTENPEGPRQFKSSGR 488
            V+IE FK ALS  DSQMK+LPATAQVA+QQG YL++CFN+ E C + PEGP +F+  GR
Sbjct: 453 QVNIEKFKQALSEVDSQMKNLPATAQVASQQGKYLAKCFNKMEKCEKKPEGPLRFRGEGR 512

Query: 489 HLFLPFRYKHLGQFAPLGGEQAAAELPGDLVSMGRSTQWLWYSVYASKQVSWRTRFLVVS 548
           H F PFRY+H G FAPLGGEQ AAELPGD VS+G S+QWLWYSVYASK VSWRTR LV+S
Sbjct: 513 HRFQPFRYRHFGSFAPLGGEQTAAELPGDWVSIGHSSQWLWYSVYASKLVSWRTRMLVIS 572

Query: 549 DWTRKFIFGRDSSRI 552
           DWTR+F+FGRDSS I
Sbjct: 573 DWTRRFVFGRDSSSI 582

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
M1BYJ71.4e-25374.87External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial OS=Sola... [more]
Q9ST624.0e-25374.51External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial OS=Sola... [more]
Q1JPL43.4e-23671.25External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial OS=Arab... [more]
Q94BV77.4e-21565.23External alternative NAD(P)H-ubiquinone oxidoreductase B2, mitochondrial OS=Arab... [more]
F4JJJ39.6e-21565.28External alternative NAD(P)H-ubiquinone oxidoreductase B3, mitochondrial OS=Arab... [more]
Match NameE-valueIdentityDescription
XP_022148080.10.0100.00external alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial isoform... [more]
XP_022148081.10.0100.00external alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial isoform... [more]
XP_038888314.10.092.29external alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial-like [B... [more]
XP_008455351.10.091.74PREDICTED: external alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondr... [more]
KAG7011952.10.090.74External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial [Cucurb... [more]
Match NameE-valueIdentityDescription
A0A6J1D1Y00.0100.00NADH:ubiquinone reductase (non-electrogenic) OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1D4A50.0100.00NADH:ubiquinone reductase (non-electrogenic) OS=Momordica charantia OX=3673 GN=L... [more]
A0A1S3C0A00.091.74NADH:ubiquinone reductase (non-electrogenic) OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5A7SLV80.091.56NADH:ubiquinone reductase (non-electrogenic) OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1GML50.091.56NADH:ubiquinone reductase (non-electrogenic) OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT4G28220.12.4e-23771.25NAD(P)H dehydrogenase B1 [more]
AT4G05020.15.2e-21665.23NAD(P)H dehydrogenase B2 [more]
AT4G21490.16.8e-21665.28NAD(P)H dehydrogenase B3 [more]
AT4G05020.21.9e-21061.15NAD(P)H dehydrogenase B2 [more]
AT2G20800.11.9e-20560.72NAD(P)H dehydrogenase B4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00368FADPNRcoord: 33..52
score: 27.09
coord: 136..154
score: 24.84
coord: 197..215
score: 37.44
NoneNo IPR availableGENE3D3.50.50.100coord: 401..550
e-value: 1.1E-28
score: 102.2
coord: 24..377
e-value: 1.2E-132
score: 444.9
NoneNo IPR availablePANTHERPTHR43706:SF29EXTERNAL ALTERNATIVE NAD(P)H-UBIQUINONE OXIDOREDUCTASE B1, MITOCHONDRIALcoord: 27..551
IPR023753FAD/NAD(P)-binding domainPFAMPF07992Pyr_redox_2coord: 31..355
e-value: 3.1E-57
score: 194.1
IPR045024Alternative NADH dehydrogenasePANTHERPTHR43706NADH DEHYDROGENASEcoord: 27..551
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 366..378
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 353..388
score: 12.114401
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 306..430
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 30..249
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 196..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0156.1MC04g0156.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006116 NADH oxidation
cellular_component GO:0005743 mitochondrial inner membrane
cellular_component GO:0005777 peroxisome
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0050136 NADH dehydrogenase (quinone) activity
molecular_function GO:0003954 NADH dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity