MC04g0131 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0131
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Diacylglycerol O-acyltransferase
Location	MC04: 982522 .. 983096 (+)
RNA-Seq Expression	MC04g0131
Synteny	MC04g0131

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGTGAAAGATACAAACGGGGAGAGAAAATGGAAGAAAGTTGAAGTGGATCCAGAAGAGCATGTTTTCGTGCCAACTTTTCCATCAAATTGGTCGCTTGAATTTGAACTCTTTGATGCATATTTCGACGAGTATATGACAGATTTGGCTCTACAACAGCTACCCCAAAATAGACCATTGTGGGAAATTCATGTAATTCAGTATCCCTCGAGTAATGTAGCTGGAATCATAGTTTTCAAGTTTCACCACTCACTTGGCGATGGCTATTCTCTTATGAGTCTTCTGTTTACTTGCTTTAAAAGGACAGATGATCCTTCATTCCATTAACATTTCCTTCGAGGAAATTCTCTGAGAAATCATCAGGCCATATTCTAAATCGCATGTCTCGAATTTTGTCTTCGGCTATGAACAGCGTAACGGATTTTGGATGGAGCATAATCAAAAGCAGTGTTCTTGAAGATGACTTGACACCCATAAGATCGGGTGGTGATCATGGAGTGCAGTTTCGGCCAATTGCAATTTGGTCAATATCATTCTCTCTTCATCAACTCAAACAAATCAAATCCAAACTTAAA

mRNA sequence

CAGGTGAAAGATACAAACGGGGAGAGAAAATGGAAGAAAGTTGAAGTGGATCCAGAAGAGCATGTTTTCGTGCCAACTTTTCCATCAAATTGGTCGCTTGAATTTGAACTCTTTGATGCATATTTCGACGAGTATATGACAGATTTGGCTCTACAACAGCTACCCCAAAATAGACCATTGTGGGAAATTCATGTAATTCAGTATCCCTCGAGTAATGTAGCTGGAATCATAGTTTTCAAGTTTCACCACTCACTTGGCGATGGCTATTCTCTTATGAGTCTTCTGTTTACTTGCTTTAAAAGGACAGATGATCCTTCACCATTAACATTTCCTTCGAGGAAATTCTCTGAGAAATCATCAGGCCATATTCTAAATCGCATGTCTCGAATTTTGTCTTCGGCTATGAACAGCGTAACGGATTTTGGATGGAGCATAATCAAAAGCAGTGTTCTTGAAGATGACTTGACACCCATAAGATCGGGTGGTGATCATGGAGTGCAGTTTCGGCCAATTGCAATTTGGTCAATATCATTCTCTCTTCATCAACTCAAACAAATCAAATCCAAACTTAAA

Coding sequence (CDS)

CAGGTGAAAGATACAAACGGGGAGAGAAAATGGAAGAAAGTTGAAGTGGATCCAGAAGAGCATGTTTTCGTGCCAACTTTTCCATCAAATTGGTCGCTTGAATTTGAACTCTTTGATGCATATTTCGACGAGTATATGACAGATTTGGCTCTACAACAGCTACCCCAAAATAGACCATTGTGGGAAATTCATGTAATTCAGTATCCCTCGAGTAATGTAGCTGGAATCATAGTTTTCAAGTTTCACCACTCACTTGGCGATGGCTATTCTCTTATGAGTCTTCTGTTTACTTGCTTTAAAAGGACAGATGATCCTTCACCATTAACATTTCCTTCGAGGAAATTCTCTGAGAAATCATCAGGCCATATTCTAAATCGCATGTCTCGAATTTTGTCTTCGGCTATGAACAGCGTAACGGATTTTGGATGGAGCATAATCAAAAGCAGTGTTCTTGAAGATGACTTGACACCCATAAGATCGGGTGGTGATCATGGAGTGCAGTTTCGGCCAATTGCAATTTGGTCAATATCATTCTCTCTTCATCAACTCAAACAAATCAAATCCAAACTTAAA

Protein sequence

QVKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLWEIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPSPLTFPSRKFSEKSSGHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSLHQLKQIKSKLK

Homology

[Close]

BLAST of MC04g0131 vs. ExPASy Swiss-Prot
Match: F4IU14 (Wax ester synthase/diacylglycerol acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=WSD3 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.4e-18
Identity = 57/189 (30.16%), Postives = 94/189 (49.74%), Query Frame = 0


Query: 6   NGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLWEIHV 65
           NG+  W  V V  E+HV VP    +     E  D + ++Y + LA   +  +RPLWE+H+
Sbjct: 77  NGKASWVPVSVRVEDHVVVPDLDYS---NIENPDQFIEDYTSKLANTPMDMSRPLWELHL 136

Query: 66  IQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS--PLTFPSRKF--SEKSSG 125
           +   +SN   + + KFHHSLGDG SL+SLL    ++T DP   P T  +RK   S K S 
Sbjct: 137 LNIKTSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASSNKKSW 196

Query: 126 HILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSLH 185
            ++ R   ++     +V +    ++    + D  TP+       ++ R +    +SF   
Sbjct: 197 WLVGRFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPLMGKTGDAIRSRKVIHRIVSFD-- 256

Query: 186 QLKQIKSKL 191
            +K +K+ +
Sbjct: 257 DVKLVKNNM 260

[Close]

BLAST of MC04g0131 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 6.4e-17
Identity = 59/205 (28.78%), Postives = 101/205 (49.27%), Query Frame = 0


Query: 2   VKDTNGE-RKWKKVEVDPEEHVFVPTF-PSNWSLEFELFDAYFDEYMTDLALQQLPQNRP 61
           V D NG+ ++W +  V  E+H  VP   P N     E  +A+ ++Y++DL    L  +RP
Sbjct: 70  VTDENGQNQRWVRTNVVVEDHFIVPKIKPQN----IENSNAFLEDYVSDLMKIPLDTSRP 129

Query: 62  LWEIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPSPLTFPSRKFSEKS 121
           LWE+H++   +S+   + V K HHS+GDG S+MSL+  C ++T +P  L     ++   S
Sbjct: 130 LWELHLLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSS 189

Query: 122 SGHILNRMSRILSS--------------AMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHG 181
              +L   SR  S                +N+V D    I+ +  ++D  TPI+ G    
Sbjct: 190 RSSLLTTGSRSDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIK-GDFLS 249

Query: 182 VQFRPIAIWSISFSLHQLKQIKSKL 191
            + + + +   + SL  +K  K+ +
Sbjct: 250 TKSKQLRLVHRTVSLDDIKLTKNAM 269

[Close]

BLAST of MC04g0131 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 6.4e-17
Identity = 62/199 (31.16%), Postives = 106/199 (53.27%), Query Frame = 0


Query: 8   ERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLWEIHVIQ 67
           E+KW +  V  E+HV +P   +   +E    D + + Y++DL    L  ++PLWE+H++ 
Sbjct: 87  EQKWVRTNVVVEDHVIIPKIQTQ-HIENANADVFLESYVSDLTTIPLDTSKPLWEVHLLD 146

Query: 68  YPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPSPL-TFP------SRKFSEKSS 127
             +S+   + V + HHSLGDG S+MSL+  C ++T +P+ L + P      S   S K+S
Sbjct: 147 LKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSGSSSLKTS 206

Query: 128 GHILNR---MSRILSSA----MNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGV-QFRPIA 187
               +R   +  +L SA    +N+V D    I  +  L+D  TPI+  GD  + + + + 
Sbjct: 207 SRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIK--GDFKLSKGKRMC 266

Query: 188 IWSISFSLHQLKQIKSKLK 192
           +   + SL  +K IK+ +K
Sbjct: 267 MVHRTVSLDDIKLIKNAMK 282

[Close]

BLAST of MC04g0131 vs. ExPASy Swiss-Prot
Match: Q9C7H4 (Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=WSD2 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.1e-16
Identity = 41/106 (38.68%), Postives = 65/106 (61.32%), Query Frame = 0


Query: 7   GERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLWEIHVI 66
           G+ +W   +++ EEHV VP    N     E  D + ++Y +++AL  +  ++PLWE H++
Sbjct: 72  GKARWIPTKINVEEHVIVPDIDPN----IENPDEFLEDYTSNMALSPMDMSKPLWEFHLL 131

Query: 67  QYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPSPLTFPS 113
           +  +S+   + V +FHHSLGDG SLMSLL  C ++T DP    FP+
Sbjct: 132 KLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPE--AFPT 171

[Close]

BLAST of MC04g0131 vs. ExPASy Swiss-Prot
Match: Q9FK04 (Wax ester synthase/diacylglycerol acyltransferase 10 OS=Arabidopsis thaliana OX=3702 GN=WSD10 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.4e-16
Identity = 62/197 (31.47%), Postives = 97/197 (49.24%), Query Frame = 0


Query: 6   NGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLWEIHV 65
           +GER W K +V+ E+HVFVP        +    D + D+Y++ L L  L +++PLW+IH+
Sbjct: 69  DGER-WMKTKVNVEDHVFVPDIDLQEINKDG--DGFVDDYVSRLTLSPLDKSKPLWDIHI 128

Query: 66  IQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDD-PSPLTFPSRKFSEKSSGH-- 125
           +   +S+   + V + HHSL DG SLMSLL  C ++T +  S  T P+ K  E+   H  
Sbjct: 129 LNVKTSDAEAVGVMRCHHSLADGMSLMSLLVACTRKTSNLESFPTIPAIKRREQMMSHRF 188

Query: 126 --------ILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIW 185
                    +N +   +    N++ D       S   +D  TPI  G   G   R    +
Sbjct: 189 GNKGWYSRSINAVYYAVRLIWNTIVDLLLLWATSLFFKDTETPISEGIGSGNNAR--RFY 248

Query: 186 SISFSLHQLKQIKSKLK 192
             + SL  +K IK+ +K
Sbjct: 249 HRTVSLDDIKLIKNAMK 260

[Close]

BLAST of MC04g0131 vs. NCBI nr
Match: XP_038888791.1 (O-acyltransferase WSD1-like [Benincasa hispida])

HSP 1 Score: 253 bits (647), Expect = 3.77e-79
Identity = 125/194 (64.43%), Postives = 155/194 (79.90%), Query Frame = 0


Query: 2   VKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLW 61
           V+D NG+RKWKKVEV+ EEH+ VPT PSN SL    +D YF EYMTDL +Q+LP+N+PLW
Sbjct: 64  VEDINGKRKWKKVEVNLEEHILVPTLPSNLSLVELNYDTYFKEYMTDLGVQELPKNKPLW 123

Query: 62  EIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS-PLTFPSRKFSEK-- 121
           EIH+I YP+ N A  ++ KFHHSLGDGYS+M LL +  KR D+PS PLTFPSRK +    
Sbjct: 124 EIHIINYPTKNAAAHLILKFHHSLGDGYSMMGLLISSMKRADNPSLPLTFPSRKINSDHN 183

Query: 122 -SSGHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSIS 181
             S ++L+ + + + S+MNSV DFGWSI+KSSVLEDDLTPIRSGGD GV+FRPIA W+I+
Sbjct: 184 LQSRNVLSLVPKFVLSSMNSVLDFGWSIMKSSVLEDDLTPIRSGGD-GVEFRPIATWTIN 243

Query: 182 FSLHQLKQIKSKLK 191
           FSLHQ+KQIKSKL+
Sbjct: 244 FSLHQIKQIKSKLR 256

[Close]

BLAST of MC04g0131 vs. NCBI nr
Match: KAE8645785.1 (hypothetical protein Csa_017199 [Cucumis sativus])

HSP 1 Score: 246 bits (628), Expect = 6.57e-79
Identity = 127/197 (64.47%), Postives = 157/197 (79.70%), Query Frame = 0


Query: 2   VKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFEL-FDAYFDEYMTDLALQQLPQNRPL 61
           V D NGERKWKKVEV+ EEH+ VPTFPSN S+  ++ +DAYF++Y+T+L  Q+L +N+PL
Sbjct: 66  VDDINGERKWKKVEVNLEEHILVPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPL 125

Query: 62  WEIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS-PLTFPSRKFSE-- 121
           WEIH+I+YP+SN A  ++ KFHHSLGDGYS+M LL +  KR D+PS PLTFPSRK +   
Sbjct: 126 WEIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDD 185

Query: 122 ---KSSGHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIW 181
              K+  H+LN     + S MNSV DFGWSIIKSSVLEDDLTPIRSGGD GV+FRP+AI 
Sbjct: 186 HNNKNLRHVLN----YVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGGD-GVEFRPVAIC 245

Query: 182 SISFSLHQLKQIKSKLK 191
           +ISFSLHQ+KQIKSKL+
Sbjct: 246 TISFSLHQIKQIKSKLR 257

[Close]

BLAST of MC04g0131 vs. NCBI nr
Match: XP_022952996.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 240 bits (613), Expect = 4.06e-74
Identity = 124/190 (65.26%), Postives = 151/190 (79.47%), Query Frame = 0


Query: 2   VKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLW 61
           V D NGERKWKKVEV+ EEH+ V T PSN SL+  LFDAYF+EYMT+ ALQ+LPQN+PLW
Sbjct: 62  VNDVNGERKWKKVEVNIEEHIIVHTPPSNLSLQ--LFDAYFNEYMTNYALQELPQNKPLW 121

Query: 62  EIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS-PLTFPSRKFSEKSS 121
           EIH+I  P++N AG +VFK HHSLGDGYSLM +L +   R  DPS PLTFPSRK   + S
Sbjct: 122 EIHIINCPTNNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQS 181

Query: 122 GHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSL 181
           GHIL R+ + L S+MN+V DFG +++KSSVLED LTPIRSGGD GV+FR  AI +I+FSL
Sbjct: 182 GHILGRVYQFLLSSMNTVLDFGGNMMKSSVLEDHLTPIRSGGD-GVEFRTFAIRTITFSL 241

Query: 182 HQLKQIKSKL 190
           H +K+IK+KL
Sbjct: 242 HHIKKIKAKL 248

[Close]

BLAST of MC04g0131 vs. NCBI nr
Match: XP_022969083.1 (O-acyltransferase WSD1-like [Cucurbita maxima])

HSP 1 Score: 239 bits (611), Expect = 8.10e-74
Identity = 125/190 (65.79%), Postives = 149/190 (78.42%), Query Frame = 0


Query: 2   VKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLW 61
           V D NGERKWKKVE+  EEH+ V T PSN SL+  LFDAYF+EYMT+ ALQ+L QN+PLW
Sbjct: 62  VNDVNGERKWKKVELKIEEHIIVHTPPSNLSLQ--LFDAYFNEYMTNYALQELSQNKPLW 121

Query: 62  EIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS-PLTFPSRKFSEKSS 121
           EIH+I  P+SN AG +VFK HHSLGDGYSLM +L +   R  DPS PLTFPSRK   + S
Sbjct: 122 EIHIINCPTSNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQS 181

Query: 122 GHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSL 181
           GHIL R+ + L S+MN+V DFG +I+KSSVLED LTPIRSGGD GV+FR  AI + +FSL
Sbjct: 182 GHILGRVYQFLFSSMNTVLDFGGNIMKSSVLEDHLTPIRSGGD-GVEFRTFAIRTFTFSL 241

Query: 182 HQLKQIKSKL 190
           HQ+KQIK+KL
Sbjct: 242 HQIKQIKAKL 248

[Close]

BLAST of MC04g0131 vs. NCBI nr
Match: XP_023554469.1 (O-acyltransferase WSD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 238 bits (606), Expect = 4.55e-73
Identity = 124/190 (65.26%), Postives = 149/190 (78.42%), Query Frame = 0


Query: 2   VKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLW 61
           V DTNGERKWKKVEV+ EEH+ V T PSN SL+  LFDAYF+EYMT+ ALQ+L QN+PLW
Sbjct: 62  VNDTNGERKWKKVEVNIEEHIIVHTPPSNLSLQ--LFDAYFNEYMTNYALQELSQNKPLW 121

Query: 62  EIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS-PLTFPSRKFSEKSS 121
           EIH+I  P+SN  G +VFK HHSLGDGYSLM +L +   R  DPS PLTFPSRK   + S
Sbjct: 122 EIHIINCPTSNAVGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQS 181

Query: 122 GHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSL 181
           GHIL R+ + L S+MN++ DFGW+I+KSSVLED LTPIRSG D  V+FR  AI +I+FSL
Sbjct: 182 GHILGRVYQFLLSSMNTMLDFGWNIMKSSVLEDHLTPIRSGRDD-VEFRTFAIRTITFSL 241

Query: 182 HQLKQIKSKL 190
            Q+KQIK+KL
Sbjct: 242 PQIKQIKTKL 248

[Close]

BLAST of MC04g0131 vs. ExPASy TrEMBL
Match: A0A0A0K749 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051310 PE=4 SV=1)

HSP 1 Score: 246 bits (628), Expect = 3.18e-79
Identity = 127/197 (64.47%), Postives = 157/197 (79.70%), Query Frame = 0


Query: 2   VKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFEL-FDAYFDEYMTDLALQQLPQNRPL 61
           V D NGERKWKKVEV+ EEH+ VPTFPSN S+  ++ +DAYF++Y+T+L  Q+L +N+PL
Sbjct: 66  VDDINGERKWKKVEVNLEEHILVPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPL 125

Query: 62  WEIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS-PLTFPSRKFSE-- 121
           WEIH+I+YP+SN A  ++ KFHHSLGDGYS+M LL +  KR D+PS PLTFPSRK +   
Sbjct: 126 WEIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDD 185

Query: 122 ---KSSGHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIW 181
              K+  H+LN     + S MNSV DFGWSIIKSSVLEDDLTPIRSGGD GV+FRP+AI 
Sbjct: 186 HNNKNLRHVLN----YVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGGD-GVEFRPVAIC 245

Query: 182 SISFSLHQLKQIKSKLK 191
           +ISFSLHQ+KQIKSKL+
Sbjct: 246 TISFSLHQIKQIKSKLR 257

[Close]

BLAST of MC04g0131 vs. ExPASy TrEMBL
Match: A0A6J1GLS6 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455511 PE=4 SV=1)

HSP 1 Score: 240 bits (613), Expect = 1.97e-74
Identity = 124/190 (65.26%), Postives = 151/190 (79.47%), Query Frame = 0


Query: 2   VKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLW 61
           V D NGERKWKKVEV+ EEH+ V T PSN SL+  LFDAYF+EYMT+ ALQ+LPQN+PLW
Sbjct: 62  VNDVNGERKWKKVEVNIEEHIIVHTPPSNLSLQ--LFDAYFNEYMTNYALQELPQNKPLW 121

Query: 62  EIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS-PLTFPSRKFSEKSS 121
           EIH+I  P++N AG +VFK HHSLGDGYSLM +L +   R  DPS PLTFPSRK   + S
Sbjct: 122 EIHIINCPTNNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQS 181

Query: 122 GHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSL 181
           GHIL R+ + L S+MN+V DFG +++KSSVLED LTPIRSGGD GV+FR  AI +I+FSL
Sbjct: 182 GHILGRVYQFLLSSMNTVLDFGGNMMKSSVLEDHLTPIRSGGD-GVEFRTFAIRTITFSL 241

Query: 182 HQLKQIKSKL 190
           H +K+IK+KL
Sbjct: 242 HHIKKIKAKL 248

[Close]

BLAST of MC04g0131 vs. ExPASy TrEMBL
Match: A0A6J1HZY6 (Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468185 PE=4 SV=1)

HSP 1 Score: 239 bits (611), Expect = 3.92e-74
Identity = 125/190 (65.79%), Postives = 149/190 (78.42%), Query Frame = 0


Query: 2   VKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLW 61
           V D NGERKWKKVE+  EEH+ V T PSN SL+  LFDAYF+EYMT+ ALQ+L QN+PLW
Sbjct: 62  VNDVNGERKWKKVELKIEEHIIVHTPPSNLSLQ--LFDAYFNEYMTNYALQELSQNKPLW 121

Query: 62  EIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS-PLTFPSRKFSEKSS 121
           EIH+I  P+SN AG +VFK HHSLGDGYSLM +L +   R  DPS PLTFPSRK   + S
Sbjct: 122 EIHIINCPTSNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQS 181

Query: 122 GHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSL 181
           GHIL R+ + L S+MN+V DFG +I+KSSVLED LTPIRSGGD GV+FR  AI + +FSL
Sbjct: 182 GHILGRVYQFLFSSMNTVLDFGGNIMKSSVLEDHLTPIRSGGD-GVEFRTFAIRTFTFSL 241

Query: 182 HQLKQIKSKL 190
           HQ+KQIK+KL
Sbjct: 242 HQIKQIKAKL 248

[Close]

BLAST of MC04g0131 vs. ExPASy TrEMBL
Match: A0A6J1GNF5 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455510 PE=4 SV=1)

HSP 1 Score: 230 bits (587), Expect = 3.37e-73
Identity = 119/190 (62.63%), Postives = 148/190 (77.89%), Query Frame = 0


Query: 2   VKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLW 61
           VKD NGERKWKKVE++ EEH+ V T PSN SL+  LFDAYF+EYMT+ ALQ+L QN+PLW
Sbjct: 62  VKDVNGERKWKKVELNIEEHIIVHTPPSNLSLQ--LFDAYFNEYMTNYALQELSQNKPLW 121

Query: 62  EIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS-PLTFPSRKFSEKSS 121
           EIH+I  P+SN  G ++FK HHSLGDGYSLM +L +   R  +PS PLTFP+RK   + S
Sbjct: 122 EIHIINCPTSNAVGNLIFKIHHSLGDGYSLMGVLISTMTRARNPSLPLTFPARKVKLEQS 181

Query: 122 GHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSL 181
           GHIL  +S+ L S+MN+V DFG +++KSSVLEDD TPIRSG D  V+FR   I +I+FSL
Sbjct: 182 GHILGCVSQFLLSSMNTVLDFGGNMMKSSVLEDDFTPIRSGRDD-VEFRTFVIRTITFSL 241

Query: 182 HQLKQIKSKL 190
           HQ+KQIK+KL
Sbjct: 242 HQIKQIKTKL 248

[Close]

BLAST of MC04g0131 vs. ExPASy TrEMBL
Match: A0A6J1GJA7 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454845 PE=4 SV=1)

HSP 1 Score: 235 bits (599), Expect = 2.46e-72
Identity = 123/190 (64.74%), Postives = 148/190 (77.89%), Query Frame = 0


Query: 2   VKDTNGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLW 61
           V D NGERKWK+VEV+ EEH+ V T PSN SL+  LFDAYF+EYMT+ ALQ+LPQN+PLW
Sbjct: 62  VNDVNGERKWKEVEVNIEEHIIVHTPPSNLSLQ--LFDAYFNEYMTNYALQELPQNKPLW 121

Query: 62  EIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS-PLTFPSRKFSEKSS 121
           EIH+I  P+SN AG +VFK  HSLGDGYS M LL +   R  +PS PLTFPSR    + S
Sbjct: 122 EIHIINCPTSNAAGNLVFKIRHSLGDGYSFMGLLISGMTRAHNPSLPLTFPSRTIKAEQS 181

Query: 122 GHILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSL 181
           GHIL R+S+ L S+MN+V DFGW+I+KSSVLED LTPIRSG D  V+FR  AI +I+FSL
Sbjct: 182 GHILGRVSQFLLSSMNTVLDFGWNIMKSSVLEDHLTPIRSGRDD-VEFRTFAIRTITFSL 241

Query: 182 HQLKQIKSKL 190
            Q+KQIK+KL
Sbjct: 242 PQIKQIKTKL 248

[Close]

BLAST of MC04g0131 vs. TAIR 10
Match: AT2G38995.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.4e-19
Identity = 57/189 (30.16%), Postives = 94/189 (49.74%), Query Frame = 0


Query: 6   NGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLWEIHV 65
           NG+  W  V V  E+HV VP    +     E  D + ++Y + LA   +  +RPLWE+H+
Sbjct: 77  NGKASWVPVSVRVEDHVVVPDLDYS---NIENPDQFIEDYTSKLANTPMDMSRPLWELHL 136

Query: 66  IQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS--PLTFPSRKF--SEKSSG 125
           +   +SN   + + KFHHSLGDG SL+SLL    ++T DP   P T  +RK   S K S 
Sbjct: 137 LNIKTSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASSNKKSW 196

Query: 126 HILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSLH 185
            ++ R   ++     +V +    ++    + D  TP+       ++ R +    +SF   
Sbjct: 197 WLVGRFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPLMGKTGDAIRSRKVIHRIVSFD-- 256

Query: 186 QLKQIKSKL 191
            +K +K+ +
Sbjct: 257 DVKLVKNNM 260

[Close]

BLAST of MC04g0131 vs. TAIR 10
Match: AT2G38995.2 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.4e-19
Identity = 57/189 (30.16%), Postives = 94/189 (49.74%), Query Frame = 0


Query: 6   NGERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLWEIHV 65
           NG+  W  V V  E+HV VP    +     E  D + ++Y + LA   +  +RPLWE+H+
Sbjct: 77  NGKASWVPVSVRVEDHVVVPDLDYS---NIENPDQFIEDYTSKLANTPMDMSRPLWELHL 136

Query: 66  IQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPS--PLTFPSRKF--SEKSSG 125
           +   +SN   + + KFHHSLGDG SL+SLL    ++T DP   P T  +RK   S K S 
Sbjct: 137 LNIKTSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASSNKKSW 196

Query: 126 HILNRMSRILSSAMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGVQFRPIAIWSISFSLH 185
            ++ R   ++     +V +    ++    + D  TP+       ++ R +    +SF   
Sbjct: 197 WLVGRFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPLMGKTGDAIRSRKVIHRIVSFD-- 256

Query: 186 QLKQIKSKL 191
            +K +K+ +
Sbjct: 257 DVKLVKNNM 260

[Close]

BLAST of MC04g0131 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 89.0 bits (219), Expect = 4.6e-18
Identity = 62/199 (31.16%), Postives = 106/199 (53.27%), Query Frame = 0


Query: 8   ERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLWEIHVIQ 67
           E+KW +  V  E+HV +P   +   +E    D + + Y++DL    L  ++PLWE+H++ 
Sbjct: 87  EQKWVRTNVVVEDHVIIPKIQTQ-HIENANADVFLESYVSDLTTIPLDTSKPLWEVHLLD 146

Query: 68  YPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPSPL-TFP------SRKFSEKSS 127
             +S+   + V + HHSLGDG S+MSL+  C ++T +P+ L + P      S   S K+S
Sbjct: 147 LKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSGSSSLKTS 206

Query: 128 GHILNR---MSRILSSA----MNSVTDFGWSIIKSSVLEDDLTPIRSGGDHGV-QFRPIA 187
               +R   +  +L SA    +N+V D    I  +  L+D  TPI+  GD  + + + + 
Sbjct: 207 SRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIK--GDFKLSKGKRMC 266

Query: 188 IWSISFSLHQLKQIKSKLK 192
           +   + SL  +K IK+ +K
Sbjct: 267 MVHRTVSLDDIKLIKNAMK 282

[Close]

BLAST of MC04g0131 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 89.0 bits (219), Expect = 4.6e-18
Identity = 59/205 (28.78%), Postives = 101/205 (49.27%), Query Frame = 0


Query: 2   VKDTNGE-RKWKKVEVDPEEHVFVPTF-PSNWSLEFELFDAYFDEYMTDLALQQLPQNRP 61
           V D NG+ ++W +  V  E+H  VP   P N     E  +A+ ++Y++DL    L  +RP
Sbjct: 70  VTDENGQNQRWVRTNVVVEDHFIVPKIKPQN----IENSNAFLEDYVSDLMKIPLDTSRP 129

Query: 62  LWEIHVIQYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPSPLTFPSRKFSEKS 121
           LWE+H++   +S+   + V K HHS+GDG S+MSL+  C ++T +P  L     ++   S
Sbjct: 130 LWELHLLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSS 189

Query: 122 SGHILNRMSRILSS--------------AMNSVTDFGWSIIKSSVLEDDLTPIRSGGDHG 181
              +L   SR  S                +N+V D    I+ +  ++D  TPI+ G    
Sbjct: 190 RSSLLTTGSRSDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIK-GDFLS 249

Query: 182 VQFRPIAIWSISFSLHQLKQIKSKL 191
            + + + +   + SL  +K  K+ +
Sbjct: 250 TKSKQLRLVHRTVSLDDIKLTKNAM 269

[Close]

BLAST of MC04g0131 vs. TAIR 10
Match: AT1G72110.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 88.2 bits (217), Expect = 7.8e-18
Identity = 41/106 (38.68%), Postives = 65/106 (61.32%), Query Frame = 0


Query: 7   GERKWKKVEVDPEEHVFVPTFPSNWSLEFELFDAYFDEYMTDLALQQLPQNRPLWEIHVI 66
           G+ +W   +++ EEHV VP    N     E  D + ++Y +++AL  +  ++PLWE H++
Sbjct: 72  GKARWIPTKINVEEHVIVPDIDPN----IENPDEFLEDYTSNMALSPMDMSKPLWEFHLL 131

Query: 67  QYPSSNVAGIIVFKFHHSLGDGYSLMSLLFTCFKRTDDPSPLTFPS 113
           +  +S+   + V +FHHSLGDG SLMSLL  C ++T DP    FP+
Sbjct: 132 KLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPE--AFPT 171

The following BLAST results are available for this feature:

BLAST of MC04g0131 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IU14	3.4e-18	30.16	Wax ester synthase/diacylglycerol acyltransferase 3 OS=Arabidopsis thaliana OX=3...	[more]
Q9M3B3	6.4e-17	28.78	Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3...	[more]
Q9M3B2	6.4e-17	31.16	Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3...	[more]
Q9C7H4	1.1e-16	38.68	Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3...	[more]
Q9FK04	2.4e-16	31.47	Wax ester synthase/diacylglycerol acyltransferase 10 OS=Arabidopsis thaliana OX=...	[more]

BLAST of MC04g0131 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888791.1	3.77e-79	64.43	O-acyltransferase WSD1-like [Benincasa hispida]	[more]
KAE8645785.1	6.57e-79	64.47	hypothetical protein Csa_017199 [Cucumis sativus]	[more]
XP_022952996.1	4.06e-74	65.26	O-acyltransferase WSD1-like [Cucurbita moschata]	[more]
XP_022969083.1	8.10e-74	65.79	O-acyltransferase WSD1-like [Cucurbita maxima]	[more]
XP_023554469.1	4.55e-73	65.26	O-acyltransferase WSD1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC04g0131 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K749	3.18e-79	64.47	Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051310 PE=4...	[more]
A0A6J1GLS6	1.97e-74	65.26	Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455511 P...	[more]
A0A6J1HZY6	3.92e-74	65.79	Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468185 PE=...	[more]
A0A6J1GNF5	3.37e-73	62.63	Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455510 P...	[more]
A0A6J1GJA7	2.46e-72	64.74	Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454845 P...	[more]

BLAST of MC04g0131 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38995.1	2.4e-19	30.16	O-acyltransferase (WSD1-like) family protein	[more]
AT2G38995.2	2.4e-19	30.16	O-acyltransferase (WSD1-like) family protein	[more]
AT3G49200.1	4.6e-18	31.16	O-acyltransferase (WSD1-like) family protein	[more]
AT3G49190.1	4.6e-18	28.78	O-acyltransferase (WSD1-like) family protein	[more]
AT1G72110.1	7.8e-18	38.68	O-acyltransferase (WSD1-like) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004255	O-acyltransferase, WSD1, N-terminal	PFAM	PF03007	WES_acyltransf	coord: 42..138 e-value: 1.5E-9 score: 38.0
None	No IPR available	PANTHER	PTHR31650:SF34	O-ACYLTRANSFERASE WSD1-LIKE	coord: 2..190
IPR045034	O-acyltransferase WSD1-like	PANTHER	PTHR31650	O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN	coord: 2..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0131.1	MC04g0131.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019432	triglyceride biosynthetic process
biological_process	GO:0045017	glycerolipid biosynthetic process
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0004144	diacylglycerol O-acyltransferase activity
molecular_function	GO:0047196	long-chain-alcohol O-fatty-acyltransferase activity
molecular_function	GO:0008374	O-acyltransferase activity