MC04g0085 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0085
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	1-aminocyclopropane-1-carboxylate oxidase 1
Location	MC04: 624213 .. 626135 (-)
RNA-Seq Expression	MC04g0085
Synteny	MC04g0085

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGGCATTTTTATTTGATGGAATAAATGGAAGATGCAAGGAATGAGGGCATGGGAGGCATTGGCATTGGGAGGGCAGCAGCCATACCCTCCATTTTTGGTCTAACAAAAGAGATGAAAAAGCCATCAAAGTGGGCCCTTCCCCACCAAAGAGGAATTTAGTACACTCTTCGACCAGCTAAAGCCAAGACCAAATGGGCCCCATGCCATTATTTATCACTTTTTTCATAAATATATAAAAATAAATAAATAAAATAAAATAAAAATCATCAATTTCTTCCCTCCTTCCACTCCTTAAATCCAAAACTCTTCCCTATAAATACCCCTCCATTATACTTGATCCAACACACCCACCCACCAAAAAACCAAAGCAAAGTACTTTCTTTCCCCATATCTCCAAAAAAAAAAAATGGCCATTTTTCCAGTCATCAACTTGGACAGCATCAATGGCGATGCTAGGCCCAAGATTTTGGAGCAAATTGAAGACGCATGTCGAAACTGGGGTTTTTTTGAGGTGAAGATTCGAACTTACAACTTTTAGAACAAATATTAGAGATGTTTGATGTCGAAGCCGCGTAATTGATTGTGATGGGAGGTTTAATTTGGGTTCGTGGGTTTGATTTGATTGCAGGTGGTGAACCATGGGATCCCACATGAGTTTTTGGACAGAGTGGAGAAGATGACGAGAGATCACTACAAGAAATGTATGGAAGAGAGGTTCAAGGAGACGGTGGCAAGCAAGGGCTTAGAGGCTGCACAGGCTGAAGTTAATGATATGGATTGGGAGAGTACCTTTTTCTTGCGCCATCTTCCTCAATCAAACATCTCCGAGATCCCCGATCTCGACGACGAGTACAGGTAAGACAACGAACCTCTTCATATTTCTACGATGATATTATCTATTTTAGACATGAGGTGTCGCGTGTCACTCTTTACTAATGTCTTGTGCATGATATGTGCAGGAAAGTTATGAAGGAATTTGCCAAGTTGTTGGAGAAACTTGCTGAGGAGCTATTGGACATGCTATGCGAGAATCTTGGGTTGGAGAAAGGCTATCTGAAGAAGGCTTTCTATGGATCACAAGGTCCAACATTTGGCACAAAGGTGAGCAATTACCCACCGTGTCCGAAGCCGGAGCTGATCAAAGGTCTCCGAGCCCACACCGACGCCGGTGGCATCATCCTCCTCTTCCAAGACGACAAGGTCAGTGGCCTACAGCTCCTCAAAGATGGCAACTGGATCGACGTGCCCCCAATGCGCCATGCCATCGTAGTCAACCTTGGGGATCAGCTTGAGGTAAGTACATCATCAAAACCAAATCAACCCAGTAGAGGAGTACTCTTAACTTCCTAAATTTTCAAAATGGAAAGATTATATTATACAAAAAAGTTGAAACGTAAAACTTTGCAGGTGATCACAAACGGAAGATACAAAAGTGTGATGCATAGAGTCATAACTCAAGCGAACGGAACCGGCCGAATGTCGATAGCTTCGTTCTACAATCCCGGGAGCGACGCCGTGATCTTCCCGGCGCCAGCGCTGGTGGAGAAAGAGGCTGAGGAGAAGAATGGAGTGTATCCCAAGTTTGTGTTTGAAGATTACATGAAGCTGTACTTAGGCGTCAAGTTTGAGGCCAAGGAGCCAAGATTTGAAGCCATGAAAGCCAATGCCAATTTGGGTCCAATGGCAACAGCATAAAATAGAGAAACCAAAATAAAGACACCCACTTTTTTTAACAAATAAATATATTGAAAGGTTTGGTTGCCTTTTTAAGTGGGTTGTGAGTGACTGAACTATAGGTTAATGTTATTAATTTAATGATAAGTTAAAAAGCAAGTTTGCAGAAGTAGAATATGTTTGTAGCAAAAGTGATGTGTTTCCATTTTCTCTATATAATTAAATTCACTTTCAGTTTCAAGGGTCCA

mRNA sequence

GTGGCATTTTTATTTGATGGAATAAATGGAAGATGCAAGGAATGAGGGCATGGGAGGCATTGGCATTGGGAGGGCAGCAGCCATACCCTCCATTTTTGGTCTAACAAAAGAGATGAAAAAGCCATCAAAGTGGGCCCTTCCCCACCAAAGAGGAATTTAGTACACTCTTCGACCAGCTAAAGCCAAGACCAAATGGGCCCCATGCCATTATTTATCACTTTTTTCATAAATATATAAAAATAAATAAATAAAATAAAATAAAAATCATCAATTTCTTCCCTCCTTCCACTCCTTAAATCCAAAACTCTTCCCTATAAATACCCCTCCATTATACTTGATCCAACACACCCACCCACCAAAAAACCAAAGCAAAGTACTTTCTTTCCCCATATCTCCAAAAAAAAAAAATGGCCATTTTTCCAGTCATCAACTTGGACAGCATCAATGGCGATGCTAGGCCCAAGATTTTGGAGCAAATTGAAGACGCATGTCGAAACTGGGGTTTTTTTGAGGTGGTGAACCATGGGATCCCACATGAGTTTTTGGACAGAGTGGAGAAGATGACGAGAGATCACTACAAGAAATGTATGGAAGAGAGGTTCAAGGAGACGGTGGCAAGCAAGGGCTTAGAGGCTGCACAGGCTGAAGTTAATGATATGGATTGGGAGAGTACCTTTTTCTTGCGCCATCTTCCTCAATCAAACATCTCCGAGATCCCCGATCTCGACGACGAGTACAGGAAAGTTATGAAGGAATTTGCCAAGTTGTTGGAGAAACTTGCTGAGGAGCTATTGGACATGCTATGCGAGAATCTTGGGTTGGAGAAAGGCTATCTGAAGAAGGCTTTCTATGGATCACAAGGTCCAACATTTGGCACAAAGGTGAGCAATTACCCACCGTGTCCGAAGCCGGAGCTGATCAAAGGTCTCCGAGCCCACACCGACGCCGGTGGCATCATCCTCCTCTTCCAAGACGACAAGGTCAGTGGCCTACAGCTCCTCAAAGATGGCAACTGGATCGACGTGCCCCCAATGCGCCATGCCATCGTAGTCAACCTTGGGGATCAGCTTGAGGTGATCACAAACGGAAGATACAAAAGTGTGATGCATAGAGTCATAACTCAAGCGAACGGAACCGGCCGAATGTCGATAGCTTCGTTCTACAATCCCGGGAGCGACGCCGTGATCTTCCCGGCGCCAGCGCTGGTGGAGAAAGAGGCTGAGGAGAAGAATGGAGTGTATCCCAAGTTTGTGTTTGAAGATTACATGAAGCTGTACTTAGGCGTCAAGTTTGAGGCCAAGGAGCCAAGATTTGAAGCCATGAAAGCCAATGCCAATTTGGGTCCAATGGCAACAGCATAAAATAGAGAAACCAAAATAAAGACACCCACTTTTTTTAACAAATAAATATATTGAAAGGTTTGGTTGCCTTTTTAAGTGGGTTGTGAGTGACTGAACTATAGGTTAATGTTATTAATTTAATGATAAGTTAAAAAGCAAGTTTGCAGAAGTAGAATATGTTTGTAGCAAAAGTGATGTGTTTCCATTTTCTCTATATAATTAAATTCACTTTCAGTTTCAAGGGTCCA

Coding sequence (CDS)

ATGGCCATTTTTCCAGTCATCAACTTGGACAGCATCAATGGCGATGCTAGGCCCAAGATTTTGGAGCAAATTGAAGACGCATGTCGAAACTGGGGTTTTTTTGAGGTGGTGAACCATGGGATCCCACATGAGTTTTTGGACAGAGTGGAGAAGATGACGAGAGATCACTACAAGAAATGTATGGAAGAGAGGTTCAAGGAGACGGTGGCAAGCAAGGGCTTAGAGGCTGCACAGGCTGAAGTTAATGATATGGATTGGGAGAGTACCTTTTTCTTGCGCCATCTTCCTCAATCAAACATCTCCGAGATCCCCGATCTCGACGACGAGTACAGGAAAGTTATGAAGGAATTTGCCAAGTTGTTGGAGAAACTTGCTGAGGAGCTATTGGACATGCTATGCGAGAATCTTGGGTTGGAGAAAGGCTATCTGAAGAAGGCTTTCTATGGATCACAAGGTCCAACATTTGGCACAAAGGTGAGCAATTACCCACCGTGTCCGAAGCCGGAGCTGATCAAAGGTCTCCGAGCCCACACCGACGCCGGTGGCATCATCCTCCTCTTCCAAGACGACAAGGTCAGTGGCCTACAGCTCCTCAAAGATGGCAACTGGATCGACGTGCCCCCAATGCGCCATGCCATCGTAGTCAACCTTGGGGATCAGCTTGAGGTGATCACAAACGGAAGATACAAAAGTGTGATGCATAGAGTCATAACTCAAGCGAACGGAACCGGCCGAATGTCGATAGCTTCGTTCTACAATCCCGGGAGCGACGCCGTGATCTTCCCGGCGCCAGCGCTGGTGGAGAAAGAGGCTGAGGAGAAGAATGGAGTGTATCCCAAGTTTGTGTTTGAAGATTACATGAAGCTGTACTTAGGCGTCAAGTTTGAGGCCAAGGAGCCAAGATTTGAAGCCATGAAAGCCAATGCCAATTTGGGTCCAATGGCAACAGCATAA

Protein sequence

MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKCMEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKLLEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDAGGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQANGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEPRFEAMKANANLGPMATA

Homology

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BLAST of MC04g0085 vs. ExPASy Swiss-Prot
Match: Q04644 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 1.6e-169
Identity = 285/318 (89.62%), Postives = 305/318 (95.91%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MA+FP+INL++IN D R KILEQIEDAC+NWGFFE+VNHGIPHEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           MEERFKETV SKGLEAAQAEVNDMDWESTFFLRHLP+SNIS++ DLD+EY+K+MKEFAK 
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LE LAEELLD+LCENLGLEKGYLKKAFYGS+GPTFGTKVSNYPPCPKP+LIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQ 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEA-EEKNGVYPKFVFEDYMKLYLGVKFEAKE 300
           +GTGRMSIASFYNPGSDAVI+PAPALVEK+  EEK  VYPKFVFEDYMKLYLGVKF+AKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPMATA 318
           PRFEAMKANANLGPMATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

[Close]

BLAST of MC04g0085 vs. ExPASy Swiss-Prot
Match: Q9MB94 (1-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume OX=102107 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 3.7e-150
Identity = 251/320 (78.44%), Postives = 288/320 (90.00%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           M  FP+INL+ +NG+ R   +E+I+DAC NWGFFE+V+HGIP EFLD VE++T++HY++C
Sbjct: 1   MENFPIINLEGLNGEGRKATMEKIKDACENWGFFELVSHGIPTEFLDTVERLTKEHYRQC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           +E+RFKE VASKGLEA + EVNDMDWESTF+LRHLP+SNISE+PDL+D+YR VMKEFA  
Sbjct: 61  LEQRFKELVASKGLEAVKTEVNDMDWESTFYLRHLPKSNISEVPDLEDQYRNVMKEFALK 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LEKLAE+LLD+LCENLGLEKGYLKKAFYG+ GPTFGTKVSNYPPCP PELIKGLRAHTDA
Sbjct: 121 LEKLAEQLLDLLCENLGLEKGYLKKAFYGTNGPTFGTKVSNYPPCPNPELIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GG+ILLFQDDKVSGLQLLKDG WIDVPPMRH+IV+NLGDQLEVITNG+Y+SV HRVI Q 
Sbjct: 181 GGLILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVINLGDQLEVITNGKYRSVEHRVIAQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300
           +GT RMSIASFYNPGSDAVI+PAP LVEKEAEEKN VYPKFVFEDYMKLY G+KF+ KEP
Sbjct: 241 DGT-RMSIASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYAGLKFQPKEP 300

Query: 301 RFEAMKA---NANLGPMATA 318
           RFEAMKA   N +L P+ATA
Sbjct: 301 RFEAMKAVETNISLVPIATA 319

[Close]

BLAST of MC04g0085 vs. ExPASy Swiss-Prot
Match: Q08507 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 8.1e-150
Identity = 254/321 (79.13%), Postives = 286/321 (89.10%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           M  FP+INL+ +NG  R   +E I+DAC NWGFFE+VNHGIPHE +D VEK+T+ HYKKC
Sbjct: 1   MENFPIINLEKLNGSERDATMEMIKDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           ME+RFKE VASKGLEA QAEV D+DWESTFFLRHLP SNISE+PDLDDEYR+VM++FAK 
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLDDEYREVMRDFAKR 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LEKLAEELLD+LCENLGLEKGYLKKAFYGS+GP FGTKVSNYPPCPKP+LIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRH+IVVNLGDQLEVITNG+YKSV+HRVI Q 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEK-NGVYPKFVFEDYMKLYLGVKFEAKE 300
           +GT RMS+ASFYNPGSDAVI+PAP LVEKEA+++   VYPKFVF+DYMKLY G+KF+AKE
Sbjct: 241 DGT-RMSLASFYNPGSDAVIYPAPTLVEKEADQECKQVYPKFVFDDYMKLYAGLKFQAKE 300

Query: 301 PRFEAMK---ANANLGPMATA 318
           PRFEAMK   A+    P+ATA
Sbjct: 301 PRFEAMKAREADVKSDPIATA 320

[Close]

BLAST of MC04g0085 vs. ExPASy Swiss-Prot
Match: P05116 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=ACO1 PE=2 SV=2)

HSP 1 Score: 530.0 bits (1364), Expect = 1.8e-149
Identity = 252/317 (79.50%), Postives = 283/317 (89.27%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           M  FP+INL+ +NGD R   +E I+DAC NWGFFE+VNHGIPHE +D VEKMT+ HYKKC
Sbjct: 1   MENFPIINLEKLNGDERANTMEMIKDACENWGFFELVNHGIPHEVMDTVEKMTKGHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           ME+RFKE VASKGLEA QAEV D+DWESTFFLRHLP SNIS++PDLD+EYR+VM++FAK 
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPTSNISQVPDLDEEYREVMRDFAKR 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LEKLAEELLD+LCENLGLEKGYLK AFYGS+GP FGTKVSNYPPCPKP+LIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKD  WIDVPPMRH+IVVNLGDQLEVITNG+YKSV+HRVI Q 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDEQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300
           +GT RMS+ASFYNPGSDAVI+PA  LVEKEAEE   VYPKFVF+DYMKLY G+KF+AKEP
Sbjct: 241 DGT-RMSLASFYNPGSDAVIYPAKTLVEKEAEESTQVYPKFVFDDYMKLYAGLKFQAKEP 300

Query: 301 RFEAMKANANLGPMATA 318
           RFEAMKA  +  P+A+A
Sbjct: 301 RFEAMKAMES-DPIASA 315

[Close]

BLAST of MC04g0085 vs. ExPASy Swiss-Prot
Match: Q08506 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 9.0e-149
Identity = 252/319 (79.00%), Postives = 281/319 (88.09%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           M  FP+I+LD +NG  R   +E I+DAC NWGFFE+VNHGIP E +D VEKMT+ HYKKC
Sbjct: 1   MENFPIISLDKVNGVERAATMEMIKDACENWGFFELVNHGIPREVMDTVEKMTKGHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           ME+RFKE VASK LE  QAEV DMDWESTFFL+HLP SNISE+PDLD+EYR+VM++FAK 
Sbjct: 61  MEQRFKELVASKALEGVQAEVTDMDWESTFFLKHLPISNISEVPDLDEEYREVMRDFAKR 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LEKLAEELLD+LCENLGLEKGYLK AFYGS+GP FGTKVSNYPPCPKP+LIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRH+IVVNLGDQLEVITNG+YKSVMHRVI Q 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVMHRVIAQK 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300
           +G  RMS+ASFYNPGSDAVI+PAPALVEKEAEE   VYPKFVF+DYMKLY G+KF+AKEP
Sbjct: 241 DG-ARMSLASFYNPGSDAVIYPAPALVEKEAEENKQVYPKFVFDDYMKLYAGLKFQAKEP 300

Query: 301 RFEAMKA---NANLGPMAT 317
           RFEAMKA   +  + P+AT
Sbjct: 301 RFEAMKAMETDVKMDPIAT 318

[Close]

BLAST of MC04g0085 vs. NCBI nr
Match: XP_022148024.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Momordica charantia])

HSP 1 Score: 647 bits (1669), Expect = 2.05e-234
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC
Sbjct: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL
Sbjct: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA
Sbjct: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300
           NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP
Sbjct: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300

Query: 301 RFEAMKANANLGPMATA 317
           RFEAMKANANLGPMATA
Sbjct: 301 RFEAMKANANLGPMATA 317

[Close]

BLAST of MC04g0085 vs. NCBI nr
Match: AFF19571.1 (1-aminocyclopropane-1-carboxylate oxidase [Momordica charantia])

HSP 1 Score: 642 bits (1657), Expect = 1.38e-232
Identity = 315/317 (99.37%), Postives = 316/317 (99.68%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC
Sbjct: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL
Sbjct: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LEKLAEELLDMLCENLGLE+GYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRA TDA
Sbjct: 121 LEKLAEELLDMLCENLGLEEGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRARTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300
           NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP
Sbjct: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300

Query: 301 RFEAMKANANLGPMATA 317
           RFEAMKANANLGPMATA
Sbjct: 301 RFEAMKANANLGPMATA 317

[Close]

BLAST of MC04g0085 vs. NCBI nr
Match: AEM42994.1 (ACC oxidase [Siraitia grosvenorii])

HSP 1 Score: 602 bits (1551), Expect = 1.99e-216
Identity = 289/317 (91.17%), Postives = 306/317 (96.53%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MA FPVINL++INGD R KI+EQ+EDAC+NWGFFE+VNHGIPHEFLD VEKMTR HYKKC
Sbjct: 1   MANFPVINLENINGDGRAKIMEQLEDACQNWGFFELVNHGIPHEFLDTVEKMTRYHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           ME+RFKETVASKGLEAAQAEV DMDWESTFFLRHLP+SNISEIPDLDD+YRK+MKEFAK 
Sbjct: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNISEIPDLDDDYRKIMKEFAKK 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LEKLAEELLD+LCENLGLE GYLKKAFYGS+GPTFGTKVSNYPPCPKPELIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLENGYLKKAFYGSKGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDG+WIDVPPMRHAIVVNLGDQLEVITNG+YKSVMHRV+TQA
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGHWIDVPPMRHAIVVNLGDQLEVITNGKYKSVMHRVVTQA 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300
           NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEE N VYPKFVFEDYMKLY+GVKF+AKEP
Sbjct: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEENNNVYPKFVFEDYMKLYVGVKFQAKEP 300

Query: 301 RFEAMKANANLGPMATA 317
           RFEAMKANANLGPMA A
Sbjct: 301 RFEAMKANANLGPMAIA 317

[Close]

BLAST of MC04g0085 vs. NCBI nr
Match: KAA0061654.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa] >TYK05852.1 1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 595 bits (1533), Expect = 1.14e-213
Identity = 286/318 (89.94%), Postives = 306/318 (96.23%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MA+FP+INL++INGD R KILEQIEDAC+NWGFFE+VNHGIPHEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           MEERFKETV SKGLEAAQAEVNDMDWESTFFLRHLP+SNIS++ DLD+EY+K+MKEFAK 
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LE LAEELLD+LCENLGLEKGYLKKAFYGS+GPTFGTKVSNYPPCPKP+LIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQ 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEA-EEKNGVYPKFVFEDYMKLYLGVKFEAKE 300
           +GTGRMSIASFYNPGSDAVI+PAPALVEK+  EEK  VYPKFVFEDYMKLYLGVKF+AKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPMATA 317
           PRFEAMKANANLGPMATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

[Close]

BLAST of MC04g0085 vs. NCBI nr
Match: NP_001284392.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo] >Q04644.1 RecName: Full=1-aminocyclopropane-1-carboxylate oxidase 1; Short=ACC oxidase 1; AltName: Full=Ethylene-forming enzyme; Short=EFE; AltName: Full=PMEL1 [Cucumis melo] >ACY08855.1 ACC oxidase [Cucumis melo] >CAA49553.1 enzyme-forming ethylene [Cucumis melo] >CAA64797.1 ACC oxidase [Cucumis melo] >BAA06526.1 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo])

HSP 1 Score: 592 bits (1526), Expect = 1.33e-212
Identity = 285/318 (89.62%), Postives = 305/318 (95.91%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MA+FP+INL++IN D R KILEQIEDAC+NWGFFE+VNHGIPHEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           MEERFKETV SKGLEAAQAEVNDMDWESTFFLRHLP+SNIS++ DLD+EY+K+MKEFAK 
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LE LAEELLD+LCENLGLEKGYLKKAFYGS+GPTFGTKVSNYPPCPKP+LIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQ 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEA-EEKNGVYPKFVFEDYMKLYLGVKFEAKE 300
           +GTGRMSIASFYNPGSDAVI+PAPALVEK+  EEK  VYPKFVFEDYMKLYLGVKF+AKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPMATA 317
           PRFEAMKANANLGPMATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

[Close]

BLAST of MC04g0085 vs. ExPASy TrEMBL
Match: A0A6J1D2S4 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Momordica charantia OX=3673 GN=LOC111016812 PE=3 SV=1)

HSP 1 Score: 647 bits (1669), Expect = 9.90e-235
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC
Sbjct: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL
Sbjct: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA
Sbjct: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300
           NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP
Sbjct: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300

Query: 301 RFEAMKANANLGPMATA 317
           RFEAMKANANLGPMATA
Sbjct: 301 RFEAMKANANLGPMATA 317

[Close]

BLAST of MC04g0085 vs. ExPASy TrEMBL
Match: H9NAH9 (1-aminocyclopropane-1-carboxylate oxidase OS=Momordica charantia OX=3673 PE=2 SV=1)

HSP 1 Score: 642 bits (1657), Expect = 6.69e-233
Identity = 315/317 (99.37%), Postives = 316/317 (99.68%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC
Sbjct: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL
Sbjct: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LEKLAEELLDMLCENLGLE+GYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRA TDA
Sbjct: 121 LEKLAEELLDMLCENLGLEEGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRARTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300
           NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP
Sbjct: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300

Query: 301 RFEAMKANANLGPMATA 317
           RFEAMKANANLGPMATA
Sbjct: 301 RFEAMKANANLGPMATA 317

[Close]

BLAST of MC04g0085 vs. ExPASy TrEMBL
Match: K7NBW2 (ACC oxidase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 602 bits (1551), Expect = 9.64e-217
Identity = 289/317 (91.17%), Postives = 306/317 (96.53%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MA FPVINL++INGD R KI+EQ+EDAC+NWGFFE+VNHGIPHEFLD VEKMTR HYKKC
Sbjct: 1   MANFPVINLENINGDGRAKIMEQLEDACQNWGFFELVNHGIPHEFLDTVEKMTRYHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           ME+RFKETVASKGLEAAQAEV DMDWESTFFLRHLP+SNISEIPDLDD+YRK+MKEFAK 
Sbjct: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNISEIPDLDDDYRKIMKEFAKK 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LEKLAEELLD+LCENLGLE GYLKKAFYGS+GPTFGTKVSNYPPCPKPELIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLENGYLKKAFYGSKGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDG+WIDVPPMRHAIVVNLGDQLEVITNG+YKSVMHRV+TQA
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGHWIDVPPMRHAIVVNLGDQLEVITNGKYKSVMHRVVTQA 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEP 300
           NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEE N VYPKFVFEDYMKLY+GVKF+AKEP
Sbjct: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEENNNVYPKFVFEDYMKLYVGVKFQAKEP 300

Query: 301 RFEAMKANANLGPMATA 317
           RFEAMKANANLGPMA A
Sbjct: 301 RFEAMKANANLGPMAIA 317

[Close]

BLAST of MC04g0085 vs. ExPASy TrEMBL
Match: A0A5D3C3Q0 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00060 PE=3 SV=1)

HSP 1 Score: 595 bits (1533), Expect = 5.54e-214
Identity = 286/318 (89.94%), Postives = 306/318 (96.23%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MA+FP+INL++INGD R KILEQIEDAC+NWGFFE+VNHGIPHEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           MEERFKETV SKGLEAAQAEVNDMDWESTFFLRHLP+SNIS++ DLD+EY+K+MKEFAK 
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LE LAEELLD+LCENLGLEKGYLKKAFYGS+GPTFGTKVSNYPPCPKP+LIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQ 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEA-EEKNGVYPKFVFEDYMKLYLGVKFEAKE 300
           +GTGRMSIASFYNPGSDAVI+PAPALVEK+  EEK  VYPKFVFEDYMKLYLGVKF+AKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPMATA 317
           PRFEAMKANANLGPMATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

[Close]

BLAST of MC04g0085 vs. ExPASy TrEMBL
Match: D0V4H6 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103491357 PE=2 SV=1)

HSP 1 Score: 592 bits (1526), Expect = 6.46e-213
Identity = 285/318 (89.62%), Postives = 305/318 (95.91%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MA+FP+INL++IN D R KILEQIEDAC+NWGFFE+VNHGIPHEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           MEERFKETV SKGLEAAQAEVNDMDWESTFFLRHLP+SNIS++ DLD+EY+K+MKEFAK 
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           LE LAEELLD+LCENLGLEKGYLKKAFYGS+GPTFGTKVSNYPPCPKP+LIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQ 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEA-EEKNGVYPKFVFEDYMKLYLGVKFEAKE 300
           +GTGRMSIASFYNPGSDAVI+PAPALVEK+  EEK  VYPKFVFEDYMKLYLGVKF+AKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPMATA 317
           PRFEAMKANANLGPMATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

[Close]

BLAST of MC04g0085 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 508.1 bits (1307), Expect = 5.2e-144
Identity = 237/323 (73.37%), Postives = 283/323 (87.62%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           M  FP+INL+ +NG+ R   +E+I+DAC NWGFFE VNHGI  E LD+VEKMT++HYKKC
Sbjct: 1   MESFPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKC 60

Query: 61  MEERFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKL 120
           MEERFKE++ ++GL++ ++EVND+DWESTF+L+HLP SNIS++PDLDD+YR +MK+FA  
Sbjct: 61  MEERFKESIKNRGLDSLRSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDFAGK 120

Query: 121 LEKLAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180
           +EKL+EELLD+LCENLGLEKGYLKK FYGS+ PTFGTKVSNYPPCP P+L+KGLRAHTDA
Sbjct: 121 IEKLSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQA 240
           GGIILLFQDDKVSGLQLLKDG W+DVPP++H+IVVNLGDQLEVITNG+YKSV HRV++Q 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEHRVLSQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAE-EKNGVYPKFVFEDYMKLYLGVKFEAKE 300
           +G GRMSIASFYNPGSD+VIFPAP L+ KEAE EK   YP+FVFEDYMKLY  VKF+AKE
Sbjct: 241 DGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAVKFQAKE 300

Query: 301 PRFEAMKANA-----NLGPMATA 318
           PRFEAMKA       N+GP+ATA
Sbjct: 301 PRFEAMKAMETTVANNVGPLATA 323

[Close]

BLAST of MC04g0085 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 469.2 bits (1206), Expect = 2.7e-132
Identity = 216/315 (68.57%), Postives = 263/315 (83.49%), Query Frame = 0


Query: 4   FPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKCMEE 63
           FPV++L  +NG+ R + +  I +AC NWGFFE+VNHG+PH+ +D++EKMT+DHYK C E+
Sbjct: 7   FPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQEQ 66

Query: 64  RFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKLLEK 123
           +F + + SKGL+  + EV D+DWESTF++RHLPQSN+++I D+ DEYR  MK+F K LE 
Sbjct: 67  KFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAMKDFGKRLEN 126

Query: 124 LAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDAGGI 183
           LAE+LLD+LCENLGLEKGYLKK F+G++GPTFGTKVSNYPPCPKPE+IKGLRAHTDAGGI
Sbjct: 127 LAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHTDAGGI 186

Query: 184 ILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQANGT 243
           ILLFQDDKVSGLQLLKDG+WIDVPP+ H+IV+NLGDQLEVITNG+YKSV+HRV+TQ  G 
Sbjct: 187 ILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYKSVLHRVVTQQEG- 246

Query: 244 GRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEPRFE 303
            RMS+ASFYNPGSDA I PA +LVEK++E     YP FVF+DYMKLY GVKF+ KEPRF 
Sbjct: 247 NRMSVASFYNPGSDAEISPATSLVEKDSE-----YPSFVFDDYMKLYAGVKFQPKEPRFA 306

Query: 304 AMK---ANANLGPMA 316
           AMK   A   L P A
Sbjct: 307 AMKNASAVTELNPTA 315

[Close]

BLAST of MC04g0085 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 468.0 bits (1203), Expect = 6.0e-132
Identity = 217/315 (68.89%), Postives = 269/315 (85.40%), Query Frame = 0


Query: 4   FPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKCMEE 63
           FPVI+L  +NG+ R + +  I+DAC+NWGFFE+VNHG+P++ +D +E+MT++HYKK ME+
Sbjct: 7   FPVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHMEQ 66

Query: 64  RFKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKLLEK 123
           +FKE + SKGL+  + EV D+DWESTF+L HLPQSN+ +IPD+ +EYR  MK+F K LE 
Sbjct: 67  KFKEMLRSKGLDTLETEVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAMKDFGKRLEI 126

Query: 124 LAEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDAGGI 183
           LAEELLD+LCENLGLEKGYLKK F+G+ GPTF TK+SNYPPCPKPE+IKGLRAHTDAGG+
Sbjct: 127 LAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTDAGGL 186

Query: 184 ILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQANGT 243
           ILLFQDDKVSGLQLLKDG+W+DVPP++H+IV+NLGDQLEVITNG+YKSVMHRV+TQ  G 
Sbjct: 187 ILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRVMTQKEG- 246

Query: 244 GRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLYLGVKFEAKEPRFE 303
            RMSIASFYNPGSDA I PA +LV+K+++     YP FVF+DYMKLY G+KF+AKEPRFE
Sbjct: 247 NRMSIASFYNPGSDAEISPATSLVDKDSK-----YPSFVFDDYMKLYAGLKFQAKEPRFE 306

Query: 304 AMK---ANANLGPMA 316
           AMK   A A+L P+A
Sbjct: 307 AMKNAEAAADLNPVA 315

[Close]

BLAST of MC04g0085 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 311.2 bits (796), Expect = 9.4e-85
Identity = 150/308 (48.70%), Postives = 209/308 (67.86%), Query Frame = 0


Query: 5   PVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKCMEER 64
           PVI+   ++G+ R K +  ++ AC  WGFF V NHGI  E +++V+KM   HY++ ++E+
Sbjct: 12  PVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHLKEK 71

Query: 65  FKETVASKGLEAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMKEFAKLLEKL 124
           F ++   K L  ++ + +D DWES+FF+ H P SNI +IP++ +E  K M E+   L K 
Sbjct: 72  FYQSEMVKAL--SEGKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDEYVCQLHKF 131

Query: 125 AEELLDMLCENLGLEKGYLKKAFYGSQGPTFGTKVSNYPPCPKPELIKGLRAHTDAGGII 184
           AE L  ++CENLGL++  +  AF G +GP FGTKV+ YP CP+PEL++GLR HTDAGGII
Sbjct: 132 AERLSKLMCENLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHTDAGGII 191

Query: 185 LLFQDDKVSGLQLLKDGNWIDVPPMR-HAIVVNLGDQLEVITNGRYKSVMHRVITQANGT 244
           LL QDD+V GL+  KDG W+ +PP + + I VN GDQLE+++NGRYKSV+HRV+T  +G+
Sbjct: 192 LLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVMTVKHGS 251

Query: 245 GRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPK-FVFEDYMKLYLGVKFEAKEPRF 304
            R+SIA+FYNP  DA+I PAP L          +YP  + F+DY+KLY   KF  K PR 
Sbjct: 252 -RLSIATFYNPAGDAIISPAPKL----------LYPSGYRFQDYLKLYSTTKFGDKGPRL 306

Query: 305 EAMKANAN 311
           E MK   N
Sbjct: 312 ETMKKMGN 306

[Close]

BLAST of MC04g0085 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 300.1 bits (767), Expect = 2.2e-81
Identity = 161/317 (50.79%), Postives = 212/317 (66.88%), Query Frame = 0


Query: 1   MAIFPVINLDSINGDARPKILEQIEDACRNWGFFEVVNHGIPHEFLDRVEKMTRDHYKKC 60
           MAI PVI+   +NG+ R K L +I  AC  WGFF++VNHGIP E L++V+K++ D YK  
Sbjct: 1   MAI-PVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTE 60

Query: 61  MEERFKETVASKGL-----EAAQAEVNDMDWESTFFLRHLPQSNISEIPDLDDEYRKVMK 120
            EE FK +   K L     + +  ++ ++DWE  F L      N +E P      ++ M 
Sbjct: 61  REEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLL---DHNQNEWP---SNIKETMG 120

Query: 121 EFAKLLEKLAEELLDMLCENLGLEKGYLKKAFY-----GSQGPTFGTKVSNYPPCPKPEL 180
           E+ + + KLA ++++++ ENLGL KGY+KKAF      G +   FGTKVS+YPPCP PEL
Sbjct: 121 EYREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPEL 180

Query: 181 IKGLRAHTDAGGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYK 240
           + GLRAHTDAGG++LLFQDD+  GLQ+LKDG WIDV P+ +AIV+N GDQ+EV++NGRYK
Sbjct: 181 VNGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYK 240

Query: 241 SVMHRVITQANGTGRMSIASFYNPGSDAVIFPAPALVEKEAEEKNGVYPKFVFEDYMKLY 300
           S  HRV+ +  G  R SIASFYNP   A I PA A+ E+E  EK   YPKFVF DYM +Y
Sbjct: 241 SAWHRVLAREEG-NRRSIASFYNPSYKAAIGPA-AVAEEEGSEKK--YPKFVFGDYMDVY 300

Query: 301 LGVKFEAKEPRFEAMKA 308
              KF  KEPRF A+K+
Sbjct: 301 ANQKFMPKEPRFLAVKS 306

The following BLAST results are available for this feature:

BLAST of MC04g0085 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q04644	1.6e-169	89.62	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=ACO1 PE=2...	[more]
Q9MB94	3.7e-150	78.44	1-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume OX=102107 GN=ACO1 PE=2 ...	[more]
Q08507	8.1e-150	79.13	1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 P...	[more]
P05116	1.8e-149	79.50	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=A...	[more]
Q08506	9.0e-149	79.00	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 P...	[more]

BLAST of MC04g0085 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148024.1	2.05e-234	100.00	1-aminocyclopropane-1-carboxylate oxidase 1 [Momordica charantia]	[more]
AFF19571.1	1.38e-232	99.37	1-aminocyclopropane-1-carboxylate oxidase [Momordica charantia]	[more]
AEM42994.1	1.99e-216	91.17	ACC oxidase [Siraitia grosvenorii]	[more]
KAA0061654.1	1.14e-213	89.94	1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa] >TYK05852...	[more]
NP_001284392.1	1.33e-212	89.62	1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo] >Q04644.1 RecName: Fu...	[more]

BLAST of MC04g0085 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2S4	9.90e-235	100.00	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Momordica charantia OX=3673 GN=LO...	[more]
H9NAH9	6.69e-233	99.37	1-aminocyclopropane-1-carboxylate oxidase OS=Momordica charantia OX=3673 PE=2 SV...	[more]
K7NBW2	9.64e-217	91.17	ACC oxidase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
A0A5D3C3Q0	5.54e-214	89.94	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo var. makuwa OX=11946...	[more]
D0V4H6	6.46e-213	89.62	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103491...	[more]

BLAST of MC04g0085 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05010.1	5.2e-144	73.37	ethylene-forming enzyme	[more]
AT1G62380.1	2.7e-132	68.57	ACC oxidase 2	[more]
AT1G12010.1	6.0e-132	68.89	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G19590.1	9.4e-85	48.70	ACC oxidase 1	[more]
AT1G77330.1	2.2e-81	50.79	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 159..254 e-value: 8.0E-28 score: 96.9
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..316 e-value: 1.8E-102 score: 344.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 5..95 e-value: 5.8E-15 score: 56.1
None	No IPR available	PANTHER	PTHR47990:SF63	ACC OXIDASE 2	coord: 4..307
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..307
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 4..305
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 153..254 score: 13.181551

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0085.1	MC04g0085.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009693	ethylene biosynthetic process
biological_process	GO:0009835	fruit ripening
molecular_function	GO:0009815	1-aminocyclopropane-1-carboxylate oxidase activity
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0046872	metal ion binding