MC04g0083 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0083 vs. ExPASy Swiss-Prot
Match: Q6YSJ5 (Protein argonaute 16 OS=Oryza sativa subsp. japonica OX=39947 GN=AGO16 PE=2 SV=2)

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 587/875 (67.09%), Postives = 708/875 (80.91%), Query Frame = 0

Query: 33  PPKYSIMSRRGVGSKGRRIPLLTNHFKVSVNAPDLIFYQYSVSISYEDNRPVEGKGIGRK 92
           P K   ++R   G +G++I LL+NHF V ++  D +FYQYSVSI  ED++ ++GKGIGRK
Sbjct: 16  PVKRVPIARPSFGREGKQIKLLSNHFTVKLSGIDAVFYQYSVSIKSEDDKVIDGKGIGRK 75

Query: 93  LMDQLYQTYSAELANRRFAYDGEKTLFSIGPLPQNKLEFTVVLEGSYAKLETGSSGGNGS 152
           +MD++ QTYS+ELA + FAYDGEK LF++GPLPQN  EFTV+LE + ++   G S G+GS
Sbjct: 76  VMDKVLQTYSSELAGKEFAYDGEKCLFTVGPLPQNNFEFTVILEETSSR-AAGGSLGHGS 135

Query: 153 PN-GIGKRSKRSFQLKTFKVELSFATKIPMKSIFTALKGSEVDNGSTQDALRVLDIILRQ 212
           PN G  KRSK +   K   V +S+A KIP+KS+  AL+GSE D+   QDALRVLDI+LRQ
Sbjct: 136 PNQGDKKRSKCTHLAKKIVVGISYAAKIPLKSVALALQGSESDH--AQDALRVLDIVLRQ 195

Query: 213 QAANRGCLLVRQSFFHDDSRNFVDVGGGVTGVRGFHSSFRLAQDGLSLNMDVSTTMILKP 272
           Q A RGCLLVRQSFF DD RN VD+ GGV+G RG HSSFR    GLSLNMDVSTTMI+ P
Sbjct: 196 QQAKRGCLLVRQSFFSDDFRNLVDLTGGVSGCRGLHSSFRTTIGGLSLNMDVSTTMIVTP 255

Query: 273 GPVIDFLIANQNVREPRYIDWAKAKRMLKNLRVKARHRNMEFKIIGLSEKPCNQQFFSMK 332
           GPV DFL+ NQNVR+ R IDW +AK+MLKNLRVKA H NMEFKIIGLS++PC++Q F MK
Sbjct: 256 GPVFDFLLTNQNVRDIRDIDWPRAKKMLKNLRVKAIHNNMEFKIIGLSDEPCSRQTFPMK 315

Query: 333 LKNNSSTDEQMVDITVHEYFVRHCGIELTYSAYLPCLDVGKPKRPNYLPLELCSLVSLQR 392
           ++N SS  E  V+ITV EYF +   ++LT   YLPCLDVGKPKRPNY+P+ELC +VSLQR
Sbjct: 316 VRNGSSEGE-TVEITVQEYF-KSKQVDLT-MPYLPCLDVGKPKRPNYVPIELCHMVSLQR 375

Query: 393 YTKALSSIQRASLVEKSRQKPQEKIRIVTDALKNYRYDEDPVLAQCVLKIDKQLTQLEGR 452
           YTKALSS QRA+LVEKSRQKPQE++R+VTDA+KN RYD+DP+L+ C +KI+KQLT+++GR
Sbjct: 376 YTKALSSQQRATLVEKSRQKPQERMRVVTDAVKNNRYDDDPILSSCGIKIEKQLTRVDGR 435

Query: 453 VLESPKLKVGKSDDCIPRNGRWNFNNKTLLNPTRIDRWVVVNFSARCDTSFISRELINCG 512
           VL +P L VG S+DCIP  GRWN+NNK L  P +I+RW +VNFSARCD S ISR+LINCG
Sbjct: 436 VLSAPTLVVGNSEDCIPNRGRWNYNNKRLFEPVKIERWAIVNFSARCDMSRISRDLINCG 495

Query: 513 RNKGIHIERPCTLIEEDQHSRRASPVDRVENMFEQIITKLPDKPPSFILCVLPEKKNSNI 572
           R KGI IERP TL++ED  SRR +PV RVE+MFE++   LP  PP F+LCVLPE+KN ++
Sbjct: 496 RTKGIIIERPFTLVDEDSQSRRCTPVVRVESMFEKVKANLPG-PPEFLLCVLPERKNCDL 555

Query: 573 YGPWKKKCLCDFGIATQCISPT-KINDQYITNVLLKINSKLGGINSLLAIEHASCVPLIK 632
           YGPWKKK L + GI TQCI P+ K+NDQY TNVLLKIN+KLGG+NS L++EH   +P++ 
Sbjct: 556 YGPWKKKNLHEMGIITQCIVPSVKMNDQYYTNVLLKINAKLGGMNSKLSLEHRHMIPIVN 615

Query: 633 DTPTLILGMDVSHGSPGRSDVPSIAAVVGSRSWPLISRYRAAVRTQSPKVEMIDGLFKPL 692
            TPTLILGMDVSHGSPGR+DVPSIAAVVGSR WPLISRYRA+VRTQSPKVEMID LFKPL
Sbjct: 616 QTPTLILGMDVSHGSPGRADVPSIAAVVGSRCWPLISRYRASVRTQSPKVEMIDSLFKPL 675

Query: 693 EGDQDDGIIRELLLDFYSTSNGRKPTQIILFRDGVSESQFNQVLNIELDQIVKAYQHLGE 752
           +  +DDGIIRELLLDFY TS  RKP QII+FRDGVSESQF+QVLN+EL+QI+KAYQ++ +
Sbjct: 676 DDGKDDGIIRELLLDFYKTSQQRKPKQIIIFRDGVSESQFSQVLNVELNQIIKAYQYMDQ 735

Query: 753 ANVPKFTVIVGQKNHHTKFFQAGAPENVPPGTVVDTKVVHPRNYDFYMCAHAGMIGTSRP 812
             +PKFTVI+ QKNHHTK FQ   P+NVPPGTVVD+ +VHPR YDFYM AHAG IGTSRP
Sbjct: 736 GPIPKFTVIIAQKNHHTKLFQENTPDNVPPGTVVDSGIVHPRQYDFYMYAHAGPIGTSRP 795

Query: 813 AHYHVLVDEIGFSPDDLQNLIHSLSYVYQRSTTALSIVAPICYAHLAASQMSQFIKFEDL 872
            HYHVL+DEIGF PDD+Q L+ SLSYVYQRSTTA+S+VAPICYAHLAA+QM QF+KFE+ 
Sbjct: 796 THYHVLLDEIGFLPDDVQKLVLSLSYVYQRSTTAISVVAPICYAHLAAAQMGQFMKFEEF 855

Query: 873 SETSSERGTVTSAGSLSIPELPRLDKKVAGSMFFC 906
           +ETSS  G V S+    +PELPRL   V  SMFFC
Sbjct: 856 AETSSGSGGVPSSSGAVVPELPRLHADVCSSMFFC 883

BLAST of MC04g0083 vs. ExPASy Swiss-Prot
Match: Q9SDG8 (Protein argonaute 4A OS=Oryza sativa subsp. japonica OX=39947 GN=AGO4A PE=2 SV=1)

HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 567/904 (62.72%), Postives = 703/904 (77.77%), Query Frame = 0

Query: 10  NSEASPLPLGPPVPPNMK------LEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKVSVN 69
           + E   LP  PP+PPN +       +K   PK ++M+R G G KG+ I LLTNHFKVS+ 
Sbjct: 5   SGEIEELPPPPPLPPNAEPIKTDDTKKLSKPKRALMARSGCGKKGQPIQLLTNHFKVSLK 64

Query: 70  APDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFSIGP 129
           A D  F+ Y V++ YED+RPV+GKGIGRK++D+L QTY++ELAN+ FAYDGEK+LF+IG 
Sbjct: 65  AADEFFHHYYVNLKYEDDRPVDGKGIGRKVLDKLQQTYASELANKDFAYDGEKSLFTIGA 124

Query: 130 LPQNKLEFTVVLE--GSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIPM 189
           LPQ   EFTVVLE   +      G S GN SP    KR +R +Q KTFKVEL+FA KIPM
Sbjct: 125 LPQVNNEFTVVLEDFNTGKSSANGGSPGNDSPGNDRKRVRRPYQTKTFKVELNFAAKIPM 184

Query: 190 KSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGVT 249
            +I  AL+G E +N  TQ+A+RV+DIILRQ +A +GCLLVRQSFFH++  NFVD+GGGV 
Sbjct: 185 SAIAQALRGQESEN--TQEAIRVIDIILRQHSAKQGCLLVRQSFFHNNPSNFVDLGGGVM 244

Query: 250 GVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLKN 309
           G RGFHSSFR  Q GLSLN+DVSTTMI+KPGPV+DFL+ANQ V  P  IDWAKAKR LKN
Sbjct: 245 GCRGFHSSFRATQSGLSLNIDVSTTMIVKPGPVVDFLLANQKVDHPNKIDWAKAKRALKN 304

Query: 310 LRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELTY 369
           LR+K    N E+KI+GLSE+ C +Q F++K +N     E  V+++V+EYFV++ GIEL Y
Sbjct: 305 LRIKTSPANTEYKIVGLSERNCYEQMFTLKQRNGDGEPEG-VEVSVYEYFVKNRGIELRY 364

Query: 370 SAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVTD 429
           S   PC++VGKPKRP Y P+ELCSLV LQRYTKALS++QR+SLVEKSRQKP+E++ +++D
Sbjct: 365 SGDFPCINVGKPKRPTYFPIELCSLVPLQRYTKALSTLQRSSLVEKSRQKPEERMSVLSD 424

Query: 430 ALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTLL 489
            LK   YD +P+L  C + I +  TQ+ GRVL++PKLK G  +D   RNGRWNFNNK L+
Sbjct: 425 VLKRSNYDSEPMLNSCGISIARGFTQVAGRVLQAPKLKAGNGEDLFARNGRWNFNNKRLI 484

Query: 490 NPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRVE 549
             + I++W VVNFSARC+   + R++I CG  KGI +E P  +IEED   RRA    RV+
Sbjct: 485 KASSIEKWAVVNFSARCNIRDLVRDIIKCGGMKGIKVEDPFDVIEEDPSMRRAPAARRVD 544

Query: 550 NMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYIT 609
            M +++  KLP + P F+LCVL E+KNS+IYGPWK+KCL +FGI TQC++PT++NDQYIT
Sbjct: 545 GMIDKMQKKLPGQ-PKFLLCVLAERKNSDIYGPWKRKCLAEFGIITQCVAPTRVNDQYIT 604

Query: 610 NVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGSR 669
           NVLLKIN+KLGG+NSLL IE +  +PL+   PT+ILGMDVSHGSPG+SD+PSIAAVV SR
Sbjct: 605 NVLLKINAKLGGLNSLLQIETSPSIPLVSKVPTIILGMDVSHGSPGQSDIPSIAAVVSSR 664

Query: 670 SWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIILF 729
            WPL+S+YRA+VR+QSPK+EMIDGLFKP    +DDG+IRELL+DFY+++  RKP Q+I+F
Sbjct: 665 EWPLVSKYRASVRSQSPKLEMIDGLFKPQGAQEDDGLIRELLVDFYTSTGKRKPDQVIIF 724

Query: 730 RDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPPG 789
           RDGVSESQF QVLNIELDQI++A + L E   PKFT+IV QKNHHTKFF  G+  NVPPG
Sbjct: 725 RDGVSESQFTQVLNIELDQIIEACKFLDENWSPKFTLIVAQKNHHTKFFVPGSQNNVPPG 784

Query: 790 TVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQRS 849
           TVVD  V HPRN DFYMCAHAGMIGT+RP HYH+L DEIGFS DDLQ L+HSLSYVYQRS
Sbjct: 785 TVVDNAVCHPRNNDFYMCAHAGMIGTTRPTHYHILHDEIGFSADDLQELVHSLSYVYQRS 844

Query: 850 TTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAGS 906
           TTA+S+VAPICYAHLAA+Q+SQFIKF+++SETSS  G  TSAGS  +PELPRL  KV  S
Sbjct: 845 TTAISVVAPICYAHLAAAQVSQFIKFDEMSETSSSHGGHTSAGSAPVPELPRLHNKVRSS 904

BLAST of MC04g0083 vs. ExPASy Swiss-Prot
Match: Q0JF58 (Protein argonaute 4B OS=Oryza sativa subsp. japonica OX=39947 GN=AGO4B PE=2 SV=1)

HSP 1 Score: 1142.9 bits (2955), Expect = 0.0e+00
Identity = 566/911 (62.13%), Postives = 701/911 (76.95%), Query Frame = 0

Query: 10  NSEASPLPLGPPVPPNM------KLEKAVP------PKYSIMSRRGVGSKGRRIPLLTNH 69
           + EA  LP  PPVP N+       +E  VP      PK   M+R G+G KG+ I LL NH
Sbjct: 5   DGEADELPPPPPVPANVVPIKADDVESEVPANKPAKPKRFPMARPGLGRKGQPIQLLANH 64

Query: 70  FKVSVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKT 129
           +KVSV + +  F+ Y+V + YED+RPV+GKG+GRK++D+L QTY +EL+++ FAYDGEK+
Sbjct: 65  YKVSVKSSEEYFFHYNVILKYEDDRPVDGKGVGRKVIDKLQQTYRSELSSKDFAYDGEKS 124

Query: 130 LFSIGPLPQNKLEFTVVLE--GSYAKLETGSSGGNGSPNGIG-KRSKRSFQLKTFKVELS 189
           LF+IG LPQ   EFTVVLE   +      GS GGN SP G   KR +R +Q KTFKVEL 
Sbjct: 125 LFTIGALPQVTNEFTVVLEDVSTGKTAANGSPGGNDSPGGSDRKRVRRPYQTKTFKVELC 184

Query: 190 FATKIPMKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFV 249
           FA KIPM +I  A+KG E +N  +Q+ALRVLDIILRQ +A +GCLLVRQSFFH++  NFV
Sbjct: 185 FAAKIPMNAIAQAIKGQESEN--SQEALRVLDIILRQHSAKQGCLLVRQSFFHNNPNNFV 244

Query: 250 DVGGGVTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAK 309
           D+GGGV G RGFHSSFR  Q GLSLN+DVSTTMI+KPGPVIDFL+ANQ V  P  IDW K
Sbjct: 245 DLGGGVMGCRGFHSSFRGTQSGLSLNIDVSTTMIVKPGPVIDFLLANQKVDHPDRIDWQK 304

Query: 310 AKRMLKNLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRH 369
           AKR LKNLR++    N EFKIIGLS++ CN+Q FS++ +N ++ D   V++TV++YFV++
Sbjct: 305 AKRALKNLRIRTTPVNSEFKIIGLSDRNCNEQMFSLRQRNGNNGDVDEVEVTVYDYFVKN 364

Query: 370 CGIELTYSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQE 429
            GIEL YS  LPC++VGKPKRP Y P+ELCSL+ LQRYTKALS++QR+SLVEKSRQKPQE
Sbjct: 365 KGIELRYSGNLPCINVGKPKRPTYFPIELCSLIPLQRYTKALSTLQRSSLVEKSRQKPQE 424

Query: 430 KIRIVTDALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWN 489
           ++ ++ DAL++  YD DP+L    + I +  TQ+EGRVL+ PKLK G  +D  PRNGRWN
Sbjct: 425 RMSVLNDALRHSNYDSDPMLRASGISIAQNFTQVEGRVLQPPKLKAGNGEDIFPRNGRWN 484

Query: 490 FNNKTLLNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRA 549
           FNNK L+    +D+W VVNFSARCD   + R+LI     KGI +  P  + EE    RRA
Sbjct: 485 FNNKKLIQTCSVDKWAVVNFSARCDVRNLIRDLIRNASAKGIQMAEPFDVFEESPSLRRA 544

Query: 550 SPVDRVENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTK 609
               RV++MFEQI +KLP   P F+LC+LPE+KN  +YGPWK+KCL +FGI TQC++P +
Sbjct: 545 PVSRRVDDMFEQIKSKLPG-APKFLLCLLPERKNCEVYGPWKRKCLAEFGIVTQCLAPQR 604

Query: 610 INDQYITNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSI 669
           +NDQY+ N+LLKIN+KLGGINSLL IE +  +PL+  TPT+ILGMDVSHG PG+SD PSI
Sbjct: 605 VNDQYLLNLLLKINAKLGGINSLLQIEASPSIPLVSKTPTIILGMDVSHGQPGQSDRPSI 664

Query: 670 AAVVGSRSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRK 729
           AAVV SR WPLIS+YRA+V TQSPK+EM+  LFKP  G +DDG+IRE L+DFY++S  RK
Sbjct: 665 AAVVSSRQWPLISKYRASVHTQSPKLEMMSSLFKP-RGTEDDGLIRESLIDFYTSSGKRK 724

Query: 730 PTQIILFRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGA 789
           P  +I+FRDGVSESQF QV+NIELDQI++A + L E   PKFTVIV QKNHHTKFFQ+G+
Sbjct: 725 PDHVIVFRDGVSESQFTQVINIELDQIIEACKFLDEKWSPKFTVIVAQKNHHTKFFQSGS 784

Query: 790 PENVPPGTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSL 849
           P+NVPPGTVVD +V HPRNYDFYMCAHAGMIGT+RP HYHVL DEIGFSPDDLQ L+HSL
Sbjct: 785 PDNVPPGTVVDKQVCHPRNYDFYMCAHAGMIGTTRPTHYHVLHDEIGFSPDDLQELVHSL 844

Query: 850 SYVYQRSTTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRL 906
           SYVYQRSTTA+S+VAPICYAHLAA+Q+  F+KFED+S+ SS +G  TS GS+ +PELPRL
Sbjct: 845 SYVYQRSTTAISVVAPICYAHLAAAQVGTFLKFEDMSDASSSQGGHTSVGSVPVPELPRL 904

BLAST of MC04g0083 vs. ExPASy Swiss-Prot
Match: O48771 (Protein argonaute 6 OS=Arabidopsis thaliana OX=3702 GN=AGO6 PE=2 SV=2)

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 572/895 (63.91%), Postives = 692/895 (77.32%), Query Frame = 0

Query: 13  ASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKVSVNAPDLIFYQY 72
           +S LPL    P +++ E+     Y I +RRGVG+ G  I L TNHF VSV  PD++FYQY
Sbjct: 4   SSSLPLS---PISIEPEQPSHRDYDITTRRGVGTTGNPIELCTNHFNVSVRQPDVVFYQY 63

Query: 73  SVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFSIGPLPQNKLEFT 132
           +VSI+ E+   V+G GI RKLMDQL++TYS++L  +R AYDGEKTL+++GPLPQN+ +F 
Sbjct: 64  TVSITTENGDAVDGTGISRKLMDQLFKTYSSDLDGKRLAYDGEKTLYTVGPLPQNEFDFL 123

Query: 133 VVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIPMKSIFTALKGSE 192
           V++EGS++K + G S G GS +G  KRSKRSF  +++KV++ +A +IP+K++    +G+ 
Sbjct: 124 VIVEGSFSKRDCGVSDG-GSSSGTCKRSKRSFLPRSYKVQIHYAAEIPLKTVLGTQRGAY 183

Query: 193 VDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGVTGVRGFHSSFRL 252
             + S QDALRVLDI+LRQQAA RGCLLVRQ+FFH D    + VGGGV G+RG HSSFR 
Sbjct: 184 TPDKSAQDALRVLDIVLRQQAAERGCLLVRQAFFHSDGHP-MKVGGGVIGIRGLHSSFRP 243

Query: 253 AQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLKNLRVKARHRNME 312
              GLSLN+DVSTTMIL+PGPVI+FL ANQ+V  PR IDW KA +MLK++RVKA HRNME
Sbjct: 244 THGGLSLNIDVSTTMILEPGPVIEFLKANQSVETPRQIDWIKAAKMLKHMRVKATHRNME 303

Query: 313 FKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELTYSAYLPCLDVGK 372
           FKIIGLS KPCNQQ FSMK+K +   +  + +ITV++YF +    E   SAY PCLDVGK
Sbjct: 304 FKIIGLSSKPCNQQLFSMKIK-DGEREVPIREITVYDYF-KQTYTEPISSAYFPCLDVGK 363

Query: 373 PKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVTDALKNYRYDEDP 432
           P RPNYLPLE C+LVSLQRYTK LS  QR  LVE SRQKP E+I+ + DA+  Y YD+DP
Sbjct: 364 PDRPNYLPLEFCNLVSLQRYTKPLSGRQRVLLVESSRQKPLERIKTLNDAMHTYCYDKDP 423

Query: 433 VLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTLLNPTRIDRWVVV 492
            LA C + I+K++TQ+EGRVL+ P LK GK++D  P NGRWNFNNK LL P  I  W +V
Sbjct: 424 FLAGCGISIEKEMTQVEGRVLKPPMLKFGKNEDFQPCNGRWNFNNKMLLEPRAIKSWAIV 483

Query: 493 NFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRVENMFEQIITKLP 552
           NFS  CD+S ISRELI+CG  KGI I+RP  L+EED   ++A PV+RVE M   +  K P
Sbjct: 484 NFSFPCDSSHISRELISCGMRKGIEIDRPFALVEEDPQYKKAGPVERVEKMIATMKLKFP 543

Query: 553 DKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYITNVLLKINSKLG 612
           D PP FILC+LPE+K S+IYGPWKK CL + GI TQCI P KI+DQY+TNVLLKINSKLG
Sbjct: 544 D-PPHFILCILPERKTSDIYGPWKKICLTEEGIHTQCICPIKISDQYLTNVLLKINSKLG 603

Query: 613 GINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGSRSWPLISRYRAA 672
           GINSLL IE++  +PLI   PTLILGMDVSHG PGR+DVPS+AAVVGS+ WPLISRYRAA
Sbjct: 604 GINSLLGIEYSYNIPLINKIPTLILGMDVSHGPPGRADVPSVAAVVGSKCWPLISRYRAA 663

Query: 673 VRTQSPKVEMIDGLFKPLEGDQ--DDGIIRELLLDFYSTSNGRKPTQIILFRDGVSESQF 732
           VRTQSP++EMID LF+P+E  +  D+GI+ EL ++FY TS  RKP QII+FRDGVSESQF
Sbjct: 664 VRTQSPRLEMIDSLFQPIENTEKGDNGIMNELFVEFYRTSRARKPKQIIIFRDGVSESQF 723

Query: 733 NQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPPGTVVDTKVVH 792
            QVL IE+DQI+KAYQ LGE++VPKFTVIV QKNHHTK FQA  PENVP GTVVDTK+VH
Sbjct: 724 EQVLKIEVDQIIKAYQRLGESDVPKFTVIVAQKNHHTKLFQAKGPENVPAGTVVDTKIVH 783

Query: 793 PRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQRSTTALSIVAP 852
           P NYDFYMCAHAG IGTSRPAHYHVL+DEIGFSPDDLQNLIHSLSYV QRSTTA SIVAP
Sbjct: 784 PTNYDFYMCAHAGKIGTSRPAHYHVLLDEIGFSPDDLQNLIHSLSYVNQRSTTATSIVAP 843

Query: 853 ICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAGSMFFC 906
           + YAHLAA+Q++QF KFE +SE               +PELPRL + V G+MFFC
Sbjct: 844 VRYAHLAAAQVAQFTKFEGISEDG------------KVPELPRLHENVEGNMFFC 878

BLAST of MC04g0083 vs. ExPASy Swiss-Prot
Match: Q9ZVD5 (Protein argonaute 4 OS=Arabidopsis thaliana OX=3702 GN=AGO4 PE=1 SV=2)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 544/919 (59.19%), Postives = 691/919 (75.19%), Query Frame = 0

Query: 3   NITNAGGNSEASPLPLGPPVPPNM-------KLEKAVPPKYSIMSRRGVGSKGRRIPLLT 62
           N  N  G +EA P P  P +PPN+       +L +   P    M+R+G G++G++IPLLT
Sbjct: 15  NGANGSGVTEALP-PPPPVIPPNVEPVRVKTELAEKKGPVRVPMARKGFGTRGQKIPLLT 74

Query: 63  NHFKVSVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGE 122
           NHFKV V      F+ YSV++ Y+D RPVE KG+GRK++D+++QTY ++L  + FAYDGE
Sbjct: 75  NHFKVDVANLQGHFFHYSVALFYDDGRPVEQKGVGRKILDKVHQTYHSDLDGKEFAYDGE 134

Query: 123 KTLFSIGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPN-------GIGKRSKRSFQLKT 182
           KTLF+ G LP NK++F+VVLE    ++    + GNGSPN       G  KR +R  + K 
Sbjct: 135 KTLFTYGALPSNKMDFSVVLE----EVSATRANGNGSPNGNESPSDGDRKRLRRPNRSKN 194

Query: 183 FKVELSFATKIPMKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHD 242
           F+VE+S+A KIP++++  A++G E +N  +Q+A+RVLDIILRQ AA +GCLLVRQSFFH+
Sbjct: 195 FRVEISYAAKIPLQALANAMRGQESEN--SQEAIRVLDIILRQHAARQGCLLVRQSFFHN 254

Query: 243 DSRNFVDVGGGVTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPR 302
           D  N   VGG + G RGFHSSFR  Q G+SLNMDV+TTMI+KPGPV+DFLIANQN R+P 
Sbjct: 255 DPTNCEPVGGNILGCRGFHSSFRTTQGGMSLNMDVTTTMIIKPGPVVDFLIANQNARDPY 314

Query: 303 YIDWAKAKRMLKNLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKN-NSSTDEQMVDITV 362
            IDW+KAKR LKNLRVK      EFKI GLS+KPC +Q F +K +N N + + +  ++TV
Sbjct: 315 SIDWSKAKRTLKNLRVKVSPSGQEFKITGLSDKPCREQTFELKKRNPNENGEFETTEVTV 374

Query: 363 HEYFVRHCGIELTYSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEK 422
            +YF     I+L YSA LPC++VGKPKRP Y+PLELC+LV LQRYTKAL++ QR++LVEK
Sbjct: 375 ADYFRDTRHIDLQYSADLPCINVGKPKRPTYIPLELCALVPLQRYTKALTTFQRSALVEK 434

Query: 423 SRQKPQEKIRIVTDALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCI 482
           SRQKPQE++ +++ ALK   YD +P+L  C + I    TQ+EGRVL +PKLK+G   +  
Sbjct: 435 SRQKPQERMTVLSKALKVSNYDAEPLLRSCGISISSNFTQVEGRVLPAPKLKMGCGSETF 494

Query: 483 PRNGRWNFNNKTLLNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEE 542
           PRNGRWNFNNK  + PT+I RWVVVNFSARC+   +  +LI  G +KGI I  P  + EE
Sbjct: 495 PRNGRWNFNNKEFVEPTKIQRWVVVNFSARCNVRQVVDDLIKIGGSKGIEIASPFQVFEE 554

Query: 543 DQHSRRASPVDRVENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIAT 602
               RRA P+ RVENMF+ I +KLP   P FILCVLP+KKNS++YGPWKKK L +FGI T
Sbjct: 555 GNQFRRAPPMIRVENMFKDIQSKLPG-VPQFILCVLPDKKNSDLYGPWKKKNLTEFGIVT 614

Query: 603 QCISPTK-INDQYITNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSP 662
           QC++PT+  NDQY+TN+LLKIN+KLGG+NS+L++E      +I   PT+ILGMDVSHGSP
Sbjct: 615 QCMAPTRQPNDQYLTNLLLKINAKLGGLNSMLSVERTPAFTVISKVPTIILGMDVSHGSP 674

Query: 663 GRSDVPSIAAVVGSRSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDF 722
           G+SDVPSIAAVV SR WPLIS+YRA+VRTQ  K EMI+ L K   G +DDGII+ELL+DF
Sbjct: 675 GQSDVPSIAAVVSSREWPLISKYRASVRTQPSKAEMIESLVKK-NGTEDDGIIKELLVDF 734

Query: 723 YSTSNGRKPTQIILFRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHH 782
           Y++SN RKP  II+FRDGVSESQFNQVLNIELDQI++A + L     PKF ++V QKNHH
Sbjct: 735 YTSSNKRKPEHIIIFRDGVSESQFNQVLNIELDQIIEACKLLDANWNPKFLLLVAQKNHH 794

Query: 783 TKFFQAGAPENVPPGTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDD 842
           TKFFQ  +PENVPPGT++D K+ HP+N DFY+CAHAGMIGT+RP HYHVL DEIGFS D+
Sbjct: 795 TKFFQPTSPENVPPGTIIDNKICHPKNNDFYLCAHAGMIGTTRPTHYHVLYDEIGFSADE 854

Query: 843 LQNLIHSLSYVYQRSTTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSL 902
           LQ L+HSLSYVYQRST+A+S+VAPICYAHLAA+Q+  F+KFED SETSS  G +T+ G +
Sbjct: 855 LQELVHSLSYVYQRSTSAISVVAPICYAHLAAAQLGTFMKFEDQSETSSSHGGITAPGPI 914

Query: 903 SIPELPRLDKKVAGSMFFC 906
           S+ +LPRL   VA SMFFC
Sbjct: 915 SVAQLPRLKDNVANSMFFC 924

BLAST of MC04g0083 vs. NCBI nr
Match: XP_022147942.1 (protein argonaute 16 [Momordica charantia])

HSP 1 Score: 1811 bits (4692), Expect = 0.0
Identity = 905/905 (100.00%), Postives = 905/905 (100.00%), Query Frame = 0

Query: 1   MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV 60
           MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV
Sbjct: 1   MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV 60

Query: 61  SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS 120
           SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS
Sbjct: 61  SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS 120

Query: 121 IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP 180
           IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP
Sbjct: 121 IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP 180

Query: 181 MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV 240
           MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV
Sbjct: 181 MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV 240

Query: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK 300
           TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK
Sbjct: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK 300

Query: 301 NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT 360
           NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT
Sbjct: 301 NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT 360

Query: 361 YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT 420
           YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT
Sbjct: 361 YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT 420

Query: 421 DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL 480
           DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL
Sbjct: 421 DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL 480

Query: 481 LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV 540
           LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV
Sbjct: 481 LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV 540

Query: 541 ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI 600
           ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI
Sbjct: 541 ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI 600

Query: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660
           TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS
Sbjct: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660

Query: 661 RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL 720
           RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL
Sbjct: 661 RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL 720

Query: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP 780
           FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP
Sbjct: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP 780

Query: 781 GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840
           GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR
Sbjct: 781 GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840

Query: 841 STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG 900
           STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG
Sbjct: 841 STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG 900

Query: 901 SMFFC 905
           SMFFC
Sbjct: 901 SMFFC 905

BLAST of MC04g0083 vs. NCBI nr
Match: XP_022988650.1 (protein argonaute 16 [Cucurbita maxima] >XP_022988651.1 protein argonaute 16 [Cucurbita maxima])

HSP 1 Score: 1651 bits (4275), Expect = 0.0
Identity = 821/905 (90.72%), Postives = 858/905 (94.81%), Query Frame = 0

Query: 1   MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV 60
           MV+IT++ G SEA  LP G P+PP MK EKAVPP Y+IMSRRGVGSKGRRIPLL NHF+V
Sbjct: 1   MVDITHSEGESEAVALPPGTPIPPIMKPEKAVPPTYTIMSRRGVGSKGRRIPLLANHFRV 60

Query: 61  SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS 120
           SVNAPDLIFYQYSVSISYEDN+PVEGK IGRKLMD+LYQTYSAELAN+RFAYDGEK L+S
Sbjct: 61  SVNAPDLIFYQYSVSISYEDNKPVEGKEIGRKLMDKLYQTYSAELANKRFAYDGEKCLYS 120

Query: 121 IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP 180
           IGPLPQNK +FTVVLEGSYAK E+G+S  +GSPNG+GKRSK SFQ KTFKVELSFATKIP
Sbjct: 121 IGPLPQNKFDFTVVLEGSYAKPESGNSRSSGSPNGVGKRSKHSFQSKTFKVELSFATKIP 180

Query: 181 MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV 240
           MKSIFTALKGSEVDNG TQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNF D+GGGV
Sbjct: 181 MKSIFTALKGSEVDNGGTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFTDIGGGV 240

Query: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK 300
           TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPG VIDFLIANQNVREPRYIDW KAKRMLK
Sbjct: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGSVIDFLIANQNVREPRYIDWVKAKRMLK 300

Query: 301 NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT 360
           NLRV ARHRNMEFKI GLSEKPCNQQFFSMKLKNN STD +M+DITV+EYFVRHCGIELT
Sbjct: 301 NLRVNARHRNMEFKITGLSEKPCNQQFFSMKLKNNGSTDGEMIDITVYEYFVRHCGIELT 360

Query: 361 YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT 420
           YSAYLPCLDVGK KRPNYLPLELCSLVSLQRYTKALSS+QRASLVEKSRQKPQEKI+IVT
Sbjct: 361 YSAYLPCLDVGKSKRPNYLPLELCSLVSLQRYTKALSSMQRASLVEKSRQKPQEKIKIVT 420

Query: 421 DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL 480
           DALK+YRYDEDPVLAQC LKIDKQLTQ+EGRVLESPKLKVG+SDDCIPRNGRWNFNNKTL
Sbjct: 421 DALKSYRYDEDPVLAQCGLKIDKQLTQVEGRVLESPKLKVGRSDDCIPRNGRWNFNNKTL 480

Query: 481 LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV 540
           LNPTRID W+VVNFSARCDTS+ISRELINCGRNKGIHIERP TLIEEDQHSRRASPVDRV
Sbjct: 481 LNPTRIDSWIVVNFSARCDTSYISRELINCGRNKGIHIERPITLIEEDQHSRRASPVDRV 540

Query: 541 ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI 600
           ENMFEQI+ KLP+ PPSFILCVLPEKKNSNIYGPWKKKCLCDFGI TQCISPTKINDQYI
Sbjct: 541 ENMFEQIMAKLPN-PPSFILCVLPEKKNSNIYGPWKKKCLCDFGIVTQCISPTKINDQYI 600

Query: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660
           TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS
Sbjct: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660

Query: 661 RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL 720
           RSWPLISRYRAAVRTQSPK+EMID L+KPLE   DDGIIRELLLDFY TSNGRKP QII+
Sbjct: 661 RSWPLISRYRAAVRTQSPKLEMIDALYKPLEDGNDDGIIRELLLDFYRTSNGRKPAQIIV 720

Query: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP 780
           FRDGVSESQFNQVLNIELDQIVKAYQHLGE NVPKFTVIVGQKNHHTKFFQAGAPENVPP
Sbjct: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEVNVPKFTVIVGQKNHHTKFFQAGAPENVPP 780

Query: 781 GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840
           GTVVDTKVVHP+NYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR
Sbjct: 781 GTVVDTKVVHPKNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840

Query: 841 STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG 900
           STTALSI APICYAHLAA+QMSQFIKFED SETSSE+G +TS+GS+SI ELPRL K V G
Sbjct: 841 STTALSIAAPICYAHLAAAQMSQFIKFEDHSETSSEQGAITSSGSVSILELPRLHKDVDG 900

Query: 901 SMFFC 905
           SMFFC
Sbjct: 901 SMFFC 904

BLAST of MC04g0083 vs. NCBI nr
Match: XP_022930861.1 (protein argonaute 16-like [Cucurbita moschata] >XP_022930862.1 protein argonaute 16-like [Cucurbita moschata])

HSP 1 Score: 1648 bits (4267), Expect = 0.0
Identity = 822/905 (90.83%), Postives = 856/905 (94.59%), Query Frame = 0

Query: 1   MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV 60
           MV+IT++ G SEA  LP   PVPP MK EKAVPP Y+IMSRRGVGSKGRRIPLLTNHF+V
Sbjct: 1   MVDITHSEGESEAVALPPDTPVPPIMKPEKAVPPTYTIMSRRGVGSKGRRIPLLTNHFRV 60

Query: 61  SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS 120
           SVNAPDLIFYQYSVSISYEDN+PVEGK IGRKLMD+LYQTYSAELAN+RFAYDGEK L+S
Sbjct: 61  SVNAPDLIFYQYSVSISYEDNKPVEGKEIGRKLMDKLYQTYSAELANKRFAYDGEKCLYS 120

Query: 121 IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP 180
           IGPLPQNK +FTVVLEGSYAK E+G+S  +GSPNG GKRSK SFQ KTFKVELSFATKIP
Sbjct: 121 IGPLPQNKFDFTVVLEGSYAKPESGNSRSSGSPNGAGKRSKHSFQSKTFKVELSFATKIP 180

Query: 181 MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV 240
           MKSIFTALKGSEVDNG TQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNF D+GGGV
Sbjct: 181 MKSIFTALKGSEVDNGGTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFTDIGGGV 240

Query: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK 300
           TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPG VIDFLIANQNVREPRYIDW KAKRMLK
Sbjct: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGSVIDFLIANQNVREPRYIDWVKAKRMLK 300

Query: 301 NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT 360
           NLRV ARHRNMEFKI GLSEKPCNQQFFSMKLKNN STD +M+DITV+EYFVRHCGIELT
Sbjct: 301 NLRVNARHRNMEFKITGLSEKPCNQQFFSMKLKNNGSTDGEMIDITVYEYFVRHCGIELT 360

Query: 361 YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT 420
           YSAYLPCLDVGK KRPNYLPLELCSLVSLQRYTKALSS+QRASLVEKSRQKPQEKI+IVT
Sbjct: 361 YSAYLPCLDVGKSKRPNYLPLELCSLVSLQRYTKALSSMQRASLVEKSRQKPQEKIKIVT 420

Query: 421 DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL 480
           DALK+YRYDEDPVLAQC LKIDKQLTQ+EGRVLESPKLKVG+SDDCIPRNGRWNFNNKTL
Sbjct: 421 DALKSYRYDEDPVLAQCGLKIDKQLTQVEGRVLESPKLKVGRSDDCIPRNGRWNFNNKTL 480

Query: 481 LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV 540
           LNPTRID W+VVNFSARCDTS+ISRELINCGRNKGIHIERP TLIEEDQHSRRASPVDRV
Sbjct: 481 LNPTRIDSWIVVNFSARCDTSYISRELINCGRNKGIHIERPITLIEEDQHSRRASPVDRV 540

Query: 541 ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI 600
           ENMFEQI+ KLP+ PPSFILCVLPEKKNSNIYGPWKKKCLCDFGI TQCISPTKINDQYI
Sbjct: 541 ENMFEQIMAKLPN-PPSFILCVLPEKKNSNIYGPWKKKCLCDFGIVTQCISPTKINDQYI 600

Query: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660
           TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS
Sbjct: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660

Query: 661 RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL 720
           RSWPLISRYRAAVRTQSPK+EMID L+KPLE   DDGIIRELLLDFY TSNGRKP QII+
Sbjct: 661 RSWPLISRYRAAVRTQSPKLEMIDALYKPLEDGNDDGIIRELLLDFYRTSNGRKPAQIIV 720

Query: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP 780
           FRDGVSESQFNQVLNIELDQIVKAYQHLGE NVPKFTVIVGQKNHHTKFFQAGAPENVPP
Sbjct: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEVNVPKFTVIVGQKNHHTKFFQAGAPENVPP 780

Query: 781 GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840
           GTVVDTKVVHP+NYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR
Sbjct: 781 GTVVDTKVVHPKNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840

Query: 841 STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG 900
           STTALSI APICYAHLAA+QMSQFIKFED SETSSE G +TS+GS+SI ELPRL K V G
Sbjct: 841 STTALSIAAPICYAHLAAAQMSQFIKFEDHSETSSELGAITSSGSVSILELPRLHKDVDG 900

Query: 901 SMFFC 905
           SMFFC
Sbjct: 901 SMFFC 904

BLAST of MC04g0083 vs. NCBI nr
Match: XP_023531841.1 (protein argonaute 16-like [Cucurbita pepo subsp. pepo] >XP_023531842.1 protein argonaute 16-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1647 bits (4266), Expect = 0.0
Identity = 820/905 (90.61%), Postives = 858/905 (94.81%), Query Frame = 0

Query: 1   MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV 60
           MV+IT++ G SEA  LP G PVPP MK EKAVPP Y+IMSRRGVGSKGRRIPLLTNHF+V
Sbjct: 1   MVDITHSEGESEAVALPPGTPVPPIMKPEKAVPPTYTIMSRRGVGSKGRRIPLLTNHFRV 60

Query: 61  SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS 120
           SVNAPDLIFYQYSV+ISYEDN+PVEGK IGRKLMD+LYQTYSAELAN+RFAYDGEK L+S
Sbjct: 61  SVNAPDLIFYQYSVTISYEDNKPVEGKEIGRKLMDKLYQTYSAELANKRFAYDGEKCLYS 120

Query: 121 IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP 180
           IGPLPQNK +FTVVLEGSYAK E+G+S  +GSPNG GKRSK SFQ KTFKVELSFATKIP
Sbjct: 121 IGPLPQNKFDFTVVLEGSYAKPESGNSRSSGSPNGAGKRSKHSFQSKTFKVELSFATKIP 180

Query: 181 MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV 240
           MKSIFTALKGSEVDNG TQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNF D+GGGV
Sbjct: 181 MKSIFTALKGSEVDNGGTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFTDIGGGV 240

Query: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK 300
           TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPG VIDFLIANQNVREPRYIDW KAKRMLK
Sbjct: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGSVIDFLIANQNVREPRYIDWVKAKRMLK 300

Query: 301 NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT 360
           NLRV ARHRNMEFKI GLSEKPCNQQFFSMKLKNN STD +M+DITV+EYFVRHCGIELT
Sbjct: 301 NLRVNARHRNMEFKITGLSEKPCNQQFFSMKLKNNGSTDGEMIDITVYEYFVRHCGIELT 360

Query: 361 YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT 420
           YSAYLPCLDVGK KRPNYLPLELCSLVSLQRYTKALSS+QRASLVEKSRQKPQEKI+IVT
Sbjct: 361 YSAYLPCLDVGKSKRPNYLPLELCSLVSLQRYTKALSSMQRASLVEKSRQKPQEKIKIVT 420

Query: 421 DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL 480
           DALK+YRYDEDPVLAQC LKIDKQLTQ+EGRVLESPKLKVG+SDDCIPRNGRWNFNNKTL
Sbjct: 421 DALKSYRYDEDPVLAQCGLKIDKQLTQVEGRVLESPKLKVGRSDDCIPRNGRWNFNNKTL 480

Query: 481 LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV 540
           LNPTRID W+VVNFSARCDTS+ISRELINCGRNKGIHIERP TLIEEDQHSRRASPVDRV
Sbjct: 481 LNPTRIDSWIVVNFSARCDTSYISRELINCGRNKGIHIERPITLIEEDQHSRRASPVDRV 540

Query: 541 ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI 600
           ENMFEQI+ KLP+ PPSFILCVLPEKKNS+IYGPWKKKCLCDFGI TQCISPTKINDQYI
Sbjct: 541 ENMFEQIMAKLPN-PPSFILCVLPEKKNSDIYGPWKKKCLCDFGIVTQCISPTKINDQYI 600

Query: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660
           TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS
Sbjct: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660

Query: 661 RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL 720
           RSWPLISRYRAAVRTQSPK+EMID L+KPLE   DDGIIRELLLDFY TSNGRKP QII+
Sbjct: 661 RSWPLISRYRAAVRTQSPKLEMIDALYKPLEDGNDDGIIRELLLDFYRTSNGRKPAQIIV 720

Query: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP 780
           FRDGVSESQFNQVLNIELDQIVKAYQHLGE NVPKFTVIVGQKNHHTKFFQAGAPENVPP
Sbjct: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEVNVPKFTVIVGQKNHHTKFFQAGAPENVPP 780

Query: 781 GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840
           GTVVDTKVVHP+NYDFYMCAHAGMIGTSRPAHYHVLVDEIGF+PDDLQNLIHSLSYVYQR
Sbjct: 781 GTVVDTKVVHPKNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFAPDDLQNLIHSLSYVYQR 840

Query: 841 STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG 900
           STTALSI APICYAHLAA+QMSQFIKFED SETSSE+G +TS+GS+SI ELPRL K V G
Sbjct: 841 STTALSIAAPICYAHLAAAQMSQFIKFEDHSETSSEQGAITSSGSVSILELPRLHKDVDG 900

Query: 901 SMFFC 905
           SMFFC
Sbjct: 901 SMFFC 904

BLAST of MC04g0083 vs. NCBI nr
Match: XP_038887435.1 (protein argonaute 16-like [Benincasa hispida] >XP_038887436.1 protein argonaute 16-like [Benincasa hispida] >XP_038887437.1 protein argonaute 16-like [Benincasa hispida])

HSP 1 Score: 1646 bits (4262), Expect = 0.0
Identity = 818/906 (90.29%), Postives = 861/906 (95.03%), Query Frame = 0

Query: 1   MVNITNAGGNS-EASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFK 60
           MVNITN  G + EASPLPL P +PP+MK EKA+ P Y+IMSR GVGSKGR+IPLLTNHF 
Sbjct: 1   MVNITNTEGKTNEASPLPLVPSIPPDMKPEKAMSPTYTIMSRCGVGSKGRQIPLLTNHFT 60

Query: 61  VSVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLF 120
           VSV+APDLIFYQY+VSI YED+RPVEGK IGRKLMD++YQTYSAELAN+RFAYDGEK L+
Sbjct: 61  VSVSAPDLIFYQYTVSICYEDSRPVEGKEIGRKLMDKVYQTYSAELANKRFAYDGEKCLY 120

Query: 121 SIGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKI 180
           +IGPLPQNKL FTVVLEGS AKLETG+SGG+GSPNG GKR KRSFQ KTFKVELSFATKI
Sbjct: 121 TIGPLPQNKLVFTVVLEGSCAKLETGTSGGSGSPNGTGKRLKRSFQSKTFKVELSFATKI 180

Query: 181 PMKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGG 240
           P+KSIFTALKGSEVDNGSTQDALRV DIILRQQAA RGCLLVRQSFFHDDSRNF DVGGG
Sbjct: 181 PIKSIFTALKGSEVDNGSTQDALRVFDIILRQQAAKRGCLLVRQSFFHDDSRNFADVGGG 240

Query: 241 VTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRML 300
           VTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDW KAK+ML
Sbjct: 241 VTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWTKAKKML 300

Query: 301 KNLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIEL 360
           KNLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNN STD QMVDITV+EYFVRHCGIEL
Sbjct: 301 KNLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNGSTDGQMVDITVYEYFVRHCGIEL 360

Query: 361 TYSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIV 420
           TYSAYLPCLDVGKPKRP Y+PLELCSLVSLQRYTKALSS+QRASLVEKSRQKPQEKI+I+
Sbjct: 361 TYSAYLPCLDVGKPKRPTYMPLELCSLVSLQRYTKALSSMQRASLVEKSRQKPQEKIKII 420

Query: 421 TDALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKT 480
           TDALKNY YDEDPVLAQC +KID+QLTQ+EGRVLESPKLKVGKSDDCIPRNGRWNFNNKT
Sbjct: 421 TDALKNYGYDEDPVLAQCGVKIDRQLTQVEGRVLESPKLKVGKSDDCIPRNGRWNFNNKT 480

Query: 481 LLNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDR 540
           LLNPTRIDRW+VVNFSARCDTS+ISRELINCGRNKGIHIERP TLIEEDQHSRRASPVDR
Sbjct: 481 LLNPTRIDRWIVVNFSARCDTSYISRELINCGRNKGIHIERPITLIEEDQHSRRASPVDR 540

Query: 541 VENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQY 600
           VENMFEQ++ K+ D P +FILCVLPEKKNSNIYGPWKKKCLCDFGI TQCISPTKINDQY
Sbjct: 541 VENMFEQMMAKMSDAP-TFILCVLPEKKNSNIYGPWKKKCLCDFGIVTQCISPTKINDQY 600

Query: 601 ITNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVG 660
           ITNVLLKINSKLGGINSLLAIEHASCVP+IKDTPTLILGMDVSHGSPGRSDVPSIAAVVG
Sbjct: 601 ITNVLLKINSKLGGINSLLAIEHASCVPMIKDTPTLILGMDVSHGSPGRSDVPSIAAVVG 660

Query: 661 SRSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQII 720
           SRSWPLISRYRAAVRTQSPKVEMID LFKPLE  +DDGIIRELLLDFYSTS GRKPTQI+
Sbjct: 661 SRSWPLISRYRAAVRTQSPKVEMIDALFKPLEDGKDDGIIRELLLDFYSTSKGRKPTQIL 720

Query: 721 LFRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVP 780
           +FRDGVSESQFNQVLN+ELDQIVKAYQHLGE NVPKFTVIV QKNHHTKFFQ GAPENVP
Sbjct: 721 VFRDGVSESQFNQVLNVELDQIVKAYQHLGEVNVPKFTVIVAQKNHHTKFFQPGAPENVP 780

Query: 781 PGTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQ 840
           PGTVVDTKVVHP+NYDFYMCAHAGMIGTSRPAHYHVL+DEIGFSPDDLQNLIHSLSYVYQ
Sbjct: 781 PGTVVDTKVVHPKNYDFYMCAHAGMIGTSRPAHYHVLLDEIGFSPDDLQNLIHSLSYVYQ 840

Query: 841 RSTTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVA 900
           RSTTALSI APICYAHLAASQMSQFIKFE+LSETSSERGT+TS+GSLSIPELPRL + V 
Sbjct: 841 RSTTALSIAAPICYAHLAASQMSQFIKFEELSETSSERGTITSSGSLSIPELPRLHQDVD 900

Query: 901 GSMFFC 905
           GSMFFC
Sbjct: 901 GSMFFC 905

BLAST of MC04g0083 vs. ExPASy TrEMBL
Match: A0A6J1D3Q4 (protein argonaute 16 OS=Momordica charantia OX=3673 GN=LOC111016747 PE=3 SV=1)

HSP 1 Score: 1811 bits (4692), Expect = 0.0
Identity = 905/905 (100.00%), Postives = 905/905 (100.00%), Query Frame = 0

Query: 1   MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV 60
           MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV
Sbjct: 1   MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV 60

Query: 61  SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS 120
           SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS
Sbjct: 61  SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS 120

Query: 121 IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP 180
           IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP
Sbjct: 121 IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP 180

Query: 181 MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV 240
           MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV
Sbjct: 181 MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV 240

Query: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK 300
           TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK
Sbjct: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK 300

Query: 301 NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT 360
           NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT
Sbjct: 301 NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT 360

Query: 361 YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT 420
           YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT
Sbjct: 361 YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT 420

Query: 421 DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL 480
           DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL
Sbjct: 421 DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL 480

Query: 481 LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV 540
           LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV
Sbjct: 481 LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV 540

Query: 541 ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI 600
           ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI
Sbjct: 541 ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI 600

Query: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660
           TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS
Sbjct: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660

Query: 661 RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL 720
           RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL
Sbjct: 661 RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL 720

Query: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP 780
           FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP
Sbjct: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP 780

Query: 781 GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840
           GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR
Sbjct: 781 GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840

Query: 841 STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG 900
           STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG
Sbjct: 841 STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG 900

Query: 901 SMFFC 905
           SMFFC
Sbjct: 901 SMFFC 905

BLAST of MC04g0083 vs. ExPASy TrEMBL
Match: A0A6J1JHU1 (protein argonaute 16 OS=Cucurbita maxima OX=3661 GN=LOC111485919 PE=3 SV=1)

HSP 1 Score: 1651 bits (4275), Expect = 0.0
Identity = 821/905 (90.72%), Postives = 858/905 (94.81%), Query Frame = 0

Query: 1   MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV 60
           MV+IT++ G SEA  LP G P+PP MK EKAVPP Y+IMSRRGVGSKGRRIPLL NHF+V
Sbjct: 1   MVDITHSEGESEAVALPPGTPIPPIMKPEKAVPPTYTIMSRRGVGSKGRRIPLLANHFRV 60

Query: 61  SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS 120
           SVNAPDLIFYQYSVSISYEDN+PVEGK IGRKLMD+LYQTYSAELAN+RFAYDGEK L+S
Sbjct: 61  SVNAPDLIFYQYSVSISYEDNKPVEGKEIGRKLMDKLYQTYSAELANKRFAYDGEKCLYS 120

Query: 121 IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP 180
           IGPLPQNK +FTVVLEGSYAK E+G+S  +GSPNG+GKRSK SFQ KTFKVELSFATKIP
Sbjct: 121 IGPLPQNKFDFTVVLEGSYAKPESGNSRSSGSPNGVGKRSKHSFQSKTFKVELSFATKIP 180

Query: 181 MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV 240
           MKSIFTALKGSEVDNG TQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNF D+GGGV
Sbjct: 181 MKSIFTALKGSEVDNGGTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFTDIGGGV 240

Query: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK 300
           TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPG VIDFLIANQNVREPRYIDW KAKRMLK
Sbjct: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGSVIDFLIANQNVREPRYIDWVKAKRMLK 300

Query: 301 NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT 360
           NLRV ARHRNMEFKI GLSEKPCNQQFFSMKLKNN STD +M+DITV+EYFVRHCGIELT
Sbjct: 301 NLRVNARHRNMEFKITGLSEKPCNQQFFSMKLKNNGSTDGEMIDITVYEYFVRHCGIELT 360

Query: 361 YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT 420
           YSAYLPCLDVGK KRPNYLPLELCSLVSLQRYTKALSS+QRASLVEKSRQKPQEKI+IVT
Sbjct: 361 YSAYLPCLDVGKSKRPNYLPLELCSLVSLQRYTKALSSMQRASLVEKSRQKPQEKIKIVT 420

Query: 421 DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL 480
           DALK+YRYDEDPVLAQC LKIDKQLTQ+EGRVLESPKLKVG+SDDCIPRNGRWNFNNKTL
Sbjct: 421 DALKSYRYDEDPVLAQCGLKIDKQLTQVEGRVLESPKLKVGRSDDCIPRNGRWNFNNKTL 480

Query: 481 LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV 540
           LNPTRID W+VVNFSARCDTS+ISRELINCGRNKGIHIERP TLIEEDQHSRRASPVDRV
Sbjct: 481 LNPTRIDSWIVVNFSARCDTSYISRELINCGRNKGIHIERPITLIEEDQHSRRASPVDRV 540

Query: 541 ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI 600
           ENMFEQI+ KLP+ PPSFILCVLPEKKNSNIYGPWKKKCLCDFGI TQCISPTKINDQYI
Sbjct: 541 ENMFEQIMAKLPN-PPSFILCVLPEKKNSNIYGPWKKKCLCDFGIVTQCISPTKINDQYI 600

Query: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660
           TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS
Sbjct: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660

Query: 661 RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL 720
           RSWPLISRYRAAVRTQSPK+EMID L+KPLE   DDGIIRELLLDFY TSNGRKP QII+
Sbjct: 661 RSWPLISRYRAAVRTQSPKLEMIDALYKPLEDGNDDGIIRELLLDFYRTSNGRKPAQIIV 720

Query: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP 780
           FRDGVSESQFNQVLNIELDQIVKAYQHLGE NVPKFTVIVGQKNHHTKFFQAGAPENVPP
Sbjct: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEVNVPKFTVIVGQKNHHTKFFQAGAPENVPP 780

Query: 781 GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840
           GTVVDTKVVHP+NYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR
Sbjct: 781 GTVVDTKVVHPKNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840

Query: 841 STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG 900
           STTALSI APICYAHLAA+QMSQFIKFED SETSSE+G +TS+GS+SI ELPRL K V G
Sbjct: 841 STTALSIAAPICYAHLAAAQMSQFIKFEDHSETSSEQGAITSSGSVSILELPRLHKDVDG 900

Query: 901 SMFFC 905
           SMFFC
Sbjct: 901 SMFFC 904

BLAST of MC04g0083 vs. ExPASy TrEMBL
Match: A0A6J1ERU4 (protein argonaute 16-like OS=Cucurbita moschata OX=3662 GN=LOC111437218 PE=3 SV=1)

HSP 1 Score: 1648 bits (4267), Expect = 0.0
Identity = 822/905 (90.83%), Postives = 856/905 (94.59%), Query Frame = 0

Query: 1   MVNITNAGGNSEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKV 60
           MV+IT++ G SEA  LP   PVPP MK EKAVPP Y+IMSRRGVGSKGRRIPLLTNHF+V
Sbjct: 1   MVDITHSEGESEAVALPPDTPVPPIMKPEKAVPPTYTIMSRRGVGSKGRRIPLLTNHFRV 60

Query: 61  SVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFS 120
           SVNAPDLIFYQYSVSISYEDN+PVEGK IGRKLMD+LYQTYSAELAN+RFAYDGEK L+S
Sbjct: 61  SVNAPDLIFYQYSVSISYEDNKPVEGKEIGRKLMDKLYQTYSAELANKRFAYDGEKCLYS 120

Query: 121 IGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIP 180
           IGPLPQNK +FTVVLEGSYAK E+G+S  +GSPNG GKRSK SFQ KTFKVELSFATKIP
Sbjct: 121 IGPLPQNKFDFTVVLEGSYAKPESGNSRSSGSPNGAGKRSKHSFQSKTFKVELSFATKIP 180

Query: 181 MKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGV 240
           MKSIFTALKGSEVDNG TQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNF D+GGGV
Sbjct: 181 MKSIFTALKGSEVDNGGTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFTDIGGGV 240

Query: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLK 300
           TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPG VIDFLIANQNVREPRYIDW KAKRMLK
Sbjct: 241 TGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGSVIDFLIANQNVREPRYIDWVKAKRMLK 300

Query: 301 NLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELT 360
           NLRV ARHRNMEFKI GLSEKPCNQQFFSMKLKNN STD +M+DITV+EYFVRHCGIELT
Sbjct: 301 NLRVNARHRNMEFKITGLSEKPCNQQFFSMKLKNNGSTDGEMIDITVYEYFVRHCGIELT 360

Query: 361 YSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVT 420
           YSAYLPCLDVGK KRPNYLPLELCSLVSLQRYTKALSS+QRASLVEKSRQKPQEKI+IVT
Sbjct: 361 YSAYLPCLDVGKSKRPNYLPLELCSLVSLQRYTKALSSMQRASLVEKSRQKPQEKIKIVT 420

Query: 421 DALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTL 480
           DALK+YRYDEDPVLAQC LKIDKQLTQ+EGRVLESPKLKVG+SDDCIPRNGRWNFNNKTL
Sbjct: 421 DALKSYRYDEDPVLAQCGLKIDKQLTQVEGRVLESPKLKVGRSDDCIPRNGRWNFNNKTL 480

Query: 481 LNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRV 540
           LNPTRID W+VVNFSARCDTS+ISRELINCGRNKGIHIERP TLIEEDQHSRRASPVDRV
Sbjct: 481 LNPTRIDSWIVVNFSARCDTSYISRELINCGRNKGIHIERPITLIEEDQHSRRASPVDRV 540

Query: 541 ENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYI 600
           ENMFEQI+ KLP+ PPSFILCVLPEKKNSNIYGPWKKKCLCDFGI TQCISPTKINDQYI
Sbjct: 541 ENMFEQIMAKLPN-PPSFILCVLPEKKNSNIYGPWKKKCLCDFGIVTQCISPTKINDQYI 600

Query: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660
           TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS
Sbjct: 601 TNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGS 660

Query: 661 RSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIIL 720
           RSWPLISRYRAAVRTQSPK+EMID L+KPLE   DDGIIRELLLDFY TSNGRKP QII+
Sbjct: 661 RSWPLISRYRAAVRTQSPKLEMIDALYKPLEDGNDDGIIRELLLDFYRTSNGRKPAQIIV 720

Query: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPP 780
           FRDGVSESQFNQVLNIELDQIVKAYQHLGE NVPKFTVIVGQKNHHTKFFQAGAPENVPP
Sbjct: 721 FRDGVSESQFNQVLNIELDQIVKAYQHLGEVNVPKFTVIVGQKNHHTKFFQAGAPENVPP 780

Query: 781 GTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840
           GTVVDTKVVHP+NYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR
Sbjct: 781 GTVVDTKVVHPKNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQR 840

Query: 841 STTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAG 900
           STTALSI APICYAHLAA+QMSQFIKFED SETSSE G +TS+GS+SI ELPRL K V G
Sbjct: 841 STTALSIAAPICYAHLAAAQMSQFIKFEDHSETSSELGAITSSGSVSILELPRLHKDVDG 900

Query: 901 SMFFC 905
           SMFFC
Sbjct: 901 SMFFC 904

BLAST of MC04g0083 vs. ExPASy TrEMBL
Match: A0A6J1GJG1 (LOW QUALITY PROTEIN: protein argonaute 16-like OS=Cucurbita moschata OX=3662 GN=LOC111454846 PE=3 SV=1)

HSP 1 Score: 1641 bits (4249), Expect = 0.0
Identity = 819/906 (90.40%), Postives = 851/906 (93.93%), Query Frame = 0

Query: 1   MVNITNAGGN-SEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFK 60
           M+NITN  G  SEASPLPL PPVPP+ K EK +PP Y+IMSRRGVGSKGRRIPLLTNHF 
Sbjct: 1   MINITNIQGKRSEASPLPLTPPVPPDTKPEKTMPPTYTIMSRRGVGSKGRRIPLLTNHFG 60

Query: 61  VSVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLF 120
           VSVNAPDLIFYQYSVSI YEDNRPVEGK IGRKLMD+LYQTYS ELAN+RFAYDGEK L+
Sbjct: 61  VSVNAPDLIFYQYSVSICYEDNRPVEGKEIGRKLMDKLYQTYSTELANKRFAYDGEKCLY 120

Query: 121 SIGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKI 180
            +GPLPQNKLEFTVVLEGSYAKLETG SGG+GSPNGIGKRSK SFQ KTFKVELSFATKI
Sbjct: 121 CVGPLPQNKLEFTVVLEGSYAKLETGRSGGSGSPNGIGKRSKCSFQSKTFKVELSFATKI 180

Query: 181 PMKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGG 240
           PMKSIFTALKG EVDNG +QDALRVLDIILRQQAAN+GCLLVRQSFFHDDSRNF DVGGG
Sbjct: 181 PMKSIFTALKGEEVDNGHSQDALRVLDIILRQQAANQGCLLVRQSFFHDDSRNFADVGGG 240

Query: 241 VTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRML 300
           VTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDW KAKRML
Sbjct: 241 VTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWVKAKRML 300

Query: 301 KNLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIEL 360
           KNLRVKA HRNME KIIGLSEKPCNQQFFSMKLKNN S D QMVD+TV+EYFVRHCGIEL
Sbjct: 301 KNLRVKASHRNMESKIIGLSEKPCNQQFFSMKLKNNGSADGQMVDVTVYEYFVRHCGIEL 360

Query: 361 TYSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIV 420
           TYSAYLPCLDVGKPKRP  LPLELCSLVSLQRYTKALS +QRASLVEKSRQKPQEKI+++
Sbjct: 361 TYSAYLPCLDVGKPKRPAXLPLELCSLVSLQRYTKALSLMQRASLVEKSRQKPQEKIKVL 420

Query: 421 TDALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKT 480
           TDALKNY+YDEDPVLAQC LKID+Q TQ+EGRVLESPKL+VGKSDDCIPRNGRWNFNNKT
Sbjct: 421 TDALKNYQYDEDPVLAQCGLKIDRQPTQVEGRVLESPKLEVGKSDDCIPRNGRWNFNNKT 480

Query: 481 LLNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDR 540
           LLNPTRIDRW+VVNFSARCD S+ISRELINCG NKGIHIERP TLIEEDQHSRRASPVDR
Sbjct: 481 LLNPTRIDRWIVVNFSARCDXSYISRELINCGGNKGIHIERPITLIEEDQHSRRASPVDR 540

Query: 541 VENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQY 600
           VENMFEQ + K+ D P SFILCVLPEKKNSNIYGPWKKKCLCDFGI TQCISPTKINDQY
Sbjct: 541 VENMFEQTVGKVSDAP-SFILCVLPEKKNSNIYGPWKKKCLCDFGIVTQCISPTKINDQY 600

Query: 601 ITNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVG 660
           ITNVLLKIN KLGGINSLLAIEHA CVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVG
Sbjct: 601 ITNVLLKINYKLGGINSLLAIEHAPCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVG 660

Query: 661 SRSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQII 720
           SRSWPLISRYRAAVRTQSPKVEMID L+KPLE   DDGIIRELLLDFY TSNGRKPTQII
Sbjct: 661 SRSWPLISRYRAAVRTQSPKVEMIDALYKPLEDGNDDGIIRELLLDFYRTSNGRKPTQII 720

Query: 721 LFRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVP 780
           +FRDGVSESQFNQVLNIELDQIVKAYQHLGE NVPKFTVIVGQKNHHTKFFQ GAPENVP
Sbjct: 721 VFRDGVSESQFNQVLNIELDQIVKAYQHLGEVNVPKFTVIVGQKNHHTKFFQPGAPENVP 780

Query: 781 PGTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQ 840
           PGTVVDTKVVHP+NYDFYMCAHAGMIGTSRPAHYHVL+DEIGFSPDDLQNLIHSLSYVYQ
Sbjct: 781 PGTVVDTKVVHPKNYDFYMCAHAGMIGTSRPAHYHVLLDEIGFSPDDLQNLIHSLSYVYQ 840

Query: 841 RSTTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVA 900
           RSTTALSI APICYAHLAASQMSQFI FE+LSETSSERGT+TS+GSLSIPELPRL K V 
Sbjct: 841 RSTTALSIAAPICYAHLAASQMSQFINFEELSETSSERGTITSSGSLSIPELPRLHKDVN 900

Query: 901 GSMFFC 905
           GSMFFC
Sbjct: 901 GSMFFC 905

BLAST of MC04g0083 vs. ExPASy TrEMBL
Match: A0A5A7V595 (Protein argonaute 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold212G00250 PE=3 SV=1)

HSP 1 Score: 1625 bits (4207), Expect = 0.0
Identity = 811/906 (89.51%), Postives = 854/906 (94.26%), Query Frame = 0

Query: 1   MVNITNAGGN-SEASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFK 60
           MVNITN  G  SE SPL L P +PP+MK EKA+P  Y IMSRRGVGSKGRRIPLLTNHF+
Sbjct: 1   MVNITNTEGKTSETSPLGLAPSIPPDMKPEKAMP-LYKIMSRRGVGSKGRRIPLLTNHFR 60

Query: 61  VSVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLF 120
           VS+NAPDL+FYQY+VSI YED+RPVEGK IGRKLMD+LYQT+S ELAN+RFAYDGEK L+
Sbjct: 61  VSLNAPDLVFYQYAVSICYEDSRPVEGKEIGRKLMDKLYQTHSTELANKRFAYDGEKCLY 120

Query: 121 SIGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKI 180
           +IGPLPQ KLEF+VVLEG  AK+ETGSSG +GSPNG GKR KRS Q KTFK+ELSFATKI
Sbjct: 121 TIGPLPQRKLEFSVVLEGFCAKIETGSSGESGSPNGTGKRFKRSSQSKTFKIELSFATKI 180

Query: 181 PMKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGG 240
           PMKSIFTALKGSE DNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNF DVGGG
Sbjct: 181 PMKSIFTALKGSEEDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFADVGGG 240

Query: 241 VTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRML 300
           VTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDW KAK+ML
Sbjct: 241 VTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWGKAKKML 300

Query: 301 KNLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIEL 360
           KNLRV+ARHRNMEFKIIGLSEKPCNQQFFSMKLKNN STD QMVDITV+EYFVRHCGIEL
Sbjct: 301 KNLRVRARHRNMEFKIIGLSEKPCNQQFFSMKLKNNGSTDGQMVDITVYEYFVRHCGIEL 360

Query: 361 TYSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIV 420
           TYSAYLPCLDVGKPKRP Y+PLELCSLVSLQRYTKALSS+QRASLVEKSRQKPQEKI+IV
Sbjct: 361 TYSAYLPCLDVGKPKRPTYIPLELCSLVSLQRYTKALSSMQRASLVEKSRQKPQEKIKIV 420

Query: 421 TDALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKT 480
           TDALKNYRYDEDPVLAQC +KID+QLTQ+EGRVLESPKLKVGKSDDCIPRNGRWNFNNKT
Sbjct: 421 TDALKNYRYDEDPVLAQCGVKIDRQLTQVEGRVLESPKLKVGKSDDCIPRNGRWNFNNKT 480

Query: 481 LLNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDR 540
           LLNPTRI+RW+VVNFSARCD S+ISRELINCGRNKGIHIERP TLIEEDQHSRRASPVDR
Sbjct: 481 LLNPTRINRWIVVNFSARCDISYISRELINCGRNKGIHIERPITLIEEDQHSRRASPVDR 540

Query: 541 VENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQY 600
           VENMFEQ++ K+ D P +FILCVLPEKKNSNIYGPWKKKCLCDFGI TQCISPTKINDQY
Sbjct: 541 VENMFEQMMAKMSDAP-TFILCVLPEKKNSNIYGPWKKKCLCDFGIFTQCISPTKINDQY 600

Query: 601 ITNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVG 660
           ITNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVG
Sbjct: 601 ITNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVG 660

Query: 661 SRSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQII 720
           SRSWPLISRYRAAVRTQSPK+EMID LFKPLEG +DDGIIRELLLDFYSTS GRKPTQII
Sbjct: 661 SRSWPLISRYRAAVRTQSPKLEMIDALFKPLEGGKDDGIIRELLLDFYSTSKGRKPTQII 720

Query: 721 LFRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVP 780
           +FRDGVSESQFNQVLNIELDQIVKAYQHLGE N+PKFTVIV QKNHHT+FF  GA ENVP
Sbjct: 721 VFRDGVSESQFNQVLNIELDQIVKAYQHLGEVNIPKFTVIVAQKNHHTRFFLPGASENVP 780

Query: 781 PGTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQ 840
           PGTVVDT+VVHP+NYDFYMCAHAGMIGTSRPAHYHVL+DEIGFSPDDLQNLIHSLSYVYQ
Sbjct: 781 PGTVVDTRVVHPKNYDFYMCAHAGMIGTSRPAHYHVLLDEIGFSPDDLQNLIHSLSYVYQ 840

Query: 841 RSTTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVA 900
           RSTTALSI APICYAHLAASQMSQFIKFE+LSETSSERG VTS+GSLSIPELPRL   V 
Sbjct: 841 RSTTALSIAAPICYAHLAASQMSQFIKFEELSETSSERGGVTSSGSLSIPELPRLHDDVN 900

Query: 901 GSMFFC 905
           GSMFFC
Sbjct: 901 GSMFFC 904

BLAST of MC04g0083 vs. TAIR 10
Match: AT2G32940.1 (Argonaute family protein )

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 572/895 (63.91%), Postives = 692/895 (77.32%), Query Frame = 0

Query: 13  ASPLPLGPPVPPNMKLEKAVPPKYSIMSRRGVGSKGRRIPLLTNHFKVSVNAPDLIFYQY 72
           +S LPL    P +++ E+     Y I +RRGVG+ G  I L TNHF VSV  PD++FYQY
Sbjct: 4   SSSLPLS---PISIEPEQPSHRDYDITTRRGVGTTGNPIELCTNHFNVSVRQPDVVFYQY 63

Query: 73  SVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFSIGPLPQNKLEFT 132
           +VSI+ E+   V+G GI RKLMDQL++TYS++L  +R AYDGEKTL+++GPLPQN+ +F 
Sbjct: 64  TVSITTENGDAVDGTGISRKLMDQLFKTYSSDLDGKRLAYDGEKTLYTVGPLPQNEFDFL 123

Query: 133 VVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIPMKSIFTALKGSE 192
           V++EGS++K + G S G GS +G  KRSKRSF  +++KV++ +A +IP+K++    +G+ 
Sbjct: 124 VIVEGSFSKRDCGVSDG-GSSSGTCKRSKRSFLPRSYKVQIHYAAEIPLKTVLGTQRGAY 183

Query: 193 VDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGVTGVRGFHSSFRL 252
             + S QDALRVLDI+LRQQAA RGCLLVRQ+FFH D    + VGGGV G+RG HSSFR 
Sbjct: 184 TPDKSAQDALRVLDIVLRQQAAERGCLLVRQAFFHSDGHP-MKVGGGVIGIRGLHSSFRP 243

Query: 253 AQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLKNLRVKARHRNME 312
              GLSLN+DVSTTMIL+PGPVI+FL ANQ+V  PR IDW KA +MLK++RVKA HRNME
Sbjct: 244 THGGLSLNIDVSTTMILEPGPVIEFLKANQSVETPRQIDWIKAAKMLKHMRVKATHRNME 303

Query: 313 FKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELTYSAYLPCLDVGK 372
           FKIIGLS KPCNQQ FSMK+K +   +  + +ITV++YF +    E   SAY PCLDVGK
Sbjct: 304 FKIIGLSSKPCNQQLFSMKIK-DGEREVPIREITVYDYF-KQTYTEPISSAYFPCLDVGK 363

Query: 373 PKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVTDALKNYRYDEDP 432
           P RPNYLPLE C+LVSLQRYTK LS  QR  LVE SRQKP E+I+ + DA+  Y YD+DP
Sbjct: 364 PDRPNYLPLEFCNLVSLQRYTKPLSGRQRVLLVESSRQKPLERIKTLNDAMHTYCYDKDP 423

Query: 433 VLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTLLNPTRIDRWVVV 492
            LA C + I+K++TQ+EGRVL+ P LK GK++D  P NGRWNFNNK LL P  I  W +V
Sbjct: 424 FLAGCGISIEKEMTQVEGRVLKPPMLKFGKNEDFQPCNGRWNFNNKMLLEPRAIKSWAIV 483

Query: 493 NFSARCDTSFISRELINCGRNKGIHIERPCTLIEEDQHSRRASPVDRVENMFEQIITKLP 552
           NFS  CD+S ISRELI+CG  KGI I+RP  L+EED   ++A PV+RVE M   +  K P
Sbjct: 484 NFSFPCDSSHISRELISCGMRKGIEIDRPFALVEEDPQYKKAGPVERVEKMIATMKLKFP 543

Query: 553 DKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYITNVLLKINSKLG 612
           D PP FILC+LPE+K S+IYGPWKK CL + GI TQCI P KI+DQY+TNVLLKINSKLG
Sbjct: 544 D-PPHFILCILPERKTSDIYGPWKKICLTEEGIHTQCICPIKISDQYLTNVLLKINSKLG 603

Query: 613 GINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGSRSWPLISRYRAA 672
           GINSLL IE++  +PLI   PTLILGMDVSHG PGR+DVPS+AAVVGS+ WPLISRYRAA
Sbjct: 604 GINSLLGIEYSYNIPLINKIPTLILGMDVSHGPPGRADVPSVAAVVGSKCWPLISRYRAA 663

Query: 673 VRTQSPKVEMIDGLFKPLEGDQ--DDGIIRELLLDFYSTSNGRKPTQIILFRDGVSESQF 732
           VRTQSP++EMID LF+P+E  +  D+GI+ EL ++FY TS  RKP QII+FRDGVSESQF
Sbjct: 664 VRTQSPRLEMIDSLFQPIENTEKGDNGIMNELFVEFYRTSRARKPKQIIIFRDGVSESQF 723

Query: 733 NQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPPGTVVDTKVVH 792
            QVL IE+DQI+KAYQ LGE++VPKFTVIV QKNHHTK FQA  PENVP GTVVDTK+VH
Sbjct: 724 EQVLKIEVDQIIKAYQRLGESDVPKFTVIVAQKNHHTKLFQAKGPENVPAGTVVDTKIVH 783

Query: 793 PRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQRSTTALSIVAP 852
           P NYDFYMCAHAG IGTSRPAHYHVL+DEIGFSPDDLQNLIHSLSYV QRSTTA SIVAP
Sbjct: 784 PTNYDFYMCAHAGKIGTSRPAHYHVLLDEIGFSPDDLQNLIHSLSYVNQRSTTATSIVAP 843

Query: 853 ICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAGSMFFC 906
           + YAHLAA+Q++QF KFE +SE               +PELPRL + V G+MFFC
Sbjct: 844 VRYAHLAAAQVAQFTKFEGISEDG------------KVPELPRLHENVEGNMFFC 878

BLAST of MC04g0083 vs. TAIR 10
Match: AT2G27040.1 (Argonaute family protein )

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 544/919 (59.19%), Postives = 691/919 (75.19%), Query Frame = 0

Query: 3   NITNAGGNSEASPLPLGPPVPPNM-------KLEKAVPPKYSIMSRRGVGSKGRRIPLLT 62
           N  N  G +EA P P  P +PPN+       +L +   P    M+R+G G++G++IPLLT
Sbjct: 15  NGANGSGVTEALP-PPPPVIPPNVEPVRVKTELAEKKGPVRVPMARKGFGTRGQKIPLLT 74

Query: 63  NHFKVSVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGE 122
           NHFKV V      F+ YSV++ Y+D RPVE KG+GRK++D+++QTY ++L  + FAYDGE
Sbjct: 75  NHFKVDVANLQGHFFHYSVALFYDDGRPVEQKGVGRKILDKVHQTYHSDLDGKEFAYDGE 134

Query: 123 KTLFSIGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPN-------GIGKRSKRSFQLKT 182
           KTLF+ G LP NK++F+VVLE    ++    + GNGSPN       G  KR +R  + K 
Sbjct: 135 KTLFTYGALPSNKMDFSVVLE----EVSATRANGNGSPNGNESPSDGDRKRLRRPNRSKN 194

Query: 183 FKVELSFATKIPMKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHD 242
           F+VE+S+A KIP++++  A++G E +N  +Q+A+RVLDIILRQ AA +GCLLVRQSFFH+
Sbjct: 195 FRVEISYAAKIPLQALANAMRGQESEN--SQEAIRVLDIILRQHAARQGCLLVRQSFFHN 254

Query: 243 DSRNFVDVGGGVTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPR 302
           D  N   VGG + G RGFHSSFR  Q G+SLNMDV+TTMI+KPGPV+DFLIANQN R+P 
Sbjct: 255 DPTNCEPVGGNILGCRGFHSSFRTTQGGMSLNMDVTTTMIIKPGPVVDFLIANQNARDPY 314

Query: 303 YIDWAKAKRMLKNLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKN-NSSTDEQMVDITV 362
            IDW+KAKR LKNLRVK      EFKI GLS+KPC +Q F +K +N N + + +  ++TV
Sbjct: 315 SIDWSKAKRTLKNLRVKVSPSGQEFKITGLSDKPCREQTFELKKRNPNENGEFETTEVTV 374

Query: 363 HEYFVRHCGIELTYSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEK 422
            +YF     I+L YSA LPC++VGKPKRP Y+PLELC+LV LQRYTKAL++ QR++LVEK
Sbjct: 375 ADYFRDTRHIDLQYSADLPCINVGKPKRPTYIPLELCALVPLQRYTKALTTFQRSALVEK 434

Query: 423 SRQKPQEKIRIVTDALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCI 482
           SRQKPQE++ +++ ALK   YD +P+L  C + I    TQ+EGRVL +PKLK+G   +  
Sbjct: 435 SRQKPQERMTVLSKALKVSNYDAEPLLRSCGISISSNFTQVEGRVLPAPKLKMGCGSETF 494

Query: 483 PRNGRWNFNNKTLLNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEE 542
           PRNGRWNFNNK  + PT+I RWVVVNFSARC+   +  +LI  G +KGI I  P  + EE
Sbjct: 495 PRNGRWNFNNKEFVEPTKIQRWVVVNFSARCNVRQVVDDLIKIGGSKGIEIASPFQVFEE 554

Query: 543 DQHSRRASPVDRVENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIAT 602
               RRA P+ RVENMF+ I +KLP   P FILCVLP+KKNS++YGPWKKK L +FGI T
Sbjct: 555 GNQFRRAPPMIRVENMFKDIQSKLPG-VPQFILCVLPDKKNSDLYGPWKKKNLTEFGIVT 614

Query: 603 QCISPTK-INDQYITNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSP 662
           QC++PT+  NDQY+TN+LLKIN+KLGG+NS+L++E      +I   PT+ILGMDVSHGSP
Sbjct: 615 QCMAPTRQPNDQYLTNLLLKINAKLGGLNSMLSVERTPAFTVISKVPTIILGMDVSHGSP 674

Query: 663 GRSDVPSIAAVVGSRSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDF 722
           G+SDVPSIAAVV SR WPLIS+YRA+VRTQ  K EMI+ L K   G +DDGII+ELL+DF
Sbjct: 675 GQSDVPSIAAVVSSREWPLISKYRASVRTQPSKAEMIESLVKK-NGTEDDGIIKELLVDF 734

Query: 723 YSTSNGRKPTQIILFRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHH 782
           Y++SN RKP  II+FRDGVSESQFNQVLNIELDQI++A + L     PKF ++V QKNHH
Sbjct: 735 YTSSNKRKPEHIIIFRDGVSESQFNQVLNIELDQIIEACKLLDANWNPKFLLLVAQKNHH 794

Query: 783 TKFFQAGAPENVPPGTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDD 842
           TKFFQ  +PENVPPGT++D K+ HP+N DFY+CAHAGMIGT+RP HYHVL DEIGFS D+
Sbjct: 795 TKFFQPTSPENVPPGTIIDNKICHPKNNDFYLCAHAGMIGTTRPTHYHVLYDEIGFSADE 854

Query: 843 LQNLIHSLSYVYQRSTTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSL 902
           LQ L+HSLSYVYQRST+A+S+VAPICYAHLAA+Q+  F+KFED SETSS  G +T+ G +
Sbjct: 855 LQELVHSLSYVYQRSTSAISVVAPICYAHLAAAQLGTFMKFEDQSETSSSHGGITAPGPI 914

Query: 903 SIPELPRLDKKVAGSMFFC 906
           S+ +LPRL   VA SMFFC
Sbjct: 915 SVAQLPRLKDNVANSMFFC 924

BLAST of MC04g0083 vs. TAIR 10
Match: AT2G27040.2 (Argonaute family protein )

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 544/919 (59.19%), Postives = 691/919 (75.19%), Query Frame = 0

Query: 3   NITNAGGNSEASPLPLGPPVPPNM-------KLEKAVPPKYSIMSRRGVGSKGRRIPLLT 62
           N  N  G +EA P P  P +PPN+       +L +   P    M+R+G G++G++IPLLT
Sbjct: 15  NGANGSGVTEALP-PPPPVIPPNVEPVRVKTELAEKKGPVRVPMARKGFGTRGQKIPLLT 74

Query: 63  NHFKVSVNAPDLIFYQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGE 122
           NHFKV V      F+ YSV++ Y+D RPVE KG+GRK++D+++QTY ++L  + FAYDGE
Sbjct: 75  NHFKVDVANLQGHFFHYSVALFYDDGRPVEQKGVGRKILDKVHQTYHSDLDGKEFAYDGE 134

Query: 123 KTLFSIGPLPQNKLEFTVVLEGSYAKLETGSSGGNGSPN-------GIGKRSKRSFQLKT 182
           KTLF+ G LP NK++F+VVLE    ++    + GNGSPN       G  KR +R  + K 
Sbjct: 135 KTLFTYGALPSNKMDFSVVLE----EVSATRANGNGSPNGNESPSDGDRKRLRRPNRSKN 194

Query: 183 FKVELSFATKIPMKSIFTALKGSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHD 242
           F+VE+S+A KIP++++  A++G E +N  +Q+A+RVLDIILRQ AA +GCLLVRQSFFH+
Sbjct: 195 FRVEISYAAKIPLQALANAMRGQESEN--SQEAIRVLDIILRQHAARQGCLLVRQSFFHN 254

Query: 243 DSRNFVDVGGGVTGVRGFHSSFRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPR 302
           D  N   VGG + G RGFHSSFR  Q G+SLNMDV+TTMI+KPGPV+DFLIANQN R+P 
Sbjct: 255 DPTNCEPVGGNILGCRGFHSSFRTTQGGMSLNMDVTTTMIIKPGPVVDFLIANQNARDPY 314

Query: 303 YIDWAKAKRMLKNLRVKARHRNMEFKIIGLSEKPCNQQFFSMKLKN-NSSTDEQMVDITV 362
            IDW+KAKR LKNLRVK      EFKI GLS+KPC +Q F +K +N N + + +  ++TV
Sbjct: 315 SIDWSKAKRTLKNLRVKVSPSGQEFKITGLSDKPCREQTFELKKRNPNENGEFETTEVTV 374

Query: 363 HEYFVRHCGIELTYSAYLPCLDVGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEK 422
            +YF     I+L YSA LPC++VGKPKRP Y+PLELC+LV LQRYTKAL++ QR++LVEK
Sbjct: 375 ADYFRDTRHIDLQYSADLPCINVGKPKRPTYIPLELCALVPLQRYTKALTTFQRSALVEK 434

Query: 423 SRQKPQEKIRIVTDALKNYRYDEDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCI 482
           SRQKPQE++ +++ ALK   YD +P+L  C + I    TQ+EGRVL +PKLK+G   +  
Sbjct: 435 SRQKPQERMTVLSKALKVSNYDAEPLLRSCGISISSNFTQVEGRVLPAPKLKMGCGSETF 494

Query: 483 PRNGRWNFNNKTLLNPTRIDRWVVVNFSARCDTSFISRELINCGRNKGIHIERPCTLIEE 542
           PRNGRWNFNNK  + PT+I RWVVVNFSARC+   +  +LI  G +KGI I  P  + EE
Sbjct: 495 PRNGRWNFNNKEFVEPTKIQRWVVVNFSARCNVRQVVDDLIKIGGSKGIEIASPFQVFEE 554

Query: 543 DQHSRRASPVDRVENMFEQIITKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIAT 602
               RRA P+ RVENMF+ I +KLP   P FILCVLP+KKNS++YGPWKKK L +FGI T
Sbjct: 555 GNQFRRAPPMIRVENMFKDIQSKLPG-VPQFILCVLPDKKNSDLYGPWKKKNLTEFGIVT 614

Query: 603 QCISPTK-INDQYITNVLLKINSKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSP 662
           QC++PT+  NDQY+TN+LLKIN+KLGG+NS+L++E      +I   PT+ILGMDVSHGSP
Sbjct: 615 QCMAPTRQPNDQYLTNLLLKINAKLGGLNSMLSVERTPAFTVISKVPTIILGMDVSHGSP 674

Query: 663 GRSDVPSIAAVVGSRSWPLISRYRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDF 722
           G+SDVPSIAAVV SR WPLIS+YRA+VRTQ  K EMI+ L K   G +DDGII+ELL+DF
Sbjct: 675 GQSDVPSIAAVVSSREWPLISKYRASVRTQPSKAEMIESLVKK-NGTEDDGIIKELLVDF 734

Query: 723 YSTSNGRKPTQIILFRDGVSESQFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHH 782
           Y++SN RKP  II+FRDGVSESQFNQVLNIELDQI++A + L     PKF ++V QKNHH
Sbjct: 735 YTSSNKRKPEHIIIFRDGVSESQFNQVLNIELDQIIEACKLLDANWNPKFLLLVAQKNHH 794

Query: 783 TKFFQAGAPENVPPGTVVDTKVVHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDD 842
           TKFFQ  +PENVPPGT++D K+ HP+N DFY+CAHAGMIGT+RP HYHVL DEIGFS D+
Sbjct: 795 TKFFQPTSPENVPPGTIIDNKICHPKNNDFYLCAHAGMIGTTRPTHYHVLYDEIGFSADE 854

Query: 843 LQNLIHSLSYVYQRSTTALSIVAPICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSL 902
           LQ L+HSLSYVYQRST+A+S+VAPICYAHLAA+Q+  F+KFED SETSS  G +T+ G +
Sbjct: 855 LQELVHSLSYVYQRSTSAISVVAPICYAHLAAAQLGTFMKFEDQSETSSSHGGITAPGPI 914

Query: 903 SIPELPRLDKKVAGSMFFC 906
           S+ +LPRL   VA SMFFC
Sbjct: 915 SVAQLPRLKDNVANSMFFC 924

BLAST of MC04g0083 vs. TAIR 10
Match: AT5G21150.1 (Argonaute family protein )

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 539/897 (60.09%), Postives = 687/897 (76.59%), Query Frame = 0

Query: 14  SPLPLGPPVPPNMKLEKAVPPKYSI---MSR-RGVGSKGRRIPLLTNHFKVSVNAPDLIF 73
           S LP  PP  P   + +  P K +I   M+R RG GSKG++IPLLTNHF V  N P   F
Sbjct: 9   SGLPPPPPFVPANLVPEVEPVKKNILLPMARPRGSGSKGQKIPLLTNHFGVKFNKPSGYF 68

Query: 74  YQYSVSISYEDNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFSIGPLPQNKL 133
           + YSV+I+YED RPVE KGIGRK++D++ +TY ++L  + FAYDGEKTLF++G LP NKL
Sbjct: 69  FHYSVAINYEDGRPVEAKGIGRKILDKVQETYQSDLGAKYFAYDGEKTLFTVGALPSNKL 128

Query: 134 EFTVVLEGSYAKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFATKIPMKSIFTALK 193
           +F+VVLE   +   + +  GN + +   KRS+R  Q K F VE+S+A KIPM++I +AL+
Sbjct: 129 DFSVVLEEIPS---SRNHAGNDTNDADRKRSRRPNQTKKFMVEISYAAKIPMQAIASALQ 188

Query: 194 GSEVDNGSTQDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGVTGVRGFHSS 253
           G E +N   QDALRVLDIILRQ AA +GCLLVRQSFFH+D +NFV +GGGV+G RGFHSS
Sbjct: 189 GKETEN--LQDALRVLDIILRQSAARQGCLLVRQSFFHNDVKNFVPIGGGVSGCRGFHSS 248

Query: 254 FRLAQDGLSLNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLKNLRVKARHR 313
           FR  Q GLSLN+D STTMI++PGPV+DFL+ANQN ++P  +DW KA+R+LKNLRV+    
Sbjct: 249 FRTTQGGLSLNIDTSTTMIVQPGPVVDFLLANQNKKDPYGMDWNKARRVLKNLRVQITLS 308

Query: 314 NMEFKIIGLSEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELTYSAYLPCLD 373
           N E+KI GLSE  C  Q F+ +  N+    E+ V+ITV  Y+ +   IE+ YS   PC++
Sbjct: 309 NREYKISGLSEHSCKDQLFTWRKPNDKGEFEE-VEITVLNYY-KERNIEVRYSGDFPCIN 368

Query: 374 VGKPKRPNYLPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVTDALKNYRYD 433
           VGKPKRP Y P+E C+LVSLQRYTK+L++ QRA+LVEKSRQKP E++  +T  LK+  Y+
Sbjct: 369 VGKPKRPTYFPIEFCNLVSLQRYTKSLTNFQRAALVEKSRQKPPERMASLTKGLKDSNYN 428

Query: 434 EDPVLAQCVLKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTLLNPTRIDRW 493
            DPVL    + I    TQ+EGR+L +P LKVGK ++  P  G+WNF  KTL  PT + RW
Sbjct: 429 ADPVLQDSGVSIITNFTQVEGRILPTPMLKVGKGENLSPIKGKWNFMRKTLAEPTTVTRW 488

Query: 494 VVVNFSARCDTSFISRELINCGRNKGIHIERPC-TLIEEDQHSRRASPVDRVENMFEQII 553
            VVNFSARCDT+ + R+LI CGR KGI++E P   +I E+   R A    RVENMFEQI 
Sbjct: 489 AVVNFSARCDTNTLIRDLIKCGREKGINVEPPFKDVINENPQFRNAPATVRVENMFEQIK 548

Query: 554 TKLPDKPPSFILCVLPEKKNSNIYGPWKKKCLCDFGIATQCISPTKINDQYITNVLLKIN 613
           +KLP KPP F+LC+L E+KNS++YGPWKKK L D GI TQCI+PT++NDQY+TNVLLKIN
Sbjct: 549 SKLP-KPPLFLLCILAERKNSDVYGPWKKKNLVDLGIVTQCIAPTRLNDQYLTNVLLKIN 608

Query: 614 SKLGGINSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSDVPSIAAVVGSRSWPLISR 673
           +KLGG+NSLLA+E +  +P +   PT+I+GMDVSHGSPG+SD+PSIAAVV SR WPLIS+
Sbjct: 609 AKLGGLNSLLAMERSPAMPKVTQVPTIIVGMDVSHGSPGQSDIPSIAAVVSSRQWPLISK 668

Query: 674 YRAAVRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIILFRDGVSES 733
           Y+A VRTQS K+EMID LFKP+ G +D+G+ RELLLDFY +S  RKP  II+FRDGVSES
Sbjct: 669 YKACVRTQSRKMEMIDNLFKPVNG-KDEGMFRELLLDFYYSSENRKPEHIIIFRDGVSES 728

Query: 734 QFNQVLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPPGTVVDTKV 793
           QFNQVLNIELDQ+++A + L +   PKFTVIV QKNHHTKFFQ+  P+NVPPGT++D+++
Sbjct: 729 QFNQVLNIELDQMMQACKFLDDTWHPKFTVIVAQKNHHTKFFQSRGPDNVPPGTIIDSQI 788

Query: 794 VHPRNYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQRSTTALSIV 853
            HPRN+DFY+CAHAGMIGT+RP HYHVL DEIGF+ DDLQ L+HSLSYVYQRSTTA+S+V
Sbjct: 789 CHPRNFDFYLCAHAGMIGTTRPTHYHVLYDEIGFATDDLQELVHSLSYVYQRSTTAISVV 848

Query: 854 APICYAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAGSMFFC 906
           AP+CYAHLAA+QM   +K+E+LSETSS  G +T+ G++ +P +P+L   V+ SMFFC
Sbjct: 849 APVCYAHLAAAQMGTVMKYEELSETSSSHGGITTPGAVPVPPMPQLHNNVSTSMFFC 896

BLAST of MC04g0083 vs. TAIR 10
Match: AT5G21030.1 (PAZ domain-containing protein / piwi domain-containing protein )

HSP 1 Score: 922.2 bits (2382), Expect = 3.3e-268
Identity = 481/893 (53.86%), Postives = 626/893 (70.10%), Query Frame = 0

Query: 23  PPNMKLEKAVPPKYSI--MSRRGVGSKGRRIPLLTNHFKVSVNAPDL-IFYQYSVSISYE 82
           PP     + +  K S+  M+RRG GSKG++I LLTNHF+V+   P+   F+ YSV+I+YE
Sbjct: 7   PPQHMEREPLKSKSSLLPMTRRGNGSKGQKILLLTNHFRVNFRKPNSHNFFHYSVTITYE 66

Query: 83  DNRPVEGKGIGRKLMDQLYQTYSAELANRRFAYDGEKTLFSIGPLPQNKLEFTVVLEGSY 142
           D  P+  KG GRK+++++ QT  A+L  + FAYDG+K L+++GPLP++ L+F+VV     
Sbjct: 67  DGSPLLAKGFGRKILEKVQQTCQADLGCKHFAYDGDKNLYTVGPLPRSSLDFSVV----- 126

Query: 143 AKLETGSSGGNGSPNGIGKRSKRSFQLKTFKVELSFA-TKIPMKSIFTALKGSEVDNGST 202
             LET  S  N       KR K   Q K F V + FA  +IPM++I  AL+G +  +   
Sbjct: 127 --LETAPSRRNAD-----KRLKLPHQSKKFNVAILFAPPEIPMEAIANALQGKKTKH--L 186

Query: 203 QDALRVLDIILRQQAANRGCLLVRQSFFHDDSRNFVDVGGGVTGVRGFHSSFRLAQDGLS 262
            DA+RV+D IL Q AA +GCLLVRQSFFH+D++ F ++G GV   +GFHSSFR  Q GLS
Sbjct: 187 LDAIRVMDCILSQNAARQGCLLVRQSFFHNDAKYFANIGEGVDCCKGFHSSFRTTQGGLS 246

Query: 263 LNMDVSTTMILKPGPVIDFLIANQNVREPRYIDWAKAKRMLKNLRVKARHRNMEFKIIGL 322
           LN+DVST MI+KPGPV+DFLIANQ V +P  I+W KAK  LKNLRVK    N E+KI GL
Sbjct: 247 LNIDVSTAMIVKPGPVVDFLIANQGVNDPFSINWKKAKNTLKNLRVKVLPSNQEYKITGL 306

Query: 323 SEKPCNQQFFSMKLKNNSSTDEQMVDITVHEYFVRHCGIELTYSAYLPCLDVGKPKRPNY 382
           S   C  Q F+ K K N + + + V+ITV +YF R   IEL YS  LPC++VGKP RP Y
Sbjct: 307 SGLHCKDQTFTWK-KRNQNREFEEVEITVSDYFTRIREIELRYSGGLPCINVGKPNRPTY 366

Query: 383 LPLELCSLVSLQRYTKALSSIQRASLVEKSRQKPQEKIRIVTDALKNYRYDEDPVLAQCV 442
            P+ELC LVSLQRYTKAL+  QR++L+++SRQ PQ++I ++T ALK   Y++DP+L +C 
Sbjct: 367 FPIELCELVSLQRYTKALTKFQRSNLIKESRQNPQQRIGVLTRALKTSNYNDDPMLQECG 426

Query: 443 LKIDKQLTQLEGRVLESPKLKVGKSDDCIPRNGRWNFNNKTLLNPTRIDRWVVVNFSARC 502
           ++I    TQ+EGRVL +PKLK GK  D  P NG WNF NK    P  + RW VVNFSARC
Sbjct: 427 VRIGSDFTQVEGRVLPTPKLKAGKEQDIYPINGSWNFKNK----PATVTRWAVVNFSARC 486

Query: 503 DTSFISRELINCGRNKGIHIERPCTLI-EEDQHSRRASPVDRVENMFEQIITKLPDKPPS 562
           D   I  +L  CG+ KGI+++ P  ++ EE+   + A+   RV+ MF+ + + L + PP 
Sbjct: 487 DPQKIIDDLTRCGKMKGINVDSPYHVVFEENPQFKDATGSVRVDKMFQHLQSILGEVPPK 546

Query: 563 FILCVLPEKKNSNIY----GPWKKKCLCDFGIATQCISPTKINDQYITNVLLKINSKLGG 622
           F+LC+L EKKNS++Y      W  +C+         + P  +NDQY+TN+LLKIN+KLGG
Sbjct: 547 FLLCIL-EKKNSDVYEKSCSMWNCECI---------VPPQNLNDQYLTNLLLKINAKLGG 606

Query: 623 INSLLAIEHASCVPLIKDTPTLILGMDVSHGSPGRSD-VPSIAAVVGSRSWPLISRYRAA 682
           +NS+L +E +  +PL+   PT+I+GMDVSHGSPG+SD +PSIAAVV SR WPLIS+YRA 
Sbjct: 607 LNSVLDMELSGTMPLVMRVPTIIIGMDVSHGSPGQSDHIPSIAAVVSSREWPLISKYRAC 666

Query: 683 VRTQSPKVEMIDGLFKPLEGDQDDGIIRELLLDFYSTSNGRKPTQIILFRDGVSESQFNQ 742
           VRTQSPKVEMID LFKP+    D GI+RELLLDF+S S+G+KP  II+FRDGVSESQFNQ
Sbjct: 667 VRTQSPKVEMIDSLFKPVSDKDDQGIMRELLLDFHS-SSGKKPNHIIIFRDGVSESQFNQ 726

Query: 743 VLNIELDQIVKAYQHLGEANVPKFTVIVGQKNHHTKFFQAGAPENVPPGTVVDTKVVHPR 802
           VLNIELDQ++                   Q NHHTKFFQ  +P NV PGT++D+ + H  
Sbjct: 727 VLNIELDQMM-------------------QINHHTKFFQTESPNNVLPGTIIDSNICHQH 786

Query: 803 NYDFYMCAHAGMIGTSRPAHYHVLVDEIGFSPDDLQNLIHSLSYVYQRSTTALSIVAPIC 862
           N DFY+CAHAG IGT+RP HYHVL DEIGF  D LQ L+HSLSYVYQRSTTA+S+VAPIC
Sbjct: 787 NNDFYLCAHAGKIGTTRPTHYHVLYDEIGFDTDQLQELVHSLSYVYQRSTTAISLVAPIC 846

Query: 863 YAHLAASQMSQFIKFEDLSETSSERGTVTSAGSLSIPELPRLDKKVAGSMFFC 906
           YAHLAA+QM+  +KFED+SETSS  G +T+AG++ +P +P+L+  VA SMFFC
Sbjct: 847 YAHLAAAQMATAMKFEDMSETSSSHGGITTAGAVPVPPMPKLNTNVASSMFFC 850