MC04g0068 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0068 vs. ExPASy Swiss-Prot
Match: E0CTY1 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Vitis vinifera OX=29760 GN=GATA PE=3 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 2.9e-227
Identity = 421/551 (76.41%), Postives = 460/551 (83.48%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRF-HLHSKPLLFHTKQTP--LSSPHPIANSTLTDQIPTSTASQPLHS 60
           MLST+Q PR  L +  H   K   FH+  TP  LS P  +A+        T   S P  S
Sbjct: 1   MLSTVQNPRFLLRQCRHFPPKRRRFHS-STPLFLSQPQTLAS--------TDPPSSPPQS 60

Query: 61  QILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEE 120
           QIL+IR +L++R+ TA +LA   L RLR  EP + SF+ VSE VL +A EID KI RNEE
Sbjct: 61  QILTIRDNLINRRTTAVELAQLSLNRLRLTEPQINSFISVSETVLREAEEIDAKIARNEE 120

Query: 121 LGPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEF 180
           LGPLAGV VGVKDNICTA MPSTGGSRIL+ YRPPFDATAVKRVK  GGIVVGKTNLDEF
Sbjct: 121 LGPLAGVFVGVKDNICTADMPSTGGSRILENYRPPFDATAVKRVKDCGGIVVGKTNLDEF 180

Query: 181 GMGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCG 240
           GMGS+TEGSAFQVTANPWD+SRVPGGSSGGSA+AVSARQCVVSLGSDTGGSVRQPASFCG
Sbjct: 181 GMGSTTEGSAFQVTANPWDVSRVPGGSSGGSAAAVSARQCVVSLGSDTGGSVRQPASFCG 240

Query: 241 VVGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPD 300
           VVGLKPTYGRVSRFGLMAYASSLDVIGC G++VAD GILL+AI+G+D LDSTSSK EVP+
Sbjct: 241 VVGLKPTYGRVSRFGLMAYASSLDVIGCFGSSVADTGILLHAIAGYDGLDSTSSKHEVPN 300

Query: 301 FTSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLP 360
           +TSQF +   LESKPLKGLR+GLIRETLD+GVD  V S+I  AASHLEELGC+V EVSLP
Sbjct: 301 YTSQFMSISSLESKPLKGLRVGLIRETLDDGVDKAVVSSIVGAASHLEELGCTVTEVSLP 360

Query: 361 SFSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILM 420
           SFSLGLPAYYILASSESSSNL+RYDGVRYG+Q  AD L  LY +SRA GFG EVKMRIL 
Sbjct: 361 SFSLGLPAYYILASSESSSNLSRYDGVRYGSQVGADRLNSLYGDSRAKGFGPEVKMRILT 420

Query: 421 GTYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAM 480
           GTYALSAGYYDAYYKRAQQVRT++QKSF+AAL+ENDILISPAAPS AY IGEK NDPLAM
Sbjct: 421 GTYALSAGYYDAYYKRAQQVRTLVQKSFKAALNENDILISPAAPSPAYSIGEKKNDPLAM 480

Query: 481 YAGDIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLP 540
           YAGDIMTVNVNLAGLPALVLPCGFV  G + LPVGLQMIGAAFDE KLLKVGHIFEQ L 
Sbjct: 481 YAGDIMTVNVNLAGLPALVLPCGFVEGGPAGLPVGLQMIGAAFDEEKLLKVGHIFEQMLK 540

Query: 541 NCSFVPPLLAD 549
              F+PPLLAD
Sbjct: 541 GYKFIPPLLAD 542

BLAST of MC04g0068 vs. ExPASy Swiss-Prot
Match: Q9LI77 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GATA PE=2 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 2.1e-225
Identity = 410/548 (74.82%), Postives = 463/548 (84.49%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLHSKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQIL 60
           MLSTLQPPR SLS   L      F   +T       I ++  +  I TS  S P  SQIL
Sbjct: 1   MLSTLQPPR-SLSLLPLRR----FQISKT-------IVSAASSKTIDTSVISPP-QSQIL 60

Query: 61  SIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEELGP 120
           + R SLLS + TA ++A SYL+R+R  EP LK FLHVSE VL DA+EID +I + EELGP
Sbjct: 61  TTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEIDQRIAKGEELGP 120

Query: 121 LAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFGMG 180
           LAGVL+GVKDNICT GMPST  SRIL+ YRPPFDATAVK++K+LGGIVVGKTN+DEFGMG
Sbjct: 121 LAGVLIGVKDNICTQGMPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFGMG 180

Query: 181 SSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVG 240
           S+TE SAFQVTANPWDLSRVPGGSSGGSA+AV+ARQC+VSLGSDTGGSVRQPASFCGVVG
Sbjct: 181 STTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVG 240

Query: 241 LKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDFTS 300
           LKPTYGRVSRFGLMAYASSLDVIGC G+TVADAG+LL+AISG+D  DSTSSK++VP+F S
Sbjct: 241 LKPTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQDVPEFQS 300

Query: 301 QFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPSFS 360
           QF + D  ESKPL G+++G+IRETL++GVDS V SA + AASHLE LGC + EVSLPSFS
Sbjct: 301 QFLSVDHFESKPLNGVKVGIIRETLEDGVDSGVRSATQEAASHLEALGCILTEVSLPSFS 360

Query: 361 LGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTY 420
           LGLPAYY++ASSESSSNL+RYDGVRYGNQ +A+EL  LYE SR  GFG EVKMRILMGTY
Sbjct: 361 LGLPAYYVIASSESSSNLSRYDGVRYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTY 420

Query: 421 ALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMYAG 480
           ALSAGYYDAYYKRAQQVRT+I+K F+AAL++NDILISPAAPSAAY+IGEK +DPLAMYAG
Sbjct: 421 ALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAG 480

Query: 481 DIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPNCS 540
           DIMTVNVNLAGLPA+VLPCG V  G S LPVGLQMIGAAFDE KLLKVGHIFEQTL   S
Sbjct: 481 DIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLKGSS 535

Query: 541 FVPPLLAD 549
           FVPPLLA+
Sbjct: 541 FVPPLLAN 535

BLAST of MC04g0068 vs. ExPASy Swiss-Prot
Match: C5Y8Z8 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Sorghum bicolor OX=4558 GN=GATA PE=3 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 8.2e-206
Identity = 370/514 (71.98%), Postives = 428/514 (83.27%), Query Frame = 0

Query: 38  ANSTLTDQIPTSTASQPLHSQILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHV 97
           A S+L     T+ A  P  S ILSIR SLLS + TA  +   YL+RLR  EP L+SF+HV
Sbjct: 27  AASSLQSAPATTLAPGPATSSILSIRESLLSGERTAADITSEYLSRLRRTEPSLRSFIHV 86

Query: 98  SELVLS-DAREIDDKIQRNEE--LGPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFD 157
           ++     +A E+D +I   E+  +GPLAGVLVGVKDN+CTA MPSTGGSRILDGYRP +D
Sbjct: 87  ADAAAEREAEELDRRIASGEKDAVGPLAGVLVGVKDNLCTANMPSTGGSRILDGYRPAYD 146

Query: 158 ATAVKRVKQLGGIVVGKTNLDEFGMGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSA 217
           ATAV+R+++ G IVVGKTNLDEFGMGS+TEGSAFQVT NPWD SRVPGGSSGGSASAVSA
Sbjct: 147 ATAVRRLQEAGAIVVGKTNLDEFGMGSTTEGSAFQVTTNPWDDSRVPGGSSGGSASAVSA 206

Query: 218 RQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAG 277
           RQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDV+GC G++V D  
Sbjct: 207 RQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVVGCFGSSVFDTA 266

Query: 278 ILLNAISGHDALDSTSSKREVPDFTSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVN 337
            +L+ ++GHD +DSTSS + VPD+ S+  + DLLESKPL GLRIG+I+ETL  GV + V 
Sbjct: 267 TILSVVAGHDKMDSTSSSQVVPDYASELVSLDLLESKPLAGLRIGIIQETLGEGVANGVI 326

Query: 338 SAIRSAASHLEELGCSVNEVSLPSFSLGLPAYYILASSESSSNLARYDGVRYGNQAVADE 397
           S+I+ AASHLE+LG  V EVSLPSFSLGLPAYYILASSE+SSNL+RYDG+RYG Q  AD+
Sbjct: 327 SSIKGAASHLEQLGSVVEEVSLPSFSLGLPAYYILASSEASSNLSRYDGIRYGRQFSADD 386

Query: 398 LTGLYENSRATGFGSEVKMRILMGTYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDI 457
           L  LY  SRA G G EVKMRILMGTYALSAGYYDAYYKRAQQVRT++++SF+ AL+  DI
Sbjct: 387 LNELYGESRANGLGHEVKMRILMGTYALSAGYYDAYYKRAQQVRTLVKESFKDALERYDI 446

Query: 458 LISPAAPSAAYRIGEKVNDPLAMYAGDIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQ 517
           LISPAAPSAAY+IGEK+NDPLAMYAGDI+TVNVNLAGLPALV+PCGFV  G + LPVGLQ
Sbjct: 447 LISPAAPSAAYKIGEKINDPLAMYAGDILTVNVNLAGLPALVVPCGFVEGGPAGLPVGLQ 506

Query: 518 MIGAAFDEGKLLKVGHIFEQTLPNCSFVPPLLAD 549
           +IG+ F EG LL+VGHIFEQTL N SFVPPLLA+
Sbjct: 507 LIGSPFCEGNLLRVGHIFEQTLQNLSFVPPLLAE 540

BLAST of MC04g0068 vs. ExPASy Swiss-Prot
Match: B6U151 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Zea mays OX=4577 GN=GATA PE=2 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 1.4e-205
Identity = 371/539 (68.83%), Postives = 438/539 (81.26%), Query Frame = 0

Query: 17  LHSKPLLFHTKQTPLSSPHPIANSTLTDQIPTST-ASQPLHSQILSIRHSLLSRQITATQ 76
           L +  LL   ++ P  +      ++     P +T A  P  S ILSIR SLLS + TA  
Sbjct: 5   LQAHRLLISHRRLPTPARRRFTAASSVQSAPATTLAPGPATSSILSIRESLLSGERTAAD 64

Query: 77  LADSYLTRLRALEPHLKSFLHVSELVLS-DAREIDDKIQRNEE--LGPLAGVLVGVKDNI 136
           +   YL+RLR  EP L+SF+HV++     +A E+D +I   E+  +GPLAGVLVGVKDN+
Sbjct: 65  ITSEYLSRLRRTEPTLRSFIHVADAAAEREAEELDRRIASGEKDAVGPLAGVLVGVKDNL 124

Query: 137 CTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFGMGSSTEGSAFQVTA 196
           CTA MPSTGGSRILDGYRP +DATAV+R+++ G IV+GKTNLDEFGMGS+TEGSAFQVT 
Sbjct: 125 CTANMPSTGGSRILDGYRPAYDATAVRRLREAGAIVLGKTNLDEFGMGSTTEGSAFQVTT 184

Query: 197 NPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFG 256
           NPWD SRVPGGSSGGSA+AVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFG
Sbjct: 185 NPWDDSRVPGGSSGGSAAAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFG 244

Query: 257 LMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDFTSQFTAADLLESKP 316
           LMAYASSLDV+GC G++V D   +L+ ++GHD +D+TSS + VP++ S+  + DLLESKP
Sbjct: 245 LMAYASSLDVVGCFGSSVFDTATILSVVAGHDKMDATSSSQVVPEYASELVSLDLLESKP 304

Query: 317 LKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPSFSLGLPAYYILASS 376
           L G+RIG+I+ETL  GV S V S+I+ AASHLE+LG  V EVSLPSFSLGLPAYYILASS
Sbjct: 305 LNGVRIGIIQETLGEGVASGVVSSIKGAASHLEQLGSVVEEVSLPSFSLGLPAYYILASS 364

Query: 377 ESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTYALSAGYYDAYYK 436
           E+SSNL+RYDG+RYG Q  AD+L  LY  SRA GFG EVKMRILMGTYALSAGYYDAYYK
Sbjct: 365 EASSNLSRYDGIRYGGQVSADDLNELYGESRANGFGHEVKMRILMGTYALSAGYYDAYYK 424

Query: 437 RAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMYAGDIMTVNVNLAGL 496
           RAQQVRT++++SF+ AL+  DILISPAAPSAAY+IGEK+NDPLAMYAGDIMTVNVNLAGL
Sbjct: 425 RAQQVRTLVKQSFKDALERYDILISPAAPSAAYKIGEKINDPLAMYAGDIMTVNVNLAGL 484

Query: 497 PALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPNCSFVPPLLADDTL 552
           PALV+PCGFV  G++ LPVGLQM+G+ F EG LL+VGHIFEQTL N SFVPPLLA+  L
Sbjct: 485 PALVVPCGFVEGGAAGLPVGLQMMGSPFCEGNLLRVGHIFEQTLQNLSFVPPLLAEGQL 543

BLAST of MC04g0068 vs. ExPASy Swiss-Prot
Match: Q25A68 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=GATA PE=3 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 1.0e-203
Identity = 371/540 (68.70%), Postives = 436/540 (80.74%), Query Frame = 0

Query: 17  LHSKPLLFHTKQTPLSSPHP---IANSTLTDQIPTSTASQPLH--SQILSIRHSLLSRQI 76
           L ++ LL   ++ P  SPH     A S+L      + A+   H  S ILSIR SLLS + 
Sbjct: 5   LQAQRLLLSHRRLP--SPHRRRFTAVSSLPSSPAKTVAAAAAHAPSSILSIRESLLSGER 64

Query: 77  TATQLADSYLTRLRALEPHLKSFLHVSELVLS-DAREIDDKIQRN--EELGPLAGVLVGV 136
           TA ++   YL+RLR  EP ++SF+HV++     +A E+D +I     + +GPLAGVLVGV
Sbjct: 65  TAAEITAEYLSRLRRTEPSVRSFIHVADAAAEREAEELDRRIATEGLDAVGPLAGVLVGV 124

Query: 137 KDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFGMGSSTEGSAF 196
           KDN+CTA MPSTGGSRILDGY+P +DATAV+R+++ G IVVGKTNLDEFGMGS+TEGS F
Sbjct: 125 KDNLCTANMPSTGGSRILDGYQPAYDATAVRRLREAGAIVVGKTNLDEFGMGSTTEGSGF 184

Query: 197 QVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRV 256
           QVT NPWD SRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRV
Sbjct: 185 QVTTNPWDDSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRV 244

Query: 257 SRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDFTSQFTAADLL 316
           SRFGLMAYASSLDV+GC G++V D   +L+ I+GHD +DSTSS  +V D+ S+    DLL
Sbjct: 245 SRFGLMAYASSLDVVGCFGSSVVDTATILSVIAGHDKMDSTSSSHDVSDYKSELVPLDLL 304

Query: 317 ESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPSFSLGLPAYYI 376
           ESKPL G+RIG+I+ETL  GV++ V S+I+ AASHLE+LG  V EVSLPSFSLGLPAYYI
Sbjct: 305 ESKPLNGMRIGIIQETLGEGVETGVISSIKDAASHLEQLGSVVEEVSLPSFSLGLPAYYI 364

Query: 377 LASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTYALSAGYYD 436
           LASSE+SSNL+RYDG+RYG Q   D+L  LY  SRA G G EVKMRILMGTYALSAGYYD
Sbjct: 365 LASSEASSNLSRYDGIRYGRQVSGDDLNELYGGSRANGLGHEVKMRILMGTYALSAGYYD 424

Query: 437 AYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMYAGDIMTVNVN 496
           AYYKRAQQVRT+++KSF+ AL+  DIL+SPAAPSAAY+IGEK+NDPLAMYAGD MTVNVN
Sbjct: 425 AYYKRAQQVRTLVKKSFKEALERYDILVSPAAPSAAYKIGEKINDPLAMYAGDTMTVNVN 484

Query: 497 LAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPNCSFVPPLLAD 549
           LAGLPALV+PCGFV  GS+ LPVGLQMIG+ F EG LL++GHIFEQTL N SFVPPLLA+
Sbjct: 485 LAGLPALVVPCGFVEGGSAGLPVGLQMIGSPFSEGNLLRIGHIFEQTLQNYSFVPPLLAE 542

BLAST of MC04g0068 vs. NCBI nr
Match: XP_022148036.1 (glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 551/551 (100.00%), Postives = 551/551 (100.00%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLHSKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQIL 60
           MLSTLQPPRCSLSRFHLHSKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQIL
Sbjct: 1   MLSTLQPPRCSLSRFHLHSKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQIL 60

Query: 61  SIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEELGP 120
           SIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEELGP
Sbjct: 61  SIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEELGP 120

Query: 121 LAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFGMG 180
           LAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFGMG
Sbjct: 121 LAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFGMG 180

Query: 181 SSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVG 240
           SSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVG
Sbjct: 181 SSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVG 240

Query: 241 LKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDFTS 300
           LKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDFTS
Sbjct: 241 LKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDFTS 300

Query: 301 QFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPSFS 360
           QFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPSFS
Sbjct: 301 QFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPSFS 360

Query: 361 LGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTY 420
           LGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTY
Sbjct: 361 LGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTY 420

Query: 421 ALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMYAG 480
           ALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMYAG
Sbjct: 421 ALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMYAG 480

Query: 481 DIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPNCS 540
           DIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPNCS
Sbjct: 481 DIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPNCS 540

Query: 541 FVPPLLADDTL 551
           FVPPLLADDTL
Sbjct: 541 FVPPLLADDTL 551

BLAST of MC04g0068 vs. NCBI nr
Match: XP_038888240.1 (glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 983 bits (2541), Expect = 0.0
Identity = 507/551 (92.01%), Postives = 526/551 (95.46%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLH--SKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQ 60
           MLSTLQPPRCSLSRF LH  SKP  FH+KQTPLSS H I NSTLTDQ+PTS +SQ LHSQ
Sbjct: 1   MLSTLQPPRCSLSRFPLHLPSKPFFFHSKQTPLSSSHSIPNSTLTDQLPTSNSSQSLHSQ 60

Query: 61  ILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEEL 120
           ILSIRHSLLSR ITATQLA+SYL RLRALEPHLKSFLHVSE VLSDAREIDDKI RNEEL
Sbjct: 61  ILSIRHSLLSRHITATQLAESYLNRLRALEPHLKSFLHVSETVLSDAREIDDKIHRNEEL 120

Query: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFG 180
           GPLAGVLVGVKDNICT+GMPSTGGSRILDGYRPPFDATAVKR+K+LGGIVVGKTNLDEFG
Sbjct: 121 GPLAGVLVGVKDNICTSGMPSTGGSRILDGYRPPFDATAVKRIKELGGIVVGKTNLDEFG 180

Query: 181 MGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240
           MGSSTEGSAFQVTANPWDL+RVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV
Sbjct: 181 MGSSTEGSAFQVTANPWDLNRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240

Query: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDF 300
           VGLKPTYGRVSRFGLMAYASSLDVIGCL TTVADAGILL+AI GHD LD+TSSKREVPD 
Sbjct: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAICGHDTLDATSSKREVPDL 300

Query: 301 TSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPS 360
           TSQF+A D LESKPLKGLRIGLIRETLDNGVD +VNSAIR+AASHLEELGCS+NEVSLPS
Sbjct: 301 TSQFSAVDSLESKPLKGLRIGLIRETLDNGVDGQVNSAIRAAASHLEELGCSINEVSLPS 360

Query: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420
           FSLGLPAYYILASSESSSNLARYD VRYGNQAVADELTGLYENSRATGFGSEVKMRILMG
Sbjct: 361 FSLGLPAYYILASSESSSNLARYDSVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420

Query: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMY 480
           TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAP+AAY+IGEKV+DPLAMY
Sbjct: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPTAAYKIGEKVDDPLAMY 480

Query: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPN 540
           AGDIMTVNVNLAGLPALVLPCGFV+DGSS LPVGLQMIGAAFDEGKLLKVGHIFEQTL +
Sbjct: 481 AGDIMTVNVNLAGLPALVLPCGFVQDGSSSLPVGLQMIGAAFDEGKLLKVGHIFEQTLAD 540

Query: 541 CSFVPPLLADD 549
           C FVPPLLADD
Sbjct: 541 CRFVPPLLADD 551

BLAST of MC04g0068 vs. NCBI nr
Match: XP_023530701.1 (glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 982 bits (2538), Expect = 0.0
Identity = 505/550 (91.82%), Postives = 528/550 (96.00%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLH--SKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQ 60
           MLSTLQPPRCSLSRF LH  SKP  FH+KQTPLSSP+PIAN+TLTDQI TS +S  LHSQ
Sbjct: 1   MLSTLQPPRCSLSRFPLHLPSKPFFFHSKQTPLSSPYPIANATLTDQIATSASSPSLHSQ 60

Query: 61  ILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEEL 120
           ILSIRHSLLSRQ TATQLA+SYL RLRALEPHLKSFLHVSE VLSDAREIDDKI RNEEL
Sbjct: 61  ILSIRHSLLSRQFTATQLAESYLNRLRALEPHLKSFLHVSETVLSDAREIDDKILRNEEL 120

Query: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFG 180
           GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAV+R+K+LGGIVVGKTNLDEFG
Sbjct: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVRRIKELGGIVVGKTNLDEFG 180

Query: 181 MGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240
           MGS+TE SAFQVTANPWDL RVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV
Sbjct: 181 MGSTTEASAFQVTANPWDLKRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240

Query: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDF 300
           VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILL+AISGHD LD+TSSKREV DF
Sbjct: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLHAISGHDTLDATSSKREVDDF 300

Query: 301 TSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPS 360
           TSQFTA D LESKPLKGLRIGLIRETLDNGVD EV+SAIR+AASHLEELGCS+NEVSLPS
Sbjct: 301 TSQFTAVDSLESKPLKGLRIGLIRETLDNGVDGEVSSAIRAAASHLEELGCSINEVSLPS 360

Query: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420
           FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLY+NSRATGFGSEVKMRILMG
Sbjct: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYQNSRATGFGSEVKMRILMG 420

Query: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMY 480
           TYALS+GYYDAYYKRAQQVR+IIQKSF+AALDENDILISPAAPSAAY+IGEKV DPLAMY
Sbjct: 421 TYALSSGYYDAYYKRAQQVRSIIQKSFKAALDENDILISPAAPSAAYKIGEKVKDPLAMY 480

Query: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPN 540
           AGDIMTVNVNLAGLPALVLPCGFVRDGSS+LPVGLQMIGAAFDEGKL+KVGHIFEQTLP+
Sbjct: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSDLPVGLQMIGAAFDEGKLVKVGHIFEQTLPD 540

Query: 541 CSFVPPLLAD 548
           C FVPPLL+D
Sbjct: 541 CRFVPPLLSD 550

BLAST of MC04g0068 vs. NCBI nr
Match: XP_023530700.1 (glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 982 bits (2538), Expect = 0.0
Identity = 505/550 (91.82%), Postives = 528/550 (96.00%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLH--SKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQ 60
           MLSTLQPPRCSLSRF LH  SKP  FH+KQTPLSSP+PIAN+TLTDQI TS +S  LHSQ
Sbjct: 1   MLSTLQPPRCSLSRFPLHLPSKPFFFHSKQTPLSSPYPIANATLTDQIATSASSPSLHSQ 60

Query: 61  ILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEEL 120
           ILSIRHSLLSRQ TATQLA+SYL RLRALEPHLKSFLHVSE VLSDAREIDDKI RNEEL
Sbjct: 61  ILSIRHSLLSRQFTATQLAESYLNRLRALEPHLKSFLHVSETVLSDAREIDDKILRNEEL 120

Query: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFG 180
           GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAV+R+K+LGGIVVGKTNLDEFG
Sbjct: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVRRIKELGGIVVGKTNLDEFG 180

Query: 181 MGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240
           MGS+TE SAFQVTANPWDL RVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV
Sbjct: 181 MGSTTEASAFQVTANPWDLKRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240

Query: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDF 300
           VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILL+AISGHD LD+TSSKREV DF
Sbjct: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLHAISGHDTLDATSSKREVDDF 300

Query: 301 TSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPS 360
           TSQFTA D LESKPLKGLRIGLIRETLDNGVD EV+SAIR+AASHLEELGCS+NEVSLPS
Sbjct: 301 TSQFTAVDSLESKPLKGLRIGLIRETLDNGVDGEVSSAIRAAASHLEELGCSINEVSLPS 360

Query: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420
           FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLY+NSRATGFGSEVKMRILMG
Sbjct: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYQNSRATGFGSEVKMRILMG 420

Query: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMY 480
           TYALS+GYYDAYYKRAQQVR+IIQKSF+AALDENDILISPAAPSAAY+IGEKV DPLAMY
Sbjct: 421 TYALSSGYYDAYYKRAQQVRSIIQKSFKAALDENDILISPAAPSAAYKIGEKVKDPLAMY 480

Query: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPN 540
           AGDIMTVNVNLAGLPALVLPCGFVRDGSS+LPVGLQMIGAAFDEGKL+KVGHIFEQTLP+
Sbjct: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSDLPVGLQMIGAAFDEGKLVKVGHIFEQTLPD 540

Query: 541 CSFVPPLLAD 548
           C FVPPLL+D
Sbjct: 541 CRFVPPLLSD 550

BLAST of MC04g0068 vs. NCBI nr
Match: XP_022930890.1 (glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 979 bits (2532), Expect = 0.0
Identity = 504/550 (91.64%), Postives = 527/550 (95.82%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLH--SKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQ 60
           MLSTLQPPRCSLSRF LH  SKP  FH+K TPLSSP+PIAN+TLTDQI TS +S  LHSQ
Sbjct: 1   MLSTLQPPRCSLSRFPLHLPSKPFFFHSKPTPLSSPYPIANATLTDQIATSASSPSLHSQ 60

Query: 61  ILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEEL 120
           ILSIRHSLLSRQ TATQLA+SYL RLRALEPHLKSFLHVS+ VLSDAREIDDKI RNEEL
Sbjct: 61  ILSIRHSLLSRQFTATQLAESYLNRLRALEPHLKSFLHVSDSVLSDAREIDDKILRNEEL 120

Query: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFG 180
           GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAV+R+K+LGGIVVGKTNLDEFG
Sbjct: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVRRIKELGGIVVGKTNLDEFG 180

Query: 181 MGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240
           MGS+TE SAFQVTANPWDL RVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV
Sbjct: 181 MGSTTEASAFQVTANPWDLKRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240

Query: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDF 300
           VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILL+AISGHD LD+TSSKREV DF
Sbjct: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLHAISGHDTLDATSSKREVHDF 300

Query: 301 TSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPS 360
           TSQFTAAD LESKPLKGLRIGLIRETLDNGVD EV+SAIR+AASHLEELGCS+NEVSLPS
Sbjct: 301 TSQFTAADSLESKPLKGLRIGLIRETLDNGVDGEVSSAIRAAASHLEELGCSINEVSLPS 360

Query: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420
           FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLY+NSRATGFGSEVKMRILMG
Sbjct: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYQNSRATGFGSEVKMRILMG 420

Query: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMY 480
           TYALSAGYYDAYYKRAQQVR++IQKSF+AALDENDILISPAAPSAAY+IGEKV DPLAMY
Sbjct: 421 TYALSAGYYDAYYKRAQQVRSVIQKSFKAALDENDILISPAAPSAAYKIGEKVKDPLAMY 480

Query: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPN 540
           AGDIMTVNVNL GLPALVLPCGFVRDGSS+LPVGLQMIGAAFDEGKL+KVGHIFEQTLP+
Sbjct: 481 AGDIMTVNVNLGGLPALVLPCGFVRDGSSDLPVGLQMIGAAFDEGKLVKVGHIFEQTLPD 540

Query: 541 CSFVPPLLAD 548
           C FVPPLLAD
Sbjct: 541 CRFVPPLLAD 550

BLAST of MC04g0068 vs. ExPASy TrEMBL
Match: A0A6J1D457 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016822 PE=3 SV=1)

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 551/551 (100.00%), Postives = 551/551 (100.00%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLHSKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQIL 60
           MLSTLQPPRCSLSRFHLHSKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQIL
Sbjct: 1   MLSTLQPPRCSLSRFHLHSKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQIL 60

Query: 61  SIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEELGP 120
           SIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEELGP
Sbjct: 61  SIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEELGP 120

Query: 121 LAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFGMG 180
           LAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFGMG
Sbjct: 121 LAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFGMG 180

Query: 181 SSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVG 240
           SSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVG
Sbjct: 181 SSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVG 240

Query: 241 LKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDFTS 300
           LKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDFTS
Sbjct: 241 LKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDFTS 300

Query: 301 QFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPSFS 360
           QFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPSFS
Sbjct: 301 QFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPSFS 360

Query: 361 LGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTY 420
           LGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTY
Sbjct: 361 LGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTY 420

Query: 421 ALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMYAG 480
           ALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMYAG
Sbjct: 421 ALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMYAG 480

Query: 481 DIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPNCS 540
           DIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPNCS
Sbjct: 481 DIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPNCS 540

Query: 541 FVPPLLADDTL 551
           FVPPLLADDTL
Sbjct: 541 FVPPLLADDTL 551

BLAST of MC04g0068 vs. ExPASy TrEMBL
Match: A0A6J1EY21 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437240 PE=3 SV=1)

HSP 1 Score: 979 bits (2532), Expect = 0.0
Identity = 504/550 (91.64%), Postives = 527/550 (95.82%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLH--SKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQ 60
           MLSTLQPPRCSLSRF LH  SKP  FH+K TPLSSP+PIAN+TLTDQI TS +S  LHSQ
Sbjct: 1   MLSTLQPPRCSLSRFPLHLPSKPFFFHSKPTPLSSPYPIANATLTDQIATSASSPSLHSQ 60

Query: 61  ILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEEL 120
           ILSIRHSLLSRQ TATQLA+SYL RLRALEPHLKSFLHVS+ VLSDAREIDDKI RNEEL
Sbjct: 61  ILSIRHSLLSRQFTATQLAESYLNRLRALEPHLKSFLHVSDSVLSDAREIDDKILRNEEL 120

Query: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFG 180
           GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAV+R+K+LGGIVVGKTNLDEFG
Sbjct: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVRRIKELGGIVVGKTNLDEFG 180

Query: 181 MGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240
           MGS+TE SAFQVTANPWDL RVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV
Sbjct: 181 MGSTTEASAFQVTANPWDLKRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240

Query: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDF 300
           VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILL+AISGHD LD+TSSKREV DF
Sbjct: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLHAISGHDTLDATSSKREVHDF 300

Query: 301 TSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPS 360
           TSQFTAAD LESKPLKGLRIGLIRETLDNGVD EV+SAIR+AASHLEELGCS+NEVSLPS
Sbjct: 301 TSQFTAADSLESKPLKGLRIGLIRETLDNGVDGEVSSAIRAAASHLEELGCSINEVSLPS 360

Query: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420
           FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLY+NSRATGFGSEVKMRILMG
Sbjct: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYQNSRATGFGSEVKMRILMG 420

Query: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMY 480
           TYALSAGYYDAYYKRAQQVR++IQKSF+AALDENDILISPAAPSAAY+IGEKV DPLAMY
Sbjct: 421 TYALSAGYYDAYYKRAQQVRSVIQKSFKAALDENDILISPAAPSAAYKIGEKVKDPLAMY 480

Query: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPN 540
           AGDIMTVNVNL GLPALVLPCGFVRDGSS+LPVGLQMIGAAFDEGKL+KVGHIFEQTLP+
Sbjct: 481 AGDIMTVNVNLGGLPALVLPCGFVRDGSSDLPVGLQMIGAAFDEGKLVKVGHIFEQTLPD 540

Query: 541 CSFVPPLLAD 548
           C FVPPLLAD
Sbjct: 541 CRFVPPLLAD 550

BLAST of MC04g0068 vs. ExPASy TrEMBL
Match: A0A6J1JPA2 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111486276 PE=3 SV=1)

HSP 1 Score: 975 bits (2521), Expect = 0.0
Identity = 503/550 (91.45%), Postives = 526/550 (95.64%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLH--SKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQ 60
           MLSTLQPPR SLSRF LH  SKP  FH+KQTPLSSP+PIAN+TLTDQI TS +S  LHSQ
Sbjct: 1   MLSTLQPPRYSLSRFPLHLPSKPFFFHSKQTPLSSPYPIANATLTDQIATSASSPSLHSQ 60

Query: 61  ILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEEL 120
           ILSIRHSLLSRQ TATQLA+SYL RLRALEPHLKSFLHVS+ VLSD REIDDKI RNEEL
Sbjct: 61  ILSIRHSLLSRQFTATQLAESYLNRLRALEPHLKSFLHVSDSVLSDGREIDDKILRNEEL 120

Query: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFG 180
           GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAV+R+K+LGGIVVGKTNLDEFG
Sbjct: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVRRIKELGGIVVGKTNLDEFG 180

Query: 181 MGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240
           MGS+TE SAFQVTANPWDL RVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV
Sbjct: 181 MGSTTEASAFQVTANPWDLKRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240

Query: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDF 300
           VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILL+AISGHD LD+TSSKREV DF
Sbjct: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLHAISGHDTLDATSSKREVHDF 300

Query: 301 TSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPS 360
           TSQFTA D LESKPLKGLRIGLIRETLDNGVD +V+SAIR+AASHLEELGCS+NEVSLPS
Sbjct: 301 TSQFTAVDSLESKPLKGLRIGLIRETLDNGVDGKVSSAIRAAASHLEELGCSINEVSLPS 360

Query: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420
           FSLGLPAYYILASSESSSNLARYDGVRYGNQA+ADELTGLY+NSRATGFGSEVKMRILMG
Sbjct: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAIADELTGLYQNSRATGFGSEVKMRILMG 420

Query: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMY 480
           TYALSAGYYDAYYKRAQQVRTIIQKSF+AALDE DILISPAAPSAAY+IGEKVNDPLAMY
Sbjct: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFKAALDEYDILISPAAPSAAYKIGEKVNDPLAMY 480

Query: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPN 540
           AGDIMTVNVNLAGLPALVLPCGFVRDGSS+LPVGLQMIGAAFDEGKL+KVGHIFEQTLP+
Sbjct: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSDLPVGLQMIGAAFDEGKLVKVGHIFEQTLPD 540

Query: 541 CSFVPPLLAD 548
           C FVPPLLAD
Sbjct: 541 CRFVPPLLAD 550

BLAST of MC04g0068 vs. ExPASy TrEMBL
Match: A0A1S3BM64 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC103491372 PE=3 SV=1)

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 503/551 (91.29%), Postives = 521/551 (94.56%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLH--SKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQ 60
           MLSTLQPPRCSLSRF LH  SKP  FH+KQTPLS+PH    STLTDQ+PTS +SQPL SQ
Sbjct: 1   MLSTLQPPRCSLSRFTLHLPSKPSFFHSKQTPLSTPH----STLTDQLPTSNSSQPLQSQ 60

Query: 61  ILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEEL 120
           ILSIRHSLLSRQITATQLADSYL RLRALEPHLKSFLHVSE V SDAREIDDKI RNEEL
Sbjct: 61  ILSIRHSLLSRQITATQLADSYLDRLRALEPHLKSFLHVSETVRSDAREIDDKILRNEEL 120

Query: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFG 180
           GPLAGVLV VKDNICTAGMPST GSRILDGYRPPFDATAVK++K+LGGIV+GKTNLDEFG
Sbjct: 121 GPLAGVLVAVKDNICTAGMPSTAGSRILDGYRPPFDATAVKKIKELGGIVIGKTNLDEFG 180

Query: 181 MGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240
           MGSSTE SAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV
Sbjct: 181 MGSSTEASAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240

Query: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDF 300
           VGLKPTYGRVSRFGLMAYASSLDVIGCL TTVADAGILL+AISGHD LD+TSSKREV DF
Sbjct: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTLDATSSKREVSDF 300

Query: 301 TSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPS 360
           TSQF+A D  ESKPLKGLRIGLIRETLD GVD +VNSAIR+AASHLEELGCS+NEVSLPS
Sbjct: 301 TSQFSAVDSFESKPLKGLRIGLIRETLDKGVDGQVNSAIRAAASHLEELGCSINEVSLPS 360

Query: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420
           FSLGLPAYYILASSESSSNLARYDGVRYGNQA ADELTGLYENSRATGFGSEVKMRILMG
Sbjct: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQATADELTGLYENSRATGFGSEVKMRILMG 420

Query: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMY 480
           TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAY+IGEKV+DPLAMY
Sbjct: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYKIGEKVDDPLAMY 480

Query: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPN 540
           AGDIMTVNVNLAGLPALVLPCGFV+DGSS LPVGLQMIGAAFDEGKLLKVGHIFEQTL +
Sbjct: 481 AGDIMTVNVNLAGLPALVLPCGFVQDGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLAD 540

Query: 541 CSFVPPLLADD 549
           C FVPPLLADD
Sbjct: 541 CRFVPPLLADD 547

BLAST of MC04g0068 vs. ExPASy TrEMBL
Match: A0A6J1HZN2 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111468379 PE=3 SV=1)

HSP 1 Score: 968 bits (2502), Expect = 0.0
Identity = 503/551 (91.29%), Postives = 521/551 (94.56%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLH--SKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQ 60
           MLSTLQPPRCSLSRF LH  SKP  FH+KQTP    +PIANS LTDQ+P+S ASQ LHSQ
Sbjct: 1   MLSTLQPPRCSLSRFPLHLPSKPFFFHSKQTP----YPIANSALTDQLPSSNASQSLHSQ 60

Query: 61  ILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEEL 120
           ILSIRHSLLSRQITATQLA+SYL RLRALEPHL SFLHVSE VLSDAREID KIQRNEE+
Sbjct: 61  ILSIRHSLLSRQITATQLAESYLNRLRALEPHLNSFLHVSESVLSDAREIDGKIQRNEEV 120

Query: 121 GPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFG 180
           GPLAGV VGVKDNICTAGMPSTGGSRIL+GYRPPFDATAVKR+K+LGGIV+GKTNLDEFG
Sbjct: 121 GPLAGVFVGVKDNICTAGMPSTGGSRILEGYRPPFDATAVKRIKELGGIVIGKTNLDEFG 180

Query: 181 MGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240
           MGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV
Sbjct: 181 MGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGV 240

Query: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDF 300
           VGLKPTYGRVSRFGLMAYASSLDVIGCL TTVADAGILL+AISGHD  D+TSSKREVPDF
Sbjct: 241 VGLKPTYGRVSRFGLMAYASSLDVIGCLSTTVADAGILLHAISGHDTHDATSSKREVPDF 300

Query: 301 TSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPS 360
            SQF+A D LESKPLKGLRIGLIRETLDNGVD EVNSAIR+AASHLEELGCS+NEVSLPS
Sbjct: 301 ASQFSAVDSLESKPLKGLRIGLIRETLDNGVDGEVNSAIRAAASHLEELGCSINEVSLPS 360

Query: 361 FSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420
           FSLGLPAYYILASSE+SSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG
Sbjct: 361 FSLGLPAYYILASSEASSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMG 420

Query: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMY 480
           TYALSAGYYDAYYKRAQQVRTIIQKSFR ALDENDILISPAAPSAAY+IGEKVNDPLAMY
Sbjct: 421 TYALSAGYYDAYYKRAQQVRTIIQKSFREALDENDILISPAAPSAAYKIGEKVNDPLAMY 480

Query: 481 AGDIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPN 540
           AGDIMTVNVNLAGLPALVLPCGFVR+GSS LPVGLQMIGAAFDEGKLLKVGHIFEQTL  
Sbjct: 481 AGDIMTVNVNLAGLPALVLPCGFVRNGSSNLPVGLQMIGAAFDEGKLLKVGHIFEQTLSE 540

Query: 541 CSFVPPLLADD 549
             FVPPLLADD
Sbjct: 541 RRFVPPLLADD 547

BLAST of MC04g0068 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 783.1 bits (2021), Expect = 1.5e-226
Identity = 410/548 (74.82%), Postives = 463/548 (84.49%), Query Frame = 0

Query: 1   MLSTLQPPRCSLSRFHLHSKPLLFHTKQTPLSSPHPIANSTLTDQIPTSTASQPLHSQIL 60
           MLSTLQPPR SLS   L      F   +T       I ++  +  I TS  S P  SQIL
Sbjct: 1   MLSTLQPPR-SLSLLPLRR----FQISKT-------IVSAASSKTIDTSVISPP-QSQIL 60

Query: 61  SIRHSLLSRQITATQLADSYLTRLRALEPHLKSFLHVSELVLSDAREIDDKIQRNEELGP 120
           + R SLLS + TA ++A SYL+R+R  EP LK FLHVSE VL DA+EID +I + EELGP
Sbjct: 61  TTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEIDQRIAKGEELGP 120

Query: 121 LAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEFGMG 180
           LAGVL+GVKDNICT GMPST  SRIL+ YRPPFDATAVK++K+LGGIVVGKTN+DEFGMG
Sbjct: 121 LAGVLIGVKDNICTQGMPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFGMG 180

Query: 181 SSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVG 240
           S+TE SAFQVTANPWDLSRVPGGSSGGSA+AV+ARQC+VSLGSDTGGSVRQPASFCGVVG
Sbjct: 181 STTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVG 240

Query: 241 LKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTSSKREVPDFTS 300
           LKPTYGRVSRFGLMAYASSLDVIGC G+TVADAG+LL+AISG+D  DSTSSK++VP+F S
Sbjct: 241 LKPTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQDVPEFQS 300

Query: 301 QFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRSAASHLEELGCSVNEVSLPSFS 360
           QF + D  ESKPL G+++G+IRETL++GVDS V SA + AASHLE LGC + EVSLPSFS
Sbjct: 301 QFLSVDHFESKPLNGVKVGIIRETLEDGVDSGVRSATQEAASHLEALGCILTEVSLPSFS 360

Query: 361 LGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGLYENSRATGFGSEVKMRILMGTY 420
           LGLPAYY++ASSESSSNL+RYDGVRYGNQ +A+EL  LYE SR  GFG EVKMRILMGTY
Sbjct: 361 LGLPAYYVIASSESSSNLSRYDGVRYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTY 420

Query: 421 ALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISPAAPSAAYRIGEKVNDPLAMYAG 480
           ALSAGYYDAYYKRAQQVRT+I+K F+AAL++NDILISPAAPSAAY+IGEK +DPLAMYAG
Sbjct: 421 ALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAG 480

Query: 481 DIMTVNVNLAGLPALVLPCGFVRDGSSELPVGLQMIGAAFDEGKLLKVGHIFEQTLPNCS 540
           DIMTVNVNLAGLPA+VLPCG V  G S LPVGLQMIGAAFDE KLLKVGHIFEQTL   S
Sbjct: 481 DIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLKGSS 535

Query: 541 FVPPLLAD 549
           FVPPLLA+
Sbjct: 541 FVPPLLAN 535

BLAST of MC04g0068 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 156.4 bits (394), Expect = 6.7e-38
Identity = 120/444 (27.03%), Postives = 203/444 (45.72%), Query Frame = 0

Query: 101 VLSDAREIDDKIQRNEELGPLAGVLVGVKDNICTAGMPSTGGSRILDGYRP-PFDATAVK 160
           V+  A     + ++   +  L G+ V +KD+I     P+ GG+  L   R    D+  V 
Sbjct: 177 VIKQAEASTRRFEQGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVS 236

Query: 161 RVKQLGGIVVGKTNLDEFGMGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVV 220
           +++  G I++GK N+ E GMG++   S +  T NP D  R  GGSS GSA+ V+A  C  
Sbjct: 237 KLRSCGAILLGKANMHELGMGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSA 296

Query: 221 SLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNA 280
           +LG+D GGSVR P++ CG+ GLK TYGR    G +    ++++IG L +++ DA ++  A
Sbjct: 297 ALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAA 356

Query: 281 ISGHDALDSTSSKREVPDFTSQFTAADLLESKPLKGLRIGLIRETLDNGVDSEVNSAIRS 340
           I G  + D  + K   P F    +      S  +  LR+G   +  ++   S+++     
Sbjct: 357 ILGSSSADRYNLKPSPPCFPKLLSHNG---SNAIGSLRLGKYTKWFNDVSSSDISDKCED 416

Query: 341 AASHL-EELGCSVNEVSLPSFSLGLPAYYILASSESSSNLARYDGVRYGNQAVADELTGL 400
               L    GC V E+ +P       A+ I   S + S+L  Y      ++   D  T  
Sbjct: 417 ILKLLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKNSKLSYDTRT-- 476

Query: 401 YENSRATGFGSEVKMRILMGTYALSAGYYDAYYKRAQQVRTIIQKSFRAALDENDILISP 460
                               ++A+   +  + Y  AQ +R  + +       + D++++P
Sbjct: 477 --------------------SFAIFRSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTP 536

Query: 461 AAPSAAYRI---GEKVNDPLAMYAGDIM--TVNVNLAGLPALVLPCGFVRDGSSELPVGL 520
                A  I     K  +       D+M   +  NL G PA+ +P G+ ++G   LP+GL
Sbjct: 537 TTGMTAPVIPPDALKNGETNIQVTTDLMRFVLAANLLGFPAISVPVGYDKEG---LPIGL 592

Query: 521 QMIGAAFDEGKLLKVGHIFEQTLP 538
           Q++G  + E  +L +    E+  P
Sbjct: 597 QIMGRPWAEATVLGLAAAVEELAP 592

BLAST of MC04g0068 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 128.3 bits (321), Expect = 2.0e-29
Identity = 78/233 (33.48%), Postives = 121/233 (51.93%), Query Frame = 0

Query: 59  ILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFL-HVSELVLSDAREIDDKIQRNEE 118
           +L +   + +RQIT+ +L   YL +L+     L++ + +  EL    A+E DD + +   
Sbjct: 205 VLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTY 264

Query: 119 LGPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEF 178
           LGPL G+  G+KD +   G  +T GS          +A   KR+K  G ++V K      
Sbjct: 265 LGPLHGIPYGLKDIVAVPGYKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSM 324

Query: 179 GMGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCG 238
                  G     T NPW++     GSS G A++ SA     ++GS+T GS+  PA+ CG
Sbjct: 325 AYDDIWFGGR---TRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCG 384

Query: 239 VVGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTS 291
           +  L+PT+G V R G+M+ + SLD +G    T AD  ++L+AI G D  D +S
Sbjct: 385 ITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSS 434

BLAST of MC04g0068 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 122.1 bits (305), Expect = 1.4e-27
Identity = 78/233 (33.48%), Postives = 119/233 (51.07%), Query Frame = 0

Query: 59  ILSIRHSLLSRQITATQLADSYLTRLRALEPHLKSFL-HVSELVLSDAREIDDKIQRNEE 118
           +L +   + +RQIT+ +L   YL +L+     L++ + +  EL    A+E DD + +   
Sbjct: 205 VLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTY 264

Query: 119 LGPLAGVLVGVKDNICTAGMPSTGGSRILDGYRPPFDATAVKRVKQLGGIVVGKTNLDEF 178
           LGPL G+  G+KD +   G  +T GS          +A   KR+K  G ++V K      
Sbjct: 265 LGPLHGIPYGLKDIVAVPGYKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSM 324

Query: 179 GMGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCG 238
                  G     T NPW++     GSS G A++ SA       GS+T GS+  PA+ CG
Sbjct: 325 AYDDIWFGGR---TRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAARCG 384

Query: 239 VVGLKPTYGRVSRFGLMAYASSLDVIGCLGTTVADAGILLNAISGHDALDSTS 291
           +  L+PT+G V R G+M+ + SLD +G    T AD  ++L+AI G D  D +S
Sbjct: 385 ITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSS 427

BLAST of MC04g0068 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 92.8 bits (229), Expect = 9.1e-19
Identity = 49/110 (44.55%), Postives = 65/110 (59.09%), Query Frame = 0

Query: 155 ATAVKRVKQLGGIVVGKTNLDEFGMGSSTEGSAFQVTANPWDLSRVPGGSSGGSASAVSA 214
           A  V  + + G   +G T +DE     + E + +    NP    RVPGGSS GSA AV+A
Sbjct: 64  APVVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAA 123

Query: 215 RQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIG 265
           R    S+G+DTGGSVR PAS+CG+ G +P++G VS  GL   A S D +G
Sbjct: 124 RLVDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVG 173