MC04g0025 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0025 vs. ExPASy Swiss-Prot
Match: Q8SAB7 (Guanine nucleotide exchange factor SPIKE 1 OS=Arabidopsis thaliana OX=3702 GN=SPK1 PE=1 SV=1)

HSP 1 Score: 2906.7 bits (7534), Expect = 0.0e+00
Identity = 1475/1819 (81.09%), Postives = 1628/1819 (89.50%), Query Frame = 0

Query: 21   EENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQNQIYEGPDTDIETEMRLTYA 80
            +ENLEQWPHLN+LVQCY T+WVKD NKYGHYE I P SFQ QI+EGPDTD ETE+RL  A
Sbjct: 28   DENLEQWPHLNQLVQCYGTEWVKDVNKYGHYENIRPDSFQTQIFEGPDTDTETEIRLASA 87

Query: 81   RRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASPLPAYEPAFDWENERSMIFGQ 140
            R      T E+DV S SGRP +   DP  S   K FG  PLPAYEPAFDWENER+MIFGQ
Sbjct: 88   RSA----TIEEDVASISGRPFS---DPGSS---KHFGQPPLPAYEPAFDWENERAMIFGQ 147

Query: 141  RIPETPV---THGLKISVKVLSLSLQAGLVEPFYGTICLYNRERREKLSEDFHFRIVPKE 200
            R PE+P    + GLKISV+VLSL+ Q+GLVEPF+G+I LYN+ER+EKLSEDF+F+I P E
Sbjct: 148  RTPESPAASYSSGLKISVRVLSLAFQSGLVEPFFGSIALYNQERKEKLSEDFYFQIQPTE 207

Query: 201  MQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASVYSRKEPVHLNEREKQKL 260
            MQD K S E RG+F+L+APSASVCL IQLEK ATEEGGVT+SVYSRKEPVHL EREKQKL
Sbjct: 208  MQDAKLSSENRGVFYLDAPSASVCLLIQLEKTATEEGGVTSSVYSRKEPVHLTEREKQKL 267

Query: 261  QVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSVTGSSSHEGVLEPGTKVT 320
            QVWS+IMPYRESFAWA+V LFDN+    +  SASPSSPLAPS+T SSSH+GV EP  K+T
Sbjct: 268  QVWSRIMPYRESFAWAVVPLFDNNLTTNTGESASPSSPLAPSMTASSSHDGVYEPIAKIT 327

Query: 321  VDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPVKGVFRLEIEKHQISHAD 380
             DGK GYS GSSVVVEISNLNKVKE Y+E+++QDPK KVHKPVKGV RLEIEKH+  H D
Sbjct: 328  SDGKQGYSGGSSVVVEISNLNKVKESYSEESIQDPKRKVHKPVKGVLRLEIEKHRNGHGD 387

Query: 381  NENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSSKLNFSDGKEVSGNGSYP 440
             E++SE+GS+I+DS+D  DR  D T  K P++       +  SK N  D K+VS N +  
Sbjct: 388  FEDLSENGSIINDSLDPTDRLSDLTLMKCPSSS-SGGPRNGCSKWNSEDAKDVSRNLTSS 447

Query: 441  HGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSRKRNLFIRVELREDDSDP 500
             G  DLN   +HAFDF   TRNEPFL LFHCLYVYP+ V+LSRKRN FIRVELR+DD+D 
Sbjct: 448  CGTPDLNC--YHAFDFCSTTRNEPFLHLFHCLYVYPVAVTLSRKRNPFIRVELRKDDTDI 507

Query: 501  RRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNID 560
            R+QPLEAIYP E G SLQKW H+QVAVGAR A YHDEIK+SLPATWTP HHLLFTFF++D
Sbjct: 508  RKQPLEAIYPREPGVSLQKWVHTQVAVGARAASYHDEIKVSLPATWTPSHHLLFTFFHVD 567

Query: 561  MQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYLQDSNRERLDYLEDGKNI 620
            +Q K+EAP+PV +GYA+LPLST+   RS+ISLPVMRELVPHYLQ+S +ERLDYLEDGKNI
Sbjct: 568  LQTKLEAPRPVVVGYASLPLSTYIHSRSDISLPVMRELVPHYLQESTKERLDYLEDGKNI 627

Query: 621  FKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQFLH 680
            FKLRLRLCSSLYP NER++DF LEYDRHTL+T PPWGSELL+AINSLK+VDSTALLQFL+
Sbjct: 628  FKLRLRLCSSLYPTNERVRDFCLEYDRHTLQTRPPWGSELLQAINSLKHVDSTALLQFLY 687

Query: 681  PILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDDFGGR 740
            PILNMLLHLIGNGGETLQVAAFRAMV+I+TRVQQ S +D +RN FLV YVDY+FDDFGG 
Sbjct: 688  PILNMLLHLIGNGGETLQVAAFRAMVDILTRVQQVSFDDADRNRFLVTYVDYSFDDFGGN 747

Query: 741  QPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELIVKSMALEKTRLYYHSLP 800
            QPPVYPGL+TVWGSLARSKAKGYRVGPVYDDVL+MAWFFLELIVKSMALE+ RLY H+LP
Sbjct: 748  QPPVYPGLATVWGSLARSKAKGYRVGPVYDDVLSMAWFFLELIVKSMALEQARLYDHNLP 807

Query: 801  LGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEP 860
             GE++PPMQLKE VFRCI+QL+DCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLL IIEP
Sbjct: 808  TGEDVPPMQLKESVFRCIMQLFDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLYIIEP 867

Query: 861  RQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQE 920
             QV++LVSLY+DKFSGVCQSVLH+CKLTFLQII DHDLFVEMPGRDPSDRNYLSS+LIQE
Sbjct: 868  CQVYELVSLYMDKFSGVCQSVLHECKLTFLQIISDHDLFVEMPGRDPSDRNYLSSILIQE 927

Query: 921  LFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVF 980
            LFL+ DHD+LPLRAK ARILV+LLCKHEFDARYQK EDKLYIAQLYFP +GQILDEMPVF
Sbjct: 928  LFLSLDHDELPLRAKGARILVILLCKHEFDARYQKAEDKLYIAQLYFPFVGQILDEMPVF 987

Query: 981  YNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKLADG 1040
            YNLNA EKREVLI +LQIVRNLDDTSLVKAWQQSIARTRL+FKLMEECLILFEH+K AD 
Sbjct: 988  YNLNATEKREVLIGVLQIVRNLDDTSLVKAWQQSIARTRLYFKLMEECLILFEHKKAADS 1047

Query: 1041 MLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRPQGTPDNGYLWQRVNSQ 1100
            +L G++SR P  V +G GSPKYS+RLSPAINNYLSEASRQE R +GTPDNGYLWQRVNSQ
Sbjct: 1048 ILGGNNSRGP--VSEGAGSPKYSERLSPAINNYLSEASRQEVRLEGTPDNGYLWQRVNSQ 1107

Query: 1101 LSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQVLEIT 1160
            L+SP+QPYSLREALAQAQSSRIGASAQALRESLHP+LRQKLELWEEN+SA VSLQVLEIT
Sbjct: 1108 LASPSQPYSLREALAQAQSSRIGASAQALRESLHPILRQKLELWEENVSATVSLQVLEIT 1167

Query: 1161 EKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMAR 1220
            E FS MAASH+IATDYGKLDCIT+I  SFFS+NQ LAF+KA FP+FN +FDLHGATLMAR
Sbjct: 1168 ENFSSMAASHNIATDYGKLDCITTILTSFFSRNQSLAFWKAFFPIFNRIFDLHGATLMAR 1227

Query: 1221 ENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHFMQTARLRVMLIITLSEL 1280
            ENDRFLKQ+ FHLLRLAV+RNDS+RKRAV GLQILV+SS  +FMQTARLR +L ITLSEL
Sbjct: 1228 ENDRFLKQIAFHLLRLAVYRNDSVRKRAVIGLQILVKSSL-YFMQTARLRALLTITLSEL 1287

Query: 1281 MSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIPEASAD 1340
            MSDVQVT MK++ TLEESGEA+RL++SL +MADE+KS +LL ECGLP++ L+IIPE   +
Sbjct: 1288 MSDVQVTHMKSDNTLEESGEARRLQQSLSEMADEAKSVNLLRECGLPDDTLLIIPEKFTE 1347

Query: 1341 NKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGFYKLAMAFAPVPDLHIMW 1400
            N+WSW+E+K+LSDSL+LALDASL HALL SVM+MDRYAAAE FYKL MAFAPVPDLHIMW
Sbjct: 1348 NRWSWAEVKHLSDSLVLALDASLGHALLGSVMAMDRYAAAESFYKLGMAFAPVPDLHIMW 1407

Query: 1401 LLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRICPMVSSEIT 1460
            LLHLCDAHQEMQSWAEAAQCAVAVA V+MQALVARNDGVWS+DHV+ALR+ICPMVS E T
Sbjct: 1408 LLHLCDAHQEMQSWAEAAQCAVAVAGVIMQALVARNDGVWSKDHVSALRKICPMVSGEFT 1467

Query: 1461 SEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRRAYGQ 1520
            +EASAAEVEGYGASKLT+DSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSR+AYGQ
Sbjct: 1468 TEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRKAYGQ 1527

Query: 1521 LAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGD 1580
            LAKCH+ LT+IYESIL+QES+PIPF DATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGD
Sbjct: 1528 LAKCHTLLTNIYESILDQESNPIPFIDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGD 1587

Query: 1581 IMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQITAVDPVIEDEDLGSRRER 1640
            IMEKLSH+YESRMD +H LHIIPDSRQVKA++LQ GVCYLQITAVD V+EDEDLGSRRER
Sbjct: 1588 IMEKLSHIYESRMDSNHILHIIPDSRQVKAEDLQAGVCYLQITAVDAVMEDEDLGSRRER 1647

Query: 1641 IISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTK 1700
            I SLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTK
Sbjct: 1648 IFSLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTK 1707

Query: 1701 SESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQSLQRILQGSVAVQVNSGV 1760
            SESLEFSPVENAIGMIETRT ALRNELEEPRSS+GD LPRLQSLQRILQGSVAVQVNSGV
Sbjct: 1708 SESLEFSPVENAIGMIETRTTALRNELEEPRSSDGDHLPRLQSLQRILQGSVAVQVNSGV 1767

Query: 1761 LSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQEFHTQLVNG 1820
            LSVCTAFLSGE ATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQEFHTQLVNG
Sbjct: 1768 LSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQEFHTQLVNG 1827

Query: 1821 FQSLTAELSHYIPAILSEL 1837
            FQSLTAELSHYIPAILSEL
Sbjct: 1828 FQSLTAELSHYIPAILSEL 1830

BLAST of MC04g0025 vs. ExPASy Swiss-Prot
Match: Q8C147 (Dedicator of cytokinesis protein 8 OS=Mus musculus OX=10090 GN=Dock8 PE=1 SV=4)

HSP 1 Score: 337.0 bits (863), Expect = 1.3e-90
Identity = 480/2117 (22.67%), Postives = 826/2117 (39.02%), Query Frame = 0

Query: 17   HNKFEENLEQWPHLNELVQCYSTDWV----------------KDENKYGHYETIGPASFQ 76
            H+  EE +E  PH+ + VQ Y  +W+                K  ++    +T+   +F+
Sbjct: 105  HSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSSEFCSFKKTGSRRDFQKTLQKQTFE 164

Query: 77   NQIYEGPDTDIETEMR--------------LTYA----RRTKPEDTAEDDVPSTSGRP-E 136
            ++  E  + D +T  R              LT      R  +P++  E+ +   S    E
Sbjct: 165  SETLECSEPDTQTGPRHPLNVLCDVSGKGPLTSCDFDLRSLQPDERLENLLQLVSAEDFE 224

Query: 137  ATTYDPLLSNVPKQFGASPLPAYEPAFDWENERSMIFGQRIPETPVTH-GLKISVKVLSL 196
                +   +N P +  A     Y P     +E   +  + +PE P  H G +I VKVL+L
Sbjct: 225  KEKEEARKTNRPAELFA----LYPPV----DEEDAVEIRPVPECPKEHLGNRILVKVLTL 284

Query: 197  SLQAGLVEPFYGTICLYNRERREKLSEDFHFRIVPKEMQ----------DPKTSLEPRGI 256
              +   +EP + +I LY+ + R+K+SE+FH  +   + +          DP +S     +
Sbjct: 285  KFEIE-IEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSIDP-SSQARSAV 344

Query: 257  FFLEAPSASVCLFIQLEKHATEEGGVT-----ASVYSRKEPVHLNER-EKQKLQVWS--- 316
            F +  PS+ + L +++EK   ++G +        +    +     E+ EK KLQ  S   
Sbjct: 345  FSVTYPSSDIYLVVKIEK-VLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQ 404

Query: 317  QIMPYRESFAWAIVSL---FDNSTGAASAGSASPSSPLAPSVTGSSSHEGVLEPGTKVTV 376
            ++  YR  FAWA +SL   F+ ST    +    P      SV    S      P  +   
Sbjct: 405  RLGKYRMPFAWAPISLASFFNISTLERESTDVEPGVG-RNSVGEKRSLSQSRRPSERTLS 464

Query: 377  DGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPVKGVFRLEIEKHQISHADN 436
              + G  S        +N+   +EG   D L D         + +F+   +  + S    
Sbjct: 465  LEENGVGSNFKATTLATNIFFKQEG---DRLSD---------EDLFKFLADYKRSSSLQR 524

Query: 437  ENVSESGSV---ISDSVDLVDRQVDSTF---KKFPNNGFDSHHHSSSSKLNFSDGKEVSG 496
               S  GS+   IS + D+++  +       K FP N    H             KE+  
Sbjct: 525  RVKSIPGSLRLEISPAPDVMNCCLTPEMLPVKPFPENRTRPH-------------KEI-- 584

Query: 497  NGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSRK----RNLFIRV 556
                               +F I     P     + LYVYP  ++ + K    RN+ I++
Sbjct: 585  ------------------LEFPIREVYVPHTVYRNLLYVYPQRLNFASKLASARNITIKI 644

Query: 557  ELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLPATWTPKHH 616
            +    +      P+  I+    G    +  ++ +    +   +++E+K+ LPA  T  HH
Sbjct: 645  QFMCGEDPSNAMPV--IFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHH 704

Query: 617  LLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRS-EISLPVMRELVPHYLQDSNRER 676
            LLFTF++I  Q K  A     +GY+ LP+  + +L++    LPV  E +P      + E+
Sbjct: 705  LLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEK 764

Query: 677  -------LDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLR------------ 736
                   + + E  K +F + ++  SS++  +  ++ FF     H+L             
Sbjct: 765  VPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLC--HSLESQVSFPIRVLDQ 824

Query: 737  --TSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHL----IGNGGETLQVAAFR-- 796
              T      EL  +I  L +     L+ FLH +L+ L  L    +   G+T   + F   
Sbjct: 825  KITESTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFE 884

Query: 797  ---AMVNIVTRVQQESAEDGERNHFLVNYVDYAF--------DDFGGRQPPVYP------ 856
               A+ N +   +    +   RN  L +YV Y F            G    V P      
Sbjct: 885  SVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDTSKSGGPTTVVPDPRYHT 944

Query: 857  ----------------------------------------------------------GL 916
                                                                      G 
Sbjct: 945  YGRTSAAAVSSKLMQARVMSSSNPDLTGSHCAADEEVKNIMSSKIADRNCSRMSYYCSGN 1004

Query: 917  STVWGSLARSKAKGYR-------------VGPVYDDVLAMAWFFLELIVKSMALEKTRLY 976
            S   GS A  +    +              G V + V   AWFF EL+VKSMA      Y
Sbjct: 1005 SDAPGSTAAPRPVSKKHFHEELALQMVVSTGVVRETVFKYAWFFFELLVKSMA-----QY 1064

Query: 977  YHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHE---RCKKGLSLAKRLNSSLAFFCY 1036
             H+L   +     +  +     I  + + + +E+     + +K    A+++N SLAFF Y
Sbjct: 1065 VHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLY 1124

Query: 1037 DLLSIIEPRQVFDLVSLYLDKFSGVCQ--SVLHDCKLTFLQIICDHDLFVEM-------- 1096
            DLLSI++   VF+L+  Y  + S        L   +L FL+I+C H+ ++ +        
Sbjct: 1125 DLLSIMDRGFVFNLIKHYCSQLSAKLNILPTLISMRLEFLRILCSHEHYLNLNLLFMNTD 1184

Query: 1097 -----PGRDPSDRN--------------------------YLSSVLIQELFLTWDHDD-- 1156
                 P    S +N                          +L+ +L  EL +  D +   
Sbjct: 1185 TAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDG 1244

Query: 1157 -LPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYN------ 1216
               ++ KA   +  LLC H+ D R +KPE K+ IA LY PL+G ILD +P  Y+      
Sbjct: 1245 ISRVQRKAVSAIHSLLCSHDLDPRCRKPEVKVKIAALYLPLVGIILDALPQLYDFTDARS 1304

Query: 1217 --------------------------------------------------LNAIEKREVL 1276
                                                              LNA   R ++
Sbjct: 1305 GRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLM 1364

Query: 1277 IVILQIVRNLDDTSLVKAWQQSIARTRL--FFKLMEECLILFEHRKLADGMLIGSSSRSP 1336
            I  L I++N D  SL++ W   +   +L     L+  C+  FE++        G  S   
Sbjct: 1365 ICFLWIMKNADQ-SLIRKWIADLPSMQLNRILDLLFICVSCFEYK--------GKQS--- 1424

Query: 1337 AAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRPQGTPDNGYLWQRVNSQLSSPNQPYSL 1396
                    S K S+++     +  ++      R +G      + +R+      P    +L
Sbjct: 1425 --------SDKVSNQVLQKSRDVKAKLEEALLRGEGARGE-MMRRRIPGTDRFPGINENL 1484

Query: 1397 REALAQAQSSRIGASAQALRESLHPVLRQKLELWEE-----NLSAAVSLQVLEITEKFSL 1456
            R    Q Q           R++   + + K EL +E     NL+   +L +L++ E  ++
Sbjct: 1485 RWRKEQTQ----------WRQANEKLDKTKAELDQEALISGNLATEANLIILDMQE--NI 1544

Query: 1457 MAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMARENDRF 1516
            + AS ++      L  +  + ++  S +Q   +    F    ++    G  L   E ++ 
Sbjct: 1545 IQASSALDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQ- 1604

Query: 1517 LKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHFMQTARLRVMLIITLSELMSDVQ 1576
               +   +L       D  R +A + L +L+R SF      AR+++ + + L+ L+    
Sbjct: 1605 CADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVG--- 1664

Query: 1577 VTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIPEASADNKWSW 1636
                KA    EE      LR+SL  +   S+    +     P                  
Sbjct: 1665 ----KAPDFNEE-----HLRRSLRTILAYSEEDTAMQTTPFPMQV--------------- 1724

Query: 1637 SELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGFYKLAMAFAPVPDLHIMWLLHLC 1696
                   + LL  L++ L   +       D     +  Y++A ++   PDL + WL ++ 
Sbjct: 1725 -------EELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMA 1784

Query: 1697 DAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRICPMV--SSEITSEA 1756
            + H + + + EAA C V  AA+V + L    D  +      + + I   V   S ++ + 
Sbjct: 1785 EKHTKKKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDT 1844

Query: 1757 SAAEVEGYGASKLTLDSA-VKYLQLANKLFSQAELYHFCASILELVIPVYKSRRAYGQLA 1816
             + + +G  + +   +S  V  L+ A +LFS   LY     + +LVIP+ ++ R + +L 
Sbjct: 1845 LSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLT 1904

Query: 1817 KCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGDIM 1837
              H  L   +++I+ ++   +     TY+RVGFYG +FG LD +E+VY+EP   +L +I 
Sbjct: 1905 STHDKLQKAFDNIINKDHKRM---FGTYFRVGFYGSRFGDLDEQEFVYKEPAITKLPEIS 1964

BLAST of MC04g0025 vs. ExPASy Swiss-Prot
Match: Q8BIK4 (Dedicator of cytokinesis protein 9 OS=Mus musculus OX=10090 GN=Dock9 PE=1 SV=2)

HSP 1 Score: 331.6 bits (849), Expect = 5.5e-89
Identity = 384/1497 (25.65%), Postives = 639/1497 (42.69%), Query Frame = 0

Query: 455  IMTRNEPFLQLFHCLYVYPLTV------SLSRKRNLFIRVELREDDSDPRRQPLEAIYPV 514
            I    +P+    + LYVYP  +      S ++ RN+ I +E ++ D +   QPL+ IY  
Sbjct: 638  IPKHTQPYTVYSNHLYVYPKYLKYDSQKSFAKARNIAICIEFKDSDEED-SQPLKCIYGR 697

Query: 515  ELGASLQKWTHSQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNI--DMQAKVEAPK 574
              G    +   + V    +   ++DEIK+ LPA    +HHLLFTFF++  D   K    K
Sbjct: 698  PGGPVFTRSALAAVLHHQQNPEFYDEIKIELPAQLHERHHLLFTFFHVSCDNSTKGSTKK 757

Query: 575  PVA----IGYAALPLSTHAQ-LRSEISLPVMRELVPHYL--QDSNRER-----LDYLEDG 634
              A    +G++ LPL    + L SE  +PV   L   YL  Q+    R     + ++E G
Sbjct: 758  KDAVETQVGFSWLPLLKDGRVLTSEQHIPVSANLPSGYLGYQELGMGRHYGPEVKWVEGG 817

Query: 635  KNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQ 694
            K + K+   L S++Y  ++ + +FF +Y + T   +   GSEL++ + SL  ++   ++ 
Sbjct: 818  KPLLKISTHLVSTVYTQDQHLHNFF-QYCQKTESGAQASGSELVKYLKSLHAMEGHVMIA 877

Query: 695  FLHPILNMLLHLIGNG-GETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDD 754
            FL  ILN L  ++     E + V   R ++++V +  +E  E       L +YV +A+  
Sbjct: 878  FLPTILNQLFRVLTRATQEEVAVNVTRVIIHVVAQCHEEGLES-----HLRSYVKFAYK- 937

Query: 755  FGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVY---DDVLAMAWFFLELIVKSMALEKTR 814
                +P V     TV   L +S     +    +   + +L  +WFF ++++KSMA     
Sbjct: 938  ---AEPYVASEYKTVHEELTKSMTTILKPSADFLTSNKLLKYSWFFFDVLIKSMAQHLIE 997

Query: 815  LYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYD 874
                 L   +  P           +  + + L+  + ++ +     +K  N SLA F   
Sbjct: 998  NNKVKLLRNQRFP-----ASYHHAVETVVNMLMPHITQKFRDNPEASKNANHSLAVFIKR 1057

Query: 875  LLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEM-------PGR- 934
              + ++   VF  ++ Y+  F+      L + K  FL+++C+H+ ++ +        GR 
Sbjct: 1058 CFTFMDRGFVFKQINNYISCFAPGDPKTLFEYKFEFLRVVCNHEHYIPLNLPMPFGKGRI 1117

Query: 935  -------------DPSDRN-YLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDA 994
                         D   RN +L  +L++E+  T   +   +R  A  +L  LL KH FD 
Sbjct: 1118 QRYQDLQLDYSLTDEFCRNHFLVGLLLREVG-TALQEFREVRVIAISMLKNLLIKHSFDD 1177

Query: 995  RYQKPEDKLYIAQLYFPLIGQIL---------DEMPVFYNLNAIEKREVLIV------IL 1054
            RY     +  IA LY PL G ++         D  P   N  +I K E L V      + 
Sbjct: 1178 RYNSRSHQARIATLYLPLFGLLIENVQRINVRDVSPFPVNPGSIVKDEALAVPAGNPLMT 1237

Query: 1055 QIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKLADGMLIGSSS------RSP 1114
                N  D SL K    +I+     +      +    +   + G LI + S      R+P
Sbjct: 1238 PQKGNTLDHSLHKDLLGAISGIASPYTASTPNINSVRNAD-SRGSLISTDSGNSLPDRNP 1297

Query: 1115 ---------AAVGDGPGSPKYSDRL--------------------SPAINNYLSEASRQE 1174
                        G    S    D+L                      A+  Y ++AS  E
Sbjct: 1298 EKSNSLDKQQQSGMLGNSVVRCDKLDQSEIKSLLMCFLYVLKSMSDDALFTYWNKASTAE 1357

Query: 1175 FRPQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALR------ESLHP 1234
                 T     L      Q     + Y  R  +  A+  ++G+   ++        S   
Sbjct: 1358 LMDFFTISEVCL-----HQFQYMGKRYIARTGMMHARLQQLGSLDNSVTFNHSYGHSEAD 1417

Query: 1235 VLRQKLELWEENLSAAVSLQVLEITEKFSLMAASHSIATDYGK---LDCITSIFMSFFSK 1294
            V+ Q   L E N++  V L  L+    F+L A  + +  D+G    +  +  +++ F  K
Sbjct: 1418 VVHQ--SLLEANIATEVCLTALDTLSLFTL-AFKNQLLADHGHNPLMKKVFDVYLCFLQK 1477

Query: 1295 NQPLAFYKALFPVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGL 1354
            +Q     K +F    S+     +       D     + + +L+    +  SIR  A   L
Sbjct: 1478 HQSEMALKNVFTALRSLIYKFPSAFYEGRAD-MCASLCYEVLKCCNSKLSSIRTEASQLL 1537

Query: 1355 QILVRSSFCHFMQTA--RLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLED 1414
              L+R++F +  + +  R  + +II++S+L++DV    +   GT        R ++SL  
Sbjct: 1538 YFLMRNNFDYTGKKSFVRTHLQVIISVSQLIADV----VGIGGT--------RFQQSL-- 1597

Query: 1415 MADESKSSDLLNECGLPENALVIIPEASADNKWSWSELKYLSD---SLLLALDASLEHAL 1474
                     ++N C    N+  II   S       S++K L+    ++L+A     EH  
Sbjct: 1598 --------SIINNCA---NSDRIIKHTSFS-----SDVKDLTKRIRTVLMATAQMKEH-- 1657

Query: 1475 LASVMSMDRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAV 1534
                   D     +  Y LA ++A  P+L   WL  +   H +    +EAA C V V A+
Sbjct: 1658 -----ENDPEMLVDLQYSLAKSYASTPELRKTWLDSMARIHVKNGDLSEAAMCYVHVTAL 1717

Query: 1535 VMQALVARNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQL 1594
            V + L  +      R   TA R I P     I  EAS  E  G        D  ++ L+ 
Sbjct: 1718 VAEYLTRKG---MFRQGCTAFRVITP----NIDEEASMMEDVGMQDVHFNEDVLMELLEQ 1777

Query: 1595 ANKLFSQAELYHFCASILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTD 1654
                  +AE Y   A I +L+IP+Y+ RR + +LA  + +L   Y  + E   S      
Sbjct: 1778 CADGLWKAERYELIADIYKLIIPIYEKRRDFERLAHLYDTLHRAYSKVTEVMHSGRRLL- 1837

Query: 1655 ATYYRVGFYGEK---------------FGKLDRKEYVYREPRDVRLGDIMEKLSHVYESR 1714
             TY+RV F+G+                F   D KEY+Y+EP+   L +I ++L  +Y  +
Sbjct: 1838 GTYFRVAFFGQAAQYQFTDSETDVEGFFEDEDGKEYIYKEPKLTPLSEISQRLLKLYSDK 1897

Query: 1715 MDGSHTLHIIPDSRQVKADELQPGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRAR 1774
              GS  + +I DS +V   +L     Y+Q+T V P  ++++L  RR       T   R  
Sbjct: 1898 F-GSENVKMIQDSGKVNPKDLDSKFAYIQVTHVTPFFDEKELQERR-------TEFERCH 1957

Query: 1775 VFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENA 1827
               RF+F+ PFT+ GK QGG+E+Q KRRT+L     FP +  R+ V      + +P+E A
Sbjct: 1958 NIRRFMFEMPFTQTGKRQGGVEEQCKRRTILTAIHCFPYVKKRIPVMYQHHTDLNPIEVA 2017

BLAST of MC04g0025 vs. ExPASy Swiss-Prot
Match: Q96N67 (Dedicator of cytokinesis protein 7 OS=Homo sapiens OX=9606 GN=DOCK7 PE=1 SV=4)

HSP 1 Score: 330.9 bits (847), Expect = 9.3e-89
Identity = 452/1998 (22.62%), Postives = 776/1998 (38.84%), Query Frame = 0

Query: 142  IPETPVTH-GLKISVKVLSLSLQAGLVEPFYGTICLYNRERREKLSEDFHFRIVPKEMQD 201
            +P+ P  H G ++ VK LSL  +   +EP + ++ LY+ + ++K+SE+F+F +  ++M+ 
Sbjct: 253  VPDIPKEHFGQRLLVKCLSLKFEIE-IEPIFASLALYDVKEKKKISENFYFDLNSEQMKG 312

Query: 202  ---------PKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEG--GVTASVY-------S 261
                       T+L    IF +  PS  V L I+LEK   ++G  G  A  Y       +
Sbjct: 313  LLRPHVPPAAITTLARSAIFSITYPSQDVFLVIKLEK-VLQQGDIGECAEPYMIFKEADA 372

Query: 262  RKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSVTG 321
             K    L + + Q  Q   ++  YR  FAW  + L +     +SAGS    S      TG
Sbjct: 373  TKNKEKLEKLKSQADQFCQRLGKYRMPFAWTAIHLMNI---VSSAGSLERDSTEVEISTG 432

Query: 322  SSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYK-VHKPVK 381
                             G       SS+V   S L +   G  +DA     ++     V 
Sbjct: 433  ERK--------------GSWSERRNSSIVGRRS-LERTTSG--DDACNLTSFRPATLTVT 492

Query: 382  GVFRLEIEKHQISHAD----NENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHS 441
              F+ E ++  +S  D      ++    SV+   +  +  Q+       P N    H+  
Sbjct: 493  NFFKQEGDR--LSDEDLYKFLADMRRPSSVLR-RLRPITAQLKIDISPAPEN---PHYCL 552

Query: 442  SSSKLNFSDGKEVSGNGSYPHGIADLNAD--DFHAFDFRIMTRNEPFLQLFHCLYVYPLT 501
            +   L             YP        +  +F A D  +     P     + LY+YP +
Sbjct: 553  TPELLQVK---------LYPDSRVRPTREILEFPARDVYV-----PNTTYRNLLYIYPQS 612

Query: 502  VSLSRK----RNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACY 561
            ++ + +    RN+ ++V+    +      P+  I+     +   K  ++ V    R   +
Sbjct: 613  LNFANRQGSARNITVKVQFMYGEDPSNAMPV--IFGKSSCSEFSKEAYTAVVYHNRSPDF 672

Query: 562  HDEIKLSLPATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRS-EISLP 621
            H+EIK+ LPAT T  HHLLFTF+++  Q K   P    +GY  +P+  + +L++ +  LP
Sbjct: 673  HEEIKVKLPATLTDHHHLLFTFYHVSCQQKQNTPLETPVGYTWIPMLQNGRLKTGQFCLP 732

Query: 622  VMRELVPHYLQDSNRE----RLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFL---EYD 681
            V  E  P      + E     + ++++ K +F + +   SS++  +  +  FF      D
Sbjct: 733  VSLEKPPQAYSVLSPEVPLPGMKWVDNHKGVFNVEVVAVSSIHTQDPYLDKFFALVNALD 792

Query: 682  RHTLRT--------SPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGN----GG 741
             H                 +EL  +I++L +     +++FLH +L+ L+ L+       G
Sbjct: 793  EHLFPVRIGDMRIMENNLENELKSSISALNSSQLEPVVRFLHLLLDKLILLVIRPPVIAG 852

Query: 742  ETLQV--AAFRAMVNIVTRVQQE---SAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLS 801
            + + +  A+F AM +I+ R+ +    + +   RN  L +Y+ Y F     R P  YP  S
Sbjct: 853  QIVNLGQASFEAMASIINRLHKNLEGNHDQHGRNSLLASYIHYVF-----RLPNTYPNSS 912

Query: 802  T----------------------------------------------------------- 861
            +                                                           
Sbjct: 913  SPGPGGLGGSVHYATMARSAVRPASLNLNRSRSLSNSNPDISGTPTSPDDEVRSIIGSKG 972

Query: 862  ----------------VWGSLARSKAKGYRV----------------------------- 921
                             WGS     A+  +                              
Sbjct: 973  LDRSNSWVNTGGPKAAPWGSNPSPSAESTQAMDRSCNRMSSHTETSSFLQTLTGRLPTKK 1032

Query: 922  --------------GPVYDDVLAMAWFFLELIVKSMALEKTRLYYH---SLPLGEEIPPM 981
                          G V +  L  AWFF EL+VKSM      LY++     P     P  
Sbjct: 1033 LFHEELALQWVVCSGSVRESALQQAWFFFELMVKSMV---HHLYFNDKLEAPRKSRFPER 1092

Query: 982  QLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDLVS 1041
             + +     I  L   + +++  R +K   + +RLN+SLAFF  DLLS+++   VF L+ 
Sbjct: 1093 FMDD-----IAALVSTIASDIVSRFQKDTEMVERLNTSLAFFLNDLLSVMDRGFVFSLIK 1152

Query: 1042 LYLDKFSGVC-----QSVLHDCKLTFLQIICDHDLFVEM--------PGRDPS------- 1101
                + S         SVL   +L FL+IIC H+ +V +        P   PS       
Sbjct: 1153 SCYKQVSSKLYSLPNPSVLVSLRLDFLRIICSHEHYVTLNLPCSLLTPPASPSPSVSSAT 1212

Query: 1102 --------------------------DRNYLSSVLIQELFLTWDHDD---LPLRAKAARI 1161
                                       ++YL+ +++ EL +  D D      L  K   +
Sbjct: 1213 SQSSGFSTNVQDQKIANMFELSVPFRQQHYLAGLVLTELAVILDPDAEGLFGLHKKVINM 1272

Query: 1162 LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYN----------------- 1221
            +  LL  H+ D RY  P+ K  +A LY PLIG I++ +P  Y+                 
Sbjct: 1273 VHNLLSSHDSDPRYSDPQIKARVAMLYLPLIGIIMETVPQLYDFTETHNQRGRPICIATD 1332

Query: 1222 --------------------------------------------LNAIEKREVLIVILQI 1281
                                                         +A   R +LI +L +
Sbjct: 1333 DYESESGSMISQTVAMAIAGTSVPQLTRPGSFLLTSTSGRQHTTFSAESSRSLLICLLWV 1392

Query: 1282 VRNLDDTSLVKAWQQ-SIARTRLFFKLMEECLILFEH--RKLADGM--LIGSSSRSPAAV 1341
            ++N D+T L K +   S+ +      L+  C+  FE+  +K+ + M  L    S+   A 
Sbjct: 1393 LKNADETVLQKWFTDLSVLQLNRLLDLLYLCVSCFEYKGKKVFERMNSLTFKKSKDMRA- 1452

Query: 1342 GDGPGSPKYSDRLSPAINNYLSEASRQEFRPQGTPDNGYLWQRVNSQLSSPNQPYSLREA 1401
                       +L  AI   +        R +G        Q     ++SP +      A
Sbjct: 1453 -----------KLEEAILGSIGARQEMVRRSRG--------QLGTYTIASPPERSPSGSA 1512

Query: 1402 LAQAQSSRIGASAQALRESLHPVLRQKLELWEE-----NLSAAVSLQVLE----ITEKFS 1461
                ++ R        R++   + + + E+  E     NL+   +L +L+    + +  S
Sbjct: 1513 FGSQENLRWRKDMTHWRQNTEKLDKSRAEIEHEALIDGNLATEANLIILDTLEIVVQTVS 1572

Query: 1462 LMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMARENDR 1521
            +  +  SI      L  +  + +   + NQ   + +  F    ++       L   E ++
Sbjct: 1573 VTESKESI------LGGVLKVLLHSMACNQSAVYLQHCFATQRALVSKFPELLFEEETEQ 1632

Query: 1522 FLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHFMQTARLRVMLIITLSELMSDV 1581
                +   LLR       +IR  A + L +L+R +F      AR+++ + ++LS L+   
Sbjct: 1633 -CADLCLRLLRHCSSSIGTIRSHASASLYLLMRQNFEIGNNFARVKMQVTMSLSSLV--- 1692

Query: 1582 QVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIPEASADNKWS 1641
                    GT +   E + LR+SL+ +   ++    L E   P+                
Sbjct: 1693 --------GTSQNFNE-EFLRRSLKTILTYAEEDLELRETTFPDQV-------------- 1752

Query: 1642 WSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGFYKLAMAFAPVPDLHIMWLLHL 1701
                      L+  L   L   +       D     +  Y++A  +   PDL + WL ++
Sbjct: 1753 --------QDLVFNLHMILSDTVKMKEHQEDPEMLIDLMYRIAKGYQTSPDLRLTWLQNM 1812

Query: 1702 CDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRICPMV--SSEITSE 1761
               H E  + AEAAQC V  AA+V + L    D  +        + I   V   S ++ +
Sbjct: 1813 AGKHSERSNHAEAAQCLVHSAALVAEYLSMLEDRKYLPVGCVTFQNISSNVLEESAVSDD 1872

Query: 1762 ASAAEVEGYGASKLTLDSA-VKYLQLANKLFSQAELYHFCASILELVIPVYKSRRAYGQL 1821
              + + EG  + K   +S  V  L+ A   FS A +Y     + +++IP++++ R   +L
Sbjct: 1873 VVSPDEEGICSGKYFTESGLVGLLEQAAASFSMAGMYEAVNEVYKVLIPIHEANRDAKKL 1932

Query: 1822 AKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGDI 1837
            +  H  L + +  I+ Q S+       TY+RVGFYG KFG LD +E+VY+EP   +L +I
Sbjct: 1933 STIHGKLQEAFSKIVHQ-STGWERMFGTYFRVGFYGTKFGDLDEQEFVYKEPAITKLAEI 1992

BLAST of MC04g0025 vs. ExPASy Swiss-Prot
Match: Q9BZ29 (Dedicator of cytokinesis protein 9 OS=Homo sapiens OX=9606 GN=DOCK9 PE=1 SV=2)

HSP 1 Score: 327.8 bits (839), Expect = 7.9e-88
Identity = 364/1504 (24.20%), Postives = 636/1504 (42.29%), Query Frame = 0

Query: 455  IMTRNEPFLQLFHCLYVYPLTV------SLSRKRNLFIRVELREDDSDPRRQPLEAIYPV 514
            I    +P+    + LYVYP  +      S ++ RN+ I +E ++ D +   QPL+ IY  
Sbjct: 629  IPKHTQPYTIYTNHLYVYPKYLKYDSQKSFAKARNIAICIEFKDSDEED-SQPLKCIYGR 688

Query: 515  ELGASLQKWTHSQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNI--DMQAKVEAPK 574
              G    +   + V    +   ++DEIK+ LP     KHHLL TFF++  D  +K    K
Sbjct: 689  PGGPVFTRSAFAAVLHHHQNPEFYDEIKIELPTQLHEKHHLLLTFFHVSCDNSSKGSTKK 748

Query: 575  ----PVAIGYAALPLSTHAQ-LRSEISLPVMRELVPHYL--QDSNRER-----LDYLEDG 634
                   +GY+ LPL    + + SE  +PV   L   YL  Q+    R     + +++ G
Sbjct: 749  RDVVETQVGYSWLPLLKDGRVVTSEQHIPVSANLPSGYLGYQELGMGRHYGPEIKWVDGG 808

Query: 635  KNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQ 694
            K + K+   L S++Y  ++ + +FF +Y + T   +   G+EL++ + SL  ++   ++ 
Sbjct: 809  KPLLKISTHLVSTVYTQDQHLHNFF-QYCQKTESGAQALGNELVKYLKSLHAMEGHVMIA 868

Query: 695  FLHPILNMLLHLIGNG-GETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDD 754
            FL  ILN L  ++     E + V   R ++++V +  +E  E       L +YV YA+  
Sbjct: 869  FLPTILNQLFRVLTRATQEEVAVNVTRVIIHVVAQCHEEGLES-----HLRSYVKYAYK- 928

Query: 755  FGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVY---DDVLAMAWFFLELIVKSMALEKTR 814
                +P V     TV   L +S     +    +   + +L  +WFF ++++KSMA     
Sbjct: 929  ---AEPYVASEYKTVHEELTKSMTTILKPSADFLTSNKLLKYSWFFFDVLIKSMAQHLIE 988

Query: 815  LYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYD 874
                 L   +  P           +  + + L+  + ++ +     +K  N SLA F   
Sbjct: 989  NSKVKLLRNQRFP-----ASYHHAVETVVNMLMPHITQKFRDNPEASKNANHSLAVFIKR 1048

Query: 875  LLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEM-------PGR- 934
              + ++   VF  ++ Y+  F+      L + K  FL+++C+H+ ++ +        GR 
Sbjct: 1049 CFTFMDRGFVFKQINNYISCFAPGDPKTLFEYKFEFLRVVCNHEHYIPLNLPMPFGKGRI 1108

Query: 935  -------------DPSDRN-YLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDA 994
                         D   RN +L  +L++E+  T   +   +R  A  +L  LL KH FD 
Sbjct: 1109 QRYQDLQLDYSLTDEFCRNHFLVGLLLREVG-TALQEFREVRLIAISVLKNLLIKHSFDD 1168

Query: 995  RYQKPEDKLYIAQLYFPLIGQILDEM-----------PVFYNLNAIEKREVLIVILQIVR 1054
            RY     +  IA LY PL G +++ +           PV   +   ++   L  +  +V 
Sbjct: 1169 RYASRSHQARIATLYLPLFGLLIENVQRINVRDVSPFPVNAGMTVKDESLALPAVNPLVT 1228

Query: 1055 ----NLDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKLADGMLIGSSSRSPAAVGDG 1114
                +  D SL K    +I+     +      +    +      ++   S  S       
Sbjct: 1229 PQKGSTLDNSLHKDLLGAISGIASPYTTSTPNINSVRNADSRGSLISTDSGNSLPERNSE 1288

Query: 1115 PGSPKYSDRLSPAINNYLSEASRQE------------FRPQGTPDNGYL--WQRVNS--- 1174
              +     + S  + N +    + +            +  +   D+     W + ++   
Sbjct: 1289 KSNSLDKHQQSSTLGNSVVRCDKLDQSEIKSLLMCFLYILKSMSDDALFTYWNKASTSEL 1348

Query: 1175 ------------QLSSPNQPYSLREALAQAQSSRIGASAQALR------ESLHPVLRQKL 1234
                        Q     + Y  R  +  A+  ++G+   +L        S   VL Q  
Sbjct: 1349 MDFFTISEVCLHQFQYMGKRYIARTGMMHARLQQLGSLDNSLTFNHSYGHSDADVLHQ-- 1408

Query: 1235 ELWEENLSAAVSLQVLEITEKFSLMAASHSIATDYGK---LDCITSIFMSFFSKNQPLAF 1294
             L E N++  V L  L+    F+L A  + +  D+G    +  +  +++ F  K+Q    
Sbjct: 1409 SLLEANIATEVCLTALDTLSLFTL-AFKNQLLADHGHNPLMKKVFDVYLCFLQKHQSETA 1468

Query: 1295 YKALFPVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRS 1354
             K +F    S+     +T      D     + + +L+    +  SIR  A   L  L+R+
Sbjct: 1469 LKNVFTALRSLIYKFPSTFYEGRAD-MCAALCYEILKCCNSKLSSIRTEASQLLYFLMRN 1528

Query: 1355 SFCHFMQTA--RLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESK 1414
            +F +  + +  R  + +II++S+L++DV    +   GT        R ++SL        
Sbjct: 1529 NFDYTGKKSFVRTHLQVIISVSQLIADV----VGIGGT--------RFQQSL-------- 1588

Query: 1415 SSDLLNECGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDR 1474
               ++N C    + L+     S+D K    +L     ++L+A     EH         D 
Sbjct: 1589 --SIINNCA-NSDRLIKHTSFSSDVK----DLTKRIRTVLMATAQMKEH-------ENDP 1648

Query: 1475 YAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARN 1534
                +  Y LA ++A  P+L   WL  +   H +    +EAA C V V A+V + L  + 
Sbjct: 1649 EMLVDLQYSLAKSYASTPELRKTWLDSMARIHVKNGDLSEAAMCYVHVTALVAEYLTRKE 1708

Query: 1535 DGVWS-----RDHVTALRR-----------ICPMVSSEITSEASAAEVEGYGASKLTLDS 1594
               W        H   LRR              +++  I  EAS  E  G        D 
Sbjct: 1709 AVQWEPPLLPHSHSACLRRSRGGVFRQGCTAFRVITPNIDEEASMMEDVGMQDVHFNEDV 1768

Query: 1595 AVKYLQLANKLFSQAELYHFCASILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQES 1654
             ++ L+       +AE Y   A I +L+IP+Y+ RR + +LA  + +L   Y  + E   
Sbjct: 1769 LMELLEQCADGLWKAERYELIADIYKLIIPIYEKRRDFERLAHLYDTLHRAYSKVTEVMH 1828

Query: 1655 SPIPFTDATYYRVGFYGEK---------------FGKLDRKEYVYREPRDVRLGDIMEKL 1714
            S       TY+RV F+G+                F   D KEY+Y+EP+   L +I ++L
Sbjct: 1829 SGRRLL-GTYFRVAFFGQAAQYQFTDSETDVEGFFEDEDGKEYIYKEPKLTPLSEISQRL 1888

Query: 1715 SHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQITAVDPVIEDEDLGSRRERIISLS 1774
              +Y  +  GS  + +I DS +V   +L     Y+Q+T V P  ++++L  R+       
Sbjct: 1889 LKLYSDKF-GSENVKMIQDSGKVNPKDLDSKYAYIQVTHVIPFFDEKELQERK------- 1948

Query: 1775 TGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKSESLE 1827
            T   R+    RF+F+ PFT+ GK QGG+E+Q KRRT+L     FP +  R+ V      +
Sbjct: 1949 TEFERSHNIRRFMFEMPFTQTGKRQGGVEEQCKRRTILTAIHCFPYVKKRIPVMYQHHTD 2008

BLAST of MC04g0025 vs. NCBI nr
Match: XP_022147871.1 (guanine nucleotide exchange factor SPIKE 1 isoform X1 [Momordica charantia])

HSP 1 Score: 3619 bits (9384), Expect = 0.0
Identity = 1836/1836 (100.00%), Postives = 1836/1836 (100.00%), Query Frame = 0

Query: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60
            MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ
Sbjct: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120
            NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180
            LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE
Sbjct: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240
            RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV
Sbjct: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300
            YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300

Query: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360
            TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV
Sbjct: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360

Query: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420
            KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS
Sbjct: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420

Query: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540
            KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660
            QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260

Query: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320
            MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320

Query: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

Query: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800
            LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF
Sbjct: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800

Query: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836
            RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of MC04g0025 vs. NCBI nr
Match: XP_022147872.1 (guanine nucleotide exchange factor SPIKE 1 isoform X2 [Momordica charantia])

HSP 1 Score: 3612 bits (9367), Expect = 0.0
Identity = 1835/1836 (99.95%), Postives = 1835/1836 (99.95%), Query Frame = 0

Query: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60
            MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ
Sbjct: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120
            NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPK FGASP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPK-FGASP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180
            LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE
Sbjct: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240
            RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV
Sbjct: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300
            YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300

Query: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360
            TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV
Sbjct: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360

Query: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420
            KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS
Sbjct: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420

Query: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540
            KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660
            QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260

Query: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320
            MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320

Query: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

Query: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800
            LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF
Sbjct: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800

Query: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836
            RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1835

BLAST of MC04g0025 vs. NCBI nr
Match: XP_022147873.1 (guanine nucleotide exchange factor SPIKE 1 isoform X3 [Momordica charantia])

HSP 1 Score: 3523 bits (9136), Expect = 0.0
Identity = 1799/1836 (97.98%), Postives = 1799/1836 (97.98%), Query Frame = 0

Query: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60
            MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ
Sbjct: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120
            NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180
            LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE
Sbjct: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240
            RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV
Sbjct: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300
            YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300

Query: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360
            TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV
Sbjct: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360

Query: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420
            KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS
Sbjct: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420

Query: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540
            KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660
            QDSNRER                                     HTLRTSPPWGSELLEA
Sbjct: 601  QDSNRER-------------------------------------HTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260

Query: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320
            MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320

Query: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

Query: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800
            LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF
Sbjct: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1799

Query: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836
            RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1799

BLAST of MC04g0025 vs. NCBI nr
Match: XP_011659040.1 (guanine nucleotide exchange factor SPIKE 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 3510 bits (9102), Expect = 0.0
Identity = 1773/1836 (96.57%), Postives = 1804/1836 (98.26%), Query Frame = 0

Query: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60
            MHLLHRRD TPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGP SFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120
            NQIYEGPDTDIETEMRLTYARRTKP+DT EDDVPSTSGRPE+TTYDPLLSNVPKQ G SP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180
            LPAYEPAFDWENERSM FGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240
            RREKLSEDFHFRI PKEMQDPK S EPRGIF+LEAPSASVCLFIQLEKHATEEGGVTASV
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300
            YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPS+
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360
            TGSSSHEGV EP TKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPK+KVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420
            KGV RLEIEKHQISHADNEN+SESGSVISDSVD+VDR VDSTFKKFPNNG DSHH S SS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSHHLSGSS 420

Query: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNF  GKE SGNGS+ H   D NADDFHAFDFR+M RNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFPVGKEFSGNGSFSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540
            KRNLFIRVELREDDSDPRRQPLEA+YPVELGASLQKW H+QVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFNIDMQAK+EAPKPV IGYA+LPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660
            QD+NRERLDYLEDGKNIFKLRLRLCSSLYP+NERI+DFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRL+YHSLPLGE+IPPMQLKEGVFRCI+QLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRK ADG+L+GSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MA+SHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAV+GLQILVRSSFCHF
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320
            MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADN+WSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLT+DSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCH+ LT+IYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKA+ELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740
            QTEGSFPALVNRL+VTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLVVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

Query: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800
            LQRILQGSVAVQVNSGVLSVCTAFLSGE ATRLRSQELQQLIAALLEFMAVCKRAIRVHF
Sbjct: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800

Query: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836
            RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of MC04g0025 vs. NCBI nr
Match: XP_011659041.1 (guanine nucleotide exchange factor SPIKE 1 isoform X2 [Cucumis sativus] >KAE8646107.1 hypothetical protein Csa_015554 [Cucumis sativus])

HSP 1 Score: 3504 bits (9085), Expect = 0.0
Identity = 1772/1836 (96.51%), Postives = 1803/1836 (98.20%), Query Frame = 0

Query: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60
            MHLLHRRD TPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGP SFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120
            NQIYEGPDTDIETEMRLTYARRTKP+DT EDDVPSTSGRPE+TTYDPLLSNVPK  G SP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPK-IGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180
            LPAYEPAFDWENERSM FGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240
            RREKLSEDFHFRI PKEMQDPK S EPRGIF+LEAPSASVCLFIQLEKHATEEGGVTASV
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300
            YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPS+
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360
            TGSSSHEGV EP TKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPK+KVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420
            KGV RLEIEKHQISHADNEN+SESGSVISDSVD+VDR VDSTFKKFPNNG DSHH S SS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSHHLSGSS 420

Query: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNF  GKE SGNGS+ H   D NADDFHAFDFR+M RNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFPVGKEFSGNGSFSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540
            KRNLFIRVELREDDSDPRRQPLEA+YPVELGASLQKW H+QVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFNIDMQAK+EAPKPV IGYA+LPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660
            QD+NRERLDYLEDGKNIFKLRLRLCSSLYP+NERI+DFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRL+YHSLPLGE+IPPMQLKEGVFRCI+QLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRK ADG+L+GSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MA+SHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAV+GLQILVRSSFCHF
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320
            MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADN+WSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLT+DSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCH+ LT+IYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKA+ELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740
            QTEGSFPALVNRL+VTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLVVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

Query: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800
            LQRILQGSVAVQVNSGVLSVCTAFLSGE ATRLRSQELQQLIAALLEFMAVCKRAIRVHF
Sbjct: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800

Query: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836
            RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1835

BLAST of MC04g0025 vs. ExPASy TrEMBL
Match: A0A6J1D3N4 (guanine nucleotide exchange factor SPIKE 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016705 PE=3 SV=1)

HSP 1 Score: 3619 bits (9384), Expect = 0.0
Identity = 1836/1836 (100.00%), Postives = 1836/1836 (100.00%), Query Frame = 0

Query: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60
            MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ
Sbjct: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120
            NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180
            LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE
Sbjct: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240
            RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV
Sbjct: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300
            YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300

Query: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360
            TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV
Sbjct: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360

Query: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420
            KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS
Sbjct: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420

Query: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540
            KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660
            QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260

Query: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320
            MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320

Query: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

Query: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800
            LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF
Sbjct: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800

Query: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836
            RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of MC04g0025 vs. ExPASy TrEMBL
Match: A0A6J1D3J2 (guanine nucleotide exchange factor SPIKE 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016705 PE=3 SV=1)

HSP 1 Score: 3612 bits (9367), Expect = 0.0
Identity = 1835/1836 (99.95%), Postives = 1835/1836 (99.95%), Query Frame = 0

Query: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60
            MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ
Sbjct: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120
            NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPK FGASP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPK-FGASP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180
            LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE
Sbjct: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240
            RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV
Sbjct: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300
            YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300

Query: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360
            TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV
Sbjct: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360

Query: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420
            KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS
Sbjct: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420

Query: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540
            KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660
            QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260

Query: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320
            MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320

Query: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

Query: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800
            LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF
Sbjct: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800

Query: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836
            RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1835

BLAST of MC04g0025 vs. ExPASy TrEMBL
Match: A0A6J1D2H9 (guanine nucleotide exchange factor SPIKE 1 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016705 PE=3 SV=1)

HSP 1 Score: 3523 bits (9136), Expect = 0.0
Identity = 1799/1836 (97.98%), Postives = 1799/1836 (97.98%), Query Frame = 0

Query: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60
            MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ
Sbjct: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120
            NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180
            LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE
Sbjct: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240
            RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV
Sbjct: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300
            YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300

Query: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360
            TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV
Sbjct: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360

Query: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420
            KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS
Sbjct: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420

Query: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540
            KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660
            QDSNRER                                     HTLRTSPPWGSELLEA
Sbjct: 601  QDSNRER-------------------------------------HTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260

Query: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320
            MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320

Query: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

Query: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800
            LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF
Sbjct: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1799

Query: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836
            RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1799

BLAST of MC04g0025 vs. ExPASy TrEMBL
Match: A0A0A0K3K4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G238980 PE=3 SV=1)

HSP 1 Score: 3510 bits (9102), Expect = 0.0
Identity = 1773/1836 (96.57%), Postives = 1804/1836 (98.26%), Query Frame = 0

Query: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60
            MHLLHRRD TPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGP SFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120
            NQIYEGPDTDIETEMRLTYARRTKP+DT EDDVPSTSGRPE+TTYDPLLSNVPKQ G SP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180
            LPAYEPAFDWENERSM FGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240
            RREKLSEDFHFRI PKEMQDPK S EPRGIF+LEAPSASVCLFIQLEKHATEEGGVTASV
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300
            YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPS+
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360
            TGSSSHEGV EP TKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPK+KVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420
            KGV RLEIEKHQISHADNEN+SESGSVISDSVD+VDR VDSTFKKFPNNG DSHH S SS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSHHLSGSS 420

Query: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNF  GKE SGNGS+ H   D NADDFHAFDFR+M RNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFPVGKEFSGNGSFSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540
            KRNLFIRVELREDDSDPRRQPLEA+YPVELGASLQKW H+QVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFNIDMQAK+EAPKPV IGYA+LPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660
            QD+NRERLDYLEDGKNIFKLRLRLCSSLYP+NERI+DFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRL+YHSLPLGE+IPPMQLKEGVFRCI+QLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRK ADG+L+GSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MA+SHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAV+GLQILVRSSFCHF
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320
            MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADN+WSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLT+DSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCH+ LT+IYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKA+ELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740
            QTEGSFPALVNRL+VTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLVVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

Query: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800
            LQRILQGSVAVQVNSGVLSVCTAFLSGE ATRLRSQELQQLIAALLEFMAVCKRAIRVHF
Sbjct: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800

Query: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836
            RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of MC04g0025 vs. ExPASy TrEMBL
Match: A0A5D3BAB8 (Guanine nucleotide exchange factor SPIKE 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00800 PE=3 SV=1)

HSP 1 Score: 3497 bits (9067), Expect = 0.0
Identity = 1767/1836 (96.24%), Postives = 1798/1836 (97.93%), Query Frame = 0

Query: 1    MHLLHRRDPTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQ 60
            MHLLHRRD TPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGP SFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASP 120
            NQIYEGPDTDIETEMRLTYARRTKP+DT EDDVPSTSGRPE+TTYDPLLSNVPKQ G SP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180
            LPAYEPAFDWENERSM FGQRIPETP THGLKISVKVLSLSLQAGLVEPFYGTICLYNRE
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  RREKLSEDFHFRIVPKEMQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASV 240
            RREKLSEDFHFRI PKEMQDPK S EPRGIF+LEAPSASVCLFIQLEKHATEEGGVTASV
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSV 300
            YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPS+
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVLEPGTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPV 360
            TGSSSHEGV EP TKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPK+KVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVFRLEIEKHQISHADNENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSS 420
            KGV RLEIEKHQISHADNEN+SESGSVISDSVD+VDR VDSTFKKFPNNG DS H S SS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSQHLSCSS 420

Query: 421  KLNFSDGKEVSGNGSYPHGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSR 480
            K NF  GKE SGNGS  H   D NADDFHAFDFR+M RNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRVELREDDSDPRRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLP 540
            KRNLFIRVELREDDSDPRRQPLEA+YPVE+GASLQKW H+QVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNIDMQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFNIDMQAK+EAPKPV IGYA+LPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDSNRERLDYLEDGKNIFKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEA 660
            QD+NRERLDYLEDGKNIFKLRLRLCSSLYP+NERI+DFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLYYHSLPLGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRL+YHSLPLGE+IPPMQLKEGVFRCI+QLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNA EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNATEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKLADGMLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECL+LFEHRK ADG+L+GSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLVLFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MA+SHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHF 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAV+GLQILVRSSFCHF
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNE 1320
            MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADN+WSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLT+DSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCH+ LT+IYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKA+ELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

Query: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800
            LQRILQGSVAVQVNSGVLSVCTAFLSGE ATRLRSQELQQLIAALLEFMAVCKRAIRVHF
Sbjct: 1741 LQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHF 1800

Query: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836
            RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1801 RLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of MC04g0025 vs. TAIR 10
Match: AT4G16340.1 (guanyl-nucleotide exchange factors;GTPase binding;GTP binding )

HSP 1 Score: 2906.7 bits (7534), Expect = 0.0e+00
Identity = 1475/1819 (81.09%), Postives = 1628/1819 (89.50%), Query Frame = 0

Query: 21   EENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPASFQNQIYEGPDTDIETEMRLTYA 80
            +ENLEQWPHLN+LVQCY T+WVKD NKYGHYE I P SFQ QI+EGPDTD ETE+RL  A
Sbjct: 28   DENLEQWPHLNQLVQCYGTEWVKDVNKYGHYENIRPDSFQTQIFEGPDTDTETEIRLASA 87

Query: 81   RRTKPEDTAEDDVPSTSGRPEATTYDPLLSNVPKQFGASPLPAYEPAFDWENERSMIFGQ 140
            R      T E+DV S SGRP +   DP  S   K FG  PLPAYEPAFDWENER+MIFGQ
Sbjct: 88   RSA----TIEEDVASISGRPFS---DPGSS---KHFGQPPLPAYEPAFDWENERAMIFGQ 147

Query: 141  RIPETPV---THGLKISVKVLSLSLQAGLVEPFYGTICLYNRERREKLSEDFHFRIVPKE 200
            R PE+P    + GLKISV+VLSL+ Q+GLVEPF+G+I LYN+ER+EKLSEDF+F+I P E
Sbjct: 148  RTPESPAASYSSGLKISVRVLSLAFQSGLVEPFFGSIALYNQERKEKLSEDFYFQIQPTE 207

Query: 201  MQDPKTSLEPRGIFFLEAPSASVCLFIQLEKHATEEGGVTASVYSRKEPVHLNEREKQKL 260
            MQD K S E RG+F+L+APSASVCL IQLEK ATEEGGVT+SVYSRKEPVHL EREKQKL
Sbjct: 208  MQDAKLSSENRGVFYLDAPSASVCLLIQLEKTATEEGGVTSSVYSRKEPVHLTEREKQKL 267

Query: 261  QVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSVTGSSSHEGVLEPGTKVT 320
            QVWS+IMPYRESFAWA+V LFDN+    +  SASPSSPLAPS+T SSSH+GV EP  K+T
Sbjct: 268  QVWSRIMPYRESFAWAVVPLFDNNLTTNTGESASPSSPLAPSMTASSSHDGVYEPIAKIT 327

Query: 321  VDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPVKGVFRLEIEKHQISHAD 380
             DGK GYS GSSVVVEISNLNKVKE Y+E+++QDPK KVHKPVKGV RLEIEKH+  H D
Sbjct: 328  SDGKQGYSGGSSVVVEISNLNKVKESYSEESIQDPKRKVHKPVKGVLRLEIEKHRNGHGD 387

Query: 381  NENVSESGSVISDSVDLVDRQVDSTFKKFPNNGFDSHHHSSSSKLNFSDGKEVSGNGSYP 440
             E++SE+GS+I+DS+D  DR  D T  K P++       +  SK N  D K+VS N +  
Sbjct: 388  FEDLSENGSIINDSLDPTDRLSDLTLMKCPSSS-SGGPRNGCSKWNSEDAKDVSRNLTSS 447

Query: 441  HGIADLNADDFHAFDFRIMTRNEPFLQLFHCLYVYPLTVSLSRKRNLFIRVELREDDSDP 500
             G  DLN   +HAFDF   TRNEPFL LFHCLYVYP+ V+LSRKRN FIRVELR+DD+D 
Sbjct: 448  CGTPDLNC--YHAFDFCSTTRNEPFLHLFHCLYVYPVAVTLSRKRNPFIRVELRKDDTDI 507

Query: 501  RRQPLEAIYPVELGASLQKWTHSQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNID 560
            R+QPLEAIYP E G SLQKW H+QVAVGAR A YHDEIK+SLPATWTP HHLLFTFF++D
Sbjct: 508  RKQPLEAIYPREPGVSLQKWVHTQVAVGARAASYHDEIKVSLPATWTPSHHLLFTFFHVD 567

Query: 561  MQAKVEAPKPVAIGYAALPLSTHAQLRSEISLPVMRELVPHYLQDSNRERLDYLEDGKNI 620
            +Q K+EAP+PV +GYA+LPLST+   RS+ISLPVMRELVPHYLQ+S +ERLDYLEDGKNI
Sbjct: 568  LQTKLEAPRPVVVGYASLPLSTYIHSRSDISLPVMRELVPHYLQESTKERLDYLEDGKNI 627

Query: 621  FKLRLRLCSSLYPVNERIKDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQFLH 680
            FKLRLRLCSSLYP NER++DF LEYDRHTL+T PPWGSELL+AINSLK+VDSTALLQFL+
Sbjct: 628  FKLRLRLCSSLYPTNERVRDFCLEYDRHTLQTRPPWGSELLQAINSLKHVDSTALLQFLY 687

Query: 681  PILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDDFGGR 740
            PILNMLLHLIGNGGETLQVAAFRAMV+I+TRVQQ S +D +RN FLV YVDY+FDDFGG 
Sbjct: 688  PILNMLLHLIGNGGETLQVAAFRAMVDILTRVQQVSFDDADRNRFLVTYVDYSFDDFGGN 747

Query: 741  QPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELIVKSMALEKTRLYYHSLP 800
            QPPVYPGL+TVWGSLARSKAKGYRVGPVYDDVL+MAWFFLELIVKSMALE+ RLY H+LP
Sbjct: 748  QPPVYPGLATVWGSLARSKAKGYRVGPVYDDVLSMAWFFLELIVKSMALEQARLYDHNLP 807

Query: 801  LGEEIPPMQLKEGVFRCILQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEP 860
             GE++PPMQLKE VFRCI+QL+DCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLL IIEP
Sbjct: 808  TGEDVPPMQLKESVFRCIMQLFDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLYIIEP 867

Query: 861  RQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQE 920
             QV++LVSLY+DKFSGVCQSVLH+CKLTFLQII DHDLFVEMPGRDPSDRNYLSS+LIQE
Sbjct: 868  CQVYELVSLYMDKFSGVCQSVLHECKLTFLQIISDHDLFVEMPGRDPSDRNYLSSILIQE 927

Query: 921  LFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVF 980
            LFL+ DHD+LPLRAK ARILV+LLCKHEFDARYQK EDKLYIAQLYFP +GQILDEMPVF
Sbjct: 928  LFLSLDHDELPLRAKGARILVILLCKHEFDARYQKAEDKLYIAQLYFPFVGQILDEMPVF 987

Query: 981  YNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKLADG 1040
            YNLNA EKREVLI +LQIVRNLDDTSLVKAWQQSIARTRL+FKLMEECLILFEH+K AD 
Sbjct: 988  YNLNATEKREVLIGVLQIVRNLDDTSLVKAWQQSIARTRLYFKLMEECLILFEHKKAADS 1047

Query: 1041 MLIGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRPQGTPDNGYLWQRVNSQ 1100
            +L G++SR P  V +G GSPKYS+RLSPAINNYLSEASRQE R +GTPDNGYLWQRVNSQ
Sbjct: 1048 ILGGNNSRGP--VSEGAGSPKYSERLSPAINNYLSEASRQEVRLEGTPDNGYLWQRVNSQ 1107

Query: 1101 LSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQVLEIT 1160
            L+SP+QPYSLREALAQAQSSRIGASAQALRESLHP+LRQKLELWEEN+SA VSLQVLEIT
Sbjct: 1108 LASPSQPYSLREALAQAQSSRIGASAQALRESLHPILRQKLELWEENVSATVSLQVLEIT 1167

Query: 1161 EKFSLMAASHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMAR 1220
            E FS MAASH+IATDYGKLDCIT+I  SFFS+NQ LAF+KA FP+FN +FDLHGATLMAR
Sbjct: 1168 ENFSSMAASHNIATDYGKLDCITTILTSFFSRNQSLAFWKAFFPIFNRIFDLHGATLMAR 1227

Query: 1221 ENDRFLKQVTFHLLRLAVFRNDSIRKRAVSGLQILVRSSFCHFMQTARLRVMLIITLSEL 1280
            ENDRFLKQ+ FHLLRLAV+RNDS+RKRAV GLQILV+SS  +FMQTARLR +L ITLSEL
Sbjct: 1228 ENDRFLKQIAFHLLRLAVYRNDSVRKRAVIGLQILVKSSL-YFMQTARLRALLTITLSEL 1287

Query: 1281 MSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIPEASAD 1340
            MSDVQVT MK++ TLEESGEA+RL++SL +MADE+KS +LL ECGLP++ L+IIPE   +
Sbjct: 1288 MSDVQVTHMKSDNTLEESGEARRLQQSLSEMADEAKSVNLLRECGLPDDTLLIIPEKFTE 1347

Query: 1341 NKWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGFYKLAMAFAPVPDLHIMW 1400
            N+WSW+E+K+LSDSL+LALDASL HALL SVM+MDRYAAAE FYKL MAFAPVPDLHIMW
Sbjct: 1348 NRWSWAEVKHLSDSLVLALDASLGHALLGSVMAMDRYAAAESFYKLGMAFAPVPDLHIMW 1407

Query: 1401 LLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRICPMVSSEIT 1460
            LLHLCDAHQEMQSWAEAAQCAVAVA V+MQALVARNDGVWS+DHV+ALR+ICPMVS E T
Sbjct: 1408 LLHLCDAHQEMQSWAEAAQCAVAVAGVIMQALVARNDGVWSKDHVSALRKICPMVSGEFT 1467

Query: 1461 SEASAAEVEGYGASKLTLDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRRAYGQ 1520
            +EASAAEVEGYGASKLT+DSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSR+AYGQ
Sbjct: 1468 TEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRKAYGQ 1527

Query: 1521 LAKCHSSLTDIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGD 1580
            LAKCH+ LT+IYESIL+QES+PIPF DATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGD
Sbjct: 1528 LAKCHTLLTNIYESILDQESNPIPFIDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGD 1587

Query: 1581 IMEKLSHVYESRMDGSHTLHIIPDSRQVKADELQPGVCYLQITAVDPVIEDEDLGSRRER 1640
            IMEKLSH+YESRMD +H LHIIPDSRQVKA++LQ GVCYLQITAVD V+EDEDLGSRRER
Sbjct: 1588 IMEKLSHIYESRMDSNHILHIIPDSRQVKAEDLQAGVCYLQITAVDAVMEDEDLGSRRER 1647

Query: 1641 IISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTK 1700
            I SLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTK
Sbjct: 1648 IFSLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTK 1707

Query: 1701 SESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQSLQRILQGSVAVQVNSGV 1760
            SESLEFSPVENAIGMIETRT ALRNELEEPRSS+GD LPRLQSLQRILQGSVAVQVNSGV
Sbjct: 1708 SESLEFSPVENAIGMIETRTTALRNELEEPRSSDGDHLPRLQSLQRILQGSVAVQVNSGV 1767

Query: 1761 LSVCTAFLSGESATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQEFHTQLVNG 1820
            LSVCTAFLSGE ATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQEFHTQLVNG
Sbjct: 1768 LSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQEFHTQLVNG 1827

Query: 1821 FQSLTAELSHYIPAILSEL 1837
            FQSLTAELSHYIPAILSEL
Sbjct: 1828 FQSLTAELSHYIPAILSEL 1830