MC03g_new0335 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0335 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 1.2e-229
Identity = 393/613 (64.11%), Postives = 486/613 (79.28%), Query Frame = 0

Query: 4   NVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPPGIV 63
           N  R D  SAAEKAVSV+G GYDLC+D+R S+CK  P GS+L+ +DPTR +DL+ P GIV
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNWQND 123
           V NVS+SIKCDKGERTR RSD++SFNQMSE+FNQ +CLSGKIPSG FN MF F   W  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S K+LA+DG FI+LY++E+ R  +TL +++KREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDEYSV 243
            MGGKDVI+++Q+R+SN E  E+QK LK   D+RF  D   + S  S   + K K+E  +
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPAS-VYSGKPKEENLL 244

Query: 244 PWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISMSFV 303
            W L+  F  S+   +V H+K++E+M++ +RRGGVD+GQSH +WLST+SQ+PNVISM FV
Sbjct: 245 QWGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFV 304

Query: 304 PITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRNQ 363
           PITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R   Q
Sbjct: 305 PITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQ 364

Query: 364 ASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTLQLS 423
           +SPSL+F+LMGP+LYVNT+KVD+  RPVTG+R FLEGKK +HLA+HLQHLS+ P +L LS
Sbjct: 365 SSPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLS 424

Query: 424 DDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKVVGM 483
            D +Y+PI+EP EKGY+ PVKW IFSHVCT PV+YNG+   DD+ASIVTKAW EVK +GM
Sbjct: 425 HDDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGM 484

Query: 484 RKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLH----QPEKQPK 543
           RKVLFLRLGFS+ ASAV R+S W+  ST SRKSG+ S  ISTR STGL       + Q K
Sbjct: 485 RKVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSK 544

Query: 544 IELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVK 603
           I++NSAVYP GP    +  K+L++++TKE++RGPE  PGYWVVTGAKLCVE G+IS+K K
Sbjct: 545 IDINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAK 604

Query: 604 YALLTILSEDSMI 613
           Y+LLT++SEDS++
Sbjct: 605 YSLLTVISEDSLV 612

BLAST of MC03g_new0335 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 6.8e-177
Identity = 319/611 (52.21%), Postives = 423/611 (69.23%), Query Frame = 0

Query: 7   RFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHL-DPTRTKDLVLPPGIVVP 66
           R     AAE A+  +G GYDL  D+RL  CK G   S+L+ + +     ++VLP GI +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNWQNDAG 126
           NVS SIKCDKGER RFRSD++ F QM+EQFNQ+L L+GKIPSG FNAMF F   WQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            TK+LAFDG FI+LY++ L++S + L E +K+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDA--NATGSFTSHPAAPKIKDEYSV 246
           GGKDVIY +Q   S L+  ++QK LK++AD RF E +  + TGS     A+ K++ +   
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGS-ERVQASSKVETK--- 243

Query: 247 PWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVD-IGQSHNQWLSTISQSPNVISMSF 306
             E R  FA +    + S++  ++ + +  RRGG D     HN+WL T+   P+VISMSF
Sbjct: 244 --EQRLRFADT--SSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSF 303

Query: 307 VPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRN 366
           +PITSL+NG+ G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL  + + 
Sbjct: 304 IPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQ 363

Query: 367 QASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTLQL 426
           Q+  SL+F+  GP+LYVNTT VD   RP+TG+RL+LEG++S+ LA+HLQHLSSLPK  QL
Sbjct: 364 QSCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQL 423

Query: 427 SDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKVVG 486
            DDL+     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G
Sbjct: 424 EDDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHG 483

Query: 487 MRKVLFLRLGFSMVASA-VIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKIE 546
            + VLFLRL FS V  A +++ SEW+     + KSGLIST IS  F+     P +   + 
Sbjct: 484 FKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVN 543

Query: 547 LNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKYA 606
           +NSA+YP GPP+ ++A K+L  ++T EM RGP+ SPGYWVV+GA+L VE G+IS+KVKY+
Sbjct: 544 INSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYS 600

Query: 607 LLTILSEDSMI 613
           L T +  D +I
Sbjct: 604 LFTPILGDEVI 600

BLAST of MC03g_new0335 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 7.6e-128
Identity = 251/621 (40.42%), Postives = 382/621 (61.51%), Query Frame = 0

Query: 9   DPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGS---KLIHLDPTRTKDLVLPPGIVVP 68
           D     E AV  LG G+DL  D RL  CK G   +   +L+ LD T+ ++L +P   V  
Sbjct: 4   DGGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQ 63

Query: 69  NVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFK-GNWQNDA 128
           NVS  I CDKGERTRFRSD++ FN+MSE FNQ+  ++GKIPSG FNA F F+ G+W  DA
Sbjct: 64  NVSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDA 123

Query: 129 GSTKSLAFDGCFITLYNIELER-SHITLSEQLKREVPSSWDPAALAEFIEKYGTHVVVGV 188
            + KSL  D   +TL+N+ +   + + L+++++  VPSSWDP  LA FIE+YGTHV+ GV
Sbjct: 124 ANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGV 183

Query: 189 KMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFS-----EDANATGSFTSHPAAPKIK 248
            +GG+DV+ +RQ + S+L+ + ++  L  L D  F+            ++    + PK  
Sbjct: 184 SVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFP 243

Query: 249 DEYSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVI 308
           + ++V ++ +   A +      S +  + +  I  +RGG    +SH++WL T+   P+ I
Sbjct: 244 EAFNV-FDDKQTVAFN----NFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAI 303

Query: 309 SMSFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-A 368
           + +F+PITSL+  + G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A
Sbjct: 304 NFNFIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGA 363

Query: 369 TRHRNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLP 428
             +   A P+L    MGP+LYVNTT V ++  PVTG+R FLEGKK + LA+HLQHL +  
Sbjct: 364 APNMASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTR 423

Query: 429 KTL--QLSDDLSYKPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYNGSLI--------DDD 488
            T+  +++D+  ++  D+  +   YFEP+    FSHVCT PV+Y+ + I         +D
Sbjct: 424 TTVGEKITDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQND 483

Query: 489 SASIVTKAWFEVKVVGMRKVLFLRLGFSMVASAVIRRSEW-EGPSTTSRKSGLISTFIST 548
            A IVT A  EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+    
Sbjct: 484 VAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMP 543

Query: 549 RFSTGLHQ---PEKQPKIELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVV 604
             S  +H     + + ++ L+S V+P GPP+ +   K++  ++  ++ RGP++SPG+W+V
Sbjct: 544 LTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLV 603

BLAST of MC03g_new0335 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.9e-118
Identity = 239/602 (39.70%), Postives = 350/602 (58.14%), Query Frame = 0

Query: 17  AVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPPGIVVPNVSTSIKCDKG 76
           A+  LG G+D+ +D+RL  CK  P GS+L+ ++  + +DL L  G ++PNV   I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNWQNDAGSTKSLAFDGCF 136
                R  V SF++M+E+FN +  + G IP G FNAMFN+ G+WQ DA STKSLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIYMRQL 196
           I LY+++L +  + L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+Y+RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDEYSVPWELRNAFAASIK 256
           + S L  +E++  +  +   RF E                             A + SI 
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHE-----------------------------AESQSIT 263

Query: 257 PPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISMSFVPITSLMNGIRGNG 316
            P+    K  ++  IF RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ G+ G  
Sbjct: 264 GPL--KYKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLR 323

Query: 317 FLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRNQASPSLKFTLMGPR 376
            L+ A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + +     SL+F+LMGP+
Sbjct: 324 HLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPK 383

Query: 377 LYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTLQLSDDLSYKPIDEPEE 436
           L+++  +V    +PVTG+RL LEG K + L++HLQHL SLPK LQ   D S+ PI  P+ 
Sbjct: 384 LFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKW 443

Query: 437 KG-------YFEPVKWSIFSHVCTAPVEYNGSLIDD-DSASIVTKAWFEVKVVGMRKVLF 496
           +G       +FEP+KW  FSHV T+P+E+  + I D     IVT A   V   G + VL 
Sbjct: 444 QGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLH 503

Query: 497 LRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKIELNSAVYP 556
           L+L FS V    IRRS W+                        H P     +  +  + P
Sbjct: 504 LKLLFSKVPGCTIRRSVWD------------------------HTP-----VASSGRLEP 559

Query: 557 DGPPLTS-------RAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKYA 604
            GP  +S       ++ K+  ++++ EM++GP++ PG+W+VTGAKL VE G+I ++VKY+
Sbjct: 564 GGPSTSSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYS 559

BLAST of MC03g_new0335 vs. NCBI nr
Match: XP_022140066.1 (MACPF domain-containing protein NSL1 [Momordica charantia])

HSP 1 Score: 1226 bits (3172), Expect = 0.0
Identity = 612/612 (100.00%), Postives = 612/612 (100.00%), Query Frame = 0

Query: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60
           MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP
Sbjct: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60

Query: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120
           GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW
Sbjct: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120

Query: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240
           VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE
Sbjct: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240

Query: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300
           YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM
Sbjct: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300

Query: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360
           SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH
Sbjct: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360

Query: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420
           RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL
Sbjct: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420

Query: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480
           QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV
Sbjct: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480

Query: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540
           VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI
Sbjct: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600
           ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY
Sbjct: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600

Query: 601 ALLTILSEDSMI 612
           ALLTILSEDSMI
Sbjct: 601 ALLTILSEDSMI 612

BLAST of MC03g_new0335 vs. NCBI nr
Match: XP_038894492.1 (MACPF domain-containing protein NSL1 [Benincasa hispida])

HSP 1 Score: 1120 bits (2896), Expect = 0.0
Identity = 548/612 (89.54%), Postives = 588/612 (96.08%), Query Frame = 0

Query: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60
           MSFN++RFDPQSAAEKAVSV+GFGYDLCND+RLSSCKPGPSGSKLI +D TRT+DLVLP 
Sbjct: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRTRDLVLPG 60

Query: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120
           G+VVPNVSTSI CDKGERTRFRSDVISFNQMSE FNQQL LSGKIPSGFFN MF  KG W
Sbjct: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGST+SLAFDGCFITLYNIELE+SHITLSEQ+KREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240
           VGVKMGGKDVI+MRQLRESN+EQ +VQK LK+LAD++FSEDAN  G FTS+PAA KIKDE
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDAN--GGFTSNPAAAKIKDE 240

Query: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300
           YS+PWELRNAFAASIKPP+++HSK DEL  I++RRGGVD+GQSHNQWLSTISQSPNVISM
Sbjct: 241 YSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISM 300

Query: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360
           SFVPITSLMNG+RGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RH
Sbjct: 301 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRH 360

Query: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420
           R QASPSLKFTLMGP+LYVNTTKVD+ NRPVTG+RLFLEGKKSDHLA+HLQHLS+LPKTL
Sbjct: 361 RKQASPSLKFTLMGPKLYVNTTKVDSHNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL 420

Query: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480
           QLSDDLSY+PIDEPEEKGYFEPV+WSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV
Sbjct: 421 QLSDDLSYRPIDEPEEKGYFEPVRWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480

Query: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540
           +GMRKVLFLRLGFSMVASA IRRS+WEGPSTTSRKSGLISTFISTRFS+GLHQPE+QPKI
Sbjct: 481 IGMRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSSGLHQPEEQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600
           ELNSAVYPDGPPLTSRAMKMLN+I+TKEMVRGPENSPGYWVVTGAKLCVEG RIS+KVKY
Sbjct: 541 ELNSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKY 600

Query: 601 ALLTILSEDSMI 612
           +LLTILSEDSMI
Sbjct: 601 SLLTILSEDSMI 610

BLAST of MC03g_new0335 vs. NCBI nr
Match: TYK26438.1 (MACPF domain-containing protein NSL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1110 bits (2871), Expect = 0.0
Identity = 545/612 (89.05%), Postives = 584/612 (95.42%), Query Frame = 0

Query: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60
           MSFN++RF+PQSAAEKAVSV+GFGYDLCND+RLSSCKPGPSGSKLI +D TR++DLVLP 
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120
           G+VVPNV TSI CDKGERTRFRSDVISFNQMSE FNQQL LSGKIPSGFFN MF  KG W
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGST+SLAFDGCFITLYNIELE+SHITLSEQ+KREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240
           VGVKMGGKDVI+MRQLRESN+EQ +VQK LK+LADD+FSEDAN  G FTS+  A KIKDE
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDAN--GGFTSNSVAAKIKDE 240

Query: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300
           YS+PWELRNAFAASIKPP+++HSK DEL  I++RRGGVD+GQSHNQWLSTISQSPNVISM
Sbjct: 241 YSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISM 300

Query: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360
           SFVPITSLMNG+RGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RH
Sbjct: 301 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRH 360

Query: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420
           R QASPSLKFTLMGP+LYVNTTKVD+ NRPVTG+RLFLEGKKSDHLA+HLQHLS+LPKTL
Sbjct: 361 RKQASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL 420

Query: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480
           QLSDDLSY+PIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLIDDDSASIVTKAWFEVKV
Sbjct: 421 QLSDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKV 480

Query: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540
           +GMRKVLFLRLGFSMVASA IRRS+WEGPSTTSRKSGLISTFISTRFS GLHQPE+QPKI
Sbjct: 481 IGMRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600
           ELNSAVYPDGPPLTSRAMKMLNVI+TKEMVRGPENSPGYWVVTGAKLCVEGGRIS+KVKY
Sbjct: 541 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 600

Query: 601 ALLTILSEDSMI 612
           +LLTILSEDSMI
Sbjct: 601 SLLTILSEDSMI 610

BLAST of MC03g_new0335 vs. NCBI nr
Match: XP_004146657.1 (MACPF domain-containing protein NSL1 [Cucumis sativus] >KGN64702.1 hypothetical protein Csa_013635 [Cucumis sativus])

HSP 1 Score: 1107 bits (2864), Expect = 0.0
Identity = 544/612 (88.89%), Postives = 582/612 (95.10%), Query Frame = 0

Query: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60
           MSFN++RF+PQSAAEKAVSV+GFGYDLCND+RLSSCKPGPSGSKLI +D TR++DLVLP 
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120
           G+VVPNV TSI CDKGERTRFRSDVISFNQMSE  NQQL LSGKIPSGFFN MF  KG W
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGST+SLAFDGCFITLYNIELE+SHITLSEQ+KREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240
           VGVKMGGKDVI+MRQLRESN+EQ +VQK LK+LADD+FSEDAN  G  TS+P A KIKDE
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDAN--GGLTSNPVAAKIKDE 240

Query: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300
           YS+PWELRNAFAASIKPP+++HSK DEL  I++RRGGVD+GQSHNQWLSTISQSPNVISM
Sbjct: 241 YSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISM 300

Query: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360
           SFVPITSLMNG+RGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RH
Sbjct: 301 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRH 360

Query: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420
           R QASPSLKFTLMGP+LYVNTTKVD+ N PVTG+RLFLEGKKSDHLA+HLQHLS+LPKTL
Sbjct: 361 RKQASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL 420

Query: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480
           QLSDDLSY+PIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVK 
Sbjct: 421 QLSDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKG 480

Query: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540
           +GMRKVLFLRLGFSMVASA IRRS+WEGPSTTSRKSGLISTFISTRFS GLHQPE+QPKI
Sbjct: 481 IGMRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600
           ELNSAVYPDGPPLTSRAMKMLNVI+TKEMVRGPENSPGYWVVTGAKLCVEGGRIS+KVKY
Sbjct: 541 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 600

Query: 601 ALLTILSEDSMI 612
           +LLTILSEDSMI
Sbjct: 601 SLLTILSEDSMI 610

BLAST of MC03g_new0335 vs. NCBI nr
Match: XP_022994356.1 (MACPF domain-containing protein NSL1 [Cucurbita maxima])

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 542/612 (88.56%), Postives = 581/612 (94.93%), Query Frame = 0

Query: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60
           MSFNV+RFDPQSAAEKAVSV+GFG+DLCND+RLSSCKPGPSGSKLI +D TRTKDLVLP 
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120
           G+VVPNVS SIKCDKGERTRFRSDVISFNQMSE FNQQL LSGKIPS FFN+MF  KG W
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS+KSL FDGCFITLYNIELERSHITLSEQ+KREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240
           VGVKMGGKDVI+MRQLRESN+EQ EVQK LKQLAD+RFSEDAN  G F S+ AA KIKDE
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQTEVQKKLKQLADERFSEDAN--GGFMSNSAAAKIKDE 240

Query: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300
           YS+PW+LR+AFAASIKPPI++HSK DELM I+VRRGGVD+GQSHNQWLST+SQSPNVI M
Sbjct: 241 YSMPWQLRHAFAASIKPPIITHSKGDELMTIYVRRGGVDVGQSHNQWLSTVSQSPNVILM 300

Query: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360
           SFVPITSLMNG+RGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH
Sbjct: 301 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360

Query: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420
           R QASPSLKFTLMGP+LYVNTTKVD+ +RPVTG+RLFLEGKKSDHLA+HLQHLS+LPKTL
Sbjct: 361 RKQASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL 420

Query: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480
           QLSDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLIDDD+ASIVTKAWFEVKV
Sbjct: 421 QLSDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKV 480

Query: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540
           +GMRKVLFLRLGFS VASA+IRRSEWEGPST+SRKSGLISTFISTRFSTGL QPE QPKI
Sbjct: 481 IGMRKVLFLRLGFSTVASAMIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600
           ELNSAVYPDGPPLT RAMKMLN+I+TKEMVRGPENSPGYWVVTGAKLCVEG RIS+KVKY
Sbjct: 541 ELNSAVYPDGPPLTVRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKY 600

Query: 601 ALLTILSEDSMI 612
           +LLTILSEDSMI
Sbjct: 601 SLLTILSEDSMI 610

BLAST of MC03g_new0335 vs. ExPASy TrEMBL
Match: A0A6J1CEM3 (MACPF domain-containing protein NSL1 OS=Momordica charantia OX=3673 GN=LOC111010810 PE=4 SV=1)

HSP 1 Score: 1226 bits (3172), Expect = 0.0
Identity = 612/612 (100.00%), Postives = 612/612 (100.00%), Query Frame = 0

Query: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60
           MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP
Sbjct: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60

Query: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120
           GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW
Sbjct: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120

Query: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240
           VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE
Sbjct: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240

Query: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300
           YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM
Sbjct: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300

Query: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360
           SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH
Sbjct: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360

Query: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420
           RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL
Sbjct: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420

Query: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480
           QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV
Sbjct: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480

Query: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540
           VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI
Sbjct: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600
           ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY
Sbjct: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600

Query: 601 ALLTILSEDSMI 612
           ALLTILSEDSMI
Sbjct: 601 ALLTILSEDSMI 612

BLAST of MC03g_new0335 vs. ExPASy TrEMBL
Match: A0A5D3DSF4 (MACPF domain-containing protein NSL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G001020 PE=4 SV=1)

HSP 1 Score: 1110 bits (2871), Expect = 0.0
Identity = 545/612 (89.05%), Postives = 584/612 (95.42%), Query Frame = 0

Query: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60
           MSFN++RF+PQSAAEKAVSV+GFGYDLCND+RLSSCKPGPSGSKLI +D TR++DLVLP 
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120
           G+VVPNV TSI CDKGERTRFRSDVISFNQMSE FNQQL LSGKIPSGFFN MF  KG W
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGST+SLAFDGCFITLYNIELE+SHITLSEQ+KREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240
           VGVKMGGKDVI+MRQLRESN+EQ +VQK LK+LADD+FSEDAN  G FTS+  A KIKDE
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDAN--GGFTSNSVAAKIKDE 240

Query: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300
           YS+PWELRNAFAASIKPP+++HSK DEL  I++RRGGVD+GQSHNQWLSTISQSPNVISM
Sbjct: 241 YSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISM 300

Query: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360
           SFVPITSLMNG+RGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RH
Sbjct: 301 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRH 360

Query: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420
           R QASPSLKFTLMGP+LYVNTTKVD+ NRPVTG+RLFLEGKKSDHLA+HLQHLS+LPKTL
Sbjct: 361 RKQASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL 420

Query: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480
           QLSDDLSY+PIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLIDDDSASIVTKAWFEVKV
Sbjct: 421 QLSDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKV 480

Query: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540
           +GMRKVLFLRLGFSMVASA IRRS+WEGPSTTSRKSGLISTFISTRFS GLHQPE+QPKI
Sbjct: 481 IGMRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600
           ELNSAVYPDGPPLTSRAMKMLNVI+TKEMVRGPENSPGYWVVTGAKLCVEGGRIS+KVKY
Sbjct: 541 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 600

Query: 601 ALLTILSEDSMI 612
           +LLTILSEDSMI
Sbjct: 601 SLLTILSEDSMI 610

BLAST of MC03g_new0335 vs. ExPASy TrEMBL
Match: A0A0A0LV66 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G075560 PE=4 SV=1)

HSP 1 Score: 1107 bits (2864), Expect = 0.0
Identity = 544/612 (88.89%), Postives = 582/612 (95.10%), Query Frame = 0

Query: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60
           MSFN++RF+PQSAAEKAVSV+GFGYDLCND+RLSSCKPGPSGSKLI +D TR++DLVLP 
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120
           G+VVPNV TSI CDKGERTRFRSDVISFNQMSE  NQQL LSGKIPSGFFN MF  KG W
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGST+SLAFDGCFITLYNIELE+SHITLSEQ+KREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240
           VGVKMGGKDVI+MRQLRESN+EQ +VQK LK+LADD+FSEDAN  G  TS+P A KIKDE
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDAN--GGLTSNPVAAKIKDE 240

Query: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300
           YS+PWELRNAFAASIKPP+++HSK DEL  I++RRGGVD+GQSHNQWLSTISQSPNVISM
Sbjct: 241 YSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISM 300

Query: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360
           SFVPITSLMNG+RGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RH
Sbjct: 301 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRH 360

Query: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420
           R QASPSLKFTLMGP+LYVNTTKVD+ N PVTG+RLFLEGKKSDHLA+HLQHLS+LPKTL
Sbjct: 361 RKQASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL 420

Query: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480
           QLSDDLSY+PIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVK 
Sbjct: 421 QLSDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKG 480

Query: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540
           +GMRKVLFLRLGFSMVASA IRRS+WEGPSTTSRKSGLISTFISTRFS GLHQPE+QPKI
Sbjct: 481 IGMRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600
           ELNSAVYPDGPPLTSRAMKMLNVI+TKEMVRGPENSPGYWVVTGAKLCVEGGRIS+KVKY
Sbjct: 541 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 600

Query: 601 ALLTILSEDSMI 612
           +LLTILSEDSMI
Sbjct: 601 SLLTILSEDSMI 610

BLAST of MC03g_new0335 vs. ExPASy TrEMBL
Match: A0A6J1JYX1 (MACPF domain-containing protein NSL1 OS=Cucurbita maxima OX=3661 GN=LOC111490102 PE=4 SV=1)

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 542/612 (88.56%), Postives = 581/612 (94.93%), Query Frame = 0

Query: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60
           MSFNV+RFDPQSAAEKAVSV+GFG+DLCND+RLSSCKPGPSGSKLI +D TRTKDLVLP 
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120
           G+VVPNVS SIKCDKGERTRFRSDVISFNQMSE FNQQL LSGKIPS FFN+MF  KG W
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS+KSL FDGCFITLYNIELERSHITLSEQ+KREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240
           VGVKMGGKDVI+MRQLRESN+EQ EVQK LKQLAD+RFSEDAN  G F S+ AA KIKDE
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQTEVQKKLKQLADERFSEDAN--GGFMSNSAAAKIKDE 240

Query: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300
           YS+PW+LR+AFAASIKPPI++HSK DELM I+VRRGGVD+GQSHNQWLST+SQSPNVI M
Sbjct: 241 YSMPWQLRHAFAASIKPPIITHSKGDELMTIYVRRGGVDVGQSHNQWLSTVSQSPNVILM 300

Query: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360
           SFVPITSLMNG+RGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH
Sbjct: 301 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360

Query: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420
           R QASPSLKFTLMGP+LYVNTTKVD+ +RPVTG+RLFLEGKKSDHLA+HLQHLS+LPKTL
Sbjct: 361 RKQASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL 420

Query: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480
           QLSDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLIDDD+ASIVTKAWFEVKV
Sbjct: 421 QLSDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKV 480

Query: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540
           +GMRKVLFLRLGFS VASA+IRRSEWEGPST+SRKSGLISTFISTRFSTGL QPE QPKI
Sbjct: 481 IGMRKVLFLRLGFSTVASAMIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600
           ELNSAVYPDGPPLT RAMKMLN+I+TKEMVRGPENSPGYWVVTGAKLCVEG RIS+KVKY
Sbjct: 541 ELNSAVYPDGPPLTVRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKY 600

Query: 601 ALLTILSEDSMI 612
           +LLTILSEDSMI
Sbjct: 601 SLLTILSEDSMI 610

BLAST of MC03g_new0335 vs. ExPASy TrEMBL
Match: A0A6J1GVB1 (MACPF domain-containing protein NSL1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457211 PE=4 SV=1)

HSP 1 Score: 1090 bits (2819), Expect = 0.0
Identity = 537/612 (87.75%), Postives = 577/612 (94.28%), Query Frame = 0

Query: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60
           MSFNV+RFDPQSAAEKAVSV+GFG+DLCND+RLSSCKPGPSGSKLI +D TRTKDLVLP 
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120
           G+VVPNVS SIKCDKGERTRFRSDV+SFNQMSE FNQQL LSGKIPS FFN+MF  KG W
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVVSFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS+KSL FDGCFITLYNIELERSHITLSEQ+KREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240
           VGVKMGGKDVI+MRQLRESN+EQ EVQK LKQLAD+RFSED N  G F S+  A KIKDE
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDVN--GGFMSNSTAAKIKDE 240

Query: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300
           YS+PW+LR+AFAASIKPPI++HSK DEL  I+VRRGGVD+GQSHNQWLST+SQSPNVISM
Sbjct: 241 YSMPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISM 300

Query: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360
           SFVPITSL+NG+RGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATR 
Sbjct: 301 SFVPITSLLNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRL 360

Query: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420
           R QASPSLKFTLMGP+LYVNTTKVD+ +RPVTG+RLFLEGKKSDHLA+HLQHLS+LPKT+
Sbjct: 361 RKQASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTI 420

Query: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480
           QLSDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLIDDD+ASIVTKAWFEVKV
Sbjct: 421 QLSDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKV 480

Query: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540
           +GMRKVLFLRLGFS VASA IRRSEWEGPST+SRKSGLISTFISTRFSTGL QPE QPKI
Sbjct: 481 IGMRKVLFLRLGFSTVASAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600
           ELNSAVYPDGPPLT RAMKMLNVI+TKEMVRGPENSPGYWVVTGAKLCVEG RIS+KVKY
Sbjct: 541 ELNSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKY 600

Query: 601 ALLTILSEDSMI 612
           +LLTILSEDSMI
Sbjct: 601 SLLTILSEDSMI 610

BLAST of MC03g_new0335 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 797.3 bits (2058), Expect = 8.3e-231
Identity = 393/613 (64.11%), Postives = 486/613 (79.28%), Query Frame = 0

Query: 4   NVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPPGIV 63
           N  R D  SAAEKAVSV+G GYDLC+D+R S+CK  P GS+L+ +DPTR +DL+ P GIV
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNWQND 123
           V NVS+SIKCDKGERTR RSD++SFNQMSE+FNQ +CLSGKIPSG FN MF F   W  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S K+LA+DG FI+LY++E+ R  +TL +++KREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDEYSV 243
            MGGKDVI+++Q+R+SN E  E+QK LK   D+RF  D   + S  S   + K K+E  +
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPAS-VYSGKPKEENLL 244

Query: 244 PWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISMSFV 303
            W L+  F  S+   +V H+K++E+M++ +RRGGVD+GQSH +WLST+SQ+PNVISM FV
Sbjct: 245 QWGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFV 304

Query: 304 PITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRNQ 363
           PITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R   Q
Sbjct: 305 PITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQ 364

Query: 364 ASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTLQLS 423
           +SPSL+F+LMGP+LYVNT+KVD+  RPVTG+R FLEGKK +HLA+HLQHLS+ P +L LS
Sbjct: 365 SSPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLS 424

Query: 424 DDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKVVGM 483
            D +Y+PI+EP EKGY+ PVKW IFSHVCT PV+YNG+   DD+ASIVTKAW EVK +GM
Sbjct: 425 HDDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGM 484

Query: 484 RKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLH----QPEKQPK 543
           RKVLFLRLGFS+ ASAV R+S W+  ST SRKSG+ S  ISTR STGL       + Q K
Sbjct: 485 RKVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSK 544

Query: 544 IELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVK 603
           I++NSAVYP GP    +  K+L++++TKE++RGPE  PGYWVVTGAKLCVE G+IS+K K
Sbjct: 545 IDINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAK 604

Query: 604 YALLTILSEDSMI 613
           Y+LLT++SEDS++
Sbjct: 605 YSLLTVISEDSLV 612

BLAST of MC03g_new0335 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 622.1 bits (1603), Expect = 4.8e-178
Identity = 319/611 (52.21%), Postives = 423/611 (69.23%), Query Frame = 0

Query: 7   RFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHL-DPTRTKDLVLPPGIVVP 66
           R     AAE A+  +G GYDL  D+RL  CK G   S+L+ + +     ++VLP GI +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNWQNDAG 126
           NVS SIKCDKGER RFRSD++ F QM+EQFNQ+L L+GKIPSG FNAMF F   WQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            TK+LAFDG FI+LY++ L++S + L E +K+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDA--NATGSFTSHPAAPKIKDEYSV 246
           GGKDVIY +Q   S L+  ++QK LK++AD RF E +  + TGS     A+ K++ +   
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGS-ERVQASSKVETK--- 243

Query: 247 PWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVD-IGQSHNQWLSTISQSPNVISMSF 306
             E R  FA +    + S++  ++ + +  RRGG D     HN+WL T+   P+VISMSF
Sbjct: 244 --EQRLRFADT--SSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSF 303

Query: 307 VPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRN 366
           +PITSL+NG+ G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL  + + 
Sbjct: 304 IPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQ 363

Query: 367 QASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTLQL 426
           Q+  SL+F+  GP+LYVNTT VD   RP+TG+RL+LEG++S+ LA+HLQHLSSLPK  QL
Sbjct: 364 QSCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQL 423

Query: 427 SDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKVVG 486
            DDL+     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G
Sbjct: 424 EDDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHG 483

Query: 487 MRKVLFLRLGFSMVASA-VIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKIE 546
            + VLFLRL FS V  A +++ SEW+     + KSGLIST IS  F+     P +   + 
Sbjct: 484 FKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVN 543

Query: 547 LNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKYA 606
           +NSA+YP GPP+ ++A K+L  ++T EM RGP+ SPGYWVV+GA+L VE G+IS+KVKY+
Sbjct: 544 INSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYS 600

Query: 607 LLTILSEDSMI 613
           L T +  D +I
Sbjct: 604 LFTPILGDEVI 600

BLAST of MC03g_new0335 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 459.1 bits (1180), Expect = 5.4e-129
Identity = 251/621 (40.42%), Postives = 382/621 (61.51%), Query Frame = 0

Query: 9   DPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGS---KLIHLDPTRTKDLVLPPGIVVP 68
           D     E AV  LG G+DL  D RL  CK G   +   +L+ LD T+ ++L +P   V  
Sbjct: 4   DGGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQ 63

Query: 69  NVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFK-GNWQNDA 128
           NVS  I CDKGERTRFRSD++ FN+MSE FNQ+  ++GKIPSG FNA F F+ G+W  DA
Sbjct: 64  NVSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDA 123

Query: 129 GSTKSLAFDGCFITLYNIELER-SHITLSEQLKREVPSSWDPAALAEFIEKYGTHVVVGV 188
            + KSL  D   +TL+N+ +   + + L+++++  VPSSWDP  LA FIE+YGTHV+ GV
Sbjct: 124 ANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGV 183

Query: 189 KMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFS-----EDANATGSFTSHPAAPKIK 248
            +GG+DV+ +RQ + S+L+ + ++  L  L D  F+            ++    + PK  
Sbjct: 184 SVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFP 243

Query: 249 DEYSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVI 308
           + ++V ++ +   A +      S +  + +  I  +RGG    +SH++WL T+   P+ I
Sbjct: 244 EAFNV-FDDKQTVAFN----NFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAI 303

Query: 309 SMSFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-A 368
           + +F+PITSL+  + G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A
Sbjct: 304 NFNFIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGA 363

Query: 369 TRHRNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLP 428
             +   A P+L    MGP+LYVNTT V ++  PVTG+R FLEGKK + LA+HLQHL +  
Sbjct: 364 APNMASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTR 423

Query: 429 KTL--QLSDDLSYKPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYNGSLI--------DDD 488
            T+  +++D+  ++  D+  +   YFEP+    FSHVCT PV+Y+ + I         +D
Sbjct: 424 TTVGEKITDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQND 483

Query: 489 SASIVTKAWFEVKVVGMRKVLFLRLGFSMVASAVIRRSEW-EGPSTTSRKSGLISTFIST 548
            A IVT A  EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+    
Sbjct: 484 VAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMP 543

Query: 549 RFSTGLHQ---PEKQPKIELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVV 604
             S  +H     + + ++ L+S V+P GPP+ +   K++  ++  ++ RGP++SPG+W+V
Sbjct: 544 LTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLV 603

BLAST of MC03g_new0335 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 427.9 bits (1099), Expect = 1.3e-119
Identity = 239/602 (39.70%), Postives = 350/602 (58.14%), Query Frame = 0

Query: 17  AVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPPGIVVPNVSTSIKCDKG 76
           A+  LG G+D+ +D+RL  CK  P GS+L+ ++  + +DL L  G ++PNV   I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNWQNDAGSTKSLAFDGCF 136
                R  V SF++M+E+FN +  + G IP G FNAMFN+ G+WQ DA STKSLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIYMRQL 196
           I LY+++L +  + L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+Y+RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDEYSVPWELRNAFAASIK 256
           + S L  +E++  +  +   RF E                             A + SI 
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHE-----------------------------AESQSIT 263

Query: 257 PPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISMSFVPITSLMNGIRGNG 316
            P+    K  ++  IF RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ G+ G  
Sbjct: 264 GPL--KYKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLR 323

Query: 317 FLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRNQASPSLKFTLMGPR 376
            L+ A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + +     SL+F+LMGP+
Sbjct: 324 HLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPK 383

Query: 377 LYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTLQLSDDLSYKPIDEPEE 436
           L+++  +V    +PVTG+RL LEG K + L++HLQHL SLPK LQ   D S+ PI  P+ 
Sbjct: 384 LFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKW 443

Query: 437 KG-------YFEPVKWSIFSHVCTAPVEYNGSLIDD-DSASIVTKAWFEVKVVGMRKVLF 496
           +G       +FEP+KW  FSHV T+P+E+  + I D     IVT A   V   G + VL 
Sbjct: 444 QGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLH 503

Query: 497 LRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKIELNSAVYP 556
           L+L FS V    IRRS W+                        H P     +  +  + P
Sbjct: 504 LKLLFSKVPGCTIRRSVWD------------------------HTP-----VASSGRLEP 559

Query: 557 DGPPLTS-------RAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKYA 604
            GP  +S       ++ K+  ++++ EM++GP++ PG+W+VTGAKL VE G+I ++VKY+
Sbjct: 564 GGPSTSSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYS 559

BLAST of MC03g_new0335 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 323.9 bits (829), Expect = 2.7e-88
Identity = 170/328 (51.83%), Postives = 226/328 (68.90%), Query Frame = 0

Query: 7   RFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHL-DPTRTKDLVLPPGIVVP 66
           R     AAE A+  +G GYDL  D+RL  CK G   S+L+ + +     ++VLP GI +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNWQNDAG 126
           NVS SIKCDKGER RFRSD++ F QM+EQFNQ+L L+GKIPSG FNAMF F   WQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            TK+LAFDG FI+LY++ L++S + L E +K+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDA--NATGSFTSHPAAPKIKDEYSV 246
           GGKDVIY +Q   S L+  ++QK LK++AD RF E +  + TGS     A+ K++ +   
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGS-ERVQASSKVETK--- 243

Query: 247 PWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVD-IGQSHNQWLSTISQSPNVISMSF 306
             E R  FA +    + S++  ++ + +  RRGG D     HN+WL T+   P+VISMSF
Sbjct: 244 --EQRLRFADT--SSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSF 303

Query: 307 VPITSLMNGIRGNGFLSHAVNLYLRYKP 331
           +PITSL+NG+ G+GFLSHA+NLYLR KP
Sbjct: 304 IPITSLLNGVPGSGFLSHAINLYLRCKP 323